CN103081835A - Method of embedding nuclei inside hyriopsis schlegeli for breeding oversized round-shaped pearls - Google Patents
Method of embedding nuclei inside hyriopsis schlegeli for breeding oversized round-shaped pearls Download PDFInfo
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- CN103081835A CN103081835A CN2013100445814A CN201310044581A CN103081835A CN 103081835 A CN103081835 A CN 103081835A CN 2013100445814 A CN2013100445814 A CN 2013100445814A CN 201310044581 A CN201310044581 A CN 201310044581A CN 103081835 A CN103081835 A CN 103081835A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a method of embedding nuclei inside hyriopsis schlegeli for breeding oversized round-shaped pearls. Nucleus inserting is used once or twice. The method is characterized in that the nucleus inserting method includes the following steps: after a pearl breeding oyster opens and is added with a plug, and dirt inside the oyster is cleared away, using a wound needle to wound the position of half of the axe foot width and 40%-45% (counting from the back end, and the same below) of the axe foot length of the pearl breeding oyster to obliquely downwards pass a visceral mass towards a front end to wound horizontally, and then pulling out the needle in the position of 55%-65% of the visceral mass, and implanting a bead nucleus and cell bodies in the position of a wound, wherein the position of the implanted bead nucleus includes that a central point is in the position of 60%-75 % of the length of the visceral mass, the edge of the upper end of the bead nucleus adjoins the connecting position of an axe foot and the visceral mass, a nucleus sending needle is pulled out, the position of the wound is smoothed, and the plug is pulled out after inflammation is diminished in the wounds; after being operated, hanging and breeding the pearl breeding oyster in a repairing pool, after the wound heals, hanging and breeding the pearl breeding oyster in a breeding pool. Due to the facts that space in the visceral mass and the visceral mass positions of the oyster is large, an oversized pearl can be borne inside the oyster and cannot be squeezed. Therefore, oversized top-grade high-quality pearl products can be bred.
Description
Technical field
The present invention relates to a kind of core of planting and cultivate the circular pearl method of super large-scale in the Chidie clam visceral mass, belong to agriculture aquatic products technical field.
Background technology
existing in Chidie clam cultivation fresh water pearl production technology, from cultivation waters selection, freshwater mussel is cultivated in the operation preparation, pearl culturing clam operation inoculation, the pearl culturing clam aquaculture management, the sick prevention of cultivation waters water quality adjustment and freshwater mussel etc. has formed a ripe prior art of cover, the advantage of this technology is: cultivate high-grade high quality pearl in the outer embrane of Chidie clam, existing problems and deficiency are: due to the limitation of outer embrane thickness, be difficult to cultivate the positive circular nucleated pearl of super large-scale in outer embrane, also can tell because outer embrane can not bear in core and freshwater mussel the little and crimp in space even if implant large specification pearl core, therefore, more than namely enabling to reach 15mm, but can not reach orbicular high-grade gold standard.
Summary of the invention
The object of the invention is to by plant core in the Chidie clam visceral mass, Chidie clam outer embrane thickness and the limitation of planting space, core position have been solved, because the space of in visceral mass and visceral mass position freshwater mussel is large, can bear super large-scale pearl core, and can not be squeezed, therefore can cultivate the high-grade high quality pearl product of super large-scale, thereby provide a kind of core of planting in the Chidie clam visceral mass to cultivate the circular pearl method of super large-scale.
The present invention is achieved in that
A kind of core of planting in the Chidie clam visceral mass is cultivated the circular pearl method of super large-scale, adopts once or secondary-plug core, and nuclear insertion method is as follows:
After the pearl culturing clam opening is jumped a queue, wipe foul in freshwater mussel with medical silk floss, use the wound pin, start at from the rear end of pearl culturing clam, at long by 40-45%, wide half the place's wound of the abdominal foot of pearl culturing clam, the oblique lower visceral mass parallel forward end wound again that leads to is to internal organ regimental commander 55-65% place, extract the wound pin out, with sending the core pin to implant pearl core and mantle piece from wound; The position of implanting pearl core is: pearl core central point is at visceral mass length 65-75% place, pearl core upper end-face edge is near abdominal foot and visceral mass junction, pin pearl after sending into and examine and allocate out and send the core pin, then smooth the wound place, then dial unstopper after anti-inflammatory and namely complete operation inoculation operation; To inoculate good pearl culturing clam hanging culture in 2 hours after the slotting core of operation and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam.
