CN105724323A - Selenium-rich tussah pupas and stocking method thereof - Google Patents
Selenium-rich tussah pupas and stocking method thereof Download PDFInfo
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- CN105724323A CN105724323A CN201610139565.7A CN201610139565A CN105724323A CN 105724323 A CN105724323 A CN 105724323A CN 201610139565 A CN201610139565 A CN 201610139565A CN 105724323 A CN105724323 A CN 105724323A
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- selenium
- pernyi
- meneville
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- 239000011669 selenium Substances 0.000 title claims abstract description 120
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 70
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 43
- 241000382353 Pupa Species 0.000 title abstract description 11
- 238000005507 spraying Methods 0.000 claims abstract description 54
- 241000255789 Bombyx mori Species 0.000 claims description 49
- 239000007921 spray Substances 0.000 claims description 28
- 230000005059 dormancy Effects 0.000 claims description 20
- 241000255978 Antheraea pernyi Species 0.000 claims description 17
- 238000009366 sericulture Methods 0.000 claims description 16
- 238000009941 weaving Methods 0.000 claims description 16
- 238000005516 engineering process Methods 0.000 abstract description 24
- 230000012010 growth Effects 0.000 abstract description 3
- UGSPLKDPMABWOB-UHFFFAOYSA-N selenium;hydrate Chemical compound O.[Se] UGSPLKDPMABWOB-UHFFFAOYSA-N 0.000 abstract 3
- 230000001418 larval effect Effects 0.000 abstract 2
- 241000219492 Quercus Species 0.000 abstract 1
- 230000002411 adverse Effects 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 238000009987 spinning Methods 0.000 abstract 1
- 229940091258 selenium supplement Drugs 0.000 description 57
- 230000000384 rearing effect Effects 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 14
- 238000001514 detection method Methods 0.000 description 14
- 239000000047 product Substances 0.000 description 7
- 230000009571 larval growth Effects 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 3
- 206010067482 No adverse event Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 206010058314 Dysplasia Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical group OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 235000003969 glutathione Nutrition 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 150000003342 selenium Chemical class 0.000 description 1
- 125000003748 selenium group Chemical group *[Se]* 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Materials For Medical Uses (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to selenium-rich tussah pupas and a stocking method thereof. The method includes the steps that selenium hydrate is sprayed to tussah breeding oak trees in the larval stage of tussah to obtain the selenium-rich tussah pupas, and the selenium content of the tussah pupas is 0.04-0.30 mg/kg. The concrete stocking technology includes the following steps that 1, the selenium hydrate is sprayed once or twice in the larval stage (1-5 age) of tussah, the selenium content is 2-500 mg/kg, the spraying dosage is 500-1000 ml, and the time periods like a rest stage, rainy days, noon (10:00-14:00) of sunny days are avoided when the selenium hydrate is sprayed; 2, after tussah pupa larvas complete 5-age growth, the tussah pupas are formed by spinning, cocooning and pupating. The selenium content in the tussah pupas can be effectively controlled, and the whole cocoon rate, pupa weight, cocoon shell amount and the cocoon shell rate are not affected adversely.
Description
Technical field
The present invention relates to a kind of selenium-rich nutritive insecticide product and production method thereof, be specifically related to one
Selenium-rich Antheraea pernyi Geurin Meneville and the stocking method of selenium-rich Antherea pernyi Guerin-Meneville.
Background technology
Selenium is trace element necessary to humans and animals, and human life activity plays important work
With, it is multiple enzyme, such as: the important composition composition of glutathion peroxidase (GDH-PX) etc..
People can reach to improve immunity of organisms by selenium supplement, improves resistance against diseases, slow down aging
Purpose.
Whole world selenium resource distribution is the most unbalanced, and China is also such.The ground selenium moneys such as enshi
Source is the abundantest, and China from northeast to one strip in southwest (accounting for China's area 70%) selenium
Resource feedback is weary, and indivedual areas (Keshan County, Heilungkiang) selenium resource is seriously presented weary.For meeting human body
The demand of normal selenium element, people investigated selenium-rich product miscellaneous.But, existing
Selenium-rich product is mainly selenium-rich agricultural byproducts, and its production method is mainly by applying sodium selenite
Etc. inorganic selenium salt or apply organic selenium fertilization, produce containing modes such as selenium composite fertilizers.
