CN108991281A - A method of koi feed is produced using Pig farm wastewater cultivating microalgae - Google Patents

A method of koi feed is produced using Pig farm wastewater cultivating microalgae Download PDF

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CN108991281A
CN108991281A CN201810743029.7A CN201810743029A CN108991281A CN 108991281 A CN108991281 A CN 108991281A CN 201810743029 A CN201810743029 A CN 201810743029A CN 108991281 A CN108991281 A CN 108991281A
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microalgae
pig farm
feed
farm wastewater
koi
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江继永
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江继永
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Abstract

The present invention provides a kind of method using Pig farm wastewater cultivating microalgae production koi feed, it is related to cultural technique field, comprising the following steps: (1) cultivate what is cultivated in advance in logarithmic growth phase concentration to be expanded after the microalgae liquid alkaline acclimation of 1-1.5g/L with sewage;(2) dilution, sterilizing after Pig farm wastewater precipitating, microalgae is seeded in sewage and is cultivated;(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtained concentration algae solution, after fermentation, obtain Microalgae fermentation liquid;Make koi feed, the microalgae carotenoid content height cultivated in the present invention using Pig farm wastewater, for making koi feed, with lower feed coefficient and the death rate, fancy carp growth speed is improved, fancy carp posture can also be improved, make its more grossness, fancy carp color is more gorgeous, microalgae can purify sewage, reduce the environmental protection pressure on pig farm, achieve many things at one stroke, it is environmentally protective, meet the requirement of sustainable development.

Description

A method of koi feed is produced using Pig farm wastewater cultivating microalgae
Technical field
The present invention relates to cultural technique fields, and in particular to a kind of to produce koi feed using Pig farm wastewater cultivating microalgae Method.
Background technique
In recent years, with new countryside construction, scale animal and poultry cultivation industry is rapidly developed, farm animal excrement yield with Quickly increase.Breeding wastewater mainly includes animals urine, excrement and cultivation column flushing water, is contained in breeding wastewater a large amount of Nitrogen, phosphorus, amine can cause water pollution, cause water eutrophication, lead to blue algae bloom if direct emission, cause ecological danger Machine.
Only the whole nation of the first half of the year in 2017 just as environment protection treating and close 21.3 ten thousand pig farms, wherein there are about 80% to be Because breeding wastewater disorderly discharges, China pig farm has and is greatly farmer development sideline production and self-built, the pass of unilateral single solution for diverse problems formula Peasant's foundation enthusiasm will be hit by closing, and also powerlessly purchase runs sewage disposal device on middle-size and small-size pig farm.
Fancy carp (Cyprinus carpio L.) athletic looking, beautiful in colour, decorative pattern is various, with high ornamental and feeding Support value.Selection cross of the fancy carp after many years, has formed some very characteristic types at present, and ornamental value depends on mostly In the color of body surface, research shows that the nutritional ingredient in feed can have an impact fancy carp body surface color.
Microalgae is that have the protophyte of photosynthetic ability, and micro-algae propagation survival ability is extremely strong, can be from waste water Absorb various inorganic elements and organic matter, can be used for a large amount of nitrogen phosphorus in absorption cleaning aquaculture wastewater, and microalgae contain it is more Kind vitamin and protein and polysaccharide material, carotenoid, can be used for manufacturing koi feed, can both reduce fancy carp and support Cost is grown, the color of body surface is improved, and the ingredient of nutriment in feed can be improved, is to achieve many things at one stroke.
