A kind of method of domestically culturing salt-water Spirulina
Technical field
What the present invention relates to is a kind of method of culturing spirulina, relate in particular to a kind of can be popular, the method for the domestically culturing salt-water Spirulina of generalization and simplification.
Background technology
Spirulina ownership Cyanophyta, Cyanophyceae, disconnected body order, Oscillariaceae, the Spirullina of growing, it is the phycophyta that a kind of cell physiological result is similar to bacterium, be blackish green, it is a kind of photosynthetic autotrophs biology, be the abundantest, the isostatic natural nutrition subsidy food of the nutrition found up to now, it contains human daily necessary protein, and constituting proteinic amino acid composition is in admirable proportion, also some human body is difficult to the composition of acquisition from other food, and its digestion is up to 95%, very easily by human consumption and absorption.The spirulina product has been applied to human edible field at present, and spirulina has anti-virus ability preferably, and effectively lowering blood glucose, cholesterol are a kind of higher medical health care products that is worth.Traditional spirulina all adopts freshwater aquiculture or sea farming, be called fresh water spirulina or seawater spirulina, these two kinds of breed modes, the open-air formula of basic employing is cultured, utilize natural lake or build large-scale breed pond and culture, the advantage of this breeding way is that cost is low, and shortcoming is that quality product is difficult to guarantee, unit output is lower, is subjected to the influence in environment and season easily.Such industrialized culture, culture the spirulina that comes out and just can become the edible food of people through industrialized processing again, in the breed and the course of processing, can cause the loss of nutrition unavoidably or bring impurity into, thereby reduce the quality and the trophic function of spirulina.And the family-raise spirulina still belongs to the field that technical progress owes to pay close attention at present, the family-raise spirulina just looks like to breed fish in the family, grow flower equally, can beautify home environment on the one hand, the fresh spirulina that can obtain quality-guarantee on the other hand is edible, to replenish protein.By retrieval, find that publication number is CN1254012, title is the patent application of " a kind of method of domestic edible fresh Spirulina ", discloses a kind of method of domestic spirulina, though this method has the characteristics of himself, but used nutrient solution prescription is complicated, raw materials used have a kind more than 20, and cultural method is cumbersome, and step is too many, simple with cultural method, the requirement of implementing greatly differs from each other easily.Therefore, providing a kind of method of effective simple and practical family-raise spirulina is highly significant.
Summary of the invention
The method that the purpose of this invention is to provide a kind of domestically culturing salt-water Spirulina, be subject to the influence in environment and season, bring the deficiency of impurity into easily to overcome the industrialized culture spirulina, thus the spirulina breeding method is become be not subject to ectocine, controlled, having can successional cultural method.
In order to reach above purpose, the present invention adopts following technical scheme, and a kind of method of domestically culturing salt-water Spirulina may further comprise the steps:
(1) it is standby to get sodium bicarbonate 8.0-13.9 gram, dipotassium hydrogen phosphate 0.1-0.5 gram, SODIUMNITRATE 2.1-2.8 gram, vitriolate of tartar 0.8-1.2 gram, sal epsom 0.1-0.5 gram, calcium chloride 0.01-0.05 gram, ferric sulfate 0.01-0.05 gram, disodium ethylene diamine tetraacetate 0.05-0.08 gram, sodium-chlor 3.6-4.2 gram, boric acid 0.01-0.09 gram, Manganous chloride tetrahydrate 0.01-0.05 gram, zinc sulfate 0.01-0.05 gram, titanium sulfate 0.01-0.03 gram and Xiao Suangu 0.01-0.05 gram;
(2) sodium bicarbonate in the aforementioned proportion prescription, dipotassium hydrogen phosphate, SODIUMNITRATE, vitriolate of tartar, sal epsom, calcium chloride, ferric sulfate, disodium ethylene diamine tetraacetate and sodium-chlor are added mixing in the entry in order, and in adition process, do not stop to stir, till dissolving; Again boric acid, Manganous chloride tetrahydrate, zinc sulfate, titanium sulfate, Xiao Suangu are sequentially added into mixing in the water, and in adition process, do not stop to stir, till dissolving; Above-mentioned two kinds of solution are mixed and stir, and water is adjusted to 1 liter, leave standstill, getting supernatant liquor and transferring pH value is 8.5-10, and it is standby to obtain nutrient solution;
(3) amount of pressing the 3-8 grams per liter in above-mentioned nutrient solution adds spirulina algae kind, stirs, and the algae kind was uniformly distributed in the culturing liquid development growth 2-8 days, during water temperature remain on 18 ℃-38 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 6-18 hour;
(4) spirulina reaches ripe after above-mentioned breed, the string bag with 200-300 order filtering net is gathered, ripe spirulina is stayed on the filtering net, still immature spirulina then flows into again in company with culturing liquid by filtering net and repeats said process in the Cultivation container again, so far ripe spirulina is separated with the prematurity spirulina, ripe spirulina is edible, and the prematurity spirulina is uninterrupted continuously cultures.
