KR101783020B1 - Composition for Treating Sewage and a Method for Treating Sewage Using the Same - Google Patents

Abstract

The present invention discloses a composition for purifying a purifier and a method for purifying a purifier using the same. Specifically, the present invention discloses a composition for the purification of pollutants using these microbial cultures obtained by adding collagen or collagen and / or GABA at the time of culturing photosynthetic bacteria, lactic acid bacteria and / or yeast, and a method for purifying pollutants using the same.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for purifying feces and a method for purifying feces,

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a composition for purifying a purifying agent and a method for purifying the purifying agent.

Water quality continues to deteriorate due to the massive discharge of industrial wastewater, domestic sewage, agricultural and livestock wastewater, and so on due to industrial development, human population densification, and large scale livestock industry.

The water pollution caused by the scrap is ultimately given to humans through the food chain. It is caused by the accumulation of cadmium in the body caused by accumulation of mercury in the body. Is often mentioned as a representative example.

Techniques for purifying waste materials can be largely divided into technologies using devices, and technologies using microorganisms, natural substances, and chemical substances.

As a technique for purifying wastes using the preparation, silver nitrate (AgNO3), gold chloride (AuCl3), borax (Na2B4O7), sodium carbonate (NaCO3), sodium peroxide (Na2O2) (Korean Patent Publication No. 2002-0023823), a technique using calcium ascorbate and dried coral algae (Korea Patent Publication No. 2002-0070983), and a technique using red mud (Korean Patent No. 2003-0074308), a microorganism having an ability to degrade ethylene glycol (Accession No. KCTC10177BP, Korean Patent Publication No. 2003-0074018), a new strain microorganism belonging to Massilia sp. (Accession No. KACC91486P, 1007843), and the like can be exemplified.

In addition, there is an example of using loess for red tide and green tide treatment (Korean Patent Laid-Open Publication No. 1998-033476), and an example of using silica (silage) for manure or livestock wastewater (Japanese Patent Laid-Open Publication No. 2001-179284).

The present invention discloses a purification technique of a waste matter using a culture of a combination of lactic acid bacteria, photosynthetic bacteria and / or yeast.

An object of the present invention is to provide a composition for cleaning a waste.

Another object of the present invention is to provide a method for purifying a waste.

As shown in the following examples and experiments, the inventors of the present invention found that these collagen particles are formed when collagen is added during the cultivation of lactic acid bacteria, photosynthetic bacteria and / or yeast, and in particular, collagen particles and GABA The inventors of the present invention found that the culture of these microbial conjugates lowered the COD, BOD, total nitrogen (TN) and total phosphorus (TP) of stream water to improve water quality It was confirmed that ammonia, methyl mercaptan, hydrogen sulfide and trimethylamine in livestock wastewater were removed to improve odor, and cadmium, nickel, chromium and zinc in livestock wastewater were reduced to remove heavy metals.

On the other hand, when the culture obtained without addition of collagen or GABA at the time of culturing lactic acid bacteria, photosynthetic bacteria and / or yeast was set as a control group and the evaluation of the water quality improving activity, odor improving activity and heavy metal improving activity of these mixed cultures These activities were found to be significantly lower than the activity of the resulting microbial conjugate cultures to which collagen or collagen and GABA were added.

The present invention has been completed on the basis of these experimental results.

In one aspect, the present invention relates to a composition for cleaning a waste matter.

