CN103361272A - Ecological environmental restoration agent used for animal housing - Google Patents
Ecological environmental restoration agent used for animal housing Download PDFInfo
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- CN103361272A CN103361272A CN2012100977084A CN201210097708A CN103361272A CN 103361272 A CN103361272 A CN 103361272A CN 2012100977084 A CN2012100977084 A CN 2012100977084A CN 201210097708 A CN201210097708 A CN 201210097708A CN 103361272 A CN103361272 A CN 103361272A
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- ecotope
- renovation agent
- nutrient solution
- sodium
- poultry house
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Abstract
The invention relates to an ecological environmental restoration agent used for an animal housing. The ecological environmental restoration agent is formed by compounding lactobacillus, bacillus and photosynthetic bacteria according to the ratio of viable count as (4-5): (3-4): 1; and the restoration agent contains more than 108CFU (colony-forming unit)/mL of beneficial microorganism based on the viable count. Compared with the prior art, the ecological environmental restoration agent is the complementation of an existing chemical disinfecting mode and has the advantages that the content of ammonia gas in the air in the animal housing can be reduced by 30% after the ecological environmental restoration agent is sprayed at fixed period during cultivating; and the harmful microbe can be effectively prevented from invading, so that the morbidity can be reduced; in addition, ecological bacteria adopted in the ecological environment restoration agent used for the animal housing is the usable ecological bacteria meeting the national provisions; and the ecological environmental restoration agent is safe to operate and practical.
Description
Technical field
The present invention relates to a kind of environment remediation agent, especially relate to a kind of poultry house ecotope renovation agent.
Background technology
The regular sterilization of poultry house is to ensure livestock and poultry cultivation Biosafety Main Means, and sterilizing agent commonly used is chemostefilant at present.But chemostefilant has also been killed beneficial microorganism in the poultry house in the kill harmful microorganism, and the microecological balance in the house is destroyed, and after spraying chemical disinfection liquid, poultry house is in sterile state, and the outer microorganism of house is easy to invasion.In addition, as using the conventional chemical sterilizing agent to carry out full animal house space disinfection, easily epidermis, eyes and the respiratory system of people, animal are produced chemical burns in the breeding process.
Summary of the invention
Purpose of the present invention is exactly to provide a kind of operational safety, practicality for the defective that overcomes above-mentioned prior art existence, can make the content of animal house Ammonia in Air gas reduce by 30% poultry house ecotope renovation agent.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of poultry house ecotope renovation agent, by lactobacillus, genus bacillus, photosynthetic bacterium in viable count ratio 4～5: 3～4: 1 composite formation, containing the beneficial microorganism viable count in the renovation agent is 10
8More than the CFU/mL.
Described lactobacillus adopts following methods to cultivate: behind the making lactic acid bacillus nutrient solution, press inoculum size inoculating lactobacillus in nutrient solution of 5v/v%, 37 ℃ leave standstill cultivation 48 hours.
Described lactobacillus nutrient solution is made of following component and content: the 0.5-2wt% peptone, the 0.2-3wt% extractum carnis, the 0.2-1wt% yeast extract, 0.2-2wt% glucose, the 0.2-2wt% sodium acetate, 0.1-0.5wt% citric acid diamines, 0.02-0.2wt% tween-80,0.1-0.5wt%K
2HPO
4, 0.001-0.01wt%MgSO
47H
2O is with H
2O is solvent.
The preferred following component of described lactobacillus nutrient solution and content: 1wt% peptone, 1wt% extractum carnis, 0.5wt% yeast extract, 0.5wt% glucose, 0.5wt% sodium acetate, 0.2wt% citric acid diamines, 0.1wt% tween-80,0.2wt%K
2HPO
4, 0.005wt%MgSO
47H
2O is with H
2O is solvent.
Described genus bacillus adopts following methods to cultivate: behind the preparation genus bacillus nutrient solution, inoculate genus bacillus by the inoculum size of 5v/v% in nutrient solution, 37 ℃ leave standstill cultivation 48 hours.
