CN204305886U - Diamond-back moth gets ovum and egg hatching observation device - Google Patents
Diamond-back moth gets ovum and egg hatching observation device Download PDFInfo
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- CN204305886U CN204305886U CN201420704824.2U CN201420704824U CN204305886U CN 204305886 U CN204305886 U CN 204305886U CN 201420704824 U CN201420704824 U CN 201420704824U CN 204305886 U CN204305886 U CN 204305886U
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Abstract
The utility model relates to a kind of diamond-back moth scientific research technical field, and specifically a kind of diamond-back moth gets ovum and egg hatching observation device.A kind of diamond-back moth gets ovum and egg hatching observation device, comprise and get egg apparatus and see egg apparatus, described get that egg apparatus comprises tubbiness get egg apparatus main body, get egg apparatus main body the first half and be provided with air-vent I, get egg apparatus body top edge bundle and have an aperture, aperture establishes the sliver being inserted with and dipping in glucose solution, the bung place of getting egg apparatus main body is provided with double-layer plastic film, double-layer plastic film is tightened by rubber band and is being got in egg apparatus main body, double-layer plastic film is provided with some air-vent II, and double-layer plastic film interlayer place is placed with cabbage leaves; Described sight egg apparatus is the barreled structure that diameter is identical with getting egg apparatus main body, sees egg apparatus bung place and is provided with double-layer plastic film from getting egg apparatus and taking off.
Description
Technical field
The utility model relates to a kind of diamond-back moth scientific research technical field, and specifically a kind of diamond-back moth gets ovum and egg hatching observation device.
Background technology
Diamond-back moth (Plutella xylostella) belongs to Lepidoptera diamond-back moth section, be a kind of global, to cause heavy losses, fertility are strong, pesticide resistance is strong brassicaceous vegetable, oil plant insect (the outstanding people's livelihood etc. to the mankind, 2007), the loss that the whole world causes because of diamond-back moth every year and expenses for prevention and control reach 1,000,000,000 dollars (what covolume etc., 2000).Under research different condition, the development duration of Eggs of Diamondback Moth, incubation rate are the important steps disclosing diamond-back moth ecological rule, are one of the important foundation work of formulating diamond-back moth prediction model, control strategy.
Eggs of Diamondback Moth is oval, and long 0.5 millimeter, wide 0.3 millimeter, milky during primiparity, rear thin out yellow green will be black (the outstanding people's livelihood etc., 2007) when will hatch.It is the shortest 2 days that egg development goes through the phase, the longest 51 days (Ke Lidao etc., 1979).
First number just number of eggs laid, then every number of days residue ovum number, calculates and hatches number day by day, calculate ovum accordingly and go through phase and incubation rate (Ma Chunsen etc., 1993).Test generally needs to arrange 3-5 process, and each process needs 50-100 grain ovum.Because ovum grain is little, color is close with attachment, and according to existing research method, every number of days ovum need use 25-30 minute, and easily leaks number, tuple, increases experimental error.Quick and precisely counting egg hatching number every day can save the time and efforts of researcher preciousness, reduces experimental error.
The technical scheme (Ma Chunsen etc., 1993) of prior art one: be placed in the glass tube of 4 × 13cm of one end block by a pair male and female moth, produces the ovum grain counting on tube wall by adult, daily check residue ovum number, calculates and hatch number day by day.
Prior art one Problems existing: 1. ovum dispersion, count range is large.Ovum is randomly dispersed in inboard wall of test tube everywhere.2. experimental error is large.On test tube, not easy-to-use thinner carbon pen makes marks on ovum position, leakiness number, tuple.3. need the time many.Can only count and not hatch ovum number, calculate hatch number day by day with this, directly can not count and hatch number day by day, hatching peak previous work amount is large.
