CN1460664A - Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation - Google Patents
Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation Download PDFInfo
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- CN1460664A CN1460664A CN03136613A CN03136613A CN1460664A CN 1460664 A CN1460664 A CN 1460664A CN 03136613 A CN03136613 A CN 03136613A CN 03136613 A CN03136613 A CN 03136613A CN 1460664 A CN1460664 A CN 1460664A
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- composite fungus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention discloses a lignocellulose solid waste composting decomposition-promoting composite bacterial preparation used in the aerobic composting process of the agricultural solid wastes and urban garden wastes. Said bacterial preparation includes 1# and 2# which are cultured by means of fermentation in sugar-added soybean milk as liquid fermentation culture medium or in solid fermentation culture medium made of sawdust and rice bran, and respectively can be used in first fermentation high-temp. stage and secondary fermentation cooling stage for making lignocellulose produce degrading capacity and accelerating mineralization and humidification process of intemediate product.
Description
Technical field
The invention belongs to microbial fermentation microbial inoculum scope, particularly be used to quicken the short rotten composite fungus agent of a kind of lignocellulose solid waste composting of the aerobic compost treating processes of the high agricultural solid waste of wood fibre cellulose content, city trees and shrubs refuse.
Background technology
The development work of compost composite fungus agent has early been carried out in western developed country and China Taiwan, but main fermenting additive and composite fertilizer's microbial inoculum based on fowl and animal excrement.In recent years, the product development of domestic refuse microbiological deterioration microbial inoculum becomes focus in developed country, and some product has been realized commercialization, still, for handling China's agricultural solid waste and municipal wastes, the suitability of these products and effect await further to study and checking.Especially agricultural solid waste big for output, that composition is complicated, the wood fibre cellulose content is high and urban landscaping refuse, its recycling needs special-purpose high-effective microorganism Inoculant, and China relatively lags behind for environment-friendly microbial Research of Microorganisms Agent and application, also is being in the starting stage aspect the research of solid waste microbiological treatment.
Aerobic compost is the important means of agricultural solid waste, urban landscaping refuse and domestic garbage resource and harmless treatment.Composting technology is that organic solid waste is mixed according to a certain percentage with stopping composition, under the condition of suitable moisture, pH, oxygen, carbon-nitrogen ratio, make microbial reproduction and the organic matter of degrading, and produce high temperature, kill wherein pathogenic bacteria and weed seed, make organism reach the process of stabilization.In aerobic composting process, only rely on original indigenous microorganism in the compost substrate to be difficult to decompose effectively in the compost material the slowly organic composition of degraded such as lignocellulose, need to introduce specific inoculating microbe, to quicken the digest process of compost, coordinate the degradation process of different degradation capability materials.In the compost maturity process, cellulosic decomposition is crucial: (1) Mierocrystalline cellulose is the quantity maximum, accounts for the 40%-60% of plant dry matter, and decomposes nonnitrogenous slowly organic substance; (2) cellulosic destruction means the disintegration of cellular material and the generation of incorporeity material, and this is the most important physical behavior variation in the compost maturity process.Therefore, the plain decomposition techniques of various accelerating fiberses that comprise artificial inoculation all have positive effect.But so far, aerobic compost microbial inoculant Research of Microorganisms Agent still is confined to the experimental phase, is not applied to scale operation.Following 2 points of tracing it to its cause: 1, aerobic composting process is the microbial reaction of a complexity, wherein relate to the participation of various bacteria, actinomycetes and fungi, dynamic process for reaction does not also have perfect theoretical basis, is difficult to whole process is artificially controlled; 2, for the compost Inoculant, especially the expansion production technique of cellulose degradation microbial inoculum does not also have intensive research, can't the mass production Inoculant.And the microbial inoculant that carries out extensive compost production needs a large amount of microbiobacterial agents, the cultivation of these microbial inoculums is current to be as selective medium with Congo red substratum, Mierocrystalline cellulose filter paper bar fermention medium or LB substratum are as proliferated culture medium, and filter paper bar substratum is cultivated as detecting substratum.The microbial inoculum that only relies on the laboratory enrichment culture and obtain is difficult to satisfy the production needs.
