CN107418891A - A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system - Google Patents
A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system Download PDFInfo
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- CN107418891A CN107418891A CN201710858891.8A CN201710858891A CN107418891A CN 107418891 A CN107418891 A CN 107418891A CN 201710858891 A CN201710858891 A CN 201710858891A CN 107418891 A CN107418891 A CN 107418891A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Abstract
The invention discloses a kind of perfusion type bladder bacterial biof iotalm dynamic to be incubated simulation system, belongs to bacterial biof iotalm incubation system technical field.Perfusion type bladder bacterial biof iotalm dynamic, which is incubated simulation system, to be included：Storage of urine, peristaltic pump, box, urine collecting device and connecting tube are incubated, the storage of urine, the peristaltic pump, the incubation box and the urine collecting device are sequentially connected by the connecting tube；Wherein, the lid for being incubated box and including box body and being connected with the box body, the both sides of the box body offer inlet opening and fluid hole respectively, and the inlet opening is connected by the connecting tube with the outlet of the peristaltic pump, and the fluid hole is connected by the connecting tube with the urine collecting device.The present invention realizes the incubation conditions ensured sustained development and alterable of environment in simulation bladder so that Experiment intervention measure mobilism and variation are possibly realized.
Description
Technical field
The present invention relates to bacterial biof iotalm incubation system technical field, and in particular to a kind of perfusion type bladder bacterial biof iotalm
Dynamic is incubated simulation system.
Background technology
Bacterial biof iotalm (Bacteria lbiofilm, BF) be bacterium in order to survive, in dielectric surface in a manner of colony
It is embedded in the matrix net of Extracellular polymers (extraceUular polymeric substances, EPS) composition, is oneself
Construct " the umbrella system " of One function.BF is by Extracellular polymers, bacterium (bacterium or biofilm bacteria in film) and water
Divide three parts composition.Extracellular polymers are the bases for maintaining its 26S Proteasome Structure and Function, by the polysaccharide protein complex of bacterial secretory, carefully
The composition such as bacterium metabolite and absorption surrounding environment material.
Catheter correlation urinary tract infections (CAUTIs) is one of most important reason of nosocomial infection.Catheter surface biology
It is the reason for causing CAUTIs to cause a disease and cause resistance mostly important that film, which is formed,.
On the one hand catheter breaks through the physiological defense mechanism of human normal, adds external source as iatrogenic implant
Property pathogenic bacteria or conditioned pathogen infection chance, on the other hand turn into bacterial adhesion and field planting as foreign matter, and further
Form BF suitable carrier.Research shows, cause of disease in the protectiveness microenvironment and 60%-85% implanted object that BF is provided
Bacterium infected with interwoveness relation, be both bacterium infection acute attack or it is chronic repeatedly the reason for, and can induce immune response,
Form excessively rush inflammatory effector and endanger host.
In recent years, serious CAUTIs adverse events or case fatality rate increase year by year caused by bacterium infection, have had resulted in serious
Heath economics burden, its preventing and controlling shoulders heavy responsibilities.
Study BF incubation systems of the urine source property BF firstly the need of environment in structure simulation bladder.Previously the BF of research forms reality
Test and use quiescent culture method (such as test tube, porous culture plate) mostly, bacterium is under the conditions of static gas wave refrigerator in dielectric surface shape
It is also relatively easy into BF, intervention factor.The continuous growth and breeding of bacterium, BF constantly formed can consumed energy, the battalion in culture medium
Support material and be distributed uneven or gradual scarcity, opsonigenous substance constantly accumulation etc. can have a strong impact on bacterium and its biomembrane
Normal growth.Thus, different condition of culture (such as temperature, time, nutritional condition, hydrodynamics, media table noodles), culture
Base feed postition (add or flow and add), update cycle etc. may influence BF form and function.
Current existing incubation system is mostly static incubation, because uroflow environment is in dynamic all the time in bladder, therefore it is right
For bladder bacterium, the research method of static incubation can not really react its growing environment, sentence so as to influence final analysis
It is disconnected.
In view of this, it is special to propose the present invention.
