CN1032840C - Production-increasing compounded bacterium and its production method - Google Patents
Production-increasing compounded bacterium and its production method Download PDFInfo
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Abstract
The present invention relates to a high-efficiency yield-increasing bacillus preparation and a production method thereof, particularly to single bacterium preparations prepared from waxiness bacillus, bacillus brevis, bacillus firmus, bacillus subtilis, bacillus sphaericus and bacillus licheniformis and mixed bacterium preparations prepared from waxiness bacillus and bacillus brevis, waxiness bacillus and bacillus firmus, waxiness bacillus and bacillus subtilis, waxiness bacillus and bacillus sphaericus, waxiness bacillus and bacillus licheniformis, waxiness bacillus and waxiness bacillus, waxiness bacillus and bacillus licheniformis, and bacillus firmus and bacillus subtilis, and a production method. The yield-increasing bacillus has obvious effect on increasing yield and preventing diseases.
Description
Human for protecting the struggle that crops carried out to run through the agricultural history in nearly ten thousand years.Along with the development process of agricultural modernization, people constantly are subjected to the inspiration that each field science and technology is maked rapid progress, and meanwhile, the various crises that the mankind face are also more outstanding.What stand in the breach is exactly energy crisis, as the agricultural of the reviviscence energy, becomes the high energy input coefficient day by day owing to the deep of modernization, intensification.Energy scarcity makes this agricultural be difficult to development and goes down, and particularly in third world countries, what be badly in need of development is that lower energy is imported and the agricultural system of high yield.The measure of taking this century is breed improvement, enrich the fertilizer and the extermination of disease and insect pest.
The use of a large amount of chemical fertilizer, agricultural chemicals needs a large amount of inputs of energy on the one hand, has brought environmental pollution and ecological balance damage on the other hand again, and people recall past pains, then biotechnology is expressed very big hope.
The screening microorganism is used for crops diseases prevention, health care and volume increase, owing to have safety non-pollution, produce easy, with low cost and advantage such as effect is remarkable, become noticeable field in the agronomy research day by day.Up to now, it is microbe-derived in the different ecological environment that screening utilizes, and comprises each monoid such as bacterium, actinomycetes, fungi and saccharomycete.
Utilize the bacterial treatment crops with the existing big quantity research of the purpose that reaches disease-preventing and yield-increasing,, mainly contain the three major types type according to the character of used bacterium.One class is azotobacter, and except symbiotic nitrogen-fixing bacterias such as rhizobium, gramineous crop rhizosphere combination azotobacter and soil azotobacter successively have application in various degree at home and abroad; One class is anti-germ, mainly is to utilize the bacterium that pathogen is had antagonism; Another kind of is the short living bacterium that European and American countries is paid attention to research after the seventies, promptly screens the rhizosphere bacteria that may produce antibiotic from plant rhizosphere again, mainly to liking false pseudomonas bacillus.The practical application scale of back two class bacterium can not show a candle to solid ammonia bacterium.
The present inventor is in the process of studying for a long period of time, founded the new technology path that utilizes bacterium volume increase diseases prevention: (1) inventor research thinks that the interior microorganism of plant surface body has formed the ecological relationship of interdependence mutual restriction with plant corpus in the long-term evolution process, they and plant are formed a symbiosis complex, i.e. " natural ecosystem "; (2) volume increase and diseases prevention are depended on this holocoenotic comprehensive reformation and adjusting effectively, rather than to the independent effect of indivedual pathogens; (3) some beneficial microbes that filter out from plant corpus itself, often have with the plant corpus compatibility strong, characteristics such as effect is lasting and comprehensively work in plant surface, body, thereby be expected in agricultural production practice, to obtain even more ideal and stable effect.The representative of this respect is exactly the research and the application (patent No. 88106560.9) of crops output increasing bacteria, the waxy Bacillus (Bacillus cereus) that adopts this technology path to obtain from the plant corpus screening, have and efficiently, stably promote crop yield, prevent and treat crop pest and improve crop product quality etc. multi-functional, in agricultural production, obtained huge social, economy and ecological benefits.
