CN104921838A - Constant temperature and humidity perfusion device for observation of mesentery microcirculation and application of constant temperature and humidity perfusion device - Google Patents
Constant temperature and humidity perfusion device for observation of mesentery microcirculation and application of constant temperature and humidity perfusion device Download PDFInfo
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- CN104921838A CN104921838A CN201510436733.4A CN201510436733A CN104921838A CN 104921838 A CN104921838 A CN 104921838A CN 201510436733 A CN201510436733 A CN 201510436733A CN 104921838 A CN104921838 A CN 104921838A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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Abstract
The invention provides a constant temperature and humidity perfusion device for observation of mesentery microcirculation. The constant temperature and humidity perfusion device comprises a perfusion box, a fixing plate and a temperature control device. The invention provides the application of the constant temperature and humidity perfusion device for microscopic observation of animal mesentery microcirculation at the same time. The constant temperature and humidity perfusion device is small in size and constant in temperature and humidity, a mesentery sample can be conveniently maintained clean, an experiment animal can be firmly fixed, and the constant temperature and humidity perfusion device is in close cooperation with a microscope.
Description
Technical field
The invention belongs to medical microscopic viewing instrument field, be specifically related to a kind of Mesentery microcirculation observation constant temperature and humidity perfusion device and application thereof.
Background technology
Microcirculation district is the place that mass exchange is directly carried out in blood and tissue fluid, and its blood circumstance can be observed by means of microscope.Organizing of mesentery position is thin, is suitable for easypro contracting and the blood circumstance of directly observing microcirculation district blood vessel.
The constant temperature perfusion device volume that existing Mesentery microcirculation is observed is larger, match with the microcirculation microscope of selling in the market, because volume is greatly not easily laid, can not at common biological microscope, especially the high-end microscope such as Bu Laide multinomial projection microscope system uses, have impact on the fineness of Microcirculatory Observations.The microcirculation microscope resolution of selling in the market is less, function singleness, is mainly used in the gross examination of microcirculation form, fluidised form and surrounding shape body, can not carries out more deep, careful observational study to microcirculation cytorheology.Cloth Rider multinomial projection microscope system and part special biological microscope resolution high, the former has the multiple optical function such as difference, dark ground, bright field, polarisation, Midst density bright field, continuous videos amplification, except to except the conventional microcirculation inspections such as microcirculation form, fluidised form, ambient state, also the rheological behaviour of the erythrocyte in microcirculation cytorheology, platelet, leukocyte, blood plasma is observed and quantitative analysis, but in prior art, also do not occur the device of the animal intestine mesentery Microcirculatory Observations supporting with it.
Chinese patent " rat mesenteria microcirculation observation constant-temperature perfusion device " (patent No. ZL2005201441671) provides a kind of constant temperature perfusion device for observation experiment, but this device observes site can not realize constant temperature and humidity, and this device is provided with the structures such as sliding door, device overall volume is excessive, not easily be placed on microscopic examination platform, connection that can not be closely firm with microscope.
Summary of the invention
The invention provides that a kind of volume is little, constant temperature and humidity, be convenient to keep mesentery sample clean, can laboratory animal be fixedly secured and perfusion device close-fitting with microscope, the present invention provides the application of this device simultaneously.
The technical scheme that the present invention solves the employing of its technical problem is:
A kind of Rat Mesenteric Microcirculation is observed and is used constant temperature and humidity perfusion device, comprises perfusion box, fixing head and temperature control equipment;
Described perfusion box comprises observation area, the thermal treatment zone and clear area, arranges Microcirculatory Observations window in described observation area, and described Microcirculatory Observations window higher than place, observation area plane and printing opacity, arranges temperature-sensing probe in described observation area; Arrange electrothermal tube in the described thermal treatment zone, the described thermal treatment zone is communicated with observation area, arranges connectivity slot between the thermal treatment zone and observation area;
The bolt described fixing head being arranged more than one screw and coordinate with described screw, preferably arrange more than four and have at least four to be evenly arranged on fixing head corner, also arrange holding the observation draw-in groove of observation window outer and for block connectivity slot edge and be positioned at the fixture block of fixing head bottom surface;
Described temperature control equipment is connected by wire with electrothermal tube and temperature-sensing probe.
Preferably, described perfusion box also comprises the fixed area being arranged on outside, and the outer of described fixed area arranges the holddown groove mated with microscope carrier sample slice clamper fixing threaded hole.