Taking the present invention of above-mentioned measure, is a kind of method of suitable domestically culturing salt-water Spirulina, and the spirulina that breed is come out after testing, it is many to have the spiral number, and filament is longer, and cell is big, the advantage that cell walls is more loose, so be absorbed by the body easily, the dietetic alimentation rate is up to the characteristics more than 90%.And cultural method is easy, and cleaning easily.Culture spirulina with the present invention, breeding environment can be located at indoor, therefore can reduce the pollution of environment, carry out aseptic technique, the content of its trace element components is abundant and balanced than the fresh water spirulina, contains than the more active factor of fresh water spirulina, spirulina with the present invention's breed, after testing, contain active substance polysaccharides, inositol and Phycocyanins, C-, and the content of protein, Y-linolenic acid and Phycocyanins, C-is all higher.Culture spirulina with the present invention, can also keep the pure of spirulina algae kind.Culture spirulina with the present invention, can promote spirulina growth, shorten the culture-cycle of spirulina, through experiment, get 250 ml flasks and inoculate 200 milliliters of algae liquid in proportion, every day, timing sampling was counted cell count at microscopically, obtained following experimental data:
Fate |
The spirulina that the present invention cultures |
The spirulina that common cultural method is cultured |
??0 |
??3.33E+06 |
??3.40E+06 |
??1 |
??7.49E+06 |
??8.30E+06 |
??2 |
??1.88E+07 |
??1.42E+07 |
??3 |
??3.06E+07 |
??1.58E+07 |
??4 |
??4.90E+07 |
??4.22E+07 |
??5 |
??5.18E+07 |
??5.15E+07 |
??6 |
??6.66E+07 |
??4.98E+07 |
By above-mentioned experimental data as can be known, with the spirulina that the present invention cultures, the peak period that entered vegetative period since the 2nd day, and the spirulina of culturing with common cultural method enters the summit of growth phase since the 4th talent.Prompting thus, the spirulina with the present invention cultures has obviously shortened growth cycle, has significantly reduced aquaculture cost.
Embodiment
Embodiment 1
1, get prescription:
Sodium bicarbonate 800g dipotassium hydrogen phosphate 10g SODIUMNITRATE 210g vitriolate of tartar 80g
Sodium-chlor 360g sal epsom 10g calcium chloride 1g ferric sulfate 1g
Manganous chloride tetrahydrate 1g zinc sulfate 1g titanium sulfate 1g Xiao Suangu 1g
5g is standby for boric acid 1g disodium ethylene diamine tetraacetate;
2, sodium bicarbonate, dipotassium hydrogen phosphate, SODIUMNITRATE, vitriolate of tartar, sal epsom, calcium chloride, ferric sulfate, disodium ethylene diamine tetraacetate and the sodium-chlor of getting in the above-mentioned prescription adds mixing in 80 premium on currency in order, in adition process, do not stop to stir, till dissolving; Boric acid, Manganous chloride tetrahydrate, zinc sulfate, titanium sulfate, the Xiao Suangu got in the above-mentioned prescription are sequentially added into mixing in 15 premium on currency again, and do not stop to stir in adition process, till dissolving; Above-mentioned two kinds of solution are mixed and stirs, and water is adjusted to 100 liters, leave standstill, supernatant liquor and to transfer pH value to obtain nutrient solution to 8-10 standby;
3, in above-mentioned nutrient solution, add 300 gram spirulina algae kinds, stir, make the algae kind be uniformly distributed in the culturing liquid development growth 7 days, during water temperature remain on 20-25 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 6 hours;
4, spirulina reaches ripe after above-mentioned breed, the string bag with 300 order filtering nets is gathered, ripe spirulina is stayed on the filtering net, still immature spirulina then flows into again in company with culturing liquid by filtering net and repeats said process in the Cultivation container again, so far ripe spirulina is separated with the prematurity spirulina, ripe spirulina is edible, and the prematurity spirulina can uninterruptedly be cultured under the prerequisite of in good time replenishing nutrient solution continuously.
Embodiment 2
1, get prescription:
Sodium bicarbonate 1300g dipotassium hydrogen phosphate 50g SODIUMNITRATE 280g vitriolate of tartar 120g
Sodium-chlor 420g sal epsom 50g calcium chloride 5g ferric sulfate 5g
Manganous chloride tetrahydrate 5g zinc sulfate 5g titanium sulfate 3g Xiao Suangu 5g
8g is standby for boric acid 9g disodium ethylene diamine tetraacetate;
2, the step 2 with embodiment 1 is identical;
3, in above-mentioned nutrient solution, add 800 gram spirulina algae kinds, stir, make the algae kind be uniformly distributed in the culturing liquid development growth 5 days, during water temperature remain on 20-25 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 12 hours;
4, the step 4 with embodiment 1 is identical.
Embodiment 3
1, get and join ten thousand:
Sodium bicarbonate 890g dipotassium hydrogen phosphate 50g SODIUMNITRATE 250g vitriolate of tartar 100g
Sodium-chlor 420g sal epsom 20g calcium chloride 2g ferric sulfate 1g
Manganous chloride tetrahydrate 1g zinc sulfate 2g titanium sulfate 3g Xiao Suangu 1g
8g is standby for boric acid 1g disodium ethylene diamine tetraacetate;
2, the step 2 with embodiment 1 is identical;
3, in above-mentioned nutrient solution, add 500 gram spirulina algae kinds, stir, make the algae kind be uniformly distributed in the culturing liquid development growth 7 days, during water temperature remain on 20-25 ℃, oxygenation 24 hours every days also guarantees that every day, light application time reached 18 hours;
4, the step 4 with embodiment 1 is identical.