CN104840987A - Composite microbial deodorant and preparation method thereof - Google Patents
Composite microbial deodorant and preparation method thereof Download PDFInfo
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- CN104840987A CN104840987A CN201410658144.6A CN201410658144A CN104840987A CN 104840987 A CN104840987 A CN 104840987A CN 201410658144 A CN201410658144 A CN 201410658144A CN 104840987 A CN104840987 A CN 104840987A
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Abstract
The present invention discloses a composite microbial deodorant and a preparation method thereof. The composite microbial deodorant contains Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Streptomyces griseus and Bacillus subtilis, wherein the Rhodop seudanonas palustris, the Candida utilis, and the Lactobacillus acidophilus are subjected to mixed fermentation in a light-transmitting temperature-controllable fermentation tank, the Streptomyces griseus and the Bacillus subtilis are subjected to mixed fermentation in an aerating sterile air temperature-controlled fermentation tank, and the two fermented bacterial agents are mixed and added with essence, citric acid and glacial acetic acid to prepare the composite microbial deodorant. The composite microbial deodorant of the present invention has characteristics of simple preparation process and no secondary pollution, and provides good odor removing effects for toilets, farms and other occasions.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of compound microbial deodorizer and preparation method thereof.
Background technology
Current most of communal bathroom is large due to flow of the people, cleans not in time, causes abnormal flavour between lavatory large, especially with the urine smell taste that urine causes.Equally, in a lot of plant, the feces that poultry is discharged, owing to clearing up in time, inevitably produces stench, attracts fly class, have a strong impact on raiser's physical and mental health.
This type of stink is primarily of compositions such as ammonia, volatility amine, partial vulcanization things (as hydrogen sulfide, sulfur dioxide).
Summary of the invention
For solving the problem, the invention provides a kind of deodorizing effect obvious, preparation cost is low, and not easily to environment cause secondary pollution for compound microbial deodorizer eliminating toilet and plant's stench and preparation method thereof.
Compound microbial deodorizer of the present invention, wherein include Rhodopseudomonas palustris (
rhodop seudanonas palustris), Candida utilis (
candida utilis), bacillus acidophilus (
lactobacillus acidophilus), streptomyces griseus (
streptomyces griseus), bacillus subtilis (
bacillus subtilis).
Strain of the present invention all belongs to and finds strain, all can buy from Chinese General Microbiological Culture preservation administrative center (CGMCC).
Between microorganism contained by compound microbial deodorizer of the present invention, mutual not antagonism, has stronger synergism.Wherein, described Rhodopseudomonas palustris (
rhodop seudanonas palustris) belong to the one of photosynthetic bacteria, there is strong adaptability, can effectively absorb containing nitrogen compound, to various organic matter, there is stronger decomposition and conversion capability, simultaneously also for other microorganisms provide nutrient substance.Described Candida utilis (
candida utilis) using carbamide etc. as nitrogenous source, ammonia release can be reduced.Described bacillus acidophilus (
lactobacillus acidophilus) can antibiotic agents be secreted, effectively suppress the generation of pathogenic bacterium.Described streptomyces griseus (
streptomyces griseus) antibiotic can be secreted at moderate temperatures, suppress the growth of putrefactive microorganisms.Described bacillus subtilis (
bacillus subtilis) Organic substance such as available protein, multiple sugar, reduce abnormal flavour and produce.
The invention still further relates to the preparation method of compound microbial deodorizer of the present invention, concrete steps are:
The first step: Rhodopseudomonas palustris, Candida utilis, bacillus acidophilus, streptomyces griseus, bacillus subtilis are carried out activation culture respectively.
Second step: will be that sterilized water forms mixed bacteria liquid I and is inoculated in the culture medium after sterilizing 1 according to the ratio being not less than mass ratio 1:50 by Rhodopseudomonas palustris 20 ~ 30wt%, Candida utilis 20 ~ 30wt%, bacillus acidophilus 20 ~ 30wt% and surplus, cultivates under certain condition and within 2 ~ 3 days, obtains composite bacteria agent capable basis stock solution A in fermentation tank.
