CN1594551A - Micro-ecological bacteria preparation for rebuilding aquaculture water environment and method for preparing same - Google Patents
Micro-ecological bacteria preparation for rebuilding aquaculture water environment and method for preparing same Download PDFInfo
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- CN1594551A CN1594551A CN 200410019818 CN200410019818A CN1594551A CN 1594551 A CN1594551 A CN 1594551A CN 200410019818 CN200410019818 CN 200410019818 CN 200410019818 A CN200410019818 A CN 200410019818A CN 1594551 A CN1594551 A CN 1594551A
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- actinomycetes
- acid bacteria
- genus bacillus
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Abstract
The invention discloses a micro ecological preparation, more specificly a micro ecological preparation for breeding aquatic water environment recovery and its preparation method. Micro ecological preparations for rehabilitating breeding water are composition of bacillus, rayungus and lactobacillus, in which weight percent is : bacillus 60-80%, rayungus 10-20%, lactobacillus 10-20%. micro ecological preparations production method are as follows : strain activation and culture, liquid magnifying culture, fixing strain to wheat bran and proceeding solid culture. The inventive micro ecological preparations has merit of good adaptability, good breeding water rehabilitation effect, high livability.
Description
Technical field
The present invention relates to a kind of micro-ecological bacterial preparation, specifically a kind of micro-ecological bacterial preparation that is used for the cultivation water environment reparation and preparation method thereof.
Background technology
The present culture fishery of China, particularly the shrimp farming development is faced with water pollution, and aquatic ecosystem is unbalance, and to cause prawn disease to spread situation serious.Along with the raising and the plan of national development pollution-free food of people's living standard, carry out the prevention and cure of shrimp disease current requirement of incompatibility to heath food with antibiotic medicine.
Fresh water and sea farming great majority adopt the mode of " sealing semiclosed internal recycle cultures " (the hydrostatic sealing is cultured) at present, promptly do not change water culturing in season, therefore cause material bait and the excremental accumulation of aquaculture organism, summer, sweltering heat made water quality deterioration in addition, fishes and shrimps sickness rate height.In order to reduce disease loss, have to use in a large number microbiotic and chemicals, therefore further polluted water again causes fish and shrimp diseases to increase, the formation vicious cycle.Traditional breeding way not only makes the raising fish and shrimp cost up, and has a strong impact on the quality of fishes and shrimps, and sewage emissions causes serious environmental to pollute simultaneously.All seeking to improve water quality environment both at home and abroad, the effective ways of prevention fishes and shrimps disease.
Summary of the invention
Technical problem to be solved by this invention is: the micro-ecological bacterial preparation and preparation method thereof that the reparation cultivation water environment of a kind of environmental protection, high density and activity is provided.
The present invention solves this technical problem the technical scheme that is adopted:
The composition that the micro-ecological bacterial preparation of reparation cultivation water environment is made up of main pharmacodynamics composition genus bacillus, actinomycetes, the milk-acid bacteria of sterilant, wherein the weight percent of each component is: genus bacillus 60～80%, actinomycetes 10～20%, milk-acid bacteria 10～20%.
The preparation method who repairs the micro-ecological bacterial preparation of cultivation water environment comprises the steps:
1) each slant strains activation, shake-flask culture respectively of preserving.Genus bacillus and actinomycetes are used the Zulkovsky starch culture medium culturing, pH7.0～7.5,100～140 ℃ autoclaving 10-30 minute; Milk-acid bacteria is directly cultivated with the sterilization fresh cow milk; Genus bacillus was cultivated 20～24 hours for 35～40 ℃; Actinomycetes and milk-acid bacteria were cultivated 48～50 hours for 28～30 ℃.
2) spore bacillus, the level liquid enlarged culturing of actinomycetes and milk-acid bacteria (20L jar); Inoculum size 10%～20%.
3) genus bacillus, the secondary fluid enlargement culture of actinomycetes and milk-acid bacteria (200L jar); Inoculum size 10%～20%.
4) with wheat bran be carrier solid enlarged culturing and being immobilized onto on the wheat bran respectively.
