CN106701542A - Multifunctional culture device for bacteria biofilm - Google Patents
Multifunctional culture device for bacteria biofilm Download PDFInfo
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- CN106701542A CN106701542A CN201710174953.3A CN201710174953A CN106701542A CN 106701542 A CN106701542 A CN 106701542A CN 201710174953 A CN201710174953 A CN 201710174953A CN 106701542 A CN106701542 A CN 106701542A
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- culture
- biofilm
- tank
- disk
- peristaltic pump
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/14—Pressurized fluid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/12—Pulsatile flow
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a multifunctional culture device for a bacteria biofilm. The multifunctional culture device comprises a culture medium storage tank for accommodating a liquid culture medium, CO2 and air, a peristaltic pump assembly and a biofilm culture tank, wherein the culture medium storage tank is connected with the peristaltic pump assembly through a guide pipe I; the peristaltic pump assembly is connected with the biofilm culture tank through a guide pipe II; the biofilm culture tank is a barrel body with a sealing cover; the side wall of the bottom of the biofilm culture tank is connected with an output pipe for enabling sampling detection and discharging waste liquid; a disk for placing culture carriers is arranged in the biofilm culture tank; multiple groups of clamping slots for placing the culture carriers are formed in the disk. The multifunctional culture device for the bacteria biofilm is simple in structure, multifunctional and high in practicability; the multifunctional culture device is different from a conventional single biofilm culture device, and can supply sufficient experimental materials to lots of researches on the bacteria biofilm.
Description
Technical field
The present invention relates to germ culturing device technical field, and in particular to a kind of multi-functional culture dress of bacterial biofilm
Put.
Background technology
Biofilm is a kind of germs collect colony, is that bacterium sticks to abiotic or activity to adapt to living environment
Tissue surface, is coated in the mucus heterogeneity polymeric matrices of autologous generation, forms a kind of mode corresponding with planktonic bacteria
The colony of growth, is made up of thalline and Extracellular polymers.In biofilm state cell table shape or physiologic character all with
State of swimming is different, wherein being just the increase in the tolerance of the raw element of confrontation than more typical property.At present, many antibiotic are controlled
The reason for treating failure is all attributed to the formation of biofilm, and at least infection of 65-80% is considered as relevant with biofilm.
The life of people is more affected due to biofilm, it is necessary to increase the research dynamics to biofilm.This
Just a set of traditional single creature envelope culture apparatus is different from the urgent need to we set up.Single creature envelope culture apparatus,
I.e.：The biofilm under liquid environment or gaseous environment can only be cultivated, and can only once cultivate the biological quilt under a kind of material
Film.Harmfulness high in biofilm, in the urgent need to numerous studies in the case of, single culture apparatus and cumbersome culture step
It is rapid to bring very heavy work load to scientific research personnel, plenty of time and energy are wasted, lead to not quickly promote biofilm to grind
The development studied carefully.
The content of the invention
The purpose of the present invention is in order to solve the above technical problems, providing a kind of multi-functional culture dress of bacterial biofilm
Put, construction is terse, vdiverse in function, practical, is different from traditional single creature envelope culture apparatus, can be bacterium living beings
The numerous studies of envelope provide sufficient experiment material.
The present invention is in order to solve the above technical problems, the technical scheme for being provided is：A kind of bacterial biofilm it is multi-functional
Culture apparatus, including for accommodating fluid nutrient medium, CO2Nutrient media storage tank, peristaltic pump component and biological quilt with air
Membrance cuiture tank, is connected between the nutrient media storage tank and peristaltic pump component by conduit I, and peristaltic pump component is trained with biofilm
Connected by conduit II between foster tank, the biofilm culture tank is the cylinder with closure, biofilm culture tank
Bottom sidewall is connected with the efferent duct for sampling detection and discharge waste liquid, and biofilm culture tank is internally provided with to be trained for placement
The disk of carrier is supported, multigroup draw-in groove for placing culture carrier is provided with disk, draw-in groove is distributed uniformly and circumferentially
On disk.