Described genus bacillus nutrient solution is made of following component and content: 0.2-1wt% glucose, and the 0.5-2wt% peptone, 0.1-1wt% sodium-chlor, the 0.2-0.8wt% extractum carnis is with H
2O is solvent.
The preferred following component of described genus bacillus nutrient solution and content: 0.5wt% glucose, the 1wt% peptone, 0.5wt% sodium-chlor, the 0.5wt% extractum carnis is with H
2O is solvent.
Described photosynthetic bacterium adopts following methods to cultivate: the preparation photosynthetic bacteria culture solution, in nutrient solution, inoculate photosynthetic bacterium by 1v/v%, and the control culture temperature is 25～35 ℃, illumination is 2000～3000lx, leaves standstill to cultivate 2～7 days.
Described photosynthetic bacteria culture solution is made of following component and content: sodium-acetate 1.1-1.2g/L, peptone 0.03-0.08g/L, sodium bicarbonate 0.4-1g/L, Sulfothiorine 0.2-1g/L, sodium-chlor 0.2-0.5g/L, sal epsom 0.05-0.2g/L, potassium primary phosphate 0.01-0.1g/L is with H
2O is solvent.
The preferred following component of described photosynthetic bacteria culture solution and content: sodium-acetate 1.145g/L, peptone 0.055g/L, sodium bicarbonate 0.6g/L, Sulfothiorine 0.4g/L, sodium-chlor 0.3g/L, sal epsom 0.1g/L, potassium primary phosphate 0.05g/L is with H
2O is solvent.
Compared with prior art, the present invention is replenishing of existing chemical disinfection mode; Periodically sprinkle in breeding process can make the content of animal house Ammonia in Air gas reduce 30%, can effectively prevent the harmful microorganism invasion simultaneously, reducing sickness rate; And poultry house ecotope renovation agent is employed to be the spendable ecological bacterium of national regulation, operational safety, practicality.
Description of drawings
Fig. 1 is that the poultry house ecotope safeguards that microbial inoculum is to the Dynamic Influence Diagrams of pig house Ammonia in Air gas content;
Fig. 2 is that poultry house ecotope renovation agent is to the Dynamic Influence Diagrams of pig house Carbon Dioxide in Air content.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
Poultry house ecotope renovation agent production method:
Difference pure culture lactobacillus, genus bacillus, photosynthetic bacterium 10L;
With the microbial inoculum that ferments respectively in viable count ratio 4～5: 3～4: 1 mixes, the viable count 10 of mixed bacteria liquid
8More than the CFU/mL.
Poultry house ecotope maintaniing agent using method:
With atomizer livestock and poultry ecotope renovation agent evenly is sprayed at slurry gutte in animal house air, ground, the house, spraying first dosage is 20g/m
3, spraying once every 10 days subsequently, sprinkling dosage is 3g/m
3
Poultry house ecotope maintaniing agent result of use: in order to verify its effect, two nursery houses are selected in this test on the pig farm, Shanghai City, use poultry house ecotope renovation agent in eight o'clock sharps of the morning.The pig house space is sprayed, before sprinkling, spray rear 30 minutes, spray 2 pm on the same day whole, and continuous 2 day subsequently 2 pm whole the airborne ammonia of animal house and carbonic acid gas are detected, every house selects sample at 3.Detected result is as follows:
Table 1 detects the poultry house ecotope and safeguards that microbial inoculum is on the impact of pig house Air quality
Fig. 1 is that the poultry house ecotope safeguards that microbial inoculum (has the same letter mark to represent each other there was no significant difference to the Dynamic Influence Diagrams of pig house Ammonia in Air gas content, represent each other significant difference without the same letter mark, p＜0.05), Fig. 2 is poultry house ecotope renovation agent (has the same letter mark to represent each other there was no significant difference to the Dynamic Influence Diagrams of pig house Carbon Dioxide in Air content, represent each other significant difference, p＜0.05 without the same letter mark).
