CN111197011A - Bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of livestock farm - Google Patents
Bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of livestock farm Download PDFInfo
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- 241000563903 Bacillus velezensis Species 0.000 title claims abstract description 51
- 230000002265 prevention Effects 0.000 title claims abstract description 25
- 244000144972 livestock Species 0.000 title claims abstract description 23
- 238000004332 deodorization Methods 0.000 title claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 244000144977 poultry Species 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 claims description 13
- 230000001877 deodorizing effect Effects 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 13
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- 239000001888 Peptone Substances 0.000 claims description 6
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- 229940041514 candida albicans extract Drugs 0.000 claims description 6
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- 239000012138 yeast extract Substances 0.000 claims description 6
- 239000002781 deodorant agent Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
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- 238000001914 filtration Methods 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 238000000108 ultra-filtration Methods 0.000 claims description 4
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 3
- 239000001099 ammonium carbonate Substances 0.000 claims description 3
- 235000012501 ammonium carbonate Nutrition 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 3
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- 239000000126 substance Substances 0.000 abstract description 12
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- 230000000694 effects Effects 0.000 abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 4
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- 241000287828 Gallus gallus Species 0.000 description 9
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 6
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- -1 carbon methyl compound Chemical class 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
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- 238000011109 contamination Methods 0.000 description 2
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- 230000007613 environmental effect Effects 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
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- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 108010026389 Gramicidin Proteins 0.000 description 1
- 241000606856 Pasteurella multocida Species 0.000 description 1
- 108010040201 Polymyxins Proteins 0.000 description 1
- 241000607683 Salmonella enterica subsp. enterica serovar Pullorum Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
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- ZWCXYZRRTRDGQE-SORVKSEFSA-N gramicidina Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@H](NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC=O)C(C)C)CC(C)C)C(=O)NCCO)=CNC2=C1 ZWCXYZRRTRDGQE-SORVKSEFSA-N 0.000 description 1
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- 238000009629 microbiological culture Methods 0.000 description 1
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- 229960000988 nystatin Drugs 0.000 description 1
- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 1
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- 241000712461 unidentified influenza virus Species 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2258/00—Sources of waste gases
- B01D2258/02—Other waste gases
- B01D2258/0266—Other waste gases from animal farms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Virology (AREA)
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- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention relates to a bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of an animal farm, which is used as a liquid spray for the first time and applied to the animal farm, the strain has low nutritional requirement, strong stress resistance, and rapid growth and propagation, can rapidly grow and fix after being sprayed to the environment, and metabolize to generate various antibacterial substances, various enzymes, and the like, thereby having antagonism, inhibition or killing effect on pathogenic bacteria, achieving the purposes of air treatment in the culture environment of disinfection, deodorization, purification and epidemic prevention, and it has the characteristics of safety to human and livestock, less environmental pollution, strong pertinence to the prevention and treatment objects, no side effect, good effect, and the like, thereby reducing the use of chemical deodorization epidemic prevention agents which are adopted all the time at home and abroad.
Description
Technical Field
The invention relates to a bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of an animal farm, belonging to the technical field of microorganisms.
Background
Epidemic diseases and common diseases of livestock and poultry caused by pathogenic bacteria are one of the main causes of the loss of the breeding industry in the world. Moreover, most of the modern large-scale livestock and poultry are raised in closed colony houses, pathogenic bacteria contained in excrement and secretion of the livestock and poultry cause air odor and pathogen pollution to induce frequent mass diseases of the livestock and poultry, and people mainly adopt chemical disinfectants to prevent and treat the diseases and treat odor for half a century, but the effect is very little. On the other hand, many chemical disinfectants have the characteristics of high toxicity, high residue, difficult degradation, secondary pollution and the like, and with the long-term unreasonable use of the chemical disinfectants, the environmental pollution is serious day by day, and the health of people and livestock is greatly threatened. In addition, the prevention and treatment efficiency of the chemical disinfectant is gradually reduced due to the continuous occurrence of drug-resistant germs. The use amount and the use frequency are continuously increased, so that the residual quantity of the chemical disinfectant is gradually increased, and the chemical disinfectant has greater harm to the safety of people and livestock.
In recent years, with the emergence of various livestock influenza epidemics, the trend that livestock influenza viruses are spread to human beings after variation gradually appears, and the most easily spread way, namely air spread, still has no better sterilization prevention way at present.
