CN102583776A - Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method - Google Patents
Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method Download PDFInfo
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Abstract
The invention relates to a compound bacteria enzyme preparation for improving a cultivation watery environment and a preparation method. The compound bacteria enzyme preparation comprises the following components by weight parts: 35-45 parts of active biological enzyme mixed powder, 25-30 parts of multifunctional microbial bacterium powder, and 25-40 parts of bentonite. All the components are fully and uniformly mixed and are loaded into a water-soluble end product bag, thereby forming the compound bacteria enzyme preparation. According to the preparation method, various active biological enzymes, multifunctional microbial bacterial strains and carriers are utilized, are reasonably compounded and are packaged in the water-soluble end product bags, and then the bags are directly put into the cultivation water, so that the macromolecular organic matters, such as protein and fat, in a cultivation environment are quickly degraded, the components harmful to cultivated organisms in the water and substrate environment are removed, the growth propagation of pathogenic microorganisms is restrained, the ecological environment of cultivation water is quickly repaired, the secondary pollution of water is prevented, an excellent watery environment is created for aquaculture organisms, and the output and the quality of the cultivated organisms are increased.
Description
Technical field
The present invention relates to a kind of composite bacteria zymin and preparation method who improves the aquaculture water environment, belong to technical field of aquaculture.
Background technology
Since the reform and opening-up, the culture fishery of China has obtained the development of advancing by leaps and bounds, and becomes the fishery big country that unique in the world cultured output surpasses fishery output.It is traditional aquaculture model of technical characterictic that the existing culture fishery of China has been acted on " close, mixed, wheel " mostly, promptly obtains the aquaculture model of high yield with many kinds, high-density, heavy dressing bait throwing in.In order to improve the cultured output of unit of water body, the high yield that covets often increases water nutrition excessively; Excessively bait throwing in, fertilising and increasing breeding density; Nitrogen, phosphorus surge in the causes water, transparency descends, water quality deterioration; The bait in the water body and the metabolite of breed variety accumulate in pond bottom in a large number, and substrate pollution is serious.Organic matter decomposition excessive in water body and the substrate not only can consume the dissolved oxygen in the water body; Make water body environment be in anoxic condition; Simultaneously can discharge many objectionable impuritiess,, endanger the aquaculture organism in the water body directly or indirectly like ammonia nitrogen, nitrite and hydrogen sulfide etc.; Make that species diversity descends in the aquaculture water, the breed of high-quality aquatic products kind receives the restriction of water quality and food organism.The water body natural, ecological is destroyed, and cultures the space aquatic animal disease and spreads, and the disease of aquatic animal takes place frequent, and fishery loss is huge.In order to prevent disease, in the whole culture-cycle, all using the fish medicine at present.Though the use of fish medicine has obtained the effect of emergence therapeutic, brought such as resistance more, in fishery products and environment, accumulated ecological consequences such as residual.Therefore from ecological angle, although aquaculture water is a very complicated aquatic ecosystem, no matter how complicated it is, mainly be the equilibrium relationship between hydrobiont-cause of disease-environment three, be a problem of environment after all.The control of aquaculture water ecotope has become the key issue of development China aquaculture.
The quality of aquaculture water water quality and bottom environment has determined the eco-environmental quality of aquaculture water.In aquaculture water, water quality is the visualize of water body environment quality, some soluble substances, gases affect water quality, and bottom environment is the basic of water body environment quality.Meta-bolites, the hard-degraded substance of most remaining bait, aquatic animal accumulate in water bottom in a large number, make substrate be in the anaerobic environment of a reductibility for a long time, and biotic population is main with anaerobion.Secular anaerobic environment makes objectionable constituent such as water bottom ammonia nitrogen, hydrogen sulfide, nitrite, methane produce in a large number; It can get in the water body through processes such as deposition-solubilizing reaction, water body stirring and exchanges once more; Cause the secondary pollution of water body; Cause a large amount of breedings of pathogenic micro-organism, so very important to the improvement of aquaculture system environment.Present aquaculture water improving of environment agent mainly is divided into chemistry and biological two big classes.Chemotype improving of environment agent effect is quick, and effect is directly perceived, and consumption is less, but effective object is single, and the time length is short, and often has ecological insecurity, cures the symptoms, not the disease; Biotype improving of environment agent is main, safe and effective with microbial preparation mainly, and lasting effect is better, but onset time is slow, large usage quantity, and effect receives environmental influence and instability.Therefore in the cultivation water environment improvement, be badly in need of a kind of novel water body environment modifying agent that integrates the advantage of chemotype and biotype improving of environment agent of exploitation, to satisfy the needs of industry development.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art; A kind of composite bacteria zymin and preparation method who improves the aquaculture water environment is provided, and it utilizes various active enzyme, multifunctional microbial bacterial strain and carrier, after rationally composite and employing water soluble package bag is packed, directly drops into aquaculture water; Larger molecular organics matter such as the protein in the breeding environment capable of being fast degraded, fat; To the deleterious composition of aquaculture organism, suppress the growth and breeding of pathogenic micro-organism in removal water quality and the bottom environment, repair the aquaculture water ecotope fast; Prevent secondary pollution of water, for aquaculture organisms is built a good water body environment.