Described culture medium 1 is the molasses culture medium improved, and soaks juice 0.5wt% by molasses 1wt%, bean sprout, the sterilized water composition of yeast extract 0.5wt%, sodium citrate 0.2wt%, potassium dihydrogen phosphate 0.1wt% and surplus, and pH value is 6 ~ 7; Described fermentation tank is glass or the acrylic sealing and fermenting tank of transparent controllable temperature; Described certain condition is that fermentation tank is in intensity of illumination 2000 ~ 3000lx, temperature is in the environment of 30 ~ 35 DEG C.In described composite bacteria agent capable basis stock solution A, Rhodopseudomonas palustris should not be less than 1 × 10
8individual/ml, Candida utilis should not be less than 1 × 10
8individual/ml, bacillus acidophilus should not be less than 2 × 10
8individual/ml.
3rd step: the sterilized water by streptomyces griseus 10 ~ 15wt%, bacillus subtilis 20 ~ 30wt% and surplus is formed mixed bacteria liquid II and is inoculated in the culture medium after sterilizing 2 according to the ratio being not less than mass ratio 1:50, cultivates under certain condition and within 1 ~ 2 day, obtains composite bacteria agent capable basis stock solution B in fermentation tank.
Described culture medium 2 is made up of soluble starch 1wt%, peptone 0.5wt%, Carnis Bovis seu Bubali cream 0.5wt%, sodium chloride 0.5wt%, trace element solution 1wt%; Described trace element is made up of the sterilized water of magnesium sulfate 0.5wt%, ferrous sulfate 0.1wt%, dipotassium hydrogen phosphate 5% and surplus, and pH value is 6 ~ 8; Described fermentation tank is for exposing to the sun filtrated air formula temperature controlled fermentation tank; Described certain condition is that the filtrated air aeration rate of fermentation tank is 10 ~ 20L/min, temperature is 30 ~ 35 DEG C.In described composite bacteria agent capable basis stock solution B, streptomyces griseus should not be less than 1 × 10
7individual/ml, bacillus subtilis should not be less than 2 × 10
8individual/ml.
4th step: by obtain in above-mentioned steps composite bacteria agent capable basis stock solution A 40 ~ 45wt%, composite bacteria agent capable basis stock solution B 40 ~ 45wt%, essence 0.05wt%, citric acid 0.5wt%, glacial acetic acid 0.5 wt% and surplus sterilized water mixing, finally obtain compound microbial deodorizer of the present invention.Described essence is conventional essence in this area.
The invention has the beneficial effects as follows: accelerate easily corrupt organic degraded, suppress the breeding of putrefactive microorganisms, change the metabolic pathway of the element such as nitrogen, sulfur, decrease the release of ammonia, hydrogen sulfide and volatile malodorous organic gas; Inhibit growing of pathogenic bacterium simultaneously, effectively reduce the mosquitos and flies quantity of toilet and plant.
Detailed description of the invention
Preparation method embodiment, concrete steps are as follows:
The first step: Rhodopseudomonas palustris, Candida utilis, bacillus acidophilus, streptomyces griseus, bacillus subtilis are carried out activation culture respectively.
Second step: be inoculated in the culture medium after sterilizing 1 according to the ratio of mass ratio 1:50 by forming mixed bacteria liquid I by Rhodopseudomonas palustris 30wt%, Candida utilis 30wt%, bacillus acidophilus 30wt% and sterilized water 10wt%, cultivates under certain condition and within 3 days, obtains composite bacteria agent capable basis stock solution A in fermentation tank.
