CN1144844A - Manufacturing method of iron-rich pure spirulina - Google Patents

Manufacturing method of iron-rich pure spirulina Download PDF

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Publication number
CN1144844A
CN1144844A CN95109095A CN95109095A CN1144844A CN 1144844 A CN1144844 A CN 1144844A CN 95109095 A CN95109095 A CN 95109095A CN 95109095 A CN95109095 A CN 95109095A CN 1144844 A CN1144844 A CN 1144844A
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China
Prior art keywords
rich
ferrum
spirulina platensis
production method
iron
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Application number
CN95109095A
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Chinese (zh)
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CN1063786C (en
Inventor
王敏
胡鸿均
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Wuhan Botanical Garden of CAS
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Wuhan Institute of Botany of CAS
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Priority to CN95109095A priority Critical patent/CN1063786C/en
Publication of CN1144844A publication Critical patent/CN1144844A/en
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Publication of CN1063786C publication Critical patent/CN1063786C/en
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Expired - Fee Related legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

A method for producing iron-enriched amblyspirulina, which can be used to prevent and cure iron-deficiency anemia, features that amblysirulina is cultured in an invented medium at 30-38 deg.C and 2400-2600 LX for 12 hr and the ferrous methionine in the medium is easily sucked to make iron content in amblyspriulina up to 7000 ug/g (dried weight). Its advantages are short production period and low cost.

Description

The production method of Spirulina Platensis rich in Ferrum
The present invention relates to the artificial culture of algae, particularly relate to the production method of a kind of spirulina plalensis (Spirulina Platensis) enrichment iron.
Spirulina is a kind of alkaline waters that is grown in, and form such as spiral helicine phycophyta belong to Cyanophyta on taxonomy, Cyanophyceae, hormogon[ium order, a genus---the Spirullina in the Oscillariaceae.Spirulina plalensis is a kind of Spirullina.
Spirulina contains rich nutrient substances, and wherein protein content is up to 60-70%, and amino acid whose composition is very reasonable in the protein, has necessary eight seed amino acids of human body.In addition, also be rich in multivitamin, mineral substance, chlorophyll, carotene, unsaturated fatty acids and polysaccharose substance, and nontoxic easy digestion, therefore be described as human classic food, have the wide development prospect.
In the contained mineral substance of spirulina, the necessary iron of human body (Fe) is arranged, the content of calcium (Ca), phosphorus (P) and zinc (Zn), particularly iron, iron content 600ug in every gram dry algae powder, the spirulina iron-holder of utilizing the Zarrouk substratum to produce is 800-1000ug/g (dry weight).See for details
Zarrouk?C，1966，Contribution ǎí?etude?dúnecyaniophcée?influence?de?divens?facteurs?Physiques?etchimiques?sur?la，Croissance?et?la?Photosynthese?deSpirulina?maxima(Setch.et?Gardner)Geitler?Ph.D.?thesis，University?of?Pairs，France。
U.S. Earthrise Farms company increases in the Zarrouk substratum for iron, and producing the spirulina powder iron-holder is 1500ug/g (dry weight).See for details
Robent?Henrikson，1989.Earth?Food?Spirulina.P.36.Ronere?Enterprises.Inc.
The iron-holder of the algae powder that the method for above-mentioned document introduction is produced, production efficiency and production cycle still are difficult to reach the requirement that rich iron algae powder is produced in industrialization.
The human body iron deficiency can cause iron deficiency anemia, and this disease is very general in China women, children and the elderly, and the medicine commonly used for the treatment of this disease at present is oral ferrous sulfate, can cause gastrointestinal side effect but take medicine for a long time, and absorption rate is low.
The objective of the invention is to propose a kind of novel technical method of preventing and treating the biological medicine source of iron deficiency anemia.Promptly produce the spirulina plalensis algae powder of Fu Tie,, and treat this sick biomedicinal raw material for the protective foods and the beverage of manufacturer production at iron deficiency anemia.
For achieving the above object, its technical scheme is:
Spirulina plalensis is seeded in the substratum, and when the OD value reaches 1.0, pH value is 10.5 o'clock, add a certain amount of easy absorbed ferrous methionine, at low light level 2400-2600Lx, cultivated 12 hours down, just can obtain the spirulina plalensis algae powder of iron-holder for 30-38 ℃ up to 7000ug/g (dry weight).
The present invention compared with prior art has following advantage and effect
1, is enriched in the interior iron level height of spirulina plalensis.High 7 times than with the ZShi culture medium culturing, than Earthrise Farms company produce high 4.6 times.
2, production cost reduces.The nutritive ingredient Fe-EDTA of substratum has reduced 3/4ths, NaNo 3Amount has reduced 2g/L.
3, cultivate save energy with the low light level.Incubation time shortens, only 12 hours production phase.
Realize best way of the present invention:
1, culture medium prescription (g/L)
NaHCO 3 16.8
NH 3H 2O: NaNO 3=1: 4 total nitrogen 100mgN/L
NaCl 1.0
K 2SO 4 1.0
KH 2PO 4 0.5
FeSO 4·7H 2O 0.0025
EDTA·Na 2 0.02
MgSO 4·7H 2O 0.2
CaCl 2·2H 2O 0.04
A 5 1ml
B 6 1ml
Distilled water 1000ml
PH 8.5-9
2, production stage: several steps in the following order carries out. Washing, drying---→ Spirulina Platensis rich in Ferrum algae powder.
Spirulina plalensis is seeded in stir culture in the substratum, reaches 1.0 when being cultured to the OD value, and pH value reaches at 10.5 o'clock, adds ferrous methionine in culture, and add-on is 1.08 * 10 -3Mol/l.
At temperature 30-38 ℃, continue to be cultured to 12 hours under the intensity of illumination 2400-2600LX and collect the algae powder, spray-dried, obtain the spirulina plalensis dry powder of rich iron, water content 5-7%.

