CN1831112A - Prepn. used for repairing water ecological environment and its prepn. method - Google Patents
Prepn. used for repairing water ecological environment and its prepn. method Download PDFInfo
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- CN1831112A CN1831112A CN 200610013362 CN200610013362A CN1831112A CN 1831112 A CN1831112 A CN 1831112A CN 200610013362 CN200610013362 CN 200610013362 CN 200610013362 A CN200610013362 A CN 200610013362A CN 1831112 A CN1831112 A CN 1831112A
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- subtilis
- streptomyces microflavus
- fermentation lactobacillus
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- lactobacillus
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Abstract
The invention relates to a preparation and manufacture method to mend water ecological environment that is the optimized compound of wilted hay bac 60-80%, fine yellow streptomycete 10-20%, rancid lactobacillus 10-20%. The benefit effect of the invention is that it could have good mending effect for water ecological environment.
Description
Technical field
The present invention relates to a kind of preparation of repairing water ecological environment and preparation method thereof.
Background technology
At present the urban landscape water of China and culture fishery water, particularly raising fish and shrimp already develop and are faced with water pollution, and aquatic ecosystem is unbalance to cause prawn disease to spread, body eutrophication, and situation is serious.Along with the raising and the plan of national development pollution-free food of people's living standard, carry out the prevention and cure of shrimp disease current requirement of incompatibility to heath food with antibiotic medicine.
Fresh water and sea farming great majority adopt the mode of " sealing semiclosed internal recycle cultures " (the hydrostatic sealing is cultured) at present, promptly do not change water culturing in season, therefore cause bait and the excremental accumulation of aquaculture organism, summer, sweltering heat made water quality deterioration in addition, fishes and shrimps sickness rate height.In order to reduce disease loss, have to use in a large number microbiotic and chemicals, therefore further polluted water again causes fish and shrimp diseases to increase, the formation vicious cycle.Traditional breeding way not only makes the raising fish and shrimp cost up, and has a strong impact on the quality of fishes and shrimps, and sewage emissions causes serious environmental to pollute simultaneously.All seeking to improve water surrounding both at home and abroad, the effective ways of prevention fishes and shrimps disease.
Summary of the invention
Technical problem to be solved by this invention is: the preparation and preparation method thereof that the reparation water ecological environment of a kind of environmental protection, high density and activity is provided.
The present invention solves this technical problem the technical scheme that is adopted:
With subtilis [ACCC10619, Bacillus subtilis (Ehrenberg) Cohn, ] and fermentation lactobacillus [AS1.1880, Lactobacillus bulgaricus (Luerssen et kuhu) Holland] be main, be aided with an amount of streptomyces microflavus [AS4.891, (Streptomyces microflavus)], and adsorption of immobilization is prepared into bacteria preparation on wheat bran.
The micro-ecological bacterial preparation of repairing water surrounding is the optimization mixture of above-mentioned subtilis, streptomyces microflavus, fermentation lactobacillus composition, wherein the weight percent of each component is: subtilis 60～80%, streptomyces microflavus 10～20%, fermentation lactobacillus 10～20%.
The preparation method who repairs the preparation of water ecological environment comprises the steps:
1. each slant strains activation, shake-flask culture respectively of preserving.Subtilis and streptomyces microflavus Zulkovsky starch culture medium culturing, pH7.0～7.5,115～121 ℃ autoclaving 20-30 minute; Fermentation lactobacillus is directly cultivated with the sterilization fresh cow milk; Subtilis was cultivated 20～24 hours for 35～40 ℃; Streptomyces microflavus and fermentation lactobacillus were cultivated 36～50 hours for 28～30 ℃.
2. subtilis, the level liquid enlarged culturing of streptomyces microflavus and fermentation lactobacillus; Inoculum size 10%～20%.
3. subtilis, the secondary fluid enlargement culture of streptomyces microflavus and fermentation lactobacillus; Inoculum size 10%～20%.
4. with wheat bran carrier solid enlarged culturing and being immobilized onto on the wheat bran respectively.
5. with subtilis, streptomyces microflavus, 30～50 ℃ of warm air dryings of fermentation lactobacillus, pulverize then.
6. subtilis, streptomyces microflavus, fermentation lactobacillus are mixed, the weight percent of each bacterium component is: subtilis 60～80%, streptomyces microflavus 10～20%, fermentation lactobacillus 10～20%.
