CN103952360A - Cleaning agent for environment in livestock house and application thereof - Google Patents
Cleaning agent for environment in livestock house and application thereof Download PDFInfo
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- CN103952360A CN103952360A CN201410193036.6A CN201410193036A CN103952360A CN 103952360 A CN103952360 A CN 103952360A CN 201410193036 A CN201410193036 A CN 201410193036A CN 103952360 A CN103952360 A CN 103952360A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention relates to a cleaning agent for environment in a livestock house and an application thereof. The cleaning agent comprises bacillus thuringiensis and bacillus amyloliquefaciens. The bacillus thuringiensis and the bacillus amyloliquefaciens are dissolved in warm water according to a proportion of 1:(1-3), and then the solution is uniformly sprayed in the livestock house, so that per cubic meter of air contains 1*10<8>CFU/m<3>-9*10<9>CFU/m<3> of bacillus thuringiensis and 1*10<8>CFU/m<3>-3*10<10>CFU/m<3> of bacillus amyloliquefaciens. The cleaning agent can purify the air in the livestock house, lower the content of ammonia gas in the livestock house by 9%-33% and the content of hydrogen sulfide by 45%-76%, kill pathogenic microorganisms in the air, and lower staphylococcus in the air by 29% and escherichia coli in the air by 25%, thus achieving the purpose of purifying the environment in the livestock house. Compared with the prior art, the cleaning agent has the advantages of no pollution, no residue, no drug resistance, safety in use, simple operation and the like, is environment-friendly to the environment in the livestock house, effectively improves the air quality and increases the livestock breeding benefits.
Description
Technical field
The invention belongs to livestock and poultry cultivation technical field, relate to probiotics and application method, be specifically related to environmental purifying agent and application method thereof in a kind of poultry house.
Technical background
In current intensive culture pattern, livestock and poultry are mainly concentrated and raise in airtight poultry house.Because cultivation density is high, in poultry house, environment very easily worsens, and has a strong impact on growth and the health of livestock and poultry.In house, environmental degradation main manifestations is that obnoxious flavour is as the concentration rising of the gases such as ammonia, hydrogen sulfide, carbonic acid gas.Excessive obnoxious flavour, in respiratory tract enters livestock and poultry body, can cause the anesthesia (Frank et al., 1983) of central nervous system.When in pig house, the content of ammonia reaches 50ppm, its weightening finish can decline 12%, and when the content of ammonia reaches 100ppm, the weightening finish of pig declines up to 30% (easy China etc., 1999).The too high existing anaemia in livestock and poultry mountain and the histanoxia of also can causing of ammonia concentration, reduces body's immunity, poor growth (An Lirong etc., 2010).
Ventilation can effectively improve environmental quality in poultry house, but winter ventilation can cause temperature in poultry house to reduce, and for keeping temperature in house, generally adopts minimum ventilation winter, often causes environmental degradation in poultry house.Improving at present the conventional method of environment in poultry house is to spray chemostefilant, comprise quaternary ammonium salts, formaldehyde, potassium permanganate, Iodophor, Peracetic Acid etc., these chemostefilants are also killed some beneficial microorganisms in kill harmful microorganism, cause Dysbiosis in environment, also easily cause the generation of bacterial drug resistance, and livestock and poultry body surface is produced to chemical damage simultaneously.In addition, can also in feed, add zymin or microbial preparation etc. and improve environment.As utilize proteolytic enzyme, amylase, citric acid etc. to be mixed with fodder additives, to reduce harmful gas in livestock house (publication CN102580516A), adopt subtilis, aspergillus oryzae etc. to be combined into fodder additives, in order to reduce poultry house ammonia concentration (publication CN102178059A).Directly in environment, spraying probiotics is also a kind of effective means.As utilize lactobacillus, genus bacillus and photosynthetic bacterium to be re-dubbed liquid spray agent, can reduce animal house ammonia concentration (publication CN103361272A).Genus bacillus is generic name, comprises altogether 22 kinds, and as Bacillus licheniformis, subtilis, bacillus megaterium, bacillus cereus etc., every kind of genus bacillus has different biological characteristicses.Research is found, is not that every kind of genus bacillus all has the harmful microorganism of inhibition propagation, reduces the effect that obnoxious flavour produces.And wherein bacillus thuringiensis is in forming the process of gemma, endobacillary one or both ends can form parasporal crystal, and insect is had to toxicity, as microbial pesticide, are widely used in plant protection.Bacillus amyloliquefaciens is secreted in growth metabolism process can Antifungi and Broad spectrum antibiotics, antibacterial protein or polypeptide class, the fat peptide matters of pathogenic bacteria, aspect biological control, is widely used.The present invention utilizes the effect of Bacillus thuringiensis Against worm, after poultry house environment sprays, can effectively reduce growing of mosquitos and flies; Utilize bacillus amyloliquefaciens broad-spectrum antimicrobial characteristic, and metabolism produces diastatic characteristic, after spraying, can effectively suppress the pathogenic micro-organism in environment in poultry house environment, reduce the obnoxious flavour that pathogenic micro-organism metabolism produces.