The composition for cleaning pollution of the present invention is characterized in that the microorganism culture obtained by the method for producing a microorganism culture comprising the following steps (a) to (c) is contained as an active ingredient:

(a) preparing a culture medium for culturing two or more microorganisms of photosynthetic bacteria, lactic acid bacteria and yeast,

(b) inoculating and culture two or more microorganisms of photosynthetic bacteria, lactic acid bacteria and yeast in said medium, and

(c) adding collagen particles during preparation of the medium of step (a) or during culture of step (b)

As used herein, the term "active ingredient" alone refers to a substance which exhibits the desired active purgative cleansing activity or itself exhibits activity together with a carrier which is not active, or a carrier itself, ≪ / RTI > as used herein refers to a component capable of exhibiting higher activity as compared to when used as < RTI ID = 0.0 >

In the present specification, the term " purifying waste "means that the indicator indicating the contaminated condition of the waste material changes to a state showing a good state. Here, referring to the following experimental example, "indicator indicating pollution state" means COD, BOD, TN and / or TP indicating the state of water quality, gases causing odor such as ammonia, methyl mercaptan, hydrogen sulfide, (Or concentration) such as trimethylamine, and the content (or concentration) of heavy metals such as cadmium, nickel, chromium, and zinc. Therefore, the fact that the indicator indicating the pollution state of the pollution state changes to a state showing a good state means that COD, BOD, TN or TP in the pollution is lowered, the gas content (or concentration) Or the content (or concentration) of heavy metals in the waste material is lowered.

In the present specification, the term "scrap" means any thing that is directly or indirectly useful to a human by improving its contamination status. Therefore, if the pollution conditions such as river water, sea, river, ground water, drinking water need to be improved as well as severe pollution such as factory wastewater, animal wastewater, domestic wastewater, sewage, etc. If any) is included in the definition of the waste.

In this specification, the term "microorganism culture" means not only the culture stock solution itself but also a liquid concentrate (i.e., a concentrate) or a powder concentrate obtained by concentrating the culture stock solution.

On the other hand, photosynthetic bacteria generally refers to bacteria that perform carbon assimilation by using light energy like higher plants. These photosynthetic bacteria are chloroblue bacteria sp . ), Chromatium bacterium ( Chromatium sp . ), Rhodospirillum ( Rhodospirillum sp . ), Rhodopsendomonas bacteria ( Rhodopsendomonas sp . ), Rhodobacter sp . ), Rhodopseudomonas bacteria ( Rhodopseudomonas sp . ), And all of these photosynthetic bacteria can be used in the production of the microorganism culture as an active ingredient of the present invention. As the photosynthetic bacteria that can be used in consideration of the following examples, Rhodopseudomonas sp . ), In particular Rhodopseudomonas palustris .

Lactic acid bacteria means a microorganism that decomposes saccharides such as glucose to produce lactic acid. In the present specification, lactic acid bacteria are lactobacillus ( Lactobacillus sp . ), Streptococcus lactis bacteria ( Streptococcus sp . ), Pediococcus ( Pediococcus sp . ), Lactobacillus lactic acid bacteria ( Leuconostoc sp . ) And Bifidobacterium ( Bifidobacterium < RTI ID = 0.0 > sp . ). Specific examples thereof include Lactobacillus ( Lactobacillus &lt; RTI ID = 0.0 &gt; acidophilus , Lactobacillus plantarum , Lactobacillus &lt; RTI ID = 0.0 &gt; fermentum , Bifidobacterium longum), Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum ) and the like. All of these lactic acid bacteria can be used in the method of the present invention. However, Lactobacillus acidophilus or Lactobacillus plantarum is preferably used in the following <Examples>, and it is preferable to use Lactobacillus bacteria in the genus Lactobacillus, If you use it.

Any yeast that can be used in the production of the microorganism culture as an active ingredient of the present invention may be any yeast of the genus Saccharomyces. Thus, Saccharomyces the rouxii), Seth Serenity My Vichy as Saccharomyces (Saccharomyces cereviciae), access to the MY Ob pole miss Saccharomyces (Saccharomyces oviformis , Saccharomyces steineri and the like can all be used. Preferably, the case of using saccharomyces cerevisiae used in the following examples is used.

The lactic acid bacteria, yeast and photosynthetic bacteria used in the production of the microorganism culture as the active ingredient of the present invention may be purchased from biotechnology related companies or purchased from domestic or foreign microorganism depository institutions such as the genetic bank of the biotechnology research institute, Microorganism Conservation Center, American Type Culture Collection (ATCC)). If necessary, the strain may be directly used.

