CN1823570A - Cultivation of selenium enriched edible fungus and method of producing natural selenium enriched milk using its tailing - Google Patents

Cultivation of selenium enriched edible fungus and method of producing natural selenium enriched milk using its tailing Download PDF

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CN1823570A
CN1823570A CNA2006100461484A CN200610046148A CN1823570A CN 1823570 A CN1823570 A CN 1823570A CN A2006100461484 A CNA2006100461484 A CN A2006100461484A CN 200610046148 A CN200610046148 A CN 200610046148A CN 1823570 A CN1823570 A CN 1823570A
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selenium
rich
mushroom
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enriched
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CN100358411C (en
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崔澎
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Liaoning Dongfang Biotechnology Research Institute
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
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    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

A method for culturing the Se-enriched edible fungus includes such steps as preparing culture medium from corn cob, straw powder, rice (or wheat) bran, gypsum, magnesium sulfate, zinc sulfate, Se salt and water, loading it in bogs, sealing, sterilizing, inoculating edible fungus spawn, fermenting, culturing mycelia, and spraying Se-enriched nutritive liquid. A method for producing Se-enriched milk by use of the leftover generated by culturing Se-enriched edible fungus features that the leftover is used as the additive of feed for quantitatively feeding the milk cow.

Description

The cultivation of selenium-enriched edible mushroom and offcuts thereof are produced the method for natural selenium enriched milk
Technical field
The present invention relates to the cultivation of selenium-enriched edible mushroom and the method that offcuts-Fu selenium edible fungi residue feed is produced natural selenium enriched milk thereof.
Background technology
The meeting of the United Nations's health organization Beijing in 1996 is announced: " selenium is the trace element of needed by human." there is the not enough problem of selenium intake in various degree in the population of China 2/3rds, per day for adults intake 40000-60000 nanogram selenium is recommended by China NI and the every day selenium intake of generally being grown up only is about 26600 nanograms.More existing animals and plants levels are difficult to satisfy the requirement of human body to selenium.
Selenium is one of 14 kinds of trace elements of needed by human, and it has the oxidation of the cells in vivo of inhibition, enhancing body immunity, the anti-ageing effect of waiting for a long time.Scarce selenium can bring out angiocardiopathy, tumour, Keshan disease etc., and to take the photograph the selenium amount too much, and selenosis takes place easily.The nutritionist points out that except that dietary fiber, milk contains the complete nutrients matter of needed by human body, and an adult drinks 500ml milk every day, can obtain 15~17g high-quality protein; Can obtain 600mg calcium, be equivalent to 80% of daily requirement; Can satisfy 11% of human body amphemera requirement.But the content of selenium is lower than 5 μ g/g in the milk, (it is a kind of offcuts of producing edible mushroom to the rich selenium edible fungi residue feed that we very easily absorb by adding ox body in feed, selenium content 160-240mg/g), transform the milk that is produced through body, selenium content is abundant, meet in the state food selenium sanitary standard (GB13105-91) of limiting the quantity of, this is referred to as Bioconverted selenium-enriched milk, rather than in milk, adding the condensed food of selenium preparation. Bioconverted selenium-enriched milk is to mend the fabulous functional drink that the strong body of selenium is achieved many things at one stroke, and has the excellent development prospect.
Summary of the invention
One of purpose of the present invention provides a kind of cultivation method of selenium-enriched edible mushroom.
Two of purpose of the present invention provides the method that a kind of offcuts with the cultivation of selenium-rich edible mushroom are produced rich selenium edible fungi residue feed.
Three of purpose of the present invention provides a kind of method of producing natural selenium enriched milk.
