CN102492621B - Microbe composite bacterial liquid and application thereof in preparing organic fertilizer - Google Patents
Microbe composite bacterial liquid and application thereof in preparing organic fertilizer Download PDFInfo
- Publication number
- CN102492621B CN102492621B CN 201110363626 CN201110363626A CN102492621B CN 102492621 B CN102492621 B CN 102492621B CN 201110363626 CN201110363626 CN 201110363626 CN 201110363626 A CN201110363626 A CN 201110363626A CN 102492621 B CN102492621 B CN 102492621B
- Authority
- CN
- China
- Prior art keywords
- composite
- fertilizer
- clostridium
- bacteria
- bacterial liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002131 composite material Substances 0.000 title claims abstract description 37
- 239000007788 liquid Substances 0.000 title claims abstract description 29
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 22
- 239000003895 organic fertilizer Substances 0.000 title abstract description 13
- 239000003337 fertilizer Substances 0.000 claims abstract description 56
- 241000894006 Bacteria Species 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 32
- 238000002360 preparation method Methods 0.000 claims abstract description 22
- 241000588777 Providencia rettgeri Species 0.000 claims abstract description 11
- 241000589755 Pseudomonas mendocina Species 0.000 claims abstract description 11
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 3
- 241000191775 Brevibacillus borstelensis Species 0.000 claims abstract description 3
- 241001147708 Clostridium thermopalmarium Species 0.000 claims abstract description 3
- 241000708785 Paenibacillus barengoltzii Species 0.000 claims abstract description 3
- 241001656792 [Clostridium] thermosuccinogenes Species 0.000 claims abstract description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 18
- 235000009566 rice Nutrition 0.000 claims description 18
- 241000193403 Clostridium Species 0.000 claims description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- 239000001913 cellulose Substances 0.000 claims description 9
- 229920002678 cellulose Polymers 0.000 claims description 9
- 241000193764 Brevibacillus brevis Species 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 8
- 239000010902 straw Substances 0.000 claims description 8
- 239000002594 sorbent Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 26
- 238000004519 manufacturing process Methods 0.000 abstract description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 14
- 230000001965 increasing effect Effects 0.000 abstract description 14
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 10
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 10
- 229910052700 potassium Inorganic materials 0.000 abstract description 10
- 239000011591 potassium Substances 0.000 abstract description 10
- 239000000126 substance Substances 0.000 abstract description 7
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052791 calcium Inorganic materials 0.000 abstract description 2
- 239000011575 calcium Substances 0.000 abstract description 2
- 229920001542 oligosaccharide Polymers 0.000 abstract description 2
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 2
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 abstract 2
- 150000002772 monosaccharides Chemical class 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 238000012545 processing Methods 0.000 description 20
- 239000002689 soil Substances 0.000 description 18
- 241000209094 Oryza Species 0.000 description 17
- 244000005700 microbiome Species 0.000 description 15
- 150000001875 compounds Chemical class 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 240000007087 Apium graveolens Species 0.000 description 9
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 9
- 235000010591 Appio Nutrition 0.000 description 9
- 210000003608 fece Anatomy 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 239000010871 livestock manure Substances 0.000 description 8
- 239000011574 phosphorus Substances 0.000 description 8
- 229910052698 phosphorus Inorganic materials 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 239000002361 compost Substances 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 6
- 230000000855 fungicidal effect Effects 0.000 description 6
- 239000000417 fungicide Substances 0.000 description 6
- 240000008067 Cucumis sativus Species 0.000 description 5
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 239000002068 microbial inoculum Substances 0.000 description 5
- 241000233866 Fungi Species 0.000 description 3
- 230000000274 adsorptive effect Effects 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 235000010216 calcium carbonate Nutrition 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000003973 irrigation Methods 0.000 description 3
- 230000002262 irrigation Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 238000004856 soil analysis Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 2
- 241000195940 Bryophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003245 coal Substances 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000004880 explosion Methods 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000003077 lignite Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000003415 peat Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 102100032487 Beta-mannosidase Human genes 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- DPOUJDUNLZOBCZ-UHFFFAOYSA-J C(CCCC(=O)[O-])(=O)[O-].[Si+4].C(CCCC(=O)[O-])(=O)[O-] Chemical compound C(CCCC(=O)[O-])(=O)[O-].[Si+4].C(CCCC(=O)[O-])(=O)[O-] DPOUJDUNLZOBCZ-UHFFFAOYSA-J 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 108010055059 beta-Mannosidase Proteins 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical group O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.