Summary of the invention
The purpose of this invention is to provide the short rotten composite fungus agent of a kind of lignocellulose solid waste composting.It is characterized in that: described composite fungus agent comprises the microbial inoculum of composite fungus agent 1# and two models of 2#, is applicable to thermophilic fermentation stage (one time fermentation) and normal temperature/cooling fermentation stage (Secondary Fermentation) in the composting process respectively.Wherein the 1# composite fungus agent is made up of the thermophilic ligocellulose degradation of 4-8 strain bacterium, can tolerate 70 ℃-90 ℃ high temperature, and at high temperature brings into play the degradation capability of lignocellulose; The 2# composite fungus agent is had a liking for warm ligocellulose degradation bacterium and 4-8 strain by the 8-12 strain and is had a liking for warm ligocellulose degradation fungi and form, and can quicken intermediate product mineralising and humification process 50 ℃-15 ℃ middle gentle normal temperature stage performance ligocellulose degradation ability.The compost treatment that is used for afforestation refuses such as agricultural solid wastes such as vegetables refuse, flower straw and dry branches and fallen leaves, beta pruning weeds, by accelerating the degraded and the conversion of lignocellulose, degradation process between collaborative different degradation capability refuses, reach the shortening fermentation time, promote compost maturity, improve quality of compost products.
The cultivation of described composite fungus agent is as the liquid state fermentation substratum or use sawdust with the sugaring soya-bean milk, materials such as rice bran are as solid-state fermentation culture medium, bulk fermentation production at short notice, wherein the culture medium prescription of liquid state fermentation is new fresh soya-bean milk 10-50ml, glucose 20g, add water to 1L, pH 6-8, the composite fungus agent inoculum size with 1-20% in this substratum can reach maximum harvest yield 10 in foster 40-70 hour 25 ℃-35 ℃ continuously sudden and violent down air cultures
9/ ml; And be sawdust 80%, rice bran 10%, wheat bran 10%, (NH at prescription
4)
2SO
41.5%, KH
2PO
40.5%, in the solid-state fermentation culture medium of water ratio 50%-60%, this composite fungus agent is cultivated with inoculum size turning at intermittence under 25 ℃-50 ℃ of 1-20% can reach maximum harvest yield 10 in 2 weeks
9/ g, production concentration all reach required standard.The physical form of its corresponding microbial inoculum product is: liquid microbial inoculum is the beige suspension liquid, and pH is 8.0-8.5; Solid-state microbial inoculum is loose pale brown look sawdust form; Bacterial classification content in the product: liquid microbial inoculum bacteria containing amount in 12 months quality guaranteed perioves＞10
9/ ml, solid-state microbial inoculum＞10
9/ g.
The invention has the beneficial effects as follows 1. by using composite fungus agent 1#,, promote the bio-transformation of the lignocellulose in this stage to improve the population density of lignocellulose decomposer in the pliotherm period compost at hot stage.And use composite fungus agent 2# in cooling and steady stage, have a liking for warm lignocellulose decomposer population density with the follow-up property degraded of the lignocellulose in enhancing normal temperature stage and to the mineralising and the humify of middle product by increase.2. contained Mierocrystalline cellulose of this product and the lignin degradation composite fungus agent minimizing and the recycling treatment that can be used for high agricultural solid waste of wood fibre cellulose content and afforestation refuse.When handling the garden refuse, composting plant, destructor plant or individual use this product, two groups of composite fungus agents are used and can improve warm 2-4 ℃ in heap than other biological and abiotic control treatment, the hot stage time length prolongs, strengthened the activity of overall micro-flora and ligocellulose degradation bacterium in the heap body, obviously quicken the associating compost process of vegetable or flower stalk, shorten and to become thoroughly decomposed the time, and (yield of grain diameter＜1cm) improves more than 30% to make final meticulous composting production.
