CN101787350B - Functional composite biological agent for high-efficiency degradation of household refuses and application thereof - Google Patents

Functional composite biological agent for high-efficiency degradation of household refuses and application thereof Download PDF

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Publication number
CN101787350B
CN101787350B CN201010100179XA CN201010100179A CN101787350B CN 101787350 B CN101787350 B CN 101787350B CN 201010100179X A CN201010100179X A CN 201010100179XA CN 201010100179 A CN201010100179 A CN 201010100179A CN 101787350 B CN101787350 B CN 101787350B
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percolate
degradation
bacteria
biological agent
landfill
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CN101787350A (en
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邱忠平
陈家宏
韩云平
周俊强
冯猛
王倩
周丽萍
童霏
廖禧
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Southwest Jiaotong University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a functional composite biological agent for high-efficiency degradation of household refuses and application thereof. The functional composite biological agent consists of high-efficiency cellulose-degrading bacteria and refuse leachate COD degrading bacteria, wherein the volume ratio of the high-efficiency cellulose-degrading bacteria to the refuse leachate COD degrading bacteria is 1-5:1 (V/V); and the microbial population has good synergistic effect. The functional composite biological agent for the high-efficiency degradation of the household refuses has high-efficiency and stable ability to degrade common organic wastes in the refuses, has high adaptability and contamination resistance in different environments, and has broad application prospect in aspects of organic waste treatment and disposal and resource recycling, and the like.

Description

Functional composite biological agent for high-efficiency degradation of household refuses and application thereof
Technical field
The invention belongs to the microbiobacterial agent scope, particularly a kind of structure of functional composite biological agent for high-efficiency degradation of household refuses, making and the application in refuse treatment disposal and resource utilization thereof.
Background technology
Along with human substance life horizontal's raising, the continuous increase of city quantity and scale, domestic waste has become serious social concern.Data shows according to the relevent statistics; China's rubbish average growth rate per annum reaches more than 10%; Become one of the most serious country of garbage-surrounded city in the world, press for of the pollution problem of effective control domestic refuse, need badly comprehensively domestic waste is effectively handled and disposed environment.
The garbage sanitary filling disposal options is since coming out; Reliable owing to possess skills, technology simple, convenient management and reduced investment, working cost are low, applied widely, component of refuse is not had strict demand, can make series of advantages such as ultimate disposal; Obtained using widely in many countries; Account for 70% of whole garbage treatment quantities at China's landfill disposal, and in the long duration from now on, sanitary landfill will be China's domestic rubbish disposal and the irreplaceable main means of disposal.But existing traditional garbage sanitary filling treatment and disposal technology only landfill yard as a passive system that accepts rubbish; The natural degradation speed of landfill waste is very slow, and the percolate difficult treatment still need be carried out maintenance and management to landfill yard in significant period of time after closing; Exist and take a large amount of soils; Safeguard after the closing that the supervision phase is long, landfill yard is used drawbacks such as difficulty again, does not meet shortcomings such as the strategy of sustainable development.Therefore, the reform traditional landfill process, the correlation technique of landfill yard stabilization process is quickened in research, has become extremely urgent problem.
Refuse landfill is the ecosystem of a complicacy, in the process of its stabilization, carrying out multiple Biochemical processes, and the vital movement of mikrobe is the basis that these processes are carried out smoothly in the landfill yard.The organism that contains many slow degradeds in the landfill waste is an example with Mierocrystalline cellulose in the landfill waste, has content height (account for plant dry matter 40~60%); Characteristics such as biological degradation is slow, in the landfill waste therefore original indigenous microorganism is difficult to effectively with its decomposition, and only rely on; In the garbage loading embeading system, introduce external source superior microorganism with specific function; To improve the kind and the concentration of effective microbe in the landfill yard, quicken the wherein growth and breeding of dominant microflora, improve the treatment effect of original biological treatment system; Quicken the stabilization process of landfill waste, be of great practical significance.
The present invention is based on recharging this novel garbage loading embeading technology of type bioreactor landfill; A kind of complex microbial inoculum and making method of quickening the landfill waste stabilization is provided; Utilize biological reinforced means to improve the microenvironment of landfill waste, accelerate the biological degradation of rubbish in the landfill yard, quicken the stabilization process of landfill yard; Improve its processing efficiency; Quicken subduing of organic pollutant in the percolate, reduce the difficulty of its subsequent disposal, make the sanitary landfill of domestic refuse meet the strategy of sustainable development more.