The cultivation method of selenium-enriched edible mushroom is characterized in that the selenium-enriched edible mushroom raw material makes by weight ratio by following composition: corncob 30-50 part, powder of straw 20-40 part, rice bran or wheat bran 10-30 part, gypsum 1-2 part, magnesium sulfate 0.5-1 part, zinc sulphate 0.2-0.4 part, lime 1-2 part, salt 1-2 part, potassium dihydrogen phosphate 0.1-0.3 part, selenium salt 0.1-0.5 part, water 60-80 part; PH value 6.5-7, pack, seal, 100 ℃ of temperature condition of concora crush sterilization down kept 8-12 hour, or high pressure 0.15Mpa kept 2 hours under 127 ℃ of conditions of temperature, take the dish out of the pot when being cooled to below 30 ℃, under aseptic condition, insert edible bacterium, place then and send out the fermentation of bacterium chamber, temperature is controlled at 22-25 ℃, humidity is controlled at 60%--70%, sends out the bacterium time at 20-40 days, and mycelia is covered with whole charge level, promptly make selenium-enriched edible mushroom bacterium rod, the bacterium rod enters the mushroom producing room management of producing mushroom, and temperature is 5 ℃-25 ℃, needs the stimulation at 80-90% of 5 ℃-10 ℃ cooling and humidification humidity then, spray selenium-rich nutritive liquid simultaneously, selenium-rich nutritive liquid addition is that 1Kg bacterium rod adds 20ml selenium-rich nutritive liquid, just visible mushroom flower bud, and general bacterium rod can go out 4-6 tide mushroom; The constituent of described selenium-rich nutritive liquid is by weight ratio: 1000 parts in the fertile 0.2-0.4 parts of No. 2 rich selenium of glucose 10-15 part, Shen rich board bio-azotobacter fertilizer 80-100 part, 6-BA 0.1-0.2 part, green road board, water, the bright product selenium content of the edible mushroom of production is 180-280mg/g.
Described selenium salt is meant sodium selenate, a kind of in sodium selenite, the calcium selenate.
Described edible mushroom, comprise: kinds such as Pleurotus class flat mushroom, phoenix-tail mushroom, Ji mushroom, Pleuotus nebrodensis Quel, Xingbao mushroom, Pleurotus citrinopileatus, Asparagus, Agaricus Bisporus, Stropharia rugoso-annulata, Agaricus blazei Murrill, woodear, Uricularia polytricha, white fungus, mushroom, coprinus comatus, straw mushroom, Hypsizygus marmoreus, Hericium erinaceus, glossy ganoderma, Cordyceps sinensis, Cordyceps militaris, grifola frondosus etc. have realized tame kind.
Produce the method for rich selenium edible fungi residue feed with the offcuts of cultivation of selenium-rich edible mushroom, it is characterized in that: pollution-free with behind the above-mentioned fruiting, no insect pest selenium-enriched edible mushroom bacterium rod, be displaced to and be exposed to the sun under the sunlight 3-5 days, organic selenium content in the test bacterium rod reaches 160-240mg/kg and is advisable, with sack filling machine that the bacterium rod is broken, add the A Musi biological feedstuff transforming agent of percentage by weight 0.2%--0.4% and the rice bran or the wheat bran of percentage by weight 20%, if during Se content deficiency 160-240mg/g, need add percentage by weight in addition is the selenium salting liquid of 0.1-0.4%, heap fermentation: one time windrow is no less than 4 cubic metres, height is more than 1 meter, and environmental temperature is more than 20 ℃; Begin overturning during heap temperature rise to 50 ℃, once a day, as pile temperature and surpass 65 ℃, add time overturning again; Fermentation time is 5 days; The sign that edible fungi residue feed ferments: the heap temperature drop is low, and material is loose, and the former stink of no material has the ammonia flavor slightly, is covered with white hypha in the heap; Carry out the semi-finished product oven dry then: bake out temperature is 50-70 ℃, also can dry naturally; Powder particle diameter is at 0.3-0.6mm; Quality inspection: to reach 160-240mg/g be qualified products to organic selenium content in the edible fungi residue feed.
Method with above-mentioned rich selenium edible fungi residue feed production natural selenium enriched milk, it is characterized in that: under the normal feed condition of dairy farm, add and feed rich selenium edible fungi residue feed, quantitative feeding, every ox adds hello above-mentioned rich selenium edible fungi residue feed 5kg-10kg every day, and it is good taking in the 20-40mg organic selenium with every ox day.
Advantage of the present invention is: technology is simple, easy to operate, when producing selenium-enriched edible mushroom, utilize its offcuts to produce rich selenium edible fungi residue feed, the selenium content of major product (edible mushroom, milk) and rich product (edible fungi residue feed) is all high, Se-enriched feedstuff has animal builds up resistance and immunity, prevents the generation of various Animal diseases, feeds pigs, fowl poultry kind effect such as sheep, horse, chicken, duck, goose is all good.
