CN103340168B - Special fresh water pearl cultivating method based on small seawater shellfish cell pieces - Google Patents
Special fresh water pearl cultivating method based on small seawater shellfish cell pieces Download PDFInfo
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- CN103340168B CN103340168B CN201310280086.3A CN201310280086A CN103340168B CN 103340168 B CN103340168 B CN 103340168B CN 201310280086 A CN201310280086 A CN 201310280086A CN 103340168 B CN103340168 B CN 103340168B
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- shellfish
- pearl
- mussel
- seawater
- shell
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- 235000015170 shellfish Nutrition 0.000 title claims abstract description 51
- 239000013535 sea water Substances 0.000 title claims abstract description 28
- 239000013505 freshwater Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000237536 Mytilus edulis Species 0.000 claims abstract description 26
- 235000020638 mussel Nutrition 0.000 claims abstract description 26
- 210000002808 connective tissue Anatomy 0.000 claims abstract description 6
- 241000272194 Ciconiiformes Species 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 238000012423 maintenance Methods 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 241000255969 Pieris brassicae Species 0.000 claims description 9
- 238000009360 aquaculture Methods 0.000 claims description 8
- CYDMQBQPVICBEU-UHFFFAOYSA-N chlorotetracycline Natural products C1=CC(Cl)=C2C(O)(C)C3CC4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-UHFFFAOYSA-N 0.000 claims description 6
- CYDMQBQPVICBEU-XRNKAMNCSA-N chlortetracycline Chemical compound C1=CC(Cl)=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-XRNKAMNCSA-N 0.000 claims description 6
- 229960004475 chlortetracycline Drugs 0.000 claims description 6
- 235000019365 chlortetracycline Nutrition 0.000 claims description 6
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- 238000012805 post-processing Methods 0.000 claims description 5
- 210000001519 tissue Anatomy 0.000 claims description 5
- 229910000906 Bronze Inorganic materials 0.000 claims description 3
- 241000872198 Serjania polyphylla Species 0.000 claims description 3
- 239000010974 bronze Substances 0.000 claims description 3
- KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 239000011049 pearl Substances 0.000 description 40
- 241000879903 Pteria Species 0.000 description 3
- 239000010984 cultured pearl Substances 0.000 description 3
- 241000382825 Cristaria plicata Species 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 240000007133 Cordia dichotoma Species 0.000 description 1
- 241001005836 Euchloe ausonia Species 0.000 description 1
- 241000452298 Sinanodonta woodiana Species 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000010437 gem Substances 0.000 description 1
- 229910001751 gemstone Inorganic materials 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a special fresh water pearl cultivating method based on small seawater shellfish cell pieces. The pallium of a seawater shellfish is cut into small pieces, the small pieces are inserted in connective tissue of the pallium of a fresh water mussel, and a special pearl can be cultivated in fresh water after 2-3 years through processing.
Description
Technical field
The invention belongs to pearl culture field, the characteristic pearl cultivating method that especially seawater shellfish and mussel combine.
Background technology
Pearl, as jewel queen, enjoys people's favor always, and it,, from source minute, has two classes: seawater pearl and fresh water pearl.Fresh water pearl and seawater pearl are generally speaking more easy to identify, because seawater pearl color and luster is better than fresh water pearl, and individual larger than fresh water pearl, shape circle.Seawater pearl is beautiful graceful, the sense that is translucent, and to a kind of soft dim U.S. of people, this is attributed to its gloss to a great extent, and the rounded probability of seawater pearl is higher than fresh water pearl, and this is also to be determined by its inner concentric circular layer structure.The measured superior seawater pearl of matter is gram to determine the price, and its value can be considerably beyond gold.Fresh water pearl is as the raw pearl of freshwater mussel, than seawater pearl, is being worth and a poor qualitatively class.Sea water pearls is mainly derived from the shellfish kinds such as white butterfly shell, black lip shell, penguin shellfish and pteria martensii at present, first three plants producing region mostly abroad, the main breed variety of China is pteria martensii, its pearl color be take white as main, by contrast, external black lip shell and penguin shellfish respectively the same grade of cost ratio of institute's black that produces and bronze-colored pearl white pearl costliness a lot.Because China's freshwater mussel body survival rate is high, and freshwater aquiculture field is wide, therefore seawater shellfish black lip shell, penguin shellfish is combined and cultivates the active demand that characteristic pearl has been complied with China cultured pearl field just with mussel respectively.Nowadays adopt shellfish jacket membrane patch-graft interior to cultivate the method for pearl to pearly shellfish body, it is the main technique of artificial culture pearl always, at present the method is grown cultured pearls in field and is all obtained some successes separately seawater shellfish and mussel, pan colour system pearl after the small pieces of producing as cristaria plicata are implanted in hydriopsis cumingii body; The small pieces that circle anodonta woodiana pacifica is produced produce pink pearl after implanting in cristaria plicata body; It is pearl that the small pieces that black lip shell is produced produce black after implanting in pteria martensii body; Yet this method is applied to seawater shellfish and mussel have not been reported in conjunction with field, therefore seawater shellfish black lip shell, penguin shellfish are combined and cultivate characteristic pearl with mussel respectively, and in freshwater aquiculture expanding production, be the new breakthrough in China cultured pearl field.