Described culture medium 1 is the molasses culture medium improved, and soaks juice 0.5wt% by molasses 1wt%, bean sprout, the sterilized water composition of yeast extract 0.5wt%, sodium citrate 0.2wt%, potassium dihydrogen phosphate 0.1wt% and surplus, and pH value is 6.2; Described fermentation tank is the glass capsulation fermentation tank of transparent controllable temperature; Described certain condition is that fermentation tank is in intensity of illumination 3000lx, temperature is in the environment of 35 DEG C.Described composite bacteria agent capable basis stock solution A is after count plate detects, and wherein Rhodopseudomonas palustris is no less than 1 × 10
8individual/ml, Candida utilis is no less than 1 × 10
8individual/ml, bacillus acidophilus is no less than 2 × 10
8individual/ml.
3rd step: be inoculated in the culture medium after sterilizing 2 according to the ratio of mass ratio 1:50 by forming mixed bacteria liquid II by streptomyces griseus 15wt%, bacillus subtilis 30wt% and sterilized water 55wt%, cultivates under certain condition and within 2 days, obtains composite bacteria agent capable basis stock solution B in fermentation tank.
Described culture medium 2 is made up of soluble starch 1wt%, peptone 0.5wt%, Carnis Bovis seu Bubali cream 0.5wt%, sodium chloride 0.5wt%, trace element solution 1wt%; Described trace element is made up of the sterilized water of magnesium sulfate 0.5wt%, ferrous sulfate 0.1wt%, dipotassium hydrogen phosphate 5% and surplus, and pH value is 7.4; Described fermentation tank is for exposing to the sun filtrated air formula temperature controlled fermentation tank; Described certain condition is that the filtrated air aeration rate of fermentation tank is 15L/min, temperature is 35 DEG C.Described composite bacteria agent capable basis stock solution B is after count plate detects, and wherein streptomyces griseus is no less than 1 × 10
7individual/ml, bacillus subtilis is no less than 2 × 10
8individual/ml.
4th step: by obtain in above-mentioned steps composite bacteria agent capable basis stock solution A 45wt%, composite bacteria agent capable basis stock solution B 45wt%, Fructus Citri Limoniae essence 0.05wt%, citric acid 0.5wt%, glacial acetic acid 0.5 wt% and surplus sterilized water mixing, finally obtain compound microbial deodorizer of the present invention.
Effect example
In hoggery pigsties, implement on-the-spot Deodorization Experiment, pig farm area 500 square metres, site environment temperature is 27 DEG C.Scene is on average divided into three sample area, each sample area arranges a sampled point.Test item is ammonia and hydrogen sulfide gas.According to regulation in " emission standard for odor pollutants " (GB14554-93), ammonia adopts sodium hypochlorite-salicylic acid spectrophotometry, and hydrogen sulfide gas adopts methylene-blue colorimetric method.Before spraying the compound microbial deodorizer in preparation embodiment, respectively the background value carrying out ammonia and hydrogen sulfide gas of three sampled points is measured.
After background value measures, the compound microbial deodorizer in preparation embodiment is diluted ten times, with 150ml/m
2consumption pig house is evenly sprayed.After sprinkling, after 0.5 hour, 2 hours, gas sampling detection is carried out to three sampled points respectively.
Testing result form
According to display effect in form, after using the composite type deodorization agent 0.5h in preparation embodiment, the rate of descent of ammonia is more than 53%, and the rate of descent of hydrogen sulfide is more than 80%.After 2h, all more than 90%.
Hoggery pigsties, before sprinkling, has denseer stink in pig house.After sprinkling, feel without obvious stink.
Claims (2)
1. compound microbial deodorizer and preparation method thereof, is characterized in that: wherein include Rhodopseudomonas palustris (Rhodop seudanonas palustris), Candida utilis (Candida utilis), bacillus acidophilus (Lactobacillus acidophilus), streptomyces griseus (Streptomyces griseus), bacillus subtilis (Bacillus subtilis).