The collagen used in the production of the microorganism culture as an active ingredient of the present invention acts as a mediator in the formation of a conjugate by photosynthetic bacteria, lactic acid bacteria and / or yeast. As a result, the culture of the microorganism- Which is significantly higher than that of the obtained microorganism culture.

Collagen is a protein that constitutes cartilage, tendon, etc. in the body of mammals and is a protein involved in intercellular binding. The inventors of the present invention have used collagen as a mediator in the formation of microbial conjugates by focusing on these characteristics of collagen. Even if the photosynthetic bacteria, lactic acid bacteria and / or yeast can be cultivated jointly (that is, even if they form a symbiotic relationship), collagen is not obtained when collagen is not added to the medium, and as shown in the following experimental examples, And thus the cleansing activity is markedly lowered. Therefore, the use of collagen in the production of a microorganism culture as an active ingredient of the present invention can be said to be an additive that must be added in order to obtain a microbial conjugate and, as a result, to increase the activity of purifying the microorganism. Such collagen may be used after being inoculated with photosynthetic bacteria, lactic acid bacteria, and / or yeast in a culture medium, and then added to the culture medium or added at the time of preparing the culture medium. When added after culturing, it is preferably added about 12 hours after the start of the culture. The amount of collagen to be added may in any case be added in the range of 0.3 to 2.0 parts by weight based on 100 parts by weight of the medium.

The collagen for use in the production of the microorganism culture as an active ingredient of the present invention can be used in the form of powder, and the smaller the particle size (particle size) of the collagen is, the more uniformly and easily the mixture is mixed with the medium, It is not possible to form a microbial complex even if the size is large. Usually, collagen powder having an average particle size in the range of 0.001 μm to 0.0005 mm will be used.

The culture medium for culturing the photosynthetic bacteria, lactic acid bacteria and / or yeast in the production of the microorganism culture of the present invention is an energy source, a carbon source, a nitrogen source, an inorganic salt, a growth factor and the like suitable for culturing gastric microorganisms . &Lt; / RTI &gt; Energy sources, carbon sources, nitrogen sources, inorganic salts, and growth factors suitable for culturing gastric microorganisms are known in the art. Specifically, Korean Patent Application No. 19950031490, Korean Patent Application No. 1019910018012, Korean Patent Reference can be made to those disclosed in Korean Patent Application No. 1019940001029, Korean Patent Application No. 019990050363, Korean Patent Application No. 1020060049936, Jae-Bum Kim (Jae-Beom Kim, Cultivation of High Concentration Cell Oil of High-Nucleate Yeast Using Industrial Medium, 2001, . Such a medium may be a natural medium using natural materials such as milk, potato, blood, tomato crushed liquid, rice bran powder, soybean milk, molasses, or the like, or may be a synthetic medium artificially formed of nutrients, and may be a solid medium or a liquid medium. Preferably, natural medium is used for mass culture and economical aspect, and also liquid medium is used.

On the other hand, the culture conditions may be anaerobic or aerobic, and may be cultured or cultured. Both photosynthetic bacteria, lactic acid bacteria and yeast can be cultured in both anaerobic and aerobic conditions. It is known in the art that photosynthetic bacteria are heterotrophic in conditions of cancer culture. The incubation temperature is in the range of 20 ° C to 45 ° C, preferably in the range of 4.0 to 7.5, especially in the range of 6.0 to 7.5. However, anaerobic conditions may be preferable considering the growth characteristics of lactic acid bacteria.

In the production of the microorganism culture which is an effective ingredient of the present invention, the culture method may be a batch culture or a continuous culture.

The sterilization method of the medium may be any sterilization method known in the art. In particular, the high-pressure steam sterilization method and the ultraviolet sterilization method used in the embodiment of the present invention are suitable.

In the production of the microorganism culture which is an active ingredient of the present invention, it is preferable to add GABA (Gamma-amino butyric acid) to the culture medium and cultivate it.

