CN107827244A - Bacterial-algae complexing agent for aquaculture sewage treatment and preparation method thereof - Google Patents
Bacterial-algae complexing agent for aquaculture sewage treatment and preparation method thereof Download PDFInfo
- Publication number
- CN107827244A CN107827244A CN201711429007.5A CN201711429007A CN107827244A CN 107827244 A CN107827244 A CN 107827244A CN 201711429007 A CN201711429007 A CN 201711429007A CN 107827244 A CN107827244 A CN 107827244A
- Authority
- CN
- China
- Prior art keywords
- parts
- complexing agent
- algae
- aquaculture wastewater
- wastewater processing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Cell Biology (AREA)
- Ecology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a bacteria-algae complexing agent for aquaculture sewage treatment and a preparation method thereof, wherein the bacteria-algae complexing agent for aquaculture sewage treatment comprises the following raw materials in parts by weight: 15-18 parts of microalgae harvest, 12-14 parts of bacillus licheniformis culture solution, 9-11 parts of candida utilis culture solution and 4-6 parts of soybean straw powder; the preparation method comprises the following steps: uniformly mixing the bacillus licheniformis culture solution and the candida utilis culture solution to obtain a mixture A; uniformly mixing the soybean straw powder and the microalgae harvest to obtain a mixture B; and adding the mixture B into the mixture A, and stirring for 30-40 minutes to obtain the bacteria-algae complexing agent for treating the culture sewage. The bacteria-algae complexing agent for treating the culture sewage can construct a bacteria-algae symbiotic system in the culture sewage, effectively remove nitrogen and phosphorus pollutants in the culture sewage and reduce the COD value.
Description
Technical field
The present invention relates to aquaculture wastewater process field, more particularly to a kind of bacterium algae complexing agent for aquaculture wastewater processing and
Its preparation method.
Background technology
With the large-scale development of China's livestock and poultry breeding industry, per annual meeting, the livestock and poultry cultivation excrement of nearly 1,000,000,000 tons of generation is dirty, to having
The water resource of limit causes serious threat.
At present, the processing mode of aquaculture wastewater mainly has two kinds：First, being handled using methane-generating pit anaerobic fermentation, but produce
The nutritive salt such as the nitrogen phosphorus in the biogas slurry of raw enormous amount still containing high concentration, with the propulsion of the Course of Urbanization, disappear
Receive biogas slurry arable land it is increasingly insufficient, caused biogas slurry is directly discharged in water body, it will causes natural water serious eutrophication；
Second, bioanalysis removes, biological treatment is the pollutant in sewage to be degraded using microorganisms such as bacterium, fungies and/or together
Change and absorb, realize the purification of sewage, but due to aquaculture wastewater by Anaerobic Treatment it is latter as available carbon source it is too low, can not
Meet the needs of heterotrophism function bacterium, therefore to nitrate and phosphatic poor processing effect, it is necessary to physical filtering and chemisorbed
It is used in combination Deng various ways, this not only adds processing cost, also increases potential secondary pollution risk.
Microalgae facture is sewage treatmentmethod emerging at present, the abundant nitrogen contained in livestock and poultry cultivation sewage, phosphorus etc.
Nutriment can provide nutrition for microalgae, and handling sewage using microalgae can join with the large-scale production phase lotus root of microalgae simultaneously, realize
Resource recycles.
With microalgae purifying water effect and algae and microorganism, repercussion study is goed deep into water body, helotism system
Application on livestock and poultry cultivation sewage has obtained common concern.Helotism system be by algae to nitrogen and phosphorus thing in sewage and
The intake of organic matter removes a kind of mutualism system that effect contaminant degradation ability powerful with microorganism effectively combines
System.Therefore, the present invention proposes a kind of bacterium algae complexing agent for aquaculture wastewater processing, it is dirty that the bacterium algae complexing agent is put into cultivation
In water, you can build the helotism system for handling aquaculture wastewater.
