CN1274702A - Composite biological bacteria preparation and its preparation process and application in preparing bacterial manure - Google Patents
Composite biological bacteria preparation and its preparation process and application in preparing bacterial manure Download PDFInfo
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- CN1274702A CN1274702A CN 00106372 CN00106372A CN1274702A CN 1274702 A CN1274702 A CN 1274702A CN 00106372 CN00106372 CN 00106372 CN 00106372 A CN00106372 A CN 00106372A CN 1274702 A CN1274702 A CN 1274702A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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Abstract
Composite biological bacteria includes eight kinds of bacteria, i. e., cellulose decomposing bacteria, starch decomposing bacteria, Bacillus licheniformis, saccharomycetes, azotobacteria, P bacteria, K bacteria and bacillus thuringiensis. It is prepared through separate slant culture, the first culture, mixing and the second culture. It may be used widely in preparing bacteria manure with wide application soil range, obvious yield increasing effect and excellent antiviral and disease-resisting effect.
Description
The present invention relates to a kind of bacteria agent of preparing by bacterial manure and preparation method thereof that can be used for, particularly relate to and a kind ofly contain multiple bacterial classification and can be used for preparing the composite fungus agent of bio-bacterial manure and the preparation method of mixed culture thereof.
Abroad development and the application facet at bio-feritlizer starts to walk early, in China application arranged also as the vinelandii of the U.S., the BOM bacterium of Japan, the land of happiness bacterium of Korea S etc.But price is higher, function singleness, and liquid state is used inconvenience, and popularization is restricted.China should start to walk not evening on the one hand at this.Just there was large scale application in five sixties, and had played good effect of increasing production.But because many places do not satisfy the requirements, science and technology falls behind, and quality does not guarantee.Enter the latter stage eighties, bio-bacterial manure is risen in China again, (Sun Yi according to the literature, the effect of 1990 organic fertilizer in modern agriculture, natural science yearbook 2 31-39) mainly contain potassium bacterium, Pekinese's fertility height in yield increasing fungus, the Hebei of Peking University, god 901 of Ministry of Astronautics Industry etc., but wherein majority is a single culture, function singleness, the effect that has is not remarkable.
The object of the present invention is to provide a kind of contain multiple bacterial classification can be used for composite fungus agent for preparing bio-bacterial manure and preparation method thereof.
The composite fungus agent that being used for of the present invention relates to prepares bio-bacterial manure contains following bacterial classification (in the unit volume bacterium number than):
Cellulose-decomposing bacterium (10-30%), amylolysis bacterium (10-30%), Bacillus licheniformis (10-30%), yeast (10-30%), vinelandii (10-20%), P bacterium (10-30%), K bacterium (10-20%), Bacillus thuringiensis (10-30%).
Culture of strains:
1, inclined-plane seed culture: eight kinds of bacterium connect the inclined-plane respectively, cultivate 24-36 hour, respectively join nothing then
In the bacterium water (test tube), vibrated 30 minutes, treat that evenly the back makes every examination with the blood counting chamber counting
The bacterium number average of various bacterium is 10 in the pipe
8Individual/ml (as deficiency, then can strengthen the bacterium amount that connects on the one hand,
Incubation time on the other hand can extend), get bacteria suspension;
2, first order seed is cultivated: the bacteria suspension that eight kinds of bacterium are respectively got 1ml is put into eight 250ml triangular flasks respectively,
Put into nutrient solution in every triangular flask respectively, wherein P bacterium, K bacterium, amylolysis bacterium, Soviet Union
Cloud gold bacillus, Bacillus licheniformis be with No. 2 substratum (being made into nutrient solution with distilled water, same down),
Vinelandii are trained with No. 36 with No. 13 substratum, cellulose-decomposing bacterium with No. 3 substratum, yeast
Support base, shaking table (180 rev/mins) was cultivated 36-72 hour for 30 ℃, with the counting method of blood cell counting,
Bacterium is counted content and reaches 10 in every kind of microbial inoculum
8Individual/ml, pressing as above, bacterium number ratio mixes eight kinds of microbial inoculums;
3, secondary seed is cultivated: get mixed microbial inoculum and put into the 500ml triangular flask, connecing the bacterium amount is 10%,
Substratum is selected peptone 5-15 gram/100ml, NaCl 2-8 gram/100ml, extractum carnis 1-5 gram for use
/ 100ml, MgSO
47H
2O 5-15 gram/100ml, FeSO
47H
2O 0.01-0.05 gram/100ml,
K
2HPO
40.5-3 gram/100ml, Na
2HPO
40.1-1 gram/100ml, Xylo-Mucine 0.5-5
Gram/100ml, CaCl
20.05-0.5 gram/100ml, sucrose 2-8 gram/100ml, (NH
4)
2SO
40.5-3
Gram/100ml mixes, and all the other are distilled water, and shaking table (180 rev/mins of revolutions) is cultivated 36-72 for 30 ℃
Hour, the pH value is 7.0-7.5, the counting method of blood cell counting contrasts with colony counting method simultaneously,
Make the bacterium number reach 3-8 * 10
9Individual/ml, promptly get composite fungus agent;
The composite bacteria agent of the present invention's preparation adds auxiliary material and then can be made into bio-bacterial manure, below carries out detailed narration.