The technical scheme of prior art two (but foundation etc., 1995): after adult eclosion, match raising immediately, be provided with 20% hydromel for food, and put into the filter paper being impregnated with cabbage leaves juice in laying eggs cage, lay eggs to attract female worm, foster worm ware is placed in there being the filter paper of fresh ovum, often process more than 100, put into climatic cabinate, observe hatching progress and the survival condition of ovum every day.
Prior art two Problems existing: except the problem of prior art one, also has following problem: the filter paper being impregnated with cabbage leaves juice is opaque, and color is close with ovum, not easy to identify, counting.
The technical scheme (Ke Lidao etc., 1979) of prior art three: after adult eclosion the vial of 500 milliliters is put in male and female pairing the same day, hangs one, fresh vegetable leaf in bottle, for Adult worms producting eggs, ovum is placed in the culture dish of 13 centimetres, by means of moistening filter paper moisturizing, morning and afternoon every day each observed and recorded once.
Prior art three Problems existing: outside the problem apart from technology one and two, also has following problem: ovum mainly produces at dish leaf back, but also has and produce in front, because dish leaf is opaque, easily damage when leafing through counting the ovum erasing opposite, error count is hatching ovum, strengthens experimental error.
Summary of the invention
The utility model, in order to solve the problem, provides a kind of diamond-back moth to get ovum and egg hatching observation device.
The utility model takes following technical scheme: a kind of diamond-back moth gets ovum and egg hatching observation device, comprise and get egg apparatus and see egg apparatus, described get that egg apparatus comprises tubbiness get egg apparatus main body, getting egg apparatus the latter half is funnel-form, bottom is provided with seal cover, get egg apparatus main body the first half and be provided with air-vent I, get egg apparatus body top edge bundle and have an aperture, aperture establishes the sliver being inserted with and dipping in glucose solution, the bung place of getting egg apparatus main body is provided with double-layer plastic film, double-layer plastic film is tightened by rubber band and is being got in egg apparatus main body, double-layer plastic film is provided with some air-vent II, double-layer plastic film interlayer place is placed with cabbage leaves, described sight egg apparatus is the barreled structure that diameter is identical with getting egg apparatus main body, sees egg apparatus bung place and is provided with double-layer plastic film from getting egg apparatus and taking off.
Described egg apparatus main body the first half of getting is provided with the air-vent I distributed by 10 millimeters × 10 millimeters, small pitch gets egg apparatus body top edge 3-4 centimetre, air-vent II on double-layer plastic film is by 0.5 cm x 0.5 centimetre distribution, and the size of cabbage leaves is 0.5 cm x 0.5 centimetre.
Compared with prior art, the beneficial effect that brings of technical solutions of the utility model is as follows:
(1), significantly shorten observation gate time.The test of an observation 500 ovum, counting conventional method needs more than 50 minutes (in order to accurately, sometimes several more than 2 times) first, needs 15 minutes (number is once) by this method; After hatching starts, this method will need about 5 minutes average tomorrow, and save 20 minutes than conventional method, the whole test period, can save time 70-150 minute altogether.
(2), count more accurately, decrease experimental error.One flange ovum is number number on one side, avoids repeat number and leaks number.As midway has special thing to interrupt, also by the numeral meter of number on paper, then number again can be finished afterwards, need not whole tuple.
(3), identification is easier.Egg membrane water white transparency, with the naked eye can know and distinguish ovum grain, scrutinize with the magnifying glass of 20 times, chorion remaining after can seeing egg hatching, this ovum can be confirmed really hatch, the ovum of blackening can be clear that end to end in ovum, that body is folding larva body, represents that this ovum will be hatched soon, sometimes can see the ovum having stretched out half body and hatched.
(4) damage to ovum, is avoided.Ovum towards internal fixtion on Plastic Drum, drawing a circle severally makes marks all outside, can not touch ovum.Plastic Drum is high 10 centimetres, carries, deposits, observes and all can not encounter ovum.