Claims (6)

1, the production method of Spirulina Platensis rich in Ferrum is characterized in that used substratum is by N, P, C, K, Na, Mg, Ca, A 5, B 6, nutritive element such as EDTA-Fe forms, and in the training period, adds a certain amount of ferrous methionine in substratum, under optimal temperature and illumination condition,, regather, wash, the dry Spirulina Platensis rich in Ferrum dry algae powder that obtains through 12 hours cultivation.
2, the described Spirulina Platensis rich in Ferrum production method of claim 1 is characterized in that the composition content of substratum is (g/L)
NaHCO 3 16.8
NH 3H 2O: NaNO 3=1: 4 total nitrogen 100mgN/L
NaCl 1.0
K 2SO 4 1.0
KH 2PO 4 0.5
FeSO 4·7H 2O 0.0025
EDTA·Na 2 0.02
MgSO 4·7H 2O 0.2
CaCl 2·2H 20 0.04
A 5 1ml
B 6 1ml
Distilled water 1000ml
PH 8.5-9
3, Spirulina Platensis rich in Ferrum production method according to claim 1 is characterized in that in the process of cultivating, and when OD value reaches 1.0, pH value reaches at 10.5 o'clock, by adding ferrous methionine 1.08 * 10 in every liter of substratum -3Mol.
4, the production method of Spirulina Platensis rich in Ferrum according to claim 1 is characterized in that culture temperature is 30-38 ℃.
5, the production method of Spirulina Platensis rich in Ferrum according to claim 1 is characterized in that the intensity of illumination of cultivating is 2400-2600LX.
6, the production method of Spirulina Platensis rich in Ferrum according to claim 1 is characterized in that the water content of the Spirulina Platensis rich in Ferrum dry powder that obtains is 5-7%.
CN95109095A 1995-09-05 1995-09-05 Manufacturing method of iron-rich pure spirulina Expired - Fee Related CN1063786C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN95109095A CN1063786C (en) 1995-09-05 1995-09-05 Manufacturing method of iron-rich pure spirulina

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN95109095A CN1063786C (en) 1995-09-05 1995-09-05 Manufacturing method of iron-rich pure spirulina

Publications (2)

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CN1144844A true CN1144844A (en) 1997-03-12
CN1063786C CN1063786C (en) 2001-03-28

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102533557A (en) * 2010-12-22 2012-07-04 丽江程海保尔生物开发有限公司 Overwintering, rejuvenation and preservation culture medium for cultivating spirulina
CN102586491A (en) * 2012-03-02 2012-07-18 云南绿A生物工程有限公司 Method for controlling heavy metals in process of culturing spirulina
CN102793216A (en) * 2012-09-12 2012-11-28 北京林业大学 Method for preparing spiral seaweed powder by utilizing microwave vacuum freeze drying
CN102845781A (en) * 2012-09-01 2013-01-02 山东博然螺旋藻生物股份有限公司 Preparation method of chrome-rich instant spiral algae
CN104450581A (en) * 2014-12-10 2015-03-25 安徽大学 Cultivation method of hpyerglycemic spirulina
CN105639495A (en) * 2016-01-08 2016-06-08 烟台布鲁拜尔生物制药有限公司 Preparation method of high-iron spirulina powder
CN110004103A (en) * 2016-12-31 2019-07-12 北京林业大学 A method of regulation nutritious spirulina elemental iron element accumulation

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101492644B (en) * 2008-12-04 2013-06-05 上海化工研究院 Method for biosynthesis of 15N mark helix alga

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1087775C (en) * 1992-10-16 2002-07-17 中国农业科学院土壤肥料研究所 Tech. for culturing spirulina rich in selenium
CN1092104A (en) * 1994-01-30 1994-09-14 曹吉祥 The preparation method of germanium and selenium spirulina

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102533557A (en) * 2010-12-22 2012-07-04 丽江程海保尔生物开发有限公司 Overwintering, rejuvenation and preservation culture medium for cultivating spirulina
CN102533557B (en) * 2010-12-22 2014-09-10 丽江程海保尔生物开发有限公司 Overwintering, rejuvenation and preservation culture medium for cultivating spirulina
CN102586491A (en) * 2012-03-02 2012-07-18 云南绿A生物工程有限公司 Method for controlling heavy metals in process of culturing spirulina
CN102586491B (en) * 2012-03-02 2013-08-07 云南绿A生物工程有限公司 Method for controlling heavy metals in process of culturing spirulina
CN102845781A (en) * 2012-09-01 2013-01-02 山东博然螺旋藻生物股份有限公司 Preparation method of chrome-rich instant spiral algae
CN102845781B (en) * 2012-09-01 2014-05-14 山东博然螺旋藻生物股份有限公司 Preparation method of chrome-rich instant spiral algae
CN102793216A (en) * 2012-09-12 2012-11-28 北京林业大学 Method for preparing spiral seaweed powder by utilizing microwave vacuum freeze drying
CN104450581A (en) * 2014-12-10 2015-03-25 安徽大学 Cultivation method of hpyerglycemic spirulina
CN105639495A (en) * 2016-01-08 2016-06-08 烟台布鲁拜尔生物制药有限公司 Preparation method of high-iron spirulina powder
CN110004103A (en) * 2016-12-31 2019-07-12 北京林业大学 A method of regulation nutritious spirulina elemental iron element accumulation

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