A, be applied to the preparation of the abundant bio-bacterial manure of soil with organic matter: composite fungus agent (5-10%), wilkinite (20-36%), zeolite powder (20-40%), vermiculite power (15-40%) are mixed, stir, sampling Detection (colony counting method) makes that to contain effective viable count in the bio-bacterial manure be hundred million of 2-3/gram, packing, envelope promptly get bio-bacterial manure.
The quality of microbial inoculum (colony counting method): the aperture is 2.5-4.5mm, and it is hundred million of 2-3 that every gram contains effective work (compound) bacterium number, assorted bacterium number≤10%, and water content 20-35%, pH value 6.0-7.2 all meets the agricultural industry criteria NY227-94 of the People's Republic of China (PRC).
The fertilizer efficiency experiment of composite biological fertilizer:
1, indoor wheat plantation experiment:
| Numbering | Big plant height (cm) | Da Ye wide (cm) | Stem girth (cm) | ||
| In earlier stage | Mid-term | In earlier stage | Mid-term | Mid-term | |
| 2g microbial inoculum/basin | ????9.07 | ????22.40 | ????0.30 | ????0.40 | ????0.53 |
| 3g microbial inoculum/basin | ????9.59 | ????27.07 | ????0.35 | ????0.42 | ????0.62 |
| 4g microbial inoculum/basin | ????9.63 | ????27.50 | ????0.36 | ????0.42 | ????0.63 |
| 5g castor agent/part | ????9.60 | ????27.08 | ????0.36 | ????0.41 | ????0.62 |
| Plain soil | ????10.20 | ????25.08 | ????0.43 | ????0.36 | ????·0.51 |
As can be seen from the table, the wheat growing way of making fertilizer with microbial inoculum is significantly better than plain soil.Along with the increase of bacterial manure consumption, the growing way situation of wheat improves.
2, field planting experiment:
This composite biological fertilizer has carried out the planting experiment of many kinds in each department, can promote crop growth significantly, strengthen abilities such as farm crop are drought-resistant, resistant to lodging, disease and insect resistance, guarantee in fertility abundance in each of crop growth in period, improve crop quality, overcome unfavorable factors such as the residual harmful thing increases in single soil compaction that causes to fertilize, soil fertility decline, the farming object simultaneously, thereby reached the increasing both production and income purpose.
Show through a large amount of field planting experiments: this composite biological fertilizer is used on corn, the highest amount of increase in production reaches 28.7%, input-output ratio is 1: 10-35, and make corn early emerge, dial joint, heading stage, ripening stage in advance, have drought resisting, excellent results such as resistant to lodging;
This bio-bacterial manure can make paddy rice average early emerge 1-2 days, tiller number increase 1-8.2, every square metre number of productive ear to be increased 7.4-64.9, plant height, spike length and increases to some extent, sterile grain rate descends, period of seedling establishment is done sth. in advance 1-4 days, full heading time early 3-7 days, and the ripening stage shifts to an earlier date 2-6 days; Amount of increase in production average out to 9.96%, input-output ratio are about 1: 10; Effect at the anti-pestalotiopsis funerea in the saline-alkali wetland district in Baicheng;
The planting experiment of winter wheat shows, can make that seedling is neat, seedling strong, increases tiller number, improves cold tolerance, and obvious to prevention gaeumannomyces graminis disease effect especially, preventive effect reaches more than 95%, improves output 10-15%;
Cucumber, potato class, celery, planting experiment show that effect of increasing production is all arranged, also show effects such as resistant to lodging, disease-resistant, drought resisting simultaneously.
