CN107488617A - A kind of complex microbial inoculum for deodorization - Google Patents

A kind of complex microbial inoculum for deodorization Download PDF

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Publication number
CN107488617A
CN107488617A CN201710904118.0A CN201710904118A CN107488617A CN 107488617 A CN107488617 A CN 107488617A CN 201710904118 A CN201710904118 A CN 201710904118A CN 107488617 A CN107488617 A CN 107488617A
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China
Prior art keywords
microbial inoculum
complex microbial
bacterium
yeast strain
deodorization
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CN201710904118.0A
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Chinese (zh)
Inventor
庞婉青
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Wuxi Sheng Ya Biological Science And Technology Co Ltd Foshan Branch
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Wuxi Sheng Ya Biological Science And Technology Co Ltd Foshan Branch
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Priority to CN201710904118.0A priority Critical patent/CN107488617A/en
Publication of CN107488617A publication Critical patent/CN107488617A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/46Removing components of defined structure
    • B01D53/48Sulfur compounds
    • B01D53/52Hydrogen sulfide
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/46Removing components of defined structure
    • B01D53/54Nitrogen compounds
    • B01D53/58Ammonia
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/74General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84Biological processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2257/00Components to be removed
    • B01D2257/90Odorous compounds not provided for in groups B01D2257/00 - B01D2257/708
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

The invention discloses a kind of complex microbial inoculum for deodorization and application, the complex microbial inoculum contains kluyveromyces marxianus and EM bacterium, it is then 0.2 0.5 according to both volume ratios after the complex microbial inoculum can be by the way that kluyveromyces marxianus and EM bacterium be fermented respectively:1 volume ratio mixing, complex microbial inoculum preparation method of the invention is simple, can quickly go to eliminate the unusual smell and eliminate odorant source, and lasting deodorizing effect is excellent, and after spraying 8 days, deodorizing effect remains to maintain higher level.

Description

A kind of complex microbial inoculum for deodorization
Technical field
The invention belongs to environmental protection and microorganism field, and in particular to be a kind of complex microbial inoculum for deodorization and Its preparation method.
Background technology
With the development and the improvement of people's living standards of industrial and agricultural production, pollutant caused by the mankind is continuously increased, special It is not that since the last century, the mankind destroy the hair at full speed for having brought social civilization and science and technology with large-scale environmental protection Exhibition, substantial amounts of soil, water body are by different degrees of pollution so that the benign cycle of ecological environment receives serious influence And destruction, also cause and cause harm to the health of the mankind and self-growth, environmental pollution increasingly becomes being total to for every country With one of problem, environmental pollution has become the significant problem of current international community with protection, right especially in developing country The control of environmental pollution has reached very urgent stage.A major reason for causing pollution is a large amount of of municipal wastewater Discharge, according to statistics, always the company of discharge is about annual 34500000000 tons to China's municipal wastewater, and wherein sanitary sewage accounts for more than 30%, life COD, BOD in sewage5Severe overweight.A kind of foul smell is given out in family bathroom space, drain of kitchen for various reasons, is made People's nausea, dizziness, or even vomiting, directly affects the life of people, jeopardize the health of people.Therefore, the control of malodor source Have become one of environmental problem for being badly in need of solving.
Deodorant mainly includes physical deodorization agent, chemical deodorizing agent, microbial deodorant, plant deodorant and compound removed Smelly dose.Wherein, stink substance is mainly adsorbed onto in absorbent hole by Physical deodorization by adsorbent, during Physical deodorization deodorization Between it is fast, but cost is higher, and adsorbent can not reuse, and the adsorbent containing stink substance is not easily recycled, and is easily caused Secondary pollution.Chemical method mainly mitigates stench, wherein chemical deodorizing agent by using chemical deodorizing material and aromatic substance Use can not thoroughly remove odorant, aromatic can bring unpleasant odour because fragrance scent is too strong.Microbial deodorant Agent mainly utilizes microorganism the odorant source on rubbish surface can be used for microorganism itself, is lived by microbial metabolism It is dynamic to make its degraded, in general, multiple-microorganism collective effect is more beneficial for absorbing, decompose caused by SO2、H2S、CH4Deng stench The pernicious gas of taste, meanwhile, multiple-microorganism can produce inorganic acid, form the acyclic acidic for being unfavorable for putrefactive microorganisms life Border, and fundamentally degrade and the material of foul gas is produced when decomposing, this is also that EM bacterium obtain applications well effect in terms of deodorization One of the reason for fruit.
The foul smell source on rubbish or sanitary sewage surface can be removed to a certain extent using microbial bacterial agent, but using micro- Biological treatment equally exists some problems, for example, deodorizing effect can not continue, so as to result in the need for repeatedly uninterruptedly using micro- life Thing deodorant carries out rubbish deodorization, causes substantial amounts of manpower and materials and wastes, and therefore, there is a kind of deodorization duration length to be supplied Complex microbial inoculum to adapt to the requirement of environmental protection.
The content of the invention
It is an object of the invention to provide a kind of complex microbial inoculum for deodorization, the complex microbial inoculum master There is kluyveromyces marxianus bacterium, the zymotic fluid of EM bacterium mixes, complex microbial inoculum good deodorization effect of the invention, The deodorization duration is grown, it is possible to reduce complex microbial inoculum application times, saves labour.
Another object of the present invention is to provide the application of the complex microbial inoculum, the complex microbial inoculum can It is widely used in the deodorization of rubbish heap, lavatory, kitchen, sanitary sewage surface.
Another object of the present invention is in the preparation method with providing the complex microbial inoculum.
To reach above-mentioned purpose, the present invention adopts the following technical scheme that:
A kind of complex microbial inoculum, it mainly includes yeast strain, the zymotic fluid of EM bacterium mixes.
Wherein, the yeast strain is kluyveromyces marxianus.
The preparation method of the complex microbial inoculum is:
A:The preparation of yeast strain ferments liquid;
B:The preparation of EM bacterial strain fermentation liquors;
C:By yeast strain ferments liquid and EM bacterial strain fermentation liquors using volume ratio as 0.2-0.5:1 volume ratio is well mixed, It can be prepared by complex microbial inoculum of the present invention.
Further, the preparation method of the yeast strain ferments liquid is:The activation of kluyveromyces marxianus bacterium is followed by Kind at 28-35 DEG C, cultivates 48-72h in the fluid nutrient medium of culture yeasts bacterium in 120-200r/min shaking tables, passes through The concentration of kluyveromyces marxianus is adjusted to 1 × 10 with sterilized water after counting7-1×109CFU/mL。
Wherein, the fluid nutrient medium is YPD culture mediums.
Further, the preparation method of the EM zymotic fluids is:0.2-0.5Kg brown sugar is added in warm water, is sufficiently stirred Dissolving, when being cooled to room temperature, 6-10g EM strains are added, are sealed by fermentation 5-8 days at a temperature of 25-35 DEG C, obtain EM fermentations Liquid.
Further, more preferably type of service is microbial fermentation solution to complex microbial inoculum of the invention, can also be led to Cross conventional method and be processed into freeze-dried powder, or other composite bacteria agents are combined into other deodorant.
Further, other deodorant can be chemical deodorizing agent, plant source deodorant etc..
Further, complex microbial inoculum of the invention regards its use state, can be made by way of launching, spraying With.
Microbial strains used herein can be bought by conventional commercial means and be obtained, for example, the present invention is implemented The purchase of kluyveromyces marxianus bacterium grinds bio tech ltd from Shanghai one used in example, and the EM bacterium are revived purchased from Shandong Ke Han bioengineering limited company.
Compared with prior art, deodorizing microorganism composite bacteria agent of the invention has the beneficial effect that:
1st, while complex microbial inoculum of the invention quickly can go to eliminate the unusual smell, odoriferous thing can be thoroughly eliminated, It is capable of the maintenance deodouring effect of long period;
2nd, complex microbial inoculum nonhazardous of the invention effect, has no irritating odor, will not cause secondary pollution;
3rd, complex microbial inoculum of the invention is due to efficiency time length, first use, so that it may prevent and kill off foul smell well Source, be advantageous to save labour;
4th, complex microbial inoculum raw material of the invention is easy to get, and preparation technology is simple, and production cost is relatively low.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, below to the specific reality of the present invention The mode of applying is described in detail.Many details are elaborated in the following description in order to fully understand the present invention.But The invention can be embodied in many other ways as described herein, and those skilled in the art can be without prejudice to this hair Similar improvement is done in the case of bright intension, therefore the present invention is not limited to the specific embodiments disclosed below.
First, the preparation of complex microbial inoculum
1st, embodiment 1
A kind of complex microbial inoculum, it can be prepared by the following method to obtain:
A:The preparation of yeast strain ferments liquid:The liquid of culture yeasts bacterium is inoculated in after kluyveromyces marxianus bacterium is activated In body culture medium, at 28 DEG C, 72h is cultivated in 120r/min shaking tables, Marx's Crewe is adjusted with sterilized water after counting The concentration of yeast is tieed up to 1 × 107CFU/mL。
B:The preparation of EM bacterial strain fermentation liquors:0.2Kg brown sugar is added in warm water, dissolving is sufficiently stirred, is cooled to room temperature When, 10g EM strains are added, is sealed by fermentation 5 days at a temperature of 25 DEG C, obtains EM zymotic fluids.
C:By yeast strain ferments liquid and EM bacterial strain fermentation liquors using volume ratio as 0.2:1 volume ratio is well mixed, you can Complex microbial inoculum of the present invention is made.
2nd, embodiment 2
A kind of complex microbial inoculum, it can be prepared by the following method to obtain:
A:The preparation of yeast strain ferments liquid:The liquid of culture yeasts bacterium is inoculated in after kluyveromyces marxianus bacterium is activated In body culture medium, at 30 DEG C, 48h is cultivated in 180r/min shaking tables, Marx's Crewe is adjusted with sterilized water after counting The concentration of yeast is tieed up to 1 × 109CFU/mL。
B:The preparation of EM bacterial strain fermentation liquors:0.4Kg brown sugar is added in warm water, dissolving is sufficiently stirred, is cooled to room temperature When, 6g EM strains are added, is sealed by fermentation 8 days at a temperature of 25 DEG C, obtains EM zymotic fluids.
C:By yeast strain ferments liquid and EM bacterial strain fermentation liquors using volume ratio as 0.3:1 volume ratio is well mixed, you can Complex microbial inoculum of the present invention is made.
3rd, embodiment 3
A kind of complex microbial inoculum, it can be prepared by the following method to obtain:
A:The preparation of yeast strain ferments liquid:The liquid of culture yeasts bacterium is inoculated in after kluyveromyces marxianus bacterium is activated In body culture medium, at 36 DEG C, 60h is cultivated in 150r/min shaking tables, Marx's Crewe is adjusted with sterilized water after counting The concentration of yeast is tieed up to 1 × 108CFU/mL。
B:The preparation of EM bacterial strain fermentation liquors:0.3Kg brown sugar is added in warm water, dissolving is sufficiently stirred, is cooled to room temperature When, 8g EM strains are added, is sealed by fermentation 6 days at a temperature of 25 DEG C, obtains EM zymotic fluids.
C:By yeast strain ferments liquid and EM bacterial strain fermentation liquors using volume ratio as 0.5:1 volume ratio is well mixed, you can Complex microbial inoculum of the present invention is made.
4th, comparative example 1
A kind of microbial bacterial agent, it is prepared by following preparation method:
It is inoculated in after kluyveromyces marxianus bacterium is activated in the fluid nutrient medium of culture yeasts bacterium, at 36 DEG C, Cultivate 60h in 150r/min shaking tables, after counting with sterilized water adjust the concentration of kluyveromyces marxianus to 1 × 108CFU/mL.5th, comparative example 2
0.3Kg brown sugar is added in warm water, is sufficiently stirred dissolving, when being cooled to room temperature, 8g EM strains are added, 25 It is sealed by fermentation 6 days at a temperature of DEG C, obtains EM zymotic fluids.
2nd, Application Example
Deodorizing effect experiment, test site selection are carried out using 1-3 of the embodiment of the present invention and the microbial bacterial agent of comparative example 3 In Nanhai District Foshan City refuse landfill, selection house refuse is concentrated, humidity is larger, the obvious rubbish heap of foul smell, each It is 10m that rubbish area delimited in processing2, test and carry out at noon, temperature is 25 DEG C, by used 50 times of microbial bacteria dilution agent Rubbish surface is uniformly sprayed at afterwards.
Check refuse odor source NH within 3 days, 8 days after spraying respectively3And H2S, wherein,:H2S detection uses methylene Blue colorimetric method, NH3Test use sodium hypochlorite-salicylic acid AAS, result of the test is as shown in table 1:
The different microorganisms inorganic agent of table 1 compares the clearance and lasting effect of foul smell
Result of the test shows that 1-3 of embodiment of the present invention complex microbial inoculum is respectively provided with well to ammonia and hydrogen sulfide Deodorizing effect, hence it is evident that due to microbial bacterial agent being used alone in comparative example 1,2 to the removal effect of foul smell;It is noticeable It is that the removal continuation of complex microbial inoculum of the invention to foul smell is significantly better than single microbial inoculum, complex microbial inoculum exists Sprinkling can also maintain higher level after 8 days, and the microbial bacterial agent deodorizing effect of comparative example 1,2 occurs for 8 days after spraying It is obvious to decline, show the complex microbial inoculum good deodorization effect of the present invention, be worth the upper popularization and application of production.
Described above, only highly preferred embodiment of the present invention, not makees any formal limitation to the present invention, Any those skilled in the art, without departing from the scope of the technical proposal of the invention, utilize the method for the disclosure above Content makes many possible changes and modifications to technical solution of the present invention, belongs to the scope of claims protection.

Claims (7)

1. a kind of complex microbial inoculum for deodorization, it is characterised in that the composite bacteria agent includes yeast strain, EM bacterium Zymotic fluid mixes.
2. complex microbial inoculum according to claim 1, it is characterised in that the yeast strain is Marx's Crewe Tie up yeast.
3. the preparation method of complex microbial inoculum according to claim 1 or 2, it is characterised in that comprise the following steps:
A:The preparation of yeast strain ferments liquid;
B:The preparation of EM bacterial strain fermentation liquors;
C:By yeast strain ferments liquid and EM bacterial strain fermentation liquors using volume ratio as 0.2-0.5:1 volume ratio is well mixed, you can Complex microbial inoculum of the present invention is made.
4. the preparation method of complex microbial inoculum according to claim 3, it is characterised in that the yeast strain ferments The preparation method of liquid is:It is inoculated in after kluyveromyces marxianus bacterium is activated in the fluid nutrient medium of culture yeasts bacterium, in 28- At 35 DEG C, 48-72h is cultivated in 120-200r/min shaking tables, kluyveromyces marxianus are adjusted with sterilized water after counting Concentration to 1 × 107-1×109CFU/mL。
5. the preparation method of complex microbial inoculum according to claim 3, it is characterised in that the EM bacterial strain fermentation liquors Preparation method be:0.2-0.5Kg brown sugar is added in warm water, is sufficiently stirred dissolving, when being cooled to room temperature, adds 6-10g EM strains, it is sealed by fermentation 5-8 days at a temperature of 25-35 DEG C, obtains EM zymotic fluids.
6. complex microbial inoculum according to claim 1 or 2, it is characterised in that the complex microbial inoculum can be with Other deodorant are used cooperatively.
7. complex microbial inoculum according to claim 6, it is characterised in that the user of the complex microbial inoculum Method is that microbial fermentation solution sprinkling uses or is processed into freeze-dried powder by conventional method.
CN201710904118.0A 2017-09-28 2017-09-28 A kind of complex microbial inoculum for deodorization Pending CN107488617A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS521032A (en) * 1975-06-24 1977-01-06 Tsurumi Soda Kk Deodorant
CN104436262A (en) * 2014-12-09 2015-03-25 镇江拜因诺生物科技有限公司 Microbial air deodorant
CN106244503A (en) * 2016-09-29 2016-12-21 厦门华厦学院 A kind of deodorization EM bacterium solution preparation method
CN107164284A (en) * 2017-07-10 2017-09-15 佛山市水创科联生态科技有限公司 A kind of deodorizing microorganism microbial inoculum and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS521032A (en) * 1975-06-24 1977-01-06 Tsurumi Soda Kk Deodorant
CN104436262A (en) * 2014-12-09 2015-03-25 镇江拜因诺生物科技有限公司 Microbial air deodorant
CN106244503A (en) * 2016-09-29 2016-12-21 厦门华厦学院 A kind of deodorization EM bacterium solution preparation method
CN107164284A (en) * 2017-07-10 2017-09-15 佛山市水创科联生态科技有限公司 A kind of deodorizing microorganism microbial inoculum and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
申友年等: "神奇的EM", 《中学生物教学》 *

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