CN108315274A - A kind of spirulina breeding method and system - Google Patents

A kind of spirulina breeding method and system Download PDF

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Publication number
CN108315274A
CN108315274A CN201711424390.5A CN201711424390A CN108315274A CN 108315274 A CN108315274 A CN 108315274A CN 201711424390 A CN201711424390 A CN 201711424390A CN 108315274 A CN108315274 A CN 108315274A
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spirulina
industrial wastewater
nutrient solution
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culture pond
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李京陆
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Inm Wushenzhao Ecologica Development Co Ltd
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12M23/02Form or structure of the vessel

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Abstract

The present invention provides a kind of spirulina breeding method and system.The method includes:Choose the industrial wastewater that thermal power plant generates during industrial water sofening treatment, preset nutriment is added into the industrial wastewater, prepare nutrient solution, the nutrient solution is thrown in spirulina culture pond, the spirulina in the spirulina culture pond is cultivated using the nutrient solution.Method provided in an embodiment of the present invention is using the industrial wastewater that thermal power plant generates during industrial water sofening treatment as water source, the underground water used in conventional helical algae cultural method is substituted using the waste water that industrial production is discharged, save the use of groundwater resources, reduce the aquaculture cost of spirulina, due to containing nutriment needed for a large amount of process of culturing spirulina in industrial wastewater, the additive amount of nutriment, further reduced aquaculture cost when therefore reducing nutrient solution.

