WO2017150742A1 - Coastal surface sediment remediation agent - Google Patents

Abstract

The present invention relates to a sediment remediation agent for eco-friendly cleaning up of coastal surface sediments. According to the present invention, the sediment remediation agent is formed by mixing an oxygen generator capable of generating oxygen at a surface layer and bio-balls supporting microorganisms in zeolite. The sediment remediation agent was sprayed on the surface of the coast and exhibited excellent effects in various areas such as chemical oxygen demand, total nitrogen and total phosphorus. It is expected that an ecological environment of a coast can be changed in an eco-friendly manner by spraying the sediment remediation agent of the present invention on the surface of the coast and simultaneously introducing a technique for resolving the oxygen-deficient water mass layer of the coast.

Description

Coastal Surface Sediment Enhancers

The present invention relates to a coastal quality improvement agent for improving the environmental pollution caused by sediment deposited on the bottom of the coast, rivers, lakes.

The coastal surface, together with the aquatic layer, constitutes the aquatic ecosystem and provides habitat for benthic creatures and is closely linked to the aquatic environment. Pollution in coastal waters leads to water pollution and adversely affects the vegetation of species sensitive to environmental changes.

An important factor in coastal surface contamination is coastal sedimentation.

Coastal sediments are formed as sediment enters the sea, including living sewage, concentrated livestock sewage, farm dung and sewage. Pollution gradually intensifies as the inflow and deposition of contaminants exceeds the natural self-cleaning capacity. Especially in the case of coasts with semi-closed or closed coastlines, such as the south coast of Korea, seawater exchange is not smooth and the degree of pollution deepens very quickly. In other words, microorganisms in the coastal surface decompose coastal deposits (organic matter) while consuming oxygen. However, due to the difference in density between the water layers of the seawater, the mixing between the layers is not performed, so that the oxygen supply is not smooth. The lack of oxygen reduces the activity of benthic organisms in the sediments, and the production of phytoplankton, phosphorus, nitrogen, which produces red algae, green algae, blue algae, and hypoxic water masses.

Degradation of organic matter in anaerobic sediments can also produce toxic by-products such as hydrogen sulfide or ammonia, limiting the number and type of species inhabiting the sediment.

Conventionally, methods such as aeration and dredging are performed to improve contaminated surface sediments, but these methods have problems such as spreading of water pollutants, stopping fish farming, treating secondary pollutants, and excessive execution cost. .

In addition, there is a method of spraying drugs such as clay and quicklime as another method for treating sediments, but clay spraying has a problem in that alumina and silica components in clay aggregate and precipitate organic suspensions in water. In addition, the calcium component in quicklime reacts with sulfate in water to form limestone and gypsum to cover the deposit, thus blocking the oxygen contact of living organisms in the deposit and causing a lot of damage.

In addition, the method of improving the environment of the sediment layer by using the base substitution ability and pH control effect of these materials by spraying ocher, zeolite, etc., but they are used to improve the low quality by simply adsorbing microorganisms that decompose organic matter. The stabilization of degrading microorganisms does not achieve a clear effect. Recently, improvements using biological methods have been attempted.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems, and an object thereof is to provide an improving agent including a biological agent to improve the contaminated coastal surface deposits.

That is, it provides a remediator for removing surface organic matter (LOM) contaminants that affect oxygen consumption. In particular, it is intended to provide an improved material that decomposes marine source organic matter when oxygen is deficient due to poor oxygen in summer. Most organic matter in surface deposits is labile oganic matter, so oxygen consumption can be reduced by supplying a modifier.

Coastal surface sediment improver according to the present invention for achieving the above object is, for cleaning the contaminated sediment is sprayed on the contaminated sediment of the coastal surface, the oxygen generator for supplying oxygen to the coastal surface sediment; And a bio ball fixed with microorganisms for removing organic matter and contaminants in the deposit using a porous crystalline aluminosilicate as a carrier, wherein the microorganisms include phototrophic bacteria, lactobacillus bacteria, and Bacillus. It is characterized by the inclusion of (bacillus) bacteria and pseudomonas bacteria.

