CN111662834B - Biological deodorant and preparation method thereof - Google Patents
Biological deodorant and preparation method thereof Download PDFInfo
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- CN111662834B CN111662834B CN202010545708.0A CN202010545708A CN111662834B CN 111662834 B CN111662834 B CN 111662834B CN 202010545708 A CN202010545708 A CN 202010545708A CN 111662834 B CN111662834 B CN 111662834B
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- liquid
- spore
- yeast
- culture
- lactic acid
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- 239000002781 deodorant agent Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 129
- 241000894006 Bacteria Species 0.000 claims abstract description 70
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 64
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 36
- 102000004190 Enzymes Human genes 0.000 claims abstract description 35
- 108090000790 Enzymes Proteins 0.000 claims abstract description 35
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 34
- 239000004310 lactic acid Substances 0.000 claims abstract description 32
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 230000000694 effects Effects 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims description 39
- 238000000855 fermentation Methods 0.000 claims description 36
- 230000004151 fermentation Effects 0.000 claims description 36
- 239000000243 solution Substances 0.000 claims description 16
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 15
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 15
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 15
- 239000002244 precipitate Substances 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 229940041514 candida albicans extract Drugs 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 10
- 238000003825 pressing Methods 0.000 claims description 10
- 238000009423 ventilation Methods 0.000 claims description 10
- 239000012138 yeast extract Substances 0.000 claims description 10
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 8
- 239000011550 stock solution Substances 0.000 claims description 8
- 238000005516 engineering process Methods 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 6
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000007836 KH2PO4 Substances 0.000 claims description 5
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 5
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 238000011033 desalting Methods 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 5
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000013028 medium composition Substances 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 5
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims description 5
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 210000005253 yeast cell Anatomy 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000004332 deodorization Methods 0.000 abstract description 14
- 244000005700 microbiome Species 0.000 abstract description 11
- 239000000419 plant extract Substances 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 3
- 238000000354 decomposition reaction Methods 0.000 abstract description 3
- 239000003344 environmental pollutant Substances 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 231100000719 pollutant Toxicity 0.000 abstract description 3
- 239000003963 antioxidant agent Substances 0.000 abstract description 2
- 239000001569 carbon dioxide Substances 0.000 abstract description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract description 2
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 abstract description 2
- 231100000956 nontoxicity Toxicity 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 230000003078 antioxidant effect Effects 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 12
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 12
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 12
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 12
- 238000012360 testing method Methods 0.000 description 9
- 239000007789 gas Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 229910021529 ammonia Inorganic materials 0.000 description 5
- 239000010865 sewage Substances 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 230000001877 deodorizing effect Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000255925 Diptera Species 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000000686 essence Substances 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000005541 medical transmission Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000009972 noncorrosive effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/72—Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
Abstract
The invention discloses a biological deodorant and a preparation method thereof, belonging to the technical field of environmental protection, wherein the biological deodorant is formed by mixing yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid according to the mass ratio of (1-1.5): 0.05-0.07): 0.001-0.005, and plant extract can be added according to needs, after the biological deodorant is used, antioxidant is generated in a short time, the survival and the propagation of putrefying bacteria are resisted, a biological decomposition layer is formed on the surface of dirt, and gaseous NH is enabled to be generated, and the preparation method can prevent the growth of putrefying bacteria and the propagation of putrefying bacteria3The microorganism can decompose harmful components without escaping, thereby achieving the effect of quick-acting deodorization; after the pollutants are eaten, the microorganisms are decomposed into carbon dioxide, water and nontoxic cell residues, secondary pollution is avoided, pungent smell is not generated in the using process, the microorganisms can be contacted with the skin of a human body or an animal, no toxicity is caused, and special cleaning is not needed.
Description
Technical Field
The invention relates to the technical field of environmental protection, and particularly relates to a biological deodorant and a preparation method thereof.
