CN110577905A - Compound microbial agent and preparation method and application thereof - Google Patents
Compound microbial agent and preparation method and application thereof Download PDFInfo
- Publication number
- CN110577905A CN110577905A CN201910744378.5A CN201910744378A CN110577905A CN 110577905 A CN110577905 A CN 110577905A CN 201910744378 A CN201910744378 A CN 201910744378A CN 110577905 A CN110577905 A CN 110577905A
- Authority
- CN
- China
- Prior art keywords
- microbial agent
- compound microbial
- clostridium
- bacillus
- preservation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention relates to a compound microbial agent and application thereof, wherein the formula comprises the following components: the preparation method of the heat-resistant clostridium parvum wine, the thermophilic clostridium succinogenes, the brevibacillus borstelensis, the bacillus amyloliquefaciens, the pseudomonas fluorescens, the microbacterium oxydans, the mesophilic sphingosine bacillus and the bacillus subtilis comprises the following steps: firstly, preparing a compound microbial agent culture medium, wherein the mass percent of the components is as follows: tryptone 1%, yeast extract 0.5%, sodium chloride 1%, pH of the culture medium 7.0; then inoculating and adsorbing, respectively and independently fermenting each strain in the compound microbial agent culture medium, then mixing the strains in proportion, adsorbing on an adsorbing material, and then drying properly to obtain the compound microbial agent.
Description
Technical Field
the invention relates to the field of biological agents, and particularly relates to a neutral environment-friendly cleaning agent and a preparation method thereof.
Background
China, as a large population country, is greatly demanding in terms of the number of toilets and, at the same time, is increasing with the level of living of people. The number of visitors in scenic spots in China increases year by year, the demand of public toilets in daily life is increased sharply, in addition to the shortage of water resources in recent years, the government has low carbon and environmental protection requirements for various industries, and the application of the waterless ecological toilet is more and more adapted to the demand of various social circles, so that the waterless ecological toilet is greatly promoted.
The unhydrated ecological toilet is not limited by water resources, the excrement and urine are separated from urine from the source, the solid organic fertilizer and the liquid organic fertilizer are generated after the excrement and urine are subjected to microbial treatment by adopting an efficient microbial treatment technology, the water resources are saved, the personnel cost caused by excrement treatment is greatly reduced, and the unhydrated ecological toilet has a wide application prospect.
Because the microbial inoculum selected by the current ecological toilet has low fermentation efficiency and has insufficient decomposition of the components of the excrement grease and cellulose, the research and development of the high-efficiency microbial inoculum are imperative.
Disclosure of Invention
The present invention is directed to solving, to some extent, one of the technical problems in the related art described above. Therefore, the invention aims to provide the compound microbial agent and the preparation method and the application thereof, and the compound microbial agent has the advantages of low usage amount, cost reduction, short treatment period and good deodorization effect, generates solid organic fertilizer and liquid organic fertilizer after treatment, can be used as fertilizer for greening, and realizes on-site recycling and harmlessness.
In a first aspect, the present invention provides a complex microbial inoculant, which comprises: heat-resistant clostridium parvum, thermophilic clostridium succinicum, Brevibacillus borstelensis, Bacillus amyloliquefaciens, pseudomonas fluorescens, Microbacterium oxydans, sphingomonas thermophilus and bacillus subtilis.
The number ratio of the heat-resistant clostridium parvum, the thermophilic clostridium succinogenes, the brevibacillus borstelensis, the bacillus amyloliquefaciens, the pseudomonas fluorescens, the microbacterium oxydans, the sphingosine thermophilus and the bacillus subtilis in the formula of the composite microbial agent is 4:1:5:2:1:5: 1.
the bacillus amyloliquefaciens is purchased from the China general microbiological culture collection center, the address of a collection unit is No. 3 Xilu No.1 Beijing Kogyo, the facing-sunny district, and the collection number is as follows: CGMCC NO. 1769;
The pseudomonas fluorescens is purchased from the China general microbiological culture collection management center, the address of a collection unit is No. 3 of Xilu No.1 of Beijing Kogyo, the north Chen, and the collection number is as follows: CGMCC NO 13990;
The microbacterium oxydans is preserved in China general microbiological culture Collection center (CGMCC) at 03.01.2019, the address of the preservation unit is No. 3 Siro-1 Hospital, Beijing, the south China, and the preservation number is as follows: CGMCCNO.17104, preservation name Microbacterium oxydans;
The mesophilic sphingosine bacillus is preserved in China general microbiological culture Collection center at 03.01.2019, the preservation unit is No. 3 of Xilu No.1 of Beijing university facing Yang district, the preservation number is as follows: CGMCC NO.17105, deposited under the name of Sphingobacterium thermophilum, Sphingobacterium thalophilum;
The bacillus subtilis is preserved in China general microbiological culture Collection center in 2019 at 03.01.03, the address of a preservation unit is No. 3 of Xilu No.1 of Beijing, the south facing Yang district, and the preservation number is as follows: CGMCC NO.17106, preservation name of Bacillus subtilis.