Sediment improver according to the present invention has an excellent effect in a variety of environmental items, such as chemical oxygen demand, total nitrogen, total phosphorus of the coastal surface layer. In particular, when the bioball is more than two times, more preferably three times more than the oxygen supply agent, it exerts an excellent effect in all items including ecotoxicity.

The sediment improver according to the present invention is expected to be able to change the ecological environment of the coast by introducing a technology for dissolving the vacant oxygen lumps in the coast at the same time.

On the other hand, even if the effects are not explicitly mentioned herein, the effects described in the following specification expected by the technical features of the present invention and its provisional effects are treated as described in the specification of the present invention.

Coastal surface sediment improver according to the present invention for achieving the above object is, for cleaning the contaminated sediment is sprayed on the contaminated sediment of the coastal surface, the oxygen generator for supplying oxygen to the coastal surface sediment; And a bio ball fixed with microorganisms for removing organic matter and contaminants in the deposit using a porous crystalline aluminosilicate as a carrier, wherein the microorganisms include phototrophic bacteria, lactobacillus bacteria, and Bacillus. It is characterized by the inclusion of (bacillus) bacteria and pseudomonas bacteria.

According to the invention, lipolytic yeast and pseudomonas bacteria and nitrosomonas bacteria can additionally be immobilized on the carrier.

In addition, according to an embodiment of the present invention, it is preferable that the nitrobacter bacteria are additionally fixed to the carrier.

In one embodiment of the present invention, the oxygen generating agent may be used in the form of any one of hydroxides, oxides and peroxides of magnesium and calcium.

In one embodiment of the present invention, with respect to 100 parts by weight of the oxygen generating agent, the bio ball is preferably blended in a ratio of 200 to 350 parts by weight.

In the following description of the present invention, when it is determined that the subject matter of the present invention may be unnecessarily obscured by the person skilled in the art with respect to the related well-known functions, the detailed description will be omitted.

The present invention relates to sediment improvers for environmentally purifying coastal surface sediments. However, the present invention is applicable not only to sediments of coastal surface layers, but also to contaminated sediments of lakes and rivers. In addition, the pollutant of the sediment to be treated in the present invention is mainly an organic substance, but also includes all inorganic substances such as nitrogen and phosphorus.

Hereinafter, the coastal surface sediment improver (hereinafter referred to as 'sediment improver') according to an embodiment of the present invention will be described in more detail.

Sediment improver according to an embodiment of the present invention is made by mixing the oxygen generating agent and the biological agent.

Coastal surface sediment contaminants are primarily organic. This is due to the fact that various organic matters generated by the concentration of the population in a certain area and the concentrated concentration of the agricultural industry are introduced into the coast along with wastewater and sewage. If organic matter is introduced more than natural purification capacity, they cannot be decomposed and cause eutrophication. Organic matter is decomposed mainly by aerobic microorganisms. As oxygen is deficient due to eutrophication, organic matter is accumulated and accumulated continuously. If this condition persists over the long term, the sediment will turn into an anaerobic environment and the pollution will become more severe.

In the present invention, the improvement agent is formed by mixing the microorganism as the oxygen generating agent and the biological agent, as described above. In other words, it is intended to decompose contaminants by supplying oxygen and microorganisms together.

Oxygen generators in the present invention are used in the form of oxides, peroxides and hydroxides of divalent cations such as calcium and magnesium. For example, in the present embodiment, at least one or two or more of MgO, CaO, MgO ₂ , CaO ₂ , Mg (OH) ₂ , and Ca (OH) ₂ are used in combination. In particular, the peroxide form is mainly used.

Peroxides, MgO ₂ and CaO ₂ , react with water to convert oxygen into magnesium hydroxide and calcium hydroxide to release oxygen.

MgO ₂ + H ₂ O = Mg (OH) ₂ + 1/2 O ₂

CaO ₂ + H ₂ O = Ca (OH) ₂ + 1/2 O ₂

Since the above reaction occurs continuously over a long time, it is possible to smoothly supply oxygen to the deposit. When oxygen is supplied, it creates an atmosphere in which aerobic microorganisms can act.