The content of the invention
Based on technical problem existing for background technology, it is compound that the present invention proposes a kind of bacterium algae for aquaculture wastewater processing
Agent and preparation method thereof, bacterium can be built in aquaculture wastewater provided by the present invention for the bacterium algae complexing agent of aquaculture wastewater processing
Algae cogeneration system, the nitrogen and phosphorus pollution thing in aquaculture wastewater is effectively removed, reduces COD value, bacterium algae complexing agent provided by the invention
Preparation method is simple to operate, and energy consumption is low, is adapted to commercial Application.
The invention provides a kind of bacterium algae complexing agent for aquaculture wastewater processing, its raw material includes by weight：Microalgae
Harvested material 15-18 parts, bacillus licheniformis nutrient solution 12-14 parts, candida utili nutrient solution 9-11 parts, soybean stalk powder 4-
6 parts.
Preferably, its raw material includes by weight：16.3 parts of microalgae recovery thing, 12.8 parts of bacillus licheniformis nutrient solution,
10.2 parts of candida utili nutrient solution, 4.7 parts of soybean stalk powder.
Preferably, the preparation method of microalgae recovery thing comprises the following steps：
S1, under aseptic technique, by chlorella vulgaris with 1 × 106Individual/mL-1 × 107Individual/mL inoculum concentration is inoculated into training
Support in base, be 25-27 DEG C in temperature, under the conditions of rotating speed is 110-130 revs/min, light dark cycle alternate culture 60-75 is small
When, obtain micro algae culturing liquid；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.1-5.4, adds the 1.5%- of micro algae culturing liquid weight
1.7% sodium citrate, 20-30 minutes are stirred, add the 3.8%-4.3% of micro algae culturing liquid weight calcium chloride, stir 40-50
Minute, centrifuged with 2000-2200 revs/min of revolution, obtain microalgae recovery thing.
Preferably, the raw material of culture medium includes according to parts by weight in the preparation method S1 of microalgae recovery thing：1-2 part grapes
Sugar, 4-6 parts starch sugar, 0.01-0.03 parts zinc sulfate, 0.1-0.3 parts magnesium sulfate, 0.15-0.3 parts ammonium sulfate, 0.2-0.4 part phosphorus
Acid dihydride potassium, 0.1-0.3 parts sodium nitrate, 9-12 parts sodium chloride and 240-260 part deionized waters.
Preferably, in the preparation method S1 light dark cycle alternate cultures of microalgae recovery thing, cultivated under photoenvironment
Time be 8-10 hours, the time cultivated under dark surrounds is 11-13 hours, photoenvironment, dark surrounds cycle alternation
Carry out.
Preferably, in the preparation method S1 light dark cycle alternate cultures of microalgae recovery thing, the illumination of photoenvironment is strong
Spend for 3500-3800Lux.
Preferably, bacillus licheniformis nutrient solution is prepared with the following method：By bacillus licheniformis with 5 × 107Individual/
mL-8×107Individual/mL inoculum concentration is inoculated into the D/W that mass fraction is 1%-3%, and 40- is cultivated at 25-35 DEG C
60 minutes, obtain bacillus licheniformis nutrient solution.
Preferably, candida utili bacteria culture fluid is prepared with the following method：By candida utili bacterium with 1 × 106
Individual/mL-3 × 106Individual/mL inoculum concentration is inoculated into the D/W that mass fraction is 0.3%-0.7%, at 30-40 DEG C
20-30 minutes are cultivated, obtain candida utili bacteria culture fluid.
It is used for the preparation method of the bacterium algae complexing agent of aquaculture wastewater processing the invention provides this kind, comprises the following steps：
Step 1: bacillus licheniformis nutrient solution and candida utili bacteria culture fluid are well mixed, mixture A is obtained；
Step 2: soybean stalk powder is well mixed with microalgae recovery thing, mixture B is obtained；
Step 3: the mixture B obtained in step 2 is added in the mixture A obtained in step 1,30-40 minutes are stirred
Obtain the bacterium algae complexing agent for aquaculture wastewater processing.
