CN105154361A - Micro-ecological preparation for medium-sized and small-sized reservoir ecological aquaculture feed plankton cultivation - Google Patents
Micro-ecological preparation for medium-sized and small-sized reservoir ecological aquaculture feed plankton cultivation Download PDFInfo
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- CN105154361A CN105154361A CN201510549364.XA CN201510549364A CN105154361A CN 105154361 A CN105154361 A CN 105154361A CN 201510549364 A CN201510549364 A CN 201510549364A CN 105154361 A CN105154361 A CN 105154361A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention belongs to the field of aquaculture and discloses a micro-ecological preparation for medium-sized and small-sized reservoir ecological aquaculture feed plankton cultivation. The micro-ecological preparation for medium-sized and small-sized reservoir ecological aquaculture feed plankton cultivation comprises an active yeast preparation; mixture of the active yeast preparation and a nitrobacteria preparation according to weight part ratio of 1:1; mixture of bifidobacterium preparation and a bacillus subtilis preparation according to weight part ratio of 1:1; mixture of the bifidobacterium preparation and the nitrobacteria preparation according to weight part ratio of 1:1; or mixture of the active yeast preparation, the bifidobacterium preparation and the nitrobacteria preparation according to weight part ratio of 1:1:1; mixture of the bifidobacterium preparation, the bacillus subtilis preparation and the nitrobacteria preparation according to weight part ratio of 1:1:1. After the micro-ecological preparation disclosed by the invention is prepared into re-fermented fertilizers which are put into a water body, not only can the water of a reservoir be prevented from being polluted, but also the aquaculture yield of silver carp and bighead carp can be improved by means of feed increase, and fish products can also be guaranteed to have higher quality.
Description
Technical field
The invention belongs to aquaculture field, relate to cultivation probiotics.
Background technology:
In China, breed fish to raise together with aquaculture model for master in medium and small reservoirs, operating method has " intensive culture " and " extensive cultivation " two kinds, and point input bait and natural method of putting in a suitable place to breed are carried out.Medium and small reservoirs raises together with cultivation generally based on filter-feeding silver carp, bighead.In intensive culture pattern, generally carry out fertilising rich water according to different conditions such as climate change and water nutrition situations, fertilization time and quantity are determined in change (medium-sized reservoir is at 60 ~ 80 centimetres) with reference to water transparency, ensure that reservoir water colour is in green or light green.Fertilising is general adopts fertilizer or inorganic fertilizer, and fertilizer drops into aquaculture water after usually fermenting in the small-sized fermentation pond of fish pond and reservoir side, but often owing to devoting exclusive attention to output, causes excessive use of fertilizer and cause the pollution of reservoir water body.And extensive cultivation pattern is preyed on by a fairly large number of silver carp bighead due to hydroplankton, population can not get supplementing in time, and often cause fish food deficient, the speed of growth is slow, and output is not high.
Generally, breeding environment is improved in reservoir cultivation and the way of increasing economic efficiency is adjustment cultivation structure.The stocking rate of the ominivore-fish such as bream, carp and crucian is strengthened gradually to improve the transformation efficiency of food on the one hand in reservoir.Increase the input of famous-brand and high-quality fish on the other hand, as mandarin fish, Yellow catfish and Culter class etc.But traditional method, cannot meet that silver carp bighead output increases, quality guarantee and keep the multiple target of water quality.
Research and development medium and small reservoirs zooplankton Cultivating techniques is the necessary ways under fresh water fishery breeding ecological development trend, the exploitation of technology and promote for improving medium and small reservoirs fishery cultivating, ecological environmental protection, fishery increased quality and ensureing fishery foods that safely etc. aspect is all very necessary.