Odor refers to all gaseous substances that irritate the human olfactory organs, cause unpleasant sensations, and damage the living environment. Common odor contaminants include ammonia, hydrogen sulfide, methyl mercaptan, and the like. These odor contaminations are the second largest hazard to farm contamination next to pathogenic bacteria. At present, an efficient and safe biological deodorant is not available, particularly, an effective treatment method for methyl mercaptan odor is not available, and bacillus methylotrophicus can just utilize the odor as a nutrient substance for self growth to absorb, convert and degrade.
Bacillus methylotrophicus LW-6(Bacillus methylotrophicus) is a gram-positive bacterium that can grow using a single carbon methyl compound as a carbon and energy source. The monocarbon compound is a compound containing only one carbon atom in the molecule, such as carbon monoxide, carbon dioxide, methane, methanol and the like, the bacterium can change the monocarbon compound which is inexhaustible in the nature into mycoprotein, plant nutrition and organic matters, the metabolite of the bacterium has rich diversity and probiotic characteristics, and the probiotic bacterium has the advantages of multi-stress resistance to external environmental factors, no pollution, no toxicity, and the like, and is increasingly applied to the fields of agriculture, medicine, environmental protection and the like in recent years. Therefore, the intensive research and development of the Bacillus methylotrophicus LW-6 has epoch-making practical significance.
In addition, active substances such as antibiotics, polymyxin, nystatin, gramicidin and the like generated by the LW-6 thallus in the growth process of the Bacillus methylotrophicus have obvious inhibition effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of an animal farm. The bacteria liquid is prepared under specific activation and fermentation conditions, and the prepared deodorizing and epidemic preventing agent contains bacillus methylotrophicus LW-6 with high viable count, strong stress resistance and stable effect in the using process of livestock and poultry farms.
In order to achieve the purpose, the invention adopts the technical scheme that:
a microorganism deodorization epidemic prevention agent prepared from Bacillus methylotrophicus LW-6 bacterial liquid and used for air of livestock and poultry farms is prepared by the following method:
(1) inoculating a test tube slant of the bacillus methylotrophicus to a flat plate for colony activation and amplification, and inoculating the amplified colony flat plate to a liquid culture medium for activation culture for 92 hours to prepare activated flora liquid;
(2) inoculating the activated flora liquid prepared in the step (1) into a liquid fermentation culture medium according to the volume percentage of 8%, and performing fermentation culture at 32 ℃ for 120 hours to obtain the bacillus methylotrophicus fermented liquid, wherein the total amount of live spores of the bacillus methylotrophicus fermented liquid reaches more than 100 hundred million/ml. And (3) preparing the bacillus methylotrophicus LW-6 fermented bacterium liquid prepared in the step (2) into the bacillus methylotrophicus LW-6 deodorizing and epidemic preventing agent with the bacterium content of more than or equal to 100 hundred million/ml under the conditions of ultrafiltration concentration and multi-layer filtration.
Preferably, the deodorizing and epidemic preventing agent for Bacillus methylotrophicus LW-6 in the step (1) is characterized in that the Bacillus methylotrophicus LW-6(Bacillus methylotrophicus) in the step (1) has a preservation unit of: CGMCC-China general microbiological culture Collection center; address: the institute of microbiology, national academy of sciences, university in the Chaoyang area, Beijing; the preservation number is: CGMCC NO. 6793; storage time: 11/07/2012, and survival was detected.
According to the invention, the bacillus methylotrophicus deodorizing and epidemic preventing agent in the step (1) is characterized in that the liquid culture medium in the step (1) comprises the following components in percentage by weight: glucose 0.5%, yeast extract powder 0.9%, peptone 0.9%, sodium chloride 1.1%, pH 7.2. According to the present invention, the activation culture conditions in step (1) are preferably bacillus methylotrophicus deodorant and epidemic preventing agents, and the activation culture conditions in step (1) are: inoculating the strain preserved on the inclined plane into a liquid culture medium, and carrying out shaking culture at 32 ℃ and 185 rpm for 92 hours to obtain the activated thallus.