According to technical scheme provided by the invention: a kind of composite bacteria zymin that improves the aquaculture water environment is characterized in that: comprise following component, its component ratio is counted by weight:
Get 35 ~ 45 parts of active biological enzyme mixed powders, 25 ~ 30 parts in multifunctional microbial bacterium powder, 25 ~ 40 parts of wilkinites, thorough mixing is even, and the water-soluble finished bag of packing into together is the composite bacteria zymin.
As further improvement of the present invention; Said active biological enzyme mixed powder comprises papoid, bromeline, neutral protease, glycase, lypase and cellulase; They are placed in the mixing machine mix, promptly process the active biological enzyme mixed powder.
As further improvement of the present invention, said multifunctional microbial bacterium powder comprises subtilis bacterium powder, yeast powder and lactobacillus powder, they is placed in the mixing machine mix, and promptly processes multifunctional microbial bacterium powder.
A kind of composite bacteria enzyme preparation method that improves the aquaculture water environment is characterized in that: adopt following process step:
(1), the preparation of active biological enzyme mixed powder:
Get 8 ~ 12 parts of 3 ~ 5 parts of 600,000 commercially available U/g papoids, 10 ~ 15 parts of 500,000 U/g bromelines, 50 ~ 60 parts of 50,000 U/g neutral proteases, 10 ~ 12 parts of 5000U/g glycase, 3 ~ 6 parts in 20,000 U/g lypase and 160,000 U/g cellulases; Be placed down in the mixing machine in 20 ℃ and mix; Mixing time is 2 ~ 5 minutes, makes the active biological enzyme mixed powder;
(2), the preparation of multifunctional microbial bacterium powder mixed powder:
Get 25 ~ 35 parts in 45 ~ 55 parts in subtilis bacterium powder, yeast powder and 20 ~ 25 parts of lactobacillus powders, be placed down in the mixing machine in 20 ℃ and mix, mixing time is 5 ~ 8 minutes, makes multifunctional microbial bacterium powder mixed powder;
(3), get 35 ~ 45 parts of the active biological enzyme mixed powders that step (1) makes; 25 ~ 30 parts of the multifunctional microbial bacterium powder mixed powders that step (2) makes; 25 ~ 40 parts of wilkinites; Be placed down in the mixing machine in 20 ℃ and mix, mixing time is 2 ~ 5 minutes, makes composite bacteria enzyme mixed powder; Adopt the water soluble package bag to be packaged into bag this composite bacteria enzyme mixed powder again, promptly make the composite bacteria zymin through automatic packing machine.
As said preparing method's further improvement, the order number of said subtilis bacterium powder is 80 orders, viable count>=10
8Individual/g; The order number of said yeast powder is 80 orders, viable count>=10
8Individual/g; The order number of said lactobacillus powder is 80 orders, viable count>=10
8Individual/g; Said bentonitic order number is 80 orders.
As said preparing method's further improvement, said water soluble package bag is the non-colored light face, from 15 ~ 35 ℃ of solubility temperatures, thickness 0.05mm, big or small 4cm * 6cm.
The action principle of each component is following among the present invention:
1, papoid: Papain is called for short papain, is called Fructus Chaenomelis ferment again, be utilize immature papaya (
Carica papaya) milk in the fruit, adopt modern biotechnology to refine the pure-natural biological enzyme prepn that forms.It is to dredge base (SH) endopeptidase has the activity of proteolytic enzyme and esterase, and specificity is more widely arranged, and animal/vegetable protein, polypeptide, ester, acid amides etc. are had stronger hydrolysis ability a kind of containing.Simultaneously, also have complex functionality, can synthesize proteinoid to proteolysate.Water-soluble and glycerine, the aqueous solution is colourless or faint yellow, is creamy white sometimes; Be dissolved in organic solvents such as ethanol, chloroform and ether hardly.The most suitable pH value 6～7 (general 3～9.5 all can), it is 8.75 that effect, iso-electric point are also arranged when neutrality or slant acidity; 55～65 ℃ of the most suitable temperature (general 10～85 ℃ all can), thermotolerance is strong, also can complete deactivation in the time of 90 ℃; Oxidated dose of inhibition, reducing substances activates.The present invention utilizes it to come protein and ester class in the quick water of decomposition environment.
2, bromeline: Bromelain is called for short Traumanase, also is called bromelin or pineapple ferment, is to extract from pineapple stem end, leaf, skin, through refining, purify, concentrate, enzyme immobilization, lyophilize and a kind of natural plant enzyme of obtaining.Its outward appearance is light grey Powdered, and molecular weight is 33000, and iso-electric point is 9.55.Water-soluble, the aqueous solution is colourless to have opalescence sometimes to faint yellow, is insoluble to ethanol, chloroform and ether, belongs to gp.Can proteinic macromole be hydrolyzed to small molecules amino acid and the protein that is prone to absorption.53 ± 1 ℃ of optimum tempss, deactivation temperature >=65 ℃, best pH value 5.0～8.0, inactivation pH value be less than 3.0 with big with 9.5.Can make the polypeptide class be hydrolyzed to low-molecular-weight peptide class, the effect of hydrolyzed amide groups key and ester class is still arranged.Product has been widely used in industries such as food, medicine.The present invention utilizes it to promote protein and ester class in the water of decomposition environment.
