CN109609394A - A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof - Google Patents
A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof Download PDFInfo
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- CN109609394A CN109609394A CN201811596751.9A CN201811596751A CN109609394A CN 109609394 A CN109609394 A CN 109609394A CN 201811596751 A CN201811596751 A CN 201811596751A CN 109609394 A CN109609394 A CN 109609394A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F11/00—Treatment of sludge; Devices therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
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Abstract
A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof, belongs to the technical field of biological materials that microecologic regulator is applied in the environment.The microbial inoculum uses saccharomycete 30 ~ 60%, lactobacillus acidophilus 5 ~ 30%, lactic acid bacteria 5 ~ 30%, bacillus licheniformis 5 ~ 30%, bacillus subtilis 5 ~ 25%.The above strain passes through first class inoculum culture respectively, and two, three grade fermemtation make bacterium number in fermentation liquid reach 2 × 108A/mL.The microbial inoculum is suitable for handling the excreta of farm's animal house and heap fermentation, and the burst size of foul gas can be effectively reduced, provide technical support with environmental protection for being effectively treated for livestock and poultry farm stench.
Description
Technical field
The present invention relates to a kind of deodorization fermentative microorganism microbial inoculums and the preparation method and application thereof, belong to microecologic regulator and exist
The technical field of biological materials applied in environment.
Background technique
With the large-scale development of animal husbandry, farm animal excrement is also increasingly tight to the pollution of farm's ambient enviroment
Weight, wherein generating foul gas is one of major way.The main reason for causing feces of livestock and poultry foul gas to generate is excrement heap
During product the volatilization of the substances such as ammonia, hydrogen sulfide, methane, mercaptan, thioether, indoles, halogenated hydrocarbons, aromatic hydrocarbon and organic acid with release
It puts.Foul gas not only brings the discomfort in smell, but also breathing, digestion, painstaking effort to people to farm environment resident
Pipe, endocrine and nervous system all have an impact to move in odor pollution environment for a long time and can also cause under anorexia, insomnia, memory
The functional diseases such as drop;Sucking stench formaldehyde and benzene have strong carcinogenesis;High concentration stench can also make contactee that edema with the lung involved occur
Swollen even death by suffocation.Therefore, the reasonable processing of farm's foul gas is control farm's environmental sanitation, improves epidemic prevention item
Part and the important link to reduce environmental pollution, and ensure the important measures that animal husbandry develops in a healthy way.
In the prior art, deodorization is mainly carried out using chemical deodorizing agent, generally existing following insufficient: one, chemical deodorizing produces
Product are that essence is added in chemical reagent, are only covered to stink, palliative, do not eliminate stink substances from source
Generation.Two, chemical raw material used by chemical deodorizing agent has certain toxicity to human body and environment.
Summary of the invention
Microbial deodorant provided by the invention includes altogether 5 kinds of microbial bacterias, is respectively as follows: saccharomycete, lactic acid bacteria and lichens
Bacillus, lactobacillus acidophilus, bacillus subtilis.There is good synergistic effect between contained each microbial bacteria.?
Under the synergistic effect, the effect of deodorization can be effectively realized.Specifically, the saccharomycete in microbial bacterial agent being capable of secretion activity object
Matter promotes beneficial microbe growth, itself can generate fragrance;Lactic acid bacteria is able to carry out organic matter fermentation, adjusts pH, inhibits to have
Evil bacterium growth;Bacillus licheniformis, lactobacillus acidophilus, bacillus subtilis can generate enzyme, inhibit the release of foul gas;
When microbial deodorant is sprayed on house refuse and excrement surface, since acidity is presented in microbial deodorant, life can be reduced
The concentration of ammonia, reaches deodorizing effect near rubbish and excrement.
Microbial deodorant of the invention, deodorizing capability is strong, high-efficient, is suitble to house refuse, farm's animal wastes, dirt
The deodorization of each substance such as mud.
Technical solution of the invention is as follows: a kind of deodorization fermentative microorganism microbial inoculum, it is characterised in that: uses saccharomycete
30 ~ 60%, lactobacillus acidophilus 5 ~ 30%, lactic acid bacteria 5 ~ 30%, bacillus licheniformis 5 ~ 30%, bacillus subtilis 5 ~ 25%.