Embodiment
The present invention is the short rotten composite fungus agent of a kind of lignocellulose solid waste composting.It is characterized in that: described composite fungus agent comprises the microbial inoculum of composite fungus agent 1# and two models of 2#, is applicable to thermophilic fermentation stage (one time fermentation) and normal temperature/cooling fermentation stage (Secondary Fermentation) in the composting process respectively.Wherein the 1# composite fungus agent is made up of the thermophilic ligocellulose degradation of 5 strains bacterium, can tolerate the high temperature more than 75 ℃, and at high temperature brings into play the degradation capability of lignocellulose; The 2# composite fungus agent is had a liking for warm ligocellulose degradation bacterium and 5 strains by 10 strains and is had a liking for warm ligocellulose degradation fungi and form, and can quicken intermediate product mineralising and humification process in the middle gentle normal temperature stage performance ligocellulose degradation ability below 45 ℃.The compost treatment that is used for afforestation refuses such as agricultural solid wastes such as vegetables refuse, flower straw and dry branches and fallen leaves, beta pruning weeds, by accelerating the degraded and the conversion of lignocellulose, degradation process between collaborative different degradation capability refuses, reach the shortening fermentation time, promote compost maturity, improve quality of compost products.
Use composite fungus agent as the big requirement of inoculation microbial inoculum demand, the corresponding zymotechnique of researching and developing this composite fungus agent at pilot scale and batch production scale.The cultivation of composite fungus agent be with the sugaring soya-bean milk as the liquid state fermentation substratum or with materials such as sawdust rice brans as solid-state fermentation culture medium, by this technology, bulk fermentation production at short notice.Wherein the culture medium prescription of liquid state fermentation is new fresh soya-bean milk 10-50ml, and glucose 20g adds water to 1L, and pH6-8, the composite fungus agent inoculum size with 1-20% in this substratum can reach maximum harvest yield 10 in foster 40-70 hour 25 ℃-35 ℃ continuously sudden and violent down air cultures
9/ ml; And be sawdust 80%, rice bran 10%, wheat bran 10%, (NH at prescription
4)
2SO
41.5%, KH
2PO
40.5%, in the solid-state fermentation culture medium of water ratio 50%-60%, this composite fungus agent is cultivated with inoculum size turning at intermittence under 25 ℃-50 ℃ of 1-20% can reach maximum harvest yield 10 in 2 weeks
9/ g, production concentration all reach required standard.
The prescription of used liquid state fermentation substratum is that new fresh soya-bean milk is used 10ml, 20ml, 30ml, 40ml, 50ml respectively in concrete test, add glucose 20g, add water to 1L, pH can be adjusted to 6,7,8, composite fungus agent was supported 40,48,55,60,70 hours 25 ℃, 30 ℃, 35 ℃ continuously sudden and violent down air cultures with 1%, 5%, 10%, 15%, 20% inoculum size respectively in this substratum, all may reach maximum harvest yield 10
9/ ml; And be sawdust 80%, rice bran 10%, wheat bran 10%, (NH at prescription
4)
2SO
41.5%, KH
2PO
40.5%, in the solid-state fermentation culture medium of water ratio 50%-60%, this composite fungus agent is cultivated with inoculum size turning at intermittence under 25 ℃, 30 ℃, 35 ℃ of 1%, 5%, 10%, 15%, 20% respectively all may reach maximum harvest yield 10 in 2 weeks
9/ g, the physical form of its corresponding microbial inoculum product is: liquid microbial inoculum is the beige suspension liquid, pH is 8.0-8.5; Solid-state microbial inoculum is loose pale brown look sawdust form; Bacterial classification content in the product: liquid microbial inoculum bacteria containing amount in 12 months quality guaranteed perioves＞10
9/ ml, solid-state microbial inoculum＞10
9/ g.