Summary of the invention
The object of the present invention is to provide a kind of function composite fungus agent that can quicken the domestic refuse degradation process, quicken the purpose that domestic refuse is degraded fast in the refuse landfill, a kind of function composite fungus agent that quickens the domestic refuse degraded is provided for reaching; Has good synergy between microbial population; The degradation capability that not only common organic waste in the domestic refuse is had efficient stable, and strong to the flexibility and the contamination resistance of varying environment, and microbial inoculum can quicken the stabilization process of landfill waste; Also can be applicable to consumer garbage compost; Improve fermentation efficiency, shorten the compost time, reduce processing cost.
For realizing above-mentioned target, the technical scheme that the present invention adopts is:
A kind of functional composite biological agent for high-efficiency degradation of household refuses and application thereof, microbial inoculum is made up of Mierocrystalline cellulose efficient degrading bacteria and percolate COD degradation bacteria.Mierocrystalline cellulose efficient degrading bacteria: percolate COD degradation bacteria (the turbidity OD of bacteria suspension 600Be 0.8 ± 0.1) constitute according to volume(tric)fraction=1~5: 1 (V/V).Said Mierocrystalline cellulose efficient degrading bacteria is by the bacillus sp. of high cellulase-producing, cellulomonas cartae Cellulomonas sp., and wooden mould Trichoderma sp., aspergillus Aspergillus sp. was in 3: 3: 2: 2 ratio is formed; Percolate COD degradation bacteria is by the pseudomonas Pseudonomas sp. of COD in effective degraded percolate, Flavobacterium Flavobacterium sp., and acinetobacter calcoaceticus Acinetobacter sp. forms in 7: 2: 1 ratio.
The present invention also aims to provide a kind of making method of functional composite biological agent for high-efficiency degradation of household refuses, its concrete steps are:
(1) activation of bacterial classification: with above-mentioned bacterial classification; Adopt the method that constantly increases percolate concentration that bacterial classification is carried out the orientation domestication; To the mikrobe enlarged culturing of carrying out after the domestication; The microbionation of Mierocrystalline cellulose efficient degradation is mixed culture in by the beef-protein medium of percolate preparation, and fungi is inoculated in by mixed culture in the percolate preparation potato culture; Percolate COD degradation bacteria is inoculated in by mixed culture in the beef-protein medium of percolate preparation, treats that the microorganism growth situation tends towards stability, and promptly obtains the mixed training thing of bacterial classification.The actication of culture process adopts the pressure substratum of percolate preparation, can effectively tame mikrobe, makes bacterial classification adapt to the toxicity of percolate, and the environment of adaptation landfill yard is easy in landfill waste, grow.
(2) making of microbial inoculum: the culture of above-mentioned Mierocrystalline cellulose efficient degrading bacteria moral bacterium and fungi is mixed, regulate the turbidity OD of bacteria suspension 600Be 0.8 ± 0.1, regulate the turbidity OD of the mixed training thing of percolate COD degradation bacteria simultaneously 600Be 0.8 ± 0.1, then with the mixed training thing of Mierocrystalline cellulose efficient degrading bacteria and percolate COD degradation bacteria according to volume(tric)fraction 1~5: the ratio mixing of 1 (V/V) is functional composite biological agent for high-efficiency degradation of household refuses.
The present invention also aims to, explore a kind of application of functional composite biological agent for high-efficiency degradation of household refuses, adopt following means that domestic refuse is handled.
At the landfill initial stage; Is 0.5~8.0% ratio in landfill waste in mass ratio with said functional composite biological agent for high-efficiency degradation of household refuses, and that said functional composite biological agent for high-efficiency degradation of household refuses directly makes an addition to is aerobic, accurate aerobic, anaerobism refuse landfill or be inoculated in garbage compost and dynamically ferment.
The Mierocrystalline cellulose efficient degrading bacteria can quicken the landfill waste biological degradation; The percolate COD degradation bacteria hardly degraded organic substance in the percolate of can effectively degrading; Have good synergy between bacterial classification, the synergy of the two can effectively be quickened the stabilization process of landfill waste.Functional composite biological agent for high-efficiency degradation of household refuses of the present invention not only has the degradation capability of efficient stable to common organic waste in the rubbish; And strong to the flexibility and the contamination resistance of varying environment, have broad application prospects at aspects such as organic waste processing and disposal and recyclings.