Summary of the invention
The object of this invention is to provide a kind of preparation of purifying agent and application method that improves environment in poultry house.Specifically,
Bacillus thuringiensis and bacillus amyloliquefaciens are dissolved in to warm water (being less than 37 degree) according to the ratio of 1:1-3, make suspension liquid, wherein the concentration of bacillus thuringiensis should not be lower than 1 * 10
7cFU/ml, the concentration of bacillus amyloliquefaciens should not be lower than 1 * 10
7cFU/ml.
After suspension liquid is fully shaken up, use atomizer to be evenly sprayed in the interior environment of poultry house, make every cubic metre of space in house contain bacillus thuringiensis 1 * 10
8cFU/m
3-9 * 10
9cFU/m
3, bacillus amyloliquefaciens 1 * 10
8cFU/m
3-3 * 10
10cFU/m
3.According to the age in days of livestock and poultry, within every 1-3 days in house, spray 1-2 time.
Evidence of the present invention, spray the present invention and carry after the scavenging agent on mountain, in house, Ammonia in Air gas content can reduce 9.3-33.8%, and hydrogen sulfide content can reduce 45.8-76.8%, in air, staphylococcus quantity can reduce 29.2-43.7%, and intestinal bacteria quantity can reduce 25.8-79.9%.
Bacillus thuringiensis of the present invention refers to a kind of into bacillus, and its vegetative cell form is the shaft-like of the blunt circle in two ends, can be in cell one end or two ends form parasporal crystal albumen, insect is had to killing action.Bacillus amyloliquefaciens is a kind of of bacillus, its vegetative cell form is shaft-like, can form endogenous spore, ovalize, the blunt circle in two ends, sporangium is not expanded, middle life is raw to time end, have mobility, can produce amylase and proteolytic enzyme, can produce a series of can Antifungi and the metabolite of bacterial activity.
The bacterial classification of bacillus thuringiensis of the present invention and bacillus amyloliquefaciens can be bought from national DSMZ, or separated from environment, also process bacterial classification preservation center evaluation, then expands according to conventional methods numerous forming.Also can directly buy from the market finished product, but need to identify through DSMZ.
Embodiment
The experimental technique using in following embodiment if no special instructions, is ordinary method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
1, the activation of bacterial strain and enlarged culturing
From national DSMZ, buy bacillus thuringiensis bacterial classification (1.1754) and bacillus amyloliquefaciens bacterial classification (1.769), through activation, seed liquor shake-flask culture, fermentor cultivation, the drying and other steps of bacterial classification, can obtain respectively the dry powder formulations of two kinds of bacterium.
Actication of culture: after slant tube (LB solid medium) inoculation, be placed in biochemical cultivation case, 30 ℃ of incubator 24h.
Seed liquor shake-flask culture: get 121 ℃ of one-tenth sterilized water 3ml after autoclaving 30min, the genus bacillus on wash-out slant medium, makes bacteria suspension.Get respectively 1mL genus bacillus bacteria suspension, be inoculated in the 250mL triangular flask that fills 100mL seed culture medium (LB liquid nutrient medium).Put 30 ℃ of shaking culture 24h in shaking table, rotating speed is 120r/min.That acquisition flushes, quantity sufficient seed nutrient solution.