Application publication number is that the Chinese patent of 107629961 A discloses a kind of side using breeding wastewater culture microalgae Method comprising following steps: (1) microalgae is tamed: microalgae being inoculated into culture medium and is cultivated, is added during the cultivation process not The breeding wastewater of sterilized processing, and step up according to the increased number of microalgae the adding proportion of breeding wastewater;(2) microalgae is living Change: the breeding wastewater of non-sterilized processing is added in the medium, then by the microalgae after domestication be inoculated in the culture medium into Row expands culture;(3) microdisk electrode: the microalgae after activation is inoculated into the breeding wastewater solution of non-sterilized processing, at family It is cultivated under the conditions of outer, realizes cultivation wastewater purification and microalgae grease production.Also, it is useless using the cultivation of non-sterilized processing Water outdoors under the conditions of cultivate microalgae, can be achieved at the same time cultivation wastewater purification and microalgae grease production, have low in cost, energy The advantages of being enough mass produced, but urine, excrement in Pig farm wastewater containing pig, wherein contain a large amount of amine substance, PH higher directly raises and train microalgae with the breeding wastewater of non-sterilized processing in the above method, do not obtain in advance it is high resistance to In the case where the microalgae of alkali, it is easy to cause the large quantities of death of microalgae, be unfavorable for the expansion culture and feed manufacture in later period.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, koi feed is produced using Pig farm wastewater cultivating microalgae the present invention provides a kind of Method.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs:
A method of koi feed is produced using Pig farm wastewater cultivating microalgae, comprising the following steps:
(1) it is inoculated into culture solution, trains in the microalgae liquid that logarithmic growth phase concentration is 1-1.5g/L by what is cultivated in advance Nutrient solution PH is 6.5-7.5, and lye adjusting culture solution PH to 8.5 is added after cultivating 4h, is hereafter adjusted and is cultivated with lye again every 4h Liquid PH is primary, and culture solution PH is respectively 9.5,10.5, and the microalgae after finally obtaining alkali acclimation will be micro- after alkali acclimation Algae expands culture to 5 × 10 with 50-100 times of diluted Pig farm wastewater6cells/mL;
(2) clear liquid is pumped into conditioning tank after Pig farm wastewater precipitating, is diluted with water to ammonia-nitrogen content≤500mg/L, total phosphorus contains Bleaching powder is added into sewage and carries out sterilizing, microalgae is seeded in sewage after 4-6d for amount≤120mg/L, PH≤10.5, Both culturing microalgae is carried out, control sewage temperature is 20-30 DEG C, intensity of illumination 4000-6000lux, Light To Dark Ratio 1:1 when cultivation;
(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtain concentration algae solution, added into concentration algae solution Enter fermenting microbe to ferment, 30-35 DEG C of fermentation temperature, speed of agitator 300-350r/min, filtrated air intake is 0.45-0.6vvm, ferment 4-10d, obtains Microalgae fermentation liquid;
Filtrate carry out composition detection, meet GB 18596-2001 country's livestock and poultry breeding industry discharge new standard, can directly into Row discharge, if do not met, may be used as dilution of sewage in step (2);
(4) by Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, microalgae Fermentation liquid is added in mixing machine after crushing respectively and is uniformly mixed, and water is added to adjust mixture moisture content to 20-25%, by mixture It is added in double screw extruder, the pressure in extruder sleeve is 2.5-3.5MPa, and temperature is 130-150 DEG C, and extruded stock is cut The koi feed can be obtained after grain, surface spraying phagostimulant, drying and sterilizing.
The microalgae can be any one in scenedesmus, purple ball algae, spirulina and chlorella, preferably spirulina.
Preferably, the culture solution is by following weight percent at being grouped as: sodium nitrate 10-12%, potassium hydrogen phosphate 0.5-0.8%, magnesium sulfate 0.25-0.4%, citric acid 0.54-0.66%, ferric citrate 0.01-0.05%, ethylenediamine tetrem Acid disodium 0.01-0.015%, calcium chloride 0.25-0.36%, sodium carbonate 0.1-0.2%, boric acid 0.01-0.025%, manganese chloride 0.01-0.016%, zinc sulfate 0.01-0.02%, copper sulphate 0.01-0.05%, sodium molybdate 0.01-0.035%, remaining is water.
Preferably, the lye is sodium carbonate liquor or sodium hydroxide solution.
Preferably, the mass concentration 15-22% of the sodium carbonate liquor, the mass concentration of the sodium hydroxide solution are 0.15-0.3%.
Preferably, the additional amount of bleaching powder is 35-50mg/L.
Preferably, fermenting microbe is activated in advance, method particularly includes: fermenting microbe is put into equipped with culture In the conical flask of base, then conical flask is placed on shaking table, 26 DEG C, 150r/min, activates 20-25h.
Preferably, the culture medium is by following weight percent at being grouped as: glucose 40-60%, natural sea salt 10- 20%, yeast extract 3-5%, peptone 4-8%, agar powder 15-30%.
Preferably, Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, micro- The weight ratio of algae fermentation liquid is 20-40:1-5:5-10:5-10:1-5:1-5:1-5:1.5-2.5:10-15.
Preferably, the Multi-vitamin and multi-mineral premix is that dimension promise crowd supports, and is purchased from Zhuhai City Wei Nuo breeding Co., Ltd or favorite With, be purchased from the favorite Bioisystech Co., Ltd of Wuxi City.