In the present invention, the bio ball is a form in which microorganisms are adsorbed and immobilized on a carrier.

Porous, crystalline materials are used as the carrier, and zeolite is particularly used in this embodiment. Zeolites can be used as adsorbents of contaminants as porous materials. This is because zeolite has a high C.F.C (Cation Exchange Capacity). In addition, since zeolite is porous and has a large specific surface area where reaction can occur, it has an advantage of functioning as a very good adsorbent of pollutants. Above all, since the carrier must be able to immobilize a large number of microorganisms, it is advantageous to use a zeolite which is a porous material having a large specific surface area.

In summary, the zeolite in the present invention performs the function of the adsorbent which adsorbs and removes contaminants in water or sediment by itself and as a carrier for microorganisms to be stably received.

In this embodiment, the zeolite is used by firing and has a particle size of approximately 1 to 3 mm. When the particle diameter is 1 to 3mm, it is possible to maximize the fixed amount of the microorganisms and the adsorption of contaminants.

Various kinds of microorganisms are immobilized on the carrier.

In the present invention, phototrophic bacteria, lactobacillus bacteria, bacillus bacteria, and pseudomonas bacteria are immobilized on a carrier. As a method of fixing the microorganism to the carrier, various known methods can be used. That is, the microorganisms may be immobilized by culturing in a carrier, and the pre-cultured microorganisms may be adsorbed onto the carrier by a spray method using an adsorbent such as sodium alginate.

Phototropic bacteria is a generic term for bacteria that perform carbon assimilation using light energy. Purple and green bacteria can be used, and may contain halobacteria. More specifically, rhodopila, rhodobacter, chlorobium, or the like may be used. Red bacteria and green bacteria fix nitrogen and decompose organic matter. Degradation of organics lowers the chemical oxygen demand (COD) and turbidity in the deposits.

Lactobacillus bacteria produce lactic acid mainly from glucose and are gram-positive rod-like bacteria. More specifically, Lactobacillus casei, L. bulgaricus, L. plantarum, L. acidophilus, L. delbruckii, L. brevis, L. fermenti, L. bifidus and the like may be used. Lactobacillus bacteria also break down sediments and organics in the water column.

Bacillus bacteria are rod-shaped or cylindrical bacteria, which have various shapes such as long and short, but their size and shape are almost constant according to species. Bacillus bacteria are divided into positive and negative bacteria by gram staining. More specifically, in this embodiment, Bacillaceae, Lactobacillaceae, and the like may be used as Gram-positive Bacillus bacteria, and Enterobacteriaceae, Pseudomo-nadaceae, etc. may be used as Gram-negative Bacillus. Bacillus is a microorganism with a multiple enzyme system that can decompose proteins, carbohydrates, and lipids, and can remove nitrogen and lead.

Pseudomonas bacteria are Gram-negative bacilli that are very widely distributed in soil and water, and are generally mobile and have one or several polar flagella. Many pigments (piocyanine, fluoresine) are produced, and the medium may be colored or fluoresce. Generally aerobic, but anaerobic growth in denitrification or nitric acid breathing. It is widely used to decompose organic substances such as aliphatic hydrocarbons, aromatic hydrocarbons, phenols, terpenes and steroids.

In the present invention, lipolytic yeast, nitrosomonas bacteria, and nitrobacter bacteria can be immobilized on a carrier.

In particular, the present invention has another feature of using bacteria in nitrosomonas and nitrobacter to remove ammonia.

Nitrosomonas bacteria are the first genus of nitrite bacteria, ellipsoidal or simple intermittent, with one to two subpolar flagella. It is a gram-negative, chemically independent nutrient that oxidizes ammonia to nitrous acid and simultaneously fixes carbonic acid. It is a combination aerobic bacteria and is distributed in freshwater, ocean, and soil. Specifically, N. europaea can be used. It can be removed by oxidizing ammonia in the deposit.

Nitrobacter bacteria are the first genus of nitrate bacteria, a type of simple bacilli, which proliferate into buds and are nonmotile gram-negative. Many strains are organized chemically independent nutrients that oxidize nitrous acid to nitric acid and fix it at the same time. A few strains grow heterotrophically, but growth is slower than when they grow autotrophically. Reproductive sites are soil, fresh water, ocean, etc. In this embodiment, N. winogradskyi is used.