As a kind of further optimization of the multi-functional culture apparatus of bacterial biofilm of the invention：Set on the disk
There are the six groups or eight groups draw-in grooves for placing culture carrier.
As a kind of further optimization of the multi-functional culture apparatus of bacterial biofilm of the invention：Every group of described draw-in groove
It is made up of edge groove and central channel two parts, and the opening of edge groove and central channel mutually corresponds to, and culture carrier is fastened on two
Between person.
As a kind of further optimization of the multi-functional culture apparatus of bacterial biofilm of the invention：The biofilm training
Support the through hole for having on the closure of tank and being available for conduit II to penetrate.
Beneficial effect
1st, the multi-functional culture apparatus of bacterial biofilm of the invention, construction is terse, vdiverse in function, practical, is different from
Traditional single creature envelope culture apparatus, can provide the experiment material of abundance for the numerous studies of bacterial biofilm；
2nd, the multi-functional culture apparatus of bacterial biofilm of the invention, can be continuous to nutrient media storage by peristaltic pump component
Tank is passed through gas culture medium, builds gaseous environment, and can continuously be passed through gas, builds the gaseous environment of flowing；Can also lead to
Cross peristaltic pump and be continuously passed through fluid nutrient medium to nutrient media storage tank, build the liquid environment of flowing, and can continuously be passed through liquid
Body, builds the liquid environment of flowing；By adjusting the input speed of peristaltic pump, gas or liquid are passed through with certain speed, fitted
Answer the fostering requirement of different bacterium；
3rd, the multi-functional culture apparatus of bacterial biofilm of the invention, is provided with multiple for placing culture carrier on disk
Draw-in groove, rectangle culture carrier increased the contact area of culture carrier and culture medium and can be while placing different materials
Culture carrier, so that under differentiating equivalent environment, different materials form the ability of bacterial biofilm.
Brief description of the drawings
Fig. 1 is the structural representation of multi-functional culture apparatus of the invention；
Fig. 2 is the structural representation of the disk of multi-functional culture apparatus of the invention；
Marked in figure：1st, nutrient media storage tank, 2, conduit I, 3, peristaltic pump component, 4, conduit II, 5, efferent duct, 6, biofilm
Culture tank, 7, culture carrier, 8, disk, 9, draw-in groove.
Specific embodiment
Below in conjunction with the accompanying drawings and preferred embodiment describes specific embodiment of the invention in detail.
As shown in the figure：A kind of multi-functional culture apparatus of bacterial biofilm, including for accommodating fluid nutrient medium, CO2
Nutrient media storage tank 1, peristaltic pump component 3 and biofilm culture tank 6 with air, the nutrient media storage tank 1 and wriggling
Connected by conduit I 2 between pump group part 3, connected by conduit II 4 between peristaltic pump component 3 and biofilm culture tank 6, institute
It is the cylinder with closure to state biofilm culture tank 6, has on the closure of biofilm culture tank 6 and is available for conduit II 4
The through hole for penetrating, the bottom sidewall of biofilm culture tank 6 is connected with the efferent duct 5 for sampling detection and discharge waste liquid, biological
Envelope culture tank 6 is internally provided with the disk 8 for placing culture carrier 7, and six groups or eight groups of use are provided with the disk 8
In the draw-in groove 9 for placing culture carrier 7, six groups or eight groups of draw-in grooves 9 are distributed uniformly and circumferentially on disk 8, every group of draw-in groove
Be made up of edge groove and central channel two parts, and edge groove and central channel opening mutually correspond to culture carrier is fastened on both
Between.
Application method of the invention：
1. prepare before operating：The seal of the device each several part is checked, various pieces is cleaned and sterilization treatment, assembled,
Open the closure of nutrient media storage tank.