Helotism can be built in aquaculture wastewater provided by the present invention for the bacterium algae complexing agent of aquaculture wastewater processing
System, the helotism system are aided with microorganism to organic matter degradation, are effectively removed cultivation based on the assimilation of algae
Nitrogen and phosphorus pollution thing in sewage, COD value is reduced, adapts to, in various complicated growing environments, have broad application prospects.
The preparation method of bacterium algae complexing agent provided by the invention is simple to operate, and energy consumption is low, is adapted to commercial Application.
Microalgae in bacterium algae complexing agent provided by the invention using its in growth course to organic matter in aquaculture wastewater,
The absorption of nutrient ingredients such as nitrogen, phosphorus, own cells skeleton is synthesized to purify livestock and poultry cultivation sewage, and can remove removing heavy metals and difficulty
Degradation of organic substances.
The preparation method of microalgae recovery thing mainly includes two parts of culture and harvesting of chlorella vulgaris in the present invention.It is general
The culture of logical chlorella uses light dark cycle alternate culture method, and preferred photoenvironment, dark surrounds incubation time,
After light dark cycle alternate culture 60-75 hours, terminate culture.Invention technician is had found when cultivating 60-75 hours
Between terminate chlorella vulgaris that culture obtains in section after livestock and poultry cultivation sewage is entered, its speed grown is better than in other times
Terminate the chlorella vulgaris that culture obtains in section.The harvesting of chlorella vulgaris adds lemon by adjusting micro algae culturing liquid pH value
Sour sodium, the mode of calcium chloride make chlorella flocculate and then separated from liquid, and this method is simple to operate, and energy consumption is low, are adapted to
Commercial Application.Have after sewage is put into that micro algae growth is rapid, nitrogen phosphorus uptake is big using the chlorella vulgaris that this method obtains,
The advantages that pollutant elimination efficiency is high.
Embodiment
Below, technical scheme is described in detail by specific embodiment.
Embodiment 1
The invention provides a kind of bacterium algae complexing agent for aquaculture wastewater processing, its raw material includes by weight：Microalgae recovery
16.3 parts of thing, 12.8 parts of bacillus licheniformis nutrient solution, 10.1 parts of candida utili nutrient solution, 4.7 parts of soybean stalk powder.
Microalgae recovery thing is prepared using following methods：
S1, under aseptic technique, by chlorella vulgaris（Chlorella vulgaris）With 5.4 × 106Individual/mL inoculation
Amount is inoculated into culture medium, is 26 DEG C in temperature, under the conditions of rotating speed is 122 revs/min, is carried out light dark cycle and is alternately trained
Support, cultivated 9 hours, intensity of illumination 3700Lux under photoenvironment, the time cultivated under dark surrounds is 11 hours, light
After environment, dark surrounds cycle alternation culture 68 hours, micro algae culturing liquid is obtained；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.2, adds 1.58% lemon of micro algae culturing liquid weight
Sour sodium, stir 25 minutes, add 4.1% calcium chloride of micro algae culturing liquid weight, stir 44 minutes, with 2100 revs/min turn
Number centrifuges, and obtains microalgae recovery thing.
Wherein, the raw material of culture medium includes according to parts by weight in S1：1.6 parts of glucose, 5.2 portions of starch sugars, 0.02 part of sulphur
Sour zinc, 0.19 part of magnesium sulfate, 0.23 part of ammonium sulfate, 0.28 part of potassium dihydrogen phosphate, 0.21 part of sodium nitrate, 11 parts of sodium chloride and 250
Part deionized water.
Bacillus licheniformis nutrient solution is prepared with the following method：By bacillus licheniformis with 6.2 × 107Individual/mL's connects
Kind amount is inoculated into the D/W that mass fraction is 1.7%, is cultivated 53 minutes at 27 DEG C, obtains bacillus licheniformis training
Nutrient solution.
Candida utili bacteria culture fluid is prepared with the following method：By candida utili bacterium with 2.1 × 106Individual/mL
Inoculum concentration be inoculated into mass fraction be 0.56% D/W in, 37 DEG C cultivate 24 minutes, obtain Candida utilis ferment
Female bacteria culture fluid.
