CN215774996U

CN215774996U - Culture dish for in-vitro preservation of schizocysticercus

Info

Publication number: CN215774996U
Application number: CN202122369893.5U
Authority: CN
Inventors: 刘伟; 李芬; 龚腾芳; 贺峻琳; 陈叔玉; 李文超; 谢馨锐
Original assignee: Hunan Agricultural University
Current assignee: Hunan Agricultural University
Priority date: 2021-09-28
Filing date: 2021-09-28
Publication date: 2022-02-11
Anticipated expiration: 2031-09-28

Abstract

The utility model discloses a culture dish for external preservation of lacerated miracidiums, which comprises a culture dish body and a culture dish cover covering the culture dish body, and is characterized by further comprising a light-shading cover, wherein one end of the light-shading cover is hinged with one side of the culture dish body, and the other end of the light-shading cover is connected with the other side of the culture dish body through a buckle; the light-shading cover and the culture dish body are both made of light-tight materials; the culture dish cover is made of a light-transmitting material. The utility model has simple structure and convenient use, and can provide a more stable environment for the in-vitro culture and storage of the sparganosis so as to prolong the in-vitro survival time of the sparganosis.

Description

Culture dish for in-vitro preservation of schizocysticercus

Technical Field

The utility model relates to a culture dish, in particular to a culture dish for in-vitro preservation of sparganum.

Background

The metacercaria is named as the metacercaria mansoni, is long and white, has different lengths of 0.3-105.0 cm and has strong stretching capacity. The head of the lacerated larva expands, the center of the lacerated larva has an obvious recess, the larva is not segmented but has irregular transverse folds, and the rear end of the larva is mostly in a shape of a blunt circle.

The human infected larva of the. After being eaten by people, the sparganum is most commonly parasitized in tissues and organs such as subcutaneous tissues, muscles, liver, lung, brain and the like of human bodies, and migrates in all tissues and organs of the organisms to cause the sparganosis, so as to form the fascicular or space occupying lesion. Clinical symptoms caused by different infected parts of the sparganosis are different, specific clinical manifestations are lacked, and misdiagnosis and mistreatment are common. Researches on the occurrence mechanism, the immune characteristics, the serodiagnosis, the clinical treatment and the like of the sparganosis all require stable animal models infected by the sparganosis and a large amount of standardized species resources with stable biological properties. However, the now experimental sparganosis mainly collected from naturally infected second intermediate host frog or tadpole, and cannot obtain live larva at any time due to seasonal factors, so that the sparganosis needs to be preserved in vitro for a period of time for convenient observation and detection.

SUMMERY OF THE UTILITY MODEL

In the process of researching the coenuruses, the coenuruses are generally required to be stored in vitro for a period of time to keep the coenuruses fresh and alive, so as to be convenient for observation or detection; at present, the coenuruses are generally stored in vitro in a short time by adopting a glass culture dish, but the glass culture dish has single function and is easy to transmit light, and the coenuruses are not beneficial to survival under strong light; in addition, in the process of storage and culture, because the coenuruses are required to be frequently transferred to replace the culture solution, the survival rate of the coenuruses is further reduced, and the glass culture dish is difficult to carry out in-vitro long-time culture and storage on the living bodies of the coenuruses; aiming at the problems, the utility model provides a culture dish for in-vitro conservation of the sparganosis, which has simple structure and convenient use, and can provide a more stable environment for in-vitro culture and storage of the sparganosis so as to prolong the in-vitro survival time of the sparganosis.

The utility model is realized by the following technical scheme.

The culture dish for the external preservation of the lacerated miracidium comprises a culture dish body and a culture dish cover covering the culture dish body, and is characterized by further comprising a light-shading cover, wherein one end of the light-shading cover is hinged with one side of the culture dish body, and the other end of the light-shading cover is connected with the other side of the culture dish body through a buckle; the light-shading cover and the culture dish body are both made of light-tight materials; the culture dish cover is made of a light-transmitting material.

As a specific technical scheme, one end of the upper surface of the light-proof cover, which is close to the buckle, is provided with a light-proof cover bracket in a foldable manner; when the light-proof cover is turned over and opened in parallel, one side of the light-proof cover is fixed with the culture dish body through the hinged end of the light-proof cover, and the other end of the light-proof cover is supported through the light-proof cover support, so that the light-proof cover is kept horizontal.