Description
Technical field
The invention belongs to the microbial fertilizer technical field, be specifically related to a kind of microbe composite bacterial liquid and in the application of preparation in the fertilizer, namely a kind of by the multiple mutually bacterium liquid that is composited of the microorganism of antagonism not, and its preparation method and application.
Background technology
At present China's microbial-bacterial fertilizer mostly is the normal soil beneficial microorganism, most widely usedly mostly is genus bacillus, includes bacillus megaterium, subtilis and colloid bacillus cereus etc.Bacillus megaterium can be used for the solid potassic fertilizer of production phosphorus decomposing, has the effect of organophosphorus in the good degraded soil; And subtilis mainly should be used for all kinds of fertilizers of fermentation maturity (muck); Colloid bacillus is named again silicate bacteria, and the potassium-bearing mineral that energy analysing silicon glutaric acid salt and aluminosilicate form has molten phosphorus, releases potassium and fixed nitrogen function.Thalline can produce the multiple materials that are conducive to plant absorbing and utilization such as organic acid, hormone by self metabolism like this, also suppresses the growth of other pathogenic bacteria simultaneously.And for the farm crop of establishing in large scale, if bacterial manure can be the meta-bolites that crop can absorb with the direct Efficient Conversion of Mierocrystalline cellulose of the tillage method of straw-returning, then can greatly improve the efficient of farming, also can reduce the farming cost simultaneously.
Present straw decomposing microbial inoculum is that cellulose degradation microbial inoculum product is the microbial inoculum product of researching and developing for the mixing of materials such as become thoroughly decomposed under the condition of banking up feces of livestock and poultry or itself and stalk mostly, and almost blank for the present research of the decomposing microbial inoculum of researching and developing under the stalk field condition.Therefore, just imperative to the research and development of straw decomposing inoculant bacterial classification, assembly relation and production unit.
Summary of the invention
The purpose of this invention is to provide a kind of microbe composite bacterial liquid and the application in the preparation fertilizer thereof, i.e. simultaneously microorganism species and the application thereof useful to soil of one group of degraded cellulose, be can degraded cellulose with itself flora and one group of common micro-organisms flora bacterial manure carry out combination matching, can become the high-efficient bacterial fertilizer that is of value to crop growth to the high soil fermentation of the unavailable content of cellulose of script plant.
Microbe composite bacterial liquid of the present invention includes the viable bacteria of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri.
The number of viable of above-mentioned bacterium liquid is 25～3,000,000,000/ml;
The substratum of preparation bacterium liquid, the mass percent of its component is as follows: peptone 0.5%, yeast extract 0.1%, Mierocrystalline cellulose 1%, sodium-chlor 0.5%, calcium carbonate 0.5%, the pH of substratum are 8.0.
The quantity ratio of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri is 8: 2: 10 in the above-mentioned composite bacteria liquid: 6: 20: 1: 2.
A kind of bacteria preparation product is the viable bacteria in the above-mentioned composite bacteria liquid to be adsorbed onto on any sorbent material for the preparation of fertilizer prepare, and described sorbent material is preferably powdered rice hulls.
A kind of method for preparing fertilizer is with above-mentioned bacteria preparation product agricultural crop straw to be fermented.
Complex micro organism fungicide of the present invention can reduce the usage quantity of inorganic composite fertilizer in composite fertilizer's use procedure, reduce production costs.Simultaneously, under the synergy of microbiobacterial agent, make composite organic-inorganic fertilizer, low applying under the condition of nitrogen, phosphorus, potash fertilizer, the agriculture and forestry organic waste material stalk that is rich in nitrogen, phosphorus, potassium, calcium, magnesium organic matter of lower price is converted into the small-molecule substances such as the utilizable monose of plant, oligosaccharides, thereby obtain higher crop yield, play obvious effect of increasing production.This seriously relies on chemical fertilizer to changing China's agriculture production, strengthens Agro-ecology protection aspect and can use very large help.
