CN1322464A

CN1322464A - Concentrated probiotics culture liquid for aquatic farming and its usage

Info

Publication number: CN1322464A
Application number: CN 01111415
Authority: CN
Inventors: 熊福祥; 邱国光; 林青; 吴捷光
Original assignee: 熊福祥
Priority date: 2001-03-06
Filing date: 2001-03-06
Publication date: 2001-11-21

Abstract

Concentrated culture liquid comprising protein, amino acid, sugar, phosphate and potassium salt solution is diluted with pond water in 20-200 times and inoculated with probiotics in 10-50% of culture of several days before being added to pond. The present invention makes probiotics be used to pond environment. The present invention can raise the yield and quality of aqueous product, especially high-grade one, at relatively lowr cost.

Description

The concentrated probiotics culture liquid and the using method thereof that are used for aquaculture

The present invention relates to culture fluid and the using method thereof of probio in the aquaculture, also be applicable to water microorganisms and planktonic cultivation.

Probio just is being widely used in the aquaculture, to the purifying aquatic product cultivating environment, improve fish, shrimp survival rate, control fish and shrimp diseases etc. and more and more play a significant role, be the upsurge of world today's research and application, using is at most photosynthetic bacteria the most widely, secondly is nitrifier.Photosynthetic bacteria can both utilize simple organic and H in little aerobic, anaerobism illumination, aerobic illumination, aerobic dark under the strictly anaerobic condition ₂Harmful substance such as S, ammonia nitrogen is bred growth rapidly as its nutrients.Therefore significant to purifying water, and probios such as photosynthetic bacteria itself are again the high-quality bait of fish, shrimp, growth promotion and disease prevention and cure to fish, shrimp play an important role, it is that pollutant decomposes and finally produces volatility ammonia discharge water body and water quality is purified rapidly etc. that nitrifier can make protein in the pond, the mechanism of action of probio and effect have obtained abundant confirmation in practice and more and more have been subjected to people's welcome, even are described as the wuwa of aquaculture.Obtained favorable economic benefit, social benefit and ecological benefits, but because water body is bigger in the aquaculture, need the amount of interpolation bigger, therefore cost is higher, and since in laboratory with favourable conditions or the probio that comes out of plant produced directly render in the pond, the process of an adaptation also arranged and weaken the competition survival ability of probio in the pond, and make the lability that shows result of use in the practice.

The objective of the invention is to invent a kind of broad spectrum activity probiotic's culture liquid, be suitable for the cultivation of various probios, and concentrating its whole nutrition only need to make it water with the pond of aquaculture to dilute can to obtain culture fluid completely, like this under the effect of the probio that inoculates high concentration, guarantee the absolute predominance of probio and guarantee its growth and breeding, and the water of culture fluid is taken from the pond and is made probio that a good procedure of adaptation be arranged, can adapt to rapidly after dropping into the pond, can reduce cost again, can need the plan preparation according to production again uses, quality of guaranteeing supply and quantity make the bigger effect of the real performance of probio.

The present invention realizes like this.

Although the kind of probio is a lot, nutritional requirement of cultivating and condition be difference to some extent also, but it is that all nutriments all must be to be decomposed into lower-molecular substance just to absorb that a common feature is arranged, its main component is N, P, K, and N: P: K is than having satisfied the needs of the probio of all uses at present 10: 0.5～1.5: 0.5～1.5.Other trace elements are owing to directly using the water in the pond by the time to solve, as long as so enough free amino acids, monose and phosphate anion, potassium ion are arranged in the culture fluid and satisfy the nutritional need that aforementioned proportion can satisfy various probios.As long as be concentrated to so to a certain degree and can form concentrated culture fluid, only need to be diluted to finite concentration during use and get final product with pond water.

Further set forth below in conjunction with embodiment.

Concentrate culture fluid and form, and egg dawn white matter content is 20～60% by protein, free radical acid, sugar, phosphate, the sylvite aqueous solution.Free aminoacid content 10～30%, N: P: the K ratio is 10: 0.5～1.5: 0.5～1.5, moisture is 25～50%, protein can be the water-soluble protein of any energy, preferably use peptone, the compound amino acid that free amino acid produces with compound amino acid or hydrolyzed protein, or the compounded amino acid product that after hydrolysis, obtains of the high protein product that after fermenting, generates with agricultural byproducts such as corn, dregs of beans, tapiocas, sugar is that sucrose or glucose or corn, tapioca etc. contain the sugar product that the agricultural byproducts of polysaccharide produce after hydrolysis.Can to contain agricultural byproducts such as sugar and protein be the raw material high protein that meets feeding standard that carries out producing in the fermentation production process with utilizing in sugar industry, food industry, pharmaceuticals industry, the chemical industry production process in order further to reduce cost also, contain sugar by product protein, free amino acid and sugar etc. and suitably add water-soluble phosphate or sylvite according to the content of its phosphorus, potassium and allocate and get final product after hydrolysis.If having met within the aforementioned proportion, P, K content promptly need not allocate.Phosphate is that soluble phosphate, sylvite such as calcium dihydrogen phosphate, potassium dihydrogen phosphate are to get final product with water-soluble sylvite such as potassium chloride.The using method that concentrates culture fluid is for directly with the concentrated culture fluid to 20 of pond water dilution～200 times, add probio liquid then and account for 10～50% of total amount, cultivating the back by the cultural method of this probio liquid adds in the pond, also can be if pond water is too dense with using after fine sand filtration treatment or the precipitation process, or with replacement of food running water or part replacement, to reduce inoculum concentration and the incubation time of probio.