Preferably, the phagostimulant is food calling raw meat peptide and glycine betaine, and the two weight ratio is 1:1, is pre-added to before spraying In fish oil, then spray to feed surface.
Preferably, the temperature of drying is 70-75 DEG C, the water content 8-12% of feed after drying.
(3) beneficial effect
The present invention provides a kind of methods using Pig farm wastewater cultivating microalgae production koi feed, have beneficial below Effect:
Microalgae protein content in the present invention using Pig farm wastewater cultivation is high, and rich in nutrition content, carotenoid contains Amount is high, for making koi feed, has lower feed coefficient and the death rate, improves fancy carp growth speed, can also improve Fancy carp posture makes its more grossness, feeds fancy carp fish-skin after a period of time, the content in dorsal fin for the carotenoid of coloring Also it increased, fancy carp color is more gorgeous, in addition, microalgae is in the breeding process, can purify sewage, reduce ammonia nitrogen in sewage Content, total phosphorus content, COD, purified sewage can be reduced the environmental protection pressure on pig farm, be achieved many things at one stroke with direct emission, green Colour circle is protected, and the requirement of sustainable development is met.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention, Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention one Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of creative work.
Embodiment 1:
A method of koi feed is produced using Pig farm wastewater cultivating microalgae, comprising the following steps:
(1) it is inoculated into culture solution, trains in the spirulina liquid that logarithmic growth phase concentration is 1.2g/L by what is cultivated in advance Nutrient solution PH is 6.5-7.5, cultivates the sodium carbonate liquor adjusting culture solution PH to 8.5 of addition mass concentration 15% after 4h, hereafter often Primary with sodium carbonate liquor adjusting culture solution PH again every 4h, culture solution PH is respectively 9.5,10.5, finally obtains alkali acclimation 80 times of diluted Pig farm wastewaters of microalgae after alkali acclimation are expanded culture to 5 × 10 by microalgae afterwards6cells/mL;
Culture solution is by following weight percent at being grouped as: sodium nitrate 10.2%, potassium hydrogen phosphate 0.56%, magnesium sulfate 0.28%, citric acid 0.58%, ferric citrate 0.023%, disodium ethylene diamine tetraacetate 0.013%, calcium chloride 0.26%, carbon Sour sodium 0.13%, boric acid 0.022%, manganese chloride 0.012%, zinc sulfate 0.014%, copper sulphate 0.037%, sodium molybdate 0.026%, remaining is water;
(2) clear liquid is pumped into conditioning tank after Pig farm wastewater precipitating, is diluted with water to ammonia-nitrogen content 382mg/L, total phosphorus content 40mg/L bleaching powder is added into sewage and carries out sterilizing, microalgae is seeded in sewage after 5d by 113mg/L, PH 9.7, into Row both culturing microalgae, control sewage temperature is 20-30 DEG C, intensity of illumination 5000lux, Light To Dark Ratio 1:1 when cultivation;
(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtain concentration algae solution, added into concentration algae solution Enter Angel flavor type koji HY12-11 to ferment, 32 DEG C of fermentation temperature, speed of agitator 330r/min, filtrated air is logical Entering amount is 0.48vvm, and ferment 8d, obtains Microalgae fermentation liquid;
Filtrate carries out composition detection (GB 18596-2001 country's livestock and poultry breeding industry discharges new standard), ammonia-nitrogen content 63mg/ L, total phosphorus content 7.8mg/L, COD 365mg/L can be discharged directly;
Fermenting microbe is activated in advance, method particularly includes: fermenting microbe is put into the cone equipped with culture medium In shape bottle, then conical flask is placed on shaking table, 26 DEG C, 150r/min, activates 22h;Culture medium is by following weight percent At being grouped as: glucose 50%, natural sea salt 15%, yeast extract 4%, peptone 8%, agar powder 23%;
(4) by Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, microalgae Fermentation liquid is uniformly mixed by weight being added in mixing machine for 30:2:8:8:1:2:2:2:12 after crushing respectively, and water is added to adjust Mixture moisture content adds mixture into double screw extruder, the pressure in extruder sleeve is 3MPa, temperature to 22% It is 140 DEG C, food calling raw meat peptide and glycine betaine weight ratio are that 1:1 is added in fish oil, then sprays to feed table by extruded stock pelletizing Face, 72 DEG C of drying, can be obtained the koi feed after the water content 7.3% of feed, sterilization after drying.