Embodiment
The fertilizer efficiency of bio-feritlizer is mainly from the soil beneficial microorganism that wherein contains, the height of beneficial microorganism number of viable has determined quality product and effect, and microorganism live bacteria quantity is determined by the number of viable in the microbiobacterial agent that adopts in the bio-feritlizer.Simultaneously the height of microorganism live bacteria quantity depends on that the height of number of viable in the zymocyte liquid and absorption carrier are to the size of bacterium liquid adsorptive capacity in the microbiobacterial agent.So the height of absorption carrier adsorptive capacity is a main aspect that affects microbial inoculum number of viable and even biological fertilizer products quality.
Composite bacteria liquid of the present invention is selected following microorganism: handkerchief nurse wine clostridium caloritolerans (Clostridium thermopalmarium) can produce cellulase, to the Mierocrystalline cellulose efficient degradation; The thermophilic clostridium of succsinic acid (Clostridium thermosuccinogenes) also is a strain cellulose-degrading bacteria; Potsdam bacillus brevis (Brevibacillus borstelensis) can produce mannase, and amylase etc. have obvious restraining effect to multiple animals and plants pathogenic fungi; Series bacillus (Paenibacillus barengoltzii) can produce such as various bioactivators such as microbiotic, antagonist protein, plant hormone, enzyme, flocculation agents.Subtilis (Bacillus subtilis) mainly should be used for all kinds of fertilizers of fermentation maturity (muck); And improve crop disease-resistant, cold-resistant, drought-resistant ability; Increase soil nutrient, improvement Soil structure, improve chemical fertilizer utilization ratio; Impel the organic matter decomposition in the soil to become soil ulmin, stimulate plant growth.Pseudomonas mendocina (Pseudomonas mendocina) is being brought into play main effect in the microbiological deterioration process of agricultural chemicals; Providencia rettgeri (Providencia rettgeri), different oxygen denitrifier promotes the Nitrogen Cycling metabolism.
Below in conjunction with embodiment the invention process process is done detailed description.
Embodiment 1: the preparation of composite bacteria liquid
1, strain fermentation: with the handkerchief nurse wine clostridium caloritolerans of mixed culture, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus are through shaking table enlarged culturing, seeding tank fermentation and ferment tank, and the bacterial classification ratio is 8: 2: 10: 6.Substratum is prepared as follows: peptone 0.5%, yeast extract 0.1%, Mierocrystalline cellulose (gas explosion stalk) 1%, sodium-chlor 0.5%, calcium carbonate 0.5%, pH 8.0,50 ℃ leave standstill cultivation, and it is little aerobic between the 0.02-0.4 making its DO, can be with straw degradative more than 50% behind the fermentation 3d.Subtilis, pseudomonas mendocina, three bacterial classifications of providencia rettgeri are fermented and ferment tank through slant culture, shaking table enlarged culturing, seeding tank separately respectively.Its culture medium prescription is as follows: the nutrient solution after the centrifugal removal thalline of above-mentioned mixed bacterial culture 12000g and the impurity sterilization is used for the cultivation of these three kinds of bacterial classifications, peptone 0.15%, and sodium-chlor 0.5%, 24h is cultivated in 0.05%, 36 ℃ of concussion of extractum carnis.The number of viable of bacterium liquid reaches 25～3,000,000,000/ml after the fermentation.
Embodiment 2: the preparation of bacteria preparation product
Sorbent material of the present invention can be any for the preparation of the sorbent material of fertilizer, be preferably powdered rice hulls, described powdered rice hulls adopts the dry rice husk of just processing to pulverize and forms.The fineness of powdered rice hulls is more than 100 orders, and preferably fineness reaches 60～80 orders.This is because low, the easy pulverizing of powdered rice hulls moisture content, fineness can reach more than 100 orders after pulverizing, its adsorptive capacity exceeds one times in the peat composed of rotten mosses, brown coal and differentiation coal, complex micro organism fungicide number of viable as absorption carrier production can reach 5～600,000,000/gram, and the peat composed of rotten mosses, brown coal and differentiation coal only have 2～300,000,000/gram as the carrier number of viable, have greatly improved quality product; Because powdered rice hulls is cheap, is used for producing composite microbiological fertilizer and biological organic fertilizer and can reduces the bacterial classification use cost more than 1/3.
