CN102492621A - Microbe composite bacterial liquid and application thereof in preparing organic fertilizer - Google Patents
Microbe composite bacterial liquid and application thereof in preparing organic fertilizer Download PDFInfo
- Publication number
- CN102492621A CN102492621A CN2011103636265A CN201110363626A CN102492621A CN 102492621 A CN102492621 A CN 102492621A CN 2011103636265 A CN2011103636265 A CN 2011103636265A CN 201110363626 A CN201110363626 A CN 201110363626A CN 102492621 A CN102492621 A CN 102492621A
- Authority
- CN
- China
- Prior art keywords
- fertilizer
- composite
- bacteria liquid
- clostridium
- composite bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.
Description
Technical field
The invention belongs to the microbial fertilizer technical field, be specifically related to a kind of microbial composite bacteria liquid and the application in the preparation fertilizer thereof, promptly a kind of bacterium liquid that is composited by the multiple mutual not mikrobe of antagonism.
Background technology
Mostly China's microbial-bacterial fertilizer is the normal soil useful microbe at present, and mostly most widely used is genus bacillus, includes bacillus megaterium, subtilis and colloid bacillus cereus etc.Bacillus megaterium can be used for the solid potassic fertilizer of production phosphorus decomposing, has the effect of organophosphorus in the good degraded soil; And subtilis mainly should be used for all kinds of fertilizers of fermentation maturity (muck); Colloid bacillus is named silicate bacteria again, and the potassium-bearing mineral that ability analysing silicon glutaric acid salt and aluminosilicate are formed has molten phosphorus, releases potassium and fixed nitrogen function.Thalline can produce the multiple materials that help plant absorbing and utilization such as organic acid, hormone through self metabolism like this, also suppresses other growth of pathogenic bacteria simultaneously.And for the farm crop of establishing in large scale,, then can improve the efficient of farming greatly if bacterial manure can directly efficiently be converted into the meta-bolites that crop can absorb with the Mierocrystalline cellulose of the tillage method of straw-returning, also can reduce the farming cost simultaneously.
Present stalk decomposing microbial inoculum is that cellulose degradation microbial inoculum product is to become thoroughly decomposed mixing of materials such as feces of livestock and poultry or itself and stalk under the condition to banking up and the microbial inoculum product researched and developed mostly, and to the stalk field condition present research of the decomposing microbial inoculum of research and development blank almost down.Therefore, just imperative to the research and development of stalk decomposition agent strain, assembly relation and production unit.
Summary of the invention
The purpose of this invention is to provide a kind of microbial composite bacteria liquid and the application in the preparation fertilizer thereof; I.e. one group of microorganism species and application thereof that degraded cellulose is useful to soil simultaneously; Be can degraded cellulose with itself flora and one group of common micro-organisms flora bacterial manure carry out combination matching, make soil fermentation the becoming high-efficient bacterial fertilizer that is of value to crop growth that it can be high to the unavailable content of cellulose of script plant.
Microbial composite bacteria liquid of the present invention includes the viable bacteria of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri.
The number of viable of above-mentioned bacterium liquid is 25～3,000,000,000/ml;
The substratum of preparation bacterium liquid, the mass percent of its component is following: peptone 0.5%, yeast extract 0.1%, Mierocrystalline cellulose 1%, sodium-chlor 0.5%, lime carbonate 0.5%, the pH of substratum are 8.0.
The quantity ratio of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri is 8: 2: 10 in the above-mentioned composite bacteria liquid: 6: 20: 1: 2.
A kind of bacteria preparation product is the viable bacteria in the above-mentioned composite bacteria liquid to be adsorbed onto on any sorbent material that is used to prepare fertilizer prepare, and described sorbent material is preferably powdered rice hulls.
A kind of method for preparing fertilizer is with above-mentioned bacteria preparation product agricultural crop straw to be fermented.
Complex micro organism fungicide of the present invention can reduce the usage quantity of inorganic composite fertilizer in composite fertilizer's use, reduce production costs.Simultaneously; Under the synergy of microbiobacterial agent; Make composite organic-inorganic fertilizer,, the be rich in nitrogen, phosphorus, potassium, calcium, the organic agriculture and forestry organic waste material stalk of magnesium of lower price is converted into small-molecule substances such as the utilizable monose of plant, oligosaccharides low applying under the condition of nitrogen, phosphorus, potash fertilizer; Thereby obtain higher crop yield, play tangible effect of increasing production.This seriously relies on chemical fertilizer to changing China's agriculture prodn, and strengthening Agro-ecology protection aspect can be with very big help.