Embodiment
Embodiment 1:
The cultivation method of selenium-enriched edible mushroom, the selenium-enriched edible mushroom raw material is made by weight ratio by following composition: 40 parts of corncobs, 30 parts of powder of straw, rice bran or 20 parts in wheat bran, 1 part in gypsum, 0.5 part in magnesium sulfate, 0.2 part in zinc sulphate, 1 part of lime, 1 part of salt, 0.1 part of potassium dihydrogen phosphate, 0.1 part of selenium salt, 70 parts in water; PH value 6.5, pack, seal, place under 100 ℃ of temperature condition of concora crush autoclave to keep 8-12 hour, or high pressure 0.15Mpa kept 2 hours under 127 ℃ of conditions of temperature, take the dish out of the pot when being cooled to below 30 ℃, under aseptic condition, insert edible bacterium, place then and send out the fermentation of bacterium chamber, temperature is controlled at 22-25 ℃, humidity is controlled at 60%--70%, sends out the bacterium time at 20-40 days, and mycelia is covered with whole charge level, promptly make selenium-enriched edible mushroom bacterium rod, the bacterium rod enters the mushroom producing room management of producing mushroom, and temperature is 5 ℃-25 ℃, needs the stimulation at 80-90% of 5 ℃-10 ℃ cooling and humidification humidity then, spray selenium-rich nutritive liquid simultaneously, selenium-rich nutritive liquid addition is that 1Kg bacterium rod adds 20ml selenium-rich nutritive liquid, just visible mushroom flower bud, and general bacterium rod can go out 4-6 tide mushroom; The constituent of described selenium-rich nutritive liquid is by weight ratio: 12 parts of glucose, 90 parts of the rich board bio-azotobacter fertilizers in Shen, 0.1 part of 6-BA, fertile 0.2 part of No. 2 rich selenium of green road board, 1000 parts in water.
Described selenium salt is meant sodium selenate, a kind of in sodium selenite, the calcium selenate.
Described edible mushroom, comprise: Pleurotus class flat mushroom, phoenix-tail mushroom, the kinds such as Ji mushroom, Pleuotus nebrodensis Quel, Xingbao mushroom, Pleurotus citrinopileatus of beating a drum, Asparagus, Agaricus blazei Murrill, Agaricus Bisporus, Stropharia rugoso-annulata, woodear, white fungus, mushroom, coprinus comatus, straw mushroom, Hypsizygus marmoreus, Hericium erinaceus, glossy ganoderma, Cordyceps sinensis, Cordyceps militaris, grifola frondosus etc. have realized tame kind.
Produce the method for rich selenium edible fungi residue feed with the offcuts of cultivation of selenium-rich edible mushroom, it is characterized in that: pollution-free with behind the above-mentioned fruiting, no insect pest selenium-enriched edible mushroom bacterium rod, be displaced to and be exposed to the sun under the sunlight 3-5 days, organic selenium content in the test bacterium rod reaches 160-240mg/g and is advisable, with sack filling machine that the bacterium rod is broken, add the A Musi biological feedstuff transforming agent of percentage by weight 0.2%--0.4% and the rice bran or the wheat bran of percentage by weight 20%, if during Se content deficiency 160-240mg/g, need add percentage by weight in addition is the selenium salting liquid of 0.1-0.4%, heap fermentation: one time windrow is no less than 4 sides, height is more than 1 meter, and environmental temperature is more than 20 ℃; Begin overturning during heap temperature rise to 50 ℃, once a day, as pile temperature and surpass 65 ℃, add time overturning again; Fermentation time is 5 days; The sign that edible fungi residue feed ferments: the heap temperature drop is low, and material is loose, and the former stink of no material has the ammonia flavor slightly, is covered with white hypha in the heap; Carry out the semi-finished product oven dry then: bake out temperature is 50-70 ℃, also can dry naturally; Powder particle diameter is at 0.3-0.6mm; Quality inspection: to reach 160-240mg/g be qualified products to organic selenium content in the edible fungi residue feed.