3, neutral protease: neutral protease is got through fermented extracted by subtilis, belongs to a kind of restriction endonuclease, can be used for the range protein hydrolysis treatment.Under certain temperature, pH value, utilize the enzymatic reaction of neutral protease, can be hydrolyzed into small-molecular peptides or amino acid to vegeto-animal macro-molecular protein, be beneficial to proteinic effective absorption and utilization.35 ~ 55 ℃ of hydrolysis temperatures, pH value 6.0 ~ 7.5.The present invention utilizes it to come the protein in residual bait in the hydrolysis water environment or the animal metabolism thing.
4, glycase: amylase, general action is in α-1 such as Zulkovsky starch, amylose starch, glycogen, 4-VISOSE, hydrolyzing alpha-1, the enzyme of 4-glycosidic link.Difference according to enzymic hydrolysate isomery type can be divided into AMS and beta-amylase.Glycase is the enzyme general name of hydrolyzed starch and glycogen, through the catalyzed by amylase hydrolyzed starch, has high efficiency and specificity usually.The present invention utilizes it to promote the carbohydrate macromole in the water of decomposition environment.
5, lypase: lypase is the triacyl glycerol Acyl-hydrolase; It is one type of enzyme with multiple catalytic capability; Can catalysis natural substrate grease be the reverse building-up reactions of hydrolysis, alcoholysis, esterification, transesterificationization and the ester class of triglyceride and some other water-insoluble ester classes, generate lipid acid, glycerine and monoglyceride or diester.In addition also show the activity of some other enzyme, like Phospholipid hydrolase, lysophospholipase, Sterol esterase, acylpetide hydrolase activity etc.The present invention utilizes it to promote the lipid macromole in the water of decomposition environment.
6, cellulase: cellulase is a kind of of enzyme, when decomposition of cellulose, plays biocatalysis.
Be the protein or the RNA that can Mierocrystalline cellulose be resolved into polysaccharide or monose.Cellulase extensively is present in the natural organism, can both produce cellulase in the bacterium, fungi, animal body etc.The cellulase that generally is used to produce comes from fungi, and wooden enzyme genus, Aspergillus and penicillium are more typically arranged.Cellulase is a kind of important enzyme product, is a kind of prozyme, mainly is made up of circumscribed beta-glucanase, inscribe beta-glucanase and beta-glucosidase etc., also has the Xylanase activity of very high vigor.The present invention utilizes it to come the Mierocrystalline cellulose in residual bait in the hydrolysis water environment or the animal metabolism thing.
7, subtilis bacterium powder: subtilis is a kind of of bacillus, single thalline 0.7～0.8 um * 2～3um, uniform coloring.No pod membrane, peritrichous can move.Gram-positive microorganism, gemma size are 0.6～0.9 um * 1.0～1.5um, and be oval to column, is positioned at thalline central authorities or inclined to one side slightly, and gemma forms the back thalline and do not expand.The bacterium colony surface irregularity is opaque, dirty white or little yellow.Subtilis bacterium powder is the fermentative prodn of subtilis, in the thalli growth process, can produce subtilyne, polymyxin, nystatin, linear gramicidins isoreactivity material, and the pathogenic micro-organism in the water surrounding is had the obvious suppression effect; Subtilis can stimulate aquatic animal to grow, and improves Tegeline and antibody horizontal, strengthens cellular immunization and humoral immune function, improves herd immunity; Subtilis thalline self synthesizes enzymes such as AMS, lypase, cellulase, in digestive tube, together plays a role with the intravital digestive enzymes of animal; Subtilis can absorb COD in the water, ammonia nitrogen, nitrite etc. in process of growth, have purifying water effect preferably.The present invention utilizes multiple functions such as it is antibacterial, water purification to repair water body environment.
8, yeast powder: select fine yeast bacterial strain for use, adopt the fermentation of unique biological engineering to form, be applied to the high-activity microorganism microbial inoculum of livestock and poultry and culture fishery, have characteristics such as degeneration-resistant environment is strong, survival rate is high, flexibility is strong.The yeast powder is the grey powder, fineness>=80 orders, moisture≤8%.Can effectively improve enteric microorganism flora ratio, promote the beneficial microorganism growth and breeding, repel the absorption field planting of pathogenic micro-organism on the intestines mucosa surface; Thalline rich in proteins and vitamin B group can provide rich in protein, nucleic acid, VITAMINs and plurality of enzymes, have rich nutrient contents, increase the feed palatability, promote the digest and assimilate ability of animal to feed; Be rich in mannooligo saccharide, can promote body's immunity, strengthen disease resistance; Can produce multiple sweet-smelling property material, can effectively remove stink, dispensing gets in the water body can effectively promote algae grows, and it is tender refreshing to keep water body to live.Seed is supported the phase temporarily, and anti-stress improves surviving rate more than 20%, and fast growth is energetic, neat specification, and physique is healthy and strong.The present invention utilizes multiple functions such as its deodorizing water purification, short long anti-stress to improve breeding ecological.