The content of the invention
In order to solve the problems, such as that prior art is present, it is an object of the present invention to provide a kind of life of perfusion type bladder bacterium
Thing film dynamic is incubated simulation system, to solve the problems, such as that prior art can not actual response bladder environment of bacteria growth.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system, including：Storage of urine, peristaltic pump, it is incubated box, collection
Device and connecting tube are urinated, the connecting tube connects the storage of urine, the peristaltic pump, the incubation box and the urine collecting device successively
Connect；Wherein, the box that is incubated includes box body and the lid that is connected with the box body, the both sides of the box body offer respectively into
Fluid apertures and fluid hole, the inlet opening are connected by the connecting tube with the outlet of the peristaltic pump, and the fluid hole passes through institute
Connecting tube is stated to be connected with the urine collecting device.
The urine being stored in storage of urine is constantly pumped to and is incubated in box by the present invention by peristaltic pump, while by opening up
In the inlet opening and fluid hole that are incubated box box body both sides, urine in future self storage device forms continuous dynamic being incubated in box
Fluid environment, realizes the mobilism of bladder bacterial biof iotalm incubation conditions, more truly simulates the growth ring of bladder bacterium
Border.The present invention realizes the incubation conditions ensured sustained development and alterable of environment in simulation bladder so that Experiment intervention measure mobilism
It is possibly realized with variation.
Further, in preferred embodiments of the present invention, height of the above-mentioned inlet opening apart from the box body bottom is more than
Height of the fluid hole apart from the box body bottom.
The present invention in the both sides for being incubated box box body by setting inlet opening and the height of fluid hole and inlet opening to be higher than
Fluid apertures, form flow channel and flow pass with height fall so that being incubated has the artificial urine of stable volume in box,
And it can effectively prevent reflux.
Further, in preferred embodiments of the present invention, above-mentioned incubation box includes two horizontally spaced settings
The inlet opening and two horizontally spaced settings the fluid hole.
Further, in preferred embodiments of the present invention, above-mentioned incubation box also include respectively with the inlet opening and institute
The threading straight coupling of fluid hole cooperation is stated, the threading straight coupling is connected with the connecting tube.
Further, in preferred embodiments of the present invention, above-mentioned incubation box also includes multiple demarcation strips, multiple described points
Direction of the dividing plate along the inlet opening and the fluid hole line is arranged in the box body.
The present invention sets demarcation strip in incubation box, and tray interior is divided into multiple regions, ensures that artificial urine is uniform
Distribution, makes to be more nearly the true environment inside bladder in incubation box.
Further, in preferred embodiments of the present invention, above-mentioned incubation box is immunohistochemical wet box.
It is highly preferred that immunohistochemical wet box is the light tight box of black, to simulate the dark surrounds in human bladder, make
Whole simulation system is obtained closer to environment in real human bladder.In experiment, a whole set of simulation system is placed in 37 DEG C of perseverance
In warm incubator, formed and human body identical temperature environment.
Further, in preferred embodiments of the present invention, the outlet of above-mentioned storage of urine by the connecting tube with it is described
The entrance connection of peristaltic pump, the outlet of the peristaltic pump is connected by the connecting tube with the entrance of the incubation box, described to incubate
The outlet for educating box is connected by the connecting tube with the entrance of the urine collecting device.
Further, in preferred embodiments of the present invention, above-mentioned storage of urine and the urine collecting device are with sealing-plug
Glass triangle flask, the sealing-plug are connected with the connecting tube.
Further, in preferred embodiments of the present invention, above-mentioned connecting tube is silicone tube.
The invention has the advantages that：
(1) present invention establishes the stable fluid environment of dynamic, can state that more preferably simulation urine flows, more conform to
Uroflow environment in bladder, more real growing environment can be provided for bladder bacterium, be provided for medical research relatively reliable
Information.
(2) present invention can not only ensure that artificial urine produces continuous flow regime as continuous media, but also can
To simulate low hydrodynamic shear condition, to be advantageous to flcating germ and act on dielectric surface.
(3) flow of the invention, flow velocity are adjustable, can control the artificial total volume of urine and speed into system, so as to
The hydrouria of physiological status or the state (supplement or lack moisture) of reduction can be simulated, the few different rhythm and pace of moving things of more nights in daytime can
To simulate the pathological state of diuresis or oliguresis, and simulation persistent infection artificial urine environment.
