CN102618486B - Culture medium for culturing cell slices of blue pearls - Google Patents
Culture medium for culturing cell slices of blue pearls Download PDFInfo
- Publication number
- CN102618486B CN102618486B CN2011104615779A CN201110461577A CN102618486B CN 102618486 B CN102618486 B CN 102618486B CN 2011104615779 A CN2011104615779 A CN 2011104615779A CN 201110461577 A CN201110461577 A CN 201110461577A CN 102618486 B CN102618486 B CN 102618486B
- Authority
- CN
- China
- Prior art keywords
- pearl
- culture medium
- blue
- cell slices
- mantle piece
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium for culturing cell slices of blue pearls. The culture medium comprises the following raw materials: in terms of 1000 ml of deionized water, 10 to 20 g of copper hydroxide, 9 g of sodium chloride, 10 to 20 g of cytochrome C, 5 to 10 g of glucose and 1 to 2 g of phosphatidylcholine. The culture medium for culturing cell slices of blue pearls can successfully culture blue pearls on the basis of improving the survival rate of cell slices.
Description
Technical field
The present invention relates to a kind of nutrient solution, belong to pearl pearl core processing technique field.
background technology
The cultivation of fresh water pearl is generally without nucleus pearl, and the method for employing is to insert mantle piece in the mantle position of freshwater mussel, and the pearl that the method is produced is called without nucleus pearl.The color and luster of pearl surface is generally white, faint yellow the colored pearls such as a small amount of gold, purple is also arranged, and the natural formation of these pearls mainly relies on the metal ion content of aquaculture water and sunlight etc. to act on absorption in mantle piece secretion pearl matter process and produces color.Also there is at present the trial of propagating the production colored pearl artificially not high because of reason success ratios such as cell rejection, pathologies eventually.Tracing it to its cause is mainly that the nutrient solution quality used does not pass a test and causes, and lacks the comprehensive means such as sterilization, sterilization, maintenance, healing.
Chinese invention patent number of patent application 02148618.2 discloses a kind of method for pearl culture, especially color pearl growing method.It is to be ready to the band such as rose the colorful one animal and plant nutriment, before Margarita being inserted to the core operation, pearl nucleus, transplanted cells sheet are carried out to the colour biological treatment, pass through again the cell sheet transplanting and plant pearl, the enchylema secretion produced in Margarita being planted after pearl cultivated is transmitted, the shellfish of performing the operation completes cultivation in seawater, is bred as colored pearl.Although this kind of cultural method cultivated colored pearl, do not mention nutrient solution formula function problem.
summary of the invention
The invention provides the mantle piece nutrient solution that a kind of pearl matter that improves surviving rate, impels mantle piece to secrete is the blue pearl of cultivation of blue gloss shape.
A kind of mantle piece nutrient solution of cultivating blue pearl is comprised of following several raw materials: in the 1000ml deionized water, wherein add:
Copper hydroxide: 10-20g,
Sodium-chlor: 9g,
Cytochrome C: 10-20g,
Glucose 5-10g, Yelkin TTS 1-2g.
Mantle piece is the core element that plays secretion pearl matter in the seedless pearl culture of fresh water, mantle piece obtain general employing shellfish jacket membrane epithelium as mantle piece, the steps include:
1. in shellfish jacket membrane epithelium colo(u)r streak, smooth, firmly evenly resolute, the degree of depth reaches the mantle middle junction and forms the standardized cutter of organized layer and get final product.
2. dyestripping should be from mantle be anterior, tears in step 1 standardized cutter place and obtains and organize small pieces.
3. tweezers folder absorbent cotton arranged, arrange in step 2 and organize small pieces, wipe mucus etc.
4. after organizing small pieces to be placed on sheet glass, should be by both sides uneven surface equating, width uniformity.The small pieces that are cut into require 4 millimeters * 5 millimeters, after shrinking, are square.The section requirement is resolute, clear-cut, otch smoothly obtains mantle piece.