Summary of the invention
The object of the invention is to provide a kind of for characteristic seawater Zhu China's output problem on the low side seawater shellfish and mussel are combined to the method for cultivating characteristic pearl.The applicable seawater shellfish of the present invention is black lip shell, white butterfly shell and penguin shellfish, mussel is hydriopsis cumingii, and it is pearl that its characteristic is to cultivate the black with black lip shell pearl feature, have the white color system pearl of white butterfly shell pearl feature and have the bronze colour system pearl of penguin conch feature.
For achieving the above object, the present invention adopts following technical scheme:
(1), selection and the film-making of piece shell: selecting seawater shellfish is black lip shell, white butterfly shell or penguin shellfish, and shellfish body is intact, and the age, body was grown 4～6cm at 1～2 years old.From seawater shellfish, take off outer embrane purified treatment, require first to use aureomycin solution rinsing 5～8 times, aureomycin solution concentration is 500～1000U/mL, use again cobalt chloride solution rinsing 3～5 times, cobalt chloride solution concentration is 100～125 μ mol/L, after section, drip in time the tissue juice maintenance of former shellfish, require small pieces thickness to be: 0.5～0.8mm, size specification is: 1.5～3mm * 1.5～3mm, film-making process completes in 1～4min, and in time for inoculation, can not deposit too of a specified duration, generally in 0.5～2.5 hour inoculation for good.
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in mussel as pearly shellfish, require freshwater mussel body intact, the seedless mussel age, the long 4～6cm of body, had the core mussel age at 1.5～2.5 years old at 0.5～1 years old, the long 6～8cm of body.The made small pieces of step (1) are inserted in the pallial connective tissue of mussel.
(3) plant sheet post processing: the mussel of planting after sheet is first placed in clear water reserviors and is recuperated, change clear water every day to keep water cleaning, as changed clear water every day 1～3 time, carry out oxygen cycle simultaneously, maintenance oxygen concentration is 5～7mg/L, transfers to the cultivation of freshwater aquiculture district after 8～15 days again.
Advantage of the present invention: seawater shellfish and mussel are simple and feasible in conjunction with the method for cultivating characteristic pearl, have not only improved the value of existing pearl, and have been suitable for a large amount of production.
Embodiment
By specific embodiment, technical scheme of the present invention is made to concrete exemplary illustration below, be not construed as limiting the invention.
Embodiment 1:
(1), selection and the film-making of piece shell: select black lip shell, shellfish body is intact, 2 years old age, the long 5cm of body; From black lip shell, take off outer embrane and carry out purified treatment, first use the aureomycin solution rinsing 7 times of 800U/mL, then use the cobalt chloride solution rinsing 5 times of 125 μ mol/L; Outer embrane after purified treatment is cut and dripped into pieces to the tissue juice maintenance of former shellfish, small pieces thickness is 0.6mm, and size specification is: 3mm * 3mm, and whole film-making process completes with 2.5min;
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in mussel as pearly shellfish, seedless, intact, 1 years old age, the long 6cm of body, is inserted into the made small pieces of step (1) in the pallial connective tissue of hydriopsis cumingii in 1 hour after section completes;
(3), plant sheet post processing: the hydriopsis cumingii of planting after sheet is first placed in clear water reserviors and is recuperated, change clear water every day one time, carry out oxygen cycle simultaneously, maintenance oxygen concentration is 6mg/L, after 14 days, transfer to again the cultivation of freshwater aquiculture district, cultivate after 3 years, producing the black with black lip shell pearl feature is pearl.
Embodiment 2:
(1), selection and the film-making of piece shell: select white butterfly shell, shellfish body is intact, 1.5 years old age, the long 4cm of body; From white butterfly shell, take off outer embrane and carry out purified treatment, first use the aureomycin solution rinsing 6 times of 1000U/mL, then use the cobalt chloride solution rinsing 4 times of 125 μ mol/L; Outer embrane after purified treatment is cut and dripped into pieces to the tissue juice maintenance of former shellfish, small pieces thickness is 0.6mm, and size specification is: 3mm * 3mm, and whole film-making process completes with 2.5min;
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in mussel as pearly shellfish, seedless, intact, 0.5 years old age, the long 5cm of body, is inserted into the made small pieces of step (1) in the pallial connective tissue of hydriopsis cumingii in 0.5 hour after section completes;
(3), plant sheet post processing: the hydriopsis cumingii of planting after sheet is first placed in clear water reserviors and is recuperated, change clear water every day one time, carry out oxygen cycle simultaneously, maintenance oxygen concentration is 6mg/L, after 14 days, transfer to again the cultivation of freshwater aquiculture district, cultivate after 4 years, produce the white color system pearl with white butterfly shell pearl feature.