Background
The urban water body is an important component in urban living environment, but due to the characteristics of easy pollution, small water environment capacity, poor water body self-purification capacity and the like, the urban water body can easily become a receptor of domestic sewage, rainwater and garbage of residents, thereby forming the urban black and odorous water body. In the process that domestic sewage is generated to reach a sewage treatment facility for treatment, the processes of collection, standing, temporary storage and transportation are often needed, once the time is long, the temperature is increased, malodorous gas which takes ammonia gas and hydrogen sulfide as main components is easily generated, and adverse effects are generated on the environment.
In recent years, microorganisms and preparation products thereof are more and more widely applied in the fields of planting, breeding, environmental protection, medical treatment and the like. For example, the patent application of Beijing Prun ecological technology Limited, "a preparation method of high concentration biological deodorant" (patent application publication No. CN 101711885A; application No. 200910250028.X) discloses a process comprising: adding a strain with a deodorizing function into a natural plant with the deodorizing function and extracts thereof, a carbon source, a nitrogen source, trace elements and the like, mixing and fermenting to obtain a high-concentration deodorant containing plant active ingredients, beneficial microorganisms and metabolites, diluting the deodorant by 2000 times with clear water, and directly spraying the deodorant. By decomposing odor substances such as ammonia nitrogen, hydrogen sulfide and the like, the growth of harmful microorganisms generating odor in garbage and odor sources is inhibited, and the aims of deodorizing, repelling mosquitoes and flies and reducing the risk of disease transmission are achieved. The disadvantages of this patent application are: because the plant extract and the microbial strains need to be mixed and fermented in the preparation process, the process is complex, the equipment is expensive, the preparation period is long, the price of the produced biological deodorant is higher, and meanwhile, the existing biological deodorant has longer deodorization time which generally needs about 60 hours; to NH3And H2The removal rate of S is low, and is about 80% and 70% respectively.
Disclosure of Invention
In order to solve the technical problems, the invention provides the biological deodorant with simple preparation process, short preparation period, low price and obvious deodorization effect and the preparation method thereof, which can improve the deodorization effect on NH while shortening the deodorization time3And H2The removal rate of S.
The invention provides a biological deodorant, which comprises the following raw materials: yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid.
Further, the mass ratio of yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid is (1-1.5): 0.05-0.07): 0.001-0.005.
Further, the mass ratio of the yeast liquid to the spore liquid to the lactic acid bacteria liquid to the photosynthetic bacteria liquid to the active enzyme liquid is 1:1:1:0.05: 0.001.
Further, a plant extract may be added to the biological deodorant.
Further, the fermentation conditions of the yeast liquid and the spore liquid are as follows: adopting a ventilation stirring fermentation tank; temperature and time of medium sterilization: 30min at 121 ℃; the culture conditions were: the ventilation volume is 0.1-0.5vvm, the stirring speed is 150-; the yeast adopts YPD culture medium with pH of 5-6; the spore culture is LB culture medium, pH 7-8. The number of yeast cells in the prepared yeast liquid is not less than 104The number of spore bacteria cells in the prepared spore bacteria liquid is not less than 105/mL。
Further, the fermentation conditions of the photosynthetic bacteria liquid are as follows: an anaerobic sealed fermentation tank is adopted, a light source is arranged in the fermentation tank, the illumination intensity is 3000lux, intermittent stirring is carried out, the stirring speed is 30r/min, stirring is carried out for 5min every time, stirring is carried out once every 12h, the culture temperature is 20-30 ℃, and the culture time is 6 d; the medium composition was as follows: NH (NH)4Cl 1.0g，CH3COONa3.5g，MgCl20.1g，CaCl2 0.1g，KH2PO4 0.6g，K2HPO40.4g, 0.1g of yeast extract and 1000mL of distilled water, and the pH value is 7.2. The photosynthetic bacteria in the prepared photosynthetic bacteria liquid has a photosynthetic bacteria count of not less than 104/mL。
Further, the preparation method of the lactic acid bacteria liquid comprises the following steps: intermittently stirring in an anaerobic sealed fermentation tank at a stirring speed of 30r/min for 5min every 12h at a culture temperature of 35-45 ℃ for 3-5 d; the culture medium comprises 10g of yeast extract, 10g of peptone, 10g of beef extract, 10g of glucose, 10g of maltose and 5g of sodium acetate2g of monopotassium phosphate, 2g of ammonium citrate, 0.5g of magnesium sulfate, 0.5g of manganese sulfate and 1000mL of distilled water with the pH value of 4-6. The number of lactobacillus in the prepared lactobacillus liquid is not less than 107/mL。
Further, the preparation method of the active enzyme solution is as follows: separating by ammonium sulfate fractional precipitation method, adding 30% saturation ammonium sulfate into spore fermentation liquid which is fermented and cultured for 36h, standing for 12h at 10 ℃, removing precipitate by plate filter pressing, taking supernatant, then adding ammonium sulfate until the saturation is 75%, standing for 24h at 10 ℃, collecting precipitate by plate filter pressing, dissolving the precipitate in 10 times volume of deionized water, desalting by ultrafiltration membrane technology, wherein the membrane pore diameter is 0.01-0.02 μm, obtaining high-concentration stock solution of active enzyme, and freezing and storing at-20 ℃ for later use. The activity of the active enzyme solution is calculated as 300 mu g/mL by the activity of the tyrosine protease.