Embodiment 2:
A method of koi feed is produced using Pig farm wastewater cultivating microalgae, comprising the following steps:
(1) it is inoculated into culture solution, cultivates in the spirulina liquid that logarithmic growth phase concentration is 1g/L by what is cultivated in advance Liquid PH be 6.5-7.5, cultivate 4h after be added mass concentration 22% sodium carbonate liquor adjust culture solution PH to 8.5, hereafter every 4h is primary with sodium carbonate liquor adjusting culture solution PH again, and culture solution PH is respectively 9.5,10.5, after finally obtaining alkali acclimation Microalgae, 50 times of diluted Pig farm wastewaters of the microalgae after alkali acclimation are expanded into culture to 5 × 106cells/mL;
Culture solution is by following weight percent at being grouped as: sodium nitrate 10%, potassium hydrogen phosphate 0.5%, magnesium sulfate 0.25%, citric acid 0.54%, ferric citrate 0.01%, disodium ethylene diamine tetraacetate 0.01%, calcium chloride 0.25%, carbonic acid Sodium 0.1%, boric acid 0.01%, manganese chloride 0.01%, zinc sulfate 0.01%, copper sulphate 0.01%, sodium molybdate 0.01%, remaining is Water;
(2) clear liquid is pumped into conditioning tank after Pig farm wastewater precipitating, is diluted with water to ammonia-nitrogen content 420, total phosphorus content 45mg/L bleaching powder is added into sewage and carries out sterilizing, microalgae is seeded in sewage after 4d by 103mg/L, PH 9.5, into Row both culturing microalgae, control sewage temperature is 20-30 DEG C, intensity of illumination 4000lux, Light To Dark Ratio 1:1 when cultivation;
(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtain concentration algae solution, added into concentration algae solution Entering fermenting microbe is that Angel flavor type koji HY12-11 ferments, 30 DEG C of fermentation temperature, speed of agitator 300r/min, Filtrated air intake is 0.45vvm, and ferment 4d, obtains Microalgae fermentation liquid;
Filtrate carries out composition detection (GB 18596-2001 country's livestock and poultry breeding industry discharges new standard), ammonia-nitrogen content 82mg/ L, total phosphorus content 7.5mg/L, COD382mg/L cannot direct emission, storage is used as next aquaculture wastewater dilution and uses;
Fermenting microbe is activated in advance, method particularly includes: fermenting microbe is put into the cone equipped with culture medium In shape bottle, then conical flask is placed on shaking table, 26 DEG C, 150r/min, activates 25h;Culture medium is by following weight percent At being grouped as: glucose 60%, natural sea salt 10%, yeast extract 3%, peptone 4%, agar powder 23%;
(4) by Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, microalgae Fermentation liquid is uniformly mixed by weight being added in mixing machine for 25:1:5:10:1:3:1:2:12 after crushing respectively, and water is added to adjust Mixture moisture content adds mixture into double screw extruder, the pressure in extruder sleeve is 3.2MPa, temperature to 20% Degree is 135 DEG C, and food calling raw meat peptide and glycine betaine weight ratio are that 1:1 is added in fish oil, then sprays to feed table by extruded stock pelletizing Face, 75 DEG C of drying, can be obtained the koi feed after the water content 7.8% of feed, sterilization after drying.
