CN102070250B - Photosynthetic bacterium water quality purifying agent and preparation method thereof - Google Patents
Photosynthetic bacterium water quality purifying agent and preparation method thereof Download PDFInfo
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- CN102070250B CN102070250B CN 200910194059 CN200910194059A CN102070250B CN 102070250 B CN102070250 B CN 102070250B CN 200910194059 CN200910194059 CN 200910194059 CN 200910194059 A CN200910194059 A CN 200910194059A CN 102070250 B CN102070250 B CN 102070250B
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Abstract
The invention relates to a photosynthetic bacterium water quality purifying agent and a preparation method thereof. The photosynthetic bacterium water quality purifying agent is mainly characterized by mainly comprising the following components in percentage by weight: 97 to 99.8 percent of photosynthetic bacterium liquid and 0.2 to 3 percent of calcium chloride, wherein the mixed liquid formed by mixing the components consists of supernatant and precipitation liquid. The preparation method is characterized by comprising the following steps of: 1) culturing photosynthetic bacteria, preparing photosynthetic bacterium liquid, measuring the photosynthetic bacterium liquid and pouring the measured photosynthetic bacterium liquid into a stirring container; 2) weighing and respectively pouring the calcium chloride, disodium hydrogen phosphate or sodium dihydrogen phosphate into the stirring container, and standing after stirring; and 3) standing until forming two layers, namely the supernatant and the precipitation liquid, to prepare the photosynthetic bacterium water quality purifying agent. The photosynthetic bacterium water quality purifying agent has low package and transportation cost, and the effects of purifying water quality and maintaining water quality ecological balance, improving the transparency of a water body and promoting the photosynthesis of algae.
Description
Technical field
The present invention relates to a kind of photosynthetic bacterium water quality cleansing agent and preparation method thereof, belong to the water body purification technical field.
Background technology
At present, along with the particularly development of aquaculture liquid of industrial and agricultural production, the problem of water quality deterioration becomes increasingly conspicuous.Improve water quality, the research of creating the water body environment of a suitable aquatic products thing growth also gets more and more.Photosynthetic bacterium has been proved to be in industries such as aquaculture, environment protection and has played important effect.Photosynthetic bacterium can be rested residual bait in the fish and water, movement etc. and decomposed fully, has the aquaculture of improvement ecotope, reduces ammonia nitrogen, BOD effect.As fish, shrimp, the crab open-mouthed bait of growing seedlings, can have additional nutrients in addition, improve surviving rate.Because of PSB is the aerobic bacterial classification mostly; In culturing the pond under the utilization of waterwheel or oxygenating device; Promote culturing pool to keep good fluidity, a large amount of oxygen is dissolved in the water, toxic gas is shed outside the water surface; In addition also can provide fish to obtain higher dissolved oxygen, also promote photosynthetic bacterium to the improvement of water quality, stablize effectiveness such as water colour, increase feed effect, the generation that wards off disease.In order to save packing and transportation cost, improve the cost performance of photosynthetic bacterium, various photosynthetic bacterium liquid concentrators have appearred on the market.Disclose a kind of preparation method of concentrating photosynthetic bacteria like the patent No. for the patent of invention of " 200510034501.2 ", utilized present method, can not influence under the active prerequisite of photosynthetic bacterium, on a large scale, simply, concentrating photosynthetic bacteria efficiently.The present invention realizes through following technical proposal; Utilize ordinary method to produce photosynthetic bacterium stoste; And carry out coarse filtration, and utilize ultrafiltration and concentration equipment circulating filtration, obtain 5 to 50 times photosynthetic bacterium enriching agent; Can increase substantially its cost performance, be more suitable for being used for industries such as aquaculture, livestock and poultry cultivation, plantation and environment protection.Also there is following defective in photosynthetic bacterium liquid concentrator of the prior art: it is few 1, to contain the photosynthetic bacteria cell number in the concentrating liquid, and the shelf time is short; 2, most photosynthetic bacterium all disperses in the pool water within, can not the material that become sour of bottom, pond effectively be decomposed.
Summary of the invention
First purpose of the present invention; Be to overcome the above-mentioned shortcoming that existing photosynthetic bacteria preparation exists; A kind of photosynthetic bacterium water quality cleansing agent is provided; It can make most photosynthetic bacterium be deposited at the bottom of the pond and the material that becomes sour of bottom, pond is effectively decomposed, and the cell count that has active photosynthetic bacterium simultaneously is many, the characteristics that the shelf time is long.
