CN108865944A - A kind of microorganism live bacteria agent for fish raising - Google Patents
A kind of microorganism live bacteria agent for fish raising Download PDFInfo
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Abstract
The present invention provides a kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent B, compound microorganism bacterium powder agent A to be made of the component of following parts by weight：65-75 parts of A microbial inoculum, 8-12 parts of sodium alginate, 8-12 parts of polyvinyl alcohol, 5-10 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：20-30 parts of lactic acid bacteria, 6-12 parts of saccharomycete, 4-8 parts of photosynthetic bacteria, 10-18 parts of composite bacillus, 7-13 parts of actinomyces, 20-30 parts of cellulase, 15-28 parts of protease, 18-25 parts of lactobacillus plantarum；The compound microorganism bacterium powder agent B is made of the component of following parts by weight：65-75 parts of B microbial inoculum, 2-10 parts of microelement, 10-20 parts of diatomite, 5-10 parts of desiccant, wherein B microbial inoculum is mixed by the component of following parts by weight：8-15 parts of denitrification desulfurization bacterium, 8-15 parts of nitrifier, 15-25 parts of azotobacter chroococcum, 25-40 parts of zeolite powder, 15-20 parts of zytase.The microorganism live bacteria agent can effectively improve water quality, improve feeding fish living environment, reduce the death rate.
Description
Technical field
The present invention relates to aquaculture feeding fish fields, and in particular to a kind of microorganism live bacteria agent for fish raising.
Background technique
Fish be unable to do without water, and water is most basic most important element in fish culture.The water quality of " fertile, living, tender, refreshing " is that fish is supported
The basic guarantee for obtaining good harvest is grown, at home, is photosynthetic bacteria in aquaculture using more, is improving water quality and conduct
Good effect is obtained in terms of feed addictive, but strain is single, has not been able to satisfy wanting for a variety of cultivation objects and breeding environment
It asks, the demand of aquaculture should be adapted to using the multi-functional probiotics of more bacterial strains.At present both at home and abroad to fish culture disease
Harmful research is concentrated mainly on the type of disease, diagnosis, case, pathogenic bacteria pathogenic and in terms of the control of disease, and
Research in terms of health care, diseases prevention is relatively fewer.The work of the application methods of various microbial inoculums, Rational Dosage etc. there is still a need for do into
The research of one step, initiatively promotes and applies test, can be used for cultivating screening and the cell fixation institute of the microorganism of water process
Embedding medium type all will be the direction of fish culture microbe research and application from now on.
Summary of the invention
To solve the above problems, the present invention provides a kind of microorganism live bacteria agent for fish raising, the microorganism live bacteria agent
It can effectively improve water quality, improve feeding fish living environment, improve fish exterior quality, reduce the death rate.
The object of the present invention is achieved in the following manner：
A kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent
B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：65-75 parts of A microbial inoculum, gathers 8-12 parts of sodium alginate
8-12 parts of vinyl alcohol, 5-10 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：20-30 parts of lactic acid bacteria,
6-12 parts of saccharomycete, 4-8 parts of photosynthetic bacteria, 10-18 parts of composite bacillus, 7-13 parts of actinomyces, 20-30 parts of cellulase,
15-28 parts of protease, 18-25 parts of lactobacillus plantarum；The compound microorganism bacterium powder agent B is made of the component of following parts by weight：B
65-75 parts of microbial inoculum, 2-10 parts of microelement, 10-20 parts of diatomite, 5-10 parts of desiccant, wherein B microbial inoculum is by following parts by weight
Component is mixed：8-15 parts of denitrification desulfurization bacterium, 8-15 parts of nitrifier, 15-25 parts of azotobacter chroococcum, 25-40 parts of zeolite powder,
15-20 parts of zytase.
