CN104017762A - Culture method of spirulina - Google Patents
Culture method of spirulina Download PDFInfo
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- CN104017762A CN104017762A CN201410291389.XA CN201410291389A CN104017762A CN 104017762 A CN104017762 A CN 104017762A CN 201410291389 A CN201410291389 A CN 201410291389A CN 104017762 A CN104017762 A CN 104017762A
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Abstract
The invention discloses a culture method of spirulina, which comprises the following steps: culture tank selection: selecting a pollution-free culture tank, and regulating the temperature and illumination intensity of the culture tank; adding a culture solution into the culture tank, adding spirulina seeds, and culturing; adding sufficient water into the culture tank, uniformly mixing the culture solution and water in a volume ratio of (1-10):800, controlling the pH value of water in the culture tank at 8.6-9.5, and stirring the spirulina seeds to enable the spirulina seeds to perform normal photosynthesis; after the spirulina is ripe, and collecting with a 400-mesh filter screen, wherein the ripe spirulina stays on the filter screen, and the unripe spirulina flows into the culture tank through the holes of the filter screen. The method is environment-friendly and ecological, increases the survival percent of the seedlings, enhances the disease tolerance of the seedlings, obviously lowers the mortality of the seedlings, and improves the texture and mouthfeel of the finished product.
Description
Technical field
The present invention relates to cultural technique field, be specifically related to a kind of cultural method of spirulina.
Background technology
Spirulina is a class unicellular lower eukaryote, and prokaryotic organism, by filament unicellular or that many cells form, the long 200-500 μ of body m, wide 5-10 μ m, cylindrical, be loose or well-regulated spiral rotary shape bending closely, shape is as clockwork feed, so gain the name, Spirullina Cyanophyta, section is grown order, Oscillariaceae, Spirullina, outward appearance is green, microscope curl.The spirulina growth history of existing 3,500,000,000 years, is on the earth, to carry out the earliest one of photosynthetic prokaryotic organism, and vitality is extremely strong; Spirullina many cells frond, cylindrical spiral helicine filament, single raw or cluster consor, algal filament diameter 5-10 μ m, tip obtuse, spiral is counted 2-7, frond can tremble and rotatablely move, and often, as rotation very soon round a longitudinal axis, creeps forward, cell content is even, without real nucleus.Due to the different amts of the phycoerythrobilin in body and algocyan etc., and present different body colours, as blue-greenish colour, yellow-green colour or red-purple etc.And there is a slim and frahile cross wall.Belong to procaryotic simple modes of reproduction, can amitosis.
At present natural yield of spirulina is very limited, and the output of the synthetic of having taked can not meet people's demand, therefore needs a kind of method of producing spirulina effectively to improve the output of spirulina.
Summary of the invention
Object of the present invention provides a kind of cultural method of spirulina, solves one or more in above-mentioned prior art problem.
According to an aspect of the present invention, a kind of cultural method of spirulina, comprises the following steps:
The selection of S1, culturing pool, selects free of contamination culturing pool, temperature and the intensity of illumination of regulation and control culturing pool;
S2, in culturing pool, add nutrient solution, then put into spirulina algae kind and cultivate;
S3, in culturing pool, adding enough water, is 1～10:800 according to the volume ratio of nutrient solution and water, mixes, and controlling culturing pool pond water pH value is 8.6～9.5, and stirring spirulina algae kind, allows spirulina algae kind carry out normal photosynthesis;
It is ripe that S4, spirulina reach after above-mentioned cultivation, with 400 order filtering nets, collects, and ripe spirulina is stayed on filtering net, and immature spirulina flows in culturing pool by filtering net net hole, continues to cultivate.
The cultural method of a kind of spirulina provided by the invention, has following advantage:
1, environmental protection, ecological free from environmental pollution, sustainable use;
2, more than improving the surviving rate to 60% of Seedling Stage seedling;
3, the disease resistance that improves Seedling Stage seedling, obviously reduces the mortality ratio of Seedling Stage seedling;
4, improve meat and the mouthfeel of finished product.
