CN107814440A - Bacterial-algae complexing agent for sewage treatment and preparation method thereof - Google Patents
Bacterial-algae complexing agent for sewage treatment and preparation method thereof Download PDFInfo
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- CN107814440A CN107814440A CN201711429461.0A CN201711429461A CN107814440A CN 107814440 A CN107814440 A CN 107814440A CN 201711429461 A CN201711429461 A CN 201711429461A CN 107814440 A CN107814440 A CN 107814440A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
The invention discloses a bacteria-algae complexing agent for sewage treatment and a preparation method thereof, wherein the bacteria-algae complexing agent for sewage treatment comprises the following raw materials in parts by weight: 5-8 parts of microalgae harvest, 11-14 parts of compound bacterial liquid and 1-1.5 parts of activated carbon; the preparation method comprises the following steps: adding activated carbon into the compound bacterial liquid, and stirring for 1.5-2 hours to obtain a mixture A; and adding the microalgae collected matter into the mixture A, and uniformly stirring to obtain the bacteria-algae complexing agent for sewage treatment. The bacteria-algae complexing agent for sewage treatment provided by the invention can construct a bacteria-algae symbiotic system in the culture sewage, effectively remove nitrogen and phosphorus pollutants in the culture sewage, and reduce the COD value.
Description
Technical field
The present invention relates to aquaculture wastewater process field, more particularly to a kind of bacterium algae complexing agent and its system for sewage disposal
Preparation Method.
Background technology
With the large-scale development of China's livestock and poultry breeding industry, per annual meeting, the livestock and poultry cultivation excrement of nearly 1,000,000,000 tons of generation is dirty, to having
The water resource of limit causes serious threat.
At present, the processing mode of aquaculture wastewater mainly has two kinds：First, being handled using methane-generating pit anaerobic fermentation, but produce
The nutritive salt such as the nitrogen phosphorus in the biogas slurry of raw enormous amount still containing high concentration, with the propulsion of the Course of Urbanization, disappear
Receive biogas slurry arable land it is increasingly insufficient, caused biogas slurry is directly discharged in water body, it will causes natural water serious eutrophication；
Second, bioanalysis removes, biological treatment is the pollutant in sewage to be degraded using microorganisms such as bacterium, fungies and/or together
Change and absorb, realize the purification of sewage, but due to aquaculture wastewater by Anaerobic Treatment it is latter as available carbon source it is too low, can not
Meet the needs of heterotrophism function bacterium, therefore to nitrate and phosphatic poor processing effect, it is necessary to physical filtering and chemisorbed
It is used in combination Deng various ways, this not only adds processing cost, also increases potential secondary pollution risk.
Microalgae facture is sewage treatmentmethod emerging at present, the abundant nitrogen contained in livestock and poultry cultivation sewage, phosphorus etc.
Nutriment can provide nutrition for microalgae, and handling sewage using microalgae can join with the large-scale production phase lotus root of microalgae simultaneously, realize
Resource recycles.
With microalgae purifying water effect and algae and microorganism, repercussion study is goed deep into water body, helotism system
Application on livestock and poultry cultivation sewage has obtained common concern.Helotism system be by algae to nitrogen and phosphorus thing in sewage and
The intake of organic matter removes a kind of mutualism system that effect contaminant degradation ability powerful with microorganism effectively combines
System.Therefore, the present invention proposes a kind of bacterium algae complexing agent for sewage disposal, and the bacterium algae complexing agent is put into aquaculture wastewater
In, you can build the helotism system for handling aquaculture wastewater.
The content of the invention
Based on technical problem existing for background technology, the present invention propose a kind of bacterium algae complexing agent for sewage disposal and
Its preparation method, helotism system can be built in aquaculture wastewater provided by the present invention for the bacterium algae complexing agent of sewage disposal
System, the nitrogen and phosphorus pollution thing in aquaculture wastewater is effectively removed, reduces COD value, the preparation method of bacterium algae complexing agent provided by the invention
Simple to operate, energy consumption is low, is adapted to commercial Application.
The invention provides a kind of bacterium algae complexing agent for sewage disposal, its raw material includes by weight：Microalgae recovery
Thing 5-8 parts, composite bacteria liquid 11-14 parts, activated carbon 1-1.5 parts.
