CN1087775C

CN1087775C - Tech. for culturing spirulina rich in selenium

Info

Publication number: CN1087775C
Application number: CN92112160A
Authority: CN
Inventors: 谢应先; 徐晶; 陈婉华
Original assignee: SOIL AND FERTILIZER INST CHINE
Current assignee: SOIL AND FERTILIZER INST CHINE
Priority date: 1992-10-16
Filing date: 1992-10-16
Publication date: 2002-07-17
Anticipated expiration: 2007-10-16
Also published as: CN1085951A

Abstract

The present invention relates to a sieving technology of spirulina strains with strong selenium enriching ability and a selenium enriching technology. Different spirulina species (strains) are taken for timely culture and acclimation through a sodium selenate gradient concentration culture solution to sieve out spirulina species with strong selenium enriching ability. Through the continuous culture or the concentration culture selenium enriching technology, organic selenium content in the spirulinae reaches 860 to 2100 ppm. The technology has the advantages of simple method, strong selenium enriching ability, low cost, etc. The selenium enriched spirulinae can be used for various diseases resulted from lack of selenium, ageing resistance, carcinogenesis resistance and prevention and cure of the keshan disease and the catarct, and has certain health care and therapy effects.

Description

The spirulina technique of enriched selenium

The present invention relates to a kind of spirulina technique of enriched selenium that is applied to protective foods, beverage and auxiliary pharmaceutical adjuvant.Spirullina is in the Spirullina of Cyanophyta, Cyanophyceae, hormogon[ium order, Oscillariaceae.The kind of having separated at present and being described has more than 35 approximately.The algae kind that is used to produce mainly is from African Chad and isolating spirulina plalensis in lake, Mexico tex Cork and spirulina maxim.Show according to chemical analysis, spirulina protein content is 58～70%, and contains multiple amino acids, has very high edibleness, up to this century the seventies just cause the concern of Chinese scholars, become the typical high-protein health nutritious prod that Food and Argriculture OrganizationFAO is recommended.Chinese patent CN1035425 " manufacture method of blue green alga-spiral alga drink " is to be that main raw material is through levigate, leaching, enzymatic hydrolysis with the blue green alga-spiral alga, obtain protein, amino acid solution, assistant produces blue transparent protein, amino acid beverage with other additive.Chinese patent CN1015357 " spirulina technology of intensifying zinc and iron ", utilize spirulina to reach under the suitable pH value at normal temperature, the low light level, zinc, iron ion in disposable a large amount of absorption, the enrichment solution, its complexing action is very remarkable, but protein, amino acid in the spirochete there is not influence, the product of producing can be used to make medicine, protective foods, and can be used as various zinc-iron nutritional additives.DE3421644 " the nutritious spirulina composition that contains selenium and V.E " has introduced the nutrition dietary prescription, adding ultimate density in the dry powder of spirulina is the Sodium Selenite of 20～200PPm, but the selenium of this fusion remains inorganic selenium, and the accumulation excessive amount all has certain murder by poisoning to people and animal.Along with science and technology development, selenium has been proved to be necessary a kind of important trace element in the vital movement, be the integral part that constitutes human body and the indispensable Selenoperoxidase of animal life metabolism, thereby it have the normal physiological function of keeping, enhance immunity function, suppress vital role such as carcinogens vigor and opposing toxic substance.Because the critical role of selenium in modern medicine and trophology, in recent years the domestic research of attaching great importance to this respect, contain selenium on the market, selenium-enriched food is come out one after another, as tea rich in selenium, rich selenium mushroom eat, egg rich in selenium, yeast rich in selenium etc., but these rich selenium products are not that selenium content is low, are exactly that rich selenium carrier itself is not ideal enough.

The objective of the invention is to adopt the bio-carrier of spirulina, a kind of spirulina technique of enriched selenium is provided, produce collection high protein and be rich in medicine, the foodstuff additive that organoselenium is an one as selenium.It is short that spirulina is bred fast growth cycle, and culture condition is less demanding, has the function of gathering trace element.Studies show that different its accumulation abilities of algae kind are different.The selenium content of general spirulina powder only be about 0.2PPm, however spirulina richness selenium concentration reach more than 500～1000PPm, higher use value is just arranged.In order to obtain the algae strain of high selenium ability, the contriver makes its method that progressively adapts to reach the purpose of domestication by changing the outside atmosphere of growth of spirulina platensis.