Description

A kind of spirulina breeding method and system
Technical field
The present invention relates to spirulina breeding technology, more particularly to a kind of spirulina breeding method and a kind of spirulina are supported Grow system.
Background technology
Since nutritive component of spirulina is abundant, while there is preferable anti-virus ability, can effectively reduce blood glucose and courage The diseases such as sterol, so spirulina can be applied to the multiple fields such as food, health care.
At present in spirulina breeding technical field, compounding mainly is carried out using underground water and nutriment, is made Spirulina breeding is carried out with the nutrient solution.
There are the following problems for above-mentioned spirulina breeding method:It needs to expend when preparing nutrient solution used in cultivating spirulina A large amount of underground water causes the aquaculture cost of spirulina higher, while causing the waste of underground water;In addition, it is necessary to underground A large amount of nutriment is added in water, the addition of a large number of nutrients further increases the aquaculture cost of spirulina.
Invention content
Technical problem to be solved by the invention is to provide a kind of spirulina breeding methods overcoming the above problem.
To solve the above-mentioned problems, the invention discloses a kind of spirulina breeding method, the method includes:
Choose the industrial wastewater that thermal power plant generates during industrial water sofening treatment;
Preset nutriment is added into the industrial wastewater, prepares nutrient solution;
The nutrient solution is thrown in spirulina culture pond, using the nutrient solution in the spirulina culture pond Spirulina is cultivated.
Optionally, the industrial wastewater includes one or more objects in sodium bicarbonate, magnesium sulfate, potassium chloride and calcium chloride Matter.
Optionally, when the industrial wastewater includes sodium bicarbonate, magnesium sulfate, potassium chloride and calcium chloride, the Industry Waste In water, the concentration of calcium ion and magnesium ion and be 150-190mg/L;
The concentration of carbanion and nitrate ion and be 380-420mg/L;
A concentration of 80-100mg/L of chlorion;
The pH of the industrial wastewater is 8-9.
Optionally, the concentration of the calcium ion and the magnesium ion and be 165-175mg/L;
The concentration of the carbanion and the nitrate ion and be 390-410mg/L;
A concentration of 85-95mg/L of the chlorion;
The pH of the industrial wastewater is 8.3-8.7.
Optionally, the nutriment includes one or more in carbon source, nitrogen source, potassium resource and phosphorus source.
Optionally, the carbon source includes sodium bicarbonate;
The nitrogen source includes sodium nitrate;
The potassium resource includes potassium chloride;
Phosphorus source includes phosphoric acid.
Optionally, every liter of nutrient solution includes 2.5-5.0g sodium bicarbonates, 0.5-1.0g sodium nitrate, 0.5-1.0g chlorinations Potassium and 0.2-0.5g phosphoric acid;
The pH of the nutrient solution is 8.0-11.
Optionally, the OD value of the spirulina in the spirulina culture pond is 0.1-1.0Kg/m2
The invention also discloses a kind of spirulina breeding systems, make cultivating spirulina with the aforedescribed process, the spirulina Cultivating system includes spirulina culture pond, compounding device and industrial wastewater storage device, the industrial wastewater storage Device is connect with the compounding device, and the compounding device is connect with the spirulina culture pond;
The industrial wastewater storage device is used for the storage of industrial wastewater, and the industrial wastewater is thermal power plant in life It is generated during fisheries water sofening treatment;
The compounding device, is used for the preparation of nutrient solution, and the nutrient solution is stored according to the industrial wastewater What the industrial wastewater of device transmission was prepared;
The spirulina culture pond, is used for the cultivation of spirulina, and the spirulina is to use the compounding device The nutrient solution cultivation of transmission.
Optionally, the industrial wastewater storage device is industrial wastewater storage tank;
The compounding device is compounding pond.
Compared with prior art, the present invention includes following advantages:
An embodiment of the present invention provides a kind of spirulina breeding method and system.The method is using thermal power plant in life The industrial wastewater generated during fisheries water sofening treatment substitutes conventional helical as water source, using the waste water that industrial production is discharged Underground water used in algae cultural method saves the use of groundwater resources, reduces the aquaculture cost of spirulina, due to Nutrition when containing nutriment needed for a large amount of process of culturing spirulina in industrial wastewater, therefore reducing nutrient solution The additive amount of substance, further reduced aquaculture cost.
Meanwhile the industrial wastewater that is generated during industrial water sofening treatment to thermal power plant of this method has carried out time It receives and utilizes, eliminate the recovery processing step of the industrial wastewater discharged to industrial production in conventional method, eliminate to Industry Waste The subsequent treatment cost of water, protects Environmental security, improves the utility value of industrial wastewater.
Description of the drawings
Fig. 1 is a kind of method flow diagram of spirulina breeding method provided in an embodiment of the present invention.
Specific implementation mode
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below in conjunction with the accompanying drawings and specific real Applying mode, the present invention is described in further detail.
Spirulina breeding method of the present invention and spirulina breeding system are carried out below by embodiment detailed Explanation.
An embodiment of the present invention provides a kind of spirulina breeding methods.It illustrates provided in an embodiment of the present invention one by Fig. 1 The method flow diagram of kind spirulina breeding method, referring to Fig.1, the spirulina breeding method includes:
Step 101 chooses the industrial wastewater that thermal power plant generates during industrial water sofening treatment.
Thermal power plant uses underground water as industrial water, due to containing the objects such as a large amount of metal ion in underground water Matter, therefore before carrying out thermal power generation using underground water, need first to carry out sofening treatment to underground water, remove in underground water The substances such as metal ion.Membrane filtration or reverse osmosis process would generally be used to carry out sofening treatment to underground water, for underground water Sofening treatment technique, the embodiment of the present invention are not limited herein.