Meanwhile, in the present invention, gram-negative Brachymonas denitrificans is used to achieve aerobic denitrification and sludge reduction. You can also use proteinclasticum ruminis, a proteolytic anaerobic bacterium. It is also possible to use bacteroidales capable of anaerobic denitrification.

In the present invention, the microorganism may further include sulfur bacteria. Sulfur bacteria are a group of bacteria that oxidize sulfur and its compounds to obtain energy among autorophic bacteria. More specifically, beggiatoa, thiothrix, thioploca, acromatium, acidthiobacillus, chromatium thiocapsa, chloro Chlorobium and the like may be used.

The microorganisms have a cell count of about 1 × 10 ⁸ to 1 × 10 ⁹ cfu / g. To do this, the number of cells per gram of culture should be larger than the above number.

As described above, the bioball and the oxygen generator in which microorganisms capable of decomposing and removing organic and inorganic substances are immobilized on a carrier may be used in a range of 200 to 350 parts by weight of a bioball based on 100 parts by weight of an oxygen generator. . The blending ratio is very important, and through various experiments to be described later, it is advantageous to mix the oxygen generator: bioball 1: 3 parts by weight. In terms of decomposition and removal of organic and inorganic matters, bioballs can achieve a sufficient effect of 1.5 to 2.5 compared to oxygen generators, but the ratio of 1: 3 is the best when considering ecotoxicological effects.

The researchers conducted various experiments on the removal of contaminants by varying the ratio of oxygen generator and bioball. And only each component of the sediment according to the present invention was separated and tested together. The experimental results will be described below.

(1) sample

In the experiment, six sediment improvers of A, D, E, F, G, and H were used, and a sample without any treatment was used as a control. The A improver for comparison with the present invention is a microbial agent mainly composed of Brachymonas denitrificans, proteinclasticum ruminis and Bacteroides bacterium, and the D improver is an oxygen generating agent, mainly composed of MgO ₂ and CaO ₂ , and the E improver is zeolite It is an improver consisting of only bioballs that contain microorganisms.

And sediment improver according to the present invention is F, G, H as F is a mixture of a bio ball and an oxygen generator in a 1: 1, G is a mixture of an oxygen generator and a bio ball in a 2: 1, H is Conversely, the oxygen generator and the bioball are combined at 1: 2. In addition, the bioball contained all of the microorganisms mentioned in the examples of the present invention, and each bacterium was tested to have a number of cells of 1 × 10 ⁸ to 1 × 10 ⁹ cfu / g.

(2) Experiment method

-Physicochemical Component Analysis

The sediment test was conducted in accordance with the Marine Environmental Process Test Method. The pH and oxidation reduction potential (ORP) of the sediments were measured five times for 28 days with a measuring instrument (ORION model 210A, USA). The pH and ORP were measured directly from the sediments of each reactor through the electrodes.

Chemical Oxygen Demand (COD) was measured by alkaline potassium permanganate method.The expression of sodium thiosulfate solution was placed in a 250 ml Erlenmeyer flask with 25 ml of 0.1 N sodium dichromate solution, and then 100 ml of distilled water and 10 ml of potassium iodide solution and 10% sulfuric acid solution. 2 ml of solution was added. The solution was allowed to cool and then titrated with 0.1 N sodium thiosulfate solution. The titer f after titration was calculated as follows.

f = 25 / (quantity entered)

AVS (Acid Volatile Sulfide) was carried out by the sulfur detection method, and 2 g of wet sediment was transferred to the gas generating pipe combined with the detection pipe, and then 2 ml of 18 N sulfuric acid (H2SO4) was added and pumped for a predetermined time. AVS was calculated by reading the scale when the discoloration of was stopped. At this time, the suction time was observed and the generated gas was not leaked. The yellow detection tube used in the experiment (Detectop Tube NO. 201H, GASTEC, Japan) was used.