In sum, as can be seen, this bio-bacterial manure has increasing both production and income, improves the soil, preventing disease and pest, aspect such as resistant to lodging have good effect to farm crop.
B: but also proof of experiment, above-mentioned bio-bacterial manure only just have obvious effects on the abundant soil of organic matter, and its reason is only under this condition, and the various bacterium in the bacterial manure just might breed in a large number, produce the nutrient of a large amount of various adaptation crop growths.For the bacterial manure that composite bacteria agent of the present invention is made can be applied even more extensively in different soil properties, the actual needs that suits large area to popularize and use, generally in bio-bacterial manure, also need increase organic auxiliary material, as people, poultry, excrement of animals, tangerine bar straw, contain organic abundant auxiliary materials such as refuse soil after treatment.
Document show (the Shanghai environmental science, producing fertilizer from refuse in daily life dynamic process research, old generation and etc.; 11 (5); 13-15,1992), in the domestic refuse that destructor plant adopts composting technology to handle, contain great number of organic matters; as abundant nitrogen, phosphorus, potassium are arranged in the domestic refuse; the Schwellenwert of being measured is: nitrogen content 2367mg/kg, quick-acting nitrogen 369mg/kg, total phosphorus 1652mg/kg; total potassium 24664mg/kg, available potassium reaches 9783mg/kg.Illustrate and containing huge energy in the domestic refuse that the someone claims that domestic refuse is that " reproducible second mineral reserve " are confirmed.
Above-mentioned composite fungus agent is sprayed onto on the rubbish of handling well, makes that the composite bacteria number reaches hundred million/g of 2-3 in the refuse soil, then make the efficient biologic-organic fertilizer that contains many bacterial classifications that can be used for different soil properties.
This kind bacterial manure is applied to indoor wheat planting experiment effect as showing:
| Fertilizer type | Leaf wide (cm) | Plant height (cm) | Multiplicity | Temperature ℃ | Moisture (%) |
| The composite bacteria garbage tankage | ????1.76 | ??49.20 | ????3 | ??15-20 | ????70 |
| The vinelandii garbage tankage | ????1.69 | ??42.31 | ????3 | ??15-20 | ????70 |
| K bacterium garbage tankage | ????1.59 | ??42.00 | ????3 | ??15-20 | ????70 |
| The phosphorus bacteria garbage tankage | ????1.60 | ??43.22 | ????3 | ??15-20 | ????70 |
| The Bacillus thuringiensis garbage tankage | ????1.59 | ??42.07 | ????3 | ??15-20 | ????70 |
| No microbial inoculum garbage tankage | ????1.49 | ??41.05 | ????3 | ??15-20 | ????70 |
| Plain soil | ????1.01 | ??30.12 | ????3 | ??15-20 | ????70 |
From as can be seen last, it is more effective than single culture that the biological organic fertilizer that contains multiple bacterial classification is applied to refuse soil.The bacterium number is all influential to percentage of germination, the growing way of wheat, the wheat 100-grain weight of wheat in the unit mass.Experiment showed, that effect is best when bacterium is counted 2.0-3.0 hundred million/g, at this moment, percentage of germination height, plant height, leaf are wide, stem girth etc. is best, and heading is neat does not have a phenomenon of delaying, and 100-grain weight is also maximum.
Except that the plantation wheat, we have also planted summer radish in the laboratory, and effect is also consistent with the wheat planting effect.Composite biological organic fertilizer can not only promote plant growth, can strengthen the crop disease-resistant ability, and in addition, mouthfeel well also is to use the characteristic of vegetables etc. behind the biological organic fertilizer.
