CN103468616B - Microbial agent for degrading garbage - Google Patents
Microbial agent for degrading garbage Download PDFInfo
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- CN103468616B CN103468616B CN201310434210.7A CN201310434210A CN103468616B CN 103468616 B CN103468616 B CN 103468616B CN 201310434210 A CN201310434210 A CN 201310434210A CN 103468616 B CN103468616 B CN 103468616B
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Abstract
The invention relates to the technical field of microorganisms, and particularly provides a microbial agent which can efficiently degrade domestic garbage, particularly kitchen garbage, by the synergistic promotion effect of bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, wherein the degradation rate reaches 80%, and the microbial agent is beneficial to improving the living environment and reducing the environmental pollution.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a microbial agent for degrading garbage.
Background
With the development of economy, the explosion of population and the improvement of the living standard of people, the yield of urban domestic garbage is increased day by day, and the national protocol of twenty-one world in China indicates that: "the domestic garbage stock in the whole country over the years reaches more than 60 hundred million tons, and 200 cities are immersed in the garbage enclosure. According to statistics, the urban garbage growth rate in China is more than 10 percent and exceeds the average annual growth rate of 8.42 percent in the world. How to treat the huge municipal solid waste becomes a problem of wide attention all over the world. Meanwhile, the problems of environmental pollution caused by household garbage, deterioration of human settlement and the like also become common concerns of all countries in the world, and the problems also become barriers for restricting the sustainable development of the society and the economy in China. Therefore, the development of garbage treatment toward reduction, harmlessness and recycling is a measure.
At present, the main modes for treating garbage at home and abroad comprise sanitary landfill, composting and incineration, wherein the former two treatment modes belong to biological treatment technologies. Specifically, the biological garbage treating technology is a biochemical process of stabilizing garbage gradually by means of carrying out a series of biochemical reactions on the microbes inherent or added externally in the urban domestic garbage under a certain control condition to release the metabolic capacity of unstable organic matters in the garbage or convert the unstable organic matters into new cell substances.
The microorganisms such as bacteria, actinomycetes and fungi widely distributed in the nature controllably promote the biochemical process of converting biodegradable organic matters into stable humus, finally form similar humus, and can be used as a fertilizer or a soil conditioner. The composting technology is an important way for realizing the reclamation and the harmlessness of the municipal refuse, can kill pathogenic bacteria in the refuse, effectively treat organic matters in the refuse, increase organic components in soil, can produce organic fertilizers and is beneficial to increasing the agricultural yield.
However, the garbage is degraded only by the original microorganisms in the nature, the period is too long, and the efficiency is poor, so that the method of adding the high-concentration microbial inoculum is generally adopted for the garbage treatment at present, the treatment efficiency can be greatly improved, and the environment is not damaged. However, the selection of strains is the key point for restricting the garbage treatment efficiency, and is one of the hot spots of the current domestic and foreign researches.
Disclosure of Invention
The invention provides a microbial agent for degrading garbage, which can effectively degrade organic substances in household garbage, especially kitchen garbage, through the synergistic promotion effect among strains, thereby obviously reducing the odor of the garbage and improving the living environment.
The invention relates to a microbial agent, which is prepared from bacillus cereus (Bacillus cereus)Bacillus cereus) Lactobacillus acidophilus (a)Lactobacillus acidophilus) Bacillus stearothermophilus (B.stearothermophilus) (B.stearothermophilus)Bacillus stearothermophilus) Pseudomonas pseudoalcaligenes (A), (B)Pseudomonas pseudoalcaligenes) And Saccharomyces cerevisiae (Saccharomyces cerevisiae) And (4) forming.
The microbial agent comprises the following components in percentage by mass: 30-100 parts of bacillus cereus, 300 parts of lactobacillus acidophilus, 150 parts of bacillus stearothermophilus, 50-120 parts of pseudoalcaligenes and 80-150 parts of saccharomyces cerevisiae.
The components of the microbial agent and the mass ratio thereof are respectively preferably as follows: 80 parts of bacillus cereus, 230 parts of lactobacillus acidophilus, 120 parts of bacillus stearothermophilus, 85 parts of pseudomonas pseudoalcaligenes and 110 parts of saccharomyces cerevisiae.
