CN2922834Y

CN2922834Y - Rat mesenteria microcirculation observation constant-temperature perfusion device

Info

Publication number: CN2922834Y
Application number: CN 200520144167
Authority: CN
Inventors: 覃建民; 杨玉梅; 陈彩; 徐庆军
Original assignee: Individual
Current assignee: Individual
Priority date: 2005-12-22
Filing date: 2005-12-22
Publication date: 2007-07-18
Anticipated expiration: 2015-12-22

Abstract

This model utility relates to a constant temperature perfusion device for observing mesenteric microcirculation in rats and includes a constant temperature box, the two sides of the constant temperature box body are provided with a sliding door, the top end of the constant temperature box is provided with a microscope hole, the constant temperature box is provided with a temperature sensor and a blast pipe, the blast pipe is provided with a radiator, the radiator is provided with a spiral glass pipe, the bottom of the constant temperature box is provided with animals fixation frame and intestine perfusion fixation box, the intestine perfusion fixation box comprises an outer groove box and an inner groove box, the wall of the inner groove box is provided a thermometer, a liquid outlet interlinked with the outer groove box and liquid inlet pipe connected with outer groove box, the liquid inlet pipe is connected with the spiral glass pipe through a rubber pipe, the inner groove box is provided with a translucidus pole, the wall of the outer groove box is provided with liquid outlet pipe and a luminescence source is provided within the outer groove box and in the intestine perfusion fixation box. This model utility is characterized as follows: simple operation, lower prices, less stimulation to the mesenteric blood vessels and provides a constant temperature, humidity and pressure condition for observing the mesenteric microcirculation.

Description

The rat mesentery microcirculation is observed and is used the constant temperature perfusion device

Technical field

This utility model relates to a kind of rat mesentery microcirculation observation constant temperature perfusion device.

Background technology

Mesentery is as thin as a wafer a kind of and transparent film sample tissue, simple and complete capillary network is arranged, can utilize the mesentery microcirculation to carry out many-sided research, need the intestinal tube perfusion device of a constant temperature similar, constant humidity, constant voltage in the observation, but do not have the constant temperature perfusion device that can use for observation experiment a few days ago on the market to interior environment.

Summary of the invention

The purpose of this utility model provides a kind of rat mesentery microcirculation observation constant temperature perfusion device easy and simple to handle, cheap, little to the mesentery vascular stimulation.

The purpose of this utility model is realized by following mode:

This utility model comprises a calorstat of making of lucite, respectively be provided with a sliding door in the both sides of calorstat casing, be provided with the microscope hole at the top of calorstat, on calorstat, be provided with temperature detector and blast pipe, on blast pipe, be provided with radiator, on radiator, be provided with spiral glass pipe, be provided with animal fixed mount and intestinal tube perfusion fixation box at the calorstat inner bottom part, intestinal tube perfusion fixation box is made of water jacket box and inside groove box, water jacket box and inside groove box all have lid, on the box wall of inside groove box, be provided with thermometer, the liquid outlet that communicates with the water jacket box and lead to the outer feed tube of water jacket box, feed tube is connected with spiral glass pipe with sebific duct, in the inside groove box, be provided with and the vertical printing opacity post in microscope hole, on the box wall of water jacket box, be provided with drain pipe, in the water jacket box, the top of intestinal tube perfusion fixation box is provided with cold light source.

The utility model has the advantages that: environment easy and simple to handle, cheap, little, that constant temperature, constant humidity, constant voltage can be provided for the observation of mesentery microcirculation the mesentery vascular stimulation.

Description of drawings

Fig. 1 is a structural representation of the present utility model; Fig. 2 is the structural representation of intestinal tube perfusion fixation box.

The specific embodiment

With reference to accompanying drawing, this utility model comprises a calorstat 1 of making of lucite, respectively be provided with a sliding door 8 in the both sides of calorstat 1 casing, 12, be provided with microscope hole 6 at the top of calorstat 1, on calorstat 1, be provided with temperature detector 5 and blast pipe 2, on blast pipe 2, be provided with radiator 3, on radiator 3, be provided with spiral glass pipe 13, be provided with animal fixed mount 7 and intestinal tube perfusion fixation box 11 at the calorstat inner bottom part, intestinal tube perfusion fixation box 11 is made of water jacket box 14 and inside groove box 19, water jacket box 14 and inside groove box 19 all have lid, on the box wall of inside groove box, be provided with thermometer 18, the liquid outlet 17 that communicates with the water jacket box and lead to the outer feed tube 20 of water jacket box, feed tube 20 usefulness sebific ducts 4 are connected with spiral glass pipe 13, in the inside groove box, be provided with and the vertical printing opacity post 15 in microscope hole, on the box wall of water jacket box, be provided with drain pipe 9, in the water jacket box, the top of intestinal tube perfusion fixation box is provided with cold light source 10.

In use, the calorstat overall fixed is on microscope carrier, its volume is decided according to microscope, the microscope shank inserts in the calorstat, one end of blast pipe docks with a hair-dryer, hair-dryer is connected with temperature controller, spiral glass pipe is connected with liquid containing bottle with sebific duct, open hair-dryer and regulate calorstat temperature to 38 ± 0.5 ℃, regulate the position of perfusate flow velocity and spiral glass pipe and radiator then, make the temperature of perfusate reach 38 ± 0.5 ℃, postanesthetic rat is put into propose intestinal tube gently after calorstat is performed the operation, mesentery vascularity situation detects by an unaided eye, selection has little blood vessel traveling, and under-finished position is put into intestinal tube perfusion fixation box and built lid as viewing area, open microscope transmitted light source and cold light source, regulate the microscope focal length and observe.

Claims

1, a kind of rat mesentery microcirculation is observed and is used the constant temperature perfusion device, comprise a calorstat of making of lucite, it is characterized in that: respectively be provided with a sliding door in the both sides of calorstat casing, be provided with the microscope hole at the top of calorstat, on calorstat, be provided with temperature detector and blast pipe, on blast pipe, be provided with radiator, on radiator, be provided with spiral glass pipe, be provided with animal fixed mount and intestinal tube perfusion fixation box at the calorstat inner bottom part, intestinal tube perfusion fixation box is made of water jacket box and inside groove box, water jacket box and inside groove box all have lid, on the box wall of inside groove box, be provided with thermometer, the liquid outlet that communicates with the water jacket box and lead to the outer feed tube of water jacket box, feed tube is connected with spiral glass pipe with sebific duct, in the inside groove box, be provided with and the vertical printing opacity post in microscope hole, on the box wall of water jacket box, be provided with drain pipe, in the water jacket box, the top of intestinal tube perfusion fixation box is provided with cold light source.