Accompanying drawing explanation
Fig. 1 gets egg apparatus front view;
Fig. 2 gets egg apparatus vertical view;
Fig. 3 sees egg apparatus front view;
In figure, 1-gets egg apparatus main body, 2-double-layer plastic film, 3-cabbage leaves, 4-air-vent I, 5-sliver, 6-aperture, and 7-air-vent II, 8-see egg apparatus.
Embodiment
A kind of diamond-back moth gets ovum and egg hatching observation device, comprise and get egg apparatus and see egg apparatus, described get that egg apparatus comprises tubbiness get egg apparatus main body 1, getting egg apparatus the latter half is funnel-form, bottom is provided with seal cover, get egg apparatus main body 1 the first half and be provided with air-vent I4, get egg apparatus main body 1 upper edge bundle and have an aperture 6, aperture 6 establishes the sliver 5 being inserted with and dipping in glucose solution, the bung place of getting egg apparatus main body 1 is provided with double-layer plastic film 2, double-layer plastic film 2 is tightened by rubber band and is being got in egg apparatus main body, double-layer plastic film 2 is provided with some air-vent II7, double-layer plastic film 2 interlayer place is placed with cabbage leaves 3, described sight egg apparatus 8 is barreled structures that diameter is identical with getting egg apparatus main body 1, sees egg apparatus 8 bung place and is provided with double-layer plastic film 2 from getting egg apparatus 1 and taking off.
Described egg apparatus main body 1 the first half of getting is provided with the air-vent I4 distributed by 10 millimeters × 10 millimeters, aperture 6 is apart from getting egg apparatus main body 1 top edge 3-4 centimetre, air-vent II7 on double-layer plastic film 2 is by 0.5 cm x 0.5 centimetre distribution, and the size of cabbage leaves 3 is 0.5 cm x 0.5 centimetre.
1, material: 250mm mineral water bottle 1,3, rubber band, polyethylene plastic film 2 pieces (14 × 14cm, thick 0.005 millimeter), 1, sliver (diameter 1 millimeter, long 3 centimetres), 20% glucose solution is some, fresh cabbage leaves 1.
2, instrument: one, scissors, special thin sewing-needle one, tweezers one, special thin carbon pen one.
3, manufacturing process: apart from bottleneck 10 centimeters in the middle part of mineral water bottle, be cut into upper and lower two parts with scissors is parallel, is respectively and gets egg apparatus main body, sees egg apparatus, and the latter half cuts off the end becomes a high drum of about 10 centimetres.Clip is carefully pruned, is placed on smooth concrete floor and polishes, make the level and smooth impulse-free robustness of clip, be unlikely to during use hinder hand or pierce through plastic foil.
The first half is according to the special finedraw clothing pinprick hole of specification of 10 millimeters × 10 millimeters, for ventilation, with tweezers point at the aperture pricking a diameter 0.5 millimeter apart from clip edge 3-4 centimeters, sliver dips in the glucose solution of 25% along this hole insertion confession diamond-back moth imago feeding, by one piece of plastics membrane cover on clip, tighten with a rubber band, the exhibition of stretching tight, little ventilation hole is pricked according to the specification of 0.5 cm x 0.5 centimetre above with sewing-needle, the fritter that cabbage leaves is shredded into 0.5 × 0.5 centimetre is evenly sprinkling upon on film, concentrate on film to attract diamond-back moth adult and evenly lay eggs, get another block plastics membrane cover on blade, prick with rubber band, prevent leaves water loss from wilting and being subjected to displacement, open bottleneck, by the diamond-back moth adult 1-3 of pairing to putting into, to screw on bottle cap.
After 24 hours, throw off film on dish leaf, dish leaf, the Eggs of Diamondback Moth produced on film can be clear that, on film, enclose ovum with carbon pen to draw a circle one by one, enclose a number one, until ovum on film has all been enclosed, if any more than 2 ovum closely, just circle is in a circle, ovum grain number in the mark circle of side, single ovum number and many ovum number with to be ovum initially total.
Smear lower rubber band, by marked egg membrane take off, film former state covered on the latter half mineral water bottle clip, tighten with rubber band the exhibition of stretching tight, notice that ovum is towards interior, index face is outside.