Spirulina technique of enriched selenium of the present invention is according under 3000～5000 lux conditions in 25～30 ℃ of temperature, PH8～10, optimal light, to in the Zarrouk nutrient solution, be placed in the nutrient solution of low selenium concentration 20～30PPm 3～5 months domestication time by cultured spirulina earlier.Initial frond is because of this kind of incompatibility culture condition, a large amount of frond death, the rubescent variable color of algae liquid.But the frond of surviving has progressively adapted to this kind culture condition through cultivation after a while, and can normal growth.After domestication, filter out the strong algae strain of selenium rich ability and produce the algae kind as rich selenium.Grow in nutrient solution when frond and to reach certain density (swimming in the liquid level slabbing), add Sodium Selenite, make the ultimate density of selenium reach 350～400ppm, make spirulina normal growth under higher selenium concentration, under suitable temperature, illumination condition, cultivated 5～8 days.Certainly the concentration of selenium can not unrestrictedly improve, reach certain concentration (574.20PPm) after, the spiral frond will be poisoned and all dead.From high selenium concentration (the different algae strains that acclimation and screening goes out 350～400PPm) nutritive mediums, cultured continuously under identical selenium concentration.After this filtered every 5～8 days and gather once, unnecessary inorganic selenium in the bright algae mud is rinsed out with clear water, wash to pH value be 7,60～80 ℃ of oven dry or spraying dryings down, become rich selenium algae powder then.

Also can use the method for cultivating rich selenium that concentrates in addition, will be from the high selenium concentration (frond that filters out 350～400PPm) nutrient solutions, or through the low selenium concentration (frond after 20～30PPM) domestications, placing selenium concentration is 700～1000PPM nutrient solution, under illumination≤3000 luxs, 25～30 ℃ of temperature, pH value 7.5～9 conditions, cultivated 2～24 hours, filtration is gathered, with clear water the unnecessary inorganic selenium in the rich selenium algae mud is rinsed out, being washed till pH value is 7, dry down or spraying drying at 60～80 ℃, be rich selenium spirulina powder.

Spirulina bacterial classification of the present invention is preserved, indoor propagation, all adopts the Zarrouk substratum, and its main component is (g/l);

Sodium bicarbonate 16.8 ferrous sulfate 0.0 1 dipotassium hydrogen phosphates 0.5

Vitriolate of tartar 1.0 SODIUMNITRATE 2.5 crystallization calcium chloride 0.04

Salt 1.0 A ₅Solution 1ml B ₆Solution 1ml

Sal epsom 0.2 PH 8.1

A ₅Solution (g/l)

Boric acid 2.86 manganous chloride 1.8 zinc sulfate 0.22 copper sulfate 0.08 cupric oxide 0.01

B ₆Solution (mg/l);

Ammonium vanadate 22.0 single nickel salts 47.8 sodium wolframates 17.9 titanium sulfates 40.0 Xiao Suangus 4.4

In the difference domestication stage at spirulina, Sodium Selenite is joined in the nutrient solution by its concentration consumption.

The distinct advantages of spirulina technique of enriched selenium of the present invention is:

1, since the spirulina that the present invention adopts be a kind of protein content up to 58～70%, amino acid is complete, and contains the edibility blue-green algae of multivitamin, pigment, very easily digested and assimilated again, thereby be comparatively ideal bio-carrier by animal body.

2, spirulina breeding is fast, and growth cycle is short, cultivates less demandingly, and individuality is bigger, and last buoyancy is good, is convenient to gather, so compare with several microorganisms that other has been used, has more that technology is easy, cost is low, the characteristics of high efficiency.