Probio is the cultural method of photosynthetic bacteria: the culture fluid for preparing is packed in the overlay bag, and sealing sack, be positioned under the open-air sunlight and got final product in illumination cultivation 5-7 days, also can pack into molten device and to the bottom bubbling air, throughput is the air that per minute feeds culture fluid volume 50～200%, and illumination cultivation got final product in 1～3 day under the sunlight in the open.Or earlier in molten device the inflation illumination cultivation to move into 1～2 day effect of transparent plastic bag sealing strictly anaerobic illumination cultivation in 1～2 day then better.

Probio is aerobic type probio, and its cultural method is: the culture fluid for preparing is packed in the molten device to molten device bottom bubbling air, and throughput is the air that per minute feeds culture fluid volume 50～200%, and air feed is cultivated and got final product in 1～3 day continuously.Gas type probio is to adopt the interruption air feed to cultivate in the venting process to get final product if hold concurrently.

Use method of the present invention, the cost that makes probio make an addition to the pond reduces by 2～10 times than prior art, and result of use is reliable more, stable and assurance, make effect and the effect of probio really reach embodiment and extensive use, to improving the output of aquaculture, the breed of the significant particularly high-quality high-grade of quality aquatic products will be brought into play bigger benefit function.

Claims

1, the concentrated probiotics culture liquid that is used for aquaculture, it is characterized in that this concentrated culture fluid is made up of protein, free amino acid, sugar, phosphate, the sylvite aqueous solution, and gross protein value 20～60%, free aminoacid content 10～30%, N: p: the k ratio is 10: 0.5～1.5: 0.5～1.5, moisture 25～50%.

2, concentrated culture fluid according to claim 1, it is characterized in that directly dilution concentrates culture fluid to 20～200 times with pond water or with drinking water or through the pond water after fine sand filtration treatment or the precipitation process, add probio liquid then and account for 10～50% of total amount, cultivate the back by the cultural method of this probio liquid and add in the pond.

3, concentrated culture fluid according to claim 1, it is characterized in that described protein is peptone, free amino acid is compound amino acid or the compound amino acid of hydrolyzed protein generation or the free compounded amino acid product that obtains after hydrolysis with the high protein product of agricultural byproducts such as corn, dregs of beans, tapioca after fermentation, sugar is sucrose or glucose or corn, and tapioca etc. contain the sugar product that the polysaccharide agricultural byproducts produce after hydrolysis.

4, concentrated culture fluid according to claim 1, it is characterized in that containing agricultural byproducts such as sugar and protein and being the raw material high protein that meets feeding standard that carries out producing in the fermentation production process, containing the protein that sugar by product obtains after hydrolysis with utilizing in sugar industry, food industry, pharmaceuticals industry, the chemical industry production process, free amino acid and sugar etc. also suitably add water-soluble phosphate or sylvite gets final product according to the content of phosphorus potassium.

5, using method according to claim 2, it is characterized in that described probio is a photosynthetic bacteria, its cultural method is to pack into the culture fluid for preparing in the overlay bag and seal sack, be positioned over that illumination cultivation got final product in 5～7 days under the open-air sunlight, or in the molten device of packing into and to molten device bottom bubbling air, throughput is that per minute feeds the air of cultivating liquid 50～200%, and continuous air feed cultivation got final product in 1～3 day under the sunlight in the open.Or in molten device oxygenation illumination cultivation 1～2 day, move into transparent plastic bag sealing strictly anaerobic illumination cultivation then and got final product in 1～2 day.

6, method according to claim 2, it is characterized in that described probio is aerobic type probio, its cultural method is that the culture fluid for preparing is packed in the container to molten device bottom bubbling air, throughput is 50～200% air that per minute feeds the culture fluid volume, air feed is cultivated and was got final product in 1～3 day continuously, or the facultative type probio is to adopt the discontinuous air feed to cultivate in the venting process to get final product.