Embodiment
The fertilizer efficiency of bio-feritlizer is mainly from the soil useful microbe that wherein contains; The height of useful microbe number of viable has determined quality product and effect, and microorganism live bacteria quantity is determined by the number of viable in the microbiobacterial agent that is adopted in the bio-feritlizer.Simultaneously the height of microorganism live bacteria quantity depends on height and the absorption carrier of number of viable in the zymocyte liquid size to bacterium liquid adsorptive capacity in the microbiobacterial agent.So the height of absorption carrier adsorptive capacity is a main aspect that influences microbial inoculum number of viable and even biological fertilizer products quality.
Composite bacteria liquid of the present invention is selected following mikrobe for use: handkerchief nurse wine clostridium caloritolerans (Clostridium thermopalmarium) can produce cellulase, to the Mierocrystalline cellulose efficient degradation; The thermophilic clostridium of succsinic acid (Clostridium thermosuccinogenes) also is a strain cellulose-degrading bacteria; Potsdam bacillus brevis (Brevibacillus borstelensis) can produce mannase, and glycase etc. have obvious restraining effect to multiple plant-animal pathogenic fungi; Series bacillus (Paenibacillus barengoltzii) can produce like multiple biologically active substances such as microbiotic, antagonist protein, plant hormone, enzyme, flocculation agents.Subtilis (Bacillus subtilis) mainly should be used for all kinds of fertilizers of fermentation maturity (muck); And improve crop disease-resistant, cold-resistant, drought-resistant ability; Increase soil nutrient, the structure of improving the soil, improve chemical fertilizer utilization ratio; Impel the organic matter decomposition in the soil to become soil ulmin, stimulate plant growth.Pseudomonas mendocina (Pseudomonas mendocina) is being brought into play significant feature in the microorganism Decompounding of Pesticide process; Providencia rettgeri (Providencia rettgeri), different oxygen denitrifier promotes the Nitrogen Cycling metabolism.
Below in conjunction with embodiment implementation process of the present invention is done detailed description.
Embodiment 1: the preparation of composite bacteria liquid
1, strain fermentation: with the handkerchief nurse wine clostridium caloritolerans of mixed culture, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus are through shaking table enlarged culturing, seeding tank fermentation and ferment tank, and the bacterial classification ratio is 8: 2: 10: 6.Medium preparation is following: peptone 0.5%, yeast extract 0.1%, Mierocrystalline cellulose (gas explosion stalk) 1%; Sodium-chlor 0.5%, lime carbonate 0.5%, pH 8.0; 50 ℃ leave standstill cultivation, and it is little aerobic between the 0.02-0.4 making its DO, can be with straw degradative more than 50% behind the fermentation 3d.Subtilis, pseudomonas mendocina, three bacterial classifications of providencia rettgeri are fermented and ferment tank through slant culture, shaking table enlarged culturing, seeding tank separately respectively.Its culture medium prescription is following: the nutrient solution after centrifugal removal thalline of above-mentioned mixed bacterial culture 12000g and the impurity sterilization is used for this three kinds of culture of strains, peptone 0.15%, and sodium-chlor 0.5%, 24h is cultivated in 0.05%, 36 ℃ of concussion of Carnis Bovis seu Bubali cream.The number of viable of fermentation back bacterium liquid reaches 25～3,000,000,000/ml.
Embodiment 2: the preparation of bacteria preparation product
Sorbent material of the present invention can be any sorbent material that is used to prepare fertilizer, is preferably powdered rice hulls, and said powdered rice hulls adopts the dry rice husk of processing just to pulverize and forms.The fineness of powdered rice hulls is more than 100 orders, and preferably fineness reaches 60～80 orders.This is because low, the easy pulverizing of powdered rice hulls moisture content; Pulverizing the back fineness can reach more than 100 orders; Its adsorptive capacity exceeds one times in the peat composed of rotten mosses, brown coal and differentiation coal; Complex micro organism fungicide number of viable as absorption carrier production can reach 5～600,000,000/gram, and the peat composed of rotten mosses, brown coal and differentiation coal have only 2～300,000,000/gram as the carrier number of viable, have improved quality product greatly; Because powdered rice hulls is cheap, is used for producing composite microbiological fertilizer and biological organic fertilizer and can reduces the bacterial classification use cost more than 1/3.