T-N (Total Nitrogen) and T-P (Total Phosphorus) were collected by centrifugation of 20 g of wet sediments, and the supernatant was collected, filtered and diluted with GF / C (47 mm), followed by seawater method. TN was decomposed into alkaline potassium persulfate, oxidized to nitrate nitrogen, and passed through a cadmium-copper reduction column to reduce nitrite ions to nitrite ions. The colorimetric quantification was performed using a spectrophotometer UV-1800 (Shimadzu, USA) to wavelength 543 nm. Absorbance was measured at. T-P was oxidatively decomposed with potassium persulfate, changed to phosphate (PO4-P) form, and then colorimetrically determined by ascorbic acid reduction. Absorbance was measured at a wavelength of 885 nm with UV-1800 (Shimadzu, USA).

The moisture content of the sediment was dried in a constant amount, and then weighed in a predetermined amount in a weighed bottle, and then dried at 110 ° C. for 24 hours at a drying temperature. After the first drying, the weight was measured, dried again for 12 hours or more, and dried until the weight was measured, and the weight of the dried bottle was measured. The difference between the weight before drying and the weight after drying was measured.

Moisture content = (sample weight when drying-sample weight after drying) / sample weight before drying

Total Viable Counts (TVCs)

In the analysis, the sample is 100g of sediment of each reactor in a beaker, shaken for 30 minutes, the supernatant is collected, diluted 100-fold, 1 ml of the solution uniformly inoculated in Marine Agar medium, and then incubated for 2 days at room temperature, Colony counts were calculated on the colony count, multiplied by the dilution factor, and finally the result was obtained.

(3) Results and Discussion

A. Environmental Change Survey on Sediments of Individual Improvement Agents (A, D, E) and Complex Improvement Agents (F)

[Table 1] COD analysis of sediments of individual improvers (A, D, E) and complex improvers (F)

[Table 2] AVS analysis of sediments of individual improvers (A, D, E) and complex improvers (F)

[Table 3] T-N analysis of sediments of individual improvers (A, D, E) and complex improvers (F)

[Table 4] T-P analysis of sediments of individual improvers (A, D, E) and complex improvers (F)

[Table 5] Analysis of total bacterial counts on sediments of individual improvers (A, D, E) and complex improvers (F)

Referring to Tables 1 to 5 above, chemical oxygen demand (COD), AVS (acidic volatile sulfide), total nitrogen (TN), total phosphorus (TP) and total bacterial counts were tested. Although there were some matters in which the improver or the E improver had the most excellent effect, it was confirmed that the improver F according to the present invention showed an excellent effect evenly in all items.

The researchers tested the improver F on the sediment as well as on the water column. The result is as follows.

B. Environmental Changes in Water Layers of Individual Improvement Agents (A, D, E) and Complex Improvement Agents (F)

[Table 6] COD analysis of water layers of individual improvers (A, D, E) and complex improvers (F)

[Table 7] T-P analysis of the water layer of individual improvers (A, D, E) and complex improvers (F)

[Table 8] PO ₄ -P Analysis of Water Layers of Individual Enhancers (A, D, E) and Complex Enhancers (F)

[Table 9] T-N analysis of the water layer of individual improvers (A, D, E) and complex improvers (F)

[Table 10] NO ₂ -N Analysis of Water Layers of Individual Enhancers (A, D, E) and Complex Enhancers (F)

[Table 11] NO ₃ -N Analysis of Water Layers of Individual Improvement Agents (A, D, E) and Complex Improvement Agents (F)

[Table 12] NH ₄ -N Analysis of Water Layers of Individual Improvement Agents (A, D, E) and Complex Improvement Agents (F)

Referring to Tables 6 to 12 above, chemical oxygen demand, total phosphorus, phosphate phosphorus, total nitrogen, nitrite nitrogen, nitrate nitrogen, and ammonia nitrogen were tested. Although there were some matters with the most excellent effect, it was confirmed that the improver F according to the present invention showed excellent effects evenly in all items.

On the other hand, the researchers confirmed the superiority of the sediment improver mixed with the bio-ball containing the oxygen generator and the microorganism according to the present invention through the above experiment, more specifically, according to the mixing ratio of the oxygen generator and the bio-ball An experiment was conducted to confirm the difference in effects. The result is as follows.