GABA refers to a neurotransmitter produced from glutamate by glutamate decarboxylase, an amino acid present in the mammalian brain. In addition, GABA reduced blood pressure by activating blood vessel movement (Kayahara H. and Sgiura D., Food & Development., 36 (6): 4-6, 2001) (Ishikawa K. and Saito S., Psychopharmacology, 56: 127-131, 1978; Akaga T., Food & development, 36 (6): 7-9, 2001).

The addition of GABA has the effect of maintaining the microbial conjugate for a long period of time and increasing the size of the microbial conjugate as compared to the case where the microbial conjugate is not added, and since the microbial conjugate is an artificial one not formed in a natural state, And tend to return to the natural state (independent state, not combined state) by mutual action. The present inventors believe that the reason why the addition of GABA has the above effect is that the mutual stress between the microorganisms in the conjugate is reduced and the maintenance of the conjugate is continued.

When the microbial conjugate is increased by adding GABA, as shown in the following experimental example, the activity of purifying the pollutant increases.

The addition of GABA may be carried out at the time of preparing the medium or after the culture. When culturing is performed, it is preferable to inoculate photosynthetic bacteria, lactic acid bacteria, and / or yeast into the medium and start culturing, and then add them for about 96 hours. The added amount of Gabba may be in the range of 0.1 to 1.5 parts by weight based on 100 parts by weight of the medium.

Although not shown in the following Examples and Experimental Examples, the microbial conjugate contained in the culture of the microorganism as an active ingredient of the composition of the present invention, more precisely, the microbial conjugate contained in the culture, It is not particularly low compared to. Therefore, even if the composition of the present invention is injected into an ozone depleted in oxygen, the ozone depleting effect can be more effectively achieved.

In another aspect, the present invention relates to a method for purifying a waste matter using the composition for cleaning a waste matter of the present invention as described above.

The method for purifying a purifying agent of the present invention is characterized by comprising the steps of preparing the purifying agent for purifying the purifying agent of the present invention as described above and contacting the purifying agent for purifying the purifying agent with the purifying agent.

In the method of the present invention, the contacting step may be carried out by spraying, mixing, injecting, etc. the composition for cleaning a waste matter.

In addition, in order to improve the cleaning effect of the cleaning composition of the cleaning composition before and after the composition for cleaning the cleaning composition is brought into contact with the cleaning composition (that is, to improve the cleaning effect of the cleaning composition of the microorganism in the culture) An energy source, a carbon source, a nitrogen source, an inorganic salt, a growth factor, and the like, which are useful for the growth and culture of microorganisms, may be added together with the composition for purification. These ingredients may be artificially formulated or may be natural products such as milk, potato, blood, tomato crushed liquid, rice bran crushed, soybean milk, molasses and the like, and may be a mixture of an artificial composition and natural products.

In the method of the present invention, in addition to the use of the composition for purifying a purifying agent, a known substance / composition having a purifying function for purifying the purifying agent may be used together to enhance the purifying effect of the purifying agent.

In addition to the powder, calcium ascorbate, dried coral algae, red mud, loess and / or silica disclosed in Korean Patent Publication No. 2002-0023823 as such a substance / composition, a pH adjusting agent (magnesium compound disclosed in Korean Patent Publication No. 1997-0059106 , Compositions disclosed in U.S. Patent No. 5750484, etc.), powder coated with a natural polymer material disclosed in Korean Patent Publication No. 2004-0085856, a remover for heavy metals or toxic substances (a composition disclosed in Korean Patent Publication No. 2000-0063595, A composition disclosed in Korean Patent Publication No. 2000-0014117, a composition disclosed in Korean Patent Publication No. 1999-0080537, and the like), a suspending agent (a composition disclosed in U.S. Patent No. 6,039,875), and the like.

As described above, according to the present invention, it is possible to provide a method for purifying a waste material using the composition for purifying a waste material and the composition. The compositions and methods of the present invention can be found in natural, biological methods using lactic acid bacteria, photosynthetic bacteria and / or yeast combined cultures.