Second purpose of the present invention is for a kind of preparation method of photosynthetic bacterium water quality cleansing agent is provided.
First purpose of the present invention reaches through following measure:
A kind of photosynthetic bacterium water quality cleansing agent is characterized in: said water quality cleansing agent mainly is mixed and made into by photosynthetic bacterium liquid and calcium chloride, and hundred parts of content of the weight of each material composition are photosynthetic bacterium liquid 97%～99.8%, calcium chloride 0.2%～3%; The mixed solution that is mixed and made into is made up of supernatant and precipitated liquid.
First purpose of the present invention can also reach through taking following measure:
One embodiment of the present invention are: on the basis of photosynthetic bacterium liquid and calcium chloride, be added with Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC; Hundred parts of content of the weight of each material composition are: photosynthetic bacterium liquid 96%～99.8%; Calcium chloride 0.1%～2%, Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC 0.1%～2%.
One embodiment of the present invention are: hundred parts of content of the weight of each material composition are: photosynthetic bacterium liquid 99%, calcium chloride 0.5%, Sodium phosphate, dibasic 0.5%.
One embodiment of the present invention are: hundred parts of content of the weight of each material composition are: photosynthetic bacterium liquid 99%, calcium chloride 0.5%, SODIUM PHOSPHATE, MONOBASIC 0.5%.
Second purpose of the present invention reaches through following measure:
A kind of preparation method of photosynthetic bacterium water quality cleansing agent, its characteristics are may further comprise the steps:
1) adopts currently known methods of the prior art to cultivate photosynthetic bacterium, and be prepared into photosynthetic bacterium liquid, measure 1000kg photosynthetic bacterium liquid and pour in the stirred vessel;
2) take by weighing calcium chloride 5kg, Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC 5kg; They are poured into respectively in the stirred vessel, stirred 5 minutes, leave standstill;
3) leave standstill one hour after, when the solution in the stirred vessel forms supernatant and sedimentation liquid when two-layer; Promptly make the photosynthetic bacterium water quality cleansing agent;
Second purpose of the present invention can also reach through taking following measure:
One embodiment of the present invention are: in step 3), when the solution in the stirred vessel forms supernatant and sedimentation liquid when two-layer, with half extraction of supernatant, remainder is spissated photosynthetic bacterium water quality cleansing agent again.Help reducing packing and transportation cost.
One embodiment of the present invention are: the preparation method of described photosynthetic bacterium liquid carries out successively according to the following steps:
1) in agitation vat, pours the 500kg tap water into;
2) in agitation vat, add following material respectively: SODIUM PHOSPHATE, MONOBASIC 3000g, sodium-acetate 1100g, Hydrocerol A 800g, ammonium chloride 550g, sodium hydroxide 1100g, iron(ic)chloride 200g, calcium chloride 50g, yeast extract paste 55g;
3) above-mentioned substance was stirred in agitation vat 5 minutes, make it dissolving;
4) in agitation vat, add 300～500kg photosynthetic bacterium bacterial classification stoste, stirred 5 minutes, the liquid in the agitation vat pours in the cultivation pool then, guarantee abundant illumination condition under cultivate, incubation time is 7～10 days; To maintain the temperature at winter more than 20 ℃, maintain the temperature at summer below 35 ℃, when the color of solution becomes scarlet, cultivate completion, make photosynthetic bacterium liquid.
The invention has the beneficial effects as follows:
1, photosynthetic bacterium water quality cleansing agent of the present invention is mainly by photosynthetic bacterium liquid, calcium chloride, and Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC are mixed with and form; It is made up of supernatant and precipitated liquid; Earlier this scavenging agent is shaken up during use; And then pour in the fishpond, in a part of photosynthetic finely divided pool water within this scavenging agent Chi Shui being purified, most of photosynthetic bacterium is effectively decomposed the material that becomes sour at the bottom of being deposited to the pond.The cell count of this product unit weight has improved 4～7 times than like product, and the shelf time prolongs 2～4 times than like product, has packing, advantage that transportation cost is low.
2, purify water; Utilize hydrogen sulfide, ammonia nitrogen in the direct consume water of photosynthesis; And eliminate nitrite in the water quality etc. through denitrification; Make in the pond water quality and the pond at the bottom of residual bait, ight soil etc. decompose fully and absorb, thereby the organic content in the degradation water improves and reductions such as chemical oxygen amount and ammonia nitrogen sulfide oxygen in water.