Summary of the invention:
The object of the invention is to develop a kind of Tiny ecosystem reagent cultivated for medium and small reservoirs ecological fishery cultivating bait, overcome traditional reservoir fisheries aquaculture model polluted water, improve original probiotics and improve the deficiency that water quality and water prevention product disease new technology can not improve output very well.By using novel Tiny ecosystem reagent, under micro-oxygen conditions, fermentative processing is again carried out to the fertilizer of initial fermentation, then use the fertilizer after fermentation again to cultivate planktonic algae and zooplankton, utilize the planktonic algae of cultivation and zooplankton in medium and small reservoirs, carry out silver carp bighead ecologic breeding.The technology of the present invention both can ensure that Reservoir Water Quality was not contaminated, can be improved again the cultured output of silver carp bighead, ensure that fish product has higher quality by the approach increasing bait.
Technical solution of the present invention is as follows:
For the probiotics that medium and small reservoirs ecologic breeding bait planktonic organism is cultivated, comprise
Live yeast bacteria preparation; Or
Live yeast bacteria preparation and nitrobacteria preparation 1:1 mixture by weight; Bifidobacterium preparations and bacillus subtilis formulation be 1:1 mixture by weight; Bifidobacterium preparations and nitrobacteria preparation 1:1 mixture by weight; Or
Live yeast bacteria preparation, bifidobacterium preparations, nitrobacteria preparation 1:1:1 mixture by weight; Bifidobacterium preparations, bacillus subtilis formulation, nitrobacteria preparation 1:1:1 mixture by weight;
During use, by probiotics to the fertilizer after just fermentation according to weight ratio 1:25 ratio, micro-oxygen conditions 25 DEG C fermentation 72 hours the fertilizer that ferment again;
The fertilizer that will ferment again is rendered to and is cultivated in water tank, and at room temperature carry out planktonic organism chlorella and the straight Magna cultivation of hook foot, injected volume is that every 5 grams of fertilizers that ferment again are rendered in 12 premium on currency.
The invention still further relates to and utilize aforementioned probiotics to cultivate the method for plant plankton, by above-mentioned probiotics to the fertilizer after just fermentation according to weight ratio 1:25 ratio, micro-oxygen conditions 25 DEG C fermentation 72 hours the fertilizer that ferment again; The fertilizer that will ferment again is rendered to and is cultivated in water tank, and at room temperature carry out planktonic organism chlorella and the straight Magna cultivation of hook foot, injected volume is that every 5 grams of fertilizers that ferment again are rendered in 12 premium on currency.
In order to better technique effect, can microecosystem regulation preparation pH6.5-7.0;
When making commodity, bacteria preparation can be sold or selling after fertilizer packaging of fermenting again.
For the fertilizer that medium and small reservoirs ecologic breeding bait planktonic organism is cultivated, its preparation method is as follows:
(1) probiotics is prepared:
Get live yeast bacteria preparation; Or
Live yeast bacteria preparation and nitrobacteria preparation 1:1 mixture by weight; Bifidobacterium preparations and bacillus subtilis formulation be 1:1 mixture by weight; Bifidobacterium preparations and nitrobacteria preparation 1:1 mixture by weight; Or
Live yeast bacteria preparation, bifidobacterium preparations, nitrobacteria preparation 1:1:1 mixture by weight; Bifidobacterium preparations, bacillus subtilis formulation, nitrobacteria preparation 1:1:1 mixture by weight;
(2) by probiotics to the fertilizer after just fermentation according to weight ratio 1:25 ratio, micro-oxygen conditions 25 DEG C fermentation 72 hours the fertilizer that ferment again.
To ferment again the using method of fertilizer: the fertilizer that will ferment again is rendered in 12 premium on currency according to the every 5 grams fertilizers that ferment again.How many according to reservoir water body, can often within 30-40 days, throw in once.
