CN109182223A - A kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly - Google Patents
A kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly Download PDFInfo
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- CN109182223A CN109182223A CN201811263342.7A CN201811263342A CN109182223A CN 109182223 A CN109182223 A CN 109182223A CN 201811263342 A CN201811263342 A CN 201811263342A CN 109182223 A CN109182223 A CN 109182223A
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- bacterium
- microbial inoculum
- complex microbial
- bacillus
- environment friendly
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention discloses a kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly, including photosynthetic bacteria, kluyveromyces marxianus bacterium, bacillus subtilis, bacillus licheniformis, Lactobacillus delbrueckii, Bacillus alcaligenes, thermoactinomyces vulgaris, bacterium for producing flocculant, ammonia nitrogen degradation bacterium, phosphate solubilizing bacteria, Thiobacillus thioxidans shows pyrenomycetes and auxiliary element.Stink can be greatly removed, the decomposed of rubbish is accelerated and is stablized, inhibits the generation and release of new stench, treatment effeciency height, long action time are without secondary pollution, easy to operate, low-cost.
Description
Technical field
The present invention relates to a kind of complex microbial inoculum, in particular to a kind of method with biology carries out rubbish deodorization
Composite bacteria agent belongs to environment protection field.
Background technique
With the rapid development of social economy and the continuous acceleration of urbanization process, environmental pollution caused by rubbish stench is asked
Topic becomes increasingly conspicuous, and directly affects people's lives quality, or even jeopardize people's health.Odor treatment research is with Japan, lotus
The states such as orchid, Germany are the most advanced.Currently, the minimizing technology for stink mainly has: (1) (oxidizing process absorbs chemical deodorization method
Method, absorption method) it include combustion method (flame combustion, catalysis burning)；(2) physical deodorization method such as buries method, dilution diffusion method etc.；
(3) biological odor removal method mainly utilizes microbial deodorant, will have substance frowzy by the physiological metabolism of microorganism and turned
Change.And the technology of biological deodorizing also gradually vigorously has developed in field of environmental improvement by feat of its incomparable superiority
Come.It can be used for eliminating swill treatment, garbage disposal, animal wastes processing, compound fertilizer composting, livestock and poultry cultivation production, septic tank, lavatory
Institute, greasy dirt pond, sewer, dustbin, garbage truck, restaurant, hotel, room, hospital, city have stable political situation agricultural etc. generate ammonia,
The control and deodorization of other toxic and harmful gas such as hydrogen sulfide.It is toxic that ammonia, hydrogen sulfide etc. are generated using microorganism and mould degradation
Pernicious gas controls the generation of pernicious gas from source and eliminates gas pernicious gas.It is simple and safe to use, to people, to animal
With it is environmentally friendly.
Rubbish stench is controlled there are two types of approach: (1) in the rubbish not degenerated be added beneficial bacterium and cellulose-decomposing bacterium,
Lignin fungi etc. forms high-effective microorganism Inoculant, carries out compost, is allowed to be converted to fertilizer re-using.(2) rotten
Change and microbial flora is added in the rubbish of foul smelling, controls stench.
Although deodorant currently on the market has certain effect, but only with single or only several frequently seen micro- life
Object bacterium, some even can form secondary pollution with the microbial interaction generated in rubbish.Some deodorant cannot effectively be gone
Except the foul smell of difference wastes all kinds of in rubbish, the production for the malodorant that can not effectively inhibit rubbish new in composting treatment process
It is raw, to accelerate compost at production process, shorten the production cycle.Therefore, the deodorizing microorganism for how providing a kind of high-efficiency environment friendly is multiple
Combined bacteria agent is problem to be solved.
Summary of the invention
Based on the problems of prior art, the object of the present invention is to provide a kind of rubbish deodorizations of high-efficiency environment friendly with micro-
Biological compound fungi agent.
In order to achieve the object of the present invention and advantage, the invention is realized by the following technical scheme:
Embodiment of the present invention provides a kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly, including photosynthetic bacteria,
Kluyveromyces marxianus bacterium, bacillus subtilis, bacillus licheniformis, Lactobacillus delbrueckii, Bacillus alcaligenes, common high temperature unwrapping wire
Bacterium, bacterium for producing flocculant, phosphate solubilizing bacteria, Thiobacillus thioxidans show pyrenomycetes and auxiliary element.
Auxiliary element in the complex microbial inoculum includes tartaric acid, oxalic acid, salicylic acid, active carbon, ceramic grain etc..
In the complex microbial inoculum, photosynthetic bacteria, kluyveromyces marxianus bacterium, bacillus subtilis, lichens gemma
Bacillus, Lactobacillus delbrueckii, Bacillus alcaligenes, thermoactinomyces vulgaris, bacterium for producing flocculant, phosphate solubilizing bacteria, Thiobacillus thioxidans show core
The proportion of bacterium and auxiliary element are as follows: 1~3:1~3:0.5~1.5:0.5~1.5:0.5~1.5:1~3:2~3:0.5~1:1
~3:1~3:1~3:4~8.