According to the invention, the bacillus methylotrophicus deodorizing and epidemic preventing agent in the step (2) is characterized in that the liquid fermentation medium in the step (2) comprises the following components in percentage by weight: 1.2 percent of glucose, 0.9 percent of sodium chloride, 0.4 percent of ammonium carbonate, 1.5 percent of peptone, 1.3 percent of yeast extract powder, 0.5 percent of monopotassium phosphate and the balance of water, and the pH value is 7.2.
The microbial air deodorization epidemic prevention agent for the methylotrophic bacillus livestock and poultry farm is applied to air treatment of the livestock and poultry farm.
The application comprises the following steps:
the application comprises the following steps:
uniformly mixing the bacillus methylotrophicus deodorizing and epidemic preventing agent with water according to the mass ratio of 1:80, and spraying the mixture into a livestock and poultry breeding shed according to the spraying amount of 15 milliliters per square meter, wherein the spraying frequency is 3 times.
Advantageous effects
1. The bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of livestock farms is applied to livestock farms as a liquid spray for the first time, has low nutritional requirement, strong stress resistance and fast growth and reproduction, can grow and fix plants quickly after being sprayed to the environment, and metabolizes to generate various antibacterial substances, various enzymes and the like, so that the bacterium can be antagonized, inhibited or killed, and the aim of air treatment in the breeding environment of disinfection, deodorization, purification and epidemic prevention is fulfilled.
2. The bacillus methylotrophicus LW-6 is activated and fermented under specific conditions, and is subjected to specific ultrafiltration concentration and multilayer filtration to prepare a liquid spray with the spore number of more than 100 hundred million/ml, so that the microbial activity is stable; solves the problems that the LW-6 thalli of the Bacillus methylotrophicus easily loses activity in transportation, storage and use and the effect is reduced, and has convenient use and ideal deodorization and epidemic prevention effects.
3. The bacillus methylotrophicus LW-6 microbial air deodorization epidemic prevention agent is applied to livestock and poultry farms as a liquid spray for the first time, and has the advantages of low nutritional requirement, strong stress resistance, fast growth and reproduction, fast growth and field planting after being sprayed to the environment, and metabolism to generate various antibacterial substances, various enzymes and the like, so that antagonism, inhibition or killing effects on pathogenic bacteria are generated, and the aim of air treatment in the breeding environment of disinfection, deodorization, purification and epidemic prevention is fulfilled.
Detailed Description
The technical solution of the present invention is further described with reference to the following examples, but the scope of the present invention is not limited thereto.
The bacillus methylotrophicus accession number in example 1 is: CGMCC No.6793, the preservation organization is: bacillus methylotrophicus of the general microbiological center of China Committee for culture Collection of microorganisms.
Example 1:
1 inoculating a test tube slant of the bacillus methylotrophicus to a plate of a specific culture medium for colony activation and amplification, and inoculating the amplified colony plate to a specific liquid culture medium for activation culture for 92 hours to prepare an activated flora liquid;
2 inoculating the activated flora liquid with the volume percentage of 8 percent into a liquid fermentation culture medium with a specific formula, and performing fermentation culture for 120 hours at the temperature of 32 ℃ to obtain the bacillus methylotrophicus.
And 3, carrying out ultrafiltration concentration and multilayer filtration on the bacillus methylotrophicus fermented bacteria liquid under specific conditions to prepare the bacillus methylotrophicus deodorant and epidemic prevention agent with the bacteria content of more than or equal to 100 hundred million/ml.
Wherein, the liquid culture medium in the step 1 comprises the following components in percentage by weight:
glucose 0.5%, yeast extract powder 0.9%, peptone 0.9%, sodium chloride 1.1%, pH 7.2.
The activated culture conditions in step 1 are: inoculating the strain preserved on the inclined plane into a liquid culture medium, and carrying out shaking culture at 185 rpm for 92 hours at the temperature of 32 ℃, thus obtaining the activated thallus.
The liquid fermentation medium in the step 2 comprises the following components in percentage by weight: 1.2 percent of glucose, 0.9 percent of sodium chloride, 0.4 percent of ammonium carbonate, 1.5 percent of peptone, 1.3 percent of yeast extract powder, 0.5 percent of monopotassium phosphate and the balance of water, and the pH value is 7.2.
Example 2:
the bacillus methylotrophicus LW-6 deodorization epidemic prevention agent prepared in the embodiment 1 is uniformly mixed with water according to the mass ratio of 1:80, and is sprayed into a livestock and poultry breeding shed according to the spraying amount of 15 milliliters per square meter for 3 times.