in a second aspect, the present invention provides a method for preparing a complex microbial inoculant, comprising the following steps:
S1, preparing a compound microbial agent culture medium, wherein the compound microbial agent culture medium comprises the following components in percentage by mass: tryptone 1%, yeast extract 0.5%, sodium chloride 1%, pH of the medium 7.0.
S2, inoculating and adsorbing, namely, respectively and independently fermenting the heat-resistant clostridium parvum, the thermophilic clostridium succinogenes, the brevibacillus borstelensis, the bacillus amyloliquefaciens, the pseudomonas fluorescens, the microbacterium oxydans, the mesophilic sphingosine bacillus and the bacillus subtilis in the culture medium of S1, then mixing the materials in proportion, adsorbing the materials on an adsorbing material, and then properly drying the materials to obtain the compound microbial agent.
Preferably, the compound microbial agent is liquid, and the viable bacteria number of the bacterial liquid is 108-109Billions/ml.
The adsorbing material is bran or rice hull powder.
Preferably, the adsorbing material is bran with fineness of more than 50 meshes.
In a third aspect, the invention provides an application of a compound microbial agent, which can be used for decomposing excrement, the compound microbial agent prepared by the invention is put into solid reactors of 3 toilet positions of an anhydrous ecological toilet according to the adding amount of 1 kg of each toilet position, after 3 months of operation, the residual amount of the excrement is greatly reduced, the excrement treatment period is shortened, the obvious deodorization effect is achieved, and the obtained residue compound biological fertilizer is standard.
The invention has the beneficial effects that:
The compound microbial agent can reduce the using amount of the microbial agent and reduce the production cost in the process of decomposing and using the excrement. Meanwhile, the residual quantity of the excrement is greatly reduced, the excrement treatment period is shortened, the personnel cost caused by excrement treatment is reduced, the obvious deodorization effect is achieved, the excrement and urine are subjected to microbial treatment to generate solid organic fertilizer and liquid organic fertilizer which can be used as fertilizer for greening, and the on-site recycling, harmless treatment, reduction and low cost are realized.
the specific implementation mode is as follows:
The compound bacterial liquid of the invention adopts the following microorganisms: clostridium pyrum (Clostridium thermopalmatum) capable of producing cellulase and degrading cellulose efficiently; thermophilic clostridium succinogenes (clostridium thermosuccinogenes), also a strain of cellulose degrading bacteria; brevibacillus borstelensis (Brevibacillus borstelensis) which can produce mannase, amylase and the like and has obvious inhibition effect on various original fungi; bacillus amyloliquefaciens (Bacillus amyloliquefaciens) can produce various bioactive substances such as amylase, protease, glucanase, hemicellulase, pentosanase, phytohormone and the like, and can degrade ammonia nitrogen and nitrite; pseudomonas fluorescens (Pseudomonas fluorescens) can generate lipase and has better decomposition effect on fatty substances; microbacterium oxydans (Microbacterium oxydans), fermentable glucose, sucrose, and the like; sphingosine bacilli mesophilic (Sphingobacterium thallophilum), uricase producing enzyme; bacillus subtilis produces various amylases and proteases, and is mainly applied to fermenting and decomposing various organic fertilizers (manure) and promoting organic matters to be decomposed into humus.
The following examples are given to illustrate the practice of the present invention.
