CN112501060A - Microbial agent for efficiently degrading kitchen waste at high temperature and application method thereof - Google Patents
Microbial agent for efficiently degrading kitchen waste at high temperature and application method thereof Download PDFInfo
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- CN112501060A CN112501060A CN202011318609.5A CN202011318609A CN112501060A CN 112501060 A CN112501060 A CN 112501060A CN 202011318609 A CN202011318609 A CN 202011318609A CN 112501060 A CN112501060 A CN 112501060A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 33
- 239000010806 kitchen waste Substances 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000000593 degrading effect Effects 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 claims abstract description 24
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 22
- 239000007857 degradation product Substances 0.000 claims abstract description 22
- 238000006731 degradation reaction Methods 0.000 claims abstract description 22
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 19
- 230000015556 catabolic process Effects 0.000 claims abstract description 18
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 15
- 241000203775 Thermoactinomyces Species 0.000 claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 7
- 235000013339 cereals Nutrition 0.000 claims description 5
- 235000013372 meat Nutrition 0.000 claims description 5
- 235000013311 vegetables Nutrition 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 4
- 241000186046 Actinomyces Species 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 13
- 238000005516 engineering process Methods 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 6
- 239000010813 municipal solid waste Substances 0.000 abstract description 6
- 238000000354 decomposition reaction Methods 0.000 abstract description 3
- 239000000047 product Substances 0.000 abstract description 3
- 241000186361 Actinobacteria <class> Species 0.000 abstract 1
- 239000000376 reactant Substances 0.000 description 24
- 239000010985 leather Substances 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 6
- 239000012876 carrier material Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000004817 gas chromatography Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical group O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000361919 Metaphire sieboldi Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005416 organic matter Chemical group 0.000 description 1
- 235000015927 pasta Nutrition 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention is applicable to the technical field of microbial degradation, and provides a microbial agent for efficiently degrading kitchen waste at high temperature and an application method thereof, wherein the microbial agent comprises bacillus subtilis, bacillus licheniformis and high-temperature actinomycetes, and the preparation conditions comprise the following steps: the culture temperature is as follows: 50 ℃ to 80 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10, the invention solves the problems that the strain adopted by the existing microbial treatment technology has low high temperature resistance, liquid can be generated in the decomposition product, simultaneously the residual bacteria in the garbage are bred, and the discharge of the degradation product can cause secondary pollution to the environment.
Description
Technical Field
The invention belongs to the field of microbial degradation, and particularly relates to a microbial agent for efficiently degrading kitchen waste at a high temperature and an application method thereof.
Background
With the rapid development of social economy, the level of living matters is continuously improved, how to efficiently and rapidly treat daily garbage becomes a hot topic, the daily output of kitchen garbage in China exceeds 2 ten thousand tons, wherein the output of household kitchen garbage reaches 1.2 ten thousand tons, at present, the domestic and foreign garbage treatment method mainly takes traditional incineration and landfill as main materials, and newly-developed compost, earthworm treatment, methane fermentation and the like are tested in a small range.
The microbial treatment technology is still in a development stage, strains adopted by the existing microbial treatment technology have low high temperature resistance and low strength, liquid can be generated in decomposition products, meanwhile, residual bacteria in garbage are bred, and secondary pollution to the environment can be caused by the discharge of degradation products.
Disclosure of Invention
The invention provides a microbial agent for high-temperature and high-efficiency degradation of kitchen waste and an application method thereof, and aims to solve the problems that a microbial treatment technology is in a development stage, strains adopted by the existing microbial treatment technology have low high-temperature resistance and low strength, liquid can be generated in decomposition products, meanwhile, residual bacteria in the waste are bred, and secondary pollution to the environment can be caused by the discharge of the degradation products.
The invention is realized in such a way that a microbial agent for efficiently degrading kitchen waste at high temperature comprises bacillus subtilis, bacillus licheniformis and high-temperature actinomyces, and comprises the following preparation conditions: the culture temperature is as follows: 50 ℃ to 80 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
preferably, the following preparation conditions are included: the culture temperature is as follows: 50 ℃ to 60 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
preferably, the following preparation conditions are included: the culture temperature is as follows: 60 ℃ to 70 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
preferably, the following preparation conditions are included: the culture temperature is as follows: 70-80 ℃, bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
an application method of a microbial agent for efficiently degrading kitchen waste at high temperature comprises the following steps:
step S100: preparing a microbial inoculum;
step S200: preparing degradation products;
step S300: setting the temperature;
step S400: measuring the pH value;
step S500: detecting gas;
step S600: and (5) detecting degradation reaction.