A kind of microorganism live bacteria agent for fish raising, the compound microorganism bacterium powder agent A are prepared by following methods：
(1) culture of microbial inoculum：It is 28-32 DEG C that temperature, which is arranged, by all components strain of A microbial inoculum in activation culture
Inoculation, activation culture 24-48h in base, the activation medium is by soy peptone 8g, glucose 20g, yeast powder 20g, water
1000ml is configured to culture solution, and pH is adjusted to neutrality, and cultured strain ferments in the fermentation medium then and expands numerous 24-
72h, the fermentation medium are peptone 5kg, yeast powder 0.8kg, glucose 15kg, magnesium sulfate 1kg, potassium dihydrogen phosphate
0.3kg, dipotassium hydrogen phosphate 0.3kg, ammonium sulfate 1kg, sodium citrate 0.05kg, iron chloride 0.05kg, cobalt chloride 0.02kg, sulfuric acid
Copper 0.03kg, zinc chloride 0.05kg, calcium chloride 0.05kg, vitaminB10 .05kg, vitamin B2 0.05kg, calcium pantothenate
0.03kg, folic acid 0.03kg, niacin 0.03kg are formulated, and are adjusted pH7.5, are settled to 1t with water.In activation culture and hair
Ferment expands numerous constantly shake or stirring in the process, and after obtaining bacterium solution, centrifugation is concentrated, is washed with distilled water thallus under room temperature, obtains A
Microbial inoculum；
(2) the resulting A microbial inoculum of step (1) is dried in the preparation of complex micro organism fungicide, and drying temperature is 40-50 DEG C, powder
Then the A bacterium powder is uniformly mixed, air drying by broken 120 mesh of mistake with the sodium alginate, polyvinyl alcohol, desiccant crushed,
Obtain compound microorganism bacterium powder agent A.
The compound microorganism bacterium powder agent B is prepared by following methods：
(1) culture of microbial inoculum：It is 30-35 DEG C that temperature, which is arranged, by all components strain of B microbial inoculum in activation culture
Inoculation, activation culture 24-48h in base, the activation medium is by soy peptone 8g, glucose 20g, yeast powder 20g, water
1000ml is configured to culture solution, and pH is adjusted to neutrality, and cultured strain ferments in the fermentation medium then and expands numerous 24-
72h, the fermentation medium are peptone 5kg, yeast powder 0.8kg, glucose 15kg, magnesium sulfate 1kg, potassium dihydrogen phosphate
0.3kg, dipotassium hydrogen phosphate 0.3kg, ammonium sulfate 1kg, sodium citrate 0.05kg, iron chloride 0.05kg, cobalt chloride 0.02kg, sulfuric acid
Copper 0.03kg, zinc chloride 0.05kg, calcium chloride 0.05kg, vitaminB10 .05kg, vitamin B2 0.05kg, calcium pantothenate
0.03kg, folic acid 0.03kg, niacin 0.03kg are formulated, and are adjusted pH7.5, are settled to 1t with water.In activation culture and hair
Ferment expands numerous constantly shake or stirring in the process, and after obtaining bacterium solution, centrifugation is concentrated, is washed with distilled water thallus under room temperature, obtains B
Microbial inoculum；
(2) the resulting B microbial inoculum of step (1) is dried in the preparation of complex micro organism fungicide, and drying temperature is 40-50 DEG C, powder
Then obtained B bacterium powder is uniformly mixed by broken 120 mesh of mistake with calcium carbonate, microelement, diatomite, the desiccant crushed,
Air drying obtains compound microorganism bacterium powder agent B.
The microelement is by zinc sulfate, magnesium sulfate and potassium dihydrogen phosphate=1：1：1 composition；The desiccant is swelling
One of soil, silica gel, white carbon black or attapulgite.
First by the activation medium and production fermentation medium respectively after 115-125 DEG C of high-temperature sterilization 30-50min,
It is cultivated strain is added.