Be that further method is as follows:
(1) nuclear insertion method for the first time:
operation method: after the pearl culturing clam opening is jumped a queue, the left side of freshwater mussel forward, ventral margin up, be placed on operation bracket, wipe foul in freshwater mussel with medical silk floss, use the wound pin, at abdominal foot long 40-45%, wide half place's wound, the oblique lower visceral mass parallel forward end wound again that leads to, to internal organ regimental commander 55-65% place, extract the wound pin out, put on a globule core with the taper end of sending the core pin from the aperture in the middle of core, put on a slice mantle piece from the middle of 3 * 3 millimeters square mantle pieces again, require the exocuticle of mantle piece to be close to globule core and connective tissue forward, afterwards, implant globule core from wound, the position of implanting globule core is: pearl core central point is at visceral mass length 65-75% place, pearl core upper end-face edge is near abdominal foot and visceral mass junction, send into and pin globule core with the elbow pin afterwards, the core pin is sent in dial-out, smooth the wound place with the wound pin again, after dripping anti-inflammatory medicine, group unstopper is namely completed the operation of operation inoculation for the first time,
To inoculate good pearl culturing clam hanging culture in 2 hours after the slotting core of operation and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam;
Being made as of mantle piece: with strip masking process, exocuticle in the pallial marginal membrane of film-making freshwater mussel is separated, be cut into 3 * 3 millimeters square mantle pieces [Xu Maoxi etc., Chidie clam grow cultured pearls production technology, " science is breeded fish " 2009.8.32] after exocuticle is repaired on one side get.
(2) nuclear insertion method for the second time:
operation method: after the pearl culturing clam opening is jumped a queue, the left side of freshwater mussel forward, ventral margin up, be placed on operation bracket, wipe foul in freshwater mussel with medical silk floss, find accurately for the first time at visceral mass and insert the pearl position that produces after core with getting the pearl device, use the wound pin, it is visceral mass edge wound below former wound place, along the pearl position forward logical wound to pearl edge, with getting the disposable taking-up pearl of pearl device, again with sending the core needle tip to put on a large pearl core from large pearl core interstitial hole, implant large pearl core from wound along wound to former pearl position, send into and pin large pearl core with the elbow pin afterwards, the core pin is sent in dial-out, smooth the wound place with the wound pin again, after dripping anti-inflammatory medicine, group unstopper is namely completed the operation of operation inoculation for the second time,
To inoculate good pearl culturing clam hanging culture in 2 hours after the slotting core of operation and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam;
When the pearl that produces after core that inserts for the first time of taking out has larger tail, dirty head, nacre not to wrap when complete, no longer implant large pearl core, be about to pearl culturing clam and eliminate.
Preferably: insert the core time: operation is planted season core time 5,6,7,9,10 or November.
When inserting core for the first time, the pearl culturing clam specification is long 13 ± 1 centimetres of freshwater mussel body.
First inserts core cultivation 14-18 months.
Globule core when inserting for the first time core is: making diameter with shell is the circular beads core of 8.5-9 millimeters, middle perforation of beating 0.8 ± 0.1 millimeter of diameter, inserting sterilizes before core dry standby.
Large pearl core when inserting for the second time core is: making diameter with shell is the circular beads core of 13.5-14 millimeters, middle half hole of beating 1.5 ± 0.1 millimeters of diameters, inserting sterilizes before core dry standby.
Second inserts core cultivation 14-18 months.
The effect of invention:
Plant core in the Chidie clam visceral mass, Chidie clam outer embrane thickness and the limitation of planting space, core position have been solved, the shell-to-shell distance that the outer embrane thickness of 13 centimetres of Chidie clams only has 1.5-2 millimeters, insert the core position only has 16 millimeters, and because the stretching motion of abdominal foot can rub to pearl, implant large specification pearl core can be because outer embrane can not bear, abdominal foot rubs broken outer embrane pearl is come off and freshwater mussel in the space is little that pearl is out of shape.It is 20 millimeters that the visceral mass of 13 centimetres of Chidie clams is planted core position thickness, and shell-to-shell distance has 35 millimeters, can bear super large-scale pearl core, can't be squeezed, and can cultivate the high-grade high quality pearl product of super large-scale.
First inserted the core cultivation after 14-18 months, the pearl culturing clam survival rate is more than 95%, firm-core rate is more than 98%, the nucleated pearl diameter of cultivating is at 12.5-13.5 millimeters, wherein: positive circular, pearl pearl layer light accounts for 50%(and wherein accounts for 20% without the high-grade high quality pearl of any flaw in fine and smooth, the surface of pearl layer light), tail is arranged but the pearl of pearl layer light accounts for 40%, a dirt pearl accounts for 5%, nacre does not wrap completely that pearl accounts for 5%.