The composite bacteria liquid of preparation among the embodiment 1 is adsorbed with powdered rice hulls in the absorption whipping device, handkerchief nurse wine clostridium caloritolerans with mixed culture, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, the subtilis of series bacillus and single culture, pseudomonas mendocina, providencia rettgeri was according to 26: 20: 1: 2 (volumes, L) mix handkerchief nurse wine clostridium caloritolerans in the composite bacteria liquid that forms like this, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, the quantity ratio of pseudomonas mendocina and providencia rettgeri is about 8: 2: 10: 6: 20: 1: 2.Then mixed bacteria liquid and the powdered rice hulls with preparation adsorbs, and ratio of adsorption volume (L)/quality (kg) is bacterium liquid: powdered rice hulls=1: 2～4.Then stir, the whipping process with after the powdered rice hulls absorption is not as the criterion with loose the caking.
Embodiment 3: prepare fertilizer with the bacteria preparation product
1, sampling
By the volume ratio inoculation of 5% (composite bacteria of preculture 3d), 50 ℃ of static cultivations.Process as follows when carrying out compost: press 1L/T and add selected composite flora MCl, and add 1 ‰ CaCO3 for regulating pH regulator between yeast phase, add the water management moisture content of straws about 55-60% by weight in wet base after the inoculation.Be placed on high, the wide 1.0m of being, carry out static compost in the reaction tank of long 10m.Covered with plastic film. adopt from an end and carry out the oxygen supply of ventilating of turning mode, stop direction to compost every day and turn over and push away one meter distance, and add corresponding stalk and seed liquor to the compost starting position, reach 55 ℃ as the turning sign take the heap body core temperature.Turning every day once, the temperature of the fertile heap 10,30 of sequentially determining, 3 levels of 50cm is averaged the compost temperature of temperature as the same day from top to bottom.Compost stalk source: maize straw, rice straw, wheat stalk; Gas explosion treatment condition: 2.4MPa, 3-5min.
Complex micro organism fungicide is effect on cucumber
1), for examination soil
Preceding crop: non-irrigated seedling
Soil type: silty loam
Soil analysis result: PH:5.3, organic: 13.71g/kg, hydrolyzable nitrogen: 123mg/kg, available potassium: 82mg/kg, slowly available potassium: 270mg/kg, available phosphorus: 108.8mg/kg, effectively boron: 0.23mg/kg, effectively sulphur: 18.45mg/kg, copper: 1.30mg/kg, zinc: 2.78mg/kg, iron: 116.17mg/kg, manganese: 8.73mg/kg.
2), experimental cultivar: the excellent cucumber in Tianjin
3), experimental technique
3 processing are established in experiment:
Process one, execute compound organic and inorganic fertilizer 50kg/667m
2
Compound organic and inorganic fertilizer: stalk 〉=45%, N+P
2O
5+ K
2O 〉=25%, composite fungus agent 〉=20%, amino acid 〉=6%.
Process two, do not apply fertilizer;
Process three, Common compound fertilizer 50kg/667m
2
Common compound fertilizer: N+P
2O
5+ K2O 〉=51%
The experiment random alignment, three repetitions, residential quarter area 45m
2
The disposable base manure of doing of experiment fertilizer applies.Trench digging 10CM left and right sides earthing.Spraying fertilizer not except base manure, other level of management are consistent.The program request of digging pit, spacing 33～38cm, sowing is 2 to 3 in each hole.
Randomly draw 15 strains from each residential quarter and observe respectively its Other Main Agronomic Characters of record, measure respectively cell production collection period.
4), different treatment is on the impact of cucumber yield
As shown in table 2, the ultimate production of processing a ratio processing three is slightly high, has increased by 2.7%, and difference is not remarkable.Increased by 39.8% than processing two, difference is extremely remarkable.This explanation is in the plantation of cucumber, and it is the same with the inorganic composite fertilizer of using high-content to use compound organic and inorganic fertilizer, can reach good effect of increasing production.