The components of the microbial agent are preferably bacillus cereus CGMCC1.2238, lactobacillus acidophilus CGMCC1.1878, bacillus stearothermophilus CGMCC1.1145, pseudomonas pseudoalcaligenes CGMCC1.2328 and saccharomyces cerevisiae CGMCC 2.3868.
The viable bacteria count of Bacillus cereus, Lactobacillus acidophilus, Bacillus stearothermophilus, Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae in the above microbial agent is 108-1010CFU/g。
In another aspect, the present invention provides a method for producing the microbial agent, comprising the following steps:
1) respectively fermenting bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae by adopting high-density liquid;
2) the spore content is as high as 10 by adopting a spray drying method9-1010CFU/g of ultra-concentrated microbial powder;
3) weighing 30-100 parts of the bacillus cereus prepared in the step 1), 300 parts of lactobacillus acidophilus, 100-150 parts of bacillus stearothermophilus, 50-120 parts of pseudoalcaligenes and 80-150 parts of saccharomyces cerevisiae according to the proportion, and uniformly mixing in a stirrer;
4) and (3) drying the uniformly mixed bacterium powder in an air drying cylinder to obtain the microbial agent.
Advantageous effects
The microbial agent disclosed by the invention can efficiently degrade household garbage, especially kitchen garbage, by the synergistic promotion effect of bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, and the degradation rate reaches 80%. The invention also provides a preparation method of the microbial inoculum, which has simple technical process, is suitable for the existing technology and fermentation equipment and is easy to widely popularize. The method for degrading the household garbage provided by the invention has the advantages of simple operation, easy mastering and good garbage degradation effect, and is beneficial to quick garbage treatment and environmental protection.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications can be made by those skilled in the art after reading the disclosure of the present invention, and equivalents fall within the scope of the appended claims.
The various reagents and devices related to the invention can be selected from any one of the reagents and devices which are commercially available; the invention relates to a bacillus cereus strain, which is purchased from China general microbiological culture collection center with the collection number of CGMCC 1.2238; the lactobacillus acidophilus is purchased from China general microbiological culture collection center with the collection number of CGMCC 1.1878; the Bacillus stearothermophilus is purchased from China general microbiological culture collection center with the collection number of CGMCC 1.1145; pseudomonas pseudoalcaligenes is purchased from China general microbiological collection center with the collection number of CGMCC 1.2328; the saccharomyces cerevisiae is purchased from China general microbiological culture collection center with the collection number of CGMCC 2.3868.
Example 1
A microbial agent comprises the following components in parts by mass: 100 parts of bacillus cereus, 100 parts of lactobacillus acidophilus, 150 parts of bacillus stearothermophilus, 50 parts of pseudoalcaligenes and 150 parts of saccharomyces cerevisiae.
The viable bacteria count of Bacillus cereus, Lactobacillus acidophilus, Bacillus stearothermophilus, Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae in the above microbial agent is 108-1010CFU/g。
The production method of the microbial compound fertilizer comprises the following steps:
1) respectively fermenting bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae by adopting high-density liquid;
2) the spore content is as high as 10 by adopting a spray drying method9-1010CFU/g of ultra-concentrated microbial powder;
3) weighing 100 parts of bacillus cereus, 100 parts of lactobacillus acidophilus, 150 parts of bacillus stearothermophilus, 50 parts of pseudoalcaligenes and 150 parts of saccharomyces cerevisiae prepared in the step 1) in proportion, and uniformly mixing in a stirrer;
4) and (3) drying the uniformly mixed bacterium powder in an air drying cylinder to obtain the microbial agent.
Example 2
A microbial agent comprises the following components in parts by mass: 30 parts of bacillus cereus, 300 parts of lactobacillus acidophilus, 100 parts of bacillus stearothermophilus, 120 parts of pseudomonas pseudoalcaligenes and 80 parts of saccharomyces cerevisiae.