The quality determination of composite biological organic fertilizer (as following table):
| Project | Granularity | Roundworm mortality ratio % | Colititre | ??Cd | ?Hg | ?Pb | ?As |
| The mg/kg refuse soil | |||||||
| The compost GB | ≤12 | ?95-100 | ???0.1-0.01 | ?≤3 | ?≤5 | ?≤100 | ?≤30 |
| Bio-bacterial manure | 10 | ?100 | ?1.44 | ?0.09 | ?30.15 | ?0.89 | |
| Project | Moisture % | Organic % | ???TN | ?TP | ?TK | ?pH | ?Cr |
| ???％ | ?％P 2O 5 | ?％K 2O | ?mg/kg | ||||
| The compost GB | 25-35 | ≥10 | ???≥0.5 | ?≥0.3 | ?≥1.0 | ?6.5-8.5 | ?≤300 |
| Bio-bacterial manure | 25 | 45.31 | ???1.5 | ?1.89 | ?1.52 | ?7.0 | ?63.15 |
Composite biological organic fertilizer is to the influence of soil physico-chemical property:
After the crops of experimental planting and the vegetables results, gather the content of indexs such as wherein nitrogen of soil sample test, phosphorus, potassium, organic matter, as following table:
| Soil sample | ????N(％) | ????P(10 -6) | ????K(10 -6) | Organic carbon (%) | Organic (%) |
| Cucumber ground | ????0.31 | ????83.81 | ????282.05 | ????5.11 | ????10.59 |
| Corn field | ????0.30 | ????101.02 | ????248.34 | ????5.22 | ????10.80 |
| Wheatland | ????0.31 | ????101.67 | ????267.52 | ????5.89 | ????11.18 |
| Bio-bacterial manure | ????0.30 | ????93.68 | ????352.10 | ????5.23 | ????9.92 |
| Chemical fertilizer | ????0.22 | ????61.36 | ????315.34 | ????3.47 | ????6.58 |
As can be seen from the above table, used that nitrogen, phosphorus, potassium, organic equal size all have raising in various degree in the soil of efficient composite biological organic fertilizer, and soil does not have the phenomenon of hardening, this illustrates that this efficient biologic-organic fertilizer can improve the physico-chemical property of soil, improve the fertility of soil, improve the structure of soil, help the growth of farm crop.The efficient biologic-organic fertilizer that uses domestic waste to make can obviously improve total increment and the growth increment of crop, can promote fruit early-maturing, improve the resistance against diseases of crop, has good effect of increasing production, after using efficient biologic-organic fertilizer, the content of each leading indicator such as nitrogen, phosphorus, potassium, organic matter all has raising in various degree in the soil, illustrates that this efficient biologic-organic fertilizer can improve the physico-chemical property of soil, increases soil fertility.
In sum, thus composite bacteria agent involved in the present invention has applied range, make increasing crop yield the effect of increasing income significantly and excellent results such as can make junk fully obtain utilizing to turn waste into wealth.
Come composite bacteria agent involved in the present invention is further described below in conjunction with embodiment.
Embodiment 1:
1, inclined-plane seed culture: eight kinds of bacterium connect the inclined-plane respectively, cultivate 30 hours.Respectively join 6ml then
In the sterilized water, vibrated 30 minutes, treat that evenly the back makes in every test tube each with the blood counting chamber counting
The bacterium number average of planting bacterium is 10
8Individual/ml, get bacteria suspension;
2, first order seed is cultivated: each 1ml of bacteria suspension that gets eight kinds of bacterium puts into eight 250ml triangular flasks respectively,
Put into the 100ml nutrient solution in every triangular flask respectively, wherein P bacterium, K bacterium, amylolysis
Bacterium, Bacillus thuringiensis, Bacillus licheniformis with No. 2 substratum (be made into nutrient solution with distilled water,
Down together), vinelandii are used with No. 13 substratum, cellulose-decomposing bacterium with No. 3 substratum, yeast
No. 36 substratum, 30 ℃ of cultivations of shaking table (180 rev/mins), pH value 7.2, each cultivates 48 little
The time after, with blood counting chamber method counting, by bacterium number in the unit volume than each 12.5% with eight kinds of microbial inoculums
Mix;
3, secondary seed is cultivated:, get mixed microbial inoculum 20ml and put into the 500ml triangular flask, the substratum choosing
With peptone 20 grams, NaCl 10 grams, extractum carnis 6 grams, MgSO
47H
2O 20 grams 1,
FeSO
47H
2O 0.02 gram, K
2HPO
42 grams, Na
2HPO
41 gram, Xylo-Mucine 4 gram,
CaCl
20.2 gram, sucrose 10 grams, (NH
4)
2SO
42 grams mix, and are made into 200ml with distilled water
Nutrient solution, shaking table (180 rev/mins of revolutions) was cultivated 48 hours for 30 ℃, and the pH value is 7.2,
5.5 * 10
9The composite fungus agent of individual/ml;
This composite fungus agent mixes with refuse soil, makes that the bacterium number reaches 2.67 hundred million/g in the refuse soil, and the effect of planting wheat, corn, cucumber in multiple soil property is all obvious, and volume increase reaches the effect of 10-30%, and farm crop have tangible resistance against diseases.