The proportion of auxiliary element in the complex microbial inoculum is: 1~3:1~3:1~3:5~10:5~10.
The production method of the complex microbial inoculum is the following steps are included: 1. by photosynthetic bacteria, kluyveromyces marxianus
Bacterium, bacillus subtilis, bacillus licheniformis, Lactobacillus delbrueckii, Bacillus alcaligenes, thermoactinomyces vulgaris, bacterium for producing flocculant,
Phosphate solubilizing bacteria, Thiobacillus thioxidans, the strain for showing pyrenomycetes is obtained by routine techniques respectively, then carries out slant activation, then respectively
It is inoculated into fermented and cultured in suitable culture medium, the concentration for finally obtaining every kind of bacterium is no less than 107The bacterium solution of CFU/mL；2. by each
The bacterium solution and auxiliary element of kind bacterium mix mixing according to the proportion and the complex microbial inoculum are made.
Optionally, pulvis can be made in affiliated complex microbial inoculum.
The present invention can produce following the utility model has the advantages that stink can be removed greatly, make dirt, organic substance rapid new old generation
It thanks, accelerate the decomposed of rubbish and stablizes, inhibit the generation and release of new stench, treatment effeciency height, long action time, without secondary
It pollutes, is easy to operate, low-cost, improving the environment of composting plant.
Specific embodiment
The present invention is done below with reference to embodiment and is further explained, to enable those skilled in the art referring to specification text
Word can be according to implement.The following example is merely to illustrate the present invention, but is not used to limit practical range of the invention.
Embodiment 1:
By photosynthetic bacteria, kluyveromyces marxianus bacterium, bacillus subtilis, bacillus licheniformis, Lactobacillus delbrueckii, production alkali
The bacterial strain of bacillus, thermoactinomyces vulgaris, bacterium for producing flocculant, phosphate solubilizing bacteria, Thiobacillus thioxidans, aobvious pyrenomycetes is respectively connected to accordingly
Fluid nutrient medium in, at 25 DEG C, on the shaking table of 150r/min carry out single plant expand shaking flask culture, until bacterial concentration reaches
107CFU/mL。
Every kind of bacterium solution is mixed according to the ratio of 1:1:0.5:0.5:0.5:1:2:1:1:1:1, then carries out shaking table training
It supports for 24 hours, forms composite bacteria liquid.
By tartaric acid, oxalic acid, salicylic acid, active carbon, ceramic grain mixed according to the ratio of 1:1:1:5:5, then plus
Enter composite bacteria liquid mixing, can be obtained heretofore described complex microbial inoculum.
Embodiment 2:
By photosynthetic bacteria, kluyveromyces marxianus bacterium, bacillus subtilis, bacillus licheniformis, Lactobacillus delbrueckii, production alkali
The bacterial strain of bacillus, thermoactinomyces vulgaris, bacterium for producing flocculant, phosphate solubilizing bacteria, Thiobacillus thioxidans, aobvious pyrenomycetes is respectively connected to accordingly
Fluid nutrient medium in, at 35 DEG C, on the shaking table of 180r/min carry out single plant expand shaking flask culture, until bacterial concentration reaches
108CFU/mL。
Every kind of bacterium solution is mixed according to the ratio of 1:1:1:1:1:1:2:0.5:1:1:1, then carries out shaking table culture
48h forms composite bacteria liquid.
By tartaric acid, oxalic acid, salicylic acid, active carbon, ceramic grain mixed according to the ratio of 1.5:1.5:1.5:10:10
It closes, composite bacteria liquid mixing is then added, can be obtained heretofore described complex microbial inoculum.
Comparative example:
By photosynthetic bacteria, Bacillus alcaligenes, ammonia nitrogen degradation bacterium, the bacterial strain of phosphate solubilizing bacteria is respectively connected in corresponding fluid nutrient medium,
Single plant is carried out at 35 DEG C, on the shaking table of 180r/min and expands shaking flask culture, until bacterial concentration is up to 108CFU/mL。
Every kind of bacterium solution is mixed according to the ratio of 1:1:1:1, then carries out shaking table culture 48h, forms composite bacteria liquid.
By oxalic acid, active carbon is mixed according to the ratio of 1:10, and above-mentioned composite bacteria liquid mixing is then added, is compared
Complex microbial inoculum.
Application Example:
By in the present invention Examples 1 and 2 and comparative example be applied to certain cell Biohazard Waste Disposal, select the obvious concentration of peculiar smell
And close rubbish heap everywhere, each planning processing area 5m2Rubbish, two o'clock is respectively by Examples 1 and 2 and comparative example in the afternoon
In complex microbial inoculum correspondence be uniformly sprayed at each rubbish heap surface, in addition hurrock what do not spray, as sky
White control.3 days, 5 days, 7 days each NH checked in rubbish heap after spraying3And H2S concentration.Experimental result is as shown in table 1.