As a specific technical scheme, a visual window is arranged on the side surface of the culture dish body.

As a specific technical scheme, the bottom surface of the light-resistant cover and the surface of the visual window are both provided with scale marks.

As a specific technical scheme, a liquid discharging opening is formed in the bottom of the side face of the culture dish body; the water inlet end of the liquid discharging port is provided with a filter screen, and the water outlet end of the liquid discharging port is provided with a liquid discharging sealing plug.

As a specific technical scheme, the culture dish cover and the culture dish body are connected and covered through threads.

As a specific technical scheme, two sides of the upper surface of the culture dish cover are symmetrically provided with pinching handles; and a handle pinching groove matched with the handle pinching is arranged on the light-shading cover.

As a specific technical scheme, a liquid feeding port and a liquid feeding sealing plug matched with the liquid feeding port are arranged on the culture dish cover.

The utility model has the beneficial effects that:

1) the culture dish is provided with the light-proof cover, and the light-proof cover and the culture dish body are both made of light-tight materials, so that the phenomenon that the survival rate of the sparganosis is influenced due to the fact that strong light irradiates the sparganosis for a long time can be avoided.

2) In the culture dish, the bottom of the side surface of the culture dish body is provided with the liquid discharging port, the water inlet end of the liquid discharging port is provided with the filter screen and the liquid discharging sealing plug, the culture dish cover is provided with the liquid feeding port and the liquid feeding sealing plug, when the culture solution needs to be replaced after the sporotrichiasis is cultured for a period of time, the liquid discharging sealing plug can be opened, the culture solution in the culture dish is directly discharged, and the filter screen can prevent the sporotrichiasis from being discharged; when the culture solution in the culture dish is discharged partially or completely, the culture solution can be added through the liquid adding port, so that the coenurosis does not need to be frequently transferred, and the survival rate of the coenurosis can be improved.

3) When the culture dish is used, when the light-resistant cover is turned over and opened in parallel, the coenuruses can be clamped and placed on the light-resistant cover, and scales are arranged on the bottom surface of the light-resistant cover, so that the body shapes and the lengths of the coenuruses can be observed and detected conveniently.

Drawings

FIG. 1 is a front view of a culture dish according to the utility model;

FIG. 2 is a top view of a culture dish according to the utility model;

FIG. 3 is a front view of the light-shielding lid of the culture dish of the present invention when open;

FIG. 4 is a top view of the light-resistant cover of the culture dish of the present invention when open;

FIG. 5 is a schematic cross-sectional view taken along line A-A of FIG. 2;

the meanings of the marks in the above figures are as follows: 1-culture dish body, 101-transparent visual window, 102-liquid discharging port, 103-filter screen, 104-liquid discharging sealing plug, 2-culture dish cover, 201-handle, 202-liquid charging port, 203-liquid charging sealing plug, 3-light-proof cover, 301-light-proof cover bracket, 302-handle-holding groove and 4-graduation line.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Examples

The culture dish for in-vitro preservation of the lacerated larvae refers to the figures 1 to 5, and comprises a culture dish body 1, a culture dish cover 2 covering the culture dish body 1, and a light-proof cover 3; the light-shading cover 3 is hinged to one side of the culture dish body 1 at one end and connected to the other side of the culture dish body 1 at the other end through a buckle; the light-proof cover 3 and the culture dish body 1 are both made of light-proof materials, such as light-proof plastics, metals and the like; the culture dish cover 2 is made of a light-transmitting material, such as transparent glass, transparent plastic and the like.

Further, in a preferred embodiment, referring to fig. 3 and 4, the light-shielding cover bracket 301 is foldably provided at one end of the upper surface of the light-shielding cover 3, which is close to the buckle; when the light-proof cover 3 is turned over and opened in parallel, one side of the light-proof cover 3 is fixed with the culture dish body 1 through the hinged end of the light-proof cover, and the other end of the light-proof cover is supported through the light-proof cover bracket 301, so that the light-proof cover 3 is kept horizontal; based on the structure, the light-resistant cover 3 can be turned over and opened at any time, so that the light-resistant cover 3 is kept horizontal to be used as a temporary platform for observing the sparganum, then the culture dish cover 2 can be opened, and the sparganum is clamped and placed on the light-resistant cover 3, so that the length and the form change of the sparganum in the growth process can be observed at any time.