Description of drawings is following:
The change trend curve figure of COD in the aerobic landfill waste percolate of Fig. 1
The aerobic landfill waste diafiltration of Fig. 2 liquid measure is graphic representation over time
The change curve of facile hydrolysis thing in the aerobic landfill waste of Fig. 3 different times
The change curve of content of cellulose in the aerobic landfill waste of Fig. 4 different times
Embodiment:
Embodiment 1 functional composite biological agent for high-efficiency degradation of household refuses is applied to the aerobic rubbish landfill yard
See also accompanying drawing 1,2,3,4.
Embodiment 1 carries out according to following steps:
1. make rubbish efficient degradation function composite fungus agent:
Microbial inoculum is made up of Mierocrystalline cellulose efficient degrading bacteria and percolate COD degradation bacteria.The Mierocrystalline cellulose efficient degrading bacteria is by the bacillus sp. of high cellulase-producing, cellulomonas cartae Cellulomonas sp., and wooden mould Trichoderma sp., aspergillus Aspergillus sp. was in 3: 3: 2: 2 ratio is formed; Percolate COD degradation bacteria is by the pseudomonas Pseudonomas sp. of COD in effective degraded percolate, Flavobacterium Flavobacterium sp., and acinetobacter calcoaceticus Acinetobacter sp. forms in 7: 2: 1 ratio.
Adopt the method that constantly increases percolate concentration that above-mentioned bacterial classification is carried out the orientation domestication, make bacterial classification adapt to the toxicity of percolate gradually; To the mikrobe enlarged culturing of carrying out after the domestication, treat that each growth situation is stable after, with the Mierocrystalline cellulose efficient degrading bacteria: percolate COD degradation bacteria (bacteria suspension OD 600Be 0.8 ± 0.1) be that 2: 1 mixed is functional composite biological agent for high-efficiency degradation of household refuses, subsequent use.
2. garbage loading embeading: the content of each composition is according to China's domestic waste component ratio design (component of refuse is seen table 1) in the landfill waste; The rubbish sample is taken near the fresh domestic refuse of the several garbage transfer stations Southwest Jiaotong University; Landfill is in simulation refuse landfill experimental installation behind the mixing; Experimental group is 2.0% ratio adding mentioned microorganism microbial inoculum according to functional composite biological agent for high-efficiency degradation of household refuses and landfill waste mass ratio; Control group adds the isopyknic tap water of microbiobacterial agent, and two groups are all adopted aeration mode is that landfill yard is supplied with oxygen, regularly carries out percolate simultaneously and recharges; Respectively facile hydrolysis thing and cellulosic percentage composition in the percolate output in experimental group and the control group rubbish, percolate COD and the rubbish are monitored, experimental result is seen accompanying drawing 1 ~ 4.
Table 1 simulation landfill yard waste component content
210d has been carried out in experiment; Adding the experimental group of function composite fungus agent and the variation of control group leachate quality and component of refuse and the result of settling height through contrast can find; The experiment initial stage is owing to be easy to biodegradable organic quick degraded in the domestic refuse, causes that decline gradually subsequently fast rise all appears, in COD in the percolate that experimental group and control group produced; The COD of whole experiment experimental group is lower than control group always, helps the processing of follow-up percolate; Facile hydrolysis thing and cellulosic percentage composition also are lower than control group in the landfill waste of experimental group; Simultaneously; The whole landfill cycle; The settling height of experimental group is higher than control group always, and the 41d that is deposited in of experimental group landfill waste reached 85.2% of its final settlement height, and control group only reaches 73.2% of final settlement height at 41d; Settling height increases by 15.2% than control group during to the experiment end, and the minimizing of rubbish is obvious; The rubbish material object of landfill 210d is when also illustrative experiment finishes; Remaining organic substance is less than control group greatly in the experimental group landfill waste, mainly is non-biodegradable rubbish such as plastics bag in the experimental group, and control group rubbish except plastics bag etc., also contain a large amount of can rotten rubbish; From color judgment; Experimental group is a black, and control group slightly is yellow, and also the rotten degree of illustrative experiment group residue rubbish is higher.The function composite bacteria can not only quicken to be prone in the landfill waste biological degradation of degradation product; Also accelerated the biodegradation process of difficult degradation such as xylogen, Mierocrystalline cellulose thing in the landfill waste; More thorough to difficult degradation organic waste utilization in the landfill yard; Thereby quicken the stabilization process of refuse landfill, make waste reductionization more obvious, thereby effectively improve the utilising efficiency of landfill yard; In addition, the function composite bacteria can also effectively be removed organic pollutant in the percolate, reduces the organic loading of percolate, thereby reduces the difficulty of percolate subsequent disposal.