In some embodiments, the temperature of culturing pool is controlled at 15 ℃～35 ℃, and intensity of illumination is 30000～35000 lux/skies, and incubation time is 12～24h.
In some embodiments, nutrient solution comprises sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, zinc sulfate and manganous sulfate.Sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, the concentration of zinc sulfate and manganous sulfate is respectively the sodium bicarbonate of 2～4g/L, the sodium-chlor of 0.01～0.06g/L, the Repone K of 0.25～0.85g/L, the calcium chloride of 0.05～0.1g/L, the dipotassium hydrogen phosphate of 0.15～0.25g/L, the sodium carbonate of 0.01～0.06g/L, the potassium sulfate of 0.04～0.2g/L, the SODIUMNITRATE of 0.06～0.08g/L, the magnesium sulfate of 0.1～0.6g/L, the phosphoric acid of 0.2～0.75g/L, the ferrous sulfate of 0.05～0.15g/L, the boric acid of 0.03～0.08g/L, the zinc sulfate of 0.05～0.25g/L and the manganous sulfate of 0.03～0.06g/L.
| The name of an article | Source |
| Sodium bicarbonate | The Hua Xinjin of Shenzhen profit Chemical Industry Science Co., Ltd model: X3000 |
| Sodium-chlor | Hangzhou Yong Xingwujiao company limited rank: analytical pure AR |
| Repone K | Lianyungang Chemical Co., Ltd. of TongYuan model: GB25585 ?2010 |
| Calcium chloride | Lianyun Harbour Run Tai Chemical Co., Ltd. model: anhydrous |
| Dipotassium hydrogen phosphate | Lianyungang Chemical Co., Ltd. of TongYuan model: GB16788 ?57 ?1 |
| Sodium carbonate | Lianyungang Chemical Co., Ltd. of TongYuan model: 40 kilograms |
| Potassium sulfate | Lianyungang Chemical Co., Ltd. of TongYuan model: AR7778 ?80 ?5 |
| SODIUMNITRATE | Lianyungang Chemical Co., Ltd. of TongYuan model: GB2367 ?2006 |
| Magnesium sulfate | Lianyungang Chemical Co., Ltd. of TongYuan model: HG/T2680 ?2009 |
| Phosphoric acid | Lianyungang Chemical Co., Ltd. of TongYuan model: GB/T2091 ?08 |
| Ferrous sulfate | Lianyungang Chemical Co., Ltd. of TongYuan model: CAS7720 ?78 ?7 |
| Boric acid | Lianyungang Chemical Co., Ltd. of TongYuan model: GB/T538 ?06 |
| Zinc sulfate | Lianyungang Chemical Co., Ltd. of TongYuan model: CAS:CCC |
| Manganous sulfate | Lianyungang Chemical Co., Ltd. of TongYuan model: NY/T1111 ?2006 |
Embodiment
Embodiment 1
A cultural method for spirulina, comprises the following steps:
The selection of S1, culturing pool, selects free of contamination culturing pool, in culturing pool, adds nutrient solution;
The temperature of S2, regulation and control culturing pool is 15 ℃, intensity of illumination is 30000 lux/skies, incubation time is 12h, light application time is 8 hours, nutrient solution premodulation 100L, nutrient solution comprises sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, zinc sulfate and manganous sulfate;
Sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, the concentration of zinc sulfate and manganous sulfate is respectively the sodium bicarbonate of 2g/L, the sodium-chlor of 0.01g/L, the Repone K of 0.25g/L, the calcium chloride of 0.05g/L, the dipotassium hydrogen phosphate of 0.15g/L, the sodium carbonate of 0.01g/L, the potassium sulfate of 0.04g/L, the SODIUMNITRATE of 0.06g/L, the magnesium sulfate of 0.1g/L, the phosphoric acid of 0.2g/L, the ferrous sulfate of 0.05g/L, the boric acid of 0.03g/L, the zinc sulfate of 0.05g/L and the manganous sulfate of 0.03g/L, then putting into spirulina algae kind cultivates,
S3, in culturing pool, add enough water, according to the volume ratio of nutrient solution and water, be 1:800, mix, to the sodium hydrogen carbonate solution that adds 5% in culturing pool, regulate and control pH value, culturing pool pH value is 8.6, stir spirulina algae kind, agitation speed is 100r/min, allows spirulina algae kind carry out normal photosynthesis;
It is ripe that S4, spirulina reach after above-mentioned cultivation, and body reaches 400 μ m, and wide 8 μ m just can collect above, with 400 order filtering nets, collect, ripe spirulina is stayed on filtering net, and immature spirulina flows in culturing pool by filtering net net hole, continues to cultivate.