The objective of the invention is; Utilize the method for separation screening bacterial classification of the applicant's patent 88106560, filter out can same plant symbiosis for body surface in the plant corpus, and can promote the bacillus of crop yield diseases prevention, utilize the gained bacillus to produce single then or mixing crop yield bacteria agent, this microbial inoculum is applied in the purpose that reaches volume increase, diseases prevention on the crops.
The invention is characterized in: the applicant utilizes the separating screening method of the patent No. 88106560, and body surface has separated waxy Bacillus (Bacillus cereus) MEP-01 (CGMCC0188-01) in the plant corpus, bacillus brevis (Ba-cillus brevis) MEP-02 (CGMCC0188-02), bacillus firmus (Bacillus firmus) MEP-03 (CGMCC0188-03), bacillus subtilis (Bacillus subtilis) MEP-04 (CGMCC0188-04), Bacillus sphaericus (Bacillus sphaericus) MEP-05 (CGMCC0188-05), six kinds of bacillus of bacillus licheniformis (Bacillus lichenformis) MEP-06 (CGMCC0188-06).The applicant has filtered out YIB waxy Bacillus (Bacillus cereus) when application on September 28th, 1,988 88106560 patents in addition.
The present invention utilizes above-mentioned bacillus to produce single gemma bacillus agent product or production mixing gemma bacillus agent product, and these microbial inoculum products are applied in has the effect that promotes crop yield and diseases prevention significantly on the crops.
Totally six kinds of the single gemma bacillus agent products of producing, they are:
1, waxy Bacillus (Bacillus cereus) MEP-01 microbial inoculum
2, bacillus brevis (Bacillus brevis) MEP-02 microbial inoculum
3, bacillus firmus (B.firmus) MEP-03 microbial inoculum
4, bacillus subtilis (B.subtilis) MEP-04 microbial inoculum
5, Bacillus sphaericus (B.sphaeficus) MEP-05 microbial inoculum
6, bacillus licheniformis (B.licheniformis) MEP-06 microbial inoculum
Totally eight kinds of the mixing gemma bacillus agent products of producing, they are:
1, waxy Bacillus YIB and waxy Bacillus MEP-01 mix bacterium agent
2, waxy Bacillus YIB and bacillus brevis MEP-02 mix bacterium agent
3, waxy Bacillus YIB and bacillus firmus MEP-03 mix bacterium agent
4, the mix bacterium agent of waxy Bacillus YIB and bacillus subtilis MEP-04
5, the mix bacterium agent of waxy Bacillus YIB and Bacillus sphaericus MEP-05
6, the mix bacterium agent of waxy Bacillus YIB and bacillus licheniformis MEP-06
7, the mix bacterium agent of waxy Bacillus MEP-01 and bacillus brevis MEP-02
8, the mix bacterium agent of bacillus firmus MEP-03 and bacillus subtilis MEP-04
Produce above-mentioned mix bacterium agent and contain bacterium than two kinds of bacterial classifications are made into mixed bacteria liquid by following: its proportioning is respectively:
Waxy Bacillus YIB and waxy Bacillus MEP-01 are 1: 0.5-2
Waxy Bacillus YIB and bacillus brevis MEP-02 are 1: 0.5-1.5
Waxy Bacillus YIB and bacillus firmus MEP-03 are 1: 0.5-1.5
Waxy Bacillus YIB and bacillus subtilis MEP-04 are 1: 0.5-2
Waxy Bacillus YIB and Bacillus sphaericus MEP-05 are 1: 0.5-2
Waxy Bacillus YIB and bacillus licheniformis MEP-06 are 1: 0.5-1.5 waxy Bacillus MEP-01 and bacillus brevis MEP-02 are 1: 0.5-1.5
Bacillus firmus MEP-03 and bacillus subtilis MEP-04 are 1: 0.5-2
Above-mentioned mixed bacteria liquid is inoculated into seeding tank respectively and sends out in the composts or fertilisers of cultivating of alcohol jar and ferment, all can obtain crops output increasing bacteria mix bacterium agent product.
