CN103340168A - Special fresh water pearl cultivating method based on small seawater shellfish cell pieces - Google Patents

Special fresh water pearl cultivating method based on small seawater shellfish cell pieces Download PDF

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Publication number
CN103340168A
CN103340168A CN2013102800863A CN201310280086A CN103340168A CN 103340168 A CN103340168 A CN 103340168A CN 2013102800863 A CN2013102800863 A CN 2013102800863A CN 201310280086 A CN201310280086 A CN 201310280086A CN 103340168 A CN103340168 A CN 103340168A
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shellfish
pearl
mussel
seawater
shell
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CN103340168B (en
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宋文东
纪丽丽
高良军
郭健
韩丝银
应知伟
王雅颖
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BEIHAI XIUPAI JEWELRY Co.,Ltd.
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Zhejiang Ocean University ZJOU
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention provides a special fresh water pearl cultivating method based on small seawater shellfish cell pieces. The pallium of a seawater shellfish is cut into small pieces, the small pieces are inserted in connective tissue of the pallium of a fresh water mussel, and a special pearl can be cultivated in fresh water after 2-3 years through processing.

Description

A kind of characteristic fresh water pearl breeding method based on seawater shellfish cell small pieces
Technical field
The invention belongs to the pearl culture field, especially the characteristic pearl cultivating method that combines of seawater shellfish and mussel.
Background technology
Pearl enjoys people's favor as the jewel queen always, and it divides from the source, two classes is arranged: seawater pearl and fresh water pearl.Fresh water pearl and seawater pearl are generally speaking more easy to identify, because seawater pearl color and luster is better than the fresh water pearl, and individual bigger than fresh water pearl, the shape circle.The seawater pearl is beautiful graceful, the sense that is translucent, and to a kind of soft dim U.S. of people, this is attributed to its gloss to a great extent, and the rounded probability of seawater pearl is than fresh water pearl height, and this also is to be determined by its inner concentric circular layer structure.The measured superior seawater pearl of matter is determined the price with gram, and its value can be considerably beyond gold.The fresh water pearl is as the living pearl of freshwater mussel, is being worth and poor qualitatively class than seawater pearl.Sea water pearls is mainly derived from shellfish kinds such as white butterfly shell, black lip shell, penguin shellfish and pteria martensii at present, first three plants the producing region mostly abroad, the main breed variety of China is pteria martensii, its pearl color is based on white, by contrast, external black lip shell and penguin shellfish respectively institute's black that produces and bronze-colored pearl price than the white pearl costliness of same grade a lot.Because China's freshwater mussel body survival rate height, and the freshwater aquiculture field is wide, therefore seawater shellfish black lip shell, penguin shellfish is combined with mussel respectively and cultivates the active demand that the characteristic pearl has been complied with China cultured pearl field just.Nowadays adopt the shellfish jacket membrane patch-graft interior to cultivate the method for pearl to the pearly shellfish body, it is the main technique of artificial culture pearl always, at present this method is grown cultured pearls in the field separately seawater shellfish and mussel and is all obtained some successes, implants pan colour system pearl in back in the hydriopsis cumingii body as the small pieces that cristaria plicata is produced; The small pieces that the circle anodonta woodiana pacifica is produced are implanted product pink pearl in back in the cristaria plicata body; Producing black after the small pieces that black lip shell is produced are implanted in the pteria martensii body is pearl; Yet this method is applied to the seawater shellfish and mussel yet there are no report in conjunction with the field, therefore seawater shellfish black lip shell, penguin shellfish are combined with mussel respectively and cultivate the characteristic pearl, and in freshwater aquiculture, enlarge production, be the new breakthrough in China cultured pearl field.
Summary of the invention
The objective of the invention is to provide a kind of at characteristic seawater pearl in China's output problem on the low side seawater shellfish and mussel are combined the method for cultivating the characteristic pearl.It is black lip shell, white butterfly shell and penguin shellfish that the present invention is suitable for the seawater shellfish, and mussel is hydriopsis cumingii, and the black that its characteristic is to cultivate black lip shell pearl feature is the bronze colour system pearl of pearl and penguin conch feature.
For achieving the above object, the present invention adopts following technical scheme:
(1), selection and the film-making of piece shell: selecting the seawater shellfish is black lip shell, white butterfly shell and penguin shellfish, and the shellfish body is intact, and the age, body was grown 4~6cm at 1~2 years old.Take off the outer embrane purified treatment from the seawater shellfish, require earlier with aureomycin solution rinsing 5~8 times, the aureomycin solution concentration is 500~1000U/mL, use the cobalt chloride solution rinsing again 3~5 times, cobalt chloride solution concentration is 100~125 μ mol/L, the section back in time drips the tissue juice maintenance of former shellfish, require small pieces thickness to be: 0.5~0.8mm, size specification is: 1.5~3mm * 1.5~3mm, the film-making process is finished in 1~4min, and in time be used for inoculation, can not deposit too of a specified duration, generally in 0.5~2.5 hour inoculation for good.