What Antherea pernyi Guerin-Meneville originated from China existing more than 3000 year puts history in a suitable place to breed, and the tussah cocoon of China produces
Amount accounts for the 80% of Gross World Product.The most just there is the custom of edible Antheraea pernyi Geurin Meneville in China.Antherea pernyi Guerin-Meneville
Have preferable accumulation ability to selenium, people can increase human body pair by edible selenium-rich Antheraea pernyi Geurin Meneville
The demand of selenium.But, use which kind of method in the case of ensureing Antherea pernyi Guerin-Meneville normal growth, it to be entered
Row is selenium nutrition-intensified is to need further investigation.
Summary of the invention
For solving above-mentioned technical problem, the invention provides a kind of selenium-rich Antheraea pernyi Geurin Meneville and selenium-rich Antherea pernyi Guerin-Meneville
Stocking method.Wherein, described selenium-rich Antheraea pernyi Geurin Meneville is obtained by following stocking method, its toothed oak
In Pupa bombycis, Se content is 0.04～0.30mg/kg, and described stocking method comprises the following steps:
(1) in pernyi larvae 1-5 age, to the hydrate of sericulture Querci Mongolicae Spraying Se, selenium
Concentration is 2-500mg/L, sprays 1-2 time full age, and solution dosage used is 500ml-1000ml；
Dormancy phase, rainy day, fine day high noon is avoided when spraying；
(2) treat pernyi larvae complete 5 age process, to weave silk cocoon pupates.
It is a further object of the present invention to provide the stocking method of a kind of selenium-rich Antherea pernyi Guerin-Meneville, including following step
Rapid:
(1) in the pernyi larvae phase (1-5 age), to the hydrate of sericulture Folium Querci Mongolicae Spraying Se
Thing, selenium concentration is 2-500mg/L, sprays 1-2 time full age, and solution dosage used is
500ml-1000ml；Avoid the period of spraying the dormancy phase, the rainy day, fine day high noon (10:00-14:
00)；
(2) treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, and i.e. obtains selenium-rich toothed oak
Pupa bombycis.
Preferred as the present invention, described in use micro sprayer to spray when spraying.
As present invention further optimization, described in put period in a suitable place to breed, according to the Querci Mongolicae age of tree, tree crown
Size and Antherea pernyi Guerin-Meneville age, every strain Querci Mongolicae puts Antherea pernyi Guerin-Meneville 300-50 in a suitable place to breed, tussah silkworm nursery temperature, wet
Degree, illumination nature.
Of the present invention avoid the dormancy phase, i.e. general molting together after within the 3rd day, uses；Described keeps away
Opening the rainy day refers to that spraying before and after avoided raining within 5 hours；Described avoid fine day high noon one
As refer to, between 10:00-14:00, not spray.
Toothed oak young silkworm hatching date: according to different geographical climatic characteristic, univoltine area is general 4
On 5-April 15 moon, it is general in 5-July 20 July that area is put in two changes one, and two change
Area silkworm in spring is typically the 26-5 month in April 5, and autumn silkworm is typically in 25-August 5 July.
Advantage of the present invention and beneficial effect:
The concentration of Spraying Se hydrate when (1) the method for the invention is put in a suitable place to breed by control,
Dosage and spray period, it is possible to effectively control the content of selenium in Antheraea pernyi Geurin Meneville, and complete to Antherea pernyi Guerin-Meneville
Cocoon amount, pupal weight, cocoon shell weight, Cocoon layer ratio etc. all have no adverse effects.
(2) the method for the invention to univoltine Antherea pernyi Guerin-Meneville, two change one and put Antherea pernyi Guerin-Meneville and two and change and two put
Silkworm in spring, autumn silkworm all can guarantee that produces qualified selenium-rich Antheraea pernyi Geurin Meneville.
