CN102816001A - Special amino acid fertilizer solution for aquaculture and preparation method thereof - Google Patents
Special amino acid fertilizer solution for aquaculture and preparation method thereof Download PDFInfo
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Abstract
The invention discloses special amino acid fertilizer solution for aquaculture and a preparation method thereof. The special amino acid fertilizer solution for aquaculture is prepared with amino acid solution, trace elements, inorganic fertilizers and probiotics according to a certain proportion. The preparation method comprises the steps of: A, production of the amino acid solution : a, hydrolysis; b, neutralization; c, filtration, and d, concentration; and B, production of amino acid fertilizer solution : a, chelation: heating the amino acid solution to 70-90 DEG C, adding the trace elements while mixing, and performing chelation for 2-3 hours; b, mixing: sequentially adding the inorganic fertilizers and the probiotics, and mixing; c, inspection: performing inspection according to relevant quality standards; d, packaging: metering and packaging according to packaging specifications required for different finished products; and e, storage and transportation: storing and transporting the finished products qualified in inspection and packaging. The immunity of fish can be improved, diseases can be reduced, the yield of spotted silver carp aquaculture can be increased, the cost of fish farming can be decreased, and the quality of the fish can be improved.
Description
Technical field
The invention belongs to the biological fish-fertilizer technical field, more specifically relate to the special-purpose amino acid rich water of a kind of aquaculture liquid, also relate to the method for a kind of amino acid rich water liquid of " waste water " preparation that utilizes in L-Gelucystine, L-leucine, the L-tyrosine production process simultaneously.This rich water liquid is a kind of biological fish-fertilizer of high-efficiency environment friendly, is applicable to each fish of freshwater aquiculture, shrimps, crab class, shellfish etc.
Technical background
Fertilizer commonly used in the culture fishery mainly contains three major types, i.e. fertilizer, inorganic fertilizer, biological fish-fertilizer.Traditional fertilizer has its advantage, but fertilizer efficiency is slow, and the process of being utilized by planktonic organism is longer; And in decomposing mineralisation process, consume a large amount of oxygen in the water body, this growth to fish is disadvantageous, and in most of the cases; Utilising efficiency is low, causes water pollution easily, in addition; Fertilizer should be made base manure, and is mainly used in the Winter-Spring water temperature than low season.Though inorganic fertilizer comes rapidly fertile, function is also comparatively single, uses a series of problems such as quality of bringing environment, water quality and fishery products in a large number.Biological fish-fertilizer has remedied the deficiency of fertilizer, inorganic fertilizer, and composite probiotics, trace element etc., is the main flow fertilizer of present aquaculture.
There is " waste water " of great deal of high concentration to produce with hair-hydrolyzation extraction L-Gelucystine, L-leucine, L-tyrosine, mainly contains 18 seed amino acids and ammonium chloride, if directly discharging has not only been wasted valuable resource, and polluted water surrounding.How to turn waste into wealth, turn harm into good, China scientific worker has passed through long-term exploration and research, and report is that " amino acid journal 1992.4 also has report successively to soil and fertilizer institute of the Chinese Academy of Sciences afterwards about " research of Fertilizer Efficiency of Amino Acids effect " the earliest.Research shows: amino acid can directly be absorbed by leaf, stem, root, can promote plant tillering, and fertilizer efficiency is fast, and kilnitamin is stronger than the effect of single amino acids, and the fertility ratio waits the inorganic nitrogenous fertilizer high about 50% of nitrogen amount.How " waste water " of L-Gelucystine, L-leucine, L-tyrosine being effectively used in the aquaculture and going, in order to improve the fertile effect of fishing, reduce the cost of breeding fish, is the problem that a lot of scientific workers study for a long period of time and explore, and is not resolved so far.
Amino acid " waste water " as fertilizer sources is used for aquaculture, and the applicant has carried out reaching research and the exploration in 12 years, and has obtained breakthrough.Through applicant's retrieval, also do not see special-purpose amino acid rich water liquid of a kind of aquaculture and preparation method at present and be disclosed or use.
Summary of the invention
The objective of the invention is to be to provide the special-purpose amino acid rich water of a kind of aquaculture liquid; Special growth characteristics to fish and food organism thereof; Adopt modern biotechnology to produce amino acid; Carry out chelating with trace element again, composite then mineral manure, probiotics organically combine inorganic quick-acting, organic long-acting, bio-synergistic; The meticulous special-purpose amino acid rich water of the aquaculture of development liquid, 18 kinds of total free aminoacidss that are rich in that planktonic organism very easily digests and assimilates and necessary 10 seed amino acids of fish growth.After making fishing fertilizer apply water body, can satisfy the nutritional needs of fish growth and breed fish, can improve water quality again, improve fish immunity power, reduce disease and take place, increase the variegated carp cultured output, reduce the cost of breeding fish, improve the fish quality with the water body of fostering and apply fertilizer.
Another object of the present invention is the preparation method who has been to provide the special-purpose amino acid rich water of a kind of aquaculture liquid; Easy to implement the method; Easy and simple to handle; With " waste water " in the L-Gelucystine production process through concentrate, chelating and preparation amino acid rich water liquid organically combines, and both solved amino acid whose " waste water " problem, and the amino acid resource of a large amount of cheapness is provided for culture fishery again.If remove painstakingly to add amino acid in the fertile preparation of fishing, that is a kind of luxury goods.
The technical scheme of amino acid rich water liquid provided by the present invention is:
The present invention is used for aquaculture with amino acid " waste water " as fertilizer sources, and the efficient utilization of resource and recycle and environment protection are closely combined, and in order to improve the fertile effect of fishing, reduces the cost of breeding fish.18 kinds of total free aminoacidss that amino acid rich water liquid of the present invention is rich in that planktonic organism very easily digests and assimilates and necessary 10 seed amino acids of fish growth; Methionin, L-glutamic acid, leucine glycocoll particularly wherein; Plant plankton there is peculiar photosynthesis; Can increase the water body dissolved oxygen, improve water body environment, effectively avoid of the harm of dissolved oxygen deficiency fish.After fishing fertilizer applies water body, can satisfy the nutritional needs of fish growth and breed fish, can improve water quality again, improve fish immunity power, reduce the fish diseases generation, increase the output of breeding fish, reduce the cost of breeding fish, improve the fish quality with the water body of fostering and apply fertilizer.
1. the special-purpose amino acid rich water of aquaculture liquid, it is processed by the raw material of following massfraction:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 64～74 trace elements 0.5～1
Mineral manure 24～35 probioticss 0.5～1
Described aminoacids solution is behaved and is sent out, pig hair, chicken feather, drake feather, goose feather, wool, ox hair wherein any one through hydrochloric acid hydrolysis; Obtain a kind of aminoacids solution, in aminoacids solution, containing each amino acid masses per-cent through detection is aspartic acid 1.3～2.5, Threonine 1.0～1.6, Serine 1.8～4.0, L-glutamic acid 2.0～3.5, proline(Pro) 2.0～3.0, glycocoll 2.0～3.5, L-Ala 1.6～2.0, Gelucystine 0.1～0.2, Xie Ansuan 1.5～2.0, methionine(Met) 0.1～0.2, Isoleucine 0.5～1.5, leucine 0.6～0.8, tyrosine 0.2～0.3, phenylalanine(Phe) 1.2～1.5, Methionin 0.3～0.4, Histidine 0.1～0.2, tryptophane 0.1～0.2, l-arginine 1.5～2.0.
Described trace element comprises ferrous sulfate, sal epsom, manganous sulfate, ammonium molybdate, zinc sulfate, rose vitriol, Sodium Tetraborate, water glass, sodium selenate a kind of or two to eight kinds of arbitrary combination wherein;
Described mineral manure comprises urea, ammonium chloride, ammonium sulfate, bicarbonate of ammonia, an ammonium nitrate, ammonium humate, monoammonium phosphate, diammonium phosphate, fused(calcium magnesium)phosphate, superphosphate of lime, double superhosphate a kind of or two to 11 kinds of arbitrary combination wherein;
Described probiotics comprises milk-acid bacteria, bifidus bacillus, genus bacillus, yeast, nitrobacteria, photosynthetic bacterium a kind of or two to six kinds of arbitrary combination wherein.
The preparation method of the special-purpose amino acid rich water of a kind of aquaculture liquid the steps include:
A. the preparation of aminoacids solution:
A. hydrolysis
Hair (people sends out, pig hair, chicken feather, drake feather, goose feather, wool, ox hair wherein any one): hydrochloric acid (28～31%)=1:2, temperature is controlled at 108～115 ℃, and the reaction times is at 6～8h;
B. neutralization
Be neutralized to pH4.8～5.1 with under 40～70 ℃ in 99.9% liquefied ammonia;
C. filter (comprising rough and refining)
Filter cake extracts L-Gelucystine, L-leucine, L-tyrosine, and it is subsequent use that filtrating changes enrichment process over to;
D. concentrate
To filtrate-0.06～-be concentrated into 28～30Be ＇ under the 0.08Mpa, aminoacids solution.
B. the preparation of amino acid rich water liquid:
A. chelating
Aminoacids solution is heated to 70～90 ℃, stirs trace element chelated 2～3 hours of adding down;
B. be mixed
Add mineral manure, probiotics successively, stirring and evenly mixing;
C. check
Test according to the correlated quality standard;
D. pack
Different finished products packing specifications is on request carried out metering packing;
E. accumulating
To be up to the standards, the storage of the finished product of Proper Packing, transportation.
The present invention compares with the various fishing fertilizer of other uses, has following outstanding advantage:
1. 18 kinds of total free aminoacidss that are rich in that planktonic organism very easily digests and assimilates and necessary 10 seed amino acids of fish growth;
2. Methionin, L-glutamic acid, leucine glycocoll particularly has peculiar photosynthesis to plant plankton, can increase the water body dissolved oxygen amount, reduces fish and raises the nose above water to breathe the general pool;
3. various nutritive element proportioning science, fish were both direct-edible, and the water body food organism of can fostering and apply fertilizer again are edible indirectly by fish, and feed, fertilizer coefficient are low, can save concentrated feed 33～45%;
4. post-directed training fish high-quality food organism breed zooplankton in a large number, have strengthened the input ratio of flower silver carp, are increased to 7:3 by traditional 1:3;
5. probiotics can effectively be improved water quality, decomposes the residual bait of fish, ight soil, organic matter, reduces the content of ammonia nitrogen, nitrite; Effectively suppressed the breeding growth of harmful bacterium; Improve fish body immunizing power, effectively prevented the sickness rate of fish hemorrhage, enteritis, rotted gill disease, reduced death;
6. consumption is few, and instant effect is easy to use, and fragrant odour has the distinctive sauce fragrance of dense amino acid, polluted-water not, and at the bottom of the pond of not hardening, environmental protection, efficient cheap;
7. input-output ratio is high, and per kilogram amino acid rich water liquid can be produced 2.2 kilograms of fresh fish;
8. turn the foul and rotten into the rare and ethereal, efficient utilization and the recycle of amino acid " waste water " with resource organically combined, realized excellent transfer by " luxury goods " to " popular ".
Description of drawings
Fig. 1 is a kind of aminoacids solution process flow sheet
Fig. 2 is a kind of amino acid rich water liquid process flow sheet
Embodiment
The special-purpose amino acid rich water of a kind of aquaculture liquid, its proportioning is following:
Table 1 amino acid fertilizer aqueous dispensing fluid instance unit: mass percent
Described aminoacids solution, in aminoacids solution, containing each amino acid masses per-cent through detection is aspartic acid 1.3～2.5, Threonine 1.0～1.6, Serine 1.8～4.0, L-glutamic acid 2.0～3.5, proline(Pro) 2.0～3.0, glycocoll 2.0～3.5, L-Ala 1.6～2.0, Gelucystine 0.1～0.2, Xie Ansuan 1.5～2.0, methionine(Met) 0.1～0.2, Isoleucine 0.5～1.5, leucine 0.6～0.8, tyrosine 0.2～0.3, phenylalanine(Phe) 1.2～1.5, Methionin 0.3～0.4, Histidine 0.1～0.2, tryptophane 0.1～0.2, l-arginine 1.5～2.0.
Described trace element comprises ferrous sulfate 15%, sal epsom 15%, manganous sulfate 5%, ammonium molybdate 5%, zinc sulfate 15%, rose vitriol 5%, Sodium Tetraborate 15%, water glass 20%, sodium selenate 5% a kind of or two to eight kinds of arbitrary combination wherein;
Described mineral manure comprises urea 10%, ammonium chloride 20%, ammonium sulfate 10%, bicarbonate of ammonia 10%, an ammonium nitrate 5%, ammonium humate 5%, monoammonium phosphate 20%, diammonium phosphate 5%, fused(calcium magnesium)phosphate 5%, superphosphate of lime 5%, double superhosphate 5% a kind of or two to 11 kinds of arbitrary combination wherein;
Described probiotics comprises milk-acid bacteria 10%, bifidus bacillus 10%, genus bacillus 30%, yeast 15%, nitrobacteria 15%, photosynthetic bacterium 20% a kind of or two to six kinds of arbitrary combination wherein.
The preparation method of the special-purpose amino acid rich water of a kind of aquaculture liquid the steps include:
A. the production of aminoacids solution (seeing accompanying drawing 1 for details)
A. hydrolysis 1.
Hair (people sends out, pig hair, chicken feather, drake feather, goose feather, wool, ox hair wherein any one): hydrochloric acid (28～31%)=1:2, temperature are controlled at 108 or 110 or 111 or 113 or 114 or 115 ℃, the reaction times 6 or 7 or 8h;
B. neutralization 2.
Be neutralized to pH4.8 or 4.9 or 5 or 5.1 with under 99.9% liquefied ammonia 40 or 43 or 46 or 48 or 51 or 55 or 58 or 61 or 64 or 66 or 68 or 70 ℃;
C. filter (comprise rough 3. with refining 4.)
Filter cake extracts L-Gelucystine, L-leucine, L-tyrosine, and it is subsequent use that filtrating changes enrichment process over to;
D. concentrate 5.
To filtrate-0.06 or-0.07 or-be concentrated into 28 or 29 or 30Be ＇ under the 0.08Mpa, aminoacids solution 6..
B. the production of amino acid rich water liquid (seeing accompanying drawing 2 for details)
A. chelating 7.
Aminoacids solution stirs trace element chelated 2 or 2.5 or 3 hours of adding down at 70 or 75 or 80 or 85 or 90 ℃;
B. be mixed 8.
Add mineral manure, probiotics successively, stirring and evenly mixing;
C. check 9.
Test according to the correlated quality standard;
D. packing 10.
Different finished products packing specifications is on request carried out metering packing;
E. accumulating
To be up to the standards, the storage of the finished product of Proper Packing, transportation.
1. to the requirement of aquaculture water
The quality of aquaculture water is great to the growth relationship of fish, and so-called " breed fish is exactly to support water " is exactly this reason.Therefore, require during the whole breed of breeding fish, to require dissolved oxygen>=5mgL, ammonia nitrogen≤0.02mg/L, nitrite≤0.1mg/L.
2. culture and use and effect
Evenly be spilled in the aquaculture water behind the special-purpose amino acid rich water of the aquaculture of the present invention liquid dilute with water, effect is following:
Culture application example:
80 mu of fishponds, Jiangxia, Wuhan in 2012, average depth 1.6m puts silver carp 21000 tails in a suitable place to breed; Variegated carp 7000 tails, use our factory's amino acid rich water liquid before, water transparency 44cm; Use our factory's amino acid rich water liquid 16 days～June 16 May in 2012; Transparency 30cm, 0.56 jin of silver carp moon weightening finish, 0.78 jin of variegated carp moon weightening finish.
Claims (10)
1. the special-purpose amino acid rich water of aquaculture liquid, it is processed by the raw material of following massfraction:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 64～74 trace elements 0.5～1
Mineral manure 24～35 probioticss 0.5～1;
Described aminoacids solution is behaved and is sent out, pig hair, chicken feather, drake feather, goose feather, wool, ox hair wherein any one through hydrochloric acid hydrolysis; Obtain a kind of aminoacids solution, in aminoacids solution, containing each amino acid masses per-cent through detection is aspartic acid 1.3～2.5, Threonine 1.0～1.6, Serine 1.8～4.0, L-glutamic acid 2.0～3.5, proline(Pro) 2.0～3.0, glycocoll 2.0～3.5, L-Ala 1.6～2.0, Gelucystine 0.1～0.2, Xie Ansuan 1.5～2.0, methionine(Met) 0.1～0.2, Isoleucine 0.5～1.5, leucine 0.6～0.8, tyrosine 0.2～0.3, phenylalanine(Phe) 1.2～1.5, Methionin 0.3～0.4, Histidine 0.1～0.2, tryptophane 0.1～0.2, l-arginine 1.5～2.0;
Described trace element comprises ferrous sulfate, sal epsom, manganous sulfate, ammonium molybdate, zinc sulfate, rose vitriol, Sodium Tetraborate, water glass, sodium selenate a kind of or two to eight kinds of arbitrary combination wherein;
Described mineral manure comprises urea, ammonium chloride, ammonium sulfate, bicarbonate of ammonia, an ammonium nitrate, ammonium humate, monoammonium phosphate, diammonium phosphate, fused(calcium magnesium)phosphate, superphosphate of lime, heavy persulfuric acid calcium a kind of or two to 11 kinds of arbitrary combination wherein;
Described probiotics comprises milk-acid bacteria, bifidus bacillus, genus bacillus, yeast, nitrobacteria, photosynthetic bacterium a kind of or two to six kinds of arbitrary combination wherein.
2. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 74 trace elements 0.5
Mineral manure 25 probioticss 0.5.
3. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 72.8 trace elements 0.6
Mineral manure 26 probioticss 0.6.
4. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 71.6 trace elements 0.7
Mineral manure 27 probioticss 0.7.
5. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 70.4 trace elements 0.8
Mineral manure 28 probioticss 0.8.
6. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 69.4 trace elements 0.8
Mineral manure 29 probioticss 0.8.
7. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 68.6 trace elements 0.7
Mineral manure 30 probioticss 0.7.
8. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 67.8 trace elements 0.6
Mineral manure 31 probioticss 0.6.
9. the special-purpose amino acid rich water of a kind of aquaculture according to claim 1 liquid is characterized in that:
Raw materials quality per-cent raw materials quality per-cent
Aminoacids solution 67 trace elements 0.5
Mineral manure 32 probioticss 0.5.
10. the preparation method of the special-purpose amino acid rich water of the described a kind of aquaculture of claim 1 liquid the steps include:
A. the production of aminoacids solution
A. hydrolysis
Hair: hydrochloric acid=1:2,108～115 ℃ of reaction 6～8h;
B. neutralization
Be neutralized to PH4.8～5.1 with under 40～70 ℃ in 99.9% liquefied ammonia;
C. filter
Filter cake extracts L-Gelucystine, L-leucine, L-tyrosine, and it is subsequent use that filtrating changes enrichment process over to;
D. concentrate
To filtrate-0.06～-be concentrated into 28～30Be ＇ under the 0.08MPa, aminoacids solution;
B. the production of amino acid rich water liquid
A. chelating
Aminoacids solution is heated to 70～90 ℃, stirs trace element chelated 2～3 hours of adding down;
B. be mixed
Add mineral manure, probiotics successively, stirring and evenly mixing;
C. check
Test according to the correlated quality standard;
D. pack
Different finished products packing specifications is on request carried out metering packing;
E. accumulating
To be up to the standards, the storage of the finished product of Proper Packing, transportation.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104817356A (en) * | 2015-04-22 | 2015-08-05 | 天津贾立明蚯蚓养殖有限公司 | Aquatic product aquaculture water rich agent and preparation process thereof |
| CN105154361A (en) * | 2015-08-31 | 2015-12-16 | 湖北中扬生态农业有限公司 | Micro-ecological preparation for medium-sized and small-sized reservoir ecological aquaculture feed plankton cultivation |
| CN107432263A (en) * | 2017-07-21 | 2017-12-05 | 平南县德湖种养农民专业合作社 | The foster method of full ecological fish duck set |
| CN108558544A (en) * | 2018-06-22 | 2018-09-21 | 砀山县绿源生态肥料有限公司 | A kind of aquatic amino acid phosphorus calcareous fertilisers |
| CN108863606A (en) * | 2018-09-03 | 2018-11-23 | 安徽省文胜生物工程股份有限公司 | A kind of preparation method of high-concentration biological bacterium amino acid water-soluble fertilizer |
| CN114751793A (en) * | 2022-04-25 | 2022-07-15 | 武汉都兴隆农业科技有限公司 | New effect of amino acid nitrogen fertilizer in inhibiting growth of moss in shrimp farming field and preparation method thereof |
| CN115745195A (en) * | 2022-12-12 | 2023-03-07 | 御兴堂(北京)生物科技有限责任公司 | Water quality treating agent for aquaculture and application thereof |
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| CN101225007A (en) * | 2008-02-03 | 2008-07-23 | 武汉市科洋生物工程有限公司 | Special biological fish-fertilizer for aquaculture and preparation method thereof |
| CN101836692A (en) * | 2010-05-18 | 2010-09-22 | 武汉市洪山吕墩氨基酸厂 | Amino acid biological fish fertilizer and preparation method thereof |
| CN102047857A (en) * | 2010-11-16 | 2011-05-11 | 岳阳神力生物科技有限公司 | Novel high-efficiency biological fish manure |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104817356A (en) * | 2015-04-22 | 2015-08-05 | 天津贾立明蚯蚓养殖有限公司 | Aquatic product aquaculture water rich agent and preparation process thereof |
| CN105154361A (en) * | 2015-08-31 | 2015-12-16 | 湖北中扬生态农业有限公司 | Micro-ecological preparation for medium-sized and small-sized reservoir ecological aquaculture feed plankton cultivation |
| CN105154361B (en) * | 2015-08-31 | 2018-09-18 | 湖北中扬生态农业有限公司 | The probiotics cultivated for medium and small reservoirs ecologic breeding bait planktonic organism |
| CN107432263A (en) * | 2017-07-21 | 2017-12-05 | 平南县德湖种养农民专业合作社 | The foster method of full ecological fish duck set |
| CN108558544A (en) * | 2018-06-22 | 2018-09-21 | 砀山县绿源生态肥料有限公司 | A kind of aquatic amino acid phosphorus calcareous fertilisers |
| CN108863606A (en) * | 2018-09-03 | 2018-11-23 | 安徽省文胜生物工程股份有限公司 | A kind of preparation method of high-concentration biological bacterium amino acid water-soluble fertilizer |
| CN114751793A (en) * | 2022-04-25 | 2022-07-15 | 武汉都兴隆农业科技有限公司 | New effect of amino acid nitrogen fertilizer in inhibiting growth of moss in shrimp farming field and preparation method thereof |
| CN115745195A (en) * | 2022-12-12 | 2023-03-07 | 御兴堂(北京)生物科技有限责任公司 | Water quality treating agent for aquaculture and application thereof |
| CN115745195B (en) * | 2022-12-12 | 2023-08-18 | 御兴堂(北京)生物科技有限责任公司 | Water quality treating agent for aquaculture and application thereof |
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