(4) present invention can realize the Experiment intervention condition such as mobilism such as urine glucose concentration, pH value, salt ionic concentration
Adjustment, it is practical.
(5) all components of the invention belong to low value consumptive material, and disassembling, assembling and replacing is very convenient, and can sterilize repeatedly, repeat
Use.
(6) all material that the present invention uses is safe and reliable, no cytotoxicity, extremely meets the outer Experiment on Microbiology of analogue body
Condition.
Brief description of the drawings
Fig. 1 is the structural representation of the embodiment of the present invention；
Fig. 2 is the structural representation of the incubation box of the embodiment of the present invention；
Fig. 3 is the structural representation of the incubation box of the embodiment of the present invention；
Fig. 4 is the structural representation of the threading straight coupling of the embodiment of the present invention.
Arrow represents the flow direction of urine in figure.
In figure：100- dynamics are incubated simulation system；110- storage of urine；120- peristaltic pumps；130- is incubated box；131-
Box body；132- lids；133- inlet openings；134- fluid holes；135- demarcation strips；140- urine collecting devices；150- connecting tubes；
160- threading straight couplings；161- Part I；162- Part II；163- Part III.
Embodiment
The principle and feature of the present invention are described below in conjunction with accompanying drawing, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.Unreceipted actual conditions person in embodiment, the bar suggested according to normal condition or manufacturer
Part is carried out.Agents useful for same or the unreceipted production firm person of instrument, it is the conventional products that can be obtained by commercially available purchase.
Embodiment
Fig. 1 is refer to, perfusion type bladder bacterial biof iotalm dynamic, which is incubated simulation system 100, to be included：Storage of urine 110, wriggle
Pump 120, it is incubated box 130, urine collecting device 140 and connecting tube 150.Connecting tube 150 by storage of urine 110, peristaltic pump 120, be incubated box 130
It is sequentially connected with urine collecting device 140.Specifically, the outlet of storage of urine 110 is connected by connecting tube 150 with the entrance of peristaltic pump 120,
The outlet of peristaltic pump 120 is connected by connecting tube 150 with being incubated the entrance of box 130, and the outlet for being incubated box 130 passes through connecting tube
150 are connected with the entrance of urine collecting device 140.In the present embodiment, connecting tube 150 is silicone tube.The number of the connecting tube 150 of the present invention
Amount is not specifically limited, as the case may be accommodation.
Fig. 1 is refer to, storage of urine 110 includes vessel and the sealing-plug being arranged at vessel port.Sealing-plug has
Storage of urine 110 is connected by the through hole being engaged with connecting tube 150, connecting tube 150 through sealing-plug with peristaltic pump 120.Storage of urine
110 are used to store artificial urine, and uroflow is provided to be incubated box 130.In the present embodiment, storage of urine 110 is the glass with sealing-plug
Glass conical flask, its volume are 500mL.In other embodiments of the invention, storage of urine 110 can also be other salable appearances
Device.Urine collecting device 140 is identical with the structure of storage of urine 110, is also the glass triangle flask with sealing-plug, sealing-plug and connecting tube 150
Connection.
Fig. 1 is refer to, peristaltic pump 120 is connected with storage of urine 110 and incubation box 130 respectively by connecting tube 150, and it is acted on
In the artificial urine in storage of urine 110 is pumped to be incubated box 130 in.In the present embodiment, the peristaltic pump 120 used is card
Gadamer brand, its pump housing is compact, and reduction uses space, by knob manual governing and metering, no gate valve and seal, and Inlet and outlet water
It is convenient that pipe is changed, and its pump head is click-on design, is easy to dismount, and cleaning is convenient, and repeatable accuracy is high, and three-wheel rolls, and pulsation is small,
Liquid is run in pump line, is contacted pump line inwall, is not contacted the pump housing, safety non-pollution.In other embodiments of the invention,
The peristaltic pump 120 of other brands can be used.The present invention is not improved to the structure of peristaltic pump 120, therefore its concrete structure
It is not described herein, the peristaltic pump 120 that the present invention uses is existing peristaltic pump.