3, the present invention is nutritious, and protein contnt is up to 66%, and contains 8 kinds of aquatic animal essential amino acids and abundant vitamin B group and the Serlabo of Gao Nongdu; Thalline contains carbon element stored substance sugar, ubiquinone, antiviral substance and somatomedin; The immunological competence of high aquatic animal can be obviously done in long-term interpolation, helps the viral growth that resists fish, can preventing disease; Increase resistance against diseases, reduce bait and throw in the raising bait utilization, improve surviving rate; Strengthen digestion, promote growth fast.
4, the short algae of rich water; The present invention can be an algae through bio-transformation with organic substances such as undissolved carbon, nitrogen phosphorus, silicon, potassium in the water; The battalion commander source that can absorb promotes algal grown, and can be used as the abundant open-mouthed bait of aquatic animal in seedling stage; It is obvious to culture effect of increasing production the fish, shrimps etc. that with the planktonic organism are food, and it is obvious that the present invention and fertilizer (the sharp element of giving birth to of rich water) are used the rich water effect.
5, safeguard the water quality eubiosis, the present invention forms dominant colony with contention nutrition of pathogeny mikrobe and living space after getting into water body, and harmful germ can't be bred in a large number; Can not form morbific envrionment conditions from face, aquatic animal is susceptible disease not just, and the present invention consumes the oxygen of water body in addition; Can be through being the oxygen consumption factor of little water body; Thereby produce the effect of indirect oxygenation, improve the transparency of water body, promote the photosynthesis of algae.
Embodiment
Below in conjunction with embodiment content of the present invention is described further.
Specific embodiment 1:
The described photosynthetic bacterium water quality cleansing agent of present embodiment is to be mixed with by photosynthetic bacterium liquid, calcium chloride and Sodium phosphate, dibasic to form; Said water quality cleansing agent is made up of supernatant and precipitated liquid.
The preparation method of the described photosynthetic bacterium water quality cleansing agent of present embodiment, it carries out successively according to the following steps:
1) preparation photosynthetic bacterium liquid is measured 1000kg photosynthetic bacterium liquid and is poured in the stirred vessel;
2) take by weighing calcium chloride 5kg, Sodium phosphate, dibasic 5kg; They are poured into respectively in the stirred vessel, stirred 5 minutes, leave standstill;
3) leave standstill one hour after, when the solution in the stirred vessel forms supernatant and sedimentation liquid when two-layer; With half extraction of supernatant, remainder is the photosynthetic bacterium water quality cleansing agent;
The preparation method of said photosynthetic bacterium liquid carries out successively according to the following steps:
1) in agitation vat, pours the 500kg tap water into;
2) in agitation vat, add following material respectively: SODIUM PHOSPHATE, MONOBASIC 3000g, sodium-acetate 1100g, Hydrocerol A 800g, ammonium chloride 550g, sodium hydroxide 1100g, iron(ic)chloride 200g, calcium chloride 50g, yeast extract paste 55g;
3) above-mentioned substance was stirred in agitation vat 5 minutes, make it dissolving;
4) in agitation vat, add 300～500kg photosynthetic bacterium bacterial classification stoste (buying from market), stirred 5 minutes, the liquid in the agitation vat pours in the cultivation pool then, the abundant illumination of assurance condition under cultivate, incubation time is 7～10 days; (will maintain the temperature at winter more than 20 ℃, maintain the temperature at summer below 35 ℃) cultivated completion when the color of solution becomes scarlet, make photosynthetic bacterium liquid.
In the present embodiment, the effect of said calcium chloride is to be used for allocating some water-fast calcium cpd depositions, and it can make the photosynthetic bacterium deposition that includes calcium cpd.Said Sodium phosphate, dibasic plays the effect of accessory substance, PH regulator.
The concrete mechanism of action and effect are following:
Can make the photosynthetic bacterium deposition after water-soluble calcium chloride adds in the photosynthetic bacterium liquid, thereby make this scavenging agent form supernatant and precipitated liquid.Through having added the Sodium phosphate, dibasic of accessory substance, PH modulator effect, prolonged the shelf time of photosynthetic bacterium in this scavenging agent again.Earlier this scavenging agent is shaken up during use, and then pour in the fishpond, a part of photosynthetic bacterium in this scavenging agent disperses in the pool water within Chi Shui to be purified, and most of photosynthetic bacterium is effectively decomposed the material that becomes sour at the bottom of being deposited to the pond.The cell count of this product unit weight is about 30,000,000,000 cell count/milliliters, has improved 4～7 times than like product; Its shelf time is about 2 years, prolongs 2～4 times than like product, has packing, advantage that transportation cost is low.