Detailed description of the invention
Embodiment 1
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1,5 age, to supporting
The hydrate of silkworm Querci Mongolicae Spraying Se, selenium concentration is 2mg/L, and sprayed dose is 500ml, spray
Executing number of times is 2 times；Wherein, age pernyi larvae phase 1 sprays 150ml；The pernyi larvae phase 5
Age sprays 350ml.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day
High noon (10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates,
Detection tussah silkworm chrysalis body Se content.
Embodiment 2
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1,5 age, to supporting
The hydrate of silkworm Querci Mongolicae Spraying Se, selenium concentration is 250mg/L, and sprayed dose is 700ml,
Spraying number of times is 2 times；Wherein, age pernyi larvae phase 1 sprays 250ml；The pernyi larvae phase
5 ages sprayed 450ml.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day
High noon (10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates,
Detection tussah silkworm chrysalis body Se content.
Embodiment 3
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1,5 age, to supporting
The hydrate of silkworm Querci Mongolicae Spraying Se, selenium concentration is 500mg/L, and sprayed dose is 1000ml,
Spraying number of times is 2 times；Wherein, age pernyi larvae phase 1 sprays 250ml；The pernyi larvae phase
5 ages sprayed 750ml.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day
High noon (10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates,
Detection tussah silkworm chrysalis body Se content.
Embodiment 4
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 100mg/L, and sprayed dose is 600ml, spray
Executing number of times is 1 time；Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day high noon
(10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detection
Tussah silkworm chrysalis body Se content.
Embodiment 5
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 2 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 200mg/L, and sprayed dose is 700ml, spray
Executing number of times is 1 time；Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day high noon
(10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detection
Tussah silkworm chrysalis body Se content.
Embodiment 6
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 3 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 300mg/L, and sprayed dose is 800ml, spray
Executing number of times is 1 time；Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day high noon
(10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detection
Tussah silkworm chrysalis body Se content.
Embodiment 7
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 4 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 400mg/L, and sprayed dose is 900ml, spray
Executing number of times is 1 time；Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day high noon
(10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detection
Tussah silkworm chrysalis body Se content.
Embodiment 8
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 5 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 500mg/L, and sprayed dose is 1000ml, spray
Executing number of times is 1 time；Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day high noon
(10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detection
Tussah silkworm chrysalis body Se content.
Comparative example 1
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1,5 age, to supporting
The hydrate of silkworm Querci Mongolicae Spraying Se, selenium concentration is 0.5mg/L, and sprayed dose is 1000ml,
Spraying number of times is 2 times；Wherein, age pernyi larvae phase 1 sprays 250ml；The pernyi larvae phase
5 ages sprayed 750ml.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day
High noon (10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates,
Detection tussah silkworm chrysalis body Se content.
Comparative example 2
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1,5 age, to supporting
The hydrate of silkworm Querci Mongolicae Spraying Se, selenium concentration is 0mg/L, and sprayed dose is 1000ml, spray
Executing number of times is 2 times；Wherein, age pernyi larvae phase 1 sprays 250ml；The pernyi larvae phase 5
Age sprays 750ml.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day
High noon (10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates,
Detection tussah silkworm chrysalis body Se content.
Comparative example 3
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1,5 age, to supporting
The hydrate of silkworm Querci Mongolicae Spraying Se, selenium concentration is 550mg/L, and sprayed dose is 500ml,
Spraying number of times is 2 times；Wherein, age pernyi larvae phase 1 sprays 150ml；The pernyi larvae phase
5 ages sprayed 350ml.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day
High noon (10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates,
Detection tussah silkworm chrysalis body Se content.
Comparative example 4
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 2 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 0.5mg/L, and sprayed dose is 1000ml, spray
Executing number of times is 1 time.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day high noon
(10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detection
Tussah silkworm chrysalis body Se content.
Comparative example 5
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 3 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 0mg/L, and sprayed dose is 1000ml, sprays
Number of times is 1 time.Use micro sprayer when spraying, avoid the dormancy phase, the rainy day, fine day high noon (10:
00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detect Antheraea pernyi Geurin Meneville
Body Se content.