Preferably, its raw material includes by weight：6.4 parts of microalgae recovery thing, 12.7 parts of composite bacteria liquid, 1.2 parts of activated carbon.
Preferably, the preparation method of microalgae recovery thing comprises the following steps：
S1, under aseptic technique, by chlorella vulgaris with 1 × 106Individual/mL-1 × 107Individual/mL inoculum concentration is inoculated into training
Support in base, be 25-27 DEG C in temperature, under the conditions of rotating speed is 110-130 revs/min, light dark cycle alternate culture 60-75 is small
When, obtain micro algae culturing liquid；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.1-5.4, adds the 1.5%- of micro algae culturing liquid weight
1.7% sodium citrate, 20-30 minutes are stirred, add the 3.8%-4.3% of micro algae culturing liquid weight calcium chloride, stir 40-50
Minute, centrifuged with 2000-2200 revs/min of revolution, obtain microalgae recovery thing.
Preferably, the raw material of culture medium includes according to parts by weight in the preparation method S1 of microalgae recovery thing：1-2 part grapes
Sugar, 4-6 parts starch sugar, 0.01-0.03 parts zinc sulfate, 0.1-0.3 parts magnesium sulfate, 0.15-0.3 parts ammonium sulfate, 0.2-0.4 part phosphorus
Acid dihydride potassium, 0.1-0.3 parts sodium nitrate, 9-12 parts sodium chloride and 240-260 part deionized waters.
Preferably, in the preparation method S1 light dark cycle alternate cultures of microalgae recovery thing, cultivated under photoenvironment
Time be 8-10 hours, the time cultivated under dark surrounds is 11-13 hours, photoenvironment, dark surrounds cycle alternation
Carry out.
Preferably, in the preparation method S1 light dark cycle alternate cultures of microalgae recovery thing, the illumination of photoenvironment is strong
Spend for 3500-3800Lux.
Preferably, composite bacteria liquid is prepared with the following method：After kitchen castoff is mixed with beet molasses, water, in 25-
30 DEG C ferment 15-20 days, and the liquid being filtrated to get is composite bacteria liquid.
Preferably, in composite bacteria liquid preparation method, kitchen castoff, beet molasses, the weight ratio of water are 1:0.2-0.3:
5-6。
It is used for the preparation method of the bacterium algae complexing agent of sewage disposal present invention also offers this kind, comprises the following steps：
Step 1: activated carbon is added in composite bacteria liquid, 1.5-2 hours are stirred, obtain mixture A；
Step 2: microalgae recovery thing is added in the mixture A of step 1 preparation, it is uniformly mixing to obtain for sewage disposal
Bacterium algae complexing agent.
Helotism system can be built in aquaculture wastewater provided by the present invention for the bacterium algae complexing agent of sewage disposal,
The helotism system is aided with microorganism to organic matter degradation, is effectively removed aquaculture wastewater based on the assimilation of algae
In nitrogen and phosphorus pollution thing, reduce COD value, adapt to, in various complicated growing environments, have broad application prospects.This hair
The preparation method of the bacterium algae complexing agent of bright offer is simple to operate, and energy consumption is low, is adapted to commercial Application.
Microalgae in bacterium algae complexing agent provided by the invention using its in growth course to organic matter in aquaculture wastewater,
The absorption of nutrient ingredients such as nitrogen, phosphorus, own cells skeleton is synthesized to purify livestock and poultry cultivation sewage, and can remove removing heavy metals and difficulty
Degradation of organic substances.
The preparation method of microalgae recovery thing mainly includes two parts of culture and harvesting of chlorella vulgaris in the present invention.It is general
The culture of logical chlorella uses light dark cycle alternate culture method, and preferred photoenvironment, dark surrounds incubation time,
After light dark cycle alternate culture 60-75 hours, terminate culture.Invention technician is had found when cultivating 60-75 hours
Between terminate chlorella vulgaris that culture obtains in section after livestock and poultry cultivation sewage is entered, its speed grown is better than in other times
Terminate the chlorella vulgaris that culture obtains in section.The harvesting of chlorella vulgaris adds lemon by adjusting micro algae culturing liquid pH value
Sour sodium, the mode of calcium chloride make chlorella flocculate and then separated from liquid, and this method is simple to operate, and energy consumption is low, are adapted to
Commercial Application.Have after sewage is put into that micro algae growth is rapid, nitrogen phosphorus uptake is big using the chlorella vulgaris that this method obtains,
The advantages that pollutant elimination efficiency is high.