Second inserted the core cultivation after 14-18 months, the pearl culturing clam survival rate is more than 90%, firm-core rate is more than 95%, the nucleated pearl diameter of cultivating is in 19 millimeter, wherein: positive circular, pearl pearl layer light accounts for 70%(and wherein accounts for 50% without the high-grade high quality pearl of any flaw in fine and smooth, the surface of pearl layer light), tail is arranged but the pearl of pearl layer light accounts for 25%, a dirt pearl accounts for 5%.
Description of drawings
Fig. 1 is that the embodiment of the present invention 1 first is inserted the wound position of core and plants the nuclear location schematic diagram.
Fig. 2 is that the embodiment of the present invention 1 second is inserted the wound position of core and plants the nuclear location schematic diagram.
In figure: the first wound position 1-1, the first of inserting core is inserted core and is planted nuclear location 1-2, the second and insert the wound position 2-1, the second of core and insert core and plant nuclear location 2-2.
Embodiment
The present invention can specifically implement by the technology contents that illustrates in summary of the invention, can conduct further description the present invention by the following examples, yet scope of the present invention is not limited to following embodiment.
Embodiment 1:
(1) plant the core time: operation plant season core time 5,6,7,9,10, November.
(2) insert for the first time core: the pearl culturing clam specification requirement; The long 13 centimetres of left and right of freshwater mussel body;
Operation tool: opener, 2, stainless steel stopper, wound cutter are (with the stainless steel bar of 3 millimeters of diameters, 18 millimeters of length, flatten the sword shape blade that forms top point, middle 6 mm wides for one, and wear into sharp wound cutter), the elbow pin is (with 2 millimeters of diameters, the stainless steel bar of long 20 millimeters, a levigate and hook), send core pin (with the stainless steel bar of 2.5 millimeters of diameters, 25 millimeters of length, a circular fine needle of wearing into 0.8 millimeter of diameter, 13 millimeters of length).
Globule core: making diameter with shell is the circular globule core of 8.5-9 millimeters, middle perforation of beating 0.8 millimeter of diameter, inserting sterilizes before core dry standby.
operation method: after the pearl culturing clam opening is jumped a queue, the left side of freshwater mussel forward, ventral margin up, be placed on operation bracket, wipe gently foul in freshwater mussel with medical silk floss, the right hand is taken the wound pin, grow 40-45%(from the rear end at abdominal foot), wide half place's wound, the oblique lower visceral mass parallel forward end wound again that leads to, to internal organ regimental commander 60% place (from the rear end), extract the wound pin out, put on a globule core with the taper end of sending the core pin from the aperture in the middle of core, (exocuticle of mantle piece is close to globule core to put on a slice mantle piece from the middle of 3 * 3 millimeters square mantle pieces again, connective tissue is forward) after, implant globule core from wound, the position of implanting globule core is: globule core central point is at visceral mass length 70% place (from the rear end), globule core upper end-face edge is near abdominal foot and visceral mass junction, send into and pin globule core with the elbow pin afterwards, the core pin is sent in dial-out, smooth the wound place with the wound pin again, after dripping anti-inflammatory medicine, group unstopper is namely completed the operation of operation inoculation for the first time.
Perform the operation and will inoculate good pearl culturing clam hanging culture in rear 2 hours and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam.
Insert for each person every day pearl culturing clam 900 left and right.
First inserted the core cultivation after 14-18 months, the pearl culturing clam survival rate is more than 95%, firm-core rate is more than 98%, the nucleated pearl diameter of cultivating is at 12.5-13.5 millimeters, wherein: positive circular, pearl pearl layer light accounts for 50%(and wherein accounts for 20% without the high-grade high quality pearl of any flaw in fine and smooth, the surface of pearl layer light), tail is arranged but the pearl of pearl layer light accounts for 40%, a dirt pearl accounts for 5%, nacre does not wrap completely that pearl accounts for 5%.
(2) insert for the second time core:
Operation tool: opener, 2, stainless steel stopper, wound cutter, the same operation tool for the first time of elbow pin, get the pearl device (with the stainless steel bar of 4 millimeters of diameters, 25 millimeters of length, 2 millimeters thin rods of diameter are worn at two ends, and bend to positive circle, the diameter of two ends circle is respectively 10 millimeters and 11.5 millimeters), send core pin (with the stainless steel bar of 4 millimeters of diameters, 18 millimeters of length, an end is worn into the pointed of pencil head).