The composite bacteria liquid of preparation among the embodiment 1 is adsorbed with powdered rice hulls in the absorption whipping device; Handkerchief nurse wine clostridium caloritolerans with mixed culture; The thermophilic clostridium of succsinic acid; The subtilis of Potsdam bacillus brevis, series bacillus and single culture, pseudomonas mendocina, providencia rettgeri were according to 26: 20: 1: 2 (volumes; L) mix, the quantity ratio of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri is about 8: 2: 10 in the composite bacteria liquid that forms like this: 6: 20: 1: 2.Mixed bacteria liquid and powdered rice hulls with preparation adsorbs then, and absorption proportional volume (L)/quality (kg) is a bacterium liquid: powdered rice hulls=1: 2～4.Stir then, the whipping process with after the powdered rice hulls absorption is not as the criterion with loose the caking.
Embodiment 3: with bacteria preparation product prepn fertilizer
1, sampling
By the volume ratio inoculation of 5% (cultivating the composite bacteria of 3d in advance), 50 ℃ of static cultivations.Handle when carrying out compost as follows: add the composite flora MCl that is selected for use by 1L/T, and add 1 ‰ CaCO3 and be used to regulate pH regulator between yeast phase, inoculation then adds water management stalk water ratio about 55-60% by weight in wet base.Be placed on high, the wide 1.0m of being, carry out static compost in the reaction tank of long 10m.Covered with plastic film. adopt from an end and carry out the oxygen supply of ventilating of turning mode, stop direction to compost every day and turn over and push away one meter distance, and add corresponding stalk and seed liquor to the compost starting position, reaching 55 ℃ with the heap body core temperature is the turning sign.Turning every day once, the temperature of the fertile heap of sequentially determining 10,30,3 levels of 50cm from top to bottom, the compost temperature of the equal temperature of making even as the same day.Compost stalk source: corn straw, rice straw, wheat stalk; Gas explosion treatment condition: 2.4MPa, 3-5min.
Complex micro organism fungicide is effect on cucumber
1), supplies examination soil
Preceding crop: non-irrigated seedling
Soil type: silty loam
Soil analysis result: PH:5.3, organic matter: 13.71g/kg, hydrolyzable nitrogen: 123mg/kg; Available potassium: 82mg/kg, slowly available potassium: 270mg/kg, available phosphorus: 108.8mg/kg; Effective boron: 0.23mg/kg, effective sulphur: 18.45mg/kg, copper: 1.30mg/kg; Zinc: 2.78mg/kg, iron: 116.17mg/kg, manganese: 8.73mg/kg.
2), experimental cultivar: the excellent cucumber in Tianjin
3), experimental technique
3 processing are established in experiment:
Handle one, execute compound organic and inorganic fertilizer 50kg/667m
2
Compound organic and inorganic fertilizer: stalk>=45%, N+P
2O
5+ K
2O>=25%, composite fungus agent>=20%, amino acid>=6%.
Handle two, do not apply fertilizer;
Handle three, common composite fertilizer 50kg/667m
2
Common composite fertilizer: N+P
2O
5+ K2O>=51%
The experiment random alignment, three repetitions, sub-district area 45m
2
The disposable base manure of doing of experiment fertilizer applies.Trench digging 10CM left and right sides earthing.Spraying fertilizer not except that base manure, other level of management are consistent.The program request of digging pit, spacing 33～38cm, sowing is 2 to 3 in each hole.
Randomly draw 15 strains from each sub-district and observe its Other Main Agronomic Characters of record respectively, measure cell production collection period respectively.
4), different treatment is to the influence of cucumber yield
As shown in table 2, the ultimate production of handling a ratio processing three is slightly high, has increased by 2.7%, and difference is not remarkable.Increased by 39.8% than handling two, difference is extremely remarkable.This explanation is used compound organic and inorganic fertilizer and to use high-load inorganic composite fertilizer the same in the plantation of cucumber, can reach good effect of increasing production.