Before chicks enter the shed, 5 plates of different bacteria are placed at different positions (four corners and middle positions) in the shed respectively, the chicken are covered after 10 minutes, the chicken are taken out, then a deodorization and epidemic prevention agent is sprayed in the shed, the chicken are placed into different plates in the same way after 8 hours, the chicken are taken out after 10 minutes, the chicken are placed into a constant-temperature incubator at 32 ℃ for culture for 48 hours, and the number of bacteria before and after disinfection is counted respectively. Calculating the bacteriostasis rate, and obtaining the following results: 72% of staphylococcus aureus, 65% of chicken colibacillus, 82% of salmonella pullorum and 63% of poultry pasteurella multocida.
Example 3:
this example was conducted to examine the deodorizing ability of Bacillus methylotrophicus LW-6 deodorizing and epidemic preventing agent.
The bacillus methylotrophicus LW-6 deodorization epidemic prevention agent prepared in the embodiments 1 and 2 is uniformly mixed with water according to the mass ratio of 1:80, and is sprayed into a livestock and poultry breeding shed according to the spraying amount of 15 milliliters per square meter, chicken manure is randomly taken from a farm, 20 kilograms of the chicken manure is taken respectively, the chicken manure is placed into three test boxes, the bacillus methylotrophicus deodorization epidemic prevention agent is sprayed every 12 hours, and the concentrations of ammonia, methyl mercaptan and hydrogen sulfide are detected after 48 hours.
The results of the bacillus methylotrophicus deodorizing and epidemic preventing agent of the embodiment are shown in the following table:
Claims (6)
1. a bacillus methylotrophicus LW-6 deodorization epidemic prevention agent for air treatment of livestock farms is characterized by being prepared by the following method:
(1) inoculating a test tube slant of the bacillus methylotrophicus to a plate of a specific culture medium for colony activation and amplification, and inoculating the amplified colony plate to a specific liquid culture medium for activation culture (92 hours) to prepare an activated flora liquid;
(2) inoculating the activated flora liquid prepared in the step (1) into a liquid fermentation culture medium with a specific formula according to the volume percentage of 8%, and performing fermentation culture for 120 hours at the temperature of 32 ℃ to prepare the bacillus methylotrophicus fermented liquid.
(3) And (3) carrying out ultrafiltration concentration and multilayer filtration on the bacillus methylotrophicus fermented liquid prepared in the step (2) under specific conditions to prepare the bacillus methylotrophicus deodorant and epidemic prevention agent with the bacterium content of more than or equal to 100 hundred million/ml.
2. The bacillus methylotrophicus LW-6 deodorizing and epidemic preventing agent according to claim 1, wherein the bacillus methylotrophicus LW-6 preservation registration number in step (1) is cgmccno.6793, and the preservation organization is: bacillus methylotrophicus of the general microbiological center of China Committee for culture Collection of microorganisms.
3. The bacillus methylotrophicus deodorant and epidemic preventing agent according to claim 1, wherein the liquid medium in step (1) comprises the following components by weight percent:
glucose 0.5%, yeast extract powder 0.9%, peptone 0.9%, sodium chloride 1.1%, pH 7.2.
4. The bacillus methylotrophicus LW-6 deodorizing and epidemic preventing agent according to claim 1, wherein the activation culture conditions in step (1) are: inoculating the strain preserved on the inclined plane into a liquid culture medium, and carrying out shaking culture at 185 rpm for 92 hours at the temperature of 32 ℃, thus obtaining the activated thallus.
5. The bacillus methylotrophicus LW-6 deodorizing and epidemic preventing agent according to claim 1, wherein the liquid fermentation medium in step (2) comprises the following components, all by weight:
1.2 percent of glucose, 0.9 percent of sodium chloride, 0.4 percent of ammonium carbonate, 1.5 percent of peptone, 1.3 percent of yeast extract powder, 0.5 percent of monopotassium phosphate and the balance of water, and the pH value is 7.2.
6. Use according to claim 1, characterized by the following steps:
uniformly mixing the bacillus methylotrophicus deodorizing epidemic prevention agent with water according to the mass ratio of 1:80, and spraying the mixture into a livestock and poultry breeding shed according to the spraying amount of 15 milliliters per square meter, wherein the spraying frequency is 3 times.
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