Step 3 is spirulina Seedling Stage, and the seedling percent of spirulina Seedling Stage is 61.3%, and death of seedling rate is 38.7%.
Embodiment 2
A cultural method for spirulina, comprises the following steps:
The selection of S1, culturing pool, selects free of contamination culturing pool, in culturing pool, adds nutrient solution;
The temperature of S2, regulation and control culturing pool is 25 ℃, intensity of illumination is 32000 lux/skies, incubation time is 18h, light application time is 10 hours, nutrient solution premodulation 100L, nutrient solution comprises sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, zinc sulfate and manganous sulfate.
Sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, the concentration of zinc sulfate and manganous sulfate is respectively the sodium bicarbonate of 3g/L, the sodium-chlor of 0.03g/L, the Repone K of 0.6g/L, the calcium chloride of 0.08g/L, the dipotassium hydrogen phosphate of 0.2g/L, the sodium carbonate of 0.03g/L, the potassium sulfate of 0.1g/L, the SODIUMNITRATE of 0.07g/L, the magnesium sulfate of 0.4g/L, the phosphoric acid of 0.5g/L, the ferrous sulfate of 0.1g/L, the boric acid of 0.06g/L, the zinc sulfate of 0.15g/L and the manganous sulfate of 0.045g/L, then putting into spirulina algae kind cultivates,
S3, in culturing pool, add enough water, according to the volume ratio of nutrient solution and water, be 6:800, mix, to the sodium hydrogen carbonate solution that adds 5% in culturing pool, regulate and control pH value, culturing pool pH value is 9, stir spirulina algae kind, agitation speed is 100r/min, allows spirulina algae kind carry out normal photosynthesis;
It is ripe that S4, spirulina reach after above-mentioned cultivation, and body reaches 400 μ m, and wide 8 μ m just can collect above, with 400 order filtering nets, collect, ripe spirulina is stayed on filtering net, and immature spirulina flows in culturing pool by filtering net net hole, continues to cultivate.
Step 3 is spirulina Seedling Stage, and the seedling percent of spirulina Seedling Stage is 64.7%, and death of seedling rate is 35.3%.
Embodiment 3
A cultural method for spirulina, comprises the following steps:
The selection of S1, culturing pool, selects free of contamination culturing pool, in culturing pool, adds nutrient solution;
The temperature of S2, regulation and control culturing pool is 35 ℃, intensity of illumination is 35000 lux/skies, incubation time is 24h, light application time is 12 hours, nutrient solution premodulation 100L, nutrient solution comprises sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, zinc sulfate and manganous sulfate.
Sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, the concentration of zinc sulfate and manganous sulfate is respectively the sodium bicarbonate of 4g/L, the sodium-chlor of 0.06g/L, the Repone K of 0.85g/L, the calcium chloride of 0.1g/L, the dipotassium hydrogen phosphate of 0.25g/L, the sodium carbonate of 0.06g/L, the potassium sulfate of 0.2g/L, the SODIUMNITRATE of 0.08g/L, the magnesium sulfate of 0.6g/L, the phosphoric acid of 0.75g/L, the ferrous sulfate of 0.15g/L, the boric acid of 0.08g/L, the zinc sulfate of 0.25g/L and the manganous sulfate of 0.06g/L, then putting into spirulina algae kind cultivates,
S3, in culturing pool, add enough water, according to the volume ratio of nutrient solution and water, be 10:800, mix, to the sodium hydrogen carbonate solution that adds 5% in culturing pool, regulate and control pH value, culturing pool pH value is 9.5, stir spirulina algae kind, agitation speed is 100r/min, allows spirulina algae kind carry out normal photosynthesis;
It is ripe that S4, spirulina reach after above-mentioned cultivation, and body reaches 400 μ m, and wide 8 μ m just can collect above, with 400 order filtering nets, collect, ripe spirulina is stayed on filtering net, and immature spirulina flows in culturing pool by filtering net net hole, continues to cultivate.