9, lactobacillus powder: milk-acid bacteria is meant that the fermenting carbohydrate primary product is one type of no gemma of lactic acid, the general name of gram-positive bacterium.Every bacterium that can from the fermenting process of glucose or lactose, produce lactic acid is referred to as milk-acid bacteria.Milk-acid bacteria can effectively be adjusted the colony balance of aquatic animal enteron aisle; Kill or suppress pathogenic micro-organism through toxin such as the competition of nutrient competition, attachment site or secretion microbiotic, bacteriocins; Strengthen anti-infection ability; Regulate the immunocompetence of body intestines mucosa, improve the survival rate of animal when suffering the disease invasion and attack; Milk-acid bacteria can secrete lactic acid, produces multiple digestive ferment, can help fish to digest and assimilate, and promotes growth; Milk-acid bacteria can promote that not only food organism breed in a large number, grow, and can decompose organic matter in the residual bait of fish, ight soil and the water, and the improvement water body environment suppresses the breeding growth of harmful bacterium in the water body, and the adjusting algae is balanced each other, the harmful bacterium algae of control.The present invention utilizes its multiple function to improve breeding ecological.
10, water soluble package bag: adopt water-solubility PVA to process; Have the self-dissolving of the water of chance, prevent directly to contact medicine; Avoid unnecessary drug damages, reduce safer, convenient when abandoning the environmental pollution, the accurate quantification that cause and use, reduce production and use cost, use because of packing material, air is had high barrier and oil-proofness, the dust of static electrification in can absorbed air hardly, improved the clean rate and the various advantages such as aesthetic property, slow-releasing of wrapped product.The present invention utilizes its multiple function to improve product shelf time and practical application.
Compared with present technology the present invention has the following advantages:
The present invention utilizes the harmless various active enzyme of aquaculture organisms, multifunctional microbial microbial inoculum and carrier; After rationally composite and employing water soluble package bag is packed, directly drop into aquaculture water; Larger molecular organics matter such as the protein in the breeding environment capable of being fast degraded, fat, glucide increase the water body oxygen content, remove in water quality and the bottom environment the deleterious composition of aquaculture organism; The growth and breeding that suppresses pathogenic micro-organism; Repair the aquaculture water ecotope fast, prevent secondary pollution of water, help the good water body environment of surviving and growing for one of aquaculture organisms construction.Composite bacteria zymin of the present invention adopts water soluble package bag packing, can direct related packing drop into water body environment together, has reduced pollution, and is easy to use, do not receive the influence of envrionment conditions, and usage quantity is little, and the consumption of every meter depth of water of every mu of water surface is merely 50 ~ 100g; Use back instant effect and effect stability, can significantly reduce content of harmful such as organic substance in the water body, ammonia nitrogen, nitrite, hydrogen sulfide, increase the water body dissolved oxygen; Reduce algae density; Enhance the transparency, improve the environmental quality of water quality and substrate, improve the aquaculture organism life condition; Effectively prevent aquaculture organism disease and sudden death, improve the output and the quality of aquaculture organism.
Embodiment
Below in conjunction with specific embodiment the present invention is described further.
Embodiment 1
A kind of composite bacteria zymin and preparation method who improves the aquaculture water environment, adopt following process step: its component ratio is counted by weight:
(1), the preparation of active biological enzyme mixed powder:
Get 12 parts of 3 parts of 600,000 commercially available U/g papoids, 12 parts of 500,000 U/g bromelines, 60 parts of 50,000 U/g neutral proteases, 12 parts of 5000U/g glycase, 6 parts in 20,000 U/g lypase and 160,000 U/g cellulases; Be placed down in the mixing machine in 20 ℃ and mix; Mixing time is 2 minutes, makes the active biological enzyme mixed powder;
(2), the preparation of multifunctional microbial bacterium powder mixed powder:
(the order number is 80 orders, viable count>=10 to get subtilis bacterium powder
8Individual/g) 50 parts, (the order number is 80 orders to the yeast powder, viable count>=10
8Individual/g) 25 parts and lactobacillus powder (the order number is 80 orders, viable count>=10
8Individual/g) 25 parts, be placed down in the mixing machine in 20 ℃ and mix, mixing time is 5 minutes, makes multifunctional microbial bacterium powder mixed powder;
(3), get 45 parts of the active biological enzyme mixed powders that step (1) makes; 30 parts in the multifunctional microbial bacterium powder that step (2) makes, 25 parts of 80 order wilkinites are placed down in the mixing machine in 20 ℃ and mix; Mixing time is 5 minutes, makes composite bacteria enzyme mixed powder; Again with this composite bacteria enzyme mixed powder adopt water soluble package bag (the non-colored light face, from 15 ~ 35 ℃ of solubility temperatures, thickness 0.05mm, big or small 4cm * 6cm) be packaged into bag (a 200g/ bag) through automatic packing machine promptly makes the composite bacteria zymin.
The result of use of this composite bacteria zymin is following:
A certain Penaeus vannamei cultivating pool uses in Shanghai; 15 mu of areas, summer high temperature is the pond water quality overrich when (6～August), and water colour is bad; Ammonia nitrogen, nitrite, hydrogen sulfide etc. reach 1.85mg/L, 0.65mg/L and 0.32mg/L respectively in the water quality, and it is serious to exceed standard.The substrate blackout is smelly, and transparency is merely 15cm, influences the growth of Penaeus vannamei, and " the commentaries on classics pool " phenomenon and the disease of shrimp are serious, can detect vibrios on the shrimp body.One morning 10 name a person for a particular job above-mentioned prescription the composite bacteria zymin be used for culturing the pond, consumption is 80g/ mu Mi Shuishen.Greatly take a turn for the better with half water colour one day after, transfer oyster to by strong green, transparency increases to 27cm; The substrate stink disappears; Ammonia nitrogen, nitrite and hydrogen sulfide content are reduced to 0.74mg/L, 0.20mg/L and 0.10mg/L in the water body, and vibrios significantly reduces on the shrimp body, and shrimps are movable to recover normal.
