CN1063786C - Manufacturing method of iron-rich pure spirulina - Google Patents
Manufacturing method of iron-rich pure spirulina Download PDFInfo
- Publication number
- CN1063786C CN1063786C CN95109095A CN95109095A CN1063786C CN 1063786 C CN1063786 C CN 1063786C CN 95109095 A CN95109095 A CN 95109095A CN 95109095 A CN95109095 A CN 95109095A CN 1063786 C CN1063786 C CN 1063786C
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- CN
- China
- Prior art keywords
- iron
- spirulina platensis
- rich
- spirulina
- ferrum
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- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a method for producing spirulina platensis enriching iron, particularly to a culture medium with a reasonable formula, and culture technique. The ferrous methionine in a culture medium is easy to absorb and utilize; after spirulina platensis is cultivated for 12 hours under the conditions that the light intensity is from 2400 to 2600LX and the temperature is from 30 to 38 DEG C, the content of the iron enriched in the spirulina platensis reaches 7000 ug/g(dry weight). The spirulina platensis enriching iron can be used as biomedical raw material for treating iron-deficiency anemia of human bodies, and can be used as health food and beverages for preventing the iron-deficiency anemia. The spirulina platensis enriching iron has the effects of shore production period, low production cost and high iron content, and is suitable for industrialized production.
Description
The present invention relates to the artificial culture of algae, particularly relate to the production method of a kind of spirulina plalensis (SpirulinaPlatensis) enrichment iron.
Spirulina is a kind of alkaline waters that is grown in, and form such as spiral helicine phycophyta belong to Cyanophyta on taxonomy, Cyanophyceae, hormogon[ium order, a genus-Spirullina in the Oscillariaceae.Spirulina plalensis is a kind of Spirullina.
Spirulina contains rich nutrient substances, and wherein protein content is up to 60-70%, and amino acid whose composition is very reasonable in the protein, has necessary eight seed amino acids of human body.Therefore in addition, also be rich in multivitamin, mineral substance, chlorophyll, carotene, unsaturated fatty acids and polysaccharose substance, and nontoxic easy digestion, be described as human classic food, had the wide development prospect.
In the contained mineral substance of spirulina, the necessary iron of human body (Fe) is arranged, the content of calcium (Ca), phosphorus (P) and zinc (Zn), particularly iron, iron content 600ug in every gram dry algae powder, the spirulina iron-holder of utilizing the Zarrouk substratum to produce is 800-1000ug/g (dry weight).See for details
Zarrouk?c；1966，Contribution?al?etude?dune?cyaniophcee?influence?de?di-vens?facteurs?Physiques?et?chimiques?sur?la，?Croissance?et?la?Photosynthesede?Spirulina?maxima?(Setch.et?Gardner)?Geitler?Ph.D.thesis，University?ofPairs，France.。
U.S. Earthrise Farms company increases in the Zarrouk substratum for iron, and producing the spirulina powder iron-holder is 1500ug/g (in heavy).See for details
Robent?Henrikson，1989.Earth?Food?Spirulina.P.36.Ronere?Enter-prises，Inc.
The iron-holder of the algae powder that the method for above-mentioned document introduction is produced, production efficiency and production cycle still are difficult to reach the requirement that rich iron algae powder is produced in industrialization.
The human body iron deficiency can cause iron deficiency anemia, and this disease is very general in China women, children and the elderly, and the medicine commonly used for the treatment of this disease at present is oral ferrous sulfate, can cause gastrointestinal side effect but take medicine for a long time, and absorption rate is low.
The objective of the invention is to propose a kind of novel technical method of preventing and treating the biological medicine source of iron deficiency anemia.Promptly produce the spirulina plalensis algae powder of Fu Tie,, and treat this sick biomedicinal raw material for the protective foods and the beverage of manufacturer production at iron deficiency anemia.
For achieving the above object, its technical scheme is:
Spirulina plalensis is seeded in the substratum, and when the OD value reaches 1.0, the pH value is 10.5 o'clock, add a certain amount of easy absorbed ferrous methionine, at low light level 2400-2600Lx, cultivated 12 hours down, just can obtain the spirulina plalensis algae powder of iron-holder for 30-38 ℃ up to 7000ug/g (dry weight).
The present invention compared with prior art has following advantage and effect
1. be enriched in the iron level height in the spirulina plalensis.High 7 times than with the ZShi culture medium culturing, than Earthrise Farms company produce high 4.6 times.
2. production cost reduces.The nutritive ingredient Fe-EDTA of substratum has reduced 3/4ths, NaNO
3Amount has reduced 2g/L.
3. cultivate save energy with the low light level.Incubation time shortens, only 12 hours production phase.
Realize best way of the present invention:
1. culture medium prescription (g/L)
NaHCO
3 16.8
NH
3H
2O: NaNO
3=1: 4 total nitrogen 100mgN/L
NaCl 1.0
K
2SO
4 1.0
KH
2PO
4 0.5
FeSO
4·7H
2O 0.0025
EDTA·Na
2 0.02
MgSO
4·7H
2O 0.2
CaCl
2·2H
2O 0.04
A
5 1ml
B
6 1ml
Distilled water 1000ml
pH 8.5-9。
2. production stage: several steps in the following order carries out.