Fig. 1 shows the results obtained from the Lactobacillus plantarum and photosynthetic bacterium Rhodopsuedomonas palustris .
Fig. 2 is a graph showing the results obtained from the Lactobacillus acidophilus and photosynthetic bacterium Rhodopsedomonas palustris .
Fig. 3 is a graph showing the activity acidophilus , yeast Saccaromyces cerevisiae and photosynthetic bacteria Rhodopseudomonas micrograph of the palustris complex.
Fig. 4 is a graph showing the results obtained from the Lactobacillus acidophilus , yeast Saccaromyces cerevisiae and photosynthetic bacteria Rhodopseudomonas It is a photomicrograph of a palustris macroconjugate .

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited by these Examples and Experimental Examples.

< Example > Abscission  Preparation of Lactic Acid Bacteria, Photosynthetic Bacteria and / or Yeast Bodies Culture Liquid to be Used as a Purification Microorganism Preparation

&Lt; Example 1 > Lactobacillus plantarum and photosynthetic bacteria culture producing a conjugate of Rhodopseudomonas palustris

In order to prepare a combined product of the lactic acid bacteria and the photosynthetic bacteria, a culture medium capable of culturing lactic acid bacteria and photosynthetic bacteria together was prepared. The culture medium was 95 liters of water, 0.05 kg of crab shell crushed water, (0.03 kg), 0.02 kg of ground beef, 0.02 kg of ground beef, 0.02 kg of ground beef, 0.02 kg of ground beef, 0.02 kg of ground beef, 3.24 L of molasses, 1.5 L of milk and 0.5 kg of collagen powder were mixed uniformly.

Next, the prepared culture medium was sterilized at a sterilization temperature of 121 ° C for about 20 minutes. Then, the culture medium was placed in a fermentation container having a capacity of about 15 L, and the culture medium for the photosynthetic bacteria and the lactic acid bacteria culture were all 5% (v / v) And incubated at a culture temperature of 30 ° C, pH 6.5, and anaerobic conditions.

Approximately 16 days after the start of the culture, the culture broth was separated and the cultured microorganisms were photographed by an electron microscope.

Three photographs of the electron microscope are shown in Fig. Referring to the progress of FIG. 1, it can be seen that the lactic acid bacteria and the photosynthetic bacteria are clumped together to form a binding relationship. In Fig. 1, the white or small dotted shape is a photosynthetic bacterium, and the thin, angular rod shape is lactic acid bacteria.

&Lt; Example 2 > Lactobacillus acidophilus And culture of the conjugate of photosynthetic bacterium Rhodopsedomonas palustris

The same medium as in Example 1 (collagen was in the same amount as in Example 1), a fermentation vessel of the same capacity, and the like were used, and the culture medium of seed was inoculated in the same volume and the same fermentation conditions The lactic acid bacteria and the photosynthetic bacteria were cultured for about 16 days to obtain a combination of the lactic acid bacteria and the photosynthetic bacteria.

The results are shown in Fig. 2, and in Fig. 2, the long and thick rod shape is lactic acid bacterium, and the white spot small shape is photosynthetic bacteria.

&Lt; Example 3 > Lactobacillus acidophilus , yeast Saccaromyces cerevisiae and photosynthetic bacteria Rhodopseudomonas Preparation of culture media of palustris conjugates

The same medium as in Example 1 (containing collagen in the same amount as in Example 1), a fermentation vessel of the same capacity, and the like were used, and seed culture medium was inoculated in the same volume and the same fermentation conditions The lactic acid bacteria, the yeast and the photosynthetic bacteria were cultured for about 16 days to obtain a combination of the lactic acid bacteria, yeast, and photosynthetic bacteria.

The resultant conjugate can be identified in FIG. 3, wherein the long and thick rod shape is lactic acid bacteria, the elliptical shape is yeast, and the arrest or white small dot shape is photosynthetic bacteria.