Method with above-mentioned rich selenium edible fungi residue feed production natural selenium enriched milk, it is characterized in that: under the normal feed condition of dairy farm, add and feed rich selenium edible fungi residue feed, quantitative feeding, every ox adds hello above-mentioned rich selenium edible fungi residue feed 5kg-10kg every day, and it is good taking in the 20-40mg organic selenium with every ox day.
Embodiment 2:
The cultivation method of selenium-enriched edible mushroom, its raw material is made by weight ratio by following compositions: 30 parts of corncobs, 20 parts of powder of straw, rice bran or 10 parts in wheat bran, 1 part in gypsum, 0.5 part in magnesium sulfate, 0.2 part in zinc sulphate, 1 part of lime, 1 part of salt, 0.1 part of potassium dihydrogen phosphate, 0.1 part of selenium salt, 60 parts in water; PH value 6.5, pack, seal, 100 ℃ of temperature condition of concora crush sterilization down kept 8-12 hour, or high pressure 0.15Mpa kept 2 hours under 127 ℃ of conditions of temperature, take the dish out of the pot when being cooled to below 30 ℃, under aseptic condition, insert edible bacterium, place then and send out the fermentation of bacterium chamber, temperature is controlled at 22-25 ℃, humidity is controlled at 60%--70%, sends out the bacterium time at 20--40 days, and mycelia is covered with whole charge level, promptly make selenium-enriched edible mushroom bacterium rod, the bacterium rod enters the mushroom producing room management of producing mushroom, and temperature is 5 ℃-25 ℃, needs the stimulation at 80-90% of 5 ℃-10 ℃ cooling and humidification humidity then, spray selenium-rich nutritive liquid simultaneously, selenium-rich nutritive liquid addition is that 1Kg bacterium rod adds 20ml selenium-rich nutritive liquid, just visible mushroom flower bud, and general bacterium rod can go out 4-6 tide mushroom; The constituent of described selenium-rich nutritive liquid is by weight ratio: glucose 10-15 part, 80 parts of the rich board bio-azotobacter fertilizers in Shen, 0.1 part of 6-BA, fertile 0.2 part of No. 2 rich selenium of green road board, 1000 parts in water.
The method of producing rich selenium edible fungi residue feed with the offcuts of described selenium-enriched edible mushroom is with embodiment 1.
Produce the method for natural selenium enriched milk with embodiment 1 with described rich selenium edible fungi residue feed.
Embodiment 3:
The cultivation method of selenium-enriched edible mushroom, its raw material is made by weight ratio by following compositions: 50 parts of corncobs, 40 parts of powder of straw, rice bran or 30 parts in wheat bran, 2 parts in gypsum, 1 part in magnesium sulfate, 0.4 part in zinc sulphate, 2 parts of limes, 2 parts of salt, 0.3 part of potassium dihydrogen phosphate, 0.5 part of selenium salt, 80 parts in water; PH value 7, pack, seal, 100 ℃ of temperature condition of concora crush sterilization down kept 8-12 hour, or high pressure 0.15Mpa kept 2 hours under 127 ℃ of conditions of temperature, take the dish out of the pot when being cooled to below 30 ℃, under aseptic condition, insert edible bacterium, place then and send out the fermentation of bacterium chamber, temperature is controlled at 22-25 ℃, humidity is controlled at 60%--70%, sends out the bacterium time at 20-40 days, and mycelia is covered with whole charge level, promptly make selenium-enriched edible mushroom bacterium rod, the bacterium rod enters the mushroom producing room management of producing mushroom, and temperature is 5 ℃-25 ℃, needs the stimulation at 80-90% of 5 ℃-10 ℃ cooling and humidification humidity then, spray selenium-rich nutritive liquid simultaneously, selenium-rich nutritive liquid addition is that 1Kg bacterium rod adds 20ml selenium-rich nutritive liquid, just visible mushroom flower bud, and general bacterium rod can go out 4-6 tide mushroom; The constituent of described selenium-rich nutritive liquid is by weight ratio: 15 parts of glucose, 100 parts of the rich board bio-azotobacter fertilizers in Shen, 0.2 part of 6-BA, fertile 0.4 part of No. 2 rich selenium of green road board, 1000 parts in water.
The method of producing rich selenium edible fungi residue feed with the offcuts of described selenium-enriched edible mushroom is with embodiment 1.