Fig. 2 and Fig. 3 are refer to, being incubated box 130 includes box body 131, the lid 132 being connected with box body 131 and be arranged at
Multiple demarcation strips 135 of box body 131.The both sides of box body 131 offer inlet opening 133 and fluid hole 134 respectively.Inlet opening 133
It is connected by connecting tube 150 with the outlet of peristaltic pump 120.Fluid hole 134 is connected by connecting tube 150 with urine collecting device 140.Feed liquor
Height of the hole 133 apart from the bottom of box body 131 is more than height of the fluid hole 134 apart from the bottom of box body 131, it is possible thereby to which being formed has
The flow channel and flow pass of the discrepancy in elevation, avoid urine reflux.In the present embodiment, inlet opening 133 and fluid hole 134 are two
It is individual, and correspond to each other, and two inlet openings 133 and two fluid holes 134 are intervally installed in the horizontal direction.One
In individual preferred embodiment, a height of 2cm in hole (apart from the bottom of box body 131) of inlet opening 133, holes distance 9cm.Fluid hole
The 134 a height of 1cm in hole (apart from the bottom of box body 131), holes distance 9cm.With two inlet openings, 133 corresponding two connecting tubes
150 are connected by three-way connection with being connected the connecting tube 150 of peristaltic pump 120, and similarly, fluid hole 134 is also such.
As shown in Fig. 2 direction of multiple demarcation strips 135 along inlet opening 133 and the line of fluid hole 134 is arranged at box body 131
It is interior.The embodiment shown in Fig. 2 has 4 demarcation strips 135, and in other embodiments of the invention, can be 3 can also be
More, specific number can be adjusted according to the size of box 130 is incubated.
Fig. 4 is refer to, is incubated the threading straight coupling that box 130 also includes with inlet opening 133 and fluid hole 134 coordinating respectively
160, threading straight coupling 160 is connected with connecting tube 150.The pipe of 160 generally linear type of threading straight coupling, its one end with
Connecting tube 150 is connected, and the other end, which is maintained at, to be incubated in box 130, its effect for connecting connecting tube 150 and being incubated box 130.In this reality
Apply in example, the structure of threading straight coupling 160 is as shown in figure 4, it mainly includes three parts：Part I 161 is substantially in cylinder
Body structure, its surface are provided with screw thread, and the part is connected with connecting tube 150；Part II 162 is middle annular protrusion, is being pacified
The outside of box body 131 is maintained at during dress, its effect to fixation；Part III 163 is also substantially in cylindrical structure, its surface light
Sliding, the part coordinates with inlet opening 133 or fluid hole 134, through the side wall of box body 131.
In another embodiment of the present invention, immunohistochemical wet box can be used by being incubated box 130, immunohistochemical wet box
Box body both sides open up inlet opening 133 and fluid hole 134, and are connected by threading straight coupling 160 with miscellaneous part.
The operation principle and operating process of the present invention are illustrated below.
The present invention is using threading straight coupling 160, three-way connection, silicone tube, peristaltic pump 120, incubation box 130, storage of urine
110 and urine collecting device 140, simulate uroflow environment in bladder and continue uroflow state, construct In vitro perfusion formula perfusion type bladder bacterium
Biomembrane dynamic is incubated simulation system 100.In use, power-on, peristaltic pump 120 runs well, by artificial urine slowly pump
Enter to be incubated box 130, be incubated artificial urine accumulation in box 130 and normally, keep certain volume, e.g., from about 500ml, it is continuous to form dynamic
Flowing environment.After the completion of experiment, power supply is closed, system shuts down.The present invention is using peristaltic pump 120 by storage of urine 110
Artificial urine is actively pumped into through flow channel and is incubated box 130, is formed and is incubated microenvironment, and artificial urine slowly flows through flow pass
Go out, eventually arrive at urine collecting device 140.Continuous flowing environment is established, realizes the mobilism of experimental condition.
Concrete operations flow is as follows：
(1) it is standby (except the pump housing of peristaltic pump 120) after all components high-temperature sterilization.Start experiment when, wear sterile gloves by
According to each component that is linked in sequence, check errorless.
(2) fill artificial urine about 500ml in storage of urine 110, open the power supply of peristaltic pump 120, artificial urine is from inflow entrance
Slowly it is pumped into and is incubated box 130.
(3) inflow of incubation box 130, outlet height drop are 1cm, remain the people of about 500ml stable volumes in it
Work urine.