<Example 4> lactic acid bacteria Lactobacillus plantarum , yeast Saccaromyces cerevisiae and photosynthetic bacteria Rhodopseudomonas Preparation of culture media of palustris conjugates

The same medium as in Example 1 (containing collagen in the same amount as in Example 1), fermentation vessels of the same capacity, and the like were inoculated with the same amount of the seed culture medium, and the same fermentation conditions The lactic acid bacteria, the yeast and the photosynthetic bacteria were cultured for about 16 days to obtain a combination of the lactic acid bacteria, yeast, and photosynthetic bacteria. However, 4 days after the initiation of culture, 0.3 kg of GABA (Gamma Amino Butyric Acid) was added and cultured.

The results are shown in Fig. 4, where a giant microbial conjugate was formed by the addition of Gabba. In Fig. 4, the long and thick rod shape is lactic acid bacteria, the elliptical shape is yeast, and the arrest or white small dot shape is photosynthetic bacteria.

< Comparative Example > Production of lactic acid bacteria, photosynthetic bacteria and / or yeast cultures

&Lt; Comparative Example 1 > Lactobacillus Preparation of cultures of photosynthetic bacterium Rhodopseudomonas palustris and plantarum

A culture solution of the lactic acid bacteria and photosynthetic bacteria was prepared in the same manner as in <Example 1>, except that no collagen was used. That is, the same medium, fermentation vessel of the same capacity, and the like were inoculated with the seed culture broth at the same dose, and the same lactic acid bacteria and the photosynthetic bacteria were cultured for about 16 days under the same fermentation condition to obtain a microorganism culture broth.

&Lt; Comparative Example 2 > Lactobacillus acidophilus And culture of photosynthetic bacterium Rhodopsedomonas palustris

A culture solution of the lactic acid bacteria and photosynthetic bacteria was prepared in the same manner as in <Example 2>, except that collagen was not used. That is, the same medium, fermentation vessel of the same capacity, and the like were inoculated with the seed culture broth at the same dose, and the same lactic acid bacteria and the photosynthetic bacteria were cultured for about 16 days under the same fermentation condition to obtain a microorganism culture broth.

&Lt; Comparative Example 3 > Lactobacillus acidophilus , yeast Saccaromyces cerevisiae and photosynthetic bacteria Rhodopseudomonas Preparation of cultures of palustris

A culture solution of the lactic acid bacteria, photosynthetic bacteria and yeast was prepared in the same manner as in Example 3, except that no collagen was used. That is, the same medium, fermentation vessel of the same capacity, and the like were inoculated with the seed culture broth at the same dose, and the lactic acid bacteria, the photosynthetic bacteria and the yeast were cultured for about 16 days under the same fermentation condition to obtain a microorganism culture broth.

< Manufacturing example > Using microbial culture Of the shaped body  Produce

50 parts by weight of microbial nutrients (mixture of an energy source, a carbon source, a nitrogen source, inorganic salts and the like) based on 100 parts by weight of loess, 100 parts by weight of loess, 100 parts by weight of loess, And 15 parts by weight of each microorganism culture solution of the comparative example were mixed, and the mixture was put into a molding mold and molded into a block having dimensions of length x length x height 2 x 4 x 2. The mixture was aged at a temperature of 37 캜 for 5 days after the molding, so that the microorganism was sufficiently fermented in the block-shaped body to finally produce a molded body for purification of water quality.

< Experimental Example > Experiment of water purification activity

< Experimental Example  1> Experimental study on water purification effect of stream water using microorganism culture stock solution

The experiment was performed by placing 1.5 liters of river water in the Yangtze River in a 2 L capacity container, adding 2% (v / v) of the culture solution of the microorganism in the above Example or Comparative Example and stirring And then reacted for 24 hours.

The values of COD (chemical oxygen demand), BOD (biological oxygen demand), total nitrogen (T-N) and total phosphorus (T-P) before and after the reaction were measured and shown in Table 1 below.