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in the mussel as pearly shellfish, require the freshwater mussel body intact, the seedless mussel age, the long 4~6cm of body had the nuclear mussel age at 1.5~2.5 years old at 0.5~1 years old, the long 6~8cm of body.The made small pieces of step (1) are inserted in the pallial connective tissue of mussel.
(3) plant the sheet post processing: will plant mussel behind the sheet and be placed in the clear water reserviors earlier and recuperate, change clear water every day to keep water cleaning, as changing clear water every day 1~3 time, carry out oxygen cycle simultaneously, the maintenance oxygen concentration is 5~7mg/L, transfers to the freshwater aquiculture district after 8~15 days again and cultures.
Advantage of the present invention: seawater shellfish and mussel are simple and feasible in conjunction with the method for cultivating the characteristic pearl, have not only improved the value of existing pearl, and have been suitable for a large amount of productions.
Embodiment
Below by specific embodiment technical scheme of the present invention is made concrete exemplary illustration, be not construed as limiting the invention.
Embodiment 1:
(1), selection and the film-making of piece shell: select black lip shell, the shellfish body is intact, 2 years old age, the long 5cm of body; Take off outer embrane from black lip shell and carry out purified treatment, earlier with the aureomycin solution rinsing of 800U/mL 7 times, use the cobalt chloride solution rinsing 5 times of 125 μ mol/L again; The tissue juice of outer embrane after the purified treatment being cut and dripped into pieces former shellfish maintains, and small pieces thickness is 0.6mm, and size specification is: 3mm * 3mm, and whole film-making process is finished with 2.5min;
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in the mussel as pearly shellfish, seedless, intact, 1 years old age, the long 6cm of body is inserted into the made small pieces of step (1) in the pallial connective tissue of hydriopsis cumingii in 1 hour after section is finished;
(3), plant the sheet post processing: will plant hydriopsis cumingii behind the sheet and be placed in the clear water reserviors earlier and recuperate, change clear water every day one time, carry out oxygen cycle simultaneously, the maintenance oxygen concentration is 6mg/L, transferring to the freshwater aquiculture district after 14 days again cultures, cultivate after 3 years, producing the black with black lip shell pearl feature is pearl.
Embodiment 2:
(1), selection and the film-making of piece shell: select white butterfly shell, the shellfish body is intact, 1.5 years old age, the long 4cm of body; Take off outer embrane from white butterfly shell and carry out purified treatment, earlier with the aureomycin solution rinsing of 1000U/mL 6 times, use the cobalt chloride solution rinsing 4 times of 125 μ mol/L again; The tissue juice of outer embrane after the purified treatment being cut and dripped into pieces former shellfish maintains, and small pieces thickness is 0.6mm, and size specification is: 3mm * 3mm, and whole film-making process is finished with 2.5min;
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in the mussel as pearly shellfish, seedless, intact, 0.5 years old age, the long 5cm of body is inserted into the made small pieces of step (1) in the pallial connective tissue of hydriopsis cumingii in 0.5 hour after section is finished;
(3), plant the sheet post processing: will plant hydriopsis cumingii behind the sheet and be placed in the clear water reserviors earlier and recuperate, change clear water every day one time, carry out oxygen cycle simultaneously, the maintenance oxygen concentration is 6mg/L, transferring to the freshwater aquiculture district after 14 days again cultures, cultivate after 4 years, produce the white color system pearl with white butterfly shell pearl feature.
Embodiment 3:
(1), selection and the film-making of piece shell: select the penguin shellfish, the shellfish body is intact, 1.5 years old age, the long 5cm of body; Take off outer embrane from the penguin shellfish and carry out purified treatment, earlier with the aureomycin solution rinsing of 1000U/mL 6 times, use the cobalt chloride solution rinsing 5 times of 100 μ mol/L again; The tissue juice of outer embrane after handling being cut and dripped into pieces former shellfish maintains, and small pieces thickness is 0.6mm, and size specification is: 3mm * 3mm, and whole film-making process is finished with 3min;
(2), the selection of pearly shellfish and plant sheet: select hydriopsis cumingii in the mussel as pearly shellfish, nuclear is arranged, intact, 2 years old age, the long 7cm of body is inserted into the made small pieces of step (1) in the pallial connective tissue of hydriopsis cumingii in 0.5 hour after section is finished;
(3), plant the sheet post processing: will plant hydriopsis cumingii behind the sheet and be placed in the clear water reserviors earlier and recuperate, change clear water every day one time, carry out oxygen cycle simultaneously, the maintenance oxygen concentration is 6mg/L, transferring to the freshwater aquiculture district after 14 days again cultures, cultivate after 2 years, produce the bronze colour system pearl with penguin conch feature.