Comparative example 6
Univoltine Antherea pernyi Guerin-Meneville, puts in a suitable place to breed and puts technology period etc. in a suitable place to breed with reference to conventional univoltine outdoor rearing of tussah silkworms skill
Art.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 4 age, to sericulture
The hydrate of Querci Mongolicae Spraying Se, selenium concentration is 550mg/L, and sprayed dose is 500ml, spray
Executing number of times is 1 time.Use micro sprayer when spraying, avoid dormancy phase, rainy day, fine day high noon
(10:00-14:00).Treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, detection
Tussah silkworm chrysalis body Se content.
Tussah silkworm chrysalis body Se content detects
Detection method: measure according to the method for standard GB/T 5009.93-2010 regulation.Adopt
By Hydride generation fluorescence spectrometry or fluorescence method.
Tussah silkworm chrysalis body Se content testing result is shown in Table 1:
Table 1 selenium-rich Antherea pernyi Guerin-Meneville economic indicator and pupal cell Se content testing result
When the present invention uses the stocking method of embodiment 1-8 and comparative example 1-6 to outdoor rearing of tussah silkworms,
The pernyi larvae that embodiment 1-8 is put in a suitable place to breed is all grown up healthy and sound, without sending out because of the hydrate of Spraying Se
Disease or death condition, and the selenium-rich Antherea pernyi Guerin-Meneville that Se content is 0.04～0.30mg/kg can be obtained
Pupa, and the cocoon weight of Antherea pernyi Guerin-Meneville, pupal weight, cocoon shell weight, Cocoon layer ratio etc. are all had no adverse effects.
The Antherea pernyi Guerin-Meneville that comparative example 1, comparative example 2, comparative example 4 and comparative example 5 are put in a suitable place to breed the most all can healthy one-tenth
Long, without morbidity or death condition, and to the cocoon weight of Antherea pernyi Guerin-Meneville, pupal weight, cocoon shell weight, cocoon
Layer rate etc. all has no adverse effects.But, the final Antheraea pernyi Geurin Meneville Se content obtained is relatively low, does not meets
Selenium-rich product requirement.Although the Antherea pernyi Guerin-Meneville that comparative example 3 and comparative example 6 are put in a suitable place to breed is to the final toothed oak obtained
Silkworm cocoon weight, pupal weight, cocoon shell weight, Cocoon layer ratio etc. all have negative effect in various degree,
The larva growth stage, also there is negative effect in various degree for indivedual pernyi larvae growth promoter,
, even there are the phenomena of mortality in some dysplasia.And the Antheraea pernyi Geurin Meneville Se content that obtains is higher,
Do not meet selenium-rich product requirement.
Embodiment 9-16
Two change and one put Antherea pernyi Guerin-Meneville, put in a suitable place to breed and put technology period etc. in a suitable place to breed and change one with reference to conventional two and put outdoor rearing of tussah silkworms
Technology.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1-5 age, to
The hydrate of sericulture Querci Mongolicae Spraying Se, concrete spraying method and the results are shown in Table 2.
Table 2 selenium-rich Antherea pernyi Guerin-Meneville application process, larval growth situation and pupal cell Se content result
Comparative example 7-12
Two change and one put Antherea pernyi Guerin-Meneville, put in a suitable place to breed and put technology period etc. in a suitable place to breed and change one with reference to conventional two and put outdoor rearing of tussah silkworms
Technology.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1-5 age, to
The hydrate of sericulture Querci Mongolicae Spraying Se, concrete spraying method and the results are shown in Table 3.
Table 3 selenium-rich Antherea pernyi Guerin-Meneville application process, larval growth situation and pupal cell Se content result
Embodiment 17-24
Two change and two put spring Antherea pernyi Guerin-Meneville, put in a suitable place to breed and put technology period etc. in a suitable place to breed and change two with reference to conventional two and put spring Antherea pernyi Guerin-Meneville
Put technology in a suitable place to breed.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1-5 age,
To the hydrate of sericulture Querci Mongolicae Spraying Se, concrete spraying method and the results are shown in Table 4.