Large pearl core: making diameter with shell is the circular large pearl core of 13.5-14 millimeters, middle half hole of beating 1.5 millimeters of diameters, inserting sterilizes before core dry standby.
operation method: after the pearl culturing clam opening is jumped a queue, the left side of freshwater mussel forward, ventral margin up, be placed on operation bracket, wipe gently foul in freshwater mussel with medical silk floss, find the position of inserting for the first time the pearl that produces after core accurately at visceral mass with getting the pearl device, the right hand is taken the wound pin, below former wound place, visceral mass edge wound, along the pearl position forward logical wound to pearl edge, enter from wound with getting the pearl device, be deep into the below of pearl along wound, entangle pearl with circle, disposable taking-up pearl, again with sending the core needle tip to put on a large pearl core from large pearl core interstitial hole, implant large pearl core from wound along wound to former pearl position, send into and pin large pearl core with the elbow pin afterwards, the core pin is sent in dial-out, smooth the wound place with the wound pin again, after dripping anti-inflammatory medicine, group unstopper is namely completed the operation of operation inoculation for the first time.
Perform the operation and will inoculate good pearl culturing clam hanging culture in rear 2 hours and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam.
Insert for each person every day pearl culturing clam 600 left and right.
When the pearl that produces after core that inserts for the first time of taking out has larger tail, dirty head, when nacre does not wrap and waits pearl fully, no longer implants large pearl core, pearl culturing clam is eliminated.
Second inserted the core cultivation after 14-18 months, the pearl culturing clam survival rate is more than 90%, firm-core rate is more than 95%, the nucleated pearl diameter of cultivating is in 19 millimeter, wherein: positive circular, pearl pearl layer light accounts for 70%(and wherein accounts for 50% without the high-grade high quality pearl of any flaw in fine and smooth, the surface of pearl layer light), tail is arranged but the pearl of pearl layer light accounts for 25%, a dirt pearl accounts for 5%.
Claims (8)
1. plant the circular pearl method of core cultivation super large-scale for one kind in the Chidie clam visceral mass, adopt once or secondary-plug core, it is characterized in that nuclear insertion method is as follows:
After the pearl culturing clam opening is jumped a queue, wipe foul in freshwater mussel with medical silk floss, use the wound pin, start at from the rear end of pearl culturing clam, at long by 40-45%, wide half the place's wound of the abdominal foot of pearl culturing clam, the oblique lower visceral mass parallel forward end wound again that leads to is to internal organ regimental commander 55-65% place, extract the wound pin out, with sending the core pin to implant pearl core and mantle piece from wound; The position of implanting pearl core is: pearl core central point is at visceral mass length 65-75% place, pearl core upper end-face edge is near abdominal foot and visceral mass junction, pin pearl after sending into and examine and allocate out and send the core pin, then smooth the wound place, then dial unstopper after anti-inflammatory and namely complete operation inoculation operation; To inoculate good pearl culturing clam hanging culture after slotting core in 2 hours and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam.
2. a kind of core of planting in the Chidie clam visceral mass according to claim 1 is cultivated the circular pearl method of super large-scale, it is characterized in that method is as follows:
(1) nuclear insertion method for the first time:
after the pearl culturing clam opening is jumped a queue, the left side of freshwater mussel forward, ventral margin up, be placed on operation bracket, wipe foul in freshwater mussel with medical silk floss, use the wound pin, at abdominal foot long 40-45%, wide half place's wound, the oblique lower visceral mass parallel forward end wound again that leads to, to internal organ regimental commander 55-65% place, extract the wound pin out, put on a globule core with the taper end of sending the core pin from the aperture in the middle of core, put on a slice mantle piece from the middle of 3 * 3 millimeters square mantle pieces again, require the exocuticle of mantle piece to be close to globule core and connective tissue forward, afterwards, implant globule core from wound, the position of implanting globule core is: pearl core central point is at visceral mass length 65-75% place, pearl core upper end-face edge is near abdominal foot and visceral mass junction, send into and pin globule core with the elbow pin afterwards, the core pin is sent in dial-out, smooth the wound place with the wound pin again, after dripping anti-inflammatory medicine, group unstopper is namely completed the operation of operation inoculation for the first time,
To inoculate good pearl culturing clam hanging culture after slotting core in 2 hours and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam;
(2) nuclear insertion method for the second time:
after the pearl culturing clam opening is jumped a queue, the left side of freshwater mussel forward, ventral margin up, be placed on operation bracket, wipe foul in freshwater mussel with medical silk floss, find accurately for the first time at visceral mass and insert the pearl position that produces after core with getting the pearl device, use the wound pin, it is visceral mass edge wound below former wound place, along the pearl position forward logical wound to pearl edge, with getting the disposable taking-up pearl of pearl device, again with sending the core needle tip to put on a large pearl core from large pearl core interstitial hole, implant large pearl core from wound along wound to former pearl position, send into and pin large pearl core with the elbow pin afterwards, the core pin is sent in dial-out, smooth the wound place with the wound pin again, after dripping anti-inflammatory medicine, group unstopper is namely completed the operation of operation inoculation for the second time,
To inoculate good pearl culturing clam hanging culture after slotting core in 2 hours and repair in the pond to the pearl culturing clam postoperative, after wound healing, hanging culture is cultivated in the pond to pearl culturing clam;
When the pearl that produces after core that inserts for the first time of taking out has larger tail, dirty head, nacre not to wrap when complete, no longer implant large pearl core, be about to pearl culturing clam and eliminate.