The basic biological property of bacterial strain
MEP-01, waxy Bacillus (Bacillus cereus).Thalline is shaft-like, and is long 3～5 microns, wide 1.0～1.2 microns, the Gram-reaction positive, before gemma forms in its protoplasm not colored particles be beta-hydroxy-butanoic acid salt.Give birth in the gemma or nearly middle giving birth to, ellipse or column, sporangium is not expanded, energy anaerobic growth, V.P reacting positive, V.P liquid pH4.3-5.5: growth temperature 10-45 ℃, grow among the 7%NaCl; PH5.7 grows, and produces acid from glucose, maltose, does not produce acid from wood sugar, lactose, mannitol, and the energy hydrolyzed starch utilizes citrate; Reductive NO
3 -→ NO
2 -
MEP-02, bacillus brevis (Bacillus brevis).Thalline is shaft-like, and is long 1.5～4 microns, wide 0.6～0.9 micron.Gram-reaction is positive or variable, gemma ellipse or column, and end is given birth to or near-end is given birth to, and sporangium is expanded, not from the carbohydrate aerogenesis, can not hydrolyzed starch, the catalase positive; In glucose nutrient broth culture fluid, grow back pH greater than 8.0, V.P reacting positive, V.P liquid pH8.0-8.6; Growth temperature 10-60 ℃, 7%NaCl does not grow, pH5.7 growth, phenyl alanine deamination feminine gender, the nitrate reduction positive.
MEP-03, bacillus firmus (B.firmus).Thalline is shaft-like, and long 3-4 micron is wide 0.8～1.0 micron, the Gram-reaction positive, gemma ellipse, middle life or nearly middle giving birth to, sporangiocyst does not expand, and does not contain not colored particles, the catalase positive in the protoplasm, can not anaerobic growth, the V-P reaction negative is grown among the 7%NaCl, and pH5.7 does not grow, the energy hydrolyzed starch, reduction nitrate is nitrite.
MEP-04, bacillus subtilis (B.subtilis).Thalline is shaft-like, long 2-3 micron, wide 0.7-0.8 micron, the Gram-reaction positive, gemma ellipse or column, middle life or nearly middle giving birth to, sporangiocyst does not expand, do not contain the beta-hydroxy-butanoic acid salt particle in the protoplasm: grow among the 7%Nal, stone core milk does not produce acid, pH5.7 growth, the V.P positive, hydrolyzed starch, reductive NO
3 -→ NO
2 -, can not anaerobic growth, the catalase positive.
MEP-05, Bacillus sphaericus (B.sphaeriaus).Thalline is shaft-like, and long 1.5～5.0 microns, wide 0.6～1.0 micron, Gram-reaction is variable, the gemma sphere, and end is given birth to or near-end is given birth to, and sporangiocyst expands, and hydrolyzed starch does not produce acid, the catalase positive, the phenyl alanine deamination positive, V.P reaction negative from carbohydrate fermentation.MEP-06, bacillus licheniformis (B.lichenformis).The thalline direct rod shape, chaining, long 2.5～3.5 microns, wide 1～1.2 micron, the Gram-reaction positive, gemma ellipse or column, sporangiocyst does not expand, and does not have not painted bead in the protoplasm.Hydrolyzed starch utilizes citrate, and the nitrate that reduces becomes nitrite, and carbohydrate is aerogenesis not, 7%NaCl growth, pH5.7 growth, the V.P positive, V.P liquid growth back pH6.3, energy anaerobic growth, the catalase positive.
Above-mentioned six bacterial strains have been deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on January 15th, 1993.
YIB, waxy Bacillus (Bacillus cereus).Long 2.5-3.5 micron under thalline direct rod shape, chaining, the light microscopic, wide 1 micron, the Gram-reaction positive, gemma ellipse or column, middle giving birth to, sporangiocyst does not expand, and protoplasm contains not colored particles, agar is dull and stereotyped to be cultivated, the bacterium colony circle, diameter 0.2cm, neat in edge, swell slightly, tarnish, opaque, wax; Liquid culture forms mycoderm.Growth temperature 15-45 ℃, the suitableeest 27-32 ℃, appropriate pH 6.0-7.5, suitable carbon source maltose, sucrose, suitable nitrogen source are peptone, inorganic nitrogen-sourced is NH
4 +, glucose fermentation, maltose produces acid, and wood sugar, lactose, mannitol do not produce acid.Hydrolyzed starch utilizes citrate, and reduction nitrate becomes nitrite, grow among the 7%NaCl, and the pH5.7 growth, the V.P positive, V.P liquid is cultivated back pH4.56.The energy anaerobic growth, the catalase positive, carbohydrate aerogenesis feminine gender, edwardsiella hoshinae, phenyl alanine deamination feminine gender.