The present invention prepares composite bacteria liquid using kitchen castoff, beet molasses, develops kitchen castoff, beet molasses one
The new utilization ways of kind.Containing the strains such as lactic acid bacteria, saccharomycete and their tunning in composite bacteria liquid, can decompose or
Absorb the pollutants such as the organic matter in aquaculture wastewater.
The present invention is used to adsorb bacterium and microalgae cell in bacterium algae complexing agent using activated carbon, makes bacterium and microalgae cell
Relatively steady state is in sewage, reduces the loss of microorganism.Activated carbon is first mixed with composite thallus, absorption is compound
Bacterium in bacterium solution, adds microalgae recovery thing, and microalgae cell replaces position of the part bacterium in activated carbon, helotism system
State of the system in relative equilibrium.
Embodiment
Below, technical scheme is described in detail by specific embodiment.
Embodiment 1
The invention provides a kind of bacterium algae complexing agent for sewage disposal, its raw material includes by weight：Microalgae recovery thing 6.4
Part, 12.7 parts of composite bacteria liquid, 1.2 parts of activated carbon.
Microalgae recovery thing is prepared using following methods：
S1, under aseptic technique, by chlorella vulgaris（Chlorella vulgaris）With 5.4 × 106Individual/mL inoculation
Amount is inoculated into culture medium, is 26 DEG C in temperature, under the conditions of rotating speed is 122 revs/min, is carried out light dark cycle and is alternately trained
Support, cultivated 9 hours, intensity of illumination 3700Lux under photoenvironment, the time cultivated under dark surrounds is 11 hours, light
After environment, dark surrounds cycle alternation culture 68 hours, micro algae culturing liquid is obtained；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.2, adds 1.58% lemon of micro algae culturing liquid weight
Sour sodium, stir 25 minutes, add 4.1% calcium chloride of micro algae culturing liquid weight, stir 44 minutes, with 2100 revs/min turn
Number centrifuges, and obtains microalgae recovery thing.
Wherein, the raw material of culture medium includes according to parts by weight in S1：1.6 part glucose, 5.2 portions of starch sugars, 0.02 part of sulphur
Sour zinc, 0.19 part of magnesium sulfate, 0.23 part of ammonium sulfate, 0.28 part of potassium dihydrogen phosphate, 0.21 part of sodium nitrate, 11 parts of sodium chloride and 250
Part deionized water.
Composite bacteria liquid is prepared with the following method：After kitchen castoff is mixed with beet molasses, water, in 27 DEG C of fermentations 18
My god, the liquid being filtrated to get is composite bacteria liquid.Wherein, kitchen castoff, beet molasses, the weight ratio of water are 1:0.28:
5.4。
Embodiment 2
The bacterium algae complexing agent for sewage disposal of embodiment 1, its raw material can also include by weight：Microalgae recovery thing 7.7
Part, 12.1 parts of composite bacteria liquid, 1.4 parts of activated carbon.
Embodiment 3
The bacterium algae complexing agent for sewage disposal of embodiment 1, its raw material can also include by weight：Microalgae recovery thing 5.3
Part, 13.8 parts of composite bacteria liquid, 1.1 parts of activated carbon.
Embodiment 4
The bacterium algae complexing agent for sewage disposal of embodiment 1, its raw material can also include by weight：Microalgae recovery thing 7.1
Part, 11.2 parts of composite bacteria liquid, 1.3 parts of activated carbon.