When have ovum color slightly blackening time, represent ovum be about to hatching, need observe every day.When looking into ovum, have a look at facing to bright place, existence or the disappearance of the interior ovum that goes too far can be seen.
Hatching early stage, hatching negligible amounts, first counts the scarce oval number of turns of single ovum, and fork is made in number limit, limit in circle, and enclose interior more than 2, from coil to coil checks quantity, and if any minimizing, number is hatched in the calculation of mark residue ovum gauge, two hatch quantity hatched total quantity the same day with being.Next time is when observing again, if hatching amount is few, still calculates as stated above and hatches quantity the same day.
Behind hatching peak, do not hatch ovum and tail off, directly can count residue ovum number, compare with proxima luce (prox. luc), calculate and hatched number the same day.When number is not hatched, draw √ aside, convenient next time finds counting simultaneously.
Claims (2)
1. a diamond-back moth gets ovum and egg hatching observation device, it is characterized in that: comprise and get egg apparatus and see egg apparatus, described get that egg apparatus comprises tubbiness get egg apparatus main body (1), getting egg apparatus the latter half is funnel-form, bottom is provided with seal cover, get egg apparatus main body (1) the first half and be provided with air-vent I(4), get egg apparatus main body (1) upper edge bundle and have an aperture (6), aperture (6) establishes the sliver (5) being inserted with and dipping in glucose solution, the bung place of getting egg apparatus main body (1) is provided with double-layer plastic film (2), double-layer plastic film (2) is tightened by rubber band and is being got in egg apparatus main body, double-layer plastic film (2) is provided with some air-vent II(7), double-layer plastic film (2) interlayer place is placed with cabbage leaves (3), described sight egg apparatus (8) is the barreled structure that diameter is identical with getting egg apparatus main body (1), sees egg apparatus (8) bung place and is provided with double-layer plastic film (2) from getting egg apparatus (1) and taking off.
2. diamond-back moth according to claim 1 gets ovum and egg hatching observation device, it is characterized in that: described egg apparatus main body (1) the first half of getting is provided with the air-vent I(4 distributed by 10 millimeters × 10 millimeters), aperture (6) is apart from getting egg apparatus main body (1) top edge 3-4 centimetre, air-vent II(7 on double-layer plastic film (2)) by 0.5 cm x 0.5 centimetre distribution, the size of cabbage leaves (3) is 0.5 cm x 0.5 centimetre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420704824.2U CN204305886U (en) | 2014-11-22 | 2014-11-22 | Diamond-back moth gets ovum and egg hatching observation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420704824.2U CN204305886U (en) | 2014-11-22 | 2014-11-22 | Diamond-back moth gets ovum and egg hatching observation device |
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| CN204305886U true CN204305886U (en) | 2015-05-06 |
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| CN201420704824.2U Expired - Fee Related CN204305886U (en) | 2014-11-22 | 2014-11-22 | Diamond-back moth gets ovum and egg hatching observation device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379684A (en) * | 2015-11-16 | 2016-03-09 | 郑竹胜 | Adult plutellaxylostella feeding bottle |
| CN107211969A (en) * | 2017-07-17 | 2017-09-29 | 上海市绿化管理指导站 | A kind of device and a kind of observational technique of aculea absorption tiny insect for limiting aculea absorption tiny insect scope of activities |
-
2014
- 2014-11-22 CN CN201420704824.2U patent/CN204305886U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379684A (en) * | 2015-11-16 | 2016-03-09 | 郑竹胜 | Adult plutellaxylostella feeding bottle |
| CN107211969A (en) * | 2017-07-17 | 2017-09-29 | 上海市绿化管理指导站 | A kind of device and a kind of observational technique of aculea absorption tiny insect for limiting aculea absorption tiny insect scope of activities |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150506 Termination date: 20151122 |
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| EXPY | Termination of patent right or utility model |