Fermentor cultivation: genus bacillus seed liquor is inoculated in fermentor tank by 5% inoculum size, and fermentation condition is 30 ℃, 200r/min, initial pH7.0, in fermenting process, pH, oxyty (DO value) are carried out on-line monitoring.Fermention medium is extractum carnis 0.3%, peptone 0.5%, glucose 1.0%, NaCl0.5%, pH7.0-7.2.36-48h continuously ferments.
Dry: the liquid fermentation liquid that fermentation is completed is dry in freeze drier, makes dry powder.Bacillus thuringiensis and bacillus amyloliquefaciens dry powder are pulverized to rear 60 mesh sieves of crossing, utilize colony counting method to measure the viable count in dry powder.Every gram, bacillus thuringiensis dry powder that this laboratory ferment is produced is containing 4 * 10
10cFU, every gram, bacillus amyloliquefaciens dry powder is containing 2 * 10
10cFU.
2, the preparation of scavenging agent
Take respectively 7.5g bacillus thuringiensis and 20g bacillus amyloliquefaciens, after mixing, add 1000ml warm water (being less than 37 degree),
Make the concentration of bacillus thuringiensis reach 6 * 10
8cFU/ml, the concentration of bacillus amyloliquefaciens reaches 4 * 10
8cFU/ml, pours in miniaturised nebuliser after stirring.The suspension liquid being mixed with need be used within 12h, before use, shakes up.
3, the use of scavenging agent and effect
Choose 2 commercial broiler flocks houses (cultivation scale is 5000 /) that envrionment conditions is consistent, be divided at random test group and control group, every hen house is divided into 6 regions with net sheet, and each region is a test repeating unit.Test group hen house is used miniaturised nebuliser, and scavenging agent is evenly sprayed in poultry house in environment, and 2ml (should not surpass 10ml, otherwise can strengthen the humidity in house) is sprayed in every cubic metre of space, makes the interior every cubic metre of space of house contain bacillus thuringiensis 2 * 10
8cFU/m
3, bacillus amyloliquefaciens 3 * 10
8cFU/m
3.Control group sprays the distilled water of same dose.Before broiler chicken 21 ages in days, within every 2 days, spray 1 time, after 21 ages in days, spray 1 every day.At 14,21,28,35 ages in days, measured Air quality the same day respectively.Utilize ammonia detector and H2S detector to measure ammonia, the concentration of hydrogen sulfide in broiler house, utilize air bells laid method to measure staphylococcus and colibacillary quantity in air.Mean number ± standard deviation for measurement result (SD) represents, adopts the significance of difference between two treatment group of T check analysis, and significance of difference level is P<0.05.
The impact of environmental purifying agent on broiler house ammonia, concentration of hydrogen sulfide
By upper table, can see mountain, along with the increase of broiler chicken age in days, in hen house, the concentration of ammonia and hydrogen sulfide raises gradually, and this may increase rear movement generation with broiler chicken age in days and strengthen relevant.Spray after scavenging agent the interior NH of hen house in each week age
3all significantly reduce (P<0.05) with the concentration of hydrogen sulfide.Wherein ammonia concentration has reduced 9.3-33.8%, and hydrogen sulfide content has reduced 45.8-76.8%, shows that the action effect of scavenging agent is remarkable.
The impact of environmental purifying agent on broiler house air microbe content
As can be seen from the above table, along with the increase of broiler chicken age in days, in chicken coop air, staphylococcus and colibacillary concentration raise gradually, and this may be that activity increases because broiler chicken age in days increases, due to the aerosol that causes microbes in air and dust to form increases.Spray after scavenging agent staphylococcus and all significantly reductions (P<0.05) of colibacillary concentration in each week age chicken coop air.Wherein in air, staphylococcus quantity has reduced 29.2-43.7%, and intestinal bacteria quantity has reduced 25.8-79.9%, shows that the action effect of scavenging agent is remarkable.
Above embodiment is the detailed description to environmental purifying agent and application thereof in poultry house of the present invention, illustrative and nonrestrictive, for a person skilled in the art, can be according to modifying in institute's limited range, or part technical characterictic is wherein equal to replacement; Therefore in the variation and the modification that do not depart under general plotting of the present invention, within should belonging to protection scope of the present invention.