Embodiment 5
The bacterium algae complexing agent for sewage disposal of embodiment 1, microalgae recovery thing can also use following methods to prepare：
S1, under aseptic technique, by chlorella vulgaris with 1.6 × 106Individual/mL inoculum concentration is inoculated into culture medium,
Temperature is 25 DEG C, under the conditions of rotating speed is 128 revs/min, carries out light dark cycle alternate culture, 10 are cultivated under photoenvironment
Hour, intensity of illumination 3600Lux, the time cultivated under dark surrounds is 12 hours, and photoenvironment, dark surrounds circulation are handed over
After culture 73 hours, micro algae culturing liquid is obtained；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.3, adds 1.67% lemon of micro algae culturing liquid weight
Sour sodium, stir 22 minutes, add 3.9% calcium chloride of micro algae culturing liquid weight, stir 48 minutes, with 2200 revs/min turn
Number centrifuges, and obtains microalgae recovery thing.
Wherein, the raw material of culture medium includes according to parts by weight in S1：1.9 parts of glucose, 4.3 portions of starch sugars, 0.01 part of sulphur
Sour zinc, 0.12 part of magnesium sulfate, 0.17 part of ammonium sulfate, 0.38 part of potassium dihydrogen phosphate, 0.27 part of sodium nitrate, 10 parts of sodium chloride and 256
Part deionized water.
Embodiment 6
The bacterium algae complexing agent for sewage disposal of embodiment 1, microalgae recovery thing can also use following methods to prepare：
S1, under aseptic technique, by chlorella vulgaris with 8.9 × 106Individual/mL inoculum concentration is inoculated into culture medium,
Temperature is 27 DEG C, under the conditions of rotating speed is 113 revs/min, carries out light dark cycle alternate culture, 8 are cultivated under photoenvironment
Hour, intensity of illumination 3800Lux, the time cultivated under dark surrounds is 13 hours, and photoenvironment, dark surrounds circulation are handed over
After culture 62 hours, micro algae culturing liquid is obtained；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.1, adds 1.51% lemon of micro algae culturing liquid weight
Sour sodium, stir 28 minutes, add 4.2% calcium chloride of micro algae culturing liquid weight, stir 41 minutes, with 2000 revs/min turn
Number centrifuges, and obtains microalgae recovery thing.
Wherein, the raw material of culture medium includes according to parts by weight in S1：1.2 parts of glucose, 5.8 portions of starch sugars, 0.03 part of sulphur
Sour zinc, 0.27 part of magnesium sulfate, 0.28 part of ammonium sulfate, 0.23 part of potassium dihydrogen phosphate, 0.14 part of sodium nitrate, 9 parts of sodium chloride and 241 parts
Deionized water.
Embodiment 7
The bacterium algae complexing agent for sewage disposal of embodiment 1, composite bacteria liquid are prepared with the following method：By kitchen castoff with
After beet molasses, water mixing, fermented 19 days at 28 DEG C, the liquid being filtrated to get is composite bacteria liquid.Wherein, kitchen castoff,
Beet molasses, the weight ratio of water are 1:0.23:5.7.
Embodiment 8
The bacterium algae complexing agent for sewage disposal of embodiment 1, composite bacteria liquid are prepared with the following method：By kitchen castoff with
After beet molasses, water mixing, fermented 17 days at 26 DEG C, the liquid being filtrated to get is composite bacteria liquid.Wherein, kitchen castoff,
Beet molasses, the weight ratio of water are 1:0.25:5.1.
Embodiment 9
The invention provides a kind of preparation method of the bacterium algae complexing agent for sewage disposal, comprise the following steps：
Step 1: 1.2 kilograms of activated carbons are added in 12.7 kilograms of composite bacteria liquids, stir 1.8 hours, obtain mixture A；
Step 2: 6.4 kilograms of microalgae recovery things are added in the mixture A of step 1 preparation, it is uniformly mixing to obtain for dirt
The bacterium algae complexing agent of water process.
Embodiment 10
The invention provides a kind of preparation method of the bacterium algae complexing agent for sewage disposal, comprise the following steps：
Step 1: 1.4 kilograms of activated carbons are added in 12.1 kilograms of composite bacteria liquids, stir 1.6 hours, obtain mixture A；
Step 2: 7.7 kilograms of microalgae recovery things are added in the mixture A of step 1 preparation, it is uniformly mixing to obtain for dirt
The bacterium algae complexing agent of water process.