The cucumber yield of table 1 different fertilization
5), different treatment Economic and Efficiency Analysis
As shown in table 2, to compare with processing two, the economic benefit of different treatment all has increase in various degree, and it is the highest wherein to handle an increase.Handle a ratio processing two every mu of net return and increase by 1260 yuan.Handle a ratio processing three every mu of net return and increase by 129 yuan, amplification 2.9%.
The economic benefit of table 2, different fertilization
Embodiment 2: complex micro organism fungicide is to the influence of celery output
1), supplies examination soil
Preceding crop: spinach
Soil type: light loamy texture moisture soil
The soil analysis result: soil layer is deep, and physical features is smooth, and irrigation condition is better, and soil fertility is higher, 0-20cm soil layer organic matter 1.62%, alkali-hydrolyzable nitrogen 79mg/kg, rapid available phosphorus 45.3mg/kg, available potassium 172mg/kg, pH value 7.7.
2), experimental cultivar: texts and pictures draw celery
3), experimental technique
3 processing are established in experiment:
Handle one, execute compound organic and inorganic fertilizer 50kg/667m
2
Compound organic and inorganic fertilizer: stalk>=45%, N+P
2O
5+ K
2O>=25%, composite fungus agent>=20%, amino acid>=6%.
Handle two, do not apply fertilizer;
Handle three, conventional fertilizer+equivalent clear water.
The experiment random alignment, three repetitions, sub-district area 25m
2
The disposable base manure of doing of experiment fertilizer applies.Trench digging 10CM left and right sides earthing.Spraying fertilizer not except that base manure, other level of management are consistent.The sight method for transplanting cultivation of nursing young plants in hothouses is adopted in test.No disease and pest takes place.Before the celery results, celery 35 strains are got in every sub-district, and it is heavy to measure plant height, leaf-area coefficient and individual plant.The disposable results meter in field produces.
4), different treatment is to the influence of celery output
As shown in table 3, handle a ultimate production than processing three and increase, increased by 11.1%, obvious difference.Increased by 28% than handling two, difference is extremely remarkable.This explanation is used compound organic and inorganic fertilizer and to use high-load inorganic composite fertilizer the same in the plantation of celery, can reach effect of increasing production, and effect of increasing production is more remarkable.
The celery output of table 3 different fertilization
5), different treatment Economic and Efficiency Analysis
As shown in table 4, to compare with processing two, the economic benefit of different treatment all has increase in various degree, and it is the highest wherein to handle an increase.Handle a ratio processing two every mu of net return and increase by 2594.9 yuan.Handle a ratio processing three every mu of net return and increase by 1497.8 yuan, amplification 11.6%.
The economic benefit of table 4, different fertilization
Embodiment 3: complex micro organism fungicide is to the influence of rice yield
1), supplies examination soil
Preceding crop: paddy rice
The soil analysis result: organic content is 2.44%, and alkali-hydrolyzable nitrogen is 104mg/kg, and rapid available phosphorus is 67mg/kg, and available potassium is 153mg/kg, pH value 6.5.
2), experimental cultivar: agricultural perfume rice celery
3), experimental technique
3 processing are established in experiment:
Handle one, execute compound organic and inorganic fertilizer 50kg/667m
2
Compound organic and inorganic fertilizer: stalk>=45%, N+P
2O
5+ K
2O>=25%, composite fungus agent>=20%, amino acid>=6%.
Handle two, do not apply fertilizer;
Handle three, conventional fertilizer+equivalent clear water.
The experiment random alignment, three repetitions, sub-district area 75m
2
The disposable base manure of doing of experiment fertilizer applies.Trench digging 10CM left and right sides earthing.Spraying fertilizer not except that base manure, other level of management are consistent.Row cave distance is 30cm * 16cm, 2～3 seedlings/cave.Field irrigation adopts shallow wet dried alternative irrigation method, and the disease pest and weed control is with conventional.
4), different treatment is to the influence of rice yield
As shown in table 5, handle a ultimate production than processing three and increase, increased by 8.6%, difference is more obvious.Increased by 19.2%, significant difference than handling two.This explanation is used compound organic and inorganic fertilizer and to use high-load inorganic composite fertilizer the same in the plantation of paddy rice, can reach effect of increasing production, and effect of increasing production is more remarkable.
The celery output of table 5 different fertilization
5), different treatment Economic and Efficiency Analysis
As shown in table 6, to compare with processing two, the economic benefit of different treatment all has increase in various degree, and it is the highest wherein to handle an increase.Handle a ratio processing two every mu of net return and increase by 322.2 yuan.Handle a ratio processing three every mu of net return and increase by 251 yuan, amplification 12.6%.