Embodiment 2:
1, inclined-plane seed culture: eight kinds of bacterium connect the inclined-plane respectively, cultivate 30 hours.Respectively join 6ml then
In the sterilized water, vibrated 30 minutes, treat that evenly the back makes in every test tube each with the blood counting chamber counting
The bacterium number average of planting bacterium is 10
8Individual/ml, get bacteria suspension;
2, first order seed is cultivated: each 1ml of bacteria suspension that gets eight kinds of bacterium puts into eight 250ml triangular flasks respectively,
Put into the 100ml nutrient solution in every triangular flask respectively, wherein P bacterium, K bacterium, amylolysis
Bacterium, Bacillus thuringiensis, Bacillus licheniformis with No. 2 substratum (be made into nutrient solution with distilled water,
Down together), vinelandii are used with No. 13 substratum, cellulose-decomposing bacterium with No. 3 substratum, yeast
No. 36 substratum, 30 ℃ of cultivations of shaking table (180 rev/mins), pH value 7.5 was cultivated 48 hours,
By unit volume inner cellulose decomposer, amylolysis bacterium, Bacillus licheniformis, yeast, solid
Nitrogen bacterium, P bacterium, K bacterium, Bacillus thuringiensis bacterium count ratio be respectively 10%, 15%, 15%, 10%,
20%, 10%, 10%, 10% mixed;
3, secondary seed is cultivated:, get mixed microbial inoculum 20ml and put into the 500ml triangular flask, the substratum choosing
With peptone 30 grams, NaCl 8 grams, extractum carnis 8 grams, MgSO
47H
2O 30 grams 1,
FeSO
47H
2O 0.05 gram, K
2HPO
44 grams, Na
2HPO
42 grams, Xylo-Mucine 4 gram,
CaCl
20.4 gram, sucrose 10 grams, (NH
4)
2SO
44 grams mix, and are made into 200ml with distilled water
Nutrient solution, shaking table (180 rev/mins of revolutions) was cultivated 66 hours for 30 ℃, and the pH value is 7.5,
7.9 * 10
9The composite fungus agent of individual/ml;
This composite fungus agent mixes with refuse soil, makes that the bacterium number reaches 2.84 hundred million/g in the refuse soil, and the effect of manufacturing soybean is obvious in multiple soil property, and volume increase reaches 20-35%.
Claims (3)
1, a kind of composite bacteria agent, the bacterium number is such as following in its composition bacterial classification and the unit volume: cellulose-decomposing bacterium 10-30%, amylolysis bacterium 10-30%, Bacillus licheniformis 10-30%, yeast 10-30%, vinelandii 10-20%, P bacterium 10-30%, K bacterium 10-20%, Bacillus thuringiensis 10-30%.
2, a kind of cultural method of composite bacteria agent as claimed in claim 1, its step is as follows:
1. inclined-plane seed culture: eight kinds of bacterium connect the inclined-plane respectively, cultivate 24-36 hour, respectively join then in the sterilized water, vibrate 30 minutes, treat evenly the back with the blood counting chamber counting, and the bacterium number average that makes various bacterium in every test tube is 10
8Individual/ml, get bacteria suspension;
2. first order seed is cultivated: each 1ml of the bacteria suspension of eight kinds of bacterium puts into eight 250ml triangular flasks respectively, put into the 100ml nutrient solution in every triangular flask respectively, wherein P bacterium, K bacterium, amylolysis bacterium, Bacillus thuringiensis, Bacillus licheniformis are with No. 2 substratum, vinelandii are with No. 3 substratum, yeast was cultivated 36-72 hour in 30 ℃ with No. 36 substratum, 180 rev/mins of shaking tables with No. 13 substratum, cellulose-decomposing bacterium, the pH value is 7.0-7.5, makes in every triangular flask bacterium count content and reaches 10
8Individual/ml, by bacterium number ratio in the unit volume described in the claim 1 eight kinds of microbial inoculums are mixed;
3. secondary seed is cultivated: get mixed microbial inoculum and put into the 500ml triangular flask, connecing the bacterium amount is 10%, and substratum is selected peptone 5-15 gram/100ml, NaCl 2-8 gram/100ml, extractum carnis 1-5 gram/100ml, MgSO for use
47H
2O 5-15 gram/100ml, FeSO
47H
2O 0.01-0.05 gram/100ml, K
2HPO
40.5-3 gram/100ml, Na
2HPO
40.1-1 gram/100ml, Xylo-Mucine 0.5-5 gram/100ml, CaCl
20.05-0.5 gram/100ml, sucrose 2-8 gram/100ml, (NH
4)
2SO
40.5-3 gram/100ml mixes, all the other are distilled water, and 180 rev/mins of shaking tables were in 30 ℃ of cultivations 36-72 hour, and the pH value is 7.0-7.5, makes the bacterium number reach 3-8 * 10
9Individual/ml, promptly get composite fungus agent.