The strain purchase of saccharomycete, lactobacillus acidophilus, lactic acid bacteria, bacillus licheniformis, bacillus subtilis is in unit name
Claim: the industrial Bioisystech Co., Ltd in Hebei Cangzhou, unit address: Hebei province, Hebei Cangzhou, Cang County industry park Xing Jizhen, connection
Phone: 15200830432.
One, a kind of preparation step of deodorization fermentative microorganism microbial inoculum is as follows:
1. inoculation: each strain is connected on slant medium.Saccharomycete cultivation temperature be 30 DEG C, incubation time be 6~
48h;25~30 DEG C of lactic acid bacteria cultivation temperature, incubation time is 6~48h;Bacillus licheniformis cultivation temperature is 25~30 DEG C, training
Supporting the time is 6~48h;25~30 DEG C of bacillus subtilis cultivation temperature, incubation time is 6~48h;Lactobacillus acidophilus culture temperature
Degree is 27~37 DEG C, and incubation time is 6~48h.
2. first class inoculum culture: the strain is inoculated in respectively in the 50mL conical flask for filling fluid nutrient medium, is cultivated
Base pH is 6.0~8.0, and conical flask is put into shaking table oscillation, and temperature is 30 DEG C, vibrates 24~48h;It is counted using blood counting chamber,
Strain quantity respectively reaches 10 in bacterium solution8A/mL;
3. second order fermentation: the strain is inoculated in respectively in the 1000mL conical flask for filling fluid nutrient medium, medium pH 3 ~
6, it is put into shaking table oscillation, temperature is 30 DEG C, vibrates 48~72h;Strain quantity is set to respectively reach 108A/mL or more, by each strain
It is mixed by the mass percent of the strain;
4. three grade fermemtation: mixed bacterium solution being inoculated in the fermentor for filling composite bacteria agent culture medium, composite bacteria agent culture
Base pH3 ~ 6, inoculum concentration are to cultivate 4~7 days at 1~5%, 30 DEG C, and bacterium number in fermentation liquid is made to reach 8~10 × 108A/mL to get
To the complex microbial inoculum.
Three, the preparation method of a kind of complex microorganism additive according to claim 2, it is characterised in that: described
Liquid Culture based formulas are as follows:
1. the Liquid Culture based formulas that saccharomycete uses are as follows: casein peptone 15g/L, yeast 25g/L, 30 g/L of glucose;
2. the Liquid Culture based formulas that lactic acid bacteria uses are as follows: the peptone of 0.5% mass fraction, the corn of 2.0% mass fraction
Slurry, the sucrose of 4.0% mass fraction, the sodium acetate of 0.5% mass fraction, the magnesium sulfate of 0.5% mass fraction, 1.0% mass
The sodium chloride of score, remaining is water;
3. the Liquid Culture based formulas that bacillus licheniformis uses are as follows: the Liquid Culture based formulas that bacillus licheniformis uses are as follows:
Corn pulp 0.45g, peptone 1.50g, it is 1:15, glucose 1.50g that beancake powder, which soaks juice,., initial ph value 7.5.
4. lactobacillus acidophilus use Liquid Culture based formulas are as follows: milk casein peptone 10g, peptone 10g,
Yeast extract 5g, glucose 5g, Tween 80 1g, K2HPO42g, sodium acetate 5g, lemon acid diamine 2g, MgSO4·7H2O
0.2g、MnSO4·H2O 0.05g, distilled water 1000ml;
5. the Liquid Culture based formulas that bacillus subtilis uses are as follows: beef extract 5g, peptone 10g, Nacl 3g, pH 7.0.
Four, deodorization fermentative microorganism microbial inoculum according to claim 1, it is characterised in that: saccharomycete 30 ~ 60% is used,
Lactobacillus acidophilus 5 ~ 30%, lactic acid bacteria 5 ~ 30%, bacillus licheniformis 5 ~ 30%, bacillus subtilis 5 ~ 25%.
The beneficial effects of the present invention present invention is suitable for handling the excreta of farm's animal house and heap fermentation, can effectively drop
The burst size of low foul gas provides technical support with environmental protection for being effectively treated for livestock and poultry farm stench.
Specific embodiment
Technical solution of the invention is as follows: a kind of deodorization fermentative microorganism microbial inoculum, it is characterised in that: uses saccharomycete
30 ~ 60%, lactobacillus acidophilus 5 ~ 30%, lactic acid bacteria 5 ~ 30%, bacillus licheniformis 5 ~ 30%, bacillus subtilis 5 ~ 25%.