The economic benefit of table 6, different fertilization
In sum, the compound organic and inorganic fertilizer that adds complex micro organism fungicide can play tangible effect of increasing production under the synergy of microbiobacterial agent.Compare with inorganic composite fertilizer, it can obtain higher crop yield under low nitrogen, phosphorus, potassium content condition.This seriously relies on chemical fertilizer to changing China's agriculture prodn, and strengthening Agro-ecology protection aspect can be with very big help.
Claims (7)
1. microbial composite bacteria liquid; It is characterized in that described composite bacteria liquid includes the viable bacteria of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri.
2. composite bacteria liquid as claimed in claim 1 is characterized in that number of viable is 25～3,000,000,000/ml in the described composite bacteria liquid.
3. composite bacteria liquid as claimed in claim 1 is characterized in that the quantity ratio of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri in the described composite bacteria liquid is 8: 2: 10: 6: 20: 1: 2.
4. composite bacteria liquid as claimed in claim 1 is characterized in that the substratum that described composite bacteria liquid is used, and the mass percent of its component is following: peptone 0.5%, yeast extract 0.1%, Mierocrystalline cellulose 1%, sodium-chlor 0.5%, lime carbonate 0.5%; The pH of substratum is 8.0.
5. bacteria preparation product is the viable bacteria in the described composite bacteria liquid of claim 1 to be adsorbed onto on any sorbent material that is used to prepare fertilizer prepare.
6. bacteria preparation product as claimed in claim 5 is characterized in that described sorbent material is a powdered rice hulls.
7. a method for preparing fertilizer is with the described bacteria preparation product of claim 6 agricultural crop straw to be fermented.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110363626 CN102492621B (en) | 2011-11-16 | 2011-11-16 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110363626 CN102492621B (en) | 2011-11-16 | 2011-11-16 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102492621A true CN102492621A (en) | 2012-06-13 |
| CN102492621B CN102492621B (en) | 2013-01-23 |
Family
ID=46184484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110363626 Active CN102492621B (en) | 2011-11-16 | 2011-11-16 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102492621B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531583A (en) * | 2014-12-30 | 2015-04-22 | 盐城工学院 | Pseudomonas and microbial flocculant formed by fermenting pseudomonas |
| CN107245465A (en) * | 2017-07-27 | 2017-10-13 | 浙江大学 | A kind of quick composting microbial inoculum and its application suitable for the pure stalk material of high nitrogen |
| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
| CN108410753A (en) * | 2018-02-06 | 2018-08-17 | 安徽师范大学 | A kind of method of composite bacteria agent of degrading straw and preparation method thereof, degrading straw |
| CN110257487A (en) * | 2019-06-27 | 2019-09-20 | 福建省致青生态环保有限公司 | A kind of method and its application calculating microbial inoculum applied amount based on cellulose enzyme gene |
| CN110577905A (en) * | 2019-08-13 | 2019-12-17 | 百奥创想(北京)生物科技有限公司 | Compound microbial agent and preparation method and application thereof |
| CN111548800A (en) * | 2020-05-12 | 2020-08-18 | 山东胜伟盐碱地科技有限公司 | Microbial agent for saline-alkali soil improvement and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1460664A (en) * | 2003-05-19 | 2003-12-10 | 清华大学 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
| CN101892157A (en) * | 2010-08-18 | 2010-11-24 | 孙廷震 | Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof |
-
2011
- 2011-11-16 CN CN 201110363626 patent/CN102492621B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1460664A (en) * | 2003-05-19 | 2003-12-10 | 清华大学 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
| CN101892157A (en) * | 2010-08-18 | 2010-11-24 | 孙廷震 | Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| ALEI GENG 等: "Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and Clostridium thermopalmarium", 《BIORESOURCE TECHNOLOGY》, vol. 101, no. 11, 9 February 2010 (2010-02-09), pages 4029 - 4033, XP 026917439, DOI: doi:10.1016/j.biortech.2010.01.042 * |
| 刘尧 等: "玉米秸秆高效腐解复合菌系CSS-1 的选育及其组成分析", 《中国农业科学》, vol. 43, no. 21, 30 November 2010 (2010-11-30), pages 4437 - 4446 * |
| 刘艳: "嗜热厌氧梭菌降解纤维素及产氢特性研究", 《中国优秀博士学位论文全文数据库》, no. 5, 15 May 2009 (2009-05-15), pages 006 - 42 * |