Example 1: preparation of composite bacterial liquid
Fermenting strains: mixing and culturing heat-resistant clostridium parvum, thermophilic clostridium succinicum and brevibacillus borstelensis, and fermenting in a fermentation tank. The medium was prepared as follows: 0.5 percent of peptone, 0.1 percent of yeast extract, 1 percent of cellulose (filter paper), 0.5 percent of sodium chloride, 0.5 percent of calcium carbonate, 8.0 percent of pH, static culture at 50 ℃ to ensure that DO is between 0.02 and 0.4, and degradation of more than 50 percent of straws after 3 days of fermentation. Five strains of bacillus amyloliquefaciens, pseudomonas fluorescens, microbacterium oxydans, sphingosine bacillus mesophilic and bacillus subtilis are respectively and independently subjected to shaking table amplification culture and fermentation tank fermentation. The formula of the culture medium is as follows: tryptone 1%, yeast extract 0.5%, sodium chloride 1%, pH of the culture medium 7.0, shaking culture at 30 ℃ for 24 h. The number of live bacteria of the fermented bacteria liquid reaches 25-30 hundred million/ml.
Example 2: preparation of bacterial preparation product
The adsorbent of the invention can be any adsorbent for preparing a microbial agent, and is preferably bran, wherein the bran is prepared by crushing freshly processed dry wheat bran, and the fineness of the bran is more than 50 meshes.
The composite bacterial liquid prepared in the example 1 is adsorbed by bran in an adsorption stirring device, and the heat-resistant clostridium pajiu, the thermophilic clostridium succinate and the brevibacillus borstelensis cultured in a mixing way are mixed with the separately cultured bacillus amyloliquefaciens, the pseudomonas fluorescens, the microbacterium oxydans, the sphingosine mesophilic bacillus and the bacillus subtilis according to the volume ratio of 10:2:1:5 (L), so that the number ratio of the heat-resistant clostridium pajiu, the thermophilic clostridium succinicu, the brevibacillus borstelensis, the bacillus amyloliquefaciens, the pseudomonas fluorescens, the microbacterium oxydans, the sphingosine mesophilic bacillus and the bacillus subtilis in the formed composite bacterial liquid is about 4:1:5:2:1:5:1: 5. Then adsorbing the prepared mixed bacterial liquid and rice hull powder, wherein the adsorption ratio volume (L)/mass (kg) is bacterial liquid: and (3) uniformly stirring the rice hull powder at a ratio of 1: 2-4, and airing in a ventilating way, wherein the water content is controlled within 15%.
example 3: use of bacteria preparation product in ecological toilet
The compound microbial agent obtained by the method is put into solid reactors of 3 toilets of an anhydrous ecological toilet according to the adding amount of 1 kg of each toilet, after 3 months of operation, under the action of the microbial agent, the residual amount of excrement is greatly reduced, the excrement treatment period is shortened, the deodorization effect is obvious, the obtained residue compound bio-fertilizer is standard, and the detection result is as follows:
the present invention has been described in detail, and the principle and embodiments of the present invention are explained herein by using specific examples, which are only used to help understand the method and the core idea of the present invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the principle of the present invention, and these changes and modifications also fall into the protection scope of the appended claims.
Claims (7)
1. the compound microbial agent is characterized by comprising the following components in percentage by weight: heat-resistant clostridium parvum, thermophilic clostridium succinicum, Brevibacillus borstelensis, Bacillus amyloliquefaciens, pseudomonas fluorescens, Microbacterium oxydans, sphingomonas thermophilus and bacillus subtilis.
2. the compound microbial agent as claimed in claim 1, wherein the amount ratio of Clostridium parvum, Clostridium thermophilum succinate, Brevibacillus borstelensis, Bacillus amyloliquefaciens, Pseudomonas fluorescens, Microbacterium oxydans, Sphingobacterium mesophilic and Bacillus subtilis in the formula is 4:1:5:2:1:5:1: 5.
3. The preparation method of the compound microbial agent is characterized by comprising the following steps:
S1, preparing a compound microbial agent culture medium, wherein the compound microbial agent culture medium comprises the following components in percentage by mass: tryptone 1%, yeast extract 0.5%, sodium chloride 1%, pH of the medium 7.0.
S2, inoculating and adsorbing, namely, respectively and independently fermenting the heat-resistant clostridium parvum, the thermophilic clostridium succinogenes, the brevibacillus borstelensis, the bacillus amyloliquefaciens, the pseudomonas fluorescens, the microbacterium oxydans, the mesophilic sphingosine bacillus and the bacillus subtilis in the culture medium of S1, then mixing the materials in proportion, adsorbing the materials on an adsorbing material, and then properly drying the materials to obtain the compound microbial agent.