Embodiment 2 functional composite biological agent for high-efficiency degradation of household refuses are applied to the semi-aerobic rubbish landfill
Embodiment 2 carries out according to following steps:
1. the making method of rubbish efficient degradation function composite fungus agent is with embodiment 1.
2. garbage loading embeading: the content of each composition is according to China's domestic waste component ratio design (with embodiment 1) in the landfill waste; The rubbish sample is taken from the fresh domestic refuse of several garbage transfer stations; Landfill is in simulation refuse landfill experimental installation behind the mixing; Experimental group is 2.0% ratio interpolation mentioned microorganism microbial inoculum according to microbial inoculum and landfill waste mass ratio; Adopting the mode of natural ventilation is the oxygen supply of semi-aerobic rubbish landfill yard, respectively the percolate output in experimental group and the control group rubbish, percolate COD and settling height is monitored.
The experimental result of semi-aerobic rubbish landfill and aerobic land-fill method have similar result; The amount of the percolate that experimental group produced and COD load all is lower than control group in the whole experiment; Wherein organic degradation speed is faster than control group, and settling height explains that also greater than control group microbiobacterial agent can reduce the organic loading of percolate; Quicken the stabilization process of semi-aerobic rubbish landfill yard, improve the utilising efficiency of landfill yard.
Embodiment 3 functional composite biological agent for high-efficiency degradation of household refuses are applied to the anaerobism garbage loading embeading
Embodiment 3 carries out according to following steps:
The making of rubbish efficient degradation function composite fungus agent is with embodiment 1.The content of each composition is according to China's domestic waste component ratio design (with embodiment 1) in the landfill waste; The rubbish sample is taken from the fresh domestic refuse of several garbage transfer stations; Landfill is in simulation refuse landfill experimental installation behind the mixing; Experimental group is 4.0% ratio interpolation mentioned microorganism microbial inoculum according to microbial inoculum and landfill waste mass ratio; Adopt the mode of anaerobism landfill, respectively the percolate output in experimental group and the control group rubbish, percolate COD and settling height are monitored, 450d has been carried out in experiment.Experimental result and aerobic, accurate aerobic landfill are similar; The amount of the percolate that experimental group produced and COD load all is lower than control group in the whole experiment, and wherein organic degradation speed is faster than control group, when finishing to experiment; Experimental group increases by 24.6% than control group settling height; Explain that microbiobacterial agent can quicken the stabilization process of anaerobism refuse landfill, improve the utilising efficiency of landfill yard, reduce the subsequent disposal difficulty of percolate.
Embodiment 4 domestic refuse aerobic composts
Embodiment 4 carries out according to following steps:
The making of rubbish efficient degradation function composite fungus agent is with embodiment 1.Compost rubbish sample is taken near the fresh domestic waste of the garbage transfer station in southwestern Shanghai Communications University; After materials such as wherein glass, metal, plastics are carried out sorting; Residue degradable organic waste carries out compost (the initial C/N of compost rubbish is 30.5), and in the garbage compost analogue experiment installation of packing into behind the mixing, experimental group is 2.0% ratio interpolation mentioned microorganism microbial inoculum according to mass ratio; Adopt the oxygen supply of aeration airy mode, and according to the regular turning of compost temperature.Monitor total organic carbon (TOC), total organic nitrogen (TN) and the heap temperature of rubbish simultaneously, represent rotten degree with C/N.The result shows that the envrionment temperature of experiment is 28 ℃, and experimental group has experienced the middle temperature phase of 2d; Get into the pliotherm period rapidly, heap temperature is elevated to more than 55 ℃ and keeps 3d, and reaches 62.3 ℃ in 3d; Meet national compost hygienic standard, and control group just arrives 55.0 ℃ to 6d, the time length is 1d only; The pliotherm period of experimental group explains that than the control group longer duration mikrobe utilizes the efficient of compost rubbish high in the experimental group of adding dominant bacteria, and mikrobe is more strengthened the biological degradation of rubbish; C/N also shows; Take the lead in initial C/N by compost rubbish of experimental group 30.5 reduces to 16.0; Estimate the rotten degree of compost according to T value ; When the T of experimental group value was 0.53, control group T value was 0.67, and the illustrative experiment group is become thoroughly decomposed in advance; Simultaneously, from the apparent judgement of composting production, during to the experiment end, the experimental group compost presents chocolate, and is loose, and argillous fragrance is arranged, and reached apparent and become thoroughly decomposed, and control group still is a tawny that water ratio is higher, do not reach apparent and becomes thoroughly decomposed.Treating urban domestic garbage using high efficiency microbiological composite bacterial agent can improve rubbish fermentation efficient, shortens the compost time, reduces processing cost.