3. a kind of core of planting in the Chidie clam visceral mass according to claim 1 and 2 is cultivated the circular pearl method of super large-scale, it is characterized in that inserting the core time: operation is planted season core time 5,6,7,9,10 or November.
4. a kind of core of planting in the Chidie clam visceral mass according to claim 2 is cultivated the circular pearl method of super large-scale, and when it is characterized in that inserting core for the first time, the pearl culturing clam specification is long 13 ± 1 centimetres of freshwater mussel body.
5. a kind of core of planting in the Chidie clam visceral mass according to claim 2 is cultivated the circular pearl method of super large-scale, it is characterized in that first inserts core cultivation 14-18 months.
6. a kind of core of planting in the Chidie clam visceral mass according to claim 2 is cultivated the circular pearl method of super large-scale, globule core when it is characterized in that inserting core for the first time is: making diameter with shell is the circular beads core of 8.5-9 millimeters, middle perforation of beating 0.8 ± 0.1 millimeter of diameter is inserted the front sterilization of core and is dried standby.
7. a kind of core of planting in the Chidie clam visceral mass according to claim 2 is cultivated the circular pearl method of super large-scale, large pearl core when it is characterized in that inserting core for the second time is: making diameter with shell is the circular beads core of 13.5-14 millimeters, middle half hole of beating 1.5 ± 0.1 millimeters of diameters is inserted the front sterilization of core and is dried standby.
8. a kind of core of planting in the Chidie clam visceral mass according to claim 2 is cultivated the circular pearl method of super large-scale, it is characterized in that second inserts core cultivation 14-18 months.
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| CN103355229A (en) * | 2013-06-06 | 2013-10-23 | 金华职业技术学院 | Immunostimulation generation method of pearl sac of pearl mussel |
| CN103621445A (en) * | 2013-12-02 | 2014-03-12 | 浙江海洋学院 | Method for inserting nucleus in pear mussel |
| CN103947583A (en) * | 2014-04-02 | 2014-07-30 | 江西万年县凤珠实业有限公司 | Cultivation method for large-particle high-quality freshwater pearls |
| CN104969890A (en) * | 2015-07-09 | 2015-10-14 | 谢绍河 | Cultiving method for implanting pearl core and nursing pearls via giant clam shells |
| CN105309341A (en) * | 2014-07-23 | 2016-02-10 | 郑波明 | Nucleus insertion method in seawater pearl culture nucleus insertion and pearl culture operation |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355229A (en) * | 2013-06-06 | 2013-10-23 | 金华职业技术学院 | Immunostimulation generation method of pearl sac of pearl mussel |
| CN103355229B (en) * | 2013-06-06 | 2015-01-07 | 金华职业技术学院 | Immunostimulation generation method of pearl sac of pearl mussel |
| CN103621445A (en) * | 2013-12-02 | 2014-03-12 | 浙江海洋学院 | Method for inserting nucleus in pear mussel |
| CN103621445B (en) * | 2013-12-02 | 2015-10-07 | 浙江海洋学院 | Nacreous nuclear insertion method |
| CN103947583A (en) * | 2014-04-02 | 2014-07-30 | 江西万年县凤珠实业有限公司 | Cultivation method for large-particle high-quality freshwater pearls |
| CN105309341A (en) * | 2014-07-23 | 2016-02-10 | 郑波明 | Nucleus insertion method in seawater pearl culture nucleus insertion and pearl culture operation |
| CN104969890A (en) * | 2015-07-09 | 2015-10-14 | 谢绍河 | Cultiving method for implanting pearl core and nursing pearls via giant clam shells |
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