After carrying out sofening treatment to underground water, industrial wastewater is also obtained in underground water after being softened while, Include a large amount of salts substances in the industrial wastewater, industrial wastewater can be known as strong brine.
Based on the component difference and component ratio difference contained by underground water in different zones, thermal power plant is caused to produce With the component difference and component ratio difference contained by the industrial wastewater generated during water sofening treatment.For example, industrial wastewater can To include one or more substances in many kinds of substance, such as sodium bicarbonate, magnesium sulfate, potassium chloride and calcium chloride.
The above-mentioned substance for including due to the industrial wastewater that thermal power plant generates during industrial water sofening treatment Nutriment exactly needed for cultivating spirulina, therefore the embodiment of the present invention selects thermal power plant in industrial water sofening treatment The industrial wastewater generated in the process carries out the configuration of the nutrient solution needed for cultivating spirulina as water source, is cultivated using the nutrient solution Spirulina.
This method eliminates the use of underground water in traditional cultural method, saves groundwater resources, reduces spirulina Aquaculture cost.Compared to background technology the method cultivating spirulina, spiral is cultivated using the method for the embodiment of the present invention The aquaculture cost of spirulina can be reduced by 20% or more by algae.
In the prior art, at the recycling of the industrial wastewater generated during industrial water sofening treatment to thermal power plant Reason includes mainly following manner:The industrial chemicals such as coagulating agent are added into industrial wastewater, allow industrial chemicals in industrial wastewater Metal ion such as calcium ion, magnesium ion chemically reacts, and forms sediment, then handle by evaporation tank air dry, evaporates anti- The moisture in liquid is answered, then sediment is recycled, but this method processing step is cumbersome, takes longer, coagulating agent etc. The addition of industrial chemicals so that processing cost is higher;Alternatively, processing is filtered to industrial wastewater by mechanical equipment, then to mistake Reagent water after filter is recycled, but the infrastructure investment of this method is huge, and processing cost is very high.
The industrial wastewater that this method generates thermal power plant during industrial water sofening treatment is recycled, The recovery processing for eliminating industrial wastewater saves the cost recovery of industrial wastewater, protects Environmental security, improves Industry Waste The utility value of water.By taking installed capacity is the thermal power plant of 2 × 12MW scales as an example, the method for the embodiment of the present invention is used The industrial wastewater generated during industrial water sofening treatment to the factory is recycled, and can save industrial wastewater every year Processing cost is up to million yuan or more.
When specific implementation, in cultivating spirulina, a certain amount of thermal power plant is chosen in industrial water sofening treatment mistake The industrial wastewater generated in journey is used for subsequent configuration nutrient solution.
Step 102 adds preset nutriment into industrial wastewater, prepares nutrient solution.
It include a certain amount of spirulina in the industrial wastewater that thermal power plant generates during industrial water sofening treatment Nutriment needed for cultivation also needs to add other nutrition into industrial wastewater to use the industrial wastewater cultivating spirulina Substance, to configure the nutrient solution suitable for growth of spirulina platensis.
Since the component contained by underground water in different zones is different and component ratio is different, thermal power plant is in industrial water The component contained by industrial wastewater generated during sofening treatment is different and component ratio is different, it is therefore desirable to according to industrial wastewater Including nutriment type and ratio, the type and weight of nutriment for needing additionally to add are determined, so that configuration Nutrient solution be suitable for spirulina growth.
Illustratively, when industrial wastewater includes sodium bicarbonate, magnesium sulfate, potassium chloride and calcium chloride, in industrial wastewater, calcium from Son and magnesium ion concentration and can be 150-190mg/L;The concentration of carbanion and nitrate ion and can be 380- 420mg/L;The concentration of chlorion can be 80-100mg/L;The pH of industrial wastewater can be 8-9, under these conditions, spiral Algae can be with normal growth.Further, the concentration and preferably 165-175mg/L, carbanion and nitre of calcium ion and magnesium ion The concentration of the concentration of acid ion and preferably 390-410mg/L, chlorion are preferably 85-95mg/L, and the pH of industrial wastewater is excellent It is selected as 8.3-8.7, under above-mentioned optimum condition, spirulina can preferably be grown.
When industrial wastewater includes sodium bicarbonate, magnesium sulfate, potassium chloride and calcium chloride, the nutriment that additionally adds can be with Including one or more in carbon source, nitrogen source, potassium resource and phosphorus source.Specifically, carbon source may include sodium bicarbonate, and nitrogen source can wrap Sodium nitrate is included, potassium resource may include potassium chloride, and phosphorus source may include phosphoric acid.
To ensure spirulina normal growth in nutrient solution, in the nutrient solution configured by industrial wastewater and nutriment, often It includes 2.5-5.0g sodium bicarbonates, 0.5-1.0g sodium nitrate, 0.5-1.0g potassium chloride and 0.2-0.5g phosphoric acid, nutrition to rise nutrient solution The pH of liquid is 8.0-10.
Each Parameter Conditions that this example provides only are illustrated, and every Parameter Conditions suitable for the embodiment of the present invention are equal It can.
Nutrient solution is thrown in spirulina culture pond by step 103, using nutrient solution to the spiral in spirulina culture pond Algae is cultivated.
After having configured nutrient solution, nutrient solution is thrown in spirulina culture pond, using nutrient solution to spirulina breeding Spirulina in pond is cultivated.The injected volume of nutrient solution and the amount of spirulina to be cultivated and the size etc. of spirulina culture pond Relating to parameters, can be according to being actually configured.