The YIB bacterial strain is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 28th, 1988, registers on the books and be numbered CGMCC0137 in the preservation center.
Fermentation manufacturing technique
Comprise: actication of culture → bacterial classification enlarged culture → bacterium liquid preparation → seeding tank is sent out processing → product inspection → packing behind alcohol → the send out alcohol jar fermentation → pulvis
(1) bacterial classification is prepared
Bacterial classification changes flat bottle over to and cultivated 24 hours at 28～30 ℃ after gravy peptone slant activation, makes suspension with 0.1% peptone water solution.Fully sampling is counted behind the vibration mixing.Be mixed and made into mixed bacteria liquid separately or in proportion with preparing to send out the pure bacterial classification of producing, be used for seed tank culture.
(2) seed tank culture
Seeding tank prescription: soybean cake powder 1-37%, fish meal 1%, corn steep liquor 0.3%, glucose 0.2%, dried silkworm chrysalis meal 0.3%, soya-bean oil 0.2-0.3%.Regulate pH value 7.4-7.5.
Slack tank was sterilized 30 minutes under 125 ℃/0.14MPa pressure, sterilized 30 minutes under 121 ℃/0.11MPa in the charging back.Be cooled to 35 ℃ of inoculations, per 350 liter seeding tanks connect 4-5 flat bottle.The inoculation back is 30～34 ℃ of temperature, and throughput (v.v.m) condition under is cultivated 5-7 hour at 1: 1.1, gets final product culture transferring to sending out alcohol jar interior fermented and cultured.This moment, seeding tank zymotic fluid bacterium amount reached 5～1,000,000,000/ml.
(3) ferment tank
Fermentation tank was sterilized 30 minutes through 121 ℃/0.11MPa and 125 ℃/0.14MPa respectively before inoculation and before feeding intake.
Fermentation tank prescription: groundnut meal 1%, soybean cake powder 1%, fish meal 0.5%, corn steep liquor 1%, dextrin 0.8%, magnesium sulfate 0.1%, calcium carbonate 0.15%, KH
2PO
40.02%, soya-bean oil 0.15%, pH value 7.4-7.6.
Inoculation when composts or fertilisers of cultivating is cooled to 35 ℃, inoculum concentration is 3-5%.Cultivation temperature: 32～34 ℃ of early stages, after 12 hours 35-36 ℃.Throughput: inoculate in back 6 hours 1: 1, increase to 1: 1 after 6 hours.Fermentation time 16～2.0 hours, bacteria containing amount reaches 50～8,000,000,000/ml.
(4) process behind the pulvis
Zymotic fluid can directly be bottled and is packaged into microbial inoculum.Zymotic fluid also can be processed into pulvis.The processing powder agent method is, adds the absorption compound earlier, mechanical agitation 4-5 hour, and after the bacterium spore is adsorbed entirely, the flame filter press press filtration; Filter cake is put drying machine drying (bake out temperature is no more than 70 ℃) after smashing to pieces, when moisture content≤5%, promptly pulverize, and its granularity requirements is by the 0.09mm aperture sieve.The powder product bacteria containing amount is not less than 50,000,000,000/gram, can pack after check.
The application method of microbial inoculum
Microbial inoculum can be used for seed-soaking, seed dressing, foliar spray or is stained with root, and using method is with crops output increasing bacteria YIB, but this liquid preparation consumption only needs 1/2～1/4 of YIB, and the pulvis consumption only needs 1/5 of YIB.