Embodiment 3:
A method of koi feed is produced using Pig farm wastewater cultivating microalgae, comprising the following steps:
(1) it is inoculated into culture solution, trains in the spirulina liquid that logarithmic growth phase concentration is 1.5g/L by what is cultivated in advance Nutrient solution PH is 6.5-7.5, and the sodium hydroxide solution adjusting culture solution PH to 8.5 that mass concentration is 0.15% is added after cultivating 4h, Hereafter primary with sodium hydroxide solution adjusting culture solution PH again every 4h, culture solution PH is respectively 9.5,10.5, finally obtains alkali Microalgae after acclimation, by 100 times of diluted Pig farm wastewaters of the microalgae after alkali acclimation expand culture to 5 × 106cells/mL;
Culture solution is by following weight percent at being grouped as: sodium nitrate 12%, potassium hydrogen phosphate 0.8%, magnesium sulfate 0.4%, citric acid 0.66%, ferric citrate 0.05%, disodium ethylene diamine tetraacetate 0.015%, calcium chloride 0.36%, carbonic acid Sodium 0.2%, boric acid 0.025%, manganese chloride 0.016%, zinc sulfate 0.02%, copper sulphate 0.05%, sodium molybdate 0.035%, Yu Weishui;
(2) clear liquid is pumped into conditioning tank after Pig farm wastewater precipitating, is diluted with water to ammonia-nitrogen content 500mg/L, total phosphorus content 50mg/L bleaching powder is added into sewage and carries out sterilizing, microalgae is seeded in sewage after 6d by 120mg/L, PH 10.5, Both culturing microalgae is carried out, control sewage temperature is 20-30 DEG C, intensity of illumination 6000lux, Light To Dark Ratio 1:1 when cultivation;
(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtain concentration algae solution, added into concentration algae solution Entering fermenting microbe is that Angel flavor type koji HY12-11 ferments, 35 DEG C of fermentation temperature, speed of agitator 350r/min, Filtrated air intake is 0.6vvm, and ferment 10d, obtains Microalgae fermentation liquid;
Filtrate carries out composition detection (GB 18596-2001 country's livestock and poultry breeding industry discharges new standard), ammonia-nitrogen content 85mg/ L, total phosphorus content 8.1mg/L, COD346mg/L cannot direct emission, storage is used as next aquaculture wastewater dilution and uses;
Fermenting microbe is activated in advance, method particularly includes: fermenting microbe is put into the cone equipped with culture medium In shape bottle, then conical flask is placed on shaking table, 26 DEG C, 150r/min, activates 20h;Culture medium is by following weight percent At being grouped as: glucose 40%, natural sea salt 20%, yeast extract 3%, peptone 7%, agar powder 30%;
(4) by Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, microalgae Fermentation liquid is uniformly mixed by weight being added in mixing machine for 20:1:5:5:1:1:1:1.5:10 after crushing respectively, adds water tune Mixture moisture content is saved to 20%, is added mixture into double screw extruder, the pressure in extruder sleeve is 2.5MPa, Temperature is 130 DEG C, and food calling raw meat peptide and glycine betaine weight ratio are that 1:1 is added in fish oil, then sprays to feed by extruded stock pelletizing Surface, 70 DEG C of drying, can be obtained the koi feed after the water content 6.5% of feed, sterilization after drying.
Embodiment 4:
A method of koi feed is produced using Pig farm wastewater cultivating microalgae, comprising the following steps:
(1) it is inoculated into culture solution, cultivates in the spirulina liquid that logarithmic growth phase concentration is 1g/L by what is cultivated in advance Liquid PH is 6.5-7.5, the sodium hydroxide solution adjusting culture solution PH to 8.5 that mass concentration is 0.3% is added after cultivating 4h, hereafter Primary with sodium hydroxide solution adjusting culture solution PH again every 4h, culture solution PH is respectively 9.5,10.5, finally obtains alkali domestication Microalgae that treated, by 100 times of diluted Pig farm wastewaters of the microalgae after alkali acclimation expand culture to 5 × 106cells/mL;
Culture solution is by following weight percent at being grouped as: sodium nitrate 10%, potassium hydrogen phosphate 0.5%, magnesium sulfate 0.3%, citric acid 0.6%, ferric citrate 0.01%, disodium ethylene diamine tetraacetate 0.015%, calcium chloride 0.35%, carbonic acid Sodium 0.1%, boric acid 0.01%, manganese chloride 0.016%, zinc sulfate 0.02%, copper sulphate 0.05%, sodium molybdate 0.01%, remaining For water;
(2) clear liquid is pumped into conditioning tank after Pig farm wastewater precipitating, is diluted with water to ammonia-nitrogen content 460mg/L, total phosphorus content 35mg/L bleaching powder is added into sewage and carries out sterilizing, microalgae is seeded in sewage after 6d by 109mg/L, PH10.3, into Row both culturing microalgae, control sewage temperature is 20-30 DEG C, intensity of illumination 6000lux, Light To Dark Ratio 1:1 when cultivation;
(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtain concentration algae solution, added into concentration algae solution Entering fermenting microbe is that Angel flavor type koji HY12-11 ferments, 30 DEG C of fermentation temperature, speed of agitator 300r/min, Filtrated air intake is 0.55vvm, and ferment 5d, obtains Microalgae fermentation liquid;
Filtrate carries out composition detection (GB 18596-2001 country's livestock and poultry breeding industry discharges new standard), ammonia-nitrogen content 80mg/ L, total phosphorus content 8.5mg/L, COD 403mg/L cannot direct emission, storage is used as next aquaculture wastewater dilution and uses;
Fermenting microbe is activated in advance, method particularly includes: fermenting microbe is put into the cone equipped with culture medium In shape bottle, then conical flask is placed on shaking table, 26 DEG C, 150r/min, activates 23h;Culture medium is by following weight percent At being grouped as: glucose 50%, natural sea salt 20%, yeast extract 5%, peptone 5%, agar powder 20%;
(4) by Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, microalgae Fermentation liquid is uniformly mixed by weight being added in mixing machine for 40:5:10:10:5:5:5:2.5:13 after crushing respectively, adds water Mixture moisture content is adjusted to 25%, is added mixture into double screw extruder, the pressure in extruder sleeve is 3.5MPa, temperature are 150 DEG C, and food calling raw meat peptide and glycine betaine weight ratio are that 1:1 is added in fish oil, then is sprayed by extruded stock pelletizing It is coated onto feed surface, 75 DEG C of drying, the koi feed can be obtained after the water content 6.6% of feed, sterilization after drying.