A kind of application method of the microorganism live bacteria agent for fish raising, every liter of water amount of application is 0.1g, is first applied compound
Microbial bacteria pulvis A applies compound microorganism bacterium powder agent B again after 3-5 days.Water quality can be applied well once every 20-30 days；Water quality compared with
Difference should be applied primary every 10-15 days.
Compared with the existing technology, the microorganism live bacteria agent provided by the present invention for fish raising has the following advantages that：
1, ammonia nitrogen, hydrogen sulfide, nitrite and COD value is effectively reduced, stablizes pH value.
2, photosynthetic capacity is improved, water body color is improved, increases water transparency.
3, sustained release is supported, and oxygenation effect is more preferable.
4, profitable strain is activated, by the absolute predominance of microorganism beneficial bacterium, disintegrates rapidly and converts peculiar smell, stink, give fish
One excellent ecological environment of class improves fish exterior quality, reduces disease incidence.
Specific embodiment
Embodiment 1：
A kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent
B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：65-75 parts of A microbial inoculum, gathers 8-12 parts of sodium alginate
8-12 parts of vinyl alcohol, 5-10 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：20-30 parts of lactic acid bacteria,
6-12 parts of saccharomycete, 4-8 parts of photosynthetic bacteria, 10-18 parts of composite bacillus, 7-13 parts of actinomyces, 20-30 parts of cellulase,
15-28 parts of protease, 18-25 parts of lactobacillus plantarum；The compound microorganism bacterium powder agent B is made of the component of following parts by weight：B
65-75 parts of microbial inoculum, 2-10 parts of microelement, 10-20 parts of diatomite, 5-10 parts of desiccant, wherein B microbial inoculum is by following parts by weight
Component is mixed：8-15 parts of denitrification desulfurization bacterium, 8-15 parts of nitrifier, 15-25 parts of azotobacter chroococcum, 25-40 parts of zeolite powder,
15-20 parts of zytase.
A kind of microorganism live bacteria agent for fish raising, the compound microorganism bacterium powder agent A are prepared by following methods：
(1) culture of microbial inoculum：It is 28-32 DEG C that temperature, which is arranged, by all components strain of A microbial inoculum in activation culture
Inoculation, activation culture 24-48h in base, the activation medium is by soy peptone 8g, glucose 20g, yeast powder 20g, water
1000ml is configured to culture solution, and pH is adjusted to neutrality, and cultured strain ferments in the fermentation medium then and expands numerous 24-
72h, the fermentation medium are peptone 5kg, yeast powder 0.8kg, glucose 15kg, magnesium sulfate 1kg, potassium dihydrogen phosphate
0.3kg, dipotassium hydrogen phosphate 0.3kg, ammonium sulfate 1kg, sodium citrate 0.05kg, iron chloride 0.05kg, cobalt chloride 0.02kg, sulfuric acid
Copper 0.03kg, zinc chloride 0.05kg, calcium chloride 0.05kg, vitaminB10 .05kg, vitamin B2 0.05kg, calcium pantothenate
0.03kg, folic acid 0.03kg, niacin 0.03kg are formulated, and are adjusted pH7.5, are settled to 1t with water.In activation culture and hair
Ferment expands numerous constantly shake or stirring in the process, and after obtaining bacterium solution, centrifugation is concentrated, is washed with distilled water thallus under room temperature, obtains A
Microbial inoculum；
(2) the resulting A microbial inoculum of step (1) is dried in the preparation of complex micro organism fungicide, and drying temperature is 40-50 DEG C, powder
Then the A bacterium powder is uniformly mixed, air drying by broken 120 mesh of mistake with the sodium alginate, polyvinyl alcohol, desiccant crushed,
Obtain compound microorganism bacterium powder agent A.