2. the preparation method of a kind of compound microbial deodorizer according to claim 1, it is characterized in that, concrete steps are:
The first step: Rhodopseudomonas palustris, Candida utilis, bacillus acidophilus, streptomyces griseus, bacillus subtilis are carried out activation culture respectively;
Second step: will be that sterilized water forms mixed bacteria liquid I and is inoculated in the culture medium after sterilizing 1 according to the ratio being not less than mass ratio 1:50 by Rhodopseudomonas palustris 20 ~ 30wt%, Candida utilis 20 ~ 30wt%, bacillus acidophilus 20 ~ 30wt% and surplus, cultivates under certain condition and within 2 ~ 3 days, obtains composite bacteria agent capable basis stock solution A in fermentation tank;
Described culture medium 1 is the molasses culture medium improved, and soaks juice 0.5wt% by molasses 1wt%, bean sprout, the sterilized water composition of yeast extract 0.5wt%, sodium citrate 0.2wt%, potassium dihydrogen phosphate 0.1wt% and surplus, and pH value is 6 ~ 7; Described fermentation tank is glass or the acrylic sealing and fermenting tank of transparent controllable temperature; Described certain condition is that fermentation tank is in intensity of illumination 2000 ~ 3000lx, temperature is in the environment of 30 ~ 35 DEG C;
In described composite bacteria agent capable basis stock solution A, Rhodopseudomonas palustris should not be less than 1 × 10
8individual/ml, Candida utilis should not be less than 1 × 10
8individual/ml, bacillus acidophilus should not be less than 2 × 10
8individual/ml;
3rd step: the sterilized water by streptomyces griseus 10 ~ 15wt%, bacillus subtilis 20 ~ 30wt% and surplus is formed mixed bacteria liquid II and is inoculated in the culture medium after sterilizing 2 according to the ratio being not less than mass ratio 1:50, cultivates under certain condition and within 1 ~ 2 day, obtains composite bacteria agent capable basis stock solution B in fermentation tank;
Described culture medium 2 is made up of soluble starch 1wt%, peptone 0.5wt%, Carnis Bovis seu Bubali cream 0.5wt%, sodium chloride 0.5wt%, trace element solution 1wt%; Described trace element is made up of the sterilized water of magnesium sulfate 0.5wt%, ferrous sulfate 0.1wt%, dipotassium hydrogen phosphate 5% and surplus, and pH value is 6 ~ 8; Described fermentation tank is for exposing to the sun filtrated air formula temperature controlled fermentation tank; Described certain condition is that the filtrated air aeration rate of fermentation tank is 10 ~ 20L/min, temperature is 30 ~ 35 DEG C;
In described composite bacteria agent capable basis stock solution B, streptomyces griseus should not be less than 1 × 10
7individual/ml, bacillus subtilis should not be less than 2 × 10
8individual/ml;
4th step: by obtain in above-mentioned steps composite bacteria agent capable basis stock solution A 40 ~ 45wt%, composite bacteria agent capable basis stock solution B 40 ~ 45wt%, essence 0.05wt%, citric acid 0.5wt%, glacial acetic acid 0.5 wt% and surplus sterilized water mixing, finally obtain compound microbial deodorizer of the present invention;
Described essence is conventional essence in this area.