Table one is the performance comparison table of this scavenging agent and photosynthetic bacterium liquid concentrator and common photosynthetic bacterium liquid.
Table one
| Project | Photosynthetic bacterium content | Shelf time |
| This photosynthetic bacterium water quality cleansing agent after shaking up | 150～30,000,000,000 cell count/milliliters | 1～2 year |
| Photosynthetic bacterium liquid concentrator of the prior art | 10～5,000,000,000 cell count/milliliters | Half a year |
| Common photosynthetic bacterium liquid of the prior art | 2～500,000,000 cell count/milliliters | Half a year |
Specific embodiment 2:
The characteristics of present embodiment are: replace Sodium phosphate, dibasic with SODIUM PHOSPHATE, MONOBASIC, other are identical with specific embodiment 1.
Specific embodiment 3:
The characteristics of present embodiment are: in the preparation method of photosynthetic bacterium water quality cleansing agent, and described photosynthetic bacterium liquid, calcium chloride, the amount of taking by weighing of Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC is respectively: 1000kg, 1kg and 1kg.Other are identical with specific embodiment 1 or 2.
Specific embodiment 4:
The characteristics of present embodiment are: in the preparation method of photosynthetic bacterium water quality cleansing agent, and described photosynthetic bacterium liquid, calcium chloride, the amount of taking by weighing of Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC is respectively: 1000kg, 3kg and 3kg.Other are identical with specific embodiment 1 or 2.
Specific embodiment 5:
The characteristics of present embodiment are: in the preparation method of photosynthetic bacterium water quality cleansing agent, and described photosynthetic bacterium liquid, calcium chloride, the amount of taking by weighing of Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC is respectively: 1000kg, 7kg and 7kg.Other are identical with specific embodiment 1 or 2.
Specific embodiment 6:
The characteristics of present embodiment are: in the preparation method of photosynthetic bacterium water quality cleansing agent, and described photosynthetic bacterium liquid, calcium chloride, the amount of taking by weighing of Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC is respectively: 1000kg, 10kg and 10kg.Other are identical with specific embodiment 1 or 2.
Specific embodiment 7:
The characteristics of present embodiment are: in the preparation method of photosynthetic bacterium water quality cleansing agent, and described photosynthetic bacterium liquid, calcium chloride, the amount of taking by weighing of Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC is respectively: 1000kg, 15kg and 15kg.Other are identical with specific embodiment 1 or 2.
Specific embodiment 8:
The characteristics of present embodiment are: in the preparation method of photosynthetic bacterium water quality cleansing agent, and described photosynthetic bacterium liquid, calcium chloride, the amount of taking by weighing of Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC is respectively: 1000kg, 20kg and 20kg.Other are identical with specific embodiment 1 or 2.
Other embodiment:
The addition of said calcium chloride is confirmed by the quantity of contained photosynthetic bacterium in the scavenging agent; Its addition is not to be The more the better; When the calcium chloride that adds very little the time, photosynthetic bacterium is also precipitated few, when the calcium chloride that adds has made most of photosynthetic bacterium post precipitation in the scavenging agent when reaching peak value (be precipitation capacity) enough; Add calcium chloride again the precipitation capacity of photosynthetic bacterium is increased, only can increase production cost.The addition of said Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC many or I haven't seen you for ages directly influences the shelf time of scavenging agent; Its addition is not to be The more the better; When the calcium chloride that adds very little the time, the shelf time of scavenging agent is short more, when the Sodium phosphate, dibasic that adds or SODIUM PHOSPHATE, MONOBASIC foot have made the shelf time of scavenging agent reach peak (2 years); Add Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC again the shelf time of scavenging agent is prolonged, only can increase production cost.Therefore, change the addition of calcium chloride and Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC or adopt other materials to substitute Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC all belongs to protection scope of the present invention with same function.Photosynthetic bacterium liquid of the present invention also can adopt prepared by other of the prior art, also can from market, directly buy.
Claims (5)
1. photosynthetic bacterium water quality cleansing agent; It is characterized in that: said water quality cleansing agent mainly is mixed and made into by photosynthetic bacterium liquid, calcium chloride and Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC; Hundred parts of content of the weight of each material composition do; Photosynthetic bacterium liquid 96%～99.8%, calcium chloride 0.1%～2%, Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC 0.1%～2%.