Embodiment 5:
A method of koi feed is produced using Pig farm wastewater cultivating microalgae, comprising the following steps:
(1) it is inoculated into culture solution, trains in the spirulina liquid that logarithmic growth phase concentration is 1.5g/L by what is cultivated in advance Nutrient solution PH is 6.5-7.5, and the sodium hydroxide solution adjusting culture solution PH to 8.5 that mass concentration is 0.3% is added after cultivating 4h, this Primary with sodium hydroxide solution adjusting culture solution PH again every 4h afterwards, culture solution PH is respectively 9.5,10.5, finally obtains alkali and tames and dociles Change treated microalgae, 70 times of diluted Pig farm wastewaters of the microalgae after alkali acclimation are expanded cultivate to 5 × 106cells/mL;
Culture solution is by following weight percent at being grouped as: sodium nitrate 11.2%, potassium hydrogen phosphate 0.67%, magnesium sulfate 0.33%, citric acid 0.58%, ferric citrate 0.04%, disodium ethylene diamine tetraacetate 0.013%, calcium chloride 0.27%, carbon Sour sodium 0.11%, boric acid 0.021%, manganese chloride 0.013%, zinc sulfate 0.012%, copper sulphate 0.03%, sodium molybdate 0.02%, Remaining is water;
(2) clear liquid is pumped into conditioning tank after Pig farm wastewater precipitating, is diluted with water to ammonia-nitrogen content 450mg/L, total phosphorus content 42mg/L bleaching powder is added into sewage and carries out sterilizing, microalgae is seeded in sewage after 5d by 115mg/L, PH 9.8, into Row both culturing microalgae, control sewage temperature is 20-30 DEG C, intensity of illumination 5000lux, Light To Dark Ratio 1:1 when cultivation;
(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtain concentration algae solution, added into concentration algae solution Entering fermenting microbe is that Angel flavor type koji HY12-11 ferments, 30 DEG C of fermentation temperature, speed of agitator 350r/min, Filtrated air intake is 0.55vvm, and ferment 6d, obtains Microalgae fermentation liquid;
Filtrate carries out composition detection (GB 18596-2001 country's livestock and poultry breeding industry discharges new standard), ammonia-nitrogen content 80mg/ L, total phosphorus content 7.5mg/L, COD 397mg/L can be with direct emissions;
Fermenting microbe is activated in advance, method particularly includes: fermenting microbe is put into the cone equipped with culture medium In shape bottle, then conical flask is placed on shaking table, 26 DEG C, 150r/min, activates 20h;Culture medium is by following weight percent At being grouped as: glucose 50%, natural sea salt 12%, yeast extract 3%, peptone 5%, agar powder 30%;
(4) by Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, microalgae Fermentation liquid is uniformly mixed by weight being added in mixing machine for 40:1:5:5:1:1:1:1.5:15 after crushing respectively, adds water tune Mixture moisture content is saved to 20%, is added mixture into double screw extruder, the pressure in extruder sleeve is 3.5MPa, Temperature is 140 DEG C, and food calling raw meat peptide and glycine betaine weight ratio are that 1:1 is added in fish oil, then sprays to feed by extruded stock pelletizing Surface, 75 DEG C of drying, can be obtained the koi feed after the water content 7.4% of feed, sterilization after drying.