Such as table 1 and Fig. 1,2 as can be known, sprayed behind the renovation agent 30 minutes, Ammonia in Air gas and gas concentration lwevel extremely significantly descend (p＜0.01), afternoon on the same day, Ammonia in Air gas and carbon dioxide content went up to some extent, but ammonia and gas concentration lwevel linearly descend in two days air subsequently, in the time of the 3rd day in the air in ammonia and gas concentration lwevel significantly be lower than spray before, sprayed the same day (p＜0.05), significantly be lower than the pig house (p＜0.05) that air cleaner is installed.The analysis of causes: when spraying renovation agent, bacterium liquid has direct absorption and neutralizing effect to ammonia and carbonic acid gas, so Ammonia in Air gas and gas concentration lwevel extremely significantly descend.But the quantity of useful microbe that sprays in the house on the same day is limited, to the Degradation of fecaluria a little less than, so Ammonia in Air gas and carbon dioxide content have the rise phenomenon in the afternoon 2 the time.But obviously improving in two days Air qualities subsequently, mainly is the breeding growth in animal house of beneficial microorganism in the renovation agent, and absorption and degraded obnoxious flavour ability are strengthened.According to this toward experimental observation, the Air quality in two weeks is better than the Air quality in 1 week after significantly being better than spraying after spraying, and the child care phase pig diarrhea rate significantly descend.
Embodiment 2
A kind of poultry house ecotope renovation agent, in 4: 3: 1 composite formation of viable count ratio, containing the beneficial microorganism viable count in the renovation agent is 10 by lactobacillus, genus bacillus, photosynthetic bacterium
8More than the CFU/mL.
Lactobacillus adopts following methods to cultivate: behind the making lactic acid bacillus nutrient solution, press inoculum size inoculating lactobacillus in nutrient solution of 5v/v%, 37 ℃ leave standstill cultivation 48 hours.The lactobacillus nutrient solution is made of following component and content: 0.5wt% peptone, 0.2wt% extractum carnis, 0.2wt% yeast extract, 0.2wt% glucose, 0.2wt% sodium acetate, 0.1wt% citric acid diamines, 0.02wt% tween-80,0.1wt%K
2HPO
4, 0.001wt%MgSO
47H
2O is with H
2O is solvent.
Genus bacillus adopts following methods to cultivate: behind the preparation genus bacillus nutrient solution, inoculate genus bacillus by the inoculum size of 5v/v% in nutrient solution, 37 ℃ leave standstill cultivation 48 hours.The genus bacillus nutrient solution is made of following component and content: 0.2wt% glucose, and the 0.5wt% peptone, 0.1wt% sodium-chlor, the 0.2wt% extractum carnis is with H
2O is solvent.
Photosynthetic bacterium adopts following methods to cultivate: the preparation photosynthetic bacteria culture solution, in nutrient solution, inoculate photosynthetic bacterium by 1v/v%, and the control culture temperature is 25 ℃, illumination is 3000lx, leaves standstill to cultivate 2 days.Photosynthetic bacteria culture solution is made of following component and content: sodium-acetate 1.1g/L, and peptone 0.03g/L, sodium bicarbonate 0.4g/L, Sulfothiorine 0.2g/L, sodium-chlor 0.2g/L, sal epsom 0.05g/L, potassium primary phosphate 0.01g/L is with H
2O is solvent.
Embodiment 3
A kind of poultry house ecotope renovation agent, in 4: 4: 1 composite formation of viable count ratio, containing the beneficial microorganism viable count in the renovation agent is 10 by lactobacillus, genus bacillus, photosynthetic bacterium
8More than the CFU/mL.
Lactobacillus adopts following methods to cultivate: behind the making lactic acid bacillus nutrient solution, press inoculum size inoculating lactobacillus in nutrient solution of 5v/v%, 37 ℃ leave standstill cultivation 48 hours.The lactobacillus nutrient solution comprises following component and content: 1wt% peptone, 1wt% extractum carnis, 0.5wt% yeast extract, 0.5wt% glucose, 0.5wt% sodium acetate, 0.2wt% citric acid diamines, 0.1wt% tween-80,0.2wt%K
2HPO
4, 0.005wt%MgSO
47H
2O is with H
2O is solvent.