The invention also provides a preparation method of the biological deodorant, which comprises the following steps: and mixing the prepared yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid according to a mass ratio in an aseptic operation, and then subpackaging.
The plant extract is commercially available plant food-grade essence, and the plant extract is added with essences with different fragrance types according to the requirements of customers, and the addition ratio is that of the biological deodorant: 10000-100000 of plant extract: 1.
compared with the prior art, the invention has the following beneficial effects:
the preparation process of the biological deodorant is simple, a mixed fermentation process is not needed, the preparation success rate is high, the time is short, the equipment is simple, and the preparation material cost is low.
The biological deodorant prepared by the invention can inhibit microorganisms generating odor by phagocytizing odor substances; accelerating the decomposition speed of organic matters, fundamentally eliminating odor, inhibiting the breeding of harmful bacteria, having rapid action and obvious effect. The biological deodorant prepared by the invention is a pure biological preparation, and is a green and environment-friendly product which is non-toxic, harmless, non-irritant and non-corrosive. Has no harm to human body and animals and plants, eliminates the harm to human body caused by covering odor with chemical flavoring agent, and provides a green, safe and environment-friendly deodorant.
After the biological deodorant of the invention is sprayed in sewage or dirt, microorganisms multiply and grow exponentially to generate antioxidants such as NH in a short time3、H2S、NO2Etc. to inhibit the survival and propagation of decay bacteria, and to form a biological decomposition layer on the surface of the dirt, so that gaseous NH is generated3Is not released, wherein the microorganisms are also able to decompose NH3、H2S, methyl mercaptan and other harmful components to achieve the effect of quick-acting deodorization; after the pollutants are eaten, the microorganisms are decomposed into carbon dioxide, water and nontoxic cell residues, secondary pollution is avoided, pungent smell is not generated in the using process, the microorganisms can be contacted with the skin of a human body or an animal, no toxicity is caused, and special cleaning is not needed.
The removal rate of the biological deodorant to ammonia nitrogen can reach 99 percent at most, and H2The highest removal rate of S can reach 97%, the degradation rate of the odor concentration can reach 98%, the germ inhibition rate of a farm can reach 98%, the degradation rate of the garbage odor concentration can reach 97%, the COD and ammonia nitrogen content in sewage can be obviously reduced, and odor substances in a sewer toilet can be effectively degraded; after long-term use of the biological deodorant disclosed by the invention, a microbial membrane can be formed on pollutants such as the ground, objects, water channel equipment and the like, so that a continuous and stable deodorization effect is achieved.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention. It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
For numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
Example 1
The biological deodorant in the embodiment is yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid, and the mass ratio of the yeast liquid, the spore liquid, the lactic acid bacteria liquid, the photosynthetic bacteria liquid and the active enzyme liquid is 1.5:1.5:1.5:0.07: 0.005.