Beneficial effect of the present invention
1) composite bacteria is immobilized onto on the wheat bran, make effective bacterium colony number (CFU) of every gram composite bacteria preparation can be up to 1,000 hundred million, almost reached most probable number MPN, so far yet there are no the report of high bacteria containing amount bacteria preparation like this. wheat bran can not only provide some nutritive ingredients by being cultivated bacterium, also because its reticulated structure, has bigger specific surface area, so can in unit volume, adhere to the more thalline of adsorption of immobilization.
2) free NH
3And NO
2 -Being the main objectionable constituent of poisoning fishes and shrimps, also is to cause the shrimp main causes of death. if can reduce ammonia nitrogen (NH
4 +-N), free NH
3Also will reduce NO
2 --N also can reduce, and the pH value also just tends towards stability. and under the same dosage of similarity condition, we compare with the Bio-culture Aq (Bio-t) of U.S. Brookside company by the product (XIJ) of development, reduce NO
2 -The effect of-N amount is more or less the same, but reduces NH
4 +The ability of-N but obviously is better than Bio-t.Being spilled into water body after bacteria preparation is activated can the little ecological dominance bacterium of very fast formation, can obviously reduce the COD and the nitrite nitrogen of culturing water, can stablize ammonia-state nitrogen (NH
4 +-N) and remain on the certain level. make the pH value stabilization within the specific limits.
Positively effect of the present invention also is:
1. bacteria preparation of the present invention is activated, can the little ecological dominance flora of very fast formation in water, and the effect of bring into play its degradation of organic substances, shortly give birth to, benefit being given birth to;
2. can reduce COD rapidly;
3. reduction nitrite nitrogen;
4. ammonia-state nitrogen and pH value are remained on the certain level;
5. suppress the breeding of harmful germ, regulate algae density in the water, promote aquatic ecosystem reparation and balance;
6. promote shrimp gi tract health, compared with the control, the control cost reduces by 80%, and shrimp output improves nearly 1 times, and significantly improves the quality of shrimp;
7. replace or minimizing use microbiotic and chemicals, make shrimp farming become the nuisanceless green aquaculture;
8. it is strong that micro-ecological bacterial preparation of the present invention has adaptability, good to the water ecological environment repairing effect, characteristics such as viable count height;
9. can be applied to urban look water or regeneration water purification.
Embodiment
Embodiment 1
Follow these steps to carry out:
1) each slant strains with 4 ℃ of preservations activates, respectively shake-flask culture.Subtilis and streptomyces microflavus solubility
Starch culture-medium is cultivated, and it is as follows specifically to fill a prescription: Zulkovsky starch 20g, KNO
31g, NaCl 0.5g, K
2HPO
40.5g, MgSO
4.7H
2O 0.5g, FeSO
40.01g, distilled water 1000ml, pH7.2,121 ℃ of autoclavings 20 minutes.Fermentation lactobacillus is directly cultivated with the sterilization fresh cow milk.Subtilis was cultivated 24 hours for 37 ℃; Streptomyces microflavus and fermentation lactobacillus were cultivated 48 hours for 30 ℃.
2) level liquid enlarged culturing (substratum and culture condition are the same for subtilis, streptomyces microflavus and the independent enlarged culturing of fermentation lactobacillus difference); Inoculum size 10%.
3) secondary liquid difference enlarged culturing; Inoculum size 10%.
4) with wheat bran be carrier solid enlarged culturing and being immobilized onto on the wheat bran respectively.
5) with 35 ℃ of warm air dryings of above-mentioned three class bacterium, to pulverize then, the weight percent of pressing each bacterium component mixes: subtilis 600 grams, streptomyces microflavus 100 grams, fermentation lactobacillus 100 gram mixing can obtain preparation of the present invention.
Embodiment 2
The performance measurement of micro-ecological bacterial preparation
Selected 5 do not have the native pond shrimp-cultivation pool of inlet gate to experimentize.1～No. 4 pond is an experiment pool, and No. 5 the pond is the contrast pond.The nearly 2000m of each pond area
2, average depth 1.8m disposes one in same model oxygenating machine respectively.Experimental session does not replenish fresh water, average 17～18 ℃ of shrimp pool water temperature, salinity 2.0%～2.1%.The amount that adds composite bacteria preparation: add dosage: 18000g/hm for the first time
2, i.e. 1mg.L
-1, to add weekly once later on, dosage is 4500g/hm
2. bacteria preparation is to soak in 20 times of water, stir 1～1.5h before use earlier, behind the activation thalline, splashes in the pond equably again.The contrast pond does not add composite bacteria preparation.Experiment periods 3 months.
Measurement result: CODCr reaches 84.7%, (potassium dichromate process) with respect to contrast pond clearance.