The preparation of described live yeast bacteria preparation: get glucose 6 ~ 10g, peptone 3 ~ 5g, yeast extract paste 1.5 ~ 2.5g, distilled water 850 ~ 900ml, mix, autoclaving 20 ~ 25 minutes at 120 ~ 125 DEG C, adds 2 ~ 5g active yeast powder, the lower 25 DEG C of fermentations of anaerobic condition 72 ~ 96 hours, obtain live yeast bacteria preparation;
The preparation of described bifidobacterium preparations: get glucose 10 ~ 12g, peptone 7 ~ 9g, yeast extract paste powder 1 ~ 2g, Zulkovsky starch 0.2 ~ 0.3g, sodium-chlor 2 ~ 3g, halfcystine 0.2 ~ 0.3g, pork liver powder 0.2 ~ 0.3g, tween-80 0.4 ~ 0.6g, agar 7 ~ 9g, distilled water 600ml, tomato leach liquor 400ml, mixes, high pressure (100Pa) sterilizing 15 ~ 20 minutes at 120 ~ 125 DEG C, add 2 ~ 5g bifidobacteria viable bacteria kind, the lower 25 DEG C of fermentations of anaerobic condition 36 ~ 48 hours, obtain bifidobacterium preparations; Described tomato leach liquor preparation method: fresh tomato weighs cleans chopping, adds equivalent distilled water and heats in 100 DEG C of water-baths, stir 1.5 ~ 2 hours, by filtered through gauze, corrects pH=7;
The preparation of described bacillus subtilis formulation: get glucose 10 ~ 12g, peptone 7 ~ 9g, sodium-chlor 2 ~ 3g, extractum carnis 0.2 ~ 0.3g, agar 10 ~ 12g, distilled water 1000ml, mix, autoclaving 25 ~ 30 minutes at 120 ~ 125 DEG C, add the bacillus subtilis formulation of 2 ~ 5g activation, the lower 25 DEG C of fermentations of anaerobic condition 36 ~ 48 hours, obtain bacillus subtilis formulation;
The preparation of described nitrobacteria preparation: get ammonium sulfate 0.4 ~ 0.6g, sodium-chlor 0.2 ~ 0.4g, ferrous sulfate 0.02 ~ 0.04g, SODIUM PHOSPHATE, MONOBASIC 2 ~ 3g, magnesium sulfate 0.02 ~ 0.04g, calcium chloride 6 ~ 8g, agar 18 ~ 20g, distilled water 1000ml, mixes, and regulates pH=7.4 ~ 7.6, autoclaving 20 ~ 25 minutes at 120 ~ 125 DEG C, add the active nitrobacteria pulvis of 2 ~ 5g, ferment under aerobic conditions 48 ~ 72 hours, obtains nitrobacteria preparation.
The strain characteristic function that the present invention selects is as follows:
1, yeast: yeast can grow in the environment of aerobic and anaerobic, is facultative anaerobe, when aerobic, breaks sugar into carbonic acid gas and water and Yeast Growth is very fast.When anoxic, yeast breaks sugar into alcohol and carbonic acid gas.
2, bifidus bacillus: the Gram-positive bacillus being a kind of anaerobism, can maintain colony balance, suppresses the growth of pathogenic bacteria, has the function of non-specific and specific immune response improving digestibility, enhancing body.
3, subtilis: have amylase and protease activity, be widely used in feed, can promote the growth of animal, improves the utilization ratio of feed.In addition, this bacterial classification is applied also quite extensive in sewage disposal and bio-fertilizer fermentation or fermentation bed making, is a kind of multi-functional microorganism, can be used in combination with multiple bacterial classification, has vital role aborning.
4, nitrobacteria: be a kind of aerobic bacteria, comprises Nitrosomas and Nitromonas.Important role is play in nitrogen cycle purification of water quality process.
Beneficial effect of the present invention is as follows:
The present invention utilizes yeast, bifidus bacillus, the anaerobism characteristic of subtilis and the good oxygen characteristic of nitrobacteria, the fertilizer that just ferments is fermented again and increases the availability of fertilizer, the fertilizer fermented again is utilized to carry out the cultivation of planktonic organism (chlorella and cladocera) bait, the planktonic organism cultivated is used to carry out the cultivation of reservoir silver carp bighead, not only can increase the aquatic products amount of silver carp bighead, and can prevent from directly applying fertilizer the water pollution caused in reservoir, under the water ecological environment maintaining reservoir health, carry out fish farming simultaneously, the higher quality of fishery product can be ensured.Directly throw in reservoir with traditional reservoir fisheries aquaculture model and probiotics to carry out disease control and compare with the new technology improving water quality, there is multiple advantage.
Accompanying drawing explanation
Fig. 1 control group chlorella and the straight Magna of hook foot cultivate density and change in time.A: yeast, b: bifidus bacillus, c: subtilis, d: nitrobacteria
Fig. 2 control group dissolved oxygen and pH value change in time.A: yeast, b: bifidus bacillus, c: subtilis, d: nitrobacteria
Fig. 3 bigeminy bacterium test group chlorella and the straight Magna of hook foot cultivate density and change in time.A: yeast and bifidus bacillus (1:1), b: yeast and subtilis (1:1), c: yeast and nitrobacteria (1:1), d: bifidus bacillus and subtilis (1:1), e: bifidus bacillus and nitrobacteria (1:1), f: subtilis and nitrobacteria (1:1)
Fig. 4 bigeminy bacterium test group dissolved oxygen and pH value change in time.A: yeast and bifidus bacillus (1:1), b: yeast and subtilis (1:1), c: yeast and nitrobacteria (1:1), d: bifidus bacillus and subtilis (1:1), e: bifidus bacillus and nitrobacteria (1:1), f: subtilis and nitrobacteria (1:1)
Fig. 5 tri-bacterium test group chlorella and the straight Magna of hook foot cultivate density and change in time.A: yeast, bifidus bacillus, bacillus subtilis formulation (1:1:1), b: yeast, bifidus bacillus, nitrobacteria preparation (1:1:1), c: yeast, subtilis, nitrobacteria preparation (1:1:1), d: bifidus bacillus, subtilis, nitrobacteria preparation (1:1:1);
Fig. 6 side three bacterium test group dissolved oxygen and pH value change in time.A: yeast, bifidus bacillus, subtilis (1:1:1), b: yeast, bifidus bacillus, nitrobacteria (1:1:1), c: yeast, subtilis, nitrobacteria (1:1:1), d: bifidus bacillus, subtilis, nitrobacteria (1:1:1).
Embodiment:
Below in conjunction with accompanying drawing, case study on implementation of the present invention is described further:
Embodiment one
One, the preparation of reservoir ecologic breeding probiotics:
1, the preparation of live yeast bacteria preparation
Get glucose 6 ~ 10g, peptone 3 ~ 5g, yeast extract paste 1.5 ~ 2.5g, distilled water 850 ~ 900ml, mixes, high pressure (100Pa) sterilizing 20 ~ 25 minutes at 120 ~ 125 DEG C, add 2 ~ 5g active yeast powder (Angel Yeast Co., Ltd, Yichang City, Hubei Province, product standard code name: GB/T20886), the lower 25 DEG C of fermentations of anaerobic condition 72 ~ 96 hours, obtain live yeast bacteria preparation.
2, the preparation of bifidobacterium preparations
Get glucose 10 ~ 12g, peptone 7 ~ 9g, yeast extract paste powder 1 ~ 2g, Zulkovsky starch 0.2 ~ 0.3g, sodium-chlor 2 ~ 3g, halfcystine 0.2 ~ 0.3g, pork liver powder 0.2 ~ 0.3g, tween (80) 0.4 ~ 0.6g, agar 7 ~ 9g, distilled water 600ml, tomato leach liquor 400ml (tomato leach liquor preparation method: fresh tomato weighs cleans chopping, add equivalent distilled water to heat in 100 DEG C of water-baths, stir 1.5 ~ 2 hours, by filtered through gauze, correct pH=7), mix, high pressure (100Pa) sterilizing 15 ~ 20 minutes at 120 ~ 125 DEG C, add 2 ~ 5g bifidobacteria viable bacteria kind (Hebei Yiran Biological Technology Co., Ltd., From Shijiazhuang City of Hebei Province Zhengding County, products production credit number: QS130128010007), the lower 25 DEG C of fermentations of anaerobic condition 36 ~ 48 hours, obtain bifidobacterium preparations for subsequent use.