The cucumber yield of table 1 different fertilization
5), different treatment Economic and Efficiency Analysis
As shown in table 2, to compare with processing two, the economic benefit of different treatment has increase in various degree, and it is the highest wherein to process an increase.Process a ratio processing two every mu of net return and increase by 1260 yuan.Process a ratio processing three every mu of net return and increase by 129 yuan, amplification 2.9%.
The economic benefit of table 2, different fertilization
Embodiment 2: complex micro organism fungicide is on the impact of celery output
1), for examination soil
Preceding crop: spinach
Soil type: light loamy soil
The soil analysis result: soil layer is deep, and physical features is smooth, and irrigation condition is better, and soil fertility is higher, 0-20cm soil layer organic matter 1.62%, alkali-hydrolyzable nitrogen 79mg/kg, rapid available phosphorus 45.3mg/kg, available potassium 172mg/kg, pH value 7.7.
2), experimental cultivar: texts and pictures draw celery
3), experimental technique
3 processing are established in experiment:
Process one, execute compound organic and inorganic fertilizer 50kg/667m
2
Compound organic and inorganic fertilizer: stalk 〉=45%, N+P
2O
5+ K
2O 〉=25%, composite fungus agent 〉=20%, amino acid 〉=6%.
Process two, do not apply fertilizer;
Process three, conventional fertilizer+equivalent clear water.
The experiment random alignment, three repetitions, residential quarter area 25m
2
The disposable base manure of doing of experiment fertilizer applies.Trench digging 10CM left and right sides earthing.Spraying fertilizer not except base manure, other level of management are consistent.The sight method for transplanting cultivation of nursing young plants in hothouses is adopted in test.Occur without disease and pest.Before the celery results, celery 35 strains are got in every residential quarter, and it is heavy to measure plant height, leaf-area coefficient and individual plant.The disposable results meter in field produces.
4), different treatment is on the impact of celery output
As shown in table 3, process a ultimate production than processing three and increase, increased by 11.1%, obvious difference.Increased by 28% than processing two, difference is extremely remarkable.This explanation is in the plantation of celery, and it is the same with the inorganic composite fertilizer of using high-content to use compound organic and inorganic fertilizer, can reach effect of increasing production, and effect of increasing production is more remarkable.
The celery output of table 3 different fertilization
5), different treatment Economic and Efficiency Analysis
As shown in table 4, to compare with processing two, the economic benefit of different treatment has increase in various degree, and it is the highest wherein to process an increase.Process a ratio processing two every mu of net return and increase by 2594.9 yuan.Process a ratio processing three every mu of net return and increase by 1497.8 yuan, amplification 11.6%.
The economic benefit of table 4, different fertilization
Embodiment 3: complex micro organism fungicide is on the impact of rice yield
1), for examination soil
Preceding crop: paddy rice
The soil analysis result: organic content is 2.44%, and alkali-hydrolyzable nitrogen is 104mg/kg, and rapid available phosphorus is 67mg/kg, and available potassium is 153mg/kg, pH value 6.5.
2), experimental cultivar: agricultural perfume rice celery
3), experimental technique
3 processing are established in experiment:
Process one, execute compound organic and inorganic fertilizer 50kg/667m
2
Compound organic and inorganic fertilizer: stalk 〉=45%, N+P
2O
5+ K
2O 〉=25%, composite fungus agent 〉=20%, amino acid 〉=6%.
Process two, do not apply fertilizer;
Process three, conventional fertilizer+equivalent clear water.
The experiment random alignment, three repetitions, residential quarter area 75m
2
The disposable base manure of doing of experiment fertilizer applies.Trench digging 10CM left and right sides earthing.Spraying fertilizer not except base manure, other level of management are consistent.Row cave distance is 30cm * 16cm, 2～3 seedlings/cave.Field irrigation adopts shallow wet dried alternative irrigation method, and the disease pest and weed control is with conventional.
4), different treatment is on the impact of rice yield
As shown in table 5, process a ultimate production than processing three and increase, increased by 8.6%, difference is more obvious.Increased by 19.2%, significant difference than processing two.This explanation is in the plantation of paddy rice, and it is the same with the inorganic composite fertilizer of using high-content to use compound organic and inorganic fertilizer, can reach effect of increasing production, and effect of increasing production is more remarkable.