Genus bacillus adopts following methods to cultivate: behind the preparation genus bacillus nutrient solution, inoculate genus bacillus by the inoculum size of 5v/v% in nutrient solution, 37 ℃ leave standstill cultivation 48 hours.The genus bacillus nutrient solution comprises following component and content: 0.5wt% glucose, and the 1wt% peptone, 0.5wt% sodium-chlor, the 0.5wt% extractum carnis is with H
2O is solvent.
Photosynthetic bacterium adopts following methods to cultivate: the preparation photosynthetic bacteria culture solution, in nutrient solution, inoculate photosynthetic bacterium by 1v/v%, and the control culture temperature is 30 ℃, illumination is 2500lx, leaves standstill to cultivate 5 days.Photosynthetic bacteria culture solution comprises following component and content: sodium-acetate 1.145g/L, and peptone 0.055g/L, sodium bicarbonate 0.6g/L, Sulfothiorine 0.4g/L, sodium-chlor 0.3g/L, sal epsom 0.1g/L, potassium primary phosphate 0.05g/L is with H
2O is solvent.
Embodiment 4
A kind of poultry house ecotope renovation agent, in 5: 4: 1 composite formation of viable count ratio, containing the beneficial microorganism viable count in the renovation agent is 10 by lactobacillus, genus bacillus, photosynthetic bacterium
8More than the CFU/mL.
Lactobacillus adopts following methods to cultivate: behind the making lactic acid bacillus nutrient solution, press inoculum size inoculating lactobacillus in nutrient solution of 5v/v%, 37 ℃ leave standstill cultivation 48 hours.The lactobacillus nutrient solution is made of following component and content: 2wt% peptone, 3wt% extractum carnis, 1wt% yeast extract, 2wt% glucose, 2wt% sodium acetate, 0.5wt% citric acid diamines, 0.2wt% tween-80,0.5wt%K
2HPO
4, 0.01wt%MgSO
47H
2O is with H
2O is solvent.
Genus bacillus adopts following methods to cultivate: behind the preparation genus bacillus nutrient solution, inoculate genus bacillus by the inoculum size of 5v/v% in nutrient solution, 37 ℃ leave standstill cultivation 48 hours.The genus bacillus nutrient solution is made of following component and content: 1wt% glucose, and the 2wt% peptone, 1wt% sodium-chlor, the 0.2-0.8wt% extractum carnis is with H
2O is solvent.
Photosynthetic bacterium adopts following methods to cultivate: the preparation photosynthetic bacteria culture solution, in nutrient solution, inoculate photosynthetic bacterium by 1v/v%, and the control culture temperature is 35 ℃, illumination is 2000lx, leaves standstill to cultivate 7 days.Photosynthetic bacteria culture solution is made of following component and content: sodium-acetate 1.2g/L, and peptone 0.08g/L, sodium bicarbonate 1g/L, Sulfothiorine 1g/L, sodium-chlor 0.5g/L, sal epsom 0.2g/L, potassium primary phosphate 0.1g/L is with H
2O is solvent.
Claims (10)
1. a poultry house ecotope renovation agent is characterized in that, this ecotope renovation agent by lactobacillus, genus bacillus, photosynthetic bacterium in viable count ratio 4～5: 3～4: 1 composite formation, containing the beneficial microorganism viable count in the renovation agent is 10
8More than the CFU/mL.
2. a kind of poultry house ecotope renovation agent according to claim 1, it is characterized in that, described lactobacillus adopts following methods to cultivate: behind the making lactic acid bacillus nutrient solution, press inoculum size inoculating lactobacillus in nutrient solution of 5v/v%, 37 ℃ leave standstill cultivation 48 hours.