3, because present technique is to add a certain amount of Sodium Selenite in the nutrient solution of spirulina, make growth of spirulina platensis, in reproductive process, progressively absorb selenium salt, pass through biologically, make the selenium element fully with the Gelucystine in the frond internal protein, methionine(Met) combination, guarding against is real organoselenium (infrared absorption spectrum analysis of rich selenium algae powder has confirmed the generation of organoselenium), has increased edible safe reliability, thereby has had more edibleness.

4, the selenium-enriched algae kind that obtains by the rich selenium experiment sieving of different algae kinds can make the interior organic selenium content of frond reach 860～2100PPM, and this has just improved rich selenium amount greatly, thereby has improved the economic worth of rich selenium spirulina as additive.

The rich selenium spirulina that the present invention produced can be used for the additive of protective foods, beverage and medicine as a kind of source of organoselenium, and cancer, heart trouble, Keshan disease, cataract, atherosclerosis are all had certain prevention and auxiliary curative effect.

Description of drawings:

Fig. 1 spirulina technique of enriched selenium process flow diagram

Fig. 2 spirulina powder infrared analysis

Embodiment:

1, the rich selenium method of cultured continuously adds 3000ml Zarrouk nutrient solution in plastic big basin, inoculates selenium-enriched spirulina kind by 5% inoculum size, cultivates 5～6 days (in time keep the skin wet, keep nutrient solution volume 3000ml) under suitable temperature light condition.When spirulina is bred to certain density, when on liquid level, floating one deck, add again after 2.40g Sodium Selenite (final selenium concentration be 368PPM) fully stirs evenly, continue to cultivate 5～6 days, then algae liquid is poured into (filtrate recovery inoculates SPIRULINA CULTIVATION) on the 250 order nylon mesh screens, the abundant drip washing of bright algae mud to the wash water pH value that will gather in the crops with clear water reaches 7, filter is done, move in the culture dish, oven dry can obtain rich selenium spirulina.

2, concentrate the rich selenium method of cultivating, to put into the bright algae mud 1000g that 250 order nylon mesh screens are gathered and fill the 1000ml sodium selenite solution (final selenium concentration is in 500～1000PPM) the plastic big basin, fully to stir evenly, under the normal temperature illumination condition, after leaving standstill 4～24 hours, filter,, the unnecessary inorganic selenium in frond surface is washed out with the bright algae mud of the abundant drip washing of clear water, make the wash water pH value reach at 7 o'clock, filter is done, and after the oven dry, promptly obtains rich selenium spirulina.

Claims

1, a kind of spirulina technique of enriched selenium, it is characterized in that the spirulina kind of in the Zarrouk substratum, turning out, at first domestication is cultivated in the nutrient solution of low selenium concentration, filter out the frond that adapts to this kind nutrient solution, then, place high selenium concentration nutrient solution to carry out the screening of selenium-enriched algae kind, cultured continuously is carried out in the algae strain that sieve is sent under identical selenium concentration; Maybe the algae strain of selecting being placed selenium concentration is 700～1000ppm nutrient solution, incubation time concentrated cultivation in 2～24 hours, and the rich selenium algae mud that obtains is washed unnecessary inorganic selenium off with clear water, wash PH=7, become rich selenium algae powder in 60～80 ℃ of following oven dry of temperature or spraying drying.

2, technique of enriched selenium according to claim 1 is characterized in that described domestication and culture condition, and optimum temperature is 25～30 ℃, illumination 3000～5000 luxs, and PH 8～10.

3, technique of enriched selenium according to claim 1 is characterized in that described algae kind domestication, is that the selenous acid sodium salt is added in the Zarrouk nutrient solution by its concentration, and low selenium concentration is 20～30ppm, and the domestication time is 3～5 months.

4, technique of enriched selenium according to claim 1, it is characterized in that the screening of described selenium-enriched algae kind, its high selenium nutrient solution selenium concentration is 350～400ppm, incubation time is 5～8 days, involve continuous the cultivation in a criminal case in the algae of selecting thereafter and under identical conditions, carry out, taked once rich selenium algae mud every 5～8 days.

5, technique of enriched selenium according to claim 1 is characterized in that described concentrated cultivation, also can be the frond after low selenium concentration domestication, places 700～1000ppm selenium nutrient solution to cultivate, and incubation time is 2～24 hours.