| 杜然 等: "兼性厌氧复合菌群H纤维素降解和产乙醇能力及生态组成初探", 《生物工程学报》, vol. 26, no. 7, 25 July 2010 (2010-07-25), pages 960 - 965 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531583A (en) * | 2014-12-30 | 2015-04-22 | 盐城工学院 | Pseudomonas and microbial flocculant formed by fermenting pseudomonas |
| CN107245465A (en) * | 2017-07-27 | 2017-10-13 | 浙江大学 | A kind of quick composting microbial inoculum and its application suitable for the pure stalk material of high nitrogen |
| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
| CN108410753A (en) * | 2018-02-06 | 2018-08-17 | 安徽师范大学 | A kind of method of composite bacteria agent of degrading straw and preparation method thereof, degrading straw |
| CN108410753B (en) * | 2018-02-06 | 2021-04-13 | 安徽师范大学 | Composite microbial inoculum for degrading straw, preparation method thereof and method for degrading straw |
| CN110257487A (en) * | 2019-06-27 | 2019-09-20 | 福建省致青生态环保有限公司 | A kind of method and its application calculating microbial inoculum applied amount based on cellulose enzyme gene |
| CN110577905A (en) * | 2019-08-13 | 2019-12-17 | 百奥创想(北京)生物科技有限公司 | Compound microbial agent and preparation method and application thereof |
| CN111548800A (en) * | 2020-05-12 | 2020-08-18 | 山东胜伟盐碱地科技有限公司 | Microbial agent for saline-alkali soil improvement and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102492621B (en) | 2013-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105838644B (en) | Complex micro organism fungicide and bacterial manure and preparation method thereof and the application in reparation salt affected soil | |
| CN105294284B (en) | A kind of preparation method of stalk fermentation bio-feritlizer | |
| CN101759494B (en) | Diseases-prevention organic fertilizer prepared by edible fungi residue | |
| CN103011961B (en) | Active organic bacterial manure and preparation method thereof | |
| CN102992827B (en) | Active organic bacterial fertilizer and preparation method thereof | |
| CN102492621B (en) | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer | |
| CN101724560B (en) | Microbial inoculums and preparation method thereof | |
| CN103011916B (en) | Method for preparing organic bacteria liquid, organic bacteria liquid prepared by method and application of organic bacteria liquid | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN107162816A (en) | A kind of stalk semi-decomposed alkaline land improving fertilizer | |
| CN104789490A (en) | Compound microbial decomposed inocula, and preparation method and application thereof | |
| CN102732459A (en) | Microbial soil conditioner and preparation method thereof | |
| CN101759493B (en) | Biological disease-preventing organic fertilizer and preparation method thereof | |
| CN107473786A (en) | A kind of method that rice straw returning to the field improves soil | |
| CN105985195B (en) | Organic fertilizer and preparation method thereof | |
| CN110305671A (en) | One kind is for facilities vegetable continuous cropping soil biological modification agent and its preparation and application method | |
| CN105330420A (en) | Biological function organic-inorganic compound fertilizer containing amino acid, humic acid and nicotine and preparation method of biological function organic-inorganic compound fertilizer | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field | |
| CN104098402A (en) | Selenium enrichment biological fertilizer | |
| CN106278654A (en) | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage | |
| CN107298633A (en) | A kind of stalk stack retting biological agent | |
| CN104692961A (en) | Microorganism organic material decomposition agent as well as preparation method and applications thereof | |
| CN104774772A (en) | Straw decomposing fungicide with fertility increasing effect and application of fungicide | |
| CN103626532A (en) | Fungicide containing bacillus and application thereof | |
| CN103086801B (en) | Specific liquid fertilizer for beet planting and manufacture method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHI Free format text: FORMER OWNER: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY Effective date: 20140414 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140414 Address after: 300308, No. 32, seven West Road, Tianjin Airport Economic Zone, Dongli, Tianjin Patentee after: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences Address before: 300308, No. 32, seven West Road, Tianjin Airport Economic Zone, Dongli, Tianjin Patentee before: Tianjin Institute of Industrial Biotechnology |