C. Environmental Changes in Sediments According to Mixing Ratio

[Table 13] COD Analysis of Sediments According to Mixing Ratio

[Table 14] AVS Analysis of Sediments by Mixing Ratio

[Table 15] T-N Analysis of Sediments According to Mixing Ratio

[Table 16] T-P Analysis of Sediments According to Mixing Ratio

Referring to Tables 13-16 above, almost; In most cases, the effect was better when the bioball contained higher weight than oxygen generator. That is, when the oxygen generating agent is 2: 1 compared to the bioball, the environmental treatment of the sediment layer was excellent.

The researchers conducted the same experiments on the water column as well as the sediments. The result is as follows.

D. Investigation of Environmental Changes in Water Layer According to Mixing Ratio

[Table 17] COD Analysis of Water Layers by Mixing Ratio

[Table 18] T-P Analysis of Water Layer According to Mixing Ratio

[Table 19] PO ₄ -P Analysis of Water Layer According to Mixing Ratio

Table 20: T-N Analysis of Water Layers with Mix Ratios

[Table 21] NO ₂ -N Analysis of Water Layers with Mix Ratio

[Table 22] NO ₃ -N Analysis of Water Layers by Mixing Ratio

[Table 23] NH ₄ -N Analysis of Water Layer According to Mixing Ratio

Referring to Tables 17 to 23 above, the results of experiments on chemical oxygen demand, total phosphorus, phosphate phosphorus, total nitrogen, nitrite nitrogen, nitrate nitrogen, and ammonia nitrogen for the water layer were found. It was confirmed that the case included at twice the weight with respect to the agent showed a better effect.

In addition, the researchers performed an ecological impact assessment for the sediment improver according to the present invention. For this, two benthic star clusters were selected and tested. The benthic unipod Mandibulophoxus mai was collected on the sands of Manlipo and Cheonlipo , Taean-gun, Chungcheongnam-do prior to the test. acherusicum used test subjects in constant culture at NeoEnbiz Laboratory. M. acherusicum, which has been cultivated in the laboratory at all times, has been subcultured since 2003 when it was collected from the tidal flats of Daebu Island.

The seawater used for the experiment was supplied by filtered seawater from the Incheon Fisheries Research Institute located in Yeongheung-do, Incheon. The quality of the experimental seawater was always checked during the incubation period and throughout the experimental period. Basic water quality items were water temperature, salinity, pH, dissolved oxygen and ammonia. Except for temperature and salt tolerance experiments, water temperature was 20 ± 1 ℃, salinity was 30 ± 1 psu, pH was 8.0 ± 0.5, dissolved oxygen was> 80% and ammonia was <1 mg, regardless of species. L-1 was maintained. Experimental seawater was exchanged daily instead of aeration in the water-only test, and exchanged once every 5 days in the aeration of the overlying water in the sediment toxicity test.

Refer to the existing literature for a detailed description of the environmental factor tolerance and hazardous substance sensitivity testing and site sediment toxicity testing methods presented in this study (Lee et al., 2005a; Lee et al., 2005). The ecological impact test using shellfish reflects the effects of short-term exposure over 10 days, so it may be somewhat less sensitive than other chronic tests, but the test method is not only the most widely used in sediment toxicity testing worldwide. It is recognized as the most basic test method. This test method needs to be used more widely for the purpose of rapid screening for biological effects of sediment hazardous material contamination.

Sediment ecotoxicity assessment using benthic monopods has been widely used as a test method for assessing biological effects by comparing the survival rate or mortality in experimental and control groups after exposure to benthic monopods for 10 days. Refer to Annex 3. Waste Process Test Criteria).

The benthic monopod M. acherusicum, which is passaged in the laboratory, passes through the standard 500-μm mesh and passes the 300-μm standard, while the M. mai passes the 2 mm standard and the 1 mm standard does not Was selected for the test, and only healthy and active subjects were selected and used in the experiment without any external wounds or damage to appendages.