Table 1
From experimental result as can be seen that the microbial composite bacteria of the sprinkling embodiment of the present invention 1 and 2, one week interior to rubbish heap
In NH3And H2S concentration can make its lasting reduction about 80%, and removal effect is obviously more micro- than being made into comparative example by several bacterium
Biological compound fungi agent will be got well.Show that the generation and release of stench in rubbish can be effectively suppressed in complex microbial inoculum of the invention,
Treatment effeciency height, long action time, it is without secondary pollution, easy to operate, hence it is evident that improve the environment of composting plant.
Claims (5)
1. a kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly characterized by comprising photosynthetic bacteria, Marx gram
Tie up saccharomycete, bacillus subtilis, bacillus licheniformis, Lactobacillus delbrueckii, Bacillus alcaligenes, thermoactinomyces vulgaris, flocculation in Shandong
Agent producing strains, phosphate solubilizing bacteria, Thiobacillus thioxidans show pyrenomycetes and auxiliary element.
2. a kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly according to claim 1, it is characterised in that: institute
State photosynthetic bacteria in complex microbial inoculum, kluyveromyces marxianus bacterium, bacillus subtilis, bacillus licheniformis, De Shi cream
Bacillus, Bacillus alcaligenes, thermoactinomyces vulgaris, bacterium for producing flocculant, phosphate solubilizing bacteria, Thiobacillus thioxidans, show pyrenomycetes and auxiliary at
The proportion divided are as follows: 1~3:1~3:0.5~1.5:0.5~1.5:0.5~1.5:1~3:2~3:0.5~1:1~3:1~3:1
~3:4~8.
3. a kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly according to claim 1, it is characterised in that: institute
The auxiliary element for stating complex microbial inoculum includes tartaric acid, oxalic acid, salicylic acid, active carbon, ceramic grain.
4. a kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly according to claim 1, it is characterised in that: institute
The proportion for stating the auxiliary element in complex microbial inoculum is: 1~3:1~3:1~3:5~10:5~10.
5. a kind of rubbish deodorization complex microbial inoculum using a kind of high-efficiency environment friendly described in claim 1, feature
It is, is realized by following steps:
1. by photosynthetic bacteria, kluyveromyces marxianus bacterium, bacillus subtilis, bacillus licheniformis, Lactobacillus delbrueckii, production alkali bar
The strain of bacterium, thermoactinomyces vulgaris, bacterium for producing flocculant, phosphate solubilizing bacteria, Thiobacillus thioxidans, aobvious pyrenomycetes passes through conventional skill respectively
Art obtains, then carries out slant activation, is then respectively inoculated into fermented and cultured in suitable culture medium, finally obtains the dense of every kind of bacterium
Degree no less than 107The bacterium solution of CFU/mL；
2. the bacterium solution of various bacterium and auxiliary element are mixed to mixing according to the proportion is made the complex microbial inoculum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811263342.7A CN109182223A (en) | 2018-10-28 | 2018-10-28 | A kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811263342.7A CN109182223A (en) | 2018-10-28 | 2018-10-28 | A kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly |
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| Publication Number | Publication Date |
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| CN109182223A true CN109182223A (en) | 2019-01-11 |
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| CN201811263342.7A Withdrawn CN109182223A (en) | 2018-10-28 | 2018-10-28 | A kind of rubbish deodorization complex microbial inoculum of high-efficiency environment friendly |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112501060A (en) * | 2020-11-23 | 2021-03-16 | 扬州鑫威环保生物科技有限公司 | Microbial agent for efficiently degrading kitchen waste at high temperature and application method thereof |
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| CN102430142A (en) * | 2011-12-22 | 2012-05-02 | 上海环境工程技术有限公司 | Composite ecological deodorant for stink gas control |
| CN102578156A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof |
| CN104258440A (en) * | 2014-09-04 | 2015-01-07 | 随州新欣农业有限公司 | Biological deodorant and preparation method thereof |
| CN107164284A (en) * | 2017-07-10 | 2017-09-15 | 佛山市水创科联生态科技有限公司 | A kind of deodorizing microorganism microbial inoculum and preparation method thereof |
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2018
- 2018-10-28 CN CN201811263342.7A patent/CN109182223A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102430142A (en) * | 2011-12-22 | 2012-05-02 | 上海环境工程技术有限公司 | Composite ecological deodorant for stink gas control |
| CN102578156A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof |
| CN104258440A (en) * | 2014-09-04 | 2015-01-07 | 随州新欣农业有限公司 | Biological deodorant and preparation method thereof |
| CN107164284A (en) * | 2017-07-10 | 2017-09-15 | 佛山市水创科联生态科技有限公司 | A kind of deodorizing microorganism microbial inoculum and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112501060A (en) * | 2020-11-23 | 2021-03-16 | 扬州鑫威环保生物科技有限公司 | Microbial agent for efficiently degrading kitchen waste at high temperature and application method thereof |
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