4. the compound microbial agent according to claim 1 or 2, wherein the compound microbial agent is a liquid, and the viable bacteria amount of the bacterial liquid is 108-109Billions/ml.
5. The method for preparing a composite microbial inoculant according to claim 3, wherein the adsorbing material is bran or rice hull powder.
6. the application of the compound microbial agent is characterized in that the compound microbial agent can be used for decomposing excrement.
7. The complex microbial inoculant according to claim 1 or 2, wherein the bacillus amyloliquefaciens is purchased from the China general microbiological culture Collection center, the collection unit is No. 3 Xilu No.1 Beijing, Chaoyang, the collection number: CGMCC NO. 1769;
The pseudomonas fluorescens is purchased from the China general microbiological culture collection management center, the address of a collection unit is No. 3 of Xilu No.1 of Beijing Kogyo, the north Chen, and the collection number is as follows: CGMCC NO 13990;
The microbacterium oxydans is preserved in China general microbiological culture Collection center (CGMCC) at 03.01.2019, the address of the preservation unit is No. 3 Siro-1 Hospital, Beijing, the south China, and the preservation number is as follows: CGMCCNO.17104, preservation name Microbacterium oxydans;
The mesophilic sphingosine bacillus is preserved in China general microbiological culture Collection center at 03.01.2019, the preservation unit is No. 3 of Xilu No.1 of Beijing university facing Yang district, the preservation number is as follows: CGMCC NO.17105, deposited under the name of Sphingobacterium thermophilum, Sphingobacterium thalophilum;
the bacillus subtilis is preserved in China general microbiological culture Collection center in 2019 at 03.01.03, the address of a preservation unit is No. 3 of Xilu No.1 of Beijing, the south facing Yang district, and the preservation number is as follows: CGMCC NO.17106, preservation name of Bacillus subtilis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910744378.5A CN110577905A (en) | 2019-08-13 | 2019-08-13 | Compound microbial agent and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910744378.5A CN110577905A (en) | 2019-08-13 | 2019-08-13 | Compound microbial agent and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110577905A true CN110577905A (en) | 2019-12-17 |
Family
ID=68810654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910744378.5A Pending CN110577905A (en) | 2019-08-13 | 2019-08-13 | Compound microbial agent and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110577905A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625959A (en) * | 2020-12-28 | 2021-04-09 | 中农新科(苏州)有机循环研究院有限公司 | Thermophilic composite microbial inoculum for aerobic fermentation of organic wastes |
| CN113897310A (en) * | 2021-09-29 | 2022-01-07 | 中国科学院成都生物研究所 | Bacteria preparation for harmlessly treating dead pigs and using method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492621A (en) * | 2011-11-16 | 2012-06-13 | 天津工业生物技术研究所 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
| CN107099493A (en) * | 2017-07-06 | 2017-08-29 | 裴德修 | Compound microbial excrement decomposing agent and preparation method and excrement become thoroughly decomposed processing method |
| CN107400654A (en) * | 2017-08-03 | 2017-11-28 | 浙江工业大学 | A kind of recombination bacillus coli of alpha-glucosidase gene containing α and its application |
| CN108715820A (en) * | 2018-05-30 | 2018-10-30 | 水云天（天津）生物科技发展有限公司 | A method of the composite bacteria agent and utilization composite bacteria agent degradation VOCs of degradation VOCs |
| CN108865943A (en) * | 2018-07-18 | 2018-11-23 | 辽宁陆海石油装备研究院有限公司 | A kind of lavatory complex micro organism fungicide and its application |
-
2019
- 2019-08-13 CN CN201910744378.5A patent/CN110577905A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492621A (en) * | 2011-11-16 | 2012-06-13 | 天津工业生物技术研究所 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
| CN107099493A (en) * | 2017-07-06 | 2017-08-29 | 裴德修 | Compound microbial excrement decomposing agent and preparation method and excrement become thoroughly decomposed processing method |
| CN107400654A (en) * | 2017-08-03 | 2017-11-28 | 浙江工业大学 | A kind of recombination bacillus coli of alpha-glucosidase gene containing α and its application |
| CN108715820A (en) * | 2018-05-30 | 2018-10-30 | 水云天（天津）生物科技发展有限公司 | A method of the composite bacteria agent and utilization composite bacteria agent degradation VOCs of degradation VOCs |