Protein and carotenoid content detection in microalgae:
Protein and carotenoid content testing result pair in the microalgae that the following table 1 is cultivated by 1-5 of the embodiment of the present invention Than:
Table 1:
Nutrient composition content detection comparison:
The following table 2 is koi feed prepared by 1-3 of the embodiment of the present invention and commercially available koi feed (purchased from Weifang meaning product pet Articles Co., Ltd, brand yee, article No.: A002) nutrient composition content comparing result:
Table 2:
Koi feed prepared by 1-3 of the embodiment of the present invention (is doted on the commercially available koi feed of commercial diet purchased from Weifang meaning product Object articles Co., Ltd, brand yee, article No.: A002) known to nutritional ingredient comparison: crude protein content is close, grease, crude fibre, Crude ash content is higher than commercial diet, and lysine content is lower than commercial diet.
Feed experiment:
Two 4m × 2m × 4m cement pits are built, experiment is fed and is carried out in cement pit, each cement pit respectively puts brocade in a suitable place to breed 80 tail of carp, respectively first group and second group, wherein first group is fed with koi feed prepared by the embodiment of the present invention 1, and second group is with commercially available Feedstuff feeding is fed 2 times daily, and feeding volume is about the 3% of weight, and Feeding time is respectively 10:00 and 18:00.Test is used Water is the aeration tap water above for 24 hours, and water temperature is 25 (± 2.0) DEG C, test period 60d.
It is as shown in table 3 below to feed experimental result:
Table 3:
Analysis: first group and second group compare, the experimental results showed that, koi feed prepared by the embodiment of the present invention 1 has lower Feed coefficient and the death rate, the higher speed of growth, and fancy carp posture can be improved, make its more grossness, increase fancy carp Content in fish-skin, dorsal fin for the carotenoid of coloring.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that There is also other identical elements in process, method, article or equipment including the element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although with reference to the foregoing embodiments Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation Technical solution documented by example is modified or equivalent replacement of some of the technical features;And these modification or Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.

Claims (10)

1. a kind of method using Pig farm wastewater cultivating microalgae production koi feed, which comprises the following steps:
(1) it is inoculated into what is cultivated in advance in culture solution in the microalgae liquid that logarithmic growth phase concentration is 1-1.5g/L, culture solution PH is 6.5-7.5, and lye adjusting culture solution PH to 8.5 is added after cultivating 4h, hereafter adjusts culture solution PH with lye again every 4h Once, culture solution PH is respectively 9.5,10.5, the microalgae after finally obtaining alkali acclimation, and the microalgae after alkali acclimation is used 50-100 times of diluted Pig farm wastewater expands culture to 5 × 106cells/mL;
(2) clear liquid is pumped into conditioning tank after Pig farm wastewater precipitating, is diluted with water to ammonia-nitrogen content≤500mg/L, and total phosphorus content≤ Bleaching powder is added into sewage and carries out sterilizing, microalgae is seeded in sewage after 4-6d, is carried out for 120mg/L, PH≤10.5 Both culturing microalgae, control sewage temperature is 20-30 DEG C, intensity of illumination 4000-6000lux, Light To Dark Ratio 1:1 when cultivation;
(3) sewage containing microalgae is pumped into algae-recovering bag with water pump and is filtered, obtain concentration algae solution, hair is added into concentration algae solution Yeast-like fungi kind is fermented, 30-35 DEG C of fermentation temperature, speed of agitator 300-350r/min, and filtrated air intake is 0.45- 0.6vvm, ferment 4-10d, obtains Microalgae fermentation liquid;
(4) by Strong flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, Microalgae fermentation Liquid is added in mixing machine after crushing respectively and is uniformly mixed, and adds water to adjust mixture moisture content to 20-25%, adds mixture into Into double screw extruder, the pressure in extruder sleeve is 2.5-3.5MPa, and temperature is 130-150 DEG C, extruded stock pelletizing, table The koi feed can be obtained after face spraying phagostimulant, drying and sterilizing.
2. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as described in claim 1, which is characterized in that institute Culture solution is stated by following weight percent at being grouped as: sodium nitrate 10-12%, potassium hydrogen phosphate 0.5-0.8%, magnesium sulfate 0.25-0.4%, citric acid 0.54-0.66%, ferric citrate 0.01-0.05%, disodium ethylene diamine tetraacetate 0.01- 0.015%, calcium chloride 0.25-0.36%, sodium carbonate 0.1-0.2%, boric acid 0.01-0.025%, manganese chloride 0.01- 0.016%, zinc sulfate 0.01-0.02%, copper sulphate 0.01-0.05%, sodium molybdate 0.01-0.035%, remaining is water.
3. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as described in claim 1, which is characterized in that institute Stating lye is sodium carbonate liquor or sodium hydroxide solution.
4. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as claimed in claim 3, which is characterized in that institute The mass concentration 15-22% of sodium carbonate liquor is stated, the mass concentration of the sodium hydroxide solution is 0.15-0.3%.
5. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as described in claim 1, which is characterized in that drift The additional amount of white powder is 35-50mg/L.
6. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as described in claim 1, which is characterized in that hair Yeast-like fungi kind is activated in advance, method particularly includes: fermenting microbe is put into the conical flask equipped with culture medium, then will Conical flask is placed on shaking table, 26 DEG C, 150r/min, activates 20-25h.
7. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as claimed in claim 6, which is characterized in that institute Culture medium is stated by following weight percent at being grouped as: glucose 40-60%, natural sea salt 10-20%, yeast extract 3-5%, Peptone 4-8%, agar powder 15-30%.
8. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as described in claim 1, which is characterized in that high Gluten flour, lecithin, soybean powder, soya-bean cake, shrimp med, laver powder, fish meal, Multi-vitamin and multi-mineral premix, Microalgae fermentation liquid weight ratio For 20-40:1-5:5-10:5-10:1-5:1-5:1-5:1.5-2.5:10-15.
9. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as described in claim 1, which is characterized in that institute Stating phagostimulant is food calling raw meat peptide and glycine betaine, and the two weight ratio is 1:1, is pre-added in fish oil before spraying, then spray to feeding Expect surface.
10. utilizing the method for Pig farm wastewater cultivating microalgae production koi feed as described in claim 1, which is characterized in that The temperature of drying is 70-75 DEG C, the water content 6-8% of feed after drying.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112591891A (en) * 2020-12-02 2021-04-02 中国海洋大学 Bacteria and algae cooperation system for culture tail water treatment

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101074144A (en) * 2006-05-17 2007-11-21 郭文瑞 Ecological treatment and recovering utilization for farm
CN102010830A (en) * 2010-08-26 2011-04-13 北京芳能科技有限公司 Spirulina acclimatization method and application thereof
CN102161550A (en) * 2011-01-13 2011-08-24 蔡志武 Method for producing feed additive from livestock and poultry breeding wastewater and purifying breeding wastewater to reclaimed water
CN105753245A (en) * 2014-12-19 2016-07-13 宋维成 Method for treating poultry farm wastewater by using chlorella and chitosan
CN105754860A (en) * 2016-03-31 2016-07-13 湖南大学 Method for efficiently purifying biogas slurry in pig farm through composite microalgae
CN105776745A (en) * 2016-04-05 2016-07-20 湖南大学 Biological treatment method of high-ammonia nitrogen pig raising biogas slurry
CN107467372A (en) * 2017-07-31 2017-12-15 兰溪市酉泽饲料技术服务有限公司 The preparation method of fancy carp adult fish phase feed
CN108249574A (en) * 2017-12-13 2018-07-06 华南理工大学 It is a kind of to handle pig farm breeding wastewater using microalgae and produce the device and its technique of algae powder

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101074144A (en) * 2006-05-17 2007-11-21 郭文瑞 Ecological treatment and recovering utilization for farm
CN102010830A (en) * 2010-08-26 2011-04-13 北京芳能科技有限公司 Spirulina acclimatization method and application thereof
CN102161550A (en) * 2011-01-13 2011-08-24 蔡志武 Method for producing feed additive from livestock and poultry breeding wastewater and purifying breeding wastewater to reclaimed water
CN105753245A (en) * 2014-12-19 2016-07-13 宋维成 Method for treating poultry farm wastewater by using chlorella and chitosan
CN105754860A (en) * 2016-03-31 2016-07-13 湖南大学 Method for efficiently purifying biogas slurry in pig farm through composite microalgae
CN105776745A (en) * 2016-04-05 2016-07-20 湖南大学 Biological treatment method of high-ammonia nitrogen pig raising biogas slurry
CN107467372A (en) * 2017-07-31 2017-12-15 兰溪市酉泽饲料技术服务有限公司 The preparation method of fancy carp adult fish phase feed
CN108249574A (en) * 2017-12-13 2018-07-06 华南理工大学 It is a kind of to handle pig farm breeding wastewater using microalgae and produce the device and its technique of algae powder

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112591891A (en) * 2020-12-02 2021-04-02 中国海洋大学 Bacteria and algae cooperation system for culture tail water treatment

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Application publication date: 20181214