Embodiment 2
A kind of composite bacteria zymin and preparation method who improves the aquaculture water environment, adopt following process step: its component ratio is counted by weight:
(1), the preparation of active biological enzyme mixed powder:
Get 10 parts of 5 parts of 600,000 commercially available U/g papoids, 10 parts of 500,000 U/g bromelines, 60 parts of 50,000 U/g neutral proteases, 10 parts of 5000U/g glycase, 5 parts in 20,000 U/g lypase and 160,000 U/g cellulases; Be placed down in the mixing machine in 20 ℃ and mix; Mixing time is 3 minutes, makes the active biological enzyme mixed powder;
(2), the preparation of multifunctional microbial bacterium powder mixed powder:
(the order number is 80 orders, viable count>=10 to get subtilis bacterium powder
8Individual/g) 45 parts, (the order number is 80 orders to the yeast powder, viable count>=10
8Individual/g) 30 parts and lactobacillus powder (the order number is 80 orders, viable count>=10
8Individual/g) 25 parts, be placed down in the mixing machine in 20 ℃ and mix, mixing time is 6 minutes, makes multifunctional microbial bacterium powder mixed powder;
(3), get 40 parts of the active biological enzyme mixed powders that step (1) makes; 30 parts in the multifunctional microbial bacterium powder that step (2) makes, 30 parts of 80 order wilkinites are placed down in the mixing machine in 20 ℃ and mix; Mixing time is 4 minutes, makes composite bacteria enzyme mixed powder; Again with this composite bacteria enzyme mixed powder adopt water soluble package bag (the non-colored light face, from 15 ~ 35 ℃ of solubility temperatures, thickness 0.05mm, big or small 4cm * 6cm) be packaged into bag (a 200g/ bag) through automatic packing machine promptly makes the composite bacteria zymin.
The result of use of this composite bacteria zymin is following:
A certain Macrobrachium rosenbergii cultivating pool uses in Gaoyou, Jiangsu; 10 mu of areas, summer high temperature is the pond water quality overrich when (6～July), and water colour is bad; Ammonia nitrogen, nitrite, hydrogen sulfide etc. reach 1.29mg/L, 0.43mg/L and 0.28mg/L respectively in the water quality, and it is serious to exceed standard.The substrate blackout is smelly, and transparency is merely 18cm, influences the growth of Macrobrachium rosenbergii, and " the commentaries on classics pool " phenomenon and the disease of shrimp are serious.One morning 10 name a person for a particular job above-mentioned prescription the composite bacteria zymin be used for culturing the pond, consumption is 50g/mu Mi Shuishen.Greatly take a turn for the better with water colour one day after, transfer light green to by blue-greenish colour, transparency increases to 28cm, and the substrate stink disappears, and ammonia nitrogen, nitrite and hydrogen sulfide content are reduced to 0.63mg/L, 0.16mg/L and 0.10mg/L in the water body, and shrimps are movable to recover normal.
Embodiment 3
A kind of composite bacteria zymin and preparation method who improves the aquaculture water environment, adopt following process step: its component ratio is counted by weight:
(1), the preparation of active biological enzyme mixed powder:
Get 10 parts of 5 parts of 600,000 commercially available U/g papoids, 15 parts of 500,000 U/g bromelines, 55 parts of 50,000 U/g neutral proteases, 10 parts of 5000U/g glycase, 5 parts in 20,000 U/g lypase and 160,000 U/g cellulases; Be placed down in the mixing machine in 20 ℃ and mix; Mixing time is 4 minutes, makes the active biological enzyme mixed powder;
(2), the preparation of multifunctional microbial bacterium powder mixed powder:
(the order number is 80 orders, viable count>=10 to get subtilis bacterium powder
8Individual/g) 48 parts, (the order number is 80 orders to the yeast powder, viable count>=10
8Individual/g) 32 parts and lactobacillus powder (the order number is 80 orders, viable count>=10
8Individual/g) 20 parts, be placed down in the mixing machine in 20 ℃ and mix, mixing time is 8 minutes, makes multifunctional microbial bacterium powder mixed powder;
(3), get 42 parts of the active biological enzyme mixed powders that step (1) makes; 28 parts in the multifunctional microbial bacterium powder that step (2) makes, 30 parts of 80 order wilkinites are placed down in the mixing machine in 20 ℃ and mix; Mixing time is 2 minutes, makes composite bacteria enzyme mixed powder; Again with this composite bacteria enzyme mixed powder adopt water soluble package bag (the non-colored light face, from 15 ~ 35 ℃ of solubility temperatures, thickness 0.05mm, big or small 4cm * 6cm) be packaged into bag (a 200g/ bag) through automatic packing machine promptly makes the composite bacteria zymin.