| CN108865943A (en) * | 2018-07-18 | 2018-11-23 | 辽宁陆海石油装备研究院有限公司 | A kind of lavatory complex micro organism fungicide and its application |
Non-Patent Citations (2)
| Title |
|---|
| LIU YANG等: "Effect of low temperature compound starter on the biodiversity of cow muck compost in winter", SOIL AND FERTILIZER SCIENCES IN CHINA, no. 3, pages 94 - 98 * |
| 韩保安等: "猪粪除臭菌的筛选、复配以及培养条件的优化", 家畜生态学报, vol. 38, no. 12, pages 55 - 61 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625959A (en) * | 2020-12-28 | 2021-04-09 | 中农新科(苏州)有机循环研究院有限公司 | Thermophilic composite microbial inoculum for aerobic fermentation of organic wastes |
| CN112625959B (en) * | 2020-12-28 | 2023-06-23 | 中农新科(苏州)有机循环研究院有限公司 | Thermophilic composite microbial inoculant for aerobic fermentation of organic waste |
| CN113897310A (en) * | 2021-09-29 | 2022-01-07 | 中国科学院成都生物研究所 | Bacteria preparation for harmlessly treating dead pigs and using method thereof |
| CN113897310B (en) * | 2021-09-29 | 2023-05-05 | 中国科学院成都生物研究所 | Bacterial preparation for innocent treatment of dead pigs and application method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102068711B (en) | Microbial deodorant as well as preparation method and application thereof | |
| CN102815792B (en) | Microbe-enzyme composite preparation used for restoring water in urban and rural polluted river, and its preparation method | |
| CN101787350B (en) | Functional composite biological agent for high-efficiency degradation of household refuses and application thereof | |
| CN101333510B (en) | Method for processing sludge and preparing bio organic fertilizer and special leaven thereof | |
| CN103509743A (en) | Microbial compound preparation and preparation method | |
| CN105174491B (en) | For deodorant microorganism formulation of water body and its preparation method and application | |
| CN107988099B (en) | Microbial agent for rapid degradation and reduction of organic garbage and application thereof | |
| CN106745828A (en) | A kind of broad-spectrum biological preparation administered for black and odorous water and its application method | |
| CN104152378B (en) | Compound microbial agent for anaerobic digestion treatment of sludge and production method of compound microbial agent | |
| CN102020492A (en) | Efficient wheat straw degradation composite bacteria organic fertilizer and preparation method thereof | |
| CN114574383B (en) | Efficient kitchen waste degradation composite microbial agent and preparation method and application thereof | |
| CN102864107B (en) | Microbial preparation for rehabilitating phenyl amine polluted water body | |
| CN112094782A (en) | Composite microbial inoculum for degrading kitchen garbage and preparation method thereof | |
| CN102102088B (en) | Production method of petroleum hydrocarbon pollutant-degrading bacterium | |
| CN111153717A (en) | Treatment method and strain for on-site treatment and reduction of kitchen waste | |
| CN111593004A (en) | Kitchen waste microbial degradation microbial inoculum and preparation method thereof | |
| CN115125168B (en) | Composite bacterial enzyme agent for kitchen waste treatment and preparation method and application thereof | |
| CN110577905A (en) | Compound microbial agent and preparation method and application thereof | |
| CN103937713B (en) | Microbiological deterioration liquid bacterial agent of food source organic waste and preparation method thereof | |
| CN102925395A (en) | Compound bacterium capable of degrading kitchen waste and preparation method of compound bacterium | |
| CN103305443B (en) | Preparation and method for restoring ammonia nitrogen-containing industrial sewage | |
| CN110591974A (en) | Preparation and use method of thermophilic microbial agent for leather sludge drying | |
| CN113637604B (en) | Microbial inoculum and application thereof in-situ degradation of high-salt high-oil kitchen waste | |
| CN107262495B (en) | A method of refuse odor is administered using composite bacteria agent | |
| CN113846037A (en) | Multi-type organic wet garbage microorganism in-situ hydration microbial inoculum and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220706 Address after: 609, Chuangyan building, No. 355, animation East Road, Zhongxin Tianjin eco city, Binhai New Area, Tianjin 300450 Applicant after: SWANSHINE (TIANJIN) BIOTECHNOLOGY & DEVELOPMENT Ltd. Address before: Room b316-54, building 1, No. 29, life Garden Road, science and Technology Park, Changping District, Beijing Applicant before: Baiaochuangxiang (Beijing) Biotechnology Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |