CN107164284A - A kind of deodorizing microorganism microbial inoculum and preparation method thereof - Google Patents

A kind of deodorizing microorganism microbial inoculum and preparation method thereof Download PDF

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Publication number
CN107164284A
CN107164284A CN201710556119.0A CN201710556119A CN107164284A CN 107164284 A CN107164284 A CN 107164284A CN 201710556119 A CN201710556119 A CN 201710556119A CN 107164284 A CN107164284 A CN 107164284A
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China
Prior art keywords
microbial deodorant
microbial
vitamin
bacillus subtilis
preparation
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CN201710556119.0A
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Chinese (zh)
Inventor
李中敏
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Foshan Water Ecology Science And Technology Co Ltd
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Foshan Water Ecology Science And Technology Co Ltd
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Priority to CN201710556119.0A priority Critical patent/CN107164284A/en
Publication of CN107164284A publication Critical patent/CN107164284A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor

Abstract

The invention belongs to field of Environment Protection, a kind of microbial deodorant handled for deodorization is specifically disclosed, the microbial deodorant active component mainly includes microbial bacterial agent, molasses, amino acid and vitamin;The microbial bacteria base is kluyveromyces marxianus and the composite bacteria agent of bacillus subtilis;The microbial deodorant of the present invention can suppress the growth of putrefactivebacteria, improve the decomposition approach of organic matter, reduce burst size and the generation of Ammonia material of ammonia and hydrogen sulfide, while can also reduce bacterium decomposes the methane quantity produced, so as to reach the purpose of deodorization;The microbial deodorant raw material of the present invention is easy to get, preparation technology is simple, is worthy of popularization.

Description

A kind of deodorizing microorganism microbial inoculum and preparation method thereof
Technical field
The invention belongs to field of Environment Protection, and in particular to be a kind of microbial bacterial agent for biological sewage deodorization and its should Be particularly suitable for use in the microbial bacterial agent handled the sanitary sewage of fouling.
Background technology
With developing rapidly for economic society, the continuous acceleration of urbanization process, indoor and outdoor surroundingses pollution is always increasingly dashed forward Go out, the attention of people's environmental pollution goes to interior from outdoor, a major reason for causing pollution is a large amount of of municipal wastewater Discharge, according to statistics, always the company of discharge is about annual 34500000000 tons to China's municipal wastewater, and wherein sanitary sewage accounts for more than 30%, life COD, BOD in sewage5Severe overweight.A kind of foul smell is given out in family bathroom space, drain of kitchen for various reasons, is made People's nausea, dizziness, or even vomiting, directly affects the life of people, jeopardize the health of people.Therefore, the control of malodor source Have become one of environmental problem that urgent need is solved.
Odor pollutant is more, but is generally roughly divided into three classes:The compound of sulfur-bearing(Hydrogen sulfide, methyl mercaptan, Dimethyl sulfide), Nitrogen-containing compound(Ammonia, trimethylamine)And the compound of carbon, hydrogen, oxygen composition(Lower alcohol, aldehyde, aliphatic acid etc.).Wherein H2S and NH3It is The main constituents of stink.
At present, deodorant mainly has a two types, i.e., the absorbent-type deodorant that is prepared using the physical property of material and with Stink chemically react chemistry extract out and, but this two classes deodorant all exist this it is certain the drawbacks of, it is necessary to often change material Material just can guarantee that deodoriging properties, and microbial deodorant can be further enhanced with can constantly be bred in stink assimilation process Deodoriging properties, so as to reach the purpose of long-term deodorization.In recent years, the research work of microbial deodorant, which is concentrated mainly on, efficiently removes The seed selection of smelly dominant strain, using the microorganism formulation of foreign import more than the research of popularization and application, based on the EM bacteria agent of Japan, And the microbial deodorant research conditions of self domestic seed selection and compounding are also rare.
Microbial deodorant is the coefficient result of multiple-microorganism, multiple-microorganism collective effect is more beneficial for absorbing, Decompose the SO produced2、H2S、CH4Deng the pernicious gas of foul smell, meanwhile, multiple-microorganism can produce inorganic acid, and it is unfavorable to be formed The sour environment lived in putrefactive microorganisms, and the material of foul gas is produced when fundamentally degraded is decomposed, this is also EM bacterium One of the reason for applications well effect is obtained in terms of deodorization.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide, a kind of preparation method is simple, cost is low, raw material is easy , deodorizing effect significantly, can fundamentally decompose the microbial deodorant of foul gas.
Another object of the present invention is to provide the application of the microbial deodorant.
Further, the microbial deodorant mainly includes microbial bacterial agent, molasses, amino acid and vitamin;
Further, the microbial bacterial agent is bacillus subtilis, kluyveromyces marxianus;
Further, one or more of the amino acid in glycine, aspartic acid, serine;
Further, the content of honey is 0.3-1 mg/mL, preferably 0.3 mg/mL in the microbial deodorant;
Further, the content of amino acid is 0.3-1mg/mL, preferably 0.6 mg/mL in the microbial deodorant;
Further, one or more of the vitamin in vitamin A, vitamin B12 in the microbial deodorant;
Further, the content of vitamin is 0.6-1mg/mL, preferably 0.6 mg/mL in the microbial deodorant;
The preparation method of the microbial deodorant of the present invention mainly comprises the following steps:
(1)Prepare Marx Shandong dimension yeast fermentation broth;
(2)Prepare bacillus subtilis fermentation liquor;
(3)By step(1)And step(2)The kluyveromyces marxianus zymotic fluid and bacillus subtilis fermentation liquor prepared It is 0.4-1.5 according to volume ratio:1 ratio is sufficiently mixed.
(4)In step(3)In the fermentation liquid mixture kind addition molasses, amino acid and the vitamin that prepare, final system Obtain microbial deodorant of the present invention
In one embodiment of the invention, the preparation method of kluyveromyces marxianus zymotic fluid is:Marx's Crewe is tieed up It is inoculated in after saccharomycete activation in the fluid nutrient medium of culture yeasts bacterium, at 28-35 DEG C, in 120-200 r/min shaking tables 48-72 h are cultivated, the concentration of kluyveromyces marxianus is adjusted to 1 × 10 with sterilized water after counting7-1×109CFU/mL。
In one embodiment of the invention, the preparation method of the bacillus subtilis fermentation liquor is:By withered grass gemma Bacillus is seeded in bacteria culture media, at 25-35 DEG C, and 36-48 h are cultivated in 120-200 r/min shaking tables, by counting The concentration of bacillus subtilis is adjusted to 1 × 10 with sterilized water afterwards7-1×109CFU/mL。
Further, microbial deodorant of the invention can also be used in combination with other microorganisms, and deodorizing effect is more preferably.
Microorganism fungus kind can be commercially available by conventional commercial means in the microbial bacterial agent of the present invention, of the invention The purchase of kluyveromyces marxianus bacterium grinds bio tech ltd from Shanghai one, and bacillus subtilis of the invention is purchased from Xingtai Si Beite bio tech ltd;
Compared with prior art, microbial deodorant of the invention has the beneficial effect that:
1st, microbial deodorant of the invention can suppress the growth of putrefactivebacteria, improve the decomposition approach of organic matter, reduce ammonia Burst size and the generation of Ammonia material with hydrogen sulfide, while can also reduce bacterium decomposes the methane quantity produced, are removed so as to reach Smelly purpose;
2nd, in microbial deodorant of the invention bacillus subtilis and kluyveromyces marxianus each other without antagonism, And both of which can suppress the breeding of pathogenic microbes, be conducive to preventing and treating the generation of disease in sewage;
3rd, microbial deodorant preparation method of the invention is simple, and raw material is easy to get, wide adaptability, continues the deodorization time long.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, specific embodiment party below to the present invention Formula is described in detail.Many details are elaborated in the following description to fully understand the present invention.But this hair Bright to be implemented with being much different from other manner described here, those skilled in the art can be without prejudice in the present invention Similar improvement is done in the case of culvert, therefore the present invention is not limited to the specific embodiments disclosed below.
First, embodiment is prepared
1st, embodiment 1
A kind of microorganism microbial deodorant, is prepared by following preparation method:
(1)Prepare Marx Shandong dimension yeast fermentation broth:Culture yeasts bacterium is inoculated in after kluyveromyces marxianus bacterium is activated In fluid nutrient medium, at 35 DEG C, 72 h are cultivated in 120 r/min shaking tables, Marx is adjusted with sterilized water after counting The concentration of kluyveromyces is to 1 × 109CFU/mL;
(2)Prepare bacillus subtilis fermentation liquor:Bacillus subtilis is seeded in bacteria culture media, at 25 DEG C, 36 h are cultivated in 180 r/min shaking tables, the concentration of bacillus subtilis is adjusted to 1 × 10 with sterilized water after counting8CFU/ mL;
(3)By step(1)And step(2)Zymotic fluid according to 0.8:1 volume ratio mixing;
(4)Liquid mixture kind of fermenting addition molasses, serine, vitamin A, and it is respectively 0.5 mg/mL, 0.3 to adjust its concentration Mg/mL, 0.6 mg/mL, you can microbial deodorant of the present invention is made.
2nd, embodiment 2
A kind of microorganism microbial deodorant, is prepared by following preparation method:
(1)Prepare Marx Shandong dimension yeast fermentation broth:Culture yeasts bacterium is inoculated in after kluyveromyces marxianus bacterium is activated In fluid nutrient medium, at 28 DEG C, 48 h are cultivated in 200 r/min shaking tables, Marx is adjusted with sterilized water after counting The concentration of kluyveromyces is to 1 × 107CFU/mL;
(2)Prepare bacillus subtilis fermentation liquor:Bacillus subtilis is seeded in bacteria culture media, at 35 DEG C, 36 h are cultivated in 200 r/min shaking tables, the concentration of bacillus subtilis is adjusted to 1 × 10 with sterilized water after counting7CFU/ mL;
(3)By step(1)And step(2)Zymotic fluid according to 0.4:1 volume ratio mixing;
(4)Liquid mixture kind of fermenting addition molasses, glycine, aspartic acid, vitamin B12, and it is respectively 0.3 to adjust its concentration Mg/mL, 0.3 mg/mL, 0.3 mg/mL, 0.3 mg/mL, you can microbial deodorant of the present invention is made.
3rd, embodiment 3:
A kind of microorganism microbial deodorant, is prepared by following preparation method:
(1)Prepare Marx Shandong dimension yeast fermentation broth:Culture yeasts bacterium is inoculated in after kluyveromyces marxianus bacterium is activated In fluid nutrient medium, at 28 DEG C, 72h is cultivated in 160 r/min shaking tables, Marx is adjusted with sterilized water after counting The concentration of kluyveromyces is to 1 × 109CFU/mL;
(2)Prepare bacillus subtilis fermentation liquor:Bacillus subtilis is seeded in bacteria culture media, at 30 DEG C, 42 h are cultivated in 160 r/min shaking tables, the concentration of bacillus subtilis is adjusted to 1 × 10 with sterilized water after counting7CFU/ mL;
(3)By step(1)And step(2)Zymotic fluid according to 1.1:1 volume ratio mixing;
(4)Liquid mixture kind of fermenting addition molasses, glycine, aspartic acid, serine, vitamin B12, and adjust its concentration point Wei 0.3 mg/mL, 0.2 mg/mL, 0.2 mg/mL, 0.2mg/mL, 0.3 mg/mL, you can micro- life of the present invention is made Thing deodorant.
4th, comparative example 1
A kind of microbial deodorant, its preparation method is that embodiment 3 removes step(1)And step(3)The microorganism prepared Deodorant.
5th, comparative example 2
A kind of microbial deodorant, its preparation method is that embodiment 3 removes step(2)And step(3)The micro- life prepared Thing deodorant.
2nd, Application Example
Embodiment 1-3 and comparative example 1-2 microbial deodorant are diluted with water after 100 times, rubbish is sprayed at spraying establishing Surface, determines deodorizing effect, and result of the test is as shown in table 1:
Result of the test shows that 1-3 of embodiment of the present invention microbial deodorant is originated to foul smell such as ammonia, hydrogen sulfide and ozone With preferable removal effect, hence it is evident that be better than reference examples 1 and 2;In addition, experiment is it was additionally observed that embodiment 1-3 deodorizing effect exists Sprinkling can also keep stronger deodorizing effect after 7 days, and comparative example 1,2 respectively 4 after spray is swept, 3 days deodorizing effects show It is obvious to decline;Showing microbial deodorant of the invention, not only deodorizing effect is strong, and lasting effect is preferable.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using of the invention special Profit.A variety of modifications to these embodiments will be apparent for those skilled in the art, determine herein The General Principle of justice can in other embodiments be realized in the case where not departing from the spirit or scope of patent of the present invention.Cause This, patent of the present invention is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein The most wide scope consistent with features of novelty.

Claims (10)

1. a kind of microbial deodorant, it is characterised in that the microbial deodorant mainly includes microbial bacterial agent, molasses, ammonia Base acid and vitamin.
2. microbial deodorant according to claim 1, it is characterised in that the microbial bacterial agent is withered grass gemma Bacillus, the combination of kluyveromyces marxianus.
3. microbial deodorant according to claim 1, it is characterised in that the amino acid is selected from glycine, asparagus fern ammonia One or more in acid, serine.
4. microbial deodorant according to claim 1, it is characterised in that the content of honey in the microbial deodorant For 0.3-1 mg/mL, preferably 0.3 mg/mL.
5. microbial deodorant according to claim 1, it is characterised in that amino acid contains in the microbial deodorant Measure as 0.3-1mg/mL, preferably 0.6 mg/mL.
6. microbial deodorant according to claim 1, it is characterised in that vitamin is selected from the microbial deodorant One or more in vitamin A, vitamin B12.
7. microbial deodorant according to claim 1, it is characterised in that vitamin contains in the microbial deodorant Measure as 0.6-1mg/mL, preferably 0.6 mg/mL.
8. the preparation method of microbial deodorant according to claim 1, it is characterised in that comprise the following steps:
(1)Prepare Marx Shandong dimension yeast fermentation broth;
(2)Prepare bacillus subtilis fermentation liquor;
(3)By step(1)And step(2)The kluyveromyces marxianus zymotic fluid and bacillus subtilis fermentation liquor prepared It is 0.4-1.5 according to volume ratio:1 ratio is sufficiently mixed;
(4)In step(3)In the fermentation liquid mixture kind addition molasses, amino acid and the vitamin that prepare, it is final that this is made The described microbial deodorant of invention.
9. preparation method according to claim 8, it is characterised in that the preparation of the kluyveromyces marxianus zymotic fluid Method is:It is inoculated in after kluyveromyces marxianus bacterium is activated in the fluid nutrient medium of culture yeasts bacterium, at 28-35 DEG C, 48-72 h are cultivated in 120-200 r/min shaking tables, the concentration of kluyveromyces marxianus is adjusted with sterilized water after counting To 1 × 107-1×109CFU/mL。
10. preparation method according to claim 8, it is characterised in that the preparation side of the bacillus subtilis fermentation liquor Method is:Bacillus subtilis is seeded in bacteria culture media, at 25-35 DEG C, cultivated in 120-200 r/min shaking tables 36-48 h, the concentration of bacillus subtilis is adjusted to 1 × 10 after counting with sterilized water7-1×109CFU/mL。
CN201710556119.0A 2017-07-10 2017-07-10 A kind of deodorizing microorganism microbial inoculum and preparation method thereof Pending CN107164284A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107488617A (en) * 2017-09-28 2017-12-19 无锡盛雅生物科技有限公司佛山分公司 A kind of complex microbial inoculum for deodorization
CN107570002A (en) * 2017-09-28 2018-01-12 无锡盛雅生物科技有限公司佛山分公司 Composite type deodorization agent for toilet deodorization and preparation method thereof
CN107594515A (en) * 2017-11-08 2018-01-19 山东好当家海洋发展股份有限公司 The preparation method of one main laminaria zymotic fluid
CN108217971A (en) * 2017-12-08 2018-06-29 广州资源环保科技股份有限公司 A kind of microbial deodorant and preparation method thereof
CN109182223A (en) * 2018-10-28 2019-01-11 深圳市爱格丽生物科技有限公司 A kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly
CN113337439A (en) * 2021-06-18 2021-09-03 北京天益源生物科技有限公司 Quick-acting deodorant and preparation method thereof

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CN104689356A (en) * 2013-12-05 2015-06-10 无锡市锡源锅炉有限公司 Method for preparing drying furnace deodorant by composite microbes
CN105585235A (en) * 2016-01-22 2016-05-18 江苏佳净洁环境科技有限公司 Compound microbial preparation with deodorization function, preparation method and application
CN106902631A (en) * 2017-02-23 2017-06-30 浙江加百列生物科技有限公司 A kind of microbial deodorant and production technology

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Publication number Priority date Publication date Assignee Title
JPH06220772A (en) * 1992-05-19 1994-08-09 Takuo Sakai Scouring of fiber
CN101824395A (en) * 2009-03-06 2010-09-08 华东理工大学 Method for culturing fermentation seed liquid by adopting solid straws as carbon source
CN104689356A (en) * 2013-12-05 2015-06-10 无锡市锡源锅炉有限公司 Method for preparing drying furnace deodorant by composite microbes
CN105585235A (en) * 2016-01-22 2016-05-18 江苏佳净洁环境科技有限公司 Compound microbial preparation with deodorization function, preparation method and application
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107488617A (en) * 2017-09-28 2017-12-19 无锡盛雅生物科技有限公司佛山分公司 A kind of complex microbial inoculum for deodorization
CN107570002A (en) * 2017-09-28 2018-01-12 无锡盛雅生物科技有限公司佛山分公司 Composite type deodorization agent for toilet deodorization and preparation method thereof
CN107594515A (en) * 2017-11-08 2018-01-19 山东好当家海洋发展股份有限公司 The preparation method of one main laminaria zymotic fluid
CN107594515B (en) * 2017-11-08 2020-08-04 山东好当家海洋发展股份有限公司 Preparation method of kelp fermentation liquor
CN108217971A (en) * 2017-12-08 2018-06-29 广州资源环保科技股份有限公司 A kind of microbial deodorant and preparation method thereof
CN109182223A (en) * 2018-10-28 2019-01-11 深圳市爱格丽生物科技有限公司 A kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly
CN113337439A (en) * 2021-06-18 2021-09-03 北京天益源生物科技有限公司 Quick-acting deodorant and preparation method thereof

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Application publication date: 20170915