5. should drip immediately the mantle piece of cultivating blue pearl after the section and require the uniform fold mantle piece with nutrient solution.
6. sheet glass thimerosal sterilization, mantle piece can not contact other things, strictly prevent from polluting.
7. film-making process is completed continuously by a people, requires to finish in 2 minutes, and, in time for inoculation, can not deposit too of a specified duration.
The mantle piece surface attachment of this kind of process acquisition has copper hydroxide, sodium-chlor, cytochrome C, glucose, five kinds of benefit materials of Yelkin TTS.Copper hydroxide is blue flocks, and the cupric ion of copper hydroxide has germicidal action, the secretion of irritation cell small pieces, thereby because copper hydroxide is that its pearl matter by the secretion of fused cell small pieces of blue flocks makes pearl matter obtain blue pearl for blueness.Sodium chloride solution is that a kind of sterilization, anti-inflammation drugs cheap, that effect is good prevent the mantle piece wound infection.Cytochrome C is the electron transit mediator in biological oxidation process, and when anoxic, the permeability of cytolemma increases, and cytochrome C just can enter in cell and plastosome, strengthens cellular oxidation, the utilization that can improve oxygen, the effect of correcting cellular respiration and substance metabolism.Glucose is the indispensable nutritive substance of organism intracellular metabolic.Its oxidizing reaction liberated heat is the important sources of mantle piece vital movement institute energy requirement.Yelkin TTS can promote the hyperplasia of Main Tissues composition in reticular tissue, accelerates coincideing between mantle piece reticular tissue and operation freshwater mussel tissue, shortens the osmotrophy phase of mantle piece in allosome tissue, and the formation of accelerating pearl sac improves surviving rate greatly.
The mantle piece of the blue pearl of cultivation provided by the invention is successfully cultivated blue pearl with nutrient solution on the basis of improving the mantle piece surviving rate.
Embodiment
The peace following steps are obtained the mantle piece of cultivating blue pearl
1. in shellfish jacket membrane epithelium colo(u)r streak, smooth, firmly evenly resolute, the degree of depth reaches the mantle middle junction and forms the standardized cutter of organized layer and get final product.
2. dyestripping should be from mantle be anterior, tears in step 1 standardized cutter place and obtains and organize small pieces.
3. tweezers folder absorbent cotton arranged, arrange in step 2 and organize small pieces, wipe mucus etc.
4. after organizing small pieces to be placed on sheet glass, by both sides uneven surface equating, width uniformity.The small pieces that are cut into require 4 millimeters * 5 millimeters, after shrinking, are square.The section requirement is resolute, clear-cut, otch smoothly obtains mantle piece.
5. should drip immediately the mantle piece of cultivating blue pearl after the section and require the uniform fold mantle piece with nutrient solution.
6. sheet glass thimerosal sterilization, mantle piece can not contact other things, strictly prevent from polluting.
7. film-making process is completed continuously by a people, requires to finish in 2 minutes, and, in time for inoculation, can not deposit too of a specified duration.