Preferably, the opposite edge that draw-in groove observed by described fixing head arranges the fixed card slot mated with microscope handel.
Preferably, described Microcirculatory Observations window is fixedly connected with the base plate of observation area by joint pin, and described joint pin is ground glass material.
Preferably, described perfusion box arranges temperature controlled region, and electrothermal tube is connected with temperature controller through temperature controlled region with the wire of temperature-sensing probe, and avoiding the junction point of wire and electrothermal tube and temperature probe to expose affects service life in atmosphere.
The present invention provides the application of described perfusion device simultaneously, and it is for microexamination animal Mesentery microcirculation.
Further, for Microcirculatory Observations that the multinomial microscopic system of Bu Laide carries out.
The model that temperature control equipment of the present invention selects Kai Hua Intelligent Instrument Watch Factory to produce is TDA8002 type temperature controller.
Described observation window is the good glass of light transmission or resin, for the light source through microscopic examination hole.
Beneficial effect of the present invention is:
(1) electrothermal tube, temperature-sensing probe and temperature control equipment is utilized to keep the temperature and humidity in perfusion device constant;
(2) volume is little, is conveniently placed on microscopical observation platform, is easy to mobile, flexible operation;
(3) the observation draw-in groove on fixing head is realized and being connected of perfusion box in the outer of joint pin by holding, and connect and closely stablize, fixing head covers the evaporation that can reduce solution in perfusion box above perfusion box;
(4) fixture block of fixing head coordinates with connectivity slot, and fixture block is stuck in two sides of connectivity slot, prevents fixing head and perfusion box from horizontally slipping;
(5) Microcirculatory Observations window is transparent light-transmitting materials, and joint pin is frosted material, and the light in microscopic examination hole effectively can pass through Microcirculatory Observations window, and joint pin reduces loss of light source, improves the definition observed;
(6) perfusion box and fixing head all arrange the groove with microscopes, with microscope stable connection;
(7) clear area provides the storage space of cleanout fluid, conveniently in experimentation, draws clean solution from clear area, and cleaning mesentery tissue, improves the definition of tissue.
Rat Mesenteric Microcirculation observation constant temperature and humidity perfusion device of the present invention, volume is little, and space layout utilizes rationally, can effectively reach constant temperature and humidity effect, be specially adapted to microscopic examination animal Mesentery microcirculation, have a good application prospect.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment 1;
Fig. 2 is the structural representation of embodiment 1 perfusion box;
Fig. 3 is the structural representation of embodiment 1;
Fig. 4 is the structural representation of embodiment 2 perfusion box.
In accompanying drawing, 1 perfusion box, 2 fixing heads, 3 temperature control equipments, 4 observation areas, 5 thermals treatment zone, 6 clear areas, 7 Microcirculatory Observations windows, 8 temperature-sensing probes, 9 electrothermal tubes, 10 screws, 11 observe draw-in groove, 12 fixed areas, 13 holddown grooves, 14 fixed card slots, 15 joint pins, 16 bolts, 17 connectivity slots, 18 temperature controlled region, 19 fixture blocks.
Detailed description of the invention
For enabling above-mentioned purpose of the present invention, feature and advantage become apparent more, below in conjunction with the drawings and specific embodiments in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
embodiment 1
As Figure 1-3, embodiment 1 comprises perfusion box 1, fixing head 2 and temperature control equipment 3;
Described perfusion box 1 comprises observation area 4, the thermal treatment zone 5, clear area 6, fixed area 12 and temperature controlled region 18, and described fixed area 12 is arranged on the side of the thermal treatment zone 5, and clear area 6 and fixed area 12 are arranged on the opposite side of the thermal treatment zone 5;
Temperature-sensing probe 8 and Microcirculatory Observations window 7 are set in described observation area 4, described Microcirculatory Observations window 7 is higher than observation area 4 place plane and printing opacity, described Microcirculatory Observations window 7 is fixedly connected with by the base plate of joint pin 15 with observation area 4, and described joint pin 15 is ground glass material; Arrange electrothermal tube 9 in the described thermal treatment zone 5, the described thermal treatment zone 5 is communicated with observation area 4, arranges connectivity slot 17 between the thermal treatment zone 5 and observation area 4; The outer of described fixed area 12 arranges the holddown groove 13 mated with microscope carrier sample slice clamper fixing threaded hole.