Embodiment 3:
(1), selection and the film-making of piece shell: select penguin shellfish, shellfish body is intact, 1.5 years old age, the long 5cm of body; From penguin shellfish, take off outer embrane and carry out purified treatment, first use the aureomycin solution rinsing 6 times of 1000U/mL, then use the cobalt chloride solution rinsing 5 times of 100 μ mol/L; Outer embrane after processing is cut and dripped into pieces to the tissue juice maintenance of former shellfish, small pieces thickness is 0.6mm, and size specification is: 3mm * 3mm, and whole film-making process completes with 3min;
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in mussel as pearly shellfish, there is core, intact, 2 years old age, the long 7cm of body, is inserted into the made small pieces of step (1) in the pallial connective tissue of hydriopsis cumingii in 0.5 hour after section completes;
(3), plant sheet post processing: the hydriopsis cumingii of planting after sheet is first placed in clear water reserviors and is recuperated, change clear water every day one time, carry out oxygen cycle simultaneously, maintenance oxygen concentration is 6mg/L, after 14 days, transfer to again the cultivation of freshwater aquiculture district, cultivate after 2 years, produce the bronze colour system pearl with penguin conch feature.
Claims (4)
1. the characteristic fresh water pearl breeding method based on seawater shellfish mantle piece, comprises the following steps:
(1) selection of piece shell and film-making: selecting shellfish body is seawater shellfish, intact, age was at 1～2 years old, long 4～the 6cm of body, from seawater shellfish, take off outer embrane and carry out purified treatment, the outer embrane after purified treatment is cut and dripped into pieces to the tissue juice maintenance of former shellfish, requiring small pieces thickness is 0.5～0.8mm, size specification is 1.5～3mm * 1.5～3mm, and film-making process completes in 1～4min;
(2) selection of pearly shellfish and plant sheet: select shellfish body to be mussel, intact, the seedless mussel age, the long 4～6cm of body, had the core mussel age at 1.5～2.5 years old at 0.5～1 years old, the long 6～8cm of body; The made small pieces of step (1) are inserted in the pallial connective tissue of mussel after film-making completes in 0.5～2.5 hour;
(3) plant sheet post processing: the mussel of planting after sheet is first placed in clear water reserviors and is recuperated, change clear water every day 1～3 time, carry out oxygen cycle simultaneously, maintenance oxygen concentration is 5～7mg/L, after 8～15 days, transfer to again the cultivation of freshwater aquiculture district,
Wherein the seawater shellfish in step (1) is selected from black lip shell, white butterfly shell or penguin shellfish, and mussel is selected from hydriopsis cumingii.
2. method according to claim 1, wherein in step (1), purified treatment is first to use the aureomycin solution rinsing 5～8 times of 500～1000U/mL, then uses the cobalt chloride solution rinsing 3～5 times of 100～125 μ mol/L.
3. method according to claim 1, the sheet pearly shellfish of planting of wherein transferring to the cultivation of freshwater aquiculture district in step (3) obtains the characteristic pearl with seawater conch feature afterwards cultivating 2-5.
4. method according to claim 1, the pearl of wherein cultivating is that the black with black lip shell pearl feature is pearl, has the white color system pearl of white butterfly shell pearl feature and have the bronze colour system pearl of penguin conch feature.
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| CN201310280086.3A CN103340168B (en) | 2013-07-03 | 2013-07-03 | Special fresh water pearl cultivating method based on small seawater shellfish cell pieces |
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| CN103340168B true CN103340168B (en) | 2014-09-03 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103598129B (en) * | 2013-12-02 | 2015-10-07 | 浙江海洋学院 | The breeding method of iris fresh water pearl |
| CN104756915B (en) * | 2015-04-09 | 2018-08-10 | 广东海洋大学 | A kind of breeding method of colorful sea water pearls |
| CN104957066B (en) * | 2015-07-09 | 2018-08-21 | 海南大学 | A kind of one three using Pteria penguin three type pearls of cultivation uses method |
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| CN101455187A (en) * | 2009-01-08 | 2009-06-17 | 梁盛 | Sea-water culturing method of pteria penguin bronze-coloured free pearls |
| CN101653103A (en) * | 2009-09-05 | 2010-02-24 | 湖南文理学院 | Breeding method of ultra-large nuclear-free pearl with good quality |
| CN102100193A (en) * | 2010-12-02 | 2011-06-22 | 广东海洋大学 | Method for breeding pearls by combining cell small pieces of black lip shells with pinctada martensii dunker |
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2013
- 2013-07-03 CN CN201310280086.3A patent/CN103340168B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5749319A (en) * | 1996-07-01 | 1998-05-12 | Hirose; Tokuzo | Pearl and method for producing same |
| CN1965644A (en) * | 2005-11-15 | 2007-05-23 | 施志仪 | Cultivation of nucleus pearl by using free cell implantation method |
| CN101019513A (en) * | 2006-09-01 | 2007-08-22 | 湖南文理学院 | Cultivation process of large no-kernel high quality pearl |
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Effective date of registration: 20211209 Address after: 536000 shop 102, blue coast community, No. 6, chating Road, Beihai City, Guangxi Zhuang Autonomous Region Patentee after: BEIHAI XIUPAI JEWELRY Co.,Ltd. Address before: 316000 No. 18 Haiyuan Road, Chengdong Street, Dinghai District, Zhoushan City, Zhejiang Province Patentee before: ZHEJIANG OCEAN University |