3, the preparation of bacillus subtilis formulation
Get glucose 10 ~ 12g, peptone 7 ~ 9g, sodium-chlor 2 ~ 3g, extractum carnis 0.2 ~ 0.3g, agar 10 ~ 12g, distilled water 1000ml, mix, high pressure (100Pa) sterilizing 25 ~ 30 minutes at 120 ~ 125 DEG C, add bacillus subtilis formulation (Wuhan Nature, Wuhan City, Hubei Province, the product standard card number: Q/WTH05-2012) of 2 ~ 5g activation, the lower 25 DEG C of fermentations of anaerobic condition 36 ~ 48 hours, obtain bacillus subtilis formulation for subsequent use.
4, the preparation of active nitrobacteria preparation
Get ammonium sulfate 0.4 ~ 0.6g, sodium-chlor 0.2 ~ 0.4g, ferrous sulfate 0.02 ~ 0.04g, SODIUM PHOSPHATE, MONOBASIC 2 ~ 3g, magnesium sulfate 0.02 ~ 0.04g, calcium chloride 6 ~ 8g, agar 18 ~ 20g, distilled water 1000ml, mixes, regulate pH=7.4 ~ 7.6, high pressure (100Pa) sterilizing 20 ~ 25 minutes at 120 ~ 125 DEG C, adds active nitrobacteria pulvis (Shanghai City Chong Guan commerce and trade company limited, Shanghai City) of 2 ~ 5g, ferment under aerobic conditions 48 ~ 72 hours, obtains nitrobacteria preparation stand-by.
Two, experiment grouping:
Control group: get live yeast bacteria preparation 1 part, bifidobacterium preparations 1 part, bacillus subtilis formulation 1 part, 1 part, active nitrobacteria preparation respectively as a control group;
Bigeminy bacterium group: select any two kinds of bacteria preparations by weight 1:1 carry out formula test, having 6 experimental group, is active yeast-bifidobacterium preparations, active yeast-bacillus subtilis formulation, active yeast-active nitrobacteria preparation, bifidus bacillus-bacillus subtilis formulation, bifidus bacillus-active nitrobacteria preparation, subtilis-active nitrobacteria preparation respectively;
Three bacterium groups: get live yeast bacteria preparation 33% respectively, bifidobacterium preparations 33%, bacillus subtilis formulation 33%, active nitrobacteria preparation 33%, the ratio uniform mixing of 1:1:1 pressed by any three kinds of preparations, regulate pH=6.5 ~ 7.0, obtain 4 kind of three bacterium probiotics formula, be respectively: active yeast, bifidus bacillus, active nitrobacteria preparation (1:1:1), bifidus bacillus, subtilis, active nitrobacteria (1:1:1) preparation, active yeast, bifidus bacillus, bacillus subtilis formulation (1:1:1), active yeast, subtilis, active nitrobacteria preparation (1:1:1),
Regulate pH=6.5 ~ 7.0;
Three, use the single microbial inoculum of 4 control groups, 6 bigeminy bacterium test group, 4 three bacterium mix bacterium agent to the fertilizer after just fermentation (after the chicken manure natural air drying that plant produces, the ratio adding moiety water in each weight part chicken manure adds water, be piled into raft, by plastic cloth sealing and fermenting 3 months) micro-oxygen conditions 25 DEG C fermentation 72 hours, the part by weight of microbial inoculum and fertilizer is 1:25.The fertilizer that 5 grams fermented being rendered to volume is in the cultivation water tank of 12 liters, at room temperature carries out planktonic organism (chlorella and the straight Magna of hook foot) and cultivates.Chlorella started to cultivate on November 13rd, 2014, and initial density is 2 × 10
6individual/liter, cultivate 50 days, when bead density reaches maximum (20 ~ 22 × 10
6individual/liter), and when substantially remaining unchanged, to add initial density be that the straight Magna of hook foot of 5/liter carries out cultivating (following scheme use same procedure) cultivating in water tank.