Washing, drying → Spirulina Platensis rich in Ferrum algae powder.
Spirulina plalensis is seeded in stir culture in the substratum, reaches 1.0, when the pH value reaches 10.5, adds ferrous methionine in culture, add-on is 1.08 * 10 when being cultured to the OD value
-3Mol/l.
At temperature 30-38 ℃, continue to be cultured to 12 hours under the intensity of illumination 2400-2600LX and collect the algae powder, spray-dried, obtain the spirulina plalensis dry powder of rich iron, water content 5-7%.
Claims (3)
1. the production method of Spirulina Platensis rich in Ferrum is characterized in that used substratum is by N, P, C, K, Na, Mg, Ca, A
5, B
6, the EDTA-Fe nutritive element forms, in the training period, when OD value reaches 1.0, pH value reaches at 10.5 o'clock, presses adding ferrous methionine 1.08 * 10 in every liter of substratum
3Mol is 30-38 ℃ in temperature, intensity of illumination be under the 2400-2600Lx condition through 12 hours cultivation, regather, wash, the dry Spirulina Platensis rich in Ferrum dry algae powder that obtains.
2. the production method of Spirulina Platensis rich in Ferrum according to claim 1 is characterized in that the composition content of substratum is (g/L)
NaHCO
3 16.8
NH
3H
2O: NaNO
3=1: 4 total nitrogen 100mgN/L
NaCl 1.0
K
2SO
4 1.0
KH
2PO
4 0.5
FeSO
4·7H
2O 0.0025
EDTA·Na
2 0.02
MgSO
4·7H
2O 0.2
CaCl
2·2H
2O 0.04
A
5 1ml
B
6 1ml
Distilled water 1000ml
pH 8.5-9。
3. the production method of Spirulina Platensis rich in Ferrum according to claim 1 is characterized in that the water content of the Spirulina Platensis rich in Ferrum dry powder that obtains is 5-7%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95109095A CN1063786C (en) | 1995-09-05 | 1995-09-05 | Manufacturing method of iron-rich pure spirulina |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95109095A CN1063786C (en) | 1995-09-05 | 1995-09-05 | Manufacturing method of iron-rich pure spirulina |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1144844A CN1144844A (en) | 1997-03-12 |
| CN1063786C true CN1063786C (en) | 2001-03-28 |
Family
ID=5077055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95109095A Expired - Fee Related CN1063786C (en) | 1995-09-05 | 1995-09-05 | Manufacturing method of iron-rich pure spirulina |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1063786C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101492644B (en) * | 2008-12-04 | 2013-06-05 | 上海化工研究院 | Method for biosynthesis of 15N mark helix alga |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533557B (en) * | 2010-12-22 | 2014-09-10 | 丽江程海保尔生物开发有限公司 | Overwintering, rejuvenation and preservation culture medium for cultivating spirulina |
| CN102586491B (en) * | 2012-03-02 | 2013-08-07 | 云南绿A生物工程有限公司 | Method for controlling heavy metals in process of culturing spirulina |
| CN102845781B (en) * | 2012-09-01 | 2014-05-14 | 山东博然螺旋藻生物股份有限公司 | Preparation method of chrome-rich instant spiral algae |
| CN102793216B (en) * | 2012-09-12 | 2013-09-18 | 北京林业大学 | Method for preparing spiral seaweed powder by utilizing microwave vacuum freeze drying |
| CN104450581A (en) * | 2014-12-10 | 2015-03-25 | 安徽大学 | Cultivation method of hpyerglycemic spirulina |
| CN105639495A (en) * | 2016-01-08 | 2016-06-08 | 烟台布鲁拜尔生物制药有限公司 | Preparation method of high-iron spirulina powder |
| CN109837234A (en) * | 2016-12-31 | 2019-06-04 | 北京林业大学 | A method of regulation nutritious spirulina element calcium element accumulation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1085951A (en) * | 1992-10-16 | 1994-04-27 | 中国农业科学院土壤肥料研究所 | The spirulina technique of enriched selenium |
| CN1092104A (en) * | 1994-01-30 | 1994-09-14 | 曹吉祥 | The preparation method of germanium and selenium spirulina |
-
1995
- 1995-09-05 CN CN95109095A patent/CN1063786C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1085951A (en) * | 1992-10-16 | 1994-04-27 | 中国农业科学院土壤肥料研究所 | The spirulina technique of enriched selenium |
| CN1092104A (en) * | 1994-01-30 | 1994-09-14 | 曹吉祥 | The preparation method of germanium and selenium spirulina |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101492644B (en) * | 2008-12-04 | 2013-06-05 | 上海化工研究院 | Method for biosynthesis of 15N mark helix alga |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1144844A (en) | 1997-03-12 |
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