Stream water COD, BOD, T-N and T-P before and after reaction division COD (ppm) BOD (ppm) T-N (ppm) T-P (ppm) Stream before the reaction 26.7 34.3 13.48 1.24 After the treatment of the culture medium of Example 1 21.3 27.6 11.87 1.13 After the treatment of the culture medium of Example 2 22.1 26.9 11.58 1.15 After the treatment of the culture medium of Example 3 20.8 25.4 11.14 1.14 After the treatment of the culture medium of Example 4 17.8 23.3 9.96 1.02 After the treatment of the culture medium of Comparative Example 1 25.5 32.7 13.16 1.21 After the treatment of the culture medium of Comparative Example 2 25.7 33.2 12.99 1.19 After the treatment of the culture medium of Comparative Example 3 24.6 31.7 12.76 1.16

The results of the above Table 1 show that the microbial culture solution obtained in the above Examples has a much higher water purification effect than the microbial culture solution of the Comparative Example. Particularly, when the culture medium of the macroconjug obtained in Example 4 was used, the water purification effect was the most excellent.

< Experimental Example  2> Experimental Study on Removal of Odor from Livestock Wastewater Using Microorganism Culture Solution

1.5 liters of livestock wastewater from a livestock farming household in Gyeonggi-do were placed in a 2 liter capacity container, 2% (v / v) of each microorganism culture solution of the above example or comparative example was added and stirred, Lt; / RTI &gt;

The concentrations of ammonia, methyl mercaptan, hydrogen sulfide, and trimethylamine, which cause odor before and after the reaction, were measured and the removal rate was calculated according to the following equation, and is shown in Table 2 below.

 (%) = [(Concentration of gas before reaction (ppm) - concentration of gas after reaction (ppm)) / (concentration of gas before reaction (ppm))] 100

Removal rate (%) of ammonia, methyl mercaptan, hydrogen sulfide and trimethylamine division ammonia Methyl mercaptan Hydrogen sulfide Trimethylamine After the treatment of the culture medium of Example 1 87.3 96.7 91.3 93.8 After the treatment of the culture medium of Example 2 87.5 96.2 92.7 94.7 After the treatment of the culture medium of Example 3 91.3 98.7 94.8 96.4 After the treatment of the culture medium of Example 4 98.7 100 99.6 99.9 After the treatment of the culture medium of Comparative Example 1 67.8 93.3 86.9 82.3 After the treatment of the culture medium of Comparative Example 2 64.9 92.6 87.1 83.6 After the treatment of the culture medium of Comparative Example 3 74.5 95.7 90.5 89.3

The results of the above Table 2 show that the microorganism culture solution of the Example exhibits a high elimination activity against ammonia, methyl mercaptan, hydrogen sulfide and trimethylamine which cause odor. The removal activity of the microbial culture solution of this example was significantly higher than the removal activity of the microbial culture solution of the comparative example, and particularly the use of the macrobial culture solution obtained in Example 4 showed the highest removal activity.

< Experimental Example  3> Heavy metals in livestock wastewater using microorganism culture stock solution Abatement  Effect experiment

1.5 liters of livestock wastewater from a livestock farming household in Gyeonggi-do were placed in a 2 liter capacity container, 2% (v / v) of each microorganism culture solution of the above example or comparative example was added and stirred, Lt; / RTI &gt;

The concentration of harmful heavy metals such as cadmium, nickel, chromium and zinc was measured before and after the reaction, and the reduction rate was calculated according to the following formula, and is shown in Table 3 below.