The embodiment of the present invention use optical density detection method, to configured by above-mentioned example nutrient solution cultivation spirulina into Concentration Testing is gone, the OD value detected (Optical density, OD) is bigger, indicates that the content of spirulina is higher.Through It tests, the OD value of the spirulina in above-mentioned example in spirulina culture pond is 0.1-1.0Kg/m2.Test result shows The constituent species and component ratio of the industrial wastewater that above-mentioned example provides and the component of the nutriment provided in above-mentioned example Under type and component ratio, the nutrient solution configured is suitable for the growth of spirulina, i.e. spirulina can be configured in above-mentioned example Normal growth in nutrient solution.
After the completion of spirulina breeding, the processing such as it can be filtered, clean, dry in processing workshop, it is dry to obtain spirulina Powder.Can forming processes be carried out to spirulina powder according to actual needs, such as carry out compressing tablet process, obtain the spiral shell of required structure Revolve algae product.
To make those skilled in the art that the present invention be more clearly understood, following example is now enumerated to the embodiment of the present invention Spirulina breeding method is described in detail.
Example 1
The industrial wastewater that thermal power plant generates during industrial water sofening treatment is chosen, industrial wastewater includes carbonic acid Hydrogen sodium, magnesium sulfate, potassium chloride and calcium chloride, wherein the concentration of calcium ion and magnesium ion and be 165-175mg/L;Carbonate from Son and nitrate ion concentration and can be 390-410mg/L;The concentration of chlorion can be 85-95mg/L;Industrial wastewater PH be 8.3-8.7.
Sodium bicarbonate, sodium nitrate, potassium chloride and phosphoric acid are added into industrial wastewater, prepare nutrient solution.The nutrient solution of preparation In, every liter of nutrient solution includes 3.5-5.0g sodium bicarbonates, 0.8-1.0g sodium nitrate, 0.8-1.0g potassium chloride and 0.3-0.5g phosphorus The pH of acid, nutrient solution is 9.0-11.
The nutrient solution of configuration is launched into spirulina culture pond, using the nutrient solution to the spiral in spirulina culture pond Algae is cultivated.
Example 2
The industrial wastewater that thermal power plant generates during industrial water sofening treatment is chosen, industrial wastewater includes carbonic acid Hydrogen sodium, magnesium sulfate, potassium chloride and calcium chloride, wherein the concentration of calcium ion and magnesium ion and be 175-190mg/L;Carbonate from Son and nitrate ion concentration and can be 400-420mg/L;The concentration of chlorion can be 90-100mg/L;Industrial wastewater PH be 8.5-9.
Sodium bicarbonate, sodium nitrate, potassium chloride and phosphoric acid are added into industrial wastewater, prepare nutrient solution.The nutrient solution of preparation In, every liter of nutrient solution includes 4.0-5.0g sodium bicarbonates, 0.5-0.7g sodium nitrate, 0.5-0.7g potassium chloride and 0.2-0.4g phosphorus The pH of acid, nutrient solution is 8.0-11.
The nutrient solution of configuration is launched into spirulina culture pond, using the nutrient solution to the spiral in spirulina culture pond Algae is cultivated.
Example 3
The industrial wastewater that thermal power plant generates during industrial water sofening treatment is chosen, industrial wastewater includes carbonic acid Hydrogen sodium, magnesium sulfate, potassium chloride and calcium chloride, wherein the concentration of calcium ion and magnesium ion and be 150-170mg/L;Carbonate from Son and nitrate ion concentration and can be 380-400mg/L;The concentration of chlorion can be 80-90mg/L;Industrial wastewater PH be 8-8.5.
Sodium bicarbonate, sodium nitrate, potassium chloride and phosphoric acid are added into industrial wastewater, prepare nutrient solution.The nutrient solution of preparation In, every liter of nutrient solution includes 2.5-3.5g sodium bicarbonates, 08-1.0g sodium nitrate, 0.8-1.0g potassium chloride and 0.3-0.5g phosphoric acid, The pH of nutrient solution is 8-9.
The nutrient solution of configuration is launched into spirulina culture pond, using the nutrient solution to the spiral in spirulina culture pond Algae is cultivated.
The embodiment of the present invention additionally provides a kind of spirulina breeding system, which uses the above-mentioned offer of the embodiment of the present invention Method cultivating spirulina.
Spirulina breeding system provided in an embodiment of the present invention includes spirulina culture pond, compounding device and gives up Water storage apparatus, wherein industrial wastewater storage device is connect with compounding device, and compounding device is supported with spirulina Grow pond connection;
Effluent storage device is used for the storage of industrial wastewater, and industrial wastewater is thermal power plant at industrial water softening What reason generated in the process;
Compounding device is used for the preparation of nutrient solution, and nutrient solution is the Industry Waste transmitted according to effluent storage device What water was prepared;
Spirulina culture pond, is used for the cultivation of spirulina, and spirulina is the nutrient solution transmitted using compounding device Cultivation.
Connection type between device can there are many, for example, can between effluent storage device and compounding device To be connected by pipeline, control valve can be provided on pipeline;It can be between compounding device and spirulina culture pond It is connected by pipeline, control valve can be provided on pipeline.Connection type between device can be according to being actually configured.
It, can be according to actual setting device or the concrete structure of equipment based on the function of each device or equipment in system.Example Such as, effluent storage device can be effluent storage tank;Compounding device can be compounding pond.
Since spirulina breeding system provided in an embodiment of the present invention cultivates spiral shell using method provided in an embodiment of the present invention Algae is revolved, uses the industrial wastewater generated during industrial water sofening treatment to thermal power plant in breeding process, therefore can In thermal power plant peripheral region, can specifically build spirulina breeding system Construction in thermal power plant peripheral region And meet the region for meeting food production processing conditions, it is generated during industrial water sofening treatment convenient for thermal power plant The conveying and use of industrial wastewater.
A kind of spirulina breeding method provided by the present invention and a kind of spirulina breeding system have been carried out in detail above Thin to introduce, principle and implementation of the present invention are described for specific case used herein, and above example is said The bright method and its core concept for being merely used to help understand the present invention;Meanwhile for those of ordinary skill in the art, foundation The thought of the present invention, there will be changes in the specific implementation manner and application range, in conclusion the content of the present specification is not It is interpreted as limitation of the present invention.