Advantage of the present invention and good effect
(1) fermented product of bacterial strain MEP-01, MEP-02, MEP-05 is exclusively used in dicotyledonous class crop; The fermented product of bacterial strain MEP-03, MEP-04, MEP-06 is exclusively used in the monocotyledons crop.Their production-increasing function is not less than the effect of bacterial strain YIB on dicotyledonous and monocot crops respectively.Bacterial strain YIB and MEP-01, YIB and MEP-02, the fermented product of YIB and MEP-05 and MEP-01 and MEP-02 is exclusively used in dicotyledonous class crop; YIB and MEP-03, YIB and MEP-04, YIB and MEP-08 and MEP-03 and MEP-04 fermented product are exclusively used in single leaf class crop.They obviously are better than novel yield increasing fungus and crops output increasing bacteria YIB to dicotyledonous and the effect of increasing production monocotyledons crop, the stable volume increase of general dicotyledonous crops 25-120%, the stable volume increase of monocot crops 20%-90%.Wherein YIB+MEP-02 is particularly suited for fruit tree, and except that increasing production and increasing the sugar, effects such as the mould worry of control apple, ring spot reach more than 65%; MEP-01+MEP-02 is particularly suited for beet, and except that efficient volume increase, sugar content improves more than 2 degree.YIB+MEP-04 is particularly suited for paddy rice and wheat, except that remarkable volume increase, prevents and treats the banded sclerotial blight effect and reaches more than 70%.
(2) for the solid fermentation process of novel yield increasing fungus, the not only water soluble application such as foliar spray of being more convenient for of the formed powder product of the present invention, and also bacteria containing amount improved more than five times, not only improved effect, also is convenient to transportation and preservation.
(3) for the zymotechnique of yield increasing fungus YIB, bacteria containing amount of the present invention is improved largely, and has saved cost, has improved benefit.
Narrate embodiments of the invention below:
The inventor of crops output increasing bacteria is on the basis of further investigation, the useful waxy Bacillus that discovery filters out in the monocotyledon body surface body and mix from the bacillus licheniformis that the dicotyledon screening obtains and to use, have the synergistic effect that is better than the independent use of single strain, and studied its solid fermentation process (publication number 90103591.2).The present inventor finds in further research process: different classes of bacillus has in various degree common point and otherness to the effect of plant in (1) plant surface body: (2) useful Bacillus strain of gained from the similar plant is used effect with and often is better than on the dissimilar plants resulting bacterial strain and uses with.Therefore, the present inventor improves and has set up the compound method of efficient increasing production by compounding bacterium, and has studied and improved corresponding liquid zymotechnique and pulvis process technology, is described below now:
One, yield increasing fungus product embodiments:
Six kinds of bacillus that the present invention separates can be produced single gemma bacillus agent and be mixed the gemma bacillus agent product:
Totally six kinds of the single gemma bacillus agent products of producing, they are:
1, waxy Bacillus (Bacillus cereus) MEP-01 microbial inoculum
2, bacillus brevis (Bacillus brevis) MEP-02 microbial inoculum
3, bacillus firmus (B.firmus) MEP-03 microbial inoculum
4, bacillus subtilis (B.subtilis) MEP-04 microbial inoculum
5, Bacillus sphaericus (B.sphaencus) MEP-05 microbial inoculum
6, bacillus licheniformis (B.licheniformis) MEP-06 microbial inoculum
Totally eight kinds of the mixing gemma bacillus agent products of producing, they are:
1, the mix bacterium agent of waxy Bacillus YIB and waxy Bacillus MEP-01
2, the mix bacterium agent of waxy Bacillus YIB and bacillus brevis MEP-02
3, the mix bacterium agent of waxy Bacillus YIB and bacillus firmus MEP-03
4, the mix bacterium agent of waxy Bacillus YIB and bacillus subtilis MEP-04
5, the mix bacterium agent of waxy Bacillus YIB and Bacillus sphaericus MEP-05
6, the mix bacterium agent of waxy Bacillus YIB and bacillus licheniformis MEP-06
7, the mix bacterium agent of waxy Bacillus MEP-01 and bacillus brevis MEP-02
8, the mix bacterium agent of bacillus firmus MEP-03 and bacillus subtilis MEP-04
Two, efficient increasing production by compounding bacterium production method embodiment:
Embodiment 1
(1) spawn culture
Respectively after gravy peptone slant activation, change YIB and MEP-01 over to flat bottle, cultivated 24 hours at 28～30 ℃, adding concentration is 0.1% peptone water solution, makes bacteria suspension, and mixing fully vibrates, behind the counting suspension is counted the ratio mixing in 1: 1 bacterium, be used to inoculate seeding tank.