The cultivation results of control group and bigeminy bacterium test group as shown in figures 1 and 3, and shown in Dissolved Oxygen in Water and pH value variation diagram 2 and Fig. 4; Three bacterium results as shown in Figure 5, shown in Dissolved Oxygen in Water and pH value variation diagram 6.
Can find from the cultivation result of Fig. 1, Fig. 3 and Fig. 5 chlorella and the straight Magna of hook foot, when carrying out chlorella and the straight Magna cultural aim of hook foot, no matter single culture or strain combination fermentation fertilizer, all there is better effects to the cultivation of chlorella, but carry out hook foot straight Magna when cultivating, find that single culture carries out in the control group fermented, only this group of yeast has certain way to cultivate to the straight Magna of hook foot, when other three bacterial classifications are used alone, all effect be there is no to the cultivation of the straight Magna of hook foot.And in the test group of two kinds of strain combinations, only have three groups to hook foot straight Magna cultivation effective, be yeast and nitrobacteria test group, bifidus bacillus and subtilis test group, bifidus bacillus and nitrobacteria test group respectively.In the test group of three kinds of strain combinations, only the experimental group of yeast, bifidus bacillus, nitrobacteria (1:1:1) and bifidus bacillus, subtilis, the cultivation of nitrobacteria (1:1:1) to the straight Magna of hook foot have better effects.Illustrate that in these 5 test group the fertilizer of the strain combination fermentation passed through creates multi-cultur es synergy to the cultivation of the straight Magna of hook foot.
By contrast way to cultivate, can preferably bifidus bacillus and nitrobacteria (proportioning of 1:1) and bifidus bacillus, subtilis, nitrobacteria (proportioning of 1:1:1) as cultivating planktonic 2 kinds of Tiny ecosystem agent prescriptions in reservoir ecologic breeding.
In experiment initial stage, mid-term and latter stage, respectively dissolved oxygen and pH value mensuration are carried out to the water body of each experimental group, measurement result finds, no matter be the control group that single culture carries out fermenting, or two microorganisms coordinates the test group of carrying out fermenting, change from the test initial stage to test dissolved oxygen in latter stage and pH value has basically identical Changing Pattern: all show as dissolved oxygen and rise gradually, pH value declines gradually (Fig. 2, Fig. 4, Fig. 6).To testing latter stage, the dissolved oxygen content of all test group all meets the II class water quality standard of national water environment quality standard GB3838-2002 demarcation.Therefore the Tiny ecosystem agent prescription determined of the present invention in use, has good result to maintenance aquaculture water water quality.
In actual production in planktonic organism cultivating process, can every mu of water surface (average depth 6 meters) throw in the Tiny ecosystem reagent prepared in 1.5-2.0kg the invention process case ferment again after fertilizer, within every 30-40 days, throw in once, the planktonic organism of cultivation can omnidistancely use in reservoir ecological fishery cultivating.Use the probiotics in the present invention to cultivate zooplankton and carry out fish culture in reservoir, both can purify water, and improve aquaculture water environment, and fishery production can be improved largely again.
Claims (4)
1., for the probiotics that medium and small reservoirs ecologic breeding bait planktonic organism is cultivated, it is characterized in that comprising
Live yeast bacteria preparation; Or
Live yeast bacteria preparation and nitrobacteria preparation 1:1 mixture by weight; Bifidobacterium preparations and bacillus subtilis formulation be 1:1 mixture by weight; Bifidobacterium preparations and nitrobacteria preparation 1:1 mixture by weight; Or
Live yeast bacteria preparation, bifidobacterium preparations, nitrobacteria preparation 1:1:1 mixture by weight; Bifidobacterium preparations, bacillus subtilis formulation, nitrobacteria preparation 1:1:1 mixture by weight;
During use, by probiotics to the fertilizer after just fermentation according to weight ratio 1:25 ratio, micro-oxygen conditions 25 DEG C fermentation 72 hours the fertilizer that ferment again; The fertilizer that will ferment again is rendered to and is cultivated in water tank, and at room temperature carry out planktonic organism chlorella and the straight Magna cultivation of hook foot, injected volume is that every 5 grams of fertilizers that ferment again are rendered in 12 premium on currency.