The compound microorganism bacterium powder agent B is prepared by following methods：
(1) culture of microbial inoculum：It is 30-35 DEG C that temperature, which is arranged, by all components strain of B microbial inoculum in activation culture
Inoculation, activation culture 24-48h in base, the activation medium is by soy peptone 8g, glucose 20g, yeast powder 20g, water
1000ml is configured to culture solution, and pH is adjusted to neutrality, and cultured strain ferments in the fermentation medium then and expands numerous 24-
72h, the fermentation medium are peptone 5kg, yeast powder 0.8kg, glucose 15kg, magnesium sulfate 1kg, potassium dihydrogen phosphate
0.3kg, dipotassium hydrogen phosphate 0.3kg, ammonium sulfate 1kg, sodium citrate 0.05kg, iron chloride 0.05kg, cobalt chloride 0.02kg, sulfuric acid
Copper 0.03kg, zinc chloride 0.05kg, calcium chloride 0.05kg, vitaminB10 .05kg, vitamin B2 0.05kg, calcium pantothenate
0.03kg, folic acid 0.03kg, niacin 0.03kg are formulated, and are adjusted pH7.5, are settled to 1t with water.In activation culture and hair
Ferment expands numerous constantly shake or stirring in the process, and after obtaining bacterium solution, centrifugation is concentrated, is washed with distilled water thallus under room temperature, obtains B
Microbial inoculum；
(2) the resulting B microbial inoculum of step (1) is dried in the preparation of complex micro organism fungicide, and drying temperature is 40-50 DEG C, powder
Then obtained B bacterium powder is uniformly mixed by broken 120 mesh of mistake with calcium carbonate, microelement, diatomite, the desiccant crushed,
Air drying obtains compound microorganism bacterium powder agent B.
The microelement is by zinc sulfate, magnesium sulfate and potassium dihydrogen phosphate=1：1：1 composition；The desiccant is swelling
One of soil, silica gel, white carbon black or attapulgite.
First by the activation medium and production fermentation medium respectively after 115-125 DEG C of high-temperature sterilization 30-50min,
It is cultivated strain is added.
A kind of application method of the microorganism live bacteria agent for fish raising, every liter of water amount of application is 0.1g, is first applied compound
Microbial bacteria pulvis A applies compound microorganism bacterium powder agent B again after 3-5 days.Water quality can be applied well once every 20-30 days；Water quality compared with
Difference should be applied primary every 10-15 days.
Embodiment 2：
A kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent
B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：65 parts of A microbial inoculum, 12 parts of sodium alginate, polyvinyl alcohol
8 parts, 8 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：It is 20 parts of lactic acid bacteria, 10 parts of saccharomycete, photosynthetic
6 parts of bacterium, 12 parts of composite bacillus, 13 parts of actinomyces, 25 parts of cellulase, 15 parts of protease, 22 parts of lactobacillus plantarum；Institute
Compound microorganism bacterium powder agent B is stated to be made of the component of following parts by weight：70 parts of B microbial inoculum, 18 parts of diatomite, is done 5 parts of microelement
8 parts of drying prescription, wherein B microbial inoculum is mixed by the component of following parts by weight：10 parts of denitrification desulfurization bacterium, 15 parts of nitrifier, circle is brown
20 parts of nitrogen-fixing bacteria, 30 parts of zeolite powder, 15 parts of zytase.
The preparation method is the same as that of Example 1 by compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent B.
Embodiment 3
A kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent
B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：75 parts of A microbial inoculum, 10 parts of sodium alginate, polyvinyl alcohol
10 parts, 5 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：It is 25 parts of lactic acid bacteria, 12 parts of saccharomycete, photosynthetic
5 parts of bacterium, 16 parts of composite bacillus, 10 parts of actinomyces, 23 parts of cellulase, 20 parts of protease, 20 parts of lactobacillus plantarum；Institute
Compound microorganism bacterium powder agent B is stated to be made of the component of following parts by weight：68 parts of B microbial inoculum, 10 parts of diatomite, is done 2 parts of microelement
10 parts of drying prescription, wherein B microbial inoculum is mixed by the component of following parts by weight：8 parts of denitrification desulfurization bacterium, 8 parts of nitrifier, circle is brown solid
15 parts of nitrogen bacterium, 40 parts of zeolite powder, 20 parts of zytase.