3. a kind of poultry house ecotope renovation agent according to claim 2, it is characterized in that, described lactobacillus nutrient solution is made of following component and content: the 0.5-2wt% peptone, the 0.2-3wt% extractum carnis, 0.2-1wt% yeast extract, 0.2-2wt% glucose, the 0.2-2wt% sodium acetate, 0.1-0.5wt% citric acid diamines, 0.02-0.2wt% tween-80,0.1-0.5wt%K
2HPO
4, 0.001-0.01wt%MgSO
47H
2O is with H
2O is solvent.
4. a kind of poultry house ecotope renovation agent according to claim 3, it is characterized in that, the preferred following component of described lactobacillus nutrient solution and content: 1wt% peptone, the 1wt% extractum carnis, 0.5wt% yeast extract, 0.5wt% glucose, the 0.5wt% sodium acetate, 0.2wt% citric acid diamines, 0.1wt% tween-80,0.2wt%K
2HPO
4, 0.005wt%MgSO
47H
2O is with H
2O is solvent.
5. a kind of poultry house ecotope renovation agent according to claim 1, it is characterized in that, described genus bacillus adopts following methods to cultivate: behind the preparation genus bacillus nutrient solution, inoculate genus bacillus by the inoculum size of 5v/v% in nutrient solution, 37 ℃ leave standstill cultivation 48 hours.
6. a kind of poultry house ecotope renovation agent according to claim 5 is characterized in that, described genus bacillus nutrient solution is made of following component and content: 0.2-1wt% glucose, the 0.5-2wt% peptone, 0.1-1wt% sodium-chlor, the 0.2-0.8wt% extractum carnis is with H
2O is solvent.
7. a kind of poultry house ecotope renovation agent according to claim 6 is characterized in that, the preferred following component of described genus bacillus nutrient solution and content: 0.5wt% glucose, and the 1wt% peptone, 0.5wt% sodium-chlor, the 0.5wt% extractum carnis is with H
2O is solvent.
8. a kind of poultry house ecotope renovation agent according to claim 1, it is characterized in that, described photosynthetic bacterium adopts following methods to cultivate: the preparation photosynthetic bacteria culture solution, in nutrient solution, inoculate photosynthetic bacterium by 1v/v%, the control culture temperature is 25～35 ℃, illumination is 2000～3000lx, leaves standstill to cultivate 2～7 days.
9. a kind of poultry house ecotope renovation agent according to claim 8, it is characterized in that, described photosynthetic bacteria culture solution is made of following component and content: sodium-acetate 1.1-1.2g/L, peptone 0.03-0.08g/L, sodium bicarbonate 0.4-1g/L, Sulfothiorine 0.2-1g/L, sodium-chlor 0.2-0.5g/L, sal epsom 0.05-0.2g/L, potassium primary phosphate 0.01-0.1g/L is with H
2O is solvent.
10. a kind of poultry house ecotope renovation agent according to claim 9, it is characterized in that, the preferred following component of described photosynthetic bacteria culture solution and content: sodium-acetate 1.145g/L, peptone 0.055g/L, sodium bicarbonate 0.6g/L, Sulfothiorine 0.4g/L, sodium-chlor 0.3g/L, sal epsom 0.1g/L, potassium primary phosphate 0.05g/L is with H
2O is solvent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100977084A CN103361272A (en) | 2012-04-05 | 2012-04-05 | Ecological environmental restoration agent used for animal housing |
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|---|---|---|---|
| CN2012100977084A CN103361272A (en) | 2012-04-05 | 2012-04-05 | Ecological environmental restoration agent used for animal housing |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823148A (en) * | 2016-03-30 | 2016-08-03 | 宁波艾克威特智能科技有限公司 | Heavy metal resisting type filtering device used for air cleaner, and installing and using method thereof |
| CN107691477A (en) * | 2017-09-16 | 2018-02-16 | 安徽华澳生物技术有限公司 | A kind of poultry disinfectant |
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Application publication date: 20131023 |