(4) speed oozed in the flow pass observation artificial urine for being incubated box 130, adjusts (the flow rates of peristaltic pump 120
4-65ml/min), the flow and flow rate that experiment needs is reached.
(5) storage of urine 110 is changed in timing and urine collecting device 140, guarantee system are generally run.
(6) stop experiment, close power supply, remove component, sterilization is standby.
In summary, perfusion type perfusion type bladder bacterial biof iotalm of the invention dynamic is incubated simulation system 100 by being easy to
The laboratory equipment of acquisition, by designed, designed and assemble, prepared and simulated uroflow environment in human bladder as far as possible
Bacterial biof iotalm dynamic model, and continuous or change intervention factor, such as persistently artificial urine environment observation can be realized
The growth and development characteristicses that bacterial biof iotalm is formed.It is economic and practical, easy for assembly, again that the dynamic of the present invention is incubated simulation system 100
Renaturation is good, safe and effective.
" incubation system " of the present invention has high clinical value；Simultaneously because catheter correlation urinary tract infections, secreting
The incidences of disease such as the implant infection of urine system, calculus height and ascendant trend, therefore simulate bladder inner ring based on " incubation system " is somebody's turn to do
The bacterial biof iotalm series of studies and achievement in border also have high economic benefit and market prospects.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (9)
1. a kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system, it is characterised in that including：Storage of urine, peristaltic pump,
Box, urine collecting device and connecting tube are incubated, the storage of urine, peristaltic pump, is incubated between box and urine collecting device and is distinguished by the connecting tube
It is sequentially connected；Wherein, the lid for being incubated box and including box body and being connected with the box body, the both sides of the box body are opened respectively
Provided with inlet opening and fluid hole, the inlet opening is connected by the connecting tube with the outlet of the peristaltic pump, the fluid hole
It is connected by the connecting tube with the urine collecting device.
2. perfusion type bladder bacterial biof iotalm dynamic according to claim 1 is incubated simulation system, it is characterised in that described
Height of the inlet opening apart from the box body bottom is more than height of the fluid hole apart from the box body bottom.
3. perfusion type bladder bacterial biof iotalm dynamic according to claim 2 is incubated simulation system, it is characterised in that described
Being incubated box includes the inlet opening of two horizontally spaced settings and the fluid hole of two horizontally spaced settings.
4. perfusion type bladder bacterial biof iotalm dynamic according to claim 1 is incubated simulation system, it is characterised in that described
Be incubated the threading straight coupling that box also includes with the inlet opening and the fluid hole coordinating respectively, the threading straight coupling with
The connecting tube connection.
5. perfusion type bladder bacterial biof iotalm dynamic according to claim 1 is incubated simulation system, it is characterised in that described
Being incubated box also includes multiple demarcation strips, and direction of multiple demarcation strips along the inlet opening and the fluid hole line is arranged at
In the box body.
6. the perfusion type bladder bacterial biof iotalm dynamic according to claim any one of 1-5 is incubated simulation system, its feature
It is, the incubation box is immunohistochemical wet box.
7. perfusion type bladder bacterial biof iotalm dynamic according to claim 1 is incubated simulation system, it is characterised in that described
The outlet of storage of urine is connected by the connecting tube with the entrance of the peristaltic pump, and the outlet of the peristaltic pump passes through the connection
Manage and be connected with the entrance of the incubation box, the outlet for being incubated box is connected by the entrance of the connecting tube and the urine collecting device
Connect.
8. perfusion type bladder bacterial biof iotalm dynamic according to claim 1 is incubated simulation system, it is characterised in that described
Storage of urine and the urine collecting device are the glass triangle flask with sealing-plug, and the sealing-plug is connected with the connecting tube.
9. perfusion type bladder bacterial biof iotalm dynamic according to claim 1 is incubated simulation system, it is characterised in that described
Connecting tube is silicone tube.
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| CN109935147A (en) * | 2019-03-26 | 2019-06-25 | 长春大学 | A kind of bladder environment simulator |
| CN110164279A (en) * | 2019-06-21 | 2019-08-23 | 四川省人民医院 | A kind of bladder urine flow turbulence shear stress simulation system |
| CN110164279B (en) * | 2019-06-21 | 2020-06-09 | 四川省人民医院 | Bladder uroflow turbulent shear stress simulation system |
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