5) genus bacillus, actinomycetes, milk-acid bacteria is dry below 10～40 ℃, pulverizing.
6) genus bacillus, actinomycetes, milk-acid bacteria are mixed, the weight percent of each component is: genus bacillus 60～80%, actinomycetes 10～20%, milk-acid bacteria 10～20%.
Beneficial effect of the present invention
1) composite bacteria is immobilized onto on the wheat bran, makes the living bacteria count (CFU) of every gram composite bacteria preparation almost reach most probable number MPN up to 10,000 hundred million, yet there are no the report of high bacteria containing amount bacteria preparation like this so far.Wheat bran can not only provide some nutritive ingredients by being cultivated bacterium, also because its reticulated structure has bigger specific surface area, so it is bigger to adhere to adsorption of immobilization in unit volume.
2) free NH
3And NO
2 -Being the main objectionable constituent of poisoning fishes and shrimps, also is to cause the shrimp main causes of death.If can reduce NH
4 +-N, free NH
3Also will reduce NO
2 --N also can reduce, and the pH value also just tends towards stability.Under the same dosage of similarity condition, we compare with the Bio-culture Aq (Bio-t) of Brookside company by the product (XIJ) of development, reduce NO
2 -The ability of the thalline N of-amount is more or less the same, but reduces NH
4 +The ability of-N but obviously is better than Bio-t.Being spilled into water body after bacteria preparation is activated can the little ecological dominance bacterium of very fast formation, can obviously reduce the COD and the nitrite nitrogen of culturing water, can stablize ammonia-state nitrogen (NH
4 +-N) and remain on the certain level.Make the pH value stabilization within the specific limits.
Positively effect of the present invention also is:
1. bacteria preparation of the present invention is activated, can the little ecological dominance flora of very fast formation in water, and the effect of bring into play its degradation of organic substances, shortly give birth to, benefit being given birth to.
2. can reduce COD rapidly;
3. reduction nitrite nitrogen;
4. ammonia-state nitrogen and pH value are remained on the certain level;
5. suppress the breeding of harmful germ, regulate algae density in the water, promote aquatic ecosystem reparation and balance;
6. promote shrimp gi tract health compared with the control, the control cost reduces by 80%, and shrimp output improves nearly 1 times, and has significantly improved the quality of shrimp.
7. replace or minimizing use microbiotic and chemicals, make shrimp farming become the nuisanceless green aquaculture.
8. it is strong that micro-ecological bacterial preparation of the present invention has adaptability, good to culturing the water ecological environment repairing effect, characteristics such as viable count height.
Embodiment
Embodiment 1
Follow these steps to carry out:
1) each slant strains with 4 ℃ of preservations activates, and the difference shake-flask culture (200ml～2L).Genus bacillus and actinomycetes are used the Zulkovsky starch culture medium culturing, and it is as follows specifically to fill a prescription: Zulkovsky starch 20g, KNO
31g, NaCl 0.5g, K
2HPO
40.5g, MgSO
4.7H
2O 0.5g, FeSO
40.01g, agar 15～20g, distilled water 1000ml.pH7.2～7.4,121 ℃ autoclaving 20 minutes.Milk-acid bacteria is directly cultivated with the sterilization fresh cow milk.Genus bacillus was cultivated 20～24 hours for 37 ℃; Actinomycetes and milk-acid bacteria were cultivated 48～50 hours for 28～30 ℃.
2) level liquid enlarged culturing (substratum and culture condition are the same for genus bacillus, actinomycetes and the independent enlarged culturing of milk-acid bacteria difference) (20L jar); Inoculum size 10%.
3) secondary liquid difference enlarged culturing (200L jar); Inoculum size 10%.
4) with wheat bran be carrier solid enlarged culturing and being immobilized onto on the wheat bran respectively.
5), be by the weight percent of each component with 25 ℃ of dryings of above-mentioned three class bacterium, pulverizing: genus bacillus 600 grams, actinomycetes 100 grams, milk-acid bacteria 100 grams mix, and after the assay was approved, are sealed in polyethylene bag, room temperature preservation.