The preparation method is the same as that of Example 1 by compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent B.
Embodiment 4
A kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent
B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：70 parts of A microbial inoculum, 8 parts of sodium alginate, polyvinyl alcohol 9
Part, 10 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：It is 30 parts of lactic acid bacteria, 6 parts of saccharomycete, photosynthetic thin
8 parts of bacterium, 10 parts of composite bacillus, 7 parts of actinomyces, 30 parts of cellulase, 28 parts of protease, 25 parts of lactobacillus plantarum；It is described
Compound microorganism bacterium powder agent B is made of the component of following parts by weight：72 parts of B microbial inoculum, 20 parts of diatomite, is done 10 parts of microelement
5 parts of drying prescription, wherein B microbial inoculum is mixed by the component of following parts by weight：15 parts of denitrification desulfurization bacterium, 10 parts of nitrifier, circle is brown
25 parts of nitrogen-fixing bacteria, 35 parts of zeolite powder, 18 parts of zytase.
The preparation method is the same as that of Example 1 by compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent B.
Embodiment 5
A kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent
B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：68 parts of A microbial inoculum, 9 parts of sodium alginate, polyvinyl alcohol
11 parts, 7 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：It is 28 parts of lactic acid bacteria, 8 parts of saccharomycete, photosynthetic
4 parts of bacterium, 14 parts of composite bacillus, 12 parts of actinomyces, 20 parts of cellulase, 24 parts of protease, 18 parts of lactobacillus plantarum；Institute
Compound microorganism bacterium powder agent B is stated to be made of the component of following parts by weight：65 parts of B microbial inoculum, 12 parts of diatomite, is done 8 parts of microelement
6 parts of drying prescription, wherein B microbial inoculum is mixed by the component of following parts by weight：12 parts of denitrification desulfurization bacterium, 12 parts of nitrifier, circle is brown
18 parts of nitrogen-fixing bacteria, 40 parts of zeolite powder, 15 parts of zytase.
The preparation method is the same as that of Example 1 by compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent B.
Embodiment 6
A kind of microorganism live bacteria agent for fish raising, including compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent
B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：72 parts of A microbial inoculum, 11 parts of sodium alginate, polyvinyl alcohol
8 parts, 6 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：It is 26 parts of lactic acid bacteria, 10 parts of saccharomycete, photosynthetic
7 parts of bacterium, 18 parts of composite bacillus, 10 parts of actinomyces, 25 parts of cellulase, 18 parts of protease, 20 parts of lactobacillus plantarum；Institute
Compound microorganism bacterium powder agent B is stated to be made of the component of following parts by weight：75 parts of B microbial inoculum, 15 parts of diatomite, is done 6 parts of microelement
8 parts of drying prescription, wherein B microbial inoculum is mixed by the component of following parts by weight：12 parts of denitrification desulfurization bacterium, 15 parts of nitrifier, circle is brown
22 parts of nitrogen-fixing bacteria, 25 parts of zeolite powder, 20 parts of zytase.
The preparation method is the same as that of Example 1 by compound microorganism bacterium powder agent A and compound microorganism bacterium powder agent B.
Test effect
In the fish jar of two similarity conditions, water is 350L in fish jar, and raising has the peacock of 15 equal conditions respectively
Fish is added microorganism live bacteria agent of the invention in the fish jar of test example, any microbial inoculum is not added in the fish jar of comparative example, is used as blank
Comparative test.