Claims (3)
1. environmental purifying agent and an application thereof in poultry house, is characterized in that:
By bacillus thuringiensis and bacillus amyloliquefaciens according to 1: the ratio of 1-3 is dissolved in warm water (being less than 37 degree), makes suspension liquid, after fully shaking up, uses atomizer to be evenly sprayed in poultry house in environment, and every cubic metre of space in house is contained:
Bacillus thuringiensis: 1 * 10
8cFU/m
3-9 * 10
9cFU/m
3;
Bacillus amyloliquefaciens: 1 * 10
8cFU/m
3-3 * 10
10cFU/m
3;
According to livestock and poultry age in days, take within every 1-3 days, to spray 1-2 time, can effectively reduce in poultry house ammonia level and hydrogen sulfide content in environment, reduce harmful microorganism quantity in the interior environment of poultry house, there is the features such as pollution-free, noresidue simultaneously.
2. a kind of poultry house environmental purifying agent according to claim 1 and application thereof, it is characterized in that, described bacillus thuringiensis is a kind of of bacillus, the form of its vegetative cell is the shaft-like of the blunt circle in two ends, can be in cell one end or two ends form parasporal crystal albumen, insect is had to killing action.
3. a kind of poultry house environmental purifying agent according to claim 1 and application thereof, it is characterized in that, what described bacillus amyloliquefaciens was bacillus is a kind of, and its vegetative cell form is shaft-like, can form endogenous spore, ovalize, the blunt circle in two ends, sporangium is not expanded, middle life is raw to time end, have mobility, can produce amylase and proteolytic enzyme, can produce a series of can Antifungi and the metabolite of bacterial activity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410193036.6A CN103952360A (en) | 2014-05-09 | 2014-05-09 | Cleaning agent for environment in livestock house and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410193036.6A CN103952360A (en) | 2014-05-09 | 2014-05-09 | Cleaning agent for environment in livestock house and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103952360A true CN103952360A (en) | 2014-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410193036.6A Pending CN103952360A (en) | 2014-05-09 | 2014-05-09 | Cleaning agent for environment in livestock house and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105749645A (en) * | 2016-03-30 | 2016-07-13 | 宁波艾克威特智能科技有限公司 | Insect resistant type filter device for air purifier and installation and using method thereof |
| ITUB20160457A1 (en) * | 2016-02-02 | 2017-08-02 | Maurizio Cacicia | GAS PURIFICATION PLANT (AIR) |
| CN111849831A (en) * | 2020-08-05 | 2020-10-30 | 福建洛东生物技术有限公司 | Bacillus subtilis, preparation and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1032840C (en) * | 1993-02-19 | 1996-09-25 | 北京农业大学 | Production-increasing compounded bacterium and its production method |
| CN1274702A (en) * | 2000-06-14 | 2000-11-29 | 吉林大学 | Composite biological bacteria preparation and its preparation process and application in preparing bacterial manure |
-
2014
- 2014-05-09 CN CN201410193036.6A patent/CN103952360A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1032840C (en) * | 1993-02-19 | 1996-09-25 | 北京农业大学 | Production-increasing compounded bacterium and its production method |
| CN1274702A (en) * | 2000-06-14 | 2000-11-29 | 吉林大学 | Composite biological bacteria preparation and its preparation process and application in preparing bacterial manure |
Non-Patent Citations (1)
| Title |
|---|
| 仝永娟等: "三株芽孢杆菌抑菌活性分析及对肉鸡舍空气微生物的影响", 《中国农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITUB20160457A1 (en) * | 2016-02-02 | 2017-08-02 | Maurizio Cacicia | GAS PURIFICATION PLANT (AIR) |
| CN105749645A (en) * | 2016-03-30 | 2016-07-13 | 宁波艾克威特智能科技有限公司 | Insect resistant type filter device for air purifier and installation and using method thereof |
| CN105749645B (en) * | 2016-03-30 | 2018-09-14 | 刘晓冰 | A kind of pest-resistant type air purifier filter device and its installation and application method |
| CN111849831A (en) * | 2020-08-05 | 2020-10-30 | 福建洛东生物技术有限公司 | Bacillus subtilis, preparation and application thereof |
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Application publication date: 20140730 |