The mantle piece of cultivating blue pearl in above-mentioned steps by the nutrient solution proportioning is:
In the 1000ml deionized water, wherein add
Claims (1)
1. a mantle piece nutrient solution of cultivating blue pearl is comprised of following several raw materials:
In the 1000ml deionized water, wherein add:
Copper hydroxide: 10-20g,
Sodium-chlor: 3-5g,
Cytochrome C: 10-20g,
Glucose 5-10g,
Yelkin TTS 1-2g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104615779A CN102618486B (en) | 2011-12-31 | 2011-12-31 | Culture medium for culturing cell slices of blue pearls |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104615779A CN102618486B (en) | 2011-12-31 | 2011-12-31 | Culture medium for culturing cell slices of blue pearls |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102618486A CN102618486A (en) | 2012-08-01 |
| CN102618486B true CN102618486B (en) | 2013-12-25 |
Family
ID=46558699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104615779A Expired - Fee Related CN102618486B (en) | 2011-12-31 | 2011-12-31 | Culture medium for culturing cell slices of blue pearls |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102618486B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210855B (en) * | 2013-03-27 | 2014-11-05 | 中国科学院南海海洋研究所 | Mantle piece treatment liquid for improving seawater pearl quality and treatment method |
| CN103404460B (en) * | 2013-08-13 | 2014-12-17 | 北海市珍源海洋生物有限公司 | Cultivation raw material and method for black rose pearl growing factors |
| CN112586413A (en) * | 2020-12-31 | 2021-04-02 | 广东尊鼎珍珠有限公司 | Golden yellow seawater pearl cultivation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724598A (en) * | 2009-11-24 | 2010-06-09 | 广东海洋大学 | Novel maintenance liquid for mantle pieces of pinctada martensii and preparation method thereof |
| CN101804073A (en) * | 2009-07-17 | 2010-08-18 | 广东海洋大学 | Preparation method of pearl producing immune reinforcing agent |
-
2011
- 2011-12-31 CN CN2011104615779A patent/CN102618486B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101804073A (en) * | 2009-07-17 | 2010-08-18 | 广东海洋大学 | Preparation method of pearl producing immune reinforcing agent |
| CN101724598A (en) * | 2009-11-24 | 2010-06-09 | 广东海洋大学 | Novel maintenance liquid for mantle pieces of pinctada martensii and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王梅芳等.育珠贝细胞小片及处理技术研究进展.《广东海洋大学学报》.2010,第30卷(第4期),第86－90页. |
| 育珠贝细胞小片及处理技术研究进展;王梅芳等;《广东海洋大学学报》;20100831;第30卷(第4期);第86－90页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102618486A (en) | 2012-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101491228B (en) | Sea no-nucleus pearl incubation method | |
| CN101491229B (en) | Vitro culturing method of pearl shell mantle tissue and culture medium | |
| CN102037856B (en) | Simple cordyceps militaris strain rejuvenation method | |
| CN101803581A (en) | Method for cultivating large granular nuclear pearls | |
| CN103563746A (en) | Method for culturing shoot apical meristem of dendranthema morifolium pamat | |
| CN103636533A (en) | Method for cultivating freshwater nucleated pearls | |
| CN103478028A (en) | Directional cultivation method of white or purple fresh water pearl | |
| CN102618486B (en) | Culture medium for culturing cell slices of blue pearls | |
| WO2015033973A1 (en) | Mother-of-pearl, mother-of-pearl production method, and pearl production method | |
| CN101642051A (en) | Rapid propagation technology with open tissue culture for xanthoceras sorbifolia bunge | |
| CN103931494B (en) | A kind of method of color cotton ovules culture in vitro | |
| CN102845218A (en) | Original ecological separation method of cordyceps sinensis hirsutella sinensis | |
| CN104756915B (en) | A kind of breeding method of colorful sea water pearls | |
| CN105132354B (en) | A kind of extracting method of eddo fragrant plant mentioned in ancient texts protoplast | |
| CN103598129A (en) | Method for cultivating iridescence fresh water pearls | |
| CN102283153A (en) | Batch production fry rearing technique for exopalaemon modestus | |
| CN103314911B (en) | A kind of breeding method of natural seawater pearl | |
| CN103340168B (en) | Special fresh water pearl cultivating method based on small seawater shellfish cell pieces | |
| CN109717080A (en) | A method of improving Afriocan agapanthus cells,primordial subculture effect | |
| CN108496862A (en) | Golden pearl cultivation method | |
| CN109588377B (en) | Silkworm egg hatching method | |
| CN103141425A (en) | Producing method of sterile zebra fish | |
| CN112586413A (en) | Golden yellow seawater pearl cultivation method | |
| CN103210855B (en) | Mantle piece treatment liquid for improving seawater pearl quality and treatment method | |
| CN104982363A (en) | Culture solution for cultivating cell pieces of blood red pearls |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131225 Termination date: 20141231 |
|
| EXPY | Termination of patent right or utility model |