The celery output of table 5 different fertilization
5), different treatment Economic and Efficiency Analysis
As shown in table 6, to compare with processing two, the economic benefit of different treatment has increase in various degree, and it is the highest wherein to process an increase.Process a ratio processing two every mu of net return and increase by 322.2 yuan.Process a ratio processing three every mu of net return and increase by 251 yuan, amplification 12.6%.
The economic benefit of table 6, different fertilization
In sum, add the compound organic and inorganic fertilizer of complex micro organism fungicide under the synergy of microbiobacterial agent, can play obvious effect of increasing production.Compare with inorganic composite fertilizer, it can obtain higher crop yield under low nitrogen, phosphorus, potassium content condition.This seriously relies on chemical fertilizer to changing China's agriculture production, strengthens Agro-ecology protection aspect and can use very large help.
Claims (5)
1. microbe composite bacterial liquid, it is characterized in that described composite bacteria liquid is by handkerchief nurse wine clostridium caloritolerans (Clostridium thermopalmarium), the thermophilic clostridium of succsinic acid (Clostridium thermosuccinogenes), Potsdam bacillus brevis (Brevibacillus borstelensis), series bacillus (Paenibacillus barengoltzii), subtilis (Bacillus subtilis), the viable bacteria of pseudomonas mendocina (Pseudomonas mendocina) and providencia rettgeri (Providencia rettgeri) forms; Number of viable is 25～3,000,000,000/ml in the composite bacteria liquid, and the quantity of handkerchief nurse wine clostridium caloritolerans, the thermophilic clostridium of succsinic acid, Potsdam bacillus brevis, series bacillus, subtilis, pseudomonas mendocina and providencia rettgeri is than being 8:2:10:6:20:1:2.
2. composite bacteria liquid as claimed in claim 1 is characterized in that the substratum that described composite bacteria liquid is used, and the mass percent of its component is as follows: peptone 0.5%, yeast extract 0.1%, Mierocrystalline cellulose 1%, sodium-chlor 0.5%, calcium carbonate 0.5%; The pH of substratum is 8.0.
3. a bacteria preparation product is the viable bacteria in the composite bacteria liquid claimed in claim 1 to be adsorbed onto on any sorbent material for the preparation of fertilizer prepare.
4. bacteria preparation product as claimed in claim 3 is characterized in that described sorbent material is powdered rice hulls.
5. a method for preparing fertilizer is with bacteria preparation product claimed in claim 4 agricultural crop straw to be fermented.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110363626 CN102492621B (en) | 2011-11-16 | 2011-11-16 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110363626 CN102492621B (en) | 2011-11-16 | 2011-11-16 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102492621A CN102492621A (en) | 2012-06-13 |
| CN102492621B true CN102492621B (en) | 2013-01-23 |
Family
ID=46184484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110363626 Expired - Fee Related CN102492621B (en) | 2011-11-16 | 2011-11-16 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102492621B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531583B (en) * | 2014-12-30 | 2017-05-31 | 盐城工学院 | A kind of pseudomonad and the microbial flocculant obtained in it ferments |
| CN107245465A (en) * | 2017-07-27 | 2017-10-13 | 浙江大学 | A kind of quick composting microbial inoculum and its application suitable for the pure stalk material of high nitrogen |
| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
| CN108410753B (en) * | 2018-02-06 | 2021-04-13 | 安徽师范大学 | Composite microbial inoculum for degrading straw, preparation method thereof and method for degrading straw |
| CN110257487A (en) * | 2019-06-27 | 2019-09-20 | 福建省致青生态环保有限公司 | A kind of method and its application calculating microbial inoculum applied amount based on cellulose enzyme gene |
| CN110577905A (en) * | 2019-08-13 | 2019-12-17 | 百奥创想(北京)生物科技有限公司 | Compound microbial agent and preparation method and application thereof |
| CN111548800A (en) * | 2020-05-12 | 2020-08-18 | 山东胜伟盐碱地科技有限公司 | Microbial agent for saline-alkali soil improvement and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1460664A (en) * | 2003-05-19 | 2003-12-10 | 清华大学 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