Claims (10)

1. a kind of spirulina breeding method, which is characterized in that the method includes:
Choose the industrial wastewater that thermal power plant generates during industrial water sofening treatment;
Preset nutriment is added into the industrial wastewater, prepares nutrient solution;
The nutrient solution is thrown in spirulina culture pond, using the nutrient solution to the spiral in the spirulina culture pond Algae is cultivated.
2. according to the method described in claim 1, it is characterized in that, the industrial wastewater includes sodium bicarbonate, magnesium sulfate, chlorination One or more substances in potassium and calcium chloride.
3. according to the method described in claim 2, it is characterized in that, when the industrial wastewater includes sodium bicarbonate, magnesium sulfate, chlorine Change potassium and when calcium chloride, in the industrial wastewater, the concentration of calcium ion and magnesium ion and be 150-190mg/L;
The concentration of carbanion and nitrate ion and be 380-420mg/L;
A concentration of 80-100mg/L of chlorion;
The pH of the industrial wastewater is 8-9.
4. according to the method described in claim 3, it is characterized in that,
The concentration of the calcium ion and the magnesium ion and be 165-175mg/L;
The concentration of the carbanion and the nitrate ion and be 390-410mg/L;
A concentration of 85-95mg/L of the chlorion;
The pH of the industrial wastewater is 8.3-8.7.
5. according to the method described in claim 3, it is characterized in that, the nutriment includes carbon source, nitrogen source, potassium resource and phosphorus source In it is one or more.
6. according to the method described in claim 5, it is characterized in that, the carbon source includes sodium bicarbonate;
The nitrogen source includes sodium nitrate;
The potassium resource includes potassium chloride;
Phosphorus source includes phosphoric acid.
7. according to the method described in claim 6, it is characterized in that, every liter of nutrient solution include 2.5-5.0g sodium bicarbonates, 0.5-1.0g sodium nitrate, 0.5-1.0g potassium chloride and 0.2-0.5g phosphoric acid;
The pH of the nutrient solution is 8.0-11.
8. the method according to the description of claim 7 is characterized in that the OD value of the spirulina in the spirulina culture pond For 0.1-1.0Kg/m2
9. a kind of spirulina breeding system, which is characterized in that cultivate spiral using claim 1-8 any one of them methods Algae, the spirulina breeding system include spirulina culture pond, compounding device and effluent storage device, the industry Effluent storage device is connect with the compounding device, and the compounding device connects with the spirulina culture pond It connects;
The effluent storage device is used for the storage of industrial wastewater, and the industrial wastewater is that thermal power plant is soft in industrial water It is generated in change processing procedure;
The compounding device, is used for the preparation of nutrient solution, and the nutrient solution is transmitted according to the effluent storage device Industrial wastewater prepare;
The spirulina culture pond, is used for the cultivation of spirulina, and the spirulina is transmitted using the compounding device Nutrient solution cultivation.
10. according to the method described in claim 9, it is characterized in that, the effluent storage device is effluent storage tank;
The compounding device is compounding pond.
CN201711424390.5A 2017-12-25 2017-12-25 A kind of spirulina breeding method and system Pending CN108315274A (en)

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Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000055104A1 (en) * 1999-03-18 2000-09-21 Oms Investments, Inc. Processes for preparing granular composite fertilizer compositions and products produced thereby
CN104017762A (en) * 2014-06-25 2014-09-03 京环央都南通生物医药研究院有限公司 Culture method of spirulina

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Application publication date: 20180724