The following are the comparative experimental datas of test example and comparative example fish jar
11 middle of the month during test, every 3 months replacement a quarter water, and according to quantity timing launch fish
Feed, during test, the concentration of the COD and ammonia nitrogen that do not add the fish jar of microorganism live bacteria agent have increased separately 26.3% He
894%, transparency is gradually reduced, and water quality becomes cloudy, and Poecilia is gradually dead, survival rate 66.7%；And it is added to microorganism
The COD and ammonia nitrogen value of the fish jar of live bacteria agent are kept in the normal range, and water quality transparency is stablized, substantially unchanged, and pH is suitable for hole
The survival rate of Lethotremus awae is 100%, and therefore, originally experiments have shown that, which can be effectively reduced ammonia nitrogen, hydrogen sulfide, Asia
Nitrate and COD value stablize pH value, improve photosynthetic capacity, improve water body color, increase water transparency, improve fish
Survival rate, improve the exterior quality of fish, therefore indoor fish jar feeding fish field can be widely used in.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those skilled in the art,
Without depart from that overall concept of the invention, several changes and improvements can also be made, these also should be considered as of the invention
Protection scope.
Claims (6)
1. a kind of microorganism live bacteria agent for fish raising, it is characterised in that：Including compound microorganism bacterium powder agent A and composite microbial
Object bacteria powder B, the compound microorganism bacterium powder agent A are made of the component of following parts by weight：65-75 parts of A microbial inoculum, sodium alginate 8-
12 parts, 8-12 parts of polyvinyl alcohol, 5-10 parts of desiccant, wherein A microbial inoculum is mixed by the component of following parts by weight：Lactic acid bacteria
20-30 parts, 6-12 parts of saccharomycete, 4-8 parts of photosynthetic bacteria, 10-18 parts of composite bacillus, 7-13 parts of actinomyces, cellulase
20-30 parts, 15-28 parts of protease, 18-25 parts of lactobacillus plantarum；The compound microorganism bacterium powder agent B by following parts by weight group
Divide and is made：65-75 parts of B microbial inoculum, 2-10 parts of microelement, 10-20 parts of diatomite, 5-10 parts of desiccant, wherein B microbial inoculum is by following
The component of parts by weight is mixed：8-15 parts of denitrification desulfurization bacterium, 8-15 parts of nitrifier, 15-25 parts of azotobacter chroococcum, zeolite powder
25-40 parts, 15-20 parts of zytase.
2. the microorganism live bacteria agent for fish raising as described in claim 1, it is characterised in that：The compound microorganism bacterium powder
Agent A is prepared by following methods：
(1) culture of microbial inoculum：It is 28-32 DEG C that temperature, which is arranged, by all components strain of A microbial inoculum in activation medium
Inoculation, activation culture 24-48h, the activation medium is by soy peptone 8g, glucose 20g, yeast powder 20g, water 1000ml
It is configured to culture solution, pH is adjusted to neutrality, and cultured strain ferments in the fermentation medium then and expands numerous 24-72h, described
Fermentation medium is peptone 5kg, yeast powder 0.8kg, glucose 15kg, magnesium sulfate 1kg, potassium dihydrogen phosphate 0.3kg, phosphoric acid hydrogen
Dipotassium 0.3kg, ammonium sulfate 1kg, sodium citrate 0.05kg, iron chloride 0.05kg, cobalt chloride 0.02kg, copper sulphate 0.03kg, chlorine
Change zinc 0.05kg, calcium chloride 0.05kg, vitaminB10 .05kg, vitamin B2 0.05kg, calcium pantothenate 0.03kg, folic acid
0.03kg, niacin 0.03kg are formulated, and are adjusted pH7.5, are settled to 1t with water.During activation culture and fermentation expand numerous
It constantly shakes or stirs, after obtaining bacterium solution, be washed with distilled water thallus under centrifugation, concentration, room temperature, obtain A microbial inoculum；
(2) the resulting A microbial inoculum of step (1) is dried in the preparation of complex micro organism fungicide, and drying temperature is 40-50 DEG C, crushed
Then the A bacterium powder is uniformly mixed by 120 meshes with the sodium alginate, polyvinyl alcohol, desiccant crushed, air drying obtains
Compound microorganism bacterium powder agent A.