Claims (2)
1. micro-ecological bacterial preparation of repairing cultivation water environment, it is characterized in that: the composition of forming by main pharmacodynamics composition genus bacillus, actinomycetes, the milk-acid bacteria of sterilant, wherein the weight percent of each component is: genus bacillus 60～80%, actinomycetes 10～20%, milk-acid bacteria 10～20%.
2. the preparation method of the micro-ecological bacterial preparation of reparation cultivation water environment as claimed in claim 1 is characterized in that it comprises the steps:
1) each slant strains activation, shake-flask culture respectively of preserving.Genus bacillus and actinomycetes are used the Zulkovsky starch culture medium culturing, pH7.0～7.5,100～140 ℃ autoclaving 10-30 minute; Milk-acid bacteria is directly cultivated with the sterilization fresh cow milk; Genus bacillus was cultivated 20～24 hours for 35～40 ℃; Actinomycetes and milk-acid bacteria were cultivated 48～50 hours for 28～30 ℃.
2) spore bacillus, the level liquid enlarged culturing of actinomycetes and milk-acid bacteria (20L jar); Inoculum size 10%～20%.
3) genus bacillus, the secondary fluid enlargement culture of actinomycetes and milk-acid bacteria (200L jar); Inoculum size 10%～20%.
4) with wheat bran be carrier solid enlarged culturing and being immobilized onto on the wheat bran respectively.
5) genus bacillus, actinomycetes, milk-acid bacteria is dry below 10～40 ℃, pulverizing.
6) genus bacillus, actinomycetes, milk-acid bacteria are mixed, the weight percent of each component is: genus bacillus 60～80%, actinomycetes 10～20%, milk-acid bacteria 10～20%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410019818 CN1594551A (en) | 2004-06-29 | 2004-06-29 | Micro-ecological bacteria preparation for rebuilding aquaculture water environment and method for preparing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410019818 CN1594551A (en) | 2004-06-29 | 2004-06-29 | Micro-ecological bacteria preparation for rebuilding aquaculture water environment and method for preparing same |
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| Publication Number | Publication Date |
|---|---|
| CN1594551A true CN1594551A (en) | 2005-03-16 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100374547C (en) * | 2005-12-28 | 2008-03-12 | 农业部环境保护科研监测所 | Composite bacillus subtilis and lactic acid bacteria microbe formulation preparation method |
| CN100441679C (en) * | 2006-11-24 | 2008-12-10 | 西安利能科技有限责任公司 | Bacterium composite nutrient |
| CN102583907A (en) * | 2012-03-21 | 2012-07-18 | 湖南富利来环保科技工程有限公司 | Organic sewage treatment system |
| CN102583776A (en) * | 2012-02-29 | 2012-07-18 | 中国水产科学研究院淡水渔业研究中心 | Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method |
| CN110697912A (en) * | 2019-11-27 | 2020-01-17 | 上源环工生态环境科技(苏州)有限公司 | Rapid and long-acting water treatment agent and preparation method thereof |
-
2004
- 2004-06-29 CN CN 200410019818 patent/CN1594551A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100374547C (en) * | 2005-12-28 | 2008-03-12 | 农业部环境保护科研监测所 | Composite bacillus subtilis and lactic acid bacteria microbe formulation preparation method |
| CN100441679C (en) * | 2006-11-24 | 2008-12-10 | 西安利能科技有限责任公司 | Bacterium composite nutrient |
| CN102583776A (en) * | 2012-02-29 | 2012-07-18 | 中国水产科学研究院淡水渔业研究中心 | Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method |
| CN102583776B (en) * | 2012-02-29 | 2013-04-24 | 中国水产科学研究院淡水渔业研究中心 | Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method |
| CN102583907A (en) * | 2012-03-21 | 2012-07-18 | 湖南富利来环保科技工程有限公司 | Organic sewage treatment system |
| CN110697912A (en) * | 2019-11-27 | 2020-01-17 | 上源环工生态环境科技(苏州)有限公司 | Rapid and long-acting water treatment agent and preparation method thereof |
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