Ammonia nitrogen: experiment pool increases afterwards earlier and reduces, and is stabilized in 4mg.L after three days
-1Below (nessler reagent method).The contrast pond, ammonia nitrogen is a trend of rising gradually, one week the back ammonia nitrogen reach 6～8mg.L
-1
Nitrite nitrogen: contrast pond nitrite nitrogen raises fast and maintains high level, reaches 10mg.L in the time of the 10th day
-1, experiment pool begins also slightly to raise, but maintain always very low-level, in the time of the 10th day only at 2mg.L
-1Below, the clearance of relative comparison pond nitrite nitrogen reaches more than 80%.
Cost keeping: it is 5400kg.hm that shrimp output is obtained in the contrast pond
2, prawn disease is controlled in input, the anti-disease medicines expense reaches 2500～3000 yuan.And the average 10500kg.hm of shrimp output of experiment pool
2, unit output increases nearly 1 times.Experiment pool is owing to only thrown in bacteria preparation, and every pond is roughly equal to 500 yuan, does not add any medicine again, so cost reduces greatly, is 1/5～1/6 of contrast pond approximately.
Claims (2)
1. microbial preparation of repairing water ecological environment, it is characterized in that: the optimization mixture that this bacteria preparation mainly is made up of subtilis, streptomyces microflavus, fermentation lactobacillus, wherein the weight percent of each bacterium component is: subtilis 60～80%, streptomyces microflavus 10～20%, fermentation lactobacillus 10～20%.
2. the preparation method of the preparation of reparation water ecological environment as claimed in claim 1 is characterized in that it comprises the steps:
1) each slant strains activation, the shake-flask culture respectively that will preserve; Subtilis and streptomyces microflavus Zulkovsky starch culture medium culturing, pH7.0～7.5,115～121 ℃ autoclaving 20～30 minutes; Fermentation lactobacillus is directly cultivated with the sterilization fresh cow milk; Subtilis was cultivated 20～24 hours for 35～40 ℃; Streptomyces microflavus and fermentation lactobacillus were cultivated 36～50 hours for 28～30 ℃;
2) subtilis, the level liquid enlarged culturing of streptomyces microflavus and fermentation lactobacillus (20L jar); Inoculum size 10%～20%;
3) subtilis, the secondary fluid enlargement culture of streptomyces microflavus and fermentation lactobacillus (200L jar); Inoculum size 10%～20%;
4) be that carrier carries out the solid enlarged culturing respectively and is immobilized onto on the wheat bran with wheat bran;
5), pulverize then with subtilis, streptomyces microflavus, 30～50 ℃ of warm air dryings of fermentation lactobacillus;
6) subtilis, streptomyces microflavus, fermentation lactobacillus are mixed, the weight percent of each bacterium component is: subtilis 60～80%, streptomyces microflavus 10～20%, fermentation lactobacillus 10～20%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610013362 CN1831112A (en) | 2006-03-24 | 2006-03-24 | Prepn. used for repairing water ecological environment and its prepn. method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610013362 CN1831112A (en) | 2006-03-24 | 2006-03-24 | Prepn. used for repairing water ecological environment and its prepn. method |
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| Publication Number | Publication Date |
|---|---|
| CN1831112A true CN1831112A (en) | 2006-09-13 |
Family
ID=36993611
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610013362 Pending CN1831112A (en) | 2006-03-24 | 2006-03-24 | Prepn. used for repairing water ecological environment and its prepn. method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361272A (en) * | 2012-04-05 | 2013-10-23 | 上海市农业科学院 | Ecological environmental restoration agent used for animal housing |
| CN105836900A (en) * | 2016-06-03 | 2016-08-10 | 北京荣蒂盛环境科技有限公司 | Ecological water purifying system |
| CN107916242A (en) * | 2017-12-29 | 2018-04-17 | 南昌工程学院 | A kind of peasant household's sewage disposal composite bacteria agent and preparation method thereof |
-
2006
- 2006-03-24 CN CN 200610013362 patent/CN1831112A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361272A (en) * | 2012-04-05 | 2013-10-23 | 上海市农业科学院 | Ecological environmental restoration agent used for animal housing |
| CN105836900A (en) * | 2016-06-03 | 2016-08-10 | 北京荣蒂盛环境科技有限公司 | Ecological water purifying system |
| CN107916242A (en) * | 2017-12-29 | 2018-04-17 | 南昌工程学院 | A kind of peasant household's sewage disposal composite bacteria agent and preparation method thereof |
| CN107916242B (en) * | 2017-12-29 | 2020-09-29 | 南昌工程学院 | Composite microbial inoculum for treating farmer sewage and preparation method thereof |
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