The fermentation conditions of the yeast liquid and the spore liquid are as follows: adopting a ventilation stirring fermentation tank; temperature and time of medium sterilization: 30min at 121 ℃; the culture conditions were: the ventilation rate is 0.1vvm, the stirring speed is 150r/min, the culture temperature is 30 ℃, and the culture time is 24 h; the yeast adopts YPD culture medium, pH 5; the spore culture is LB culture medium, pH7. The number of yeast cells in the yeast liquid prepared in this example was 8X 104mL, the number of spore cells in the prepared spore suspension was 5X 105/mL。
The fermentation conditions of the photosynthetic bacterial liquid are as follows: an anaerobic sealed fermentation tank is adopted, a light source is arranged in the fermentation tank, the illumination intensity is 3000lux, intermittent stirring is carried out, the stirring speed is 30r/min, stirring is carried out for 5min every time, stirring is carried out once every 12h, the culture temperature is 20 ℃, and the culture time is 6 d; the medium composition was as follows: NH (NH)4Cl 1.0g，CH3COONa 3.5g，MgCl20.1g，CaCl2 0.1g，KH2PO4 0.6g，K2HPO40.4g, 0.1g of yeast extract, 1000mL of distilled water, pH7.2, and the photosynthetic bacteria number in the photosynthetic bacteria solution prepared in this example is 5X 104/mL。
The preparation method of the lactic acid bacteria liquid comprises the following steps: intermittently stirring in an anaerobic sealed fermentation tank at a stirring speed of 30r/min for 5min every 12h at a culture temperature of 35 ℃ for 3 d; the culture medium comprises yeast extract 10g and peptone 10g, 10g of beef extract, 10g of glucose, 10g of maltose, 5g of sodium acetate, 2g of monopotassium phosphate, 2g of ammonium citrate, 0.5g of magnesium sulfate, 0.5g of manganese sulfate, 1000mL of distilled water and pH4, wherein the number of lactic acid bacteria in the lactic acid bacteria liquid prepared in the embodiment is 3 multiplied by 107/mL。
The preparation method of the active enzyme solution comprises the following steps: the method comprises the steps of adopting an ammonium sulfate fractional precipitation method for separation, adding 30% of ammonium sulfate into spore fermentation liquor which is fermented and cultured for 36 hours, standing for 12 hours at 10 ℃, adopting a plate filter pressing method, removing precipitate, taking supernate, then adding the ammonium sulfate until the saturation is 75%, standing for 24 hours at 10 ℃, collecting precipitate through the plate filter pressing method, dissolving the precipitate into 10 times of volume of deionized water, desalting by adopting an ultrafiltration membrane technology, wherein the membrane aperture is 0.01 mu m, obtaining high-concentration stock solution of active enzyme, and freezing and storing the stock solution at-20 ℃ for later use, wherein the activity of the active enzyme solution in the embodiment is calculated as 300 mu g/mL by the activity of tyrosine protease.
The preparation method comprises the steps of mixing the prepared yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid according to the mass ratio in an aseptic operation mode, and then subpackaging.
Example 2
The biological deodorant is yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid, and the mass ratio of the yeast liquid, the spore liquid, the lactic acid bacteria liquid, the photosynthetic bacteria liquid and the active enzyme liquid is 1.2:1.2:1.2:0.06: 0.003.