Step 3 is spirulina Seedling Stage, and the seedling percent of spirulina Seedling Stage is 64.3%, and death of seedling rate is 35.7%.
The above is only optimal way of the present invention; it should be pointed out that to those skilled in the art, without departing from the concept of the premise of the invention; can also make some similar distortion and improvement, within these also should be considered as protection scope of the present invention.
Claims (4)
1. a cultural method for spirulina, is characterized in that, comprises the following steps:
The selection of S1, culturing pool, selects free of contamination culturing pool, in culturing pool, adds nutrient solution;
Temperature and the intensity of illumination of S2, regulation and control culturing pool, then put into spirulina algae kind and cultivate;
S3, in culturing pool, adding enough water, is 1～10:800 according to the volume ratio of nutrient solution and water, mixes, and controlling culturing pool pond water pH value is 8.6～9.5, and stirring spirulina algae kind, allows spirulina algae kind carry out photosynthesis;
It is ripe that S4, spirulina reach after above-mentioned cultivation, with 400 order filtering nets, collects, and ripe spirulina is stayed on filtering net, and immature spirulina flows in culturing pool by filtering net net hole, continues to cultivate.
2. the cultural method of a kind of spirulina according to claim 1, is characterized in that, the temperature of described culturing pool is controlled at 15 ℃～35 ℃, and intensity of illumination is 30000～35000 lux/skies, and incubation time is 12～24h.
3. the cultural method of a kind of spirulina according to claim 1, it is characterized in that, described nutrient solution comprises sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, zinc sulfate and manganous sulfate.
4. the cultural method of a kind of spirulina according to claim 3, it is characterized in that, described sodium bicarbonate, sodium-chlor, Repone K, calcium chloride, dipotassium hydrogen phosphate, sodium carbonate, potassium sulfate, SODIUMNITRATE, magnesium sulfate, phosphoric acid, ferrous sulfate, boric acid, the concentration of zinc sulfate and manganous sulfate is respectively the sodium bicarbonate of 2～4g/L, the sodium-chlor of 0.01～0.06g/L, the Repone K of 0.25～0.85g/L, the calcium chloride of 0.05～0.1g/L, the dipotassium hydrogen phosphate of 0.15～0.25g/L, the sodium carbonate of 0.01～0.06g/L, the potassium sulfate of 0.04～0.2g/L, the SODIUMNITRATE of 0.06～0.08g/L, the magnesium sulfate of 0.1～0.6g/L, the phosphoric acid of 0.2～0.75g/L, the ferrous sulfate of 0.05～0.15g/L, the boric acid of 0.03～0.08g/L, the zinc sulfate of 0.05～0.25g/L and the manganous sulfate of 0.03～0.06g/L.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106011000A (en) * | 2016-05-23 | 2016-10-12 | 邹城市洪石泉螺旋藻生物科技有限公司 | Preparation method of iron-rich instant spirulina |
| CN108315274A (en) * | 2017-12-25 | 2018-07-24 | 内蒙古乌审召生态产业发展有限公司 | A kind of spirulina breeding method and system |
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| US20120021494A1 (en) * | 2010-07-23 | 2012-01-26 | Krebs & Sisier L.P. | Enhanced photosynthesis and photocatalysis water treatment/biomass growth process |
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Patent Citations (3)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106011000A (en) * | 2016-05-23 | 2016-10-12 | 邹城市洪石泉螺旋藻生物科技有限公司 | Preparation method of iron-rich instant spirulina |
| CN108315274A (en) * | 2017-12-25 | 2018-07-24 | 内蒙古乌审召生态产业发展有限公司 | A kind of spirulina breeding method and system |
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