(2) seed tank culture
Seed tank culture liquid partition: soybean cake powder 1.5%, fish meal 1%, corn steep liquor 0.3%, glucose 0.2%, dried silkworm chrysalis meal 0.3%, soya-bean oil 0.25%, regulate pH value 7.5, with the inoculum concentration inoculation mixed bacteria liquid of 4 flat bottles of per 350 liter culture fluids inoculation (2 flat bottles of every bacterium) bacterial classification gained bacterium liquid.30～34 ℃ of cultivation temperature.Throughput is 1: 1 (v.v.m), cultivates 5-7 hour.This moment, seeding tank zymotic fluid bacterium amount reached 5～1,000,000,000/ml.
(3) ferment tank
Fermentation tank culture formula of liquid: groundnut meal 1%, soybean cake powder 1%, fish meal 0.5%, corn steep liquor 1%, dextrin 0.8%, magnesium sulfate 0.1%, calcium carbonate 0.15%, KH
2PO
40.02%, soya-bean oil 0.15%, pH value 7.5.Inoculum concentration inoculation seeding tank zymotic fluid with 3%.Cultivation temperature: 32-34 ℃ of early stage, after 12 hours 35-36 ℃.Throughput: inoculate 1: 1 (v.vm) in back 6 hours, increase to 1: 1.1 after 6 hours (v.vm).Fermentation time 16-20 hour, bacteria containing amount reached 7,500,000,000/ml.Zymotic fluid directly bottling is packaged into liquid bacterial agent.
(4) process behind the pulvis
Zymotic fluid also can be processed into pulvis.The processing powder agent method is, adds the absorption compound earlier, mechanical agitation 4-5 hour, and after the bacterium spore is adsorbed entirely, the flame filter press press filtration; Filter cake is put drying machine drying (bake out temperature is no more than 70 ℃) after smashing to pieces, when moisture content≤5%, promptly pulverize, and its granularity requirements is by the 0.09mm aperture sieve.The powder product bacteria containing amount is not less than 50,000,000,000/gram, can pack after check.
Embodiment 2
Basically adopt embodiment 1 same procedure.Difference is to adopt YIB and MEP-02 bacterial classification with 1: the ratio combined inoculation seeding tank of 1.2-1.5.
Embodiment 3
Basically adopt and embodiment 1 same procedure.Difference is to adopt YIB and MEP-03 bacterial classification with 1: the ratio of 1.2-1.5 is mixed back inoculation seeding tank.
Embodiment 4
Basically adopt and embodiment 1 same procedure.Difference is to adopt YIB and MEP-04 bacterial classification with 1: the ratio of 1.2-1.5 is mixed back inoculation seeding tank.
Embodiment 5
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-01 and MEP-02 with 1: the ratio of 1.2-1.5 is mixed back inoculation seeding tank.
Embodiment 6
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-03 and MEP-04 bacterial classification to mix back inoculation seeding tank with 1: 1 ratio.
Embodiment 7
Basically adopt and embodiment 1 same procedure.Difference is to adopt YIB and MEP-05 to mix back inoculation seeding tank with 1: 1 ratio.
Embodiment 8
Basically adopt and embodiment 1 same procedure.Difference is to adopt YIB and MEP-06 to mix back inoculation seeding tank with 1: 1 ratio.
Embodiment 9
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-01 bacterial classification inoculation seeding tank.
Embodiment 10
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-02 bacterial classification inoculation seeding tank.
Embodiment 11
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-03 bacterial classification inoculation seeding tank.
Embodiment 12
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-04 bacterial classification inoculation seeding tank.
Embodiment 13
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-05 bacterial classification inoculation seeding tank.
Embodiment 14
Basically adopt and embodiment 1 same procedure.Difference is to adopt MEP-06 bacterial classification inoculation seeding tank.