Embodiment 2
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, its raw material can also include by weight：Microalgae recovery thing
17.6 parts, 13.3 parts of bacillus licheniformis nutrient solution, 9.2 parts of candida utili nutrient solution, 5.8 parts of soybean stalk powder.
Embodiment 3
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, its raw material can also include by weight：Microalgae recovery thing
15.2 parts, 13.8 parts of bacillus licheniformis nutrient solution, 10.7 parts of candida utili nutrient solution, 4.3 parts of soybean stalk powder.
Embodiment 4
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, its raw material can also include by weight：Microalgae recovery thing
16.9 parts, 12.2 parts of bacillus licheniformis nutrient solution, 10.4 parts of candida utili nutrient solution, 5.1 parts of soybean stalk powder.
Embodiment 5
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, microalgae recovery thing can also use following methods to prepare：
S1, under aseptic technique, by chlorella vulgaris with 1.6 × 106Individual/mL inoculum concentration is inoculated into culture medium,
Temperature is 25 DEG C, under the conditions of rotating speed is 128 revs/min, carries out light dark cycle alternate culture, 10 are cultivated under photoenvironment
Hour, intensity of illumination 3600Lux, the time cultivated under dark surrounds is 12 hours, and photoenvironment, dark surrounds circulation are handed over
After culture 73 hours, micro algae culturing liquid is obtained；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.3, adds 1.67% lemon of micro algae culturing liquid weight
Sour sodium, stir 22 minutes, add 3.9% calcium chloride of micro algae culturing liquid weight, stir 48 minutes, with 2200 revs/min turn
Number centrifuges, and obtains microalgae recovery thing.
Wherein, the raw material of culture medium includes according to parts by weight in S1：1.9 parts of glucose, 4.3 portions of starch sugars, 0.01 part of sulphur
Sour zinc, 0.12 part of magnesium sulfate, 0.17 part of ammonium sulfate, 0.38 part of potassium dihydrogen phosphate, 0.27 part of sodium nitrate, 10 parts of sodium chloride and 256
Part deionized water.
Embodiment 6
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, microalgae recovery thing can also use following methods to prepare：
S1, under aseptic technique, by chlorella vulgaris with 8.9 × 106Individual/mL inoculum concentration is inoculated into culture medium,
Temperature is 27 DEG C, under the conditions of rotating speed is 113 revs/min, carries out light dark cycle alternate culture, 8 are cultivated under photoenvironment
Hour, intensity of illumination 3800Lux, the time cultivated under dark surrounds is 13 hours, and photoenvironment, dark surrounds circulation are handed over
After culture 62 hours, micro algae culturing liquid is obtained；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.1, adds 1.51% lemon of micro algae culturing liquid weight
Sour sodium, stir 28 minutes, add 4.2% calcium chloride of micro algae culturing liquid weight, stir 41 minutes, with 2000 revs/min turn
Number centrifuges, and obtains microalgae recovery thing.
Wherein, the raw material of culture medium includes according to parts by weight in S1：1.2 parts of glucose, 5.8 portions of starch sugars, 0.03 part of sulphur
Sour zinc, 0.27 part of magnesium sulfate, 0.28 part of ammonium sulfate, 0.23 part of potassium dihydrogen phosphate, 0.14 part of sodium nitrate, 9 parts of sodium chloride and 241 parts
Deionized water.
Embodiment 7
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, bacillus licheniformis nutrient solution can also use such as lower section
It is prepared by method：By bacillus licheniformis with 7.7 × 107It is water-soluble that individual/mL inoculum concentration is inoculated into the glucose that mass fraction is 1.1%
In liquid, cultivated 42 minutes at 33 DEG C, obtain bacillus licheniformis nutrient solution.
Embodiment 8
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, bacillus licheniformis nutrient solution can also use such as lower section
It is prepared by method：By bacillus licheniformis with 5.4 × 107It is water-soluble that individual/mL inoculum concentration is inoculated into the glucose that mass fraction is 2.6%
In liquid, cultivated 58 minutes at 31 DEG C, obtain bacillus licheniformis nutrient solution.