Preferably, in the step S100, the preparation carrier of the microbial inoculum is saw powder and activated carbon, and the mass ratio of the saw powder to the activated carbon is 10: 1.
preferably, in step S200, the main components of the degradation product are cereals, pasta, meat and vegetables.
Preferably, in step S300, the temperature setting range is: 50 ℃ to 80 ℃.
Preferably, in step S500, the gas is collected by a gas collecting bag.
Preferably, in step S600, the degradation reaction is specifically detected by detecting a reduction rate and a degradation degree.
Compared with the prior art, the invention has the beneficial effects that: according to the microbial agent for degrading the kitchen waste at high temperature and high efficiency and the application method thereof, provided by the invention, on the premise of the original microbial treatment technology, the microbial agent which is high in temperature resistance and high in efficiency can be provided, so that the kitchen waste can be directly degraded into carbon dioxide and organic matter residues without bacteria under the high-temperature condition, the secondary pollution of the residues is avoided, and the reduction rate of waste degradation is improved.
Drawings
FIG. 1 is a flow chart of an application method of the present invention;
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1:
the microbial agent for efficiently degrading kitchen waste at high temperature comprises the following preparation conditions: the culture temperature is as follows: 50 ℃ to 60 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
in the embodiment, the three strains are inoculated and expanded for culture, the concentration of the bacteria liquid is measured, the cultured bacterial suspension is centrifuged for 10min at 6000r/min, 2/3 supernatant is discarded, the rest part is uniformly shaken for standby, and the mass ratio of the sawdust powder to the active carbon is 10: 1 as a carrier material, and mixing the prepared concentrated bacterial suspension according to the mass ratio of 1: 1: 1, uniformly spraying the mixture on a carrier material to prepare a bacterium preparation, wherein the main components of degradation products comprise cereals, wheaten food, meat and vegetables, and the bacterium preparation is prepared by mixing the following components in a ratio of 1: 10, then dividing the mixture into ten groups, respectively placing the groups into a culture environment at 50-60 ℃, after reacting for a period of time, taking the reactant to measure the pH value of the reactant, weighing 5g of the reactant, placing the reactant into a 50mL small beaker, adding 25mL of distilled water, intermittently stirring for 30min, placing the beaker for 30min, measuring the reactant by using an acidimeter, taking the average number of 3 measurements, collecting gas at a common exhaust port every 2h by using a gas collection bag (or an injector sleeved with a leather hose) during the period, collecting the tail gas of a processor by using a 60mL injector sleeved with a leather hose, clamping the leather hose to seal the injector, detecting and analyzing the components in the tail gas by using gas chromatography, observing the degradation degree of the reactant when the degradation is ended, measuring and calculating the degradation degree of the reactant, recording the degradation time of the reactant, and selecting the optimal microbial inoculum to be put into application, thereby preparing the high-temperature efficient microbial.
Example 2:
the microbial agent for efficiently degrading kitchen waste at high temperature comprises the following preparation conditions: the culture temperature is as follows: 60 ℃ to 70 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
in the embodiment, the three strains are inoculated and expanded for culture, the concentration of the bacteria liquid is measured, the cultured bacterial suspension is centrifuged for 10min at 6000r/min, 2/3 supernatant is discarded, the rest part is uniformly shaken for standby, and the mass ratio of the sawdust powder to the active carbon is 10: 1 as a carrier material, and mixing the prepared concentrated bacterial suspension according to the mass ratio of 1: 1: 1, uniformly spraying the mixture on a carrier material to prepare a bacterium preparation, wherein the main components of degradation products comprise cereals, wheaten food, meat and vegetables, and the bacterium preparation is prepared by mixing the following components in a ratio of 1: 10, then dividing the mixture into ten groups, respectively placing the groups into a culture environment at 60-70 ℃, after reacting for a period of time, taking the reactant to measure the pH value of the reactant, weighing 5g of the reactant, placing the reactant into a 50mL small beaker, adding 25mL of distilled water, intermittently stirring for 30min, placing the beaker for 30min, measuring the reactant by using an acidimeter, taking the average of 3 measurements, collecting gas at a common exhaust port every 2h by using a gas collection bag (or an injector sleeved with a leather hose) during the period, collecting the tail gas of a processor by using a 60mL injector sleeved with a leather hose, clamping the leather hose to seal the injector, detecting and analyzing the components in the tail gas by using gas chromatography, observing the degradation degree of the reactant when the degradation is ended, measuring and calculating the degradation degree of the reactant, recording the degradation time of the reactant, and selecting the optimal microbial inoculum to be put into application, thereby preparing the high-temperature and high.