The fermentation conditions of the yeast liquid and the spore liquid are as follows: adopting a ventilation stirring fermentation tank; temperature and time of medium sterilization: 30min at 121 ℃; the culture conditions were: the ventilation rate is 0.3vvm, the stirring speed is 180r/min, the culture temperature is 33 ℃, and the culture time is 36 h; the yeast adopts YPD culture medium, pH 6; the spore culture is LB culture medium, pH 8. The number of yeast cells in the yeast liquid prepared in this example was 7.5X 104mL, the number of spore cells in the prepared spore suspension was 5X 105/mL。
The fermentation conditions of the photosynthetic bacterial liquid are as follows: adopting an anaerobic sealed fermentation tank, arranging a light source in the fermentation tank, wherein the illumination intensity is 3000lux, intermittently stirring the mixture, and the stirring speed is 30r/minStirring for 5min every time, and stirring once every 12h, wherein the culture temperature is 25 ℃, and the culture time is 6 d; the medium composition was as follows: NH (NH)4Cl 1.0g，CH3COONa 3.5g，MgCl20.1g，CaCl2 0.1g，KH2PO4 0.6g，K2HPO40.4g, 0.1g of yeast extract, 1000mL of distilled water, pH7.2, and the photosynthetic bacteria number in the photosynthetic bacteria solution prepared in this example is 5X 104/mL。
The preparation method of the lactic acid bacteria liquid comprises the following steps: intermittently stirring in an anaerobic sealed fermentation tank at a stirring speed of 30r/min for 5min every 12h at a culture temperature of 40 ℃ for 4 d; the culture medium comprises 10g of yeast extract, 10g of peptone, 10g of beef extract, 10g of glucose, 10g of maltose, 5g of sodium acetate, 2g of monopotassium phosphate, 2g of ammonium citrate, 0.5g of magnesium sulfate, 0.5g of manganese sulfate, 1000mL of distilled water and pH6, and the number of lactic acid bacteria in the lactic acid bacteria liquid prepared in the embodiment is 3 multiplied by 107/mL。
The preparation method of the active enzyme solution comprises the following steps: the method comprises the steps of adopting an ammonium sulfate fractional precipitation method for separation, adding 30% of ammonium sulfate into spore fermentation liquor which is fermented and cultured for 36 hours, standing for 12 hours at 10 ℃, adopting a plate filter pressing method, removing precipitate, taking supernate, then adding the ammonium sulfate until the saturation is 75%, standing for 24 hours at 10 ℃, collecting precipitate through the plate filter pressing method, dissolving the precipitate into 10 times of volume of deionized water, desalting by adopting an ultrafiltration membrane technology, wherein the membrane aperture is 0.02 mu m, obtaining high-concentration stock solution of active enzyme, and freezing and storing the stock solution at-20 ℃ for later use, wherein the activity of the active enzyme solution in the embodiment is calculated as 300 mu g/mL by the activity of tyrosine protease.
The preparation method comprises the steps of mixing the prepared yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid according to the mass ratio in an aseptic operation mode, and then subpackaging.
Example 3
The biological deodorant is yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid, and the mass ratio of the yeast liquid, the spore liquid, the lactic acid bacteria liquid, the photosynthetic bacteria liquid and the active enzyme liquid is 1:1:1:0.05: 0.001.
The fermentation conditions of the yeast liquid and the spore liquid are as follows: adopting a ventilation stirring fermentation tank; temperature and time of medium sterilization: 30min at 121 ℃; the culture conditions were: the ventilation rate is 0.5vvm, the stirring speed is 200r/min, the culture temperature is 37 ℃, and the culture time is 48 h; the yeast adopts YPD culture medium, and pH5.5; the spore culture is LB culture medium, pH7.5. The number of yeast cells in the yeast liquid prepared in this example was 8X 104mL, the number of spore cells in the prepared spore suspension was 5X 105/mL。
The fermentation conditions of the photosynthetic bacterial liquid are as follows: an anaerobic sealed fermentation tank is adopted, a light source is arranged in the fermentation tank, the illumination intensity is 3000lux, intermittent stirring is carried out, the stirring speed is 30r/min, stirring is carried out for 5min every time, stirring is carried out once every 12h, the culture temperature is 30 ℃, and the culture time is 6 d; the medium composition was as follows: NH (NH)4Cl 1.0g，CH3COONa 3.5g，MgCl20.1g，CaCl2 0.1g，KH2PO4 0.6g，K2HPO40.4g, 0.1g of yeast extract, 1000mL of distilled water, pH7.2, and the photosynthetic bacteria number in the photosynthetic bacteria solution prepared in this example is 5X 104/mL。