Table 4 selenium-rich Antherea pernyi Guerin-Meneville application process, larval growth situation and pupal cell Se content result
Embodiment 25-32
Two change and two put autumn Antherea pernyi Guerin-Meneville, put in a suitable place to breed and put technology period etc. in a suitable place to breed and change two with reference to conventional two and put autumn Antherea pernyi Guerin-Meneville
Put technology in a suitable place to breed.Every strain Querci Mongolicae puts pernyi larvae 300-30 head in a suitable place to breed, in pernyi larvae 1-5 age,
To the hydrate of sericulture Querci Mongolicae Spraying Se, concrete spraying method and the results are shown in Table 5.
Table 5 selenium-rich Antherea pernyi Guerin-Meneville application process, larval growth situation and pupal cell Se content result
As can be seen here, for meeting the requirement of existing selenium-rich product, it is thus achieved that Se content be 0.04～
The selenium-rich Antheraea pernyi Geurin Meneville of 0.30mg/kg, ensures growing up healthy and sound, not to Antherea pernyi Guerin-Meneville of pernyi larvae simultaneously
Larva damages, and needs the strict hydrate concentration controlling selenium and sprayed dose.The present invention
Obtain selenium-rich Antheraea pernyi Geurin Meneville method described in embodiment to obtain through lot of experiments, to univoltine toothed oak
Silkworm, two changes one put Antherea pernyi Guerin-Meneville and silkworm in spring is put in two changes two, autumn silkworm all can guarantee that and produces qualified selenium-rich
Antheraea pernyi Geurin Meneville.Described embodiment is a part of embodiment of the present invention rather than whole enforcement
Example.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creativeness
The every other embodiment obtained under work premise, broadly falls into the scope of protection of the invention.
Claims (5)
1. a selenium-rich Antheraea pernyi Geurin Meneville, it is characterised in that: obtained by following stocking method, its
Antheraea pernyi Geurin Meneville Se content is 0.04～0.30mg/kg；Described stocking method comprises the following steps:
(1) in pernyi larvae 1-5 age, to the hydrate of sericulture Folium Querci Mongolicae Spraying Se,
Selenium concentration is 2-500mg/L, sprays 1-2 time full age, and solution dosage used is
500ml-1000ml；Dormancy phase, rainy day, fine day high noon is avoided when spraying；
(2) treat pernyi larvae complete 5 age process, to weave silk cocoon pupates.
2. the stocking method of a selenium-rich Antherea pernyi Guerin-Meneville, it is characterised in that comprise the following steps:
(1) in pernyi larvae 1-5 age, to the hydrate of sericulture Folium Querci Mongolicae Spraying Se,
Selenium concentration is 2-500mg/L, sprays 1-2 time full age, and solution dosage used is
500ml-1000ml；Dormancy phase, rainy day, fine day high noon is avoided when spraying；
(2) treat pernyi larvae complete 5 age process, weaving silk cocoons pupates, and i.e. can get selenium-rich
Antheraea pernyi Geurin Meneville.
The stocking method of a kind of selenium-rich Antherea pernyi Guerin-Meneville the most according to claim 2, its feature exists
In: the described dormancy phase of avoiding refers within the 3rd day, use after molting is together；The described rainy day of avoiding refers to
Spray before and after to avoid raining within 5 hours；Described fine day of avoiding refers to 10 high noon:
Do not spray between 00-14:00.
The stocking method of a kind of selenium-rich Antherea pernyi Guerin-Meneville the most according to claim 2, its feature exists
In: use micro sprayer to spray when spraying.
The stocking method of a kind of selenium-rich Antherea pernyi Guerin-Meneville the most according to claim 2, its feature exists
In: when putting in a suitable place to breed, according to the Querci Mongolicae age of tree, tree crown size and Antherea pernyi Guerin-Meneville age, every strain Querci Mongolicae puts toothed oak in a suitable place to breed
Silkworm 300-30.
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