3, the application of a kind of composite bacteria agent as claimed in claim 1 aspect the preparation bio-bacterial manure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00106372 CN1274702A (en) | 2000-06-14 | 2000-06-14 | Composite biological bacteria preparation and its preparation process and application in preparing bacterial manure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00106372 CN1274702A (en) | 2000-06-14 | 2000-06-14 | Composite biological bacteria preparation and its preparation process and application in preparing bacterial manure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1274702A true CN1274702A (en) | 2000-11-29 |
Family
ID=4578323
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|---|---|---|---|
| CN 00106372 Pending CN1274702A (en) | 2000-06-14 | 2000-06-14 | Composite biological bacteria preparation and its preparation process and application in preparing bacterial manure |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101311259B (en) * | 2007-05-24 | 2010-10-06 | 成都合成生物科技有限公司 | Microorganism decomposition agent and method for preparing same |
| CN102511291A (en) * | 2011-12-27 | 2012-06-27 | 兰州大学 | Method for planting Populus euphratica in heavy saline-alkaline land of Gobi desert |
| CN103030441A (en) * | 2012-12-31 | 2013-04-10 | 天津北洋百川生物技术有限公司 | Novel pest-resistant liquid compound microbial fertilizer |
| CN103159511A (en) * | 2011-12-16 | 2013-06-19 | 河北农业大学 | Bacillus thuringiensis solid fermentation process and application thereof in agriculture |
| CN103952360A (en) * | 2014-05-09 | 2014-07-30 | 中国农业科学院北京畜牧兽医研究所 | Cleaning agent for environment in livestock house and application thereof |
| CN105130684A (en) * | 2015-09-29 | 2015-12-09 | 闽南师范大学 | Formula of compound biological bacterial fertilizer and preparation process and application thereof |
-
2000
- 2000-06-14 CN CN 00106372 patent/CN1274702A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101311259B (en) * | 2007-05-24 | 2010-10-06 | 成都合成生物科技有限公司 | Microorganism decomposition agent and method for preparing same |
| CN103159511A (en) * | 2011-12-16 | 2013-06-19 | 河北农业大学 | Bacillus thuringiensis solid fermentation process and application thereof in agriculture |
| CN102511291A (en) * | 2011-12-27 | 2012-06-27 | 兰州大学 | Method for planting Populus euphratica in heavy saline-alkaline land of Gobi desert |
| CN102511291B (en) * | 2011-12-27 | 2013-09-11 | 兰州大学 | Method for planting Populus euphratica in heavy saline-alkaline land of Gobi desert |
| CN103030441A (en) * | 2012-12-31 | 2013-04-10 | 天津北洋百川生物技术有限公司 | Novel pest-resistant liquid compound microbial fertilizer |
| CN103030441B (en) * | 2012-12-31 | 2014-06-25 | 天津北洋百川生物技术有限公司 | Novel pest-resistant liquid compound microbial fertilizer |
| CN103952360A (en) * | 2014-05-09 | 2014-07-30 | 中国农业科学院北京畜牧兽医研究所 | Cleaning agent for environment in livestock house and application thereof |
| CN105130684A (en) * | 2015-09-29 | 2015-12-09 | 闽南师范大学 | Formula of compound biological bacterial fertilizer and preparation process and application thereof |
| CN105130684B (en) * | 2015-09-29 | 2018-09-18 | 闽南师范大学 | The formula and manufacture craft of a kind of composite biological fertilizer and application |
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