Embodiment 9
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, candida utili bacteria culture fluid can also use as follows
It is prepared by method：By candida utili bacterium with 1.3 × 106Individual/mL inoculum concentration is inoculated into the glucose that mass fraction is 0.65%
In the aqueous solution, cultivated 28 minutes at 31 DEG C, obtain candida utili bacteria culture fluid.
Embodiment 10
The bacterium algae complexing agent for being used for aquaculture wastewater processing of embodiment 1, candida utili bacteria culture fluid can also use as follows
It is prepared by method：By candida utili bacterium with 2.7 × 106Individual/mL inoculum concentration is inoculated into the glucose that mass fraction is 0.36%
In the aqueous solution, cultivated 22 minutes at 34 DEG C, obtain candida utili bacteria culture fluid.
Embodiment 11
The invention provides a kind of preparation method of the bacterium algae complexing agent for aquaculture wastewater processing, comprise the following steps：
Step 1: 12.8 parts of bacillus licheniformis nutrient solutions and 10.1 parts of candida utili bacteria culture fluids are well mixed, obtain
To mixture A；
Step 2: 4.7 parts of soybean stalk powder are well mixed with 16.3 parts of microalgae recovery things, mixture B is obtained；
Step 3: the mixture B obtained in step 2 is added in the mixture A obtained in step 1, stirring obtains for 33 minutes
To the bacterium algae complexing agent handled for aquaculture wastewater.
Embodiment 12
The invention provides a kind of preparation method of the bacterium algae complexing agent for aquaculture wastewater processing, comprise the following steps：
Step 1: 13.3 parts of bacillus licheniformis nutrient solutions and 9.2 parts of candida utili bacteria culture fluids are well mixed, obtain
Mixture A；
Step 2: 5.8 parts of soybean stalk powder are well mixed with 17.6 parts of microalgae recovery things, mixture B is obtained；
Step 3: the mixture B obtained in step 2 is added in the mixture A obtained in step 1, stirring obtains for 37 minutes
To the bacterium algae complexing agent handled for aquaculture wastewater.
The chlorella vulgaris being inoculated into the present invention in the preparation method S1 of microalgae recovery thing in culture medium is preferably
Activation method well known by persons skilled in the art, preferably slant activation method can be selected in the chlorella vulgaris activated, activation method；
Micro algae culturing liquid pH regulation can preferably add the hydrochloric acid that mass fraction is 30%-40% by the way of hydrochloric acid is added in S2.
The methods of inoculation that is related in the present invention, stirring, centrifugation, configuration of culture medium be this area it is known often
Know, will not be repeated here.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (9)
1. a kind of bacterium algae complexing agent for aquaculture wastewater processing, it is characterised in that its raw material includes by weight：Microalgae recovery
Thing 15-18 parts, bacillus licheniformis nutrient solution 12-14 parts, candida utili nutrient solution 9-11 parts, soybean stalk powder 4-6 parts.
2. a kind of bacterium algae complexing agent for aquaculture wastewater processing according to claim 1, it is characterised in that its raw material is pressed
Parts by weight include：16.3 parts of microalgae recovery thing, 12.8 parts of bacillus licheniformis nutrient solution, candida utili nutrient solution 10.1
Part, 4.7 parts of soybean stalk powder.
A kind of 3. bacterium algae complexing agent for aquaculture wastewater processing according to claim 1, it is characterised in that the microalgae
The preparation method of harvested material comprises the following steps：
S1, under aseptic technique, by chlorella vulgaris with 1 × 106Individual/mL-1 × 107Individual/mL inoculum concentration is inoculated into training
Support in base, be 25-27 DEG C in temperature, under the conditions of rotating speed is 110-130 revs/min, light dark cycle alternate culture 60-75 is small
When, obtain micro algae culturing liquid；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.1-5.4, adds the 1.5%- of micro algae culturing liquid weight
1.7% sodium citrate, 20-30 minutes are stirred, add the 3.8%-4.3% of micro algae culturing liquid weight calcium chloride, stir 40-50
Minute, centrifuged with 2000-2200 revs/min of revolution, obtain microalgae recovery thing.