(%) = [(Concentration of harmful heavy metal before reaction (ppm) - concentration of harmful heavy metal after reaction (ppm)) / (concentration of harmful heavy metal before reaction (ppm))] 100

% Reduction of cadmium, nickel, chromium and zinc division cadmium nickel chrome zinc After the treatment of the culture medium of Example 1 58.7 59.3 48.7 69.3 After the treatment of the culture medium of Example 2 55.3 56.2 45.3 67.5 After the treatment of the culture medium of Example 3 63.2 64.2 49.6 72.4 After the treatment of the culture medium of Example 4 72.5 75.6 55.2 78.6 After the treatment of the culture medium of Comparative Example 1 42.3 39.2 25.7 52.7 After the treatment of the culture medium of Comparative Example 2 39.2 37.9 27.6 51.4 After the treatment of the culture medium of Comparative Example 3 45.9 42.4 27.8 54.9

The results of the above Table 3 are also similar to the results of Table 1 and Table 2, and the heavy metal reduction rate of the microbial cultures of the Examples is much higher than the heavy metal reduction rate of the microbial cultures of the Comparative Examples Here, the use of the culture medium of the macroconjugate of Example 4 was the most harmful heavy metal reducing activity.

< Experimental Example  4> Microbial culture solution The molded article  Experimental effect of water purification

In the experiment of the purification of water quality using the molded product of the manufacturing example, the river water was used in the same way as the <Experimental Example 1>, and 3 liters of river water of the Yangcheng Stream was put into a container of 5 liter capacity, 10 block molds were put in and reacted for 2 weeks.

The values of COD (chemical oxygen demand), BOD (biological oxygen demand), total nitrogen (T-N) and total phosphorus (T-P) before and after the reaction were measured and shown in Table 4 below.

Stream water COD, BOD, T-N and T-P before and after reaction division COD (ppm) BOD (ppm) T-N (ppm) T-P (ppm) Stream before the reaction 26.7 34.3 13.48 1.24 After the treatment of the culture medium of Example 1 14.6 17.2 10.23 0.98 After the treatment of the culture medium of Example 2 15.2 16.4 10.37 0.96 After the treatment of the culture medium of Example 3 12.1 14.2 9.82 0.86 After the treatment of the culture medium of Example 4 8.7 10.7 8.37 0.78 After the treatment of the culture medium of Comparative Example 1 17.4 24.2 11.29 1.11 After the treatment of the culture medium of Comparative Example 2 18.3 23.6 11.43 1.03 After the treatment of the culture medium of Comparative Example 3 17.5 21.5 10.12 9.84

The results of the above Table 4 were obtained by using the block-shaped bodies prepared by adding the microorganism culture solutions of the above-mentioned Examples or Comparative Examples, and the results are shown in Table 4. In comparison with the results of Table 1 of the above Experimental Example 1, This is the result obtained by varying the time. The results of the above Table 3 are generally similar to those of the above Table 1, suggesting that a similar water purification effect can be obtained even if a microorganism having a water purification activity is produced as a molded body. Here, the effect of purifying the water was most excellent when the culture medium of the macroconjug obtained in Example 4 was used.

Claims (6)

(a) preparing a culture medium for culturing two or more microorganisms of photosynthetic bacteria, lactic acid bacteria and yeast,
(b) inoculating and culture two or more microorganisms of photosynthetic bacteria, lactic acid bacteria and yeast in said medium, and
(c) a microbial culture obtained by the method for producing a microbial culture comprising the step of adding collagen particles during the preparation of the medium of step (a) or during the culture of step (b) as an active ingredient,
Wherein the gauze is added at the time of preparing the medium of step (a) or during the culturing step of step (c).
The method according to claim 1,
The photosynthetic bacteria include Rhodopseudomonas bacteria sp . )ego,
The lactic acid bacteria are lactic acid bacteria of the genus Lactobacillus sp . ),
Wherein the yeast is a yeast of Saccharomyces spp.
The method according to claim 1,
Wherein the collagen is added in an amount of 0.3 to 2.0 parts by weight based on 100 parts by weight of the culture medium.
delete The method according to claim 1,
Wherein the GABA is added in an amount of 0.1 to 1.5 parts by weight based on 100 parts by weight of the medium.
A method for purifying a waste matter comprising the steps of: preparing the composition for cleaning a waste matter according to any one of claims 1 to 5; and contacting the composition for cleaning the waste matter with a waste material.

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