2., for the fertilizer that medium and small reservoirs ecologic breeding bait planktonic organism is cultivated, its preparation method is as follows:
(1) probiotics is prepared:
Get live yeast bacteria preparation; Or
Live yeast bacteria preparation and nitrobacteria preparation 1:1 mixture by weight; Bifidobacterium preparations and bacillus subtilis formulation be 1:1 mixture by weight; Bifidobacterium preparations and nitrobacteria preparation 1:1 mixture by weight; Or
Live yeast bacteria preparation, bifidobacterium preparations, nitrobacteria preparation 1:1:1 mixture by weight; Bifidobacterium preparations, bacillus subtilis formulation, nitrobacteria preparation 1:1:1 mixture by weight;
(2) by probiotics to the fertilizer after just fermentation according to weight ratio 1:25 ratio, micro-oxygen conditions 25 DEG C fermentation 72 hours the fertilizer that ferment again.
3. the using method of fertilizer described in claim 2, is characterized in that: the fertilizer that will ferment again is rendered in 12 premium on currency according to the every 5 grams fertilizers that ferment again.
4. the probiotics cultivated for medium and small reservoirs ecologic breeding bait planktonic organism according to claim 1, is characterized in that:
The preparation of described live yeast bacteria preparation: get glucose 6 ~ 10g, peptone 3 ~ 5g, yeast extract paste 1.5 ~ 2.5g, distilled water 850 ~ 900ml, mix, autoclaving 20 ~ 25 minutes at 120 ~ 125 DEG C, adds 2 ~ 5g active yeast powder, the lower 25 DEG C of fermentations of anaerobic condition 72 ~ 96 hours, obtain live yeast bacteria preparation;
The preparation of described bifidobacterium preparations: get glucose 10 ~ 12g, peptone 7 ~ 9g, yeast extract paste powder 1 ~ 2g, Zulkovsky starch 0.2 ~ 0.3g, sodium-chlor 2 ~ 3g, halfcystine 0.2 ~ 0.3g, pork liver powder 0.2 ~ 0.3g, tween-80 0.4 ~ 0.6g, agar 7 ~ 9g, distilled water 600ml, tomato leach liquor 400ml, mixes, high pressure (100Pa) sterilizing 15 ~ 20 minutes at 120 ~ 125 DEG C, add 2 ~ 5g bifidobacteria viable bacteria kind, the lower 25 DEG C of fermentations of anaerobic condition 36 ~ 48 hours, obtain bifidobacterium preparations; Described tomato leach liquor preparation method: fresh tomato weighs cleans chopping, adds equivalent distilled water and heats in 100 DEG C of water-baths, stir 1.5 ~ 2 hours, by filtered through gauze, corrects pH=7;
The preparation of described bacillus subtilis formulation: get glucose 10 ~ 12g, peptone 7 ~ 9g, sodium-chlor 2 ~ 3g, extractum carnis 0.2 ~ 0.3g, agar 10 ~ 12g, distilled water 1000ml, mix, autoclaving 25 ~ 30 minutes at 120 ~ 125 DEG C, add the bacillus subtilis formulation of 2 ~ 5g activation, the lower 25 DEG C of fermentations of anaerobic condition 36 ~ 48 hours, obtain bacillus subtilis formulation;
The preparation of described nitrobacteria preparation: get ammonium sulfate 0.4 ~ 0.6g, sodium-chlor 0.2 ~ 0.4g, ferrous sulfate 0.02 ~ 0.04g, SODIUM PHOSPHATE, MONOBASIC 2 ~ 3g, magnesium sulfate 0.02 ~ 0.04g, calcium chloride 6 ~ 8g, agar 18 ~ 20g, distilled water 1000ml, mixes, and regulates pH=7.4 ~ 7.6, autoclaving 20 ~ 25 minutes at 120 ~ 125 DEG C, add the active nitrobacteria pulvis of 2 ~ 5g, ferment under aerobic conditions 48 ~ 72 hours, obtains nitrobacteria preparation.
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