A kind of 4. bacterium algae complexing agent for aquaculture wastewater processing according to claim 3, it is characterised in that the microalgae
The raw material of culture medium includes according to parts by weight in the preparation method S1 of harvested material：1-2 parts glucose, 4-6 parts starch sugar, 0.01-
0.03 part of zinc sulfate, 0.1-0.3 parts magnesium sulfate, 0.15-0.3 parts ammonium sulfate, 0.2-0.4 parts potassium dihydrogen phosphate, 0.1-0.3 part nitre
Sour sodium, 9-12 parts sodium chloride and 240-260 part deionized waters.
A kind of 5. bacterium algae complexing agent for aquaculture wastewater processing according to claim 3, it is characterised in that the microalgae
In the preparation method S1 light dark cycle alternate cultures of harvested material, the time cultivated under photoenvironment is 8-10 hours,
The time cultivated under dark surrounds is 11-13 hours, and photoenvironment, dark surrounds cycle alternation are carried out.
A kind of 6. bacterium algae complexing agent for aquaculture wastewater processing according to claim 5, it is characterised in that the microalgae
In the preparation method S1 light dark cycle alternate cultures of harvested material, the intensity of illumination of photoenvironment is 3500-3800Lux.
A kind of 7. bacterium algae complexing agent for aquaculture wastewater processing according to claim 1, it is characterised in that the lichens
Bacillus nutrient solution is prepared with the following method：By bacillus licheniformis with 5 × 107Individual/mL-8 × 107Individual/mL inoculum concentration
It is inoculated into the D/W that mass fraction is 1%-3%, cultivates 40-60 minutes at 25-35 DEG C, obtain bacillus licheniformis
Nutrient solution.
A kind of 8. bacterium algae complexing agent for aquaculture wastewater processing according to claim 1, it is characterised in that the production protein
Candida bacteria culture fluid is prepared with the following method：By candida utili bacterium with 1 × 106Individual/mL-3 × 106Individual/mL's connects
Kind amount is inoculated into the D/W that mass fraction is 0.3%-0.7%, and 20-30 minutes are cultivated at 30-40 DEG C, obtains producing protein
Candida bacteria culture fluid.
9. the preparation method of a kind of bacterium algae complexing agent for aquaculture wastewater processing, it is characterised in that comprise the following steps：
Step 1: bacillus licheniformis nutrient solution and candida utili bacteria culture fluid are well mixed, mixture A is obtained；
Step 2: soybean stalk powder is well mixed with microalgae recovery thing, mixture B is obtained；
Step 3: the mixture B obtained in step 2 is added in the mixture A obtained in step 1,30-40 minutes are stirred
Obtain the bacterium algae complexing agent for aquaculture wastewater processing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711429007.5A CN107827244A (en) | 2017-12-26 | 2017-12-26 | Bacterial-algae complexing agent for aquaculture sewage treatment and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711429007.5A CN107827244A (en) | 2017-12-26 | 2017-12-26 | Bacterial-algae complexing agent for aquaculture sewage treatment and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107827244A true CN107827244A (en) | 2018-03-23 |
Family
ID=61645473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711429007.5A Pending CN107827244A (en) | 2017-12-26 | 2017-12-26 | Bacterial-algae complexing agent for aquaculture sewage treatment and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107827244A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112047492A (en) * | 2020-09-04 | 2020-12-08 | 镇江立嘉农业发展有限公司 | Fish culture sewage treatment preparation and application method thereof |
| CN112624250A (en) * | 2020-12-18 | 2021-04-09 | 天津现代晨辉科技集团有限公司 | Water quality regulator for aquaculture |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402934A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Water quality purification bacterium agent from mix fermentation production with multiple bacterials and production method thereof |
| CN104710003A (en) * | 2015-02-12 | 2015-06-17 | 宁波市海洋与渔业研究院 | Purifying agent for improving shellfish cultivation environment |