The preparation method of the lactic acid bacteria liquid comprises the following steps: intermittently stirring in an anaerobic sealed fermentation tank at a stirring speed of 30r/min for 5min every 12h at a culture temperature of 45 ℃ for 5 d; the culture medium comprises 10g of yeast extract, 10g of peptone, 10g of beef extract, 10g of glucose, 10g of maltose, 5g of sodium acetate, 2g of monopotassium phosphate, 2g of ammonium citrate, 0.5g of magnesium sulfate, 0.5g of manganese sulfate, 1000mL of distilled water and pH4-6, and the number of lactic acid bacteria in the lactic acid bacteria liquid prepared in the embodiment is 3 multiplied by 107/mL。
The preparation method of the active enzyme solution comprises the following steps: the method comprises the steps of adopting an ammonium sulfate fractional precipitation method for separation, adding 30% of ammonium sulfate into spore fermentation liquor which is fermented and cultured for 36 hours, standing for 12 hours at 10 ℃, adopting a plate filter pressing method, removing precipitate, taking supernate, then adding the ammonium sulfate until the saturation is 75%, standing for 24 hours at 10 ℃, collecting precipitate through the plate filter pressing method, dissolving the precipitate into 10 times of volume of deionized water, desalting by adopting an ultrafiltration membrane technology, wherein the membrane aperture is 0.01 mu m, obtaining high-concentration stock solution of active enzyme, and freezing and storing the stock solution at-20 ℃ for later use, wherein the activity of the active enzyme solution in the embodiment is calculated as 300 mu g/mL by the activity of tyrosine protease.
The preparation method comprises the steps of mixing the prepared yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid according to the mass ratio in an aseptic operation mode, and then subpackaging.
Example 4
The difference from example 3 is that a plant extract, which is obtained from shanghai yue environmental protection science and technology limited, is added to the biological deodorant in an amount of: 10000 of plant extract: 1 (mass ratio).
Comparative example 1
The difference from example 3 is only that no active enzyme solution was added.
Comparative example 2
The difference from example 3 is only that no photosynthetic bacteria liquid was added.
Comparative example 3
The difference from example 3 is only that the mass ratio of the yeast liquid, the spore liquid, the lactic acid bacteria liquid, the photosynthetic bacteria liquid and the active enzyme liquid is 0.05:0.05:0.05:1: 1.
Comparative example 4
The difference from example 3 is only that the mass ratio of the yeast liquid, the spore liquid, the lactic acid bacteria liquid, the photosynthetic bacteria liquid and the active enzyme liquid is 1:1:1: 1.
The biological deodorant prepared in examples 1 to 4 and comparative examples 1 to 4 and a biological deodorant (as prior art, referred to as comparative example 5) purchased from octovich biotechnology limited in shandong were subjected to deodorization experiments with hydrogen sulfide, ammonia and trimethylamine. When the deodorization test is performed, a solution obtained by diluting a biological deodorant with water 100 times (hereinafter, simply referred to as a biological deodorant aqueous solution) is used unless otherwise specified.
Test example 1 deodorization test of hydrogen sulfide
A3L glass separable flask having a gas inlet for removal, a pressure adjustment port, a measuring port of a measuring tube, and a preliminary port was prepared, and hydrogen sulfide gas was injected from each gas inlet using a syringe and stirred for 10 seconds. Then, the initial concentration of hydrogen sulfide gas was measured by a GASTEC detector tube. Then, after 3mL of the aqueous solution of the biological deodorant was injected, the concentration (mass ppm) of hydrogen sulfide gas after the lapse of time was measured by a GASTEC detector tube, and the deodorization ratio (%) was measured by the following formula (1). The results are shown in Table 1.
TABLE 1
Test example 2 deodorization test of ammonia
The results are shown in Table 2, except that the gas to be measured was changed to ammonia instead of hydrogen sulfide, in the same manner as in test example 1.
TABLE 2
Test example 3 deodorization test of trimethylamine
Except that the measurement target gas was changed to ammonia instead of hydrogen sulfide, the flask was heated to 40 ℃ with a water bath 120 minutes after the trimethylamine was injected, and the deodorization ratio was measured 60 minutes after the start of heating in the same manner as in test example 1, and the results are shown in table 3.