Produce the method for natural selenium enriched milk with embodiment 1 with described rich selenium edible fungi residue feed.

Claims (3)

1, the cultivation method of selenium-enriched edible mushroom is characterized in that its raw material made by weight ratio by following compositions: corncob 30-50 part, powder of straw 20-40 part, rice bran or wheat bran 10-30 part, gypsum 1-2 part, magnesium sulfate 0.5-1 part, zinc sulphate 0.2-0.4 part, lime 1-2 part, salt 1-2 part, potassium dihydrogen phosphate 0.1-0.3 part, selenium salt 0.1-0.5 part, water 60-80 part; PH value 6.5-7, pack, seal, 100 ℃ of temperature condition of concora crush sterilization down kept 8-12 hour, or high pressure 0.15Mpa kept 2 hours under 127 ℃ of conditions of temperature, take the dish out of the pot when being cooled to below 30 ℃, under aseptic condition, insert edible bacterium, place then and send out the fermentation of bacterium chamber, temperature is controlled at 22-25 ℃, humidity is controlled at 60%-70%, sends out the bacterium time at 20-40 days, and mycelia is covered with whole charge level, promptly make selenium-enriched edible mushroom bacterium rod, the bacterium rod enters the mushroom producing room management of producing mushroom, and temperature is 5 ℃-25 ℃, needs the stimulation at 80-90% of 5 ℃-10 ℃ cooling and humidification humidity then, spray selenium-rich nutritive liquid simultaneously, selenium-rich nutritive liquid addition is that 1Kg bacterium rod adds 20ml selenium-rich nutritive liquid, just visible mushroom flower bud, and general bacterium rod can go out 4-6 tide mushroom; The constituent of described selenium-rich nutritive liquid is by weight ratio: 1000 parts in the fertile 0.2-0.4 parts of No. 2 rich selenium of glucose 10-15 part, Shen rich board bio-azotobacter fertilizer 80-100 part, 6-BA 0.1-0.2 part, green road board, water.
2, produce the method for rich selenium edible fungi residue feed with the offcuts of the described selenium-enriched edible mushroom of claim 1, it is characterized in that: pollution-free with behind the above-mentioned fruiting, no insect pest selenium-enriched edible mushroom bacterium rod, be displaced to and be exposed to the sun under the sunlight 3-5 days, organic selenium content in the test bacterium rod reaches 160-240mg/kg and is advisable, with sack filling machine that the bacterium rod is broken, add the A Musi biological feedstuff transforming agent of percentage by weight 0.2%--0.4% and the rice bran or the wheat bran of percentage by weight 20%, if during Se content deficiency 160-240mg/kg, need add percentage by weight in addition is the selenium salting liquid of 0.1-0.4%, heap fermentation: one time windrow is no less than 4 sides, height is more than 1 meter, and environmental temperature is more than 20 ℃; Begin overturning during heap temperature rise to 50 ℃, once a day, as pile temperature and surpass 65 ℃, add time overturning again; Fermentation time is 5 days; The sign that edible fungi residue feed ferments: the heap temperature drop is low, and material is loose, and the former stink of no material has the ammonia flavor slightly, is covered with white hypha in the heap; Carry out the semi-finished product oven dry then: bake out temperature is 50-70 ℃, also can dry naturally; Powder particle diameter is at 0.3-0.6mm; Quality inspection: to reach 160-240mg/kg be qualified products to organic selenium content in the edible fungi residue feed.
3, the method for producing natural selenium enriched milk with the described rich selenium edible fungi residue feed of claim 2, it is characterized in that: under the normal feed condition of dairy farm, add and feed rich selenium edible fungi residue feed, want quantitative feeding, every ox adds hello above-mentioned rich selenium edible fungi residue feed 5kg-10kg every day, and it is good taking in the 20-40mg organic selenium with every ox day.
CNB2006100461484A 2006-03-24 2006-03-24 Cultivation of selenium enriched edible fungus and method of producing natural selenium enriched milk using its tailing Expired - Fee Related CN100358411C (en)

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CN101637101B (en) * 2009-08-19 2011-01-12 蛟河市黑土白云食用菌有限公司 Industrial cultivation method of selenium-enriched agaricus bisporus
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