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| CN201410658144.6A CN104840987A (en) | 2014-11-19 | 2014-11-19 | Composite microbial deodorant and preparation method thereof |
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| CN201410658144.6A CN104840987A (en) | 2014-11-19 | 2014-11-19 | Composite microbial deodorant and preparation method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105174491A (en) * | 2015-09-25 | 2015-12-23 | 南京润中生物技术有限公司 | Microbial preparation for water deodorization and preparation method and application thereof |
| CN106282038A (en) * | 2016-08-05 | 2017-01-04 | 湖北凌卓生物工程有限公司 | A kind of composite efficient microbial deodorant and preparation method thereof |
| CN106520597A (en) * | 2016-10-18 | 2017-03-22 | 河南省奶牛生产性能测定中心 | Ecological activity deodorant and preparation method thereof |
| CN106731771A (en) * | 2016-12-19 | 2017-05-31 | 北京清水生态环境工程股份有限公司 | A kind of composite biological deodorant |
| CN107568071A (en) * | 2017-08-30 | 2018-01-12 | 黄山中科新佳生物科技有限公司 | Biological deodorant for livestock and poultry farm |
| CN107653204A (en) * | 2017-10-20 | 2018-02-02 | 上海莱泰生物科技有限公司 | A kind of complex micro organism fungicide for landfill waste processing and preparation method thereof |
| CN108531412A (en) * | 2018-03-20 | 2018-09-14 | 大连三净集团有限公司 | A kind of preparation method of ECMD Efficient Rings control microbial deodorant |
| CN109370934A (en) * | 2018-10-15 | 2019-02-22 | 上海国龙生物科技有限公司 | A kind of aquaculture wastewater processing microbial bacterial agent |
| CN110813075A (en) * | 2019-08-27 | 2020-02-21 | 上海普乐拜格健康科技有限公司 | Biological agent for eliminating peculiar smell of wet garbage and preparation method thereof |
| CN115074265A (en) * | 2022-05-06 | 2022-09-20 | 湖南瑜金环保科技有限公司 | Deodorizing composite microorganism and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000003752A1 (en) * | 1998-07-13 | 2000-01-27 | Life Science Tgo, S.R.L. | Bacteria as odor control agent for carpets |
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102580132A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
-
2014
- 2014-11-19 CN CN201410658144.6A patent/CN104840987A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000003752A1 (en) * | 1998-07-13 | 2000-01-27 | Life Science Tgo, S.R.L. | Bacteria as odor control agent for carpets |
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102580132A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 徐晓军: "《恶臭气体生物净化理论与技术》", 1 May 2005, 化学工业出版社 * |
| 王宜磊: "《微生物学》", 1 February 2010, 化学工业出版社 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105174491A (en) * | 2015-09-25 | 2015-12-23 | 南京润中生物技术有限公司 | Microbial preparation for water deodorization and preparation method and application thereof |
| CN105174491B (en) * | 2015-09-25 | 2017-06-30 | 南京润中生物技术有限公司 | For deodorant microorganism formulation of water body and its preparation method and application |
| CN106282038A (en) * | 2016-08-05 | 2017-01-04 | 湖北凌卓生物工程有限公司 | A kind of composite efficient microbial deodorant and preparation method thereof |
| CN106520597B (en) * | 2016-10-18 | 2020-02-07 | 河南省奶牛生产性能测定中心 | Ecological active deodorant and preparation method thereof |
| CN106520597A (en) * | 2016-10-18 | 2017-03-22 | 河南省奶牛生产性能测定中心 | Ecological activity deodorant and preparation method thereof |
| CN106731771A (en) * | 2016-12-19 | 2017-05-31 | 北京清水生态环境工程股份有限公司 | A kind of composite biological deodorant |
| CN107568071A (en) * | 2017-08-30 | 2018-01-12 | 黄山中科新佳生物科技有限公司 | Biological deodorant for livestock and poultry farm |
| CN107653204A (en) * | 2017-10-20 | 2018-02-02 | 上海莱泰生物科技有限公司 | A kind of complex micro organism fungicide for landfill waste processing and preparation method thereof |
| CN108531412A (en) * | 2018-03-20 | 2018-09-14 | 大连三净集团有限公司 | A kind of preparation method of ECMD Efficient Rings control microbial deodorant |
| CN109370934A (en) * | 2018-10-15 | 2019-02-22 | 上海国龙生物科技有限公司 | A kind of aquaculture wastewater processing microbial bacterial agent |
| CN109370934B (en) * | 2018-10-15 | 2021-11-26 | 上海国龙生物科技有限公司 | Microbial agent for treating aquaculture sewage |
| CN110813075A (en) * | 2019-08-27 | 2020-02-21 | 上海普乐拜格健康科技有限公司 | Biological agent for eliminating peculiar smell of wet garbage and preparation method thereof |
| CN115074265A (en) * | 2022-05-06 | 2022-09-20 | 湖南瑜金环保科技有限公司 | Deodorizing composite microorganism and application thereof |
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