During use, in the one time fermentation stage of compost, the inoculum size with 1% adds composite fungus agent 1# in compost material, and stirs.In order to make the microorganism in the 1# composite fungus agent keep active, bring into play best degradation function, the highest heap temperature of composting process should be controlled at below 75 ℃.When below the heap temperature drop to 40 ℃ or in the compost Secondary Fermentation stage, in compost material, add composite fungus agent 2# with 1% inoculum size again, continue composting, compost material is become thoroughly decomposed fully, become safety, health, physical behavior is good, nutrient content is high biological active organic fertilizer.
Through in Tsing-Hua University's Environmental Science and Engineering being repeatedly experiment and on-the-spot pilot scale in the laboratory in the big fish-farming area in Chenggong County, Kunming " research of Dianchi Lake Basin pollution of area source control techniques " demonstration area, the result shows, two groups of composite fungus agents are used and can improve warm 2-4 ℃ in heap than other biological and abiotic control treatment, the hot stage time length prolongs, strengthened the activity of overall micro-flora and ligocellulose degradation bacterium in the heap body, obviously quicken the associating compost process of vegetable or flower stalk, shorten and to become thoroughly decomposed the time, and (yield of grain diameter＜1cm) improves more than 30% to make final meticulous composting production.
Claims (2)
1. a lignocellulose solid waste composting is urged rotten composite fungus agent, it is characterized in that: described composite fungus agent comprises the microbial inoculum of composite fungus agent 1# and two models of 2#, be applicable to the hot stage of one time fermentation in the composting process and the normal temperature/temperature-fall period of Secondary Fermentation respectively, wherein the 1# composite fungus agent is made up of the thermophilic ligocellulose degradation of 4-8 strain bacterium, can tolerate 70 ℃-90 ℃ high temperature, and at high temperature bring into play the degradation capability of lignocellulose; The 2# composite fungus agent is had a liking for warm ligocellulose degradation bacterium and 4-8 strain by the 8-12 strain and is had a liking for warm ligocellulose degradation fungi and form, and can quicken intermediate product mineralising and humification process 50 ℃-15 ℃ middle gentle normal temperature stage performance ligocellulose degradation ability; The compost treatment that is used for afforestation refuses such as agricultural solid wastes such as vegetables refuse, flower straw and dry branches and fallen leaves, beta pruning weeds, by accelerating the degraded and the conversion of lignocellulose, degradation process between collaborative different degradation capability refuses, reach the shortening fermentation time, promote compost maturity, improve quality of compost products.
2. according to the short rotten composite fungus agent of the described lignocellulose solid waste of claim 1 composting, it is characterized in that: described composite fungus agent be with the sugaring soya-bean milk as the liquid state fermentation substratum or with materials such as sawdust rice brans as solid-state fermentation culture medium, fermentation culture is come out at short notice, wherein the culture medium prescription of liquid state fermentation is new fresh soya-bean milk 10-50ml, glucose 20g, add water to 1L, pH6-8, the composite fungus agent inoculum size with 1-20% in this substratum can reach maximum harvest yield 10 in foster 40-70 hour 25 ℃-35 ℃ continuously sudden and violent down air cultures
9/ ml; And be sawdust 80%, rice bran 10%, wheat bran 10%, (NH at prescription
4)
2SO
41.5%, KH
2PO