Common prescription table
The deodorization fermentative microorganism microbial inoculum obtained according to above-mentioned formula table and preparation method, bacterium number content reach 9 × 108A/mL.
Embodiment 1:
This inspection example is used to investigate the deodorizing capability of deodorization fermentative microorganism microbial inoculum.
By deodorization fermentative microorganism microbial inoculum of the invention, 10 times are diluted with deionized water, mixes 10 minutes, deodorization bacterium is made
Liquid: taking chicken manure from farm at random, respectively takes 10 kilograms, is put into two chambers, every 12 hours sprinkling microbial bacterial agents,
Ammonia and concentration of hydrogen sulfide are detected after 48h.
The experimental results showed that microbial bacterial agent is after spraying, because microbial deodorant is acidity, can capture in air rapidly
Ammonia, reduce stink.
Through detecting, ammonia concentration decline 75% after 48h, concentration of hydrogen sulfide decline 50%, hence it is evident that inhibit the generation of stink.
Embodiment 2
This inspection example is used to investigate the deodorizing capability of deodorization fermentative microorganism microbial inoculum.
Implement live deodorizing test in refuse landfill, meteorological condition is 25 degree of temperature, 3 grades of wind speed or less.In operation area
A sampled point is laid at domain center, sprays the microbial deodorant 100ml that embodiment two is prepared by one square metre, sprinkling is real
Shi Hou, 10 minutes and 40 minutes detection hydrogen sulfide and ammonia rate of descent, to detect deodorizing effect.
It can be seen that the microbial deodorant that the present invention uses, when carrying out deodorization to house refuse, have it is quick and
Deodorizing effect clear advantage.
Embodiment 3
Corrupt house refuse and chicken manure are taken, is mixed according to the ratio of 1:1, then mixture is uniformly mixed with water according to 1:1.5
After filter, collect filtrate to do source of odor use.
Source of odor solution is added into 10ml into each triangular flask, is then respectively connected to each strain 10ml, 30 DEG C,
Detect the deodorizing capability of each bacterium under conditions of 150rpm after culture 48h with odor threshold method.
Experimental method: taking 1ml fermentation liquid in small beaker, then adds deionization into small beaker with the amount of each 10ml
Water, until can't smell stink, the diluted multiple of record institute, the preliminary judgment criteria as the screening of deodorization bacterium.Experimental result is as follows
Shown in table.
In upper table, saccharomycete, lactic acid bacteria and the bacillus tested are the correspondence one that two step 2 of embodiment obtains
Grade seed.And 5 kinds of bacterium are mixed into the microbial deodorant that embodiment two is finally prepared.
As can be seen from the above data, the degradation of the saccharomycete screened, lactic acid bacteria and bacillus to stink substances
Rate all 80% hereinafter, but 5 kinds of bacterium the order of magnitude equal percentage mixing after 95.2% is up to the removal rate of stink substances, thus
After sufficiently demonstrating 5 kinds of bacterium mixing, synergistic effect is mutually played, to improve deodorizing effect.
Claims (4)
1. a kind of deodorization fermentative microorganism microbial inoculum, it is characterised in that: in total strain number ratio in the microbial inoculum are as follows: saccharomycete 30 ~
60%, lactobacillus acidophilus 5 ~ 30%, lactic acid bacteria 5 ~ 30%, bacillus licheniformis 5 ~ 30%, bacillus subtilis 5 ~ 25%.
2. a kind of preparation method of complex microorganism additive according to claim 1, it is characterised in that: described compound micro-
The preparation step of bio-additive is as follows:
Inoculation: each strain is connected on slant medium;Saccharomycete cultivation temperature is 30 DEG C, and incubation time is 6~48h;
30~36 DEG C of lactic acid bacteria cultivation temperature, incubation time is 6~48h;Bacillus licheniformis cultivation temperature is 30~36 DEG C, when culture
Between be 6~48h;30~36 DEG C of bacillus subtilis cultivation temperature, incubation time is 6~48h;Lactobacillus acidophilus cultivation temperature is
27~37 DEG C, incubation time is 6~48h;
First class inoculum culture: the strain is inoculated in respectively in the 50mL conical flask for filling fluid nutrient medium, and medium pH is
6.0~8.0, conical flask is put into shaking table oscillation, and temperature is 36 DEG C, vibrates 24~48h;It is counted using blood counting chamber, in bacterium solution
Strain quantity respectively reaches 108A/mL;
Second order fermentation: the strain is inoculated in respectively in