The result of use of this composite bacteria zymin is following:
A mandarin fish cultivating pool uses in somewhere, Huzhou, Zhejiang, and area is 10 mu, because of a large amount of the throwing raised the bright forage fiss of ice; Cause high temperature season pond water quality overrich; Water colour is bad, and ammonia nitrogen, nitrite, hydrogen sulfide etc. reach 1.82mg/L, 0.59mg/L and 0.42mg/L respectively in the water quality, and it is serious to exceed standard.The substrate blackout is smelly, influences the growth of mandarin fish, and disease is serious, detects in the water body to have a liking for the water-based Aeromonas.One morning 10 name a person for a particular job above-mentioned prescription the composite bacteria zymin be used for culturing the pond, consumption is 100 g/ mu Mi Shuishen.Water colour with back 2 days ponds promptly greatly takes a turn for the better; Transparency increases to 28cm, and the substrate stink disappears, and ammonia nitrogen, nitrite and hydrogen sulfide content are reduced to 0.66mg/L, 0.17mg/L and 0.14mg/L in the water body; Have a liking for the water-based Aeromonas and do not detect, it is normal that fish movement recovers.
Among the present invention, described mixing machine, automatic packing machine etc. are conventional equipment, can on market, buy, and do not have any particular requirement, adopt common working method.The material that the present invention adopted is convenient sources also, all can on market, buy.
Claims (6)
1. composite bacteria zymin that improves the aquaculture water environment, it is characterized in that: comprise following component, its component ratio is counted by weight:
Get 35 ~ 45 parts of active biological enzyme mixed powders, 25 ~ 30 parts in multifunctional microbial bacterium powder, 25 ~ 40 parts of wilkinites, thorough mixing is even, and the water-soluble finished bag of packing into together is the composite bacteria zymin.
2. the composite bacteria zymin of improvement aquaculture water environment as claimed in claim 1; It is characterized in that: said active biological enzyme mixed powder comprises papoid, bromeline, neutral protease, glycase, lypase and cellulase; They are placed in the mixing machine mix, promptly process the active biological enzyme mixed powder.
3. the composite bacteria zymin of improvement aquaculture water environment as claimed in claim 1; It is characterized in that: said multifunctional microbial bacterium powder comprises subtilis bacterium powder, yeast powder and lactobacillus powder; They are placed in the mixing machine mix, promptly process multifunctional microbial bacterium powder.
4. composite bacteria enzyme preparation method that improves the aquaculture water environment is characterized in that: adopt following process step:
(1), the preparation of active biological enzyme mixed powder:
Get 8 ~ 12 parts of 3 ~ 5 parts of 600,000 commercially available U/g papoids, 10 ~ 15 parts of 500,000 U/g bromelines, 50 ~ 60 parts of 50,000 U/g neutral proteases, 10 ~ 12 parts of 5000U/g glycase, 3 ~ 6 parts in 20,000 U/g lypase and 160,000 U/g cellulases; Be placed down in the mixing machine in 20 ℃ and mix; Mixing time is 2 ~ 5 minutes, makes the active biological enzyme mixed powder;
(2), the preparation of multifunctional microbial bacterium powder mixed powder:
Get 25 ~ 35 parts in 45 ~ 55 parts in subtilis bacterium powder, yeast powder and 20 ~ 25 parts of lactobacillus powders, be placed down in the mixing machine in 20 ℃ and mix, mixing time is 5 ~ 8 minutes, makes multifunctional microbial bacterium powder mixed powder;
(3), get 35 ~ 45 parts of the active biological enzyme mixed powders that step (1) makes; 25 ~ 30 parts of the multifunctional microbial bacterium powder mixed powders that step (2) makes; 25 ~ 40 parts of wilkinites; Be placed down in the mixing machine in 20 ℃ and mix, mixing time is 2 ~ 5 minutes, makes composite bacteria enzyme mixed powder; Adopt the water soluble package bag to be packaged into bag this composite bacteria enzyme mixed powder again, promptly make the composite bacteria zymin through automatic packing machine.
5. the composite bacteria enzyme preparation method of improvement aquaculture water environment as claimed in claim 4 is characterized in that: the order number of said subtilis bacterium powder is 80 orders, viable count>=10
8Individual/g; The order number of said yeast powder is 80 orders, viable count>=10
8Individual/g; The order number of said lactobacillus powder is 80 orders, viable count>=10
8Individual/g; Said bentonitic order number is 80 orders.
6. the composite bacteria enzyme preparation method of improvement aquaculture water environment as claimed in claim 4 is characterized in that: said water soluble package bag is the non-colored light face, from 15 ~ 35 ℃ of solubility temperatures, thickness 0.05mm, big or small 4cm * 6cm.