Claims (5)

1. characteristic fresh water pearl breeding method based on seawater shellfish mantle piece may further comprise the steps:
(1) selection of piece shell and film-making: selecting the shellfish body is the seawater shellfish, intact, age was at 1~2 years old, long 4~the 6cm of body, take off outer embrane from the seawater shellfish and carry out purified treatment, the tissue juice of the outer embrane after the purified treatment being cut and dripped into pieces former shellfish maintains, and requiring small pieces thickness is 0.5~0.8mm, size specification is 1.5~3mm * 1.5~3mm, and the film-making process is finished in 1~4min;
(2) selection of pearly shellfish and plant sheet: select the shellfish body to be mussel, intact, the seedless mussel age, the long 4~6cm of body had the nuclear mussel age at 1.5~2.5 years old at 0.5~1 years old, the long 6~8cm of body; After film-making is finished, be inserted into the made small pieces of step (1) in the pallial connective tissue of mussel in 0.5~2.5 hour;
(3) plant the sheet post processing: will plant mussel behind the sheet and be placed in the clear water reserviors earlier and recuperate, and change clear water every day 1~3 time, and carry out oxygen cycle simultaneously, the maintenance oxygen concentration is 5~7mg/L, transfers to the freshwater aquiculture district after 8~15 days again and cultures.
2. method according to claim 1, wherein the seawater shellfish in the step (1) is selected from black lip shell, white butterfly shell or penguin shellfish, and mussel is selected from hydriopsis cumingii.
3. method according to claim 1 and 2, wherein purified treatment is earlier with the aureomycin solution rinsing of 500~1000U/mL 5~8 times in the step (1), uses the cobalt chloride solution rinsing 3~5 times of 100~125 μ mol/L again.
4. according to each described method among the claim 1-3, the sheet pearly shellfish of planting of transferring in the step (3) wherein that the freshwater aquiculture district cultures obtains characteristic pearl with seawater conch feature after cultivating 2-5.
5. according to each described method among the claim 1-4, the pearl of wherein cultivating is that the black with black lip shell pearl feature is pearl, the bronze colour system pearl that has the white color system pearl of white butterfly shell pearl feature and have penguin conch feature.
CN201310280086.3A 2013-07-03 2013-07-03 Special fresh water pearl cultivating method based on small seawater shellfish cell pieces Active CN103340168B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103598129A (en) * 2013-12-02 2014-02-26 浙江海洋学院 Method for cultivating iridescence fresh water pearls
CN104756915A (en) * 2015-04-09 2015-07-08 广东海洋大学 Cultivation method of colorful seawater pearls
CN104957066A (en) * 2015-07-09 2015-10-07 海南大学 One-body-three-use method for cultivating three types of pearls by means of pteria penguin