2. culture carrier is placed：According to experiment purpose and demand, at most eight are placed in the disk with draw-in groove by identical
The culture carrier that material or different materials are made.
3. culture medium is added：It is sterilized to being added in nutrient media storage tank according to culture thalline and the demand of research
Liquid or gas culture medium, then close the closure of nutrient media storage tank.
4. strain is inoculated with：Aseptically, part is provided by power（Peristaltic pump）Add in biofilm culture tank
After entering the culture medium by sterilizing, inoculation needs the bacteria culture of culture and covers closure.
5. cultivate：The culture of bacterial biofilm is carried out, in incubation, detection can be sampled through efferent duct, work as life
When culture bacteria concentration reaches standard requirement in thing envelope culture tank, it is input into biofilm culture tank again by peristaltic pump component
Culture medium, after making culture bacteria concentration reach decline, continues to cultivate, and is reached again until cultivating bacteria concentration in biofilm culture tank
Standard requirement, is so repeated a number of times, and the culture medium in biofilm culture tank may be at intermittent flow in this process
Dynamic state, forms the environment of flowing.
6. sterilizing is preserved：Culture biofilm and using finishing after, discarded culture medium is discharged by delivery outlet, it is then right
Package unit splits preservation again after carrying out sterilization treatment.
Multi-functional culture apparatus of the invention can be passed through gas culture by the way that peristaltic pump is continuous to nutrient media storage part
Base, builds gaseous environment, and can continuously be passed through gas, builds the gaseous environment of flowing；Can be continuous to training by peristaltic pump
Support base storage part and be passed through fluid nutrient medium, build the liquid environment of flowing, and can continuously be passed through liquid, build the liquid of flowing
Body environment；Gas or liquid can be passed through with certain speed by adjusting the input speed of peristaltic pump, adapt to different bacterium
Fostering requirement；Six pieces are placed in disk tongue or eight pieces of rectangle culture carriers increased contact of the culture carrier with culture medium
Area and the culture carrier of different materials can be simultaneously placed, so that under differentiating equivalent environment, different materials form bacterium life
The ability of thing envelope；Present invention construction is terse, vdiverse in function, practical, can accelerate research with save resources, reduces cost
Progress.
The above, is only presently preferred embodiments of the present invention, and any formal limitation is not made to the present invention, though
So the present invention is disclosed above with preferred embodiment, but is not limited to the present invention, any to be familiar with this professional technology people
Member, without departing from the scope of the present invention, when making a little change or modification using the technology contents of the disclosure above
It is the Equivalent embodiments of equivalent variations, as long as being without departing from technical solution of the present invention content, according to technical spirit pair of the invention
Any simple modification, equivalent variations and modification that above example is made, still fall within the range of technical solution of the present invention.
Claims (4)
1. the multi-functional culture apparatus of a kind of bacterial biofilm, it is characterised in that：Including for accommodating fluid nutrient medium, CO2With
The nutrient media storage tank of air（1）, peristaltic pump component（3）And biofilm culture tank（6）, the nutrient media storage tank（1）
With peristaltic pump component（3）Between pass through conduit I（2）Connection, peristaltic pump component（3）With biofilm culture tank（6）Between pass through
Conduit II（4）Connection, the biofilm culture tank（6）It is the cylinder with closure, biofilm culture tank（6）Bottom
Side wall is connected with the efferent duct for sampling detection and discharge waste liquid（5）, biofilm culture tank（6）It is internally provided with for placing
Culture carrier（7）Disk（8）, disk（8）On be provided with it is multigroup for placing culture carrier（7）Draw-in groove（9）, draw-in groove（9）
It is distributed uniformly and circumferentially in disk（8）On.
2. the multi-functional culture apparatus of a kind of bacterial biofilm as claimed in claim 1, it is characterised in that：The disk
（8）On be provided with six groups or eight groups for placing culture carrier（7）Draw-in groove（9）.
3. the multi-functional culture apparatus of a kind of bacterial biofilm as claimed in claim 1, it is characterised in that：Described every group
Draw-in groove（9）It is made up of edge groove and central channel two parts, and the opening of edge groove and central channel mutually corresponds to, and makes culture carrier
（7）It is fastened between the two.
4. the multi-functional culture apparatus of a kind of bacterial biofilm as claimed in claim 1, it is characterised in that：The biological quilt
Membrance cuiture tank（6）Closure on have be available for conduit II（4）The through hole for penetrating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710174953.3A CN106701542A (en) | 2017-03-22 | 2017-03-22 | Multifunctional culture device for bacteria biofilm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710174953.3A CN106701542A (en) | 2017-03-22 | 2017-03-22 | Multifunctional culture device for bacteria biofilm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106701542A true CN106701542A (en) | 2017-05-24 |
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ID=58887315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710174953.3A Pending CN106701542A (en) | 2017-03-22 | 2017-03-22 | Multifunctional culture device for bacteria biofilm |
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| CN (1) | CN106701542A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418891A (en) * | 2017-09-20 | 2017-12-01 | 四川省人民医院 | A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system |
| CN112522093A (en) * | 2020-12-01 | 2021-03-19 | 深圳市第二人民医院(深圳市转化医学研究院) | Air flue microenvironment simulation device formed by bacterial biofilm |
| CN113087068A (en) * | 2021-04-01 | 2021-07-09 | 广东工业大学 | Photocatalytic oxidation device for in-situ culture or/and killing of biological membrane and use method and application thereof |
Citations (5)
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| WO1999002646A1 (en) * | 1997-07-11 | 1999-01-21 | Corning Incorporated | Self contained cell growth system |
| CN2761164Y (en) * | 2004-12-30 | 2006-03-01 | 中国科学院电工研究所 | Biological reactor |
| US20070275452A1 (en) * | 2006-05-26 | 2007-11-29 | Sharp Kabushiki Kaisha | Biological reaction method and bioreactor |
| CN204569901U (en) * | 2015-04-16 | 2015-08-19 | 洛阳师范学院 | A kind of liquid culturing apparatus of bacterial biofilm |
| CN206751800U (en) * | 2017-03-22 | 2017-12-15 | 洛阳师范学院 | A kind of multi-functional culture apparatus of bacterial biofilm |
-
2017
- 2017-03-22 CN CN201710174953.3A patent/CN106701542A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999002646A1 (en) * | 1997-07-11 | 1999-01-21 | Corning Incorporated | Self contained cell growth system |
| CN2761164Y (en) * | 2004-12-30 | 2006-03-01 | 中国科学院电工研究所 | Biological reactor |
| US20070275452A1 (en) * | 2006-05-26 | 2007-11-29 | Sharp Kabushiki Kaisha | Biological reaction method and bioreactor |
| CN204569901U (en) * | 2015-04-16 | 2015-08-19 | 洛阳师范学院 | A kind of liquid culturing apparatus of bacterial biofilm |
| CN206751800U (en) * | 2017-03-22 | 2017-12-15 | 洛阳师范学院 | A kind of multi-functional culture apparatus of bacterial biofilm |
Non-Patent Citations (1)
| Title |
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| 四川华西口腔医院编: "中国口腔医学年鉴-2003卷", 四川科学技术出版社, pages: 26 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418891A (en) * | 2017-09-20 | 2017-12-01 | 四川省人民医院 | A kind of perfusion type bladder bacterial biof iotalm dynamic is incubated simulation system |
| CN112522093A (en) * | 2020-12-01 | 2021-03-19 | 深圳市第二人民医院(深圳市转化医学研究院) | Air flue microenvironment simulation device formed by bacterial biofilm |
| CN113087068A (en) * | 2021-04-01 | 2021-07-09 | 广东工业大学 | Photocatalytic oxidation device for in-situ culture or/and killing of biological membrane and use method and application thereof |
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