2. a kind of photosynthetic bacterium water quality cleansing agent according to claim 1 is characterized in that: hundred parts of content of the weight of each material composition are: photosynthetic bacterium liquid 99%, calcium chloride 0.5%, Sodium phosphate, dibasic 0.5%.
3. a kind of photosynthetic bacterium water quality cleansing agent according to claim 1 is characterized in that: hundred parts of content of the weight of each material composition are: photosynthetic bacterium liquid 99%, calcium chloride 0.5%, SODIUM PHOSPHATE, MONOBASIC 0.5%.
4. preparation method of photosynthetic bacterium water quality cleansing agent according to claim 1 is characterized in that may further comprise the steps:
1) cultivates photosynthetic bacterium, and be prepared into photosynthetic bacterium liquid, measure 1000kg photosynthetic bacterium liquid and pour in the stirred vessel; The preparation method of described photosynthetic bacterium liquid carries out successively according to the following steps:
1. in agitation vat, pour the 500kg tap water into;
2. in agitation vat, add following material respectively: SODIUM PHOSPHATE, MONOBASIC 3000g, sodium-acetate 1100g, Hydrocerol A 800g, ammonium chloride 550g, sodium hydroxide 1100g, iron(ic)chloride 200g, calcium chloride 50g, yeast extract paste 55g;
3. above-mentioned substance was stirred in agitation vat 5 minutes, make it dissolving;
4. in agitation vat, add 300～500kg photosynthetic bacterium bacterial classification stoste, stirred 5 minutes, the liquid in the agitation vat pours in the cultivation pool then, under the condition that guarantees abundant illumination, cultivates, and incubation time is 7～10 days; To maintain the temperature at winter more than 20 ℃, maintain the temperature at summer below 35 ℃, when the color of solution becomes scarlet, cultivate completion, make photosynthetic bacterium liquid;
2) take by weighing calcium chloride 5kg, Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC 5kg; They are poured into respectively in the stirred vessel, stirred 5 minutes, leave standstill;
3) leave standstill one hour after, when the solution in the stirred vessel forms supernatant and sedimentation liquid when two-layer; Promptly make the photosynthetic bacterium water quality cleansing agent.
5. the preparation method of a kind of photosynthetic bacterium water quality cleansing agent according to claim 4; It is characterized in that: in step 3); When the solution in the stirred vessel forms supernatant and sedimentation liquid when two-layer, with half extraction of supernatant, remainder is spissated photosynthetic bacterium water quality cleansing agent again.
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| CN103087938A (en) * | 2011-11-04 | 2013-05-08 | 天津绿动植物营养技术开发有限公司 | Application of medium for rapid propagation of photosynthetic bacteria in slightly polluted water |
| CN105543093B (en) * | 2015-12-22 | 2019-10-01 | 天津凯英科技发展股份有限公司 | A kind of ammonia nitrogen degradation microbial inoculum preservative agent and store method |
| CN107098480B (en) * | 2017-03-23 | 2020-11-03 | 西安建筑科技大学 | Method for assisting algae dissolving bacteria to dissolve algae and in-situ mixing algae dissolving device |
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|---|---|---|---|---|
| CN1124293A (en) * | 1994-12-09 | 1996-06-12 | 王厚德 | Solid photosynthetic bacteria technology |
| CN1850660A (en) * | 2006-05-17 | 2006-10-25 | 天津生机集团有限公司 | Improved composition of base of aquatic cultivation pool |
| CN101407774A (en) * | 2008-11-20 | 2009-04-15 | 中国科学院广州地球化学研究所 | Preparation technique of photosynthetic bacteria preparation |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1124293A (en) * | 1994-12-09 | 1996-06-12 | 王厚德 | Solid photosynthetic bacteria technology |
| CN1850660A (en) * | 2006-05-17 | 2006-10-25 | 天津生机集团有限公司 | Improved composition of base of aquatic cultivation pool |
| CN101407774A (en) * | 2008-11-20 | 2009-04-15 | 中国科学院广州地球化学研究所 | Preparation technique of photosynthetic bacteria preparation |
Non-Patent Citations (4)
| Title |
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| JP特开2002-192187A 2002.07.10 |
| JP特开平10-146600A 1998.06.02 |
| 丁成.固定化光合细菌对富营养化水质的净化效果.《安徽农业科学》.2008,第36卷(第14期),第6028-6030页. |
| 固定化光合细菌对富营养化水质的净化效果;丁成;《安徽农业科学》;20081231;第36卷(第14期);第6028-6030页 * |
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