3. the microorganism live bacteria agent for fish raising as described in claim 1, it is characterised in that：The compound microorganism bacterium powder
Agent B is prepared by following methods：
(1) culture of microbial inoculum：It is 30-35 DEG C that temperature, which is arranged, by all components strain of B microbial inoculum in activation medium
Inoculation, activation culture 24-48h, the activation medium is by soy peptone 8g, glucose 20g, yeast powder 20g, water 1000ml
It is configured to culture solution, pH is adjusted to neutrality, and cultured strain ferments in the fermentation medium then and expands numerous 24-72h, described
Fermentation medium is peptone 5kg, yeast powder 0.8kg, glucose 15kg, magnesium sulfate 1kg, potassium dihydrogen phosphate 0.3kg, phosphoric acid hydrogen
Dipotassium 0.3kg, ammonium sulfate 1kg, sodium citrate 0.05kg, iron chloride 0.05kg, cobalt chloride 0.02kg, copper sulphate 0.03kg, chlorine
Change zinc 0.05kg, calcium chloride 0.05kg, vitaminB10 .05kg, vitamin B2 0.05kg, calcium pantothenate 0.03kg, folic acid
0.03kg, niacin 0.03kg are formulated, and are adjusted pH7.5, are settled to 1t with water.During activation culture and fermentation expand numerous
It constantly shakes or stirs, after obtaining bacterium solution, be washed with distilled water thallus under centrifugation, concentration, room temperature, obtain B microbial inoculum；
(2) the resulting B microbial inoculum of step (1) is dried in the preparation of complex micro organism fungicide, and drying temperature is 40-50 DEG C, crushed
Then obtained B bacterium powder is uniformly mixed, room temperature by 120 meshes with calcium carbonate, microelement, diatomite, the desiccant crushed
It is dry, obtain compound microorganism bacterium powder agent B.
4. the microorganism live bacteria agent for fish raising as described in claim 1, it is characterised in that：The microelement is by sulfuric acid
Zinc, magnesium sulfate and potassium dihydrogen phosphate=1：1：1 composition；The desiccant is in bentonite, silica gel, white carbon black or attapulgite
It is a kind of.
5. the microorganism live bacteria agent for fish raising as claimed in claim 2 or claim 3, it is characterised in that：First the activation is trained
It supports base and produces fermentation medium respectively after 115-125 DEG C of high-temperature sterilization 30-50min, cultivated strain is added.
6. the application method for the microorganism live bacteria agent of fish raising as described in claim 1, it is characterised in that：Every liter of water is applied
Dosage is 0.1g, first applies compound microorganism bacterium powder agent A, applies compound microorganism bacterium powder agent B after 3-5 days again.
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| CN110002609A (en) * | 2019-04-18 | 2019-07-12 | 山东省食品药品检验研究院 | A kind of microorganism improver of water quality and preparation method thereof |
| CN110482712A (en) * | 2019-07-18 | 2019-11-22 | 国家地质实验测试中心 | A kind of Water environment restoration material and preparation method |
| CN110980942A (en) * | 2019-12-10 | 2020-04-10 | 浙江永续环境工程有限公司 | Anaerobic biological agent and anaerobic treatment method using same |
| CN112456653A (en) * | 2020-10-27 | 2021-03-09 | 广西诺亚生物科技发展有限公司 | Compound for adjusting aquaculture water quality and preparation process thereof |
| CN114731980A (en) * | 2022-04-27 | 2022-07-12 | 珠海麦得发生物科技股份有限公司 | Bacterial liquid compound for assisting in improving bacterial infection in vitro of fish and application thereof |
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| CN110002609A (en) * | 2019-04-18 | 2019-07-12 | 山东省食品药品检验研究院 | A kind of microorganism improver of water quality and preparation method thereof |
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