The viable bacteria count of Bacillus cereus, Lactobacillus acidophilus, Bacillus stearothermophilus, Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae in the above microbial agent is 108-1010CFU/g。
The production method of the microbial compound fertilizer comprises the following steps:
1) respectively fermenting bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae by adopting high-density liquid;
2) the spore content is as high as 10 by adopting a spray drying method9-1010CFU/g of ultra-concentrated microbial powder;
3) weighing 30 parts of the bacillus cereus prepared in the step 1), 300 parts of lactobacillus acidophilus, 100 parts of bacillus stearothermophilus, 120 parts of pseudoalcaligenes and 80 parts of saccharomyces cerevisiae in proportion, and uniformly mixing in a mixer;
4) and (3) drying the uniformly mixed bacterium powder in an air drying cylinder to obtain the microbial agent.
Example 3
A microbial agent comprises the following components in parts by mass: 80 parts of bacillus cereus, 230 parts of lactobacillus acidophilus, 120 parts of bacillus stearothermophilus, 85 parts of pseudomonas pseudoalcaligenes and 110 parts of saccharomyces cerevisiae.
The viable bacteria count of Bacillus cereus, Lactobacillus acidophilus, Bacillus stearothermophilus, Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae in the above microbial agent is 108-1010CFU/g。
The production method of the microbial compound fertilizer comprises the following steps:
1) respectively fermenting bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, pseudomonas pseudoalcaligenes and saccharomyces cerevisiae by adopting high-density liquid;
2) the spore content is as high as 10 by adopting a spray drying method9-1010CFU/g of ultra-concentrated microbial powder;
3) weighing 80 parts of the bacillus cereus prepared in the step 1), 230 parts of lactobacillus acidophilus, 120 parts of bacillus stearothermophilus, 85 parts of pseudoalcaligenes and 110 parts of saccharomyces cerevisiae in proportion, and uniformly mixing in a stirrer;
4) and (3) drying the uniformly mixed bacterium powder in an air drying cylinder to obtain the microbial agent.
Example 4 application of microbial inoculum in degrading household garbage
The domestic garbage selects leftovers in a canteen;
putting the microbial agent into a garbage machine according to 10% of the weight of the garbage, and uniformly stirring at 200 rpm; fermenting for 24 hours, adding 10 percent of new garbage with the initial weight, and uniformly stirring at 200 rpm; the above operation was repeated 3 times.
The initial weight of the garbage is 50kg, and the weight of the garbage after treatment is 45 kg.
The garbage degradation rate = (initial weight-weight after treatment + weight of microbial agent)/initial weight × 100%.
Through calculation, the degradation rate of the garbage treated by the microbial agent is as high as 80%.
Claims (3)
1. A microbial agent comprises Bacillus cereus CGMCC1.2238, Lactobacillus acidophilus CGMCC1.1878, Bacillus stearothermophilus CGMCC1.1145, Pseudomonas pseudoalcaligenes CGMCC1.2328 and Saccharomyces cerevisiae CGMCC 2.386; the mass ratio of each component is as follows: 30-100 parts of bacillus cereus, 300 parts of lactobacillus acidophilus, 150 parts of bacillus stearothermophilus, 50-120 parts of pseudoalcaligenes and 80-150 parts of saccharomyces cerevisiae.
2. The microbial inoculant according to claim 1, wherein the bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, or paediatric bacteria are selected from the group consisting of bacillus cereus, lactobacillus acidophilus, bacillus stearothermophilus, and bacillus cereusThe viable count of the alkaline pseudomonas and the saccharomyces cerevisiae are both 108-1010CFU/g。
3. Use of the microbial inoculant of claim 1 or 2 in waste treatment.
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| CN107760616A (en) * | 2017-07-25 | 2018-03-06 | 青岛海芬健康产业科技有限公司 | A kind of microbial bacterial agent for rubbish from cooking of degrading and preparation method thereof |
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| CN101269227B (en) * | 2007-03-21 | 2011-07-27 | 北京世纪星伊埃姆生物技术研究所 | Biological agent for purifying air, preparation method and uses thereof |
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