The chlorella vulgaris being inoculated into the present invention in the preparation method S1 of microalgae recovery thing in culture medium is preferably
Activation method well known by persons skilled in the art, preferably slant activation method can be selected in the chlorella vulgaris activated, activation method；
Micro algae culturing liquid pH regulation can preferably add the hydrochloric acid that mass fraction is 30%-40% by the way of hydrochloric acid is added in S2.
The methods of inoculation that is related in the present invention, stirring, centrifugation, configuration of culture medium be this area it is known often
Know, will not be repeated here.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (9)
1. a kind of bacterium algae complexing agent for sewage disposal, it is characterised in that its raw material includes by weight：Microalgae recovery thing 5-
8 parts, composite bacteria liquid 11-14 parts, activated carbon 1-1.5 parts.
2. a kind of bacterium algae complexing agent for sewage disposal according to claim 1, it is characterised in that its raw material is by weight
Part includes：6.4 parts of microalgae recovery thing, 12.7 parts of composite bacteria liquid, 1.2 parts of activated carbon.
A kind of 3. bacterium algae complexing agent for sewage disposal according to claim 1, it is characterised in that the microalgae recovery
The preparation method of thing comprises the following steps：
S1, under aseptic technique, by chlorella vulgaris with 1 × 106Individual/mL-1 × 107Individual/mL inoculum concentration is inoculated into training
Support in base, be 25-27 DEG C in temperature, under the conditions of rotating speed is 110-130 revs/min, light dark cycle alternate culture 60-75 is small
When, obtain micro algae culturing liquid；
S2, the pH value of the micro algae culturing liquid obtained in S1 is adjusted to 5.1-5.4, adds the 1.5%- of micro algae culturing liquid weight
1.7% sodium citrate, 20-30 minutes are stirred, add the 3.8%-4.3% of micro algae culturing liquid weight calcium chloride, stir 40-50
Minute, centrifuged with 2000-2200 revs/min of revolution, obtain microalgae recovery thing.
A kind of 4. bacterium algae complexing agent for sewage disposal according to claim 3, it is characterised in that the microalgae recovery
The raw material of culture medium includes according to parts by weight in the preparation method S1 of thing：1-2 parts glucose, 4-6 parts starch sugar, 0.01-0.03
Part zinc sulfate, 0.1-0.3 parts magnesium sulfate, 0.15-0.3 parts ammonium sulfate, 0.2-0.4 parts potassium dihydrogen phosphate, 0.1-0.3 part nitric acid
Sodium, 9-12 parts sodium chloride and 240-260 part deionized waters.
A kind of 5. bacterium algae complexing agent for sewage disposal according to claim 3, it is characterised in that the microalgae recovery
In the preparation method S1 light dark cycle alternate cultures of thing, the time cultivated under photoenvironment is 8-10 hours, in dark
The time cultivated under environment is 11-13 hours, and photoenvironment, dark surrounds cycle alternation are carried out.
A kind of 6. bacterium algae complexing agent for sewage disposal according to claim 5, it is characterised in that the microalgae recovery
In the preparation method S1 light dark cycle alternate cultures of thing, the intensity of illumination of photoenvironment is 3500-3800Lux.
A kind of 7. bacterium algae complexing agent for sewage disposal according to claim 1, it is characterised in that the composite bacteria liquid
Prepare with the following method：After kitchen castoff is mixed with beet molasses, water, ferment 15-20 days, filter at 25-30 DEG C
To liquid be composite bacteria liquid.
A kind of 8. bacterium algae complexing agent for sewage disposal according to claim 7, it is characterised in that the composite bacteria liquid
In preparation method, kitchen castoff, beet molasses, the weight ratio of water are 1:0.2-0.3:5-6.
9. the preparation method of a kind of bacterium algae complexing agent for sewage disposal, it is characterised in that comprise the following steps：
Step 1: activated carbon is added in composite bacteria liquid, 1.5-2 hours are stirred, obtain mixture A；
Step 2: microalgae recovery thing is added in the mixture A of step 1 preparation, it is uniformly mixing to obtain for sewage disposal
Bacterium algae complexing agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110590419A (en) * | 2019-09-29 | 2019-12-20 | 鄂尔多斯市王道酒业有限公司 | Fermentation treatment method of vinasse |
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Application publication date: 20180320 |