TABLE 3
From tables 1-3, it can be seen that the biological deodorant prepared by the present application has good removal effect on ammonia, amine and hydrogen sulfide. As can be seen from table 3, in the case of using the aqueous solution of a biological deodorant prepared in the example of the present invention, trimethylamine can be completely removed after heating. In the case of using the aqueous solution of a biological deodorant prepared in comparative example 4, the concentration of trimethylamine increased after heating, and it was found that the dissolved trimethylamine was released again.
It should be noted that the above examples are only for illustrating the technical idea and features of the present invention, and the purpose thereof is to enable others to understand the content of the present invention and to implement the present invention, and thus the protection scope of the present invention is not limited thereby. All equivalent changes or improvements made according to the spirit of the invention should be covered within the scope of the invention.
Claims (1)
1. The biological deodorant is characterized by comprising yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid, wherein the mass ratio of the yeast liquid to the spore liquid to the lactic acid bacteria liquid to the photosynthetic bacteria liquid to the active enzyme liquid is 1:1:1:0.05: 0.001;
the fermentation conditions of the yeast liquid and the spore liquid are as follows: adopting a ventilation stirring fermentation tank; temperature and time of medium sterilization: 30min at 121 ℃; the culture conditions were: the ventilation rate is 0.5vvm, the stirring speed is 200r/min, the culture temperature is 37 ℃, and the culture time is 48 h; the yeast adopts YPD culture medium, and pH5.5; the spore culture is LB culture medium, pH7.5, and the yeast cell number in yeast liquid is 8 × 104Perml, the number of spore cells in the spore suspension is 5X 105/mL；
The fermentation conditions of the photosynthetic bacterial liquid are as follows: an anaerobic sealed fermentation tank is adopted, a light source is arranged in the fermentation tank, the illumination intensity is 3000lux, intermittent stirring is carried out, the stirring speed is 30r/min, stirring is carried out for 5min every time, stirring is carried out once every 12h, the culture temperature is 30 ℃, and the culture time is 6 d; the medium composition was as follows: NH (NH)4Cl 1.0g，CH3COONa 3.5g，MgCl20.1g，CaCl2 0.1g，KH2PO4 0.6g，K2HPO40.4g, 0.1g of yeast extract, 1000mL of distilled water, pH7.2, and photosynthetic bacteria number of 5 × 10 in photosynthetic bacteria solution4/mL；
The preparation method of the lactic acid bacteria liquid comprises the following steps: intermittently stirring in an anaerobic sealed fermentation tank at a stirring speed of 30r/min for 5min every 12h at a culture temperature of 45 ℃ for 5 d; the culture medium comprises 10g of yeast extract, 10g of peptone, 10g of beef extract, 10g of glucose, 10g of maltose, 5g of sodium acetate, 2g of monopotassium phosphate, 2g of ammonium citrate, 0.5g of magnesium sulfate, 0.5g of manganese sulfate, 1000mL of distilled water, pH4-6, and the number of lactic acid bacteria in the lactic acid bacteria liquid is 3 multiplied by 107/mL；
The preparation method of the active enzyme solution comprises the following steps: separating by adopting an ammonium sulfate fractional precipitation method, adding 30% of ammonium sulfate into spore fermentation liquor which is fermented and cultured for 36 hours, standing for 12 hours at 10 ℃, adopting a plate filter pressing method, removing precipitate to obtain supernatant, then adding the ammonium sulfate until the saturation is 75%, standing for 24 hours at 10 ℃, collecting precipitate by the plate filter pressing method, dissolving the precipitate into 10 times of volume of deionized water, desalting by adopting an ultrafiltration membrane technology, wherein the membrane aperture is 0.01 mu m to obtain high-concentration stock solution of active enzyme, and freezing and storing at-20 ℃ for later use, wherein the activity of the active enzyme solution is calculated as 300 mu g/mL by the activity of tyrosine protease;
the preparation method of the biological deodorant comprises the steps of mixing the prepared yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid according to a mass ratio in an aseptic operation mode, and then subpackaging.
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