40.5%, in the solid-state fermentation culture medium of water ratio 50%-60%, this composite fungus agent is cultivated with inoculum size turning at intermittence under 25 ℃-50 ℃ of 1-20% can reach maximum harvest yield 10 in 2 weeks
9/ g; The physical form of its corresponding microbial inoculum product is: liquid microbial inoculum is the beige suspension liquid, and pH is 8.0-8.5; Solid-state microbial inoculum is loose pale brown look sawdust form; Bacterial classification content in the product: liquid microbial inoculum bacteria containing amount in 12 months quality guaranteed perioves＞10
9/ ml, solid-state microbial inoculum＞10
9/ g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03136613A CN1460664A (en) | 2003-05-19 | 2003-05-19 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
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|---|---|---|---|
| CN03136613A CN1460664A (en) | 2003-05-19 | 2003-05-19 | Livnocellulose solid wastes composting decomposition-promoting composite bacterial preparation |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101054552B (en) * | 2007-04-05 | 2010-06-09 | 清华大学 | Microorganism composite bacterium agent and preparation method and application thereof |
| CN101735962B (en) * | 2008-11-12 | 2012-05-23 | 中国农业大学 | Low temperature fermentation inoculum for preparing straw feed |
| CN102492621A (en) * | 2011-11-16 | 2012-06-13 | 天津工业生物技术研究所 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
| CN101759467B (en) * | 2008-12-24 | 2012-10-17 | 中国环境科学研究院 | Rural solid waste mixed aerobic composting method |
| CN1934249B (en) * | 2004-01-16 | 2012-11-14 | 诺维信股份有限公司 | Methods for degrading lignocellulosic materials |
| CN106701603A (en) * | 2017-03-18 | 2017-05-24 | 云南博隆生物科技开发有限公司 | Preparation method of high-efficient decay-promoting agent |
| CN106938949A (en) * | 2017-04-10 | 2017-07-11 | 山东米高生物科技股份有限公司 | A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer |
| CN110468119A (en) * | 2019-07-01 | 2019-11-19 | 福建美天环保科技有限公司 | A kind of garbage disposal microbial activity medium carrier |
| CN111528043A (en) * | 2020-05-09 | 2020-08-14 | 武汉华天园林艺术有限公司 | Method for cultivating green nursery stock by using garden waste |
| CN112481130A (en) * | 2020-11-28 | 2021-03-12 | 南京大学（溧水）生态环境研究院 | Method for extracting and preparing efficient composite composting microbial inoculum from cow dung |
-
2003
- 2003-05-19 CN CN03136613A patent/CN1460664A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1934249B (en) * | 2004-01-16 | 2012-11-14 | 诺维信股份有限公司 | Methods for degrading lignocellulosic materials |
| CN101054552B (en) * | 2007-04-05 | 2010-06-09 | 清华大学 | Microorganism composite bacterium agent and preparation method and application thereof |
| CN101735962B (en) * | 2008-11-12 | 2012-05-23 | 中国农业大学 | Low temperature fermentation inoculum for preparing straw feed |
| CN101759467B (en) * | 2008-12-24 | 2012-10-17 | 中国环境科学研究院 | Rural solid waste mixed aerobic composting method |
| CN102492621A (en) * | 2011-11-16 | 2012-06-13 | 天津工业生物技术研究所 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
| CN102492621B (en) * | 2011-11-16 | 2013-01-23 | 天津工业生物技术研究所 | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer |
| CN106701603A (en) * | 2017-03-18 | 2017-05-24 | 云南博隆生物科技开发有限公司 | Preparation method of high-efficient decay-promoting agent |
| CN106938949A (en) * | 2017-04-10 | 2017-07-11 | 山东米高生物科技股份有限公司 | A kind of preparation method for loading high concentration bacillus subtilis kitchen garbage organic fertilizer |
| CN110468119A (en) * | 2019-07-01 | 2019-11-19 | 福建美天环保科技有限公司 | A kind of garbage disposal microbial activity medium carrier |
| CN111528043A (en) * | 2020-05-09 | 2020-08-14 | 武汉华天园林艺术有限公司 | Method for cultivating green nursery stock by using garden waste |
| CN112481130A (en) * | 2020-11-28 | 2021-03-12 | 南京大学（溧水）生态环境研究院 | Method for extracting and preparing efficient composite composting microbial inoculum from cow dung |
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