Example 3:
the microbial agent for efficiently degrading kitchen waste at high temperature comprises the following preparation conditions: the culture temperature is as follows: 70-80 ℃, bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
in the embodiment, the three strains are inoculated and expanded for culture, the concentration of the bacteria liquid is measured, the cultured bacterial suspension is centrifuged for 10min at 6000r/min, 2/3 supernatant is discarded, the rest part is uniformly shaken for standby, and the mass ratio of the sawdust powder to the active carbon is 10: 1 as a carrier material, and mixing the prepared concentrated bacterial suspension according to the mass ratio of 1: 1: 1, uniformly spraying the mixture on a carrier material to prepare a bacterium preparation, wherein the main components of degradation products comprise cereals, wheaten food, meat and vegetables, and the bacterium preparation is prepared by mixing the following components in a ratio of 1: 10, then dividing the mixture into ten groups, respectively placing the groups into a culture environment at 70-80 ℃, after reacting for a period of time, taking the reactant to measure the pH value of the reactant, weighing 5g of the reactant, placing the reactant into a 50mL small beaker, adding 25mL of distilled water, intermittently stirring for 30min, placing the beaker for 30min, measuring the reactant by using an acidimeter, taking the average number of 3 measurements, collecting gas at a common exhaust port every 2h by using a gas collection bag (or an injector sleeved with a leather hose) during the period, collecting the tail gas of a processor by using a 60mL injector sleeved with a leather hose, clamping the leather hose to seal the injector, detecting and analyzing the components in the tail gas by using gas chromatography, observing the degradation degree of the reactant when the degradation is ended, measuring and calculating the degradation degree of the reactant, recording the degradation time of the reactant, and selecting the optimal microbial inoculum to be put into application, thereby preparing the high-temperature efficient microbial.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. The microbial agent for efficiently degrading kitchen waste at high temperature comprises bacillus subtilis, bacillus licheniformis and high-temperature actinomyces, and is characterized in that: the preparation method comprises the following preparation conditions: the culture temperature is as follows: 50 ℃ to 80 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
2. the microbial agent for efficiently degrading kitchen waste at high temperature according to claim 1, wherein: the preparation method comprises the following preparation conditions: the culture temperature is as follows: 50 ℃ to 60 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
3. the microbial agent for efficiently degrading kitchen waste at high temperature according to claim 1, wherein: the preparation method comprises the following preparation conditions: the culture temperature is as follows: 60 ℃ to 70 ℃, and bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
4. the microbial agent for efficiently degrading kitchen waste at high temperature according to claim 1, wherein: the preparation method comprises the following preparation conditions: the culture temperature is as follows: 70-80 ℃, bacillus subtilis: 6.0 x 10^6ug/g, Bacillus licheniformis: 6.0 x 10^6ug/g, Thermoactinomyces: 6.0 x 10^6ug/g, the rest is culture medium, the mass ratio of the microbial inoculum to the degradation product is as follows: 1: 10.
5. an application method of a microbial agent for efficiently degrading kitchen waste at high temperature is characterized by comprising the following steps: the method comprises the following steps:
step S100: preparing a microbial inoculum;
step S200: preparing degradation products;
step S300: setting the temperature;
step S400: measuring the pH value;
step S500: detecting gas;
step S600: and (5) detecting degradation reaction.
6. The application method of the microbial agent for efficiently degrading the kitchen waste at high temperature according to claim 5, wherein the application method comprises the following steps: in the step S100, the preparation carrier of the microbial inoculum is saw powder and activated carbon, and the mass ratio of the saw powder to the activated carbon is 10: 1.
7. the application method of the microbial agent for efficiently degrading the kitchen waste at high temperature according to claim 5, wherein the application method comprises the following steps: in step S200, the main components of the degradation product are cereals, wheaten food, meat and vegetables.
8. The application method of the microbial agent for efficiently degrading the kitchen waste at high temperature according to claim 5, wherein the application method comprises the following steps: in step S300, the temperature setting range is: 50 ℃ to 80 ℃.
9. The application method of the microbial agent for efficiently degrading the kitchen waste at high temperature according to claim 5, wherein the application method comprises the following steps: in step S500, the gas is collected by a gas collecting bag.
10. The application method of the microbial agent for efficiently degrading the kitchen waste at high temperature according to claim 5, wherein the application method comprises the following steps: in step S600, the degradation reaction is specifically detected as a reduction rate and a degradation degree.
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Effective date of registration: 20211126 Address after: No.50, xinganquan East Road, Yangzhou City, Jiangsu Province, 225000 Applicant after: YANGZHOU JINWEI ENVIRONMENTAL PROTECTION TECHNOLOGY Co.,Ltd. Address before: 225000 Building 1, No.50, East xinganquan Road, Hanjiang District, Suzhou City, Jiangsu Province Applicant before: Yangzhou Xinwei environmental protection Biotechnology Co.,Ltd. |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210316 |