In order to facilitate the observation of the shapes of the sparganium from the side or to grasp the volume and turbidity of the culture solution in time, further, in a preferred embodiment, referring to fig. 1 and 3, the side of the culture dish body 1 is provided with a visual window 101.

Further, in a preferred embodiment, referring to fig. 1 and 4, the bottom surface of the light-shielding cover 3 and the surface of the visible window 101 are provided with scale marks 4.

In order to facilitate the discharge of the culture solution in the culture dish and the replacement of the culture solution in time, and maintain the survival conditions of the sparganosis, further, in a preferred embodiment, referring to fig. 1 and fig. 5, the bottom of the side surface of the culture dish body 1 is provided with a liquid discharging port 102; a filter screen 103 is arranged at the water inlet end of the liquid discharging port 102, and a liquid discharging sealing plug 104 is arranged at the water outlet end of the liquid discharging port 102.

In order to make the culture dish cover 2 and the culture dish body 1 close, further, in a preferred embodiment, the culture dish cover 2 and the culture dish body 1 are connected and closed through threads.

Further, in a preferred embodiment, referring to fig. 3 to 5, two sides of the upper surface of the culture dish cover 2 are symmetrically provided with handles 201; the light-resistant cover 3 is provided with a knob groove 302 matched with the knob 201.

In order to facilitate the culture solution to be added into the culture dish, further, in a preferred embodiment, referring to fig. 3 and 5, a feeding port 202 and a feeding sealing plug 203 matched with the feeding port 202 are arranged on the culture dish cover 2.

Claims

1. The culture dish for the external preservation of the lacerated miracidium comprises a culture dish body (1) and a culture dish cover (2) covering the culture dish body (1), and is characterized by further comprising a light-shading cover (3), wherein one end of the light-shading cover is hinged with one side of the culture dish body (1), and the other end of the light-shading cover is connected with the other side of the culture dish body (1) through a buckle; the light-proof cover (3) and the culture dish body (1) are both made of light-proof materials; the culture dish cover (2) is made of a light-transmitting material.

2. The culture dish for the in-vitro preservation of the sparganum according to claim 1, characterized in that the upper surface of the light-proof cover (3) is provided with a light-proof cover bracket (301) at one end close to the buckle in a foldable way; when the light-proof cover (3) is turned over and opened in parallel, one side of the light-proof cover (3) is fixed with the culture dish body (1) through the hinged end of the light-proof cover, and the other end of the light-proof cover is supported through the light-proof cover bracket (301), so that the light-proof cover (3) is kept horizontal.

3. A culture dish for the extracorporeal preservation of sparganosis according to claim 1, wherein the side of the culture dish body (1) is provided with a transparent visual window (101).

4. A culture dish for the in vitro preservation of sparganosis as in claim 3, characterized in that the bottom surface of the light-resistant cover (3) and the surface of the transparent visual window (101) are provided with scale marks (4).

5. The culture dish for the in vitro preservation of the sparganosis according to claim 1, characterized in that the bottom of the side surface of the culture dish body (1) is provided with a liquid outlet (102); a filter screen (103) is arranged at the water inlet end of the liquid discharging port (102), and a liquid discharging sealing plug (104) is arranged at the water outlet end of the liquid discharging port (102).

6. The culture dish for the extracorporeal preservation of sparganosis according to claim 1, wherein the culture dish lid (2) is covered with the culture dish body (1) through a threaded connection.

7. The culture dish for the in-vitro preservation of the sparganosis according to claim 1, characterized in that the two sides of the upper surface of the culture dish cover (2) are symmetrically provided with a knob (201); and a handle pinching groove (302) matched with the handle pinching (201) is formed in the light-proof cover (3).

8. The culture dish for the in-vitro preservation of the sparganosis according to any one of claims 1 to 7, characterized in that a liquid adding opening (202) and a liquid adding sealing plug (203) matched with the liquid adding opening (202) are arranged on the culture dish cover (2).