The bolt 16 described fixing head 2 being arranged more than one screw 10 and coordinate with described screw 10, described fixing head 2 side is provided with the observation draw-in groove 11 of holding in Microcirculatory Observations window 7 outer, opposite side arranges the fixed card slot 14 mated with microscope handel, the shape of described observation draw-in groove 11 and size and joint pin 15 outer shape, consistent size, the one side that described fixing head 2 contacts with perfusion box 1 is provided with fixture block 19, described fixture block 19 arranges two, when fixing head 2 covers on perfusion box 1, fixture block 19 is positioned at inside the both sides of connectivity slot 17, prevent the relative perfusion box 1 of fixing head 2 from sliding,
Described temperature control equipment 3 is connected with electrothermal tube 9 and temperature-sensing probe 8 wire, and the model that described temperature control equipment 3 selects Kai Hua Intelligent Instrument Watch Factory to produce is TDA8002 type temperature controller.
Described Microcirculatory Observations window 7 is the good glass of light transmission, for the light source through microscopic examination hole.
Electrothermal tube 9 is connected with temperature controller through temperature controlled region 18 with the wire of temperature-sensing probe 8, and avoiding the junction point of wire and electrothermal tube 9 and temperature probe 8 to expose affects service life in atmosphere.
Embodiment 1, for microexamination animal Mesentery microcirculation, is specially adapted to the Microcirculatory Observations that the multinomial microscopic system of Bu Laide carries out.During use, be placed on by perfusion box 1 on microscopical object stage, the holddown groove 13 of fixed area 12 is fastened in outside microscope carrier sample slice clamper fixing threaded hole, Microcirculatory Observations window 7 aligming microscope objective lens.In the thermal treatment zone 5, observation area 4 and clear area 6, add normal saline, open temperature control equipment 3, electrothermal tube 9 pairs of normal saline heat.
Laboratory animal is fixed on fixing head 2, with fixing band, animal is bound, and utilized by fixing band the screw 10 on fixing head 2 and bolt 16 to fix.Be placed on by fixing head 2 above perfusion box 1, fixed card slot 14 is stuck on microscope handel, observes draw-in groove 11 holding in joint pin 15 outer.
Be placed on by the mesentery of laboratory animal on Microcirculatory Observations window 7, clean with the normal saline flushing of clear area 6, the normal saline of the thermal treatment zone 5 and observation area 4 provides the environment of constant temperature and humidity for mesentery tissue, keeps the concordance inside and outside organizer and stability.Microcirculatory Observations window 7 is the good light-transmitting materials of light transmission, is convenient to microscope light source and passes, and improves the definition observed.
embodiment 2
As shown in Figure 4, composition graphs 1-3, embodiment 2 as different from Example 1, the fixed area 12 of embodiment 1 perfusion box 1 and the thermal treatment zone 5 in the same side of observation area 4, the fixed area 12 of embodiment 2 with the thermal treatment zone 5 respectively in the relative both sides of observation area 4; The fixing head 2 of embodiment 1 is provided with fixed card slot 14, and embodiment 2 does not arrange fixed card slot 14.
Embodiment 2 be applicable to laboratory animal volume larger time, fixing head 2 is not easily placed between microscope objective and the object stage of handel.During use, be placed on by perfusion box 1 on microscopical object stage, the holddown groove 13 of fixed area 12 is fastened in microscope carrier sample slice clamper fixing threaded hole coupling outer, Microcirculatory Observations window 7 aligming microscope objective lens; Fixing head 2 is placed on above the thermal treatment zone 5, observes draw-in groove 11 holding in joint pin 15 outer.
Other structures are identical with embodiment 1 with operational approach.
Embodiment 2 fixing, 2 to be arranged on perfusion box 1 and outside microscope, for large-area fixing head 2 provides sufficient space, can to place volume comparatively big experiment animal.
Claims (6)
1. Mesentery microcirculation is observed and is used a constant temperature and humidity perfusion device, it is characterized in that, comprises perfusion box (1), fixing head (2) and temperature control equipment (3);
Described perfusion box (1) comprises observation area (4), the thermal treatment zone (5) and clear area (6), Microcirculatory Observations window (7) is set in described observation area (4), described Microcirculatory Observations window (7) higher than observation area (4) place plane and printing opacity, arranges temperature-sensing probe (8) in described observation area (4); Arrange electrothermal tube (9) in the described thermal treatment zone (5), the described thermal treatment zone (5) are communicated with observation area (4), arrange connectivity slot (17) between the thermal treatment zone (5) and observation area (4);
The bolt (16) described fixing head (2) being arranged more than one screw (10) and coordinate with described screw, also arrange holding the observation draw-in groove (11) of observation window (4) outer and for block connectivity slot (17) edge and be positioned at the fixture block (19) of fixing head (2) bottom surface;
Described temperature control equipment (3) is connected by wire with electrothermal tube (9) and temperature-sensing probe (8).