| CN205347412U (en) * | 2016-02-16 | 2016-06-29 | 金华职业技术学院 | Little algae culture apparatus that gathers |
| CN107129950A (en) * | 2017-06-09 | 2017-09-05 | 复旦大学 | A kind of active phycomycete community and preparation method for purifying domestic sewage |
| CN107213674A (en) * | 2017-06-28 | 2017-09-29 | 常州市万昌化工有限公司 | A kind of method that utilization microalgae prepares microalgae flocculant |
-
2017
- 2017-12-26 CN CN201711429007.5A patent/CN107827244A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402934A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Water quality purification bacterium agent from mix fermentation production with multiple bacterials and production method thereof |
| CN104710003A (en) * | 2015-02-12 | 2015-06-17 | 宁波市海洋与渔业研究院 | Purifying agent for improving shellfish cultivation environment |
| CN205347412U (en) * | 2016-02-16 | 2016-06-29 | 金华职业技术学院 | Little algae culture apparatus that gathers |
| CN107129950A (en) * | 2017-06-09 | 2017-09-05 | 复旦大学 | A kind of active phycomycete community and preparation method for purifying domestic sewage |
| CN107213674A (en) * | 2017-06-28 | 2017-09-29 | 常州市万昌化工有限公司 | A kind of method that utilization microalgae prepares microalgae flocculant |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112047492A (en) * | 2020-09-04 | 2020-12-08 | 镇江立嘉农业发展有限公司 | Fish culture sewage treatment preparation and application method thereof |
| CN112624250A (en) * | 2020-12-18 | 2021-04-09 | 天津现代晨辉科技集团有限公司 | Water quality regulator for aquaculture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107937382B (en) | Preparation method of immobilized microalgae | |
| CN102584430B (en) | Method for manufacturing amino-acid fishpond fertilizer water aqua by waste bave protein | |
| CN102718325B (en) | Method for culturing high-density oil microalgae to treat yeast industrial wastewater | |
| CN107287125B (en) | Method for culturing chlorella pyrenoidosa | |
| CN1807588A (en) | Composite bacillus subtilis and lactic acid bacteria microbe formulation preparation method | |
| CN105152466A (en) | Method for treating waste water of aquatic bird cultivation through utilizing microalgae | |
| CN104593262A (en) | Series cultivation and rapid collection method for marine microalgae | |
| CN108033574A (en) | A kind of Ecological water purification agent for city river purification and preparation method thereof | |
| CN108220165A (en) | A kind of method for strengthening microalgae removal breeding wastewater carbon nitrogen and phosphorus pollution using association flora | |
| CN107974411A (en) | A kind of preparation method of Immobilized hyphae microballoon | |
| CN108017155A (en) | A kind of preparation method of microalgae sewage-treating agent | |
| CN107265612A (en) | Aerobic biochemical water purification agent | |
| JP2014533499A (en) | Cleaning liquid | |
| CN107827244A (en) | Bacterial-algae complexing agent for aquaculture sewage treatment and preparation method thereof | |
| CN102511683A (en) | Alga nutrient solution for prawn culture and preparation method thereof | |
| CN105002110B (en) | Complex microorganism preparations and its application in the processing of algal bloom water body | |
| CN106635919A (en) | Spirulina culture method | |
| CN1289659C (en) | High temperature neutral protenase strains, high temperature neutral proleinase and process thereof | |
| CN107841464A (en) | A kind of cultural method of algae | |
| CN101824385A (en) | Method for cultivating chlorella with methane waste liquor | |
| CN101153297A (en) | Novel single-tank hemicontinuous high-strength ferment high optical purity L- lactic acid technique for rhizopus oryzae bacterium ball | |
| CN101153294A (en) | Immobilized cell single-tank high-strength continuous fermentation process for succinic acid | |
| CN110484476A (en) | A kind of cultural method of spirulina | |
| CN105400697A (en) | Method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in carbon dioxide environment | |
| CN107814440A (en) | Bacterial-algae complexing agent for sewage treatment and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180323 |
|
| RJ01 | Rejection of invention patent application after publication |