Claims (11)
1, crops output increasing bacteria by single fermentation of bacillus, it is characterized in that: this crops output increasing bacteria is by waxy Bacillus (Bacil-lus cereus) MEP-01 (CGMCC0188-01), perhaps bacillus brevis (Bacillus brevis) MEP-02 (CGMCC0188-02), perhaps bacillus firmus (Bacil-lus firmus) MEP-03 (CGMCC0188-03), perhaps bacillus subtilis (Bacillus subtilis) MEP-04 (CGMCC0188-04), perhaps Bacillus sphaericus (Bacil-lus sphaericus) MEP-05 (CGMCC0188-05), the perhaps microbial inoculum of bacillus licheniformis (Bacillus licheniformis) MEP-06 (CGMCC0188-06) single fermentation gained.
2, by the crops output increasing bacteria that mixes fermentation of bacillus, it is characterized in that: this crops output increasing bacteria is by waxy Bacillus YIB (CGMCC0137) and MEP-01, perhaps YIB and MEP-02, perhaps YIB and MEP-03, perhaps YIB and MEP-04, perhaps YIB and MEP-05, perhaps YIB and MEP-06, MEP-01 and MEP-02, perhaps MEP-03 and MEP-04, the microbial inoculum of mixed culture fermentation gained.
3, a kind of production method of crop yield bacteria agent comprises actication of culture, bacterial classification enlarged culture, the preparation of bacterium liquid, seeding tank fermentation, ferment tank; Process behind the pulvis, product inspection, packing, it is characterized in that: be made into mixed bacteria liquid in following ratio when producing the mixed production increasing bacteria agent: YIB and MEP-01 ratio are 1: 0.5-2, YIB and MEP-02 ratio are 1: 0.5-1.5, YIB and MEP-03 ratio are 1: 0.5-1.5, YIB and MEP-04 ratio are 1: 0.5-2, YIB and MEP-05 ratio are 1: 0.5-2, YIB and MEP-06 ratio are 1: 0.5-1.5, MEP-01 and MEP-02 ratio are 1: 0.5-1.5, MEP-03 and MEP-04 ratio are 1: 0.5-2 is inoculated into mixed bacteria liquid respectively that fermentation all can obtain crops output increasing bacteria mix bacterium agent product in the composts or fertilisers of cultivating of seeding tank and fermentation tank.
4, production method according to claim 3 is characterized in that: YIB and MEP-01 ratio are 1: 1.
5, production method according to claim 3 is characterized in that: YIB and MEP-02 ratio are 1: 1.2-1.5.
6, production method according to claim 3 is characterized in that: YIB and MEP-03 ratio are 1: 1.2-1.5.
7, production method according to claim 3 is characterized in that: YIB and MEP-04 ratio are 1: 1.2-1.5.
8, production method according to claim 3 is characterized in that: MEP-01 and MEP-02 ratio are 1: 1.2-1.5.
9, production method according to claim 3 is characterized in that: MEP-03 and MEP-04 ratio are 1: 1.
10, production method according to claim 3 is characterized in that: YIB and MEP-05 ratio are 1: 1.
11, production method according to claim 3 is characterized in that: YIB and MEP-06 ratio are 1: 1.
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| CN100401897C (en) * | 2004-05-13 | 2008-07-16 | 南京农业大学 | Composite fungus agent for preventing blight of vegetable in greenhouse |
| CN101899405B (en) * | 2010-01-20 | 2012-04-25 | 辛伟成 | Bacillus subtilis GU and application in preparing efficient biological compound phosphate fertilizer thereof |
| CN102302040A (en) * | 2011-06-28 | 2012-01-04 | 海南博士威农用化学有限公司 | Seedling raising intelligent bacterium block containing bacillus subtilis or bacillus cereus and preparation method thereof |
-
1993
- 1993-02-19 CN CN 93101635 patent/CN1032840C/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103952360A (en) * | 2014-05-09 | 2014-07-30 | 中国农业科学院北京畜牧兽医研究所 | Cleaning agent for environment in livestock house and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1091590A (en) | 1994-09-07 |
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