Find in the experiment, under the constant basically situation of other condition, Mierocrystalline cellulose efficient degrading bacteria: percolate COD degradation bacteria (the turbidity OD of bacteria suspension 600Be 0.8 ± 0.1) when constituting, can both realize the object of the invention according to volume(tric)fraction=1~5: 1 (V/V).
Same, functional composite biological agent for high-efficiency degradation of household refuses is 0.5~8.0% ratio in landfill waste in mass ratio, can both reach the object of the invention, if consider the factor of economy, 2.0~4.0% the ratio of getting is preferable.

Claims (3)

1. functional composite biological agent for high-efficiency degradation of household refuses; It is characterized in that: said microbial inoculum is made up of Mierocrystalline cellulose efficient degrading bacteria and percolate COD degradation bacteria, and Mierocrystalline cellulose efficient degrading bacteria and percolate COD degradation bacteria are according to volume(tric)fraction 1~5: 1 composition of proportions; Said Mierocrystalline cellulose efficient degrading bacteria is by the genus bacillus (Bacillus sp.) of high cellulase-producing, cellulomonas cartae (Cellulomonas sp.), and wood mould (Trichoderma sp.), aspergillus (Aspergillus sp.) is in 3: 3: 2: 2 ratio composition; Percolate COD degradation bacteria is by the pseudomonas (Pseudonomas sp.) of COD in effective degraded percolate, Flavobacterium (Flavobacterium sp.), and acinetobacter calcoaceticus (Acinetobacter sp.) is formed in 7: 2: 1 ratio.
2. the making method of the described functional composite biological agent for high-efficiency degradation of household refuses of claim 1 is characterized in that:
(1) activation of bacterial classification: adopt the method that constantly increases percolate concentration, to the said bacterial classification of claim 1 being carried out the orientation domestication; Then the mikrobe after the domestication is carried out enlarged culturing, the microbionation of Mierocrystalline cellulose efficient degradation is mixed culture in by the beef-protein medium of percolate preparation, and fungi is inoculated in by mixed culture in the percolate preparation potato culture; Percolate COD degradation bacteria is inoculated in by mixed culture in the beef-protein medium of percolate preparation, treats that the microorganism growth situation tends towards stability, and promptly obtains the mixed training thing of bacterial classification;
(2) making of microbial inoculum: the bacterium of above-mentioned Mierocrystalline cellulose efficient degrading bacteria and the culture of fungi are mixed, regulate the turbidity OD of bacteria suspension 600Be 0.8 ± 0.1, regulate the turbidity OD of the culture of percolate COD degradation bacteria simultaneously 600Be 0.8 ± 0.1, then with the nutrient solution of Mierocrystalline cellulose efficient degrading bacteria and percolate COD degradation bacteria according to volume(tric)fraction 1~5: 1 ratio mixing is functional composite biological agent for high-efficiency degradation of household refuses.
3. the application of functional composite biological agent for high-efficiency degradation of household refuses; It is characterized in that: at the landfill initial stage; In 0.5~8.0% ratio of landfill waste quality, the said functional composite biological agent for high-efficiency degradation of household refuses of claim 1 directly made an addition to aerobic, accurate aerobic, anaerobism refuse landfill or be inoculated in garbage compost dynamically ferment.
CN201010100179XA 2010-01-22 2010-01-22 Functional composite biological agent for high-efficiency degradation of household refuses and application thereof Expired - Fee Related CN101787350B (en)

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