2. perfusion device according to claim 1, is characterized in that, described perfusion box (1) also comprises the fixed area (12) be arranged on outside it, and the outer of described fixed area (12) arranges the holddown groove (13) be connected with microscope carrier.
3. perfusion device according to claim 1, is characterized in that, the opposite edge that draw-in groove (11) observed by described fixing head (2) arranges the fixed card slot (14) mated with microscope handel.
4. perfusion device according to claim 1, is characterized in that, described Microcirculatory Observations window (7) is fixedly connected with by the base plate of joint pin (15) with observation area (4), and described joint pin (15) is ground glass material.
5. the application of the perfusion device described in any one of claim 1-4, is characterized in that, for microexamination animal Mesentery microcirculation.
6. application according to claim 5, is characterized in that, for the Microcirculatory Observations that the multinomial microscopic system of Bu Laide carries out.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510436733.4A CN104921838B (en) | 2015-07-23 | 2015-07-23 | A kind of Mesentery microcirculation observation constant temperature and humidity perfusion device and its application |
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| CN201510436733.4A CN104921838B (en) | 2015-07-23 | 2015-07-23 | A kind of Mesentery microcirculation observation constant temperature and humidity perfusion device and its application |
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| CN104921838B CN104921838B (en) | 2017-06-06 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105676461A (en) * | 2016-04-05 | 2016-06-15 | 皖南医学院 | Animal mesentery microcirculation observation system |
| CN110037881A (en) * | 2019-05-16 | 2019-07-23 | 上海长征医院 | A kind of stage apparatus for the observation of mouse cremaster |
| CN113907907A (en) * | 2021-09-30 | 2022-01-11 | 广东省科学院健康医学研究所 | Mouse dorsal spine skin pleat window model system and microcirculation constant temperature control method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2922834Y (en) * | 2005-12-22 | 2007-07-18 | 覃建民 | Rat mesenteria microcirculation observation constant-temperature perfusion device |
| CN203841834U (en) * | 2014-04-15 | 2014-09-24 | 上海市同济医院 | Multifunctional constant-temperature operation perfusion device |
| CN104146789A (en) * | 2014-07-17 | 2014-11-19 | 梁建民 | Constant-temperature heating plate and experiment table |
| CN203953871U (en) * | 2014-07-31 | 2014-11-26 | 王晓萃 | Experiment is poured into fixture with rat |
| CN204839817U (en) * | 2015-07-23 | 2015-12-09 | 河北医科大学第一医院 | Mesenterium microcirculation is observed with constant temperature and humidity perfusion device |
-
2015
- 2015-07-23 CN CN201510436733.4A patent/CN104921838B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2922834Y (en) * | 2005-12-22 | 2007-07-18 | 覃建民 | Rat mesenteria microcirculation observation constant-temperature perfusion device |
| CN203841834U (en) * | 2014-04-15 | 2014-09-24 | 上海市同济医院 | Multifunctional constant-temperature operation perfusion device |
| CN104146789A (en) * | 2014-07-17 | 2014-11-19 | 梁建民 | Constant-temperature heating plate and experiment table |
| CN203953871U (en) * | 2014-07-31 | 2014-11-26 | 王晓萃 | Experiment is poured into fixture with rat |
| CN204839817U (en) * | 2015-07-23 | 2015-12-09 | 河北医科大学第一医院 | Mesenterium microcirculation is observed with constant temperature and humidity perfusion device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105676461A (en) * | 2016-04-05 | 2016-06-15 | 皖南医学院 | Animal mesentery microcirculation observation system |
| CN110037881A (en) * | 2019-05-16 | 2019-07-23 | 上海长征医院 | A kind of stage apparatus for the observation of mouse cremaster |
| CN113907907A (en) * | 2021-09-30 | 2022-01-11 | 广东省科学院健康医学研究所 | Mouse dorsal spine skin pleat window model system and microcirculation constant temperature control method |
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| CN104921838B (en) | 2017-06-06 |
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