The sediments used for the test with the sediment improver were used to minimize the difference in sediment characteristics between the samples collected by mixing several times taken from the same peak. This homogenized sediment was passed through a 500-㎛ standard to remove coarse particles and endogenous organisms and used in the experiment.

After 10 days of exposure, 300-μm standard sieves were collected and the remaining subjects were collected and counted by observing surviving and deceased individuals under a microscope, and counting the survival rates (or mortality rates) using the counted results. Was calculated by the following equation.

S (%) = (Nf / N0) 100, M (%) = 100-S

[S: survival rate; Nf = number of final surviving individuals; N0: initial input population, M: mortality rate]

To determine whether the survival rate of each sample was statistically different from that of the control group, Student's t-test was conducted to determine the toxicity. The conditions of ecological impact test for sediment modifiers using benthic shellfish are shown in Table 24 below. Summarized in

TABLE 24

For the ecological impact assessment of sediment modifiers, two benthic monopods were selected and tested. Benthic Monopods Mandibulophoxus mai with Monocorophium acherusicum was used. Sediment modifiers for ecological impact assessments were tested for bioballs and oxygen supplies in the range of 1: 1 to 9: 1. And the experiment was divided into a method of spraying and mixing the sediment.

As a result of ecological impact assessment for the use of sediment improver, it was found that there is no ecological effect when spraying or mixing the surface of sediment when the composition of the bioball is 75% or more. (See Table 25).

TABLE 25

That is, it is preferable that the bioball is mixed by more than three times by weight of the bioball as compared with the oxygen supply agent. If you broaden the range, it should be included at least twice. And if more than three times more than the bio ball is not required, in summary, the bio-ball with 200 to 350 parts by weight of 100 parts by weight of oxygen supply, sediment and water layer environmental treatment and ecotoxicity It was confirmed that the best in terms of.

As described above, in the present invention, a bioball having microorganisms supported on zeolite and an oxygen supply agent composed of a peroxide of calcium and magnesium are mixed to use as a sediment improver for the coastal surface layer. Sediment improver according to the present invention was confirmed to have an excellent effect in a variety of environmental items such as chemical oxygen demand, total nitrogen, total phosphorus of the coastal surface layer. In particular, when the bioball is more than two times, more preferably three times more than the oxygen supply agent, it exerts an excellent effect in all items including ecotoxicity. In addition to using the sediment improving agent according to the present invention at the same time by introducing a technique for resolving the empty oxygen lumps in the coastal water layer will be able to environmentally change the ecological environment of the coast.

Claims (8)

1. It is intended to purify contaminated sediments by spraying them on the coastal surface.

   Oxygen generators for supplying oxygen to coastal surface sediments; And

   And a bio ball fixed with microorganisms for removing organic substances and contaminants in the deposit using a porous crystalline material as a carrier.

   The microorganism is a coastal surface sediment improver comprising a phototrophic bacteria, lactobacillus bacteria, bacillus bacteria and pseudomonas bacteria.
2. The method of claim 1,

   Lipolysis yeast (seeast) and pseudomonas (pseudomonas) bacteria to the carrier further characterized in that the surface sediment improver.
3. The method of claim 1,

   Nitrosomonas (nitrosomonas) bacterium surface sediment improver, characterized in that additionally fixed to the carrier.
4. The method of claim 1,

   A surface superficial sediment improver, characterized in that the nitrobacter bacteria are additionally immobilized on the carrier.
5. The method of claim 1,

   The microorganism is a coastal surface sediment improver comprising brachymonas denitrificans and bacteroidales bacteria.
6. The method of claim 1,

   The microorganisms include beggiatoa, thiothrix, thioploca, acromatium, acidthiobacillus, chromatium, thiocapsa, and Coastal surface sediment improver comprising all of the chlorobium (chlorobium).
7. The method of claim 1,

   The oxygen generator is a coastal surface sediment improver, characterized in that any one of the form of hydroxides, oxides and peroxides of magnesium and calcium.
8. The method of claim 1,

   With respect to 100 parts by weight of the oxygen generating agent, the bio-ball is a coastal surface sediment improver, characterized in that blended at a ratio of 200 to 350 parts by weight.