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Cited By (28)
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| CN103058389A (en) * | 2012-12-31 | 2013-04-24 | 天津北洋百川生物技术有限公司 | In-situ purifying dry powder preparation for polluted water sludge |
| CN103466732A (en) * | 2013-10-12 | 2013-12-25 | 南京御水科技有限公司 | Safe and convenient water treatment agent addition and application method |
| CN104247891A (en) * | 2014-09-26 | 2014-12-31 | 浙江海洋学院 | Method for preventing hemorrhagic lernaea disease of crucian |
| CN104710020A (en) * | 2015-03-10 | 2015-06-17 | 北京麦克安吉生物技术有限公司 | Ecological enzyme substance capable of quickly improving density and degradation efficiency of beneficial bacteria in reaction environment |
| CN105124249A (en) * | 2015-08-31 | 2015-12-09 | 蚌埠龙达农业专业合作社 | Aquatic product mixed culturing feed capable of self degradation and preparation method of aquatic product mixed culturing feed |
| CN105254036A (en) * | 2015-11-24 | 2016-01-20 | 济南昊泽环保科技有限公司 | Hot-pickled mustard tuber production wastewater treating agent and wastewater treating method |
| CN105253969A (en) * | 2015-08-31 | 2016-01-20 | 蚌埠龙达农业专业合作社 | Contamination-reducing nutrient-increasing additive for aquaculture water and preparation method thereof |
| CN105585098A (en) * | 2015-12-30 | 2016-05-18 | 苏州市相城区太平蟹业专业合作社 | Water quality regulator and preparation method thereof |
| CN105779331A (en) * | 2016-03-07 | 2016-07-20 | 重庆坤泉环境工程有限公司 | Biological strain nutritional agent and preparation method and application thereof |
| CN106186347A (en) * | 2016-07-12 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of used in mariculture desulfurated plaster and preparation method thereof |
| CN107118983A (en) * | 2017-05-08 | 2017-09-01 | 中国海洋大学 | A kind of compound bacterial enzyme preparation for improving water quality and its preparation method and application |
| CN107140744A (en) * | 2017-04-21 | 2017-09-08 | 防城港市润杰生物科技有限公司 | A kind of microbial fermentation technology prepares the method that aquaculture water administers agent admittedly |
| CN107307002A (en) * | 2017-07-19 | 2017-11-03 | 上海地天生物科技有限公司 | A kind of Bacillus antidote |
| CN107307001A (en) * | 2017-06-27 | 2017-11-03 | 句容市北山水库管理所 | A kind of knife long-tailed anchovy cultivation water conditioning agent and preparation method thereof |
| CN107324609A (en) * | 2017-08-25 | 2017-11-07 | 侯晓明 | It is a kind of to improve the method for culture environment of aquatic products |
| CN107381832A (en) * | 2017-07-31 | 2017-11-24 | 浦江县协盈动物饲料技术开发有限公司 | The purposes of biological water purification agent |
| CN107434303A (en) * | 2017-07-31 | 2017-12-05 | 兰溪市普润斯水产养殖技术有限公司 | The processing method of eutrophication aquiculture waste water |
| CN107494994A (en) * | 2017-08-24 | 2017-12-22 | 无为县慧富水蛭养殖专业合作社 | A kind of leech pond cultivation fertilizer and preparation method thereof |
| CN107986452A (en) * | 2017-11-29 | 2018-05-04 | 深圳地天国际生物工程科技有限公司 | A kind of catabolic enzyme original powder and preparation method thereof |
| CN108522943A (en) * | 2017-03-06 | 2018-09-14 | 耿胜利 | A kind of preparation method for the bait antistaling agent cultivated using biotechnology |
| CN108813223A (en) * | 2018-07-03 | 2018-11-16 | 珠海市西科水产养殖有限公司 | A kind of aquaculture specific complex microorganism formulation and preparation method thereof |
| CN109748399A (en) * | 2019-03-15 | 2019-05-14 | 宁夏赛诺杰乙征农牧科技有限公司 | A kind of decontamination cycle of shrimp ponds tail water utilizes method |
| CN110313454A (en) * | 2018-03-28 | 2019-10-11 | 湖北龙王恨渔具集团有限公司 | A kind of bait nest material and preparation method thereof using compound bacteria microbial fermentation cereal crops |
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| CN110685020A (en) * | 2019-11-11 | 2020-01-14 | 南宁雄晋生物科技有限公司 | Complex enzyme for degumming and color fixation of natural colored silk and use method thereof |
| CN111573851A (en) * | 2020-05-21 | 2020-08-25 | 郑州慧泽生化科技有限公司 | Microorganism water regulating and purifying agent and preparation method thereof |
| CN112678968A (en) * | 2020-12-24 | 2021-04-20 | 广东莞绿环保工程有限公司 | Efficient microbial preparation for repairing black and odorous water body and bottom mud and preparation method thereof |
| CN113115672A (en) * | 2021-04-12 | 2021-07-16 | 吉林农业科技学院 | Method for preventing and controlling underground pests based on water-soluble membrane-released mite and bacterium complex |
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Cited By (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103058389B (en) * | 2012-12-31 | 2015-01-07 | 天津北洋百川生物技术有限公司 | In-situ purifying dry powder preparation for polluted water sludge |
| CN103058389A (en) * | 2012-12-31 | 2013-04-24 | 天津北洋百川生物技术有限公司 | In-situ purifying dry powder preparation for polluted water sludge |
| CN103466732A (en) * | 2013-10-12 | 2013-12-25 | 南京御水科技有限公司 | Safe and convenient water treatment agent addition and application method |