| CN101892157A (en) * | 2010-08-18 | 2010-11-24 | 孙廷震 | Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof |
-
2011
- 2011-11-16 CN CN 201110363626 patent/CN102492621B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1460664A (en) * | 2003-05-19 | 2003-12-10 | 清华大学 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
| CN101892157A (en) * | 2010-08-18 | 2010-11-24 | 孙廷震 | Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof |
Non-Patent Citations (9)
| Title |
|---|
| Alei Geng 等.Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and Clostridium thermopalmarium.《Bioresource Technology》.2010,第101卷(第11期),4029-4033. |
| CNC12N1/20A 2007.10.17 |
| Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and Clostridium thermopalmarium;Alei Geng 等;《Bioresource Technology》;20100209;第101卷(第11期);4029-4033 * |
| 兼性厌氧复合菌群H纤维素降解和产乙醇能力及生态组成初探;杜然 等;《生物工程学报》;20100725;第26卷(第7期);960-965 * |
| 刘尧 等.玉米秸秆高效腐解复合菌系CSS-1 的选育及其组成分析.《中国农业科学》.2010,第43卷(第21期),4437-4446. |
| 刘艳.嗜热厌氧梭菌降解纤维素及产氢特性研究.《中国优秀博士学位论文全文数据库》.2009,(第5期),A006-42. |
| 嗜热厌氧梭菌降解纤维素及产氢特性研究;刘艳;《中国优秀博士学位论文全文数据库》;20090515(第5期);A006-42 * |
| 杜然 等.兼性厌氧复合菌群H纤维素降解和产乙醇能力及生态组成初探.《生物工程学报》.2010,第26卷(第7期),960-965. |
| 玉米秸秆高效腐解复合菌系CSS-1 的选育及其组成分析;刘尧 等;《中国农业科学》;20101130;第43卷(第21期);4437-4446 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102492621A (en) | 2012-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105838644B (en) | Complex micro organism fungicide and bacterial manure and preparation method thereof and the application in reparation salt affected soil | |
| CN105294284B (en) | A kind of preparation method of stalk fermentation bio-feritlizer | |
| CN102492621B (en) | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer | |
| CN103848698B (en) | A kind of biological organic fertilizer utilizing natural pond slag aerobic fermentation to prepare and preparation method thereof | |
| CN103011961B (en) | Active organic bacterial manure and preparation method thereof | |
| CN102992827B (en) | Active organic bacterial fertilizer and preparation method thereof | |
| CN101724560B (en) | Microbial inoculums and preparation method thereof | |
| CN103011916B (en) | Method for preparing organic bacteria liquid, organic bacteria liquid prepared by method and application of organic bacteria liquid | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN104030751A (en) | Bioorganic compound fertilizer and preparation method thereof | |
| CN108503466A (en) | A kind of tea tree special bio bacterial manure and the preparation method and application thereof | |
| CN102732459A (en) | Microbial soil conditioner and preparation method thereof | |
| CN109762765B (en) | Decomposed solid fermentation microbial inoculum and application thereof in agricultural wastes | |
| CN104789490A (en) | Compound microbial decomposed inocula, and preparation method and application thereof | |
| CN107473786A (en) | A kind of method that rice straw returning to the field improves soil | |
| CN105330420A (en) | Biological function organic-inorganic compound fertilizer containing amino acid, humic acid and nicotine and preparation method of biological function organic-inorganic compound fertilizer | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field | |
| CN104098402A (en) | Selenium enrichment biological fertilizer | |
| CN107298633A (en) | A kind of stalk stack retting biological agent | |
| CN106278654A (en) | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage | |
| CN104774772A (en) | Straw decomposing fungicide with fertility increasing effect and application of fungicide | |
| CN108863462A (en) | It is a kind of using stalk as biological organic fertilizer of raw material and preparation method thereof | |
| CN104496573A (en) | Special biocontrol compound microbial fertilizer for grapes and production method and application | |
| CN105985195A (en) | Organic liquid fertilizer and preparation method thereof | |
| CN104692969A (en) | Biological humic acid liquid fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHI Free format text: FORMER OWNER: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY Effective date: 20140414 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140414 Address after: 300308, No. 32, seven West Road, Tianjin Airport Economic Zone, Dongli, Tianjin Patentee after: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES Address before: 300308, No. 32, seven West Road, Tianjin Airport Economic Zone, Dongli, Tianjin Patentee before: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130123 |