CN105670914A - Petri dish capable of continuously supplementing impact factors - Google Patents

Abstract

A petri dish capable of continuously replenishing influencing factors is composed of three parts: the bottom of the petri dish, the lid of the dish and a partition, the partition is a sand core filter plate, installed in the bottom of the petri dish, and separates the inner cavity of the bottom of the petri dish It is two independent spaces: the upper microbial culture space and the lower impact factor continuous supplementary space. The invention has a simple structure, can continuously supplement the influencing factors, realizes the constant concentration of the setting influencing factors in the process of microbial cultivation, and is not polluted by exogenous microorganisms, and is especially suitable for treating unstable or easily decomposed influencing factors at a constant concentration. Observations of microbial growth effects.

Description

A petri dish that can continuously replenish impact factors

technical field

The invention belongs to a microorganism cultivation device, in particular to a petri dish capable of continuously supplementing influencing factors.

Background technique

The existing microbial solid culture usually uses petri dishes, but this kind of petri dishes cannot supplement nutrients or other influencing factors during the microbial culture process. As the culture time continues, nutrients or other influencing factors in the medium The concentration of the concentration will gradually decrease, and it is impossible to observe the influence of specific nutrients or other influencing factors on the cultured microorganisms at a constant concentration. If the petri dish is opened to supplement nutrients or other influencing factors, the operation is cumbersome, and the microbial cultivation space is easily exposed to external sources. Microbial contamination, therefore, there is an urgent need to develop a new type of petri dish that can continuously supplement impact factors without being polluted by exogenous microorganisms.

Contents of the invention

The technical problem to be solved by the present invention is to solve the above-mentioned problems in the prior art, and provide a culture dish that can continuously supplement the influencing factors, so as to realize the constant concentration of the setting influencing factors in the microbial culture process, and not be affected by exogenous microorganisms. pollute.

The technical solution adopted in the present invention is: a petri dish that can continuously supplement the influencing factors, which consists of three parts: the bottom of the petri dish, the lid of the dish and a partition, the partition is a sand core filter plate, installed in the bottom of the petri dish , which divides the inner cavity of the bottom of the petri dish into two independent spaces: the upper space for microbial cultivation and the lower space for continuous replenishment of impact factors.

In the above technical solution, the continuous supplementary space of the lower impact factor is provided with an inlet of the mixed fluid containing the influence factor, an outlet of the mixed fluid containing the influence factor and an overflow port, the inner diameter of the inlet is larger than the inner diameter of the outlet, but the cross-sectional area of the inlet is smaller than the cross-sectional area of the outlet and the cross-sectional area of the overflow port, at the same time, the inlet position is lower than the outlet position, and the upper edge of the inner wall of the overflow port is flush with the lower part of the sand core filter plate.

In the above technical scheme, the bottom of the petri dish is 2-3 times higher than the bottom of the corresponding petri dish.

In the above technical solution, the partition is installed in the bottom of the petri dish at a height of 15 mm from the bottom, and the outer diameter of the partition is the same as the inner diameter of the bottom of the petri dish.

In the above technical solution, the separator is a sand core filter plate code-named G6, with a thickness of 2 mm and a pore diameter of <1.5 μm.

working principle:

The culture dish involved in the present invention is composed of three parts: the bottom of the culture dish, the lid of the dish, and the partition plate made of sand core filter plate material. The bottom of the culture dish is separated into two independent spaces by the partition board. The upper space is similar to that of a common petri dish. It is used for normal microbial culture, and the lower space is a continuous supplementary space for microbial culture influencing factors. There is one outlet, one inlet and one overflow respectively, and the influencing factor can be imported from the inlet and exported from the outlet along with the fluid such as water or air.

It is required that the inner diameter of the inlet is larger than the inner diameter of the outlet, the cross-sectional area of the inlet is smaller than the sum of the cross-sectional area of the outlet and the overflow, the position of the inlet is lower than that of the outlet, and the upper edge of the inner wall of the overflow is flush with the lower part of the partition to ensure that the fluid is always tightly connected to the partition Contact, the influence factor reaches the surface of the medium through the partition and the solid medium under the action of capillary force, so as to realize the constant concentration of the set influence factor during the microbial culture process.

Advantages and effects:

One is to realize continuous replenishment of microbial growth influencing factors when using petri dishes for solid culture; second, it can prevent the microbial culture space from being polluted by exogenous microorganisms during the continuous replenishment of influencing factors.

The invention has a simple structure, and is particularly suitable for the observation of the effect on the growth of microorganisms at a constant concentration of factors that are unstable in nature or easily decomposed by microorganisms.

Description of drawings:

Fig. 1 is a structural representation of the present invention;

Fig. 2 is a top view of the structure of the present invention.

Notes on the attached drawings: 1—the bottom of the culture dish, 2—the lid of the dish, 3—the partition, 4—the inlet of the mixed fluid with influencing factors,

5—exit of mixed fluid with influence factor, 6—overflow port.

detailed description:

Referring to Fig. 1, Fig. 2, the petri dish of the present invention, which can continuously replenish influencing factors, is composed of three parts: the bottom of the petri dish, the dish cover and the partition, and the partition is a sand core filter plate, which is installed in the bottom of the petri dish , divide the inner cavity of the bottom of the culture dish into two independent spaces: the upper microbial culture space and the lower impact factor continuous supplementary space, the lower impact factor continuous supplementary space is provided with the mixed fluid inlet containing the impact factor and the mixed fluid outlet with the impact factor And the overflow port, the inner diameter of the inlet is larger than the inner diameter of the outlet, but the cross-sectional area of the inlet is smaller than the sum of the cross-sectional area of the outlet and the cross-sectional area of the overflow port. The lower part of the plate is flush, and the bottom of the petri dish is 2-3 times higher than the bottom of the corresponding petri dish. The partition is installed at the bottom of the petri dish at a height of 15 mm from the bottom, and the outer diameter of the partition is the same as that of the culture dish. The inner diameter of the bottom of the dish is the same, and the separator is a sand core filter plate code-named G6, with a thickness of 2 mm and a pore diameter of <1.5 μm.

Claims (4)

1. A culture dish that can continuously replenish influencing factors is characterized in that: it is made up of three parts: at the bottom of the culture dish, a dish cover and a partition, and the partition is a sand core filter plate, which is installed in the bottom of the culture dish. The inner cavity at the bottom of the culture dish is divided into two independent spaces: the upper space for microbial cultivation and the lower space for continuous replenishment of impact factors. 2. The petri dish capable of continuously replenishing influencing factors according to claim 1, characterized in that: the space for continuously supplementing the lower influencing factors is provided with an inlet of mixed fluid containing influencing factors, an outlet of mixed fluid containing influencing factors and an overflow port , the inner diameter of the inlet is greater than the inner diameter of the outlet, but the cross-sectional area of the inlet is smaller than the sum of the cross-sectional area of the outlet and the cross-sectional area of the overflow. . 3. The petri dish capable of continuously replenishing influencing factors according to claim 1, characterized in that: the bottom of the petri dish is 2-3 times higher than the bottom of the corresponding petri dish; At the height of 15mm from the bottom of the petri dish, the outer diameter of the partition is the same as the inner diameter of the bottom of the petri dish. 4. The petri dish capable of continuously replenishing influencing factors according to claim 1, characterized in that: the separator is a sand core filter plate code-named G6, with a thickness of 2 mm and a pore diameter of <1.5 μm.