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* Cited by examiner, † Cited by third party
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US5749319A (en) * 1996-07-01 1998-05-12 Hirose; Tokuzo Pearl and method for producing same
CN1965644A (en) * 2005-11-15 2007-05-23 施志仪 Cultivation of nucleus pearl by using free cell implantation method
CN101019513A (en) * 2006-09-01 2007-08-22 湖南文理学院 Cultivation process of large no-kernel high quality pearl
CN101036451A (en) * 2007-04-24 2007-09-19 詹伟建 Systematized disinfecting method for nucleus-insertion technique of nucleated pearl
CN101036450A (en) * 2007-04-24 2007-09-19 詹伟建 Pearl sacs assistant formation method after nucleus-insertion
CN101317557A (en) * 2008-07-02 2008-12-10 王卓锋 Disinfection method for operation system for planting nucleus in pearl mussel having nucleus
CN101352154A (en) * 2008-09-12 2009-01-28 海南大学 Method for processing mantle piece and improving quality of sea water pearl
CN101455187A (en) * 2009-01-08 2009-06-17 梁盛 Sea-water culturing method of pteria penguin bronze-coloured free pearls
CN101653103A (en) * 2009-09-05 2010-02-24 湖南文理学院 Breeding method of ultra-large nuclear-free pearl with good quality
CN102100193A (en) * 2010-12-02 2011-06-22 广东海洋大学 Method for breeding pearls by combining cell small pieces of black lip shells with pinctada martensii dunker

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5749319A (en) * 1996-07-01 1998-05-12 Hirose; Tokuzo Pearl and method for producing same
CN1965644A (en) * 2005-11-15 2007-05-23 施志仪 Cultivation of nucleus pearl by using free cell implantation method
CN101019513A (en) * 2006-09-01 2007-08-22 湖南文理学院 Cultivation process of large no-kernel high quality pearl
CN101036451A (en) * 2007-04-24 2007-09-19 詹伟建 Systematized disinfecting method for nucleus-insertion technique of nucleated pearl
CN101036450A (en) * 2007-04-24 2007-09-19 詹伟建 Pearl sacs assistant formation method after nucleus-insertion
CN101317557A (en) * 2008-07-02 2008-12-10 王卓锋 Disinfection method for operation system for planting nucleus in pearl mussel having nucleus
CN101352154A (en) * 2008-09-12 2009-01-28 海南大学 Method for processing mantle piece and improving quality of sea water pearl
CN101455187A (en) * 2009-01-08 2009-06-17 梁盛 Sea-water culturing method of pteria penguin bronze-coloured free pearls
CN101653103A (en) * 2009-09-05 2010-02-24 湖南文理学院 Breeding method of ultra-large nuclear-free pearl with good quality
CN102100193A (en) * 2010-12-02 2011-06-22 广东海洋大学 Method for breeding pearls by combining cell small pieces of black lip shells with pinctada martensii dunker

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103598129A (en) * 2013-12-02 2014-02-26 浙江海洋学院 Method for cultivating iridescence fresh water pearls
CN104756915A (en) * 2015-04-09 2015-07-08 广东海洋大学 Cultivation method of colorful seawater pearls
CN104756915B (en) * 2015-04-09 2018-08-10 广东海洋大学 A kind of breeding method of colorful sea water pearls
CN104957066A (en) * 2015-07-09 2015-10-07 海南大学 One-body-three-use method for cultivating three types of pearls by means of pteria penguin

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