| CN104247891A (en) * | 2014-09-26 | 2014-12-31 | 浙江海洋学院 | Method for preventing hemorrhagic lernaea disease of crucian |
| CN104710020B (en) * | 2015-03-10 | 2016-08-17 | 北京科益创新环境技术有限公司 | The ecological enzyme matter of reaction environment probiotics density and degradation efficiency can be improved rapidly |
| CN104710020A (en) * | 2015-03-10 | 2015-06-17 | 北京麦克安吉生物技术有限公司 | Ecological enzyme substance capable of quickly improving density and degradation efficiency of beneficial bacteria in reaction environment |
| CN105124249A (en) * | 2015-08-31 | 2015-12-09 | 蚌埠龙达农业专业合作社 | Aquatic product mixed culturing feed capable of self degradation and preparation method of aquatic product mixed culturing feed |
| CN105253969A (en) * | 2015-08-31 | 2016-01-20 | 蚌埠龙达农业专业合作社 | Contamination-reducing nutrient-increasing additive for aquaculture water and preparation method thereof |
| CN105254036A (en) * | 2015-11-24 | 2016-01-20 | 济南昊泽环保科技有限公司 | Hot-pickled mustard tuber production wastewater treating agent and wastewater treating method |
| CN105585098A (en) * | 2015-12-30 | 2016-05-18 | 苏州市相城区太平蟹业专业合作社 | Water quality regulator and preparation method thereof |
| CN105779331A (en) * | 2016-03-07 | 2016-07-20 | 重庆坤泉环境工程有限公司 | Biological strain nutritional agent and preparation method and application thereof |
| CN106186347A (en) * | 2016-07-12 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of used in mariculture desulfurated plaster and preparation method thereof |
| CN108522943B (en) * | 2017-03-06 | 2021-05-07 | 耿胜利 | Preparation method of bait preservative cultivated by adopting bioengineering technology |
| CN108522943A (en) * | 2017-03-06 | 2018-09-14 | 耿胜利 | A kind of preparation method for the bait antistaling agent cultivated using biotechnology |
| CN107140744A (en) * | 2017-04-21 | 2017-09-08 | 防城港市润杰生物科技有限公司 | A kind of microbial fermentation technology prepares the method that aquaculture water administers agent admittedly |
| CN107118983A (en) * | 2017-05-08 | 2017-09-01 | 中国海洋大学 | A kind of compound bacterial enzyme preparation for improving water quality and its preparation method and application |
| CN107118983B (en) * | 2017-05-08 | 2021-07-06 | 中国海洋大学 | Compound bacterium enzyme preparation for improving water quality and preparation method and application thereof |
| CN107307001A (en) * | 2017-06-27 | 2017-11-03 | 句容市北山水库管理所 | A kind of knife long-tailed anchovy cultivation water conditioning agent and preparation method thereof |
| CN107307002A (en) * | 2017-07-19 | 2017-11-03 | 上海地天生物科技有限公司 | A kind of Bacillus antidote |
| CN107381832A (en) * | 2017-07-31 | 2017-11-24 | 浦江县协盈动物饲料技术开发有限公司 | The purposes of biological water purification agent |
| CN107434303A (en) * | 2017-07-31 | 2017-12-05 | 兰溪市普润斯水产养殖技术有限公司 | The processing method of eutrophication aquiculture waste water |
| CN107494994A (en) * | 2017-08-24 | 2017-12-22 | 无为县慧富水蛭养殖专业合作社 | A kind of leech pond cultivation fertilizer and preparation method thereof |
| CN107324609A (en) * | 2017-08-25 | 2017-11-07 | 侯晓明 | It is a kind of to improve the method for culture environment of aquatic products |
| CN107324609B (en) * | 2017-08-25 | 2020-07-14 | 海南省昌江南疆生物技术有限公司 | Method for improving aquaculture environment |
| CN107986452A (en) * | 2017-11-29 | 2018-05-04 | 深圳地天国际生物工程科技有限公司 | A kind of catabolic enzyme original powder and preparation method thereof |
| CN110313454A (en) * | 2018-03-28 | 2019-10-11 | 湖北龙王恨渔具集团有限公司 | A kind of bait nest material and preparation method thereof using compound bacteria microbial fermentation cereal crops |
| CN108813223A (en) * | 2018-07-03 | 2018-11-16 | 珠海市西科水产养殖有限公司 | A kind of aquaculture specific complex microorganism formulation and preparation method thereof |
| CN109748399A (en) * | 2019-03-15 | 2019-05-14 | 宁夏赛诺杰乙征农牧科技有限公司 | A kind of decontamination cycle of shrimp ponds tail water utilizes method |
| CN110606515A (en) * | 2019-08-27 | 2019-12-24 | 河海大学 | NH for removing sewage4+-N environment-friendly enzyme preparation and preparation method thereof |
| CN110685020A (en) * | 2019-11-11 | 2020-01-14 | 南宁雄晋生物科技有限公司 | Complex enzyme for degumming and color fixation of natural colored silk and use method thereof |
| CN110685020B (en) * | 2019-11-11 | 2020-09-04 | 南宁雄晋生物科技有限公司 | Complex enzyme for degumming and color fixation of natural colored silk and use method thereof |
| CN111573851A (en) * | 2020-05-21 | 2020-08-25 | 郑州慧泽生化科技有限公司 | Microorganism water regulating and purifying agent and preparation method thereof |
| CN112678968A (en) * | 2020-12-24 | 2021-04-20 | 广东莞绿环保工程有限公司 | Efficient microbial preparation for repairing black and odorous water body and bottom mud and preparation method thereof |
| CN113115672A (en) * | 2021-04-12 | 2021-07-16 | 吉林农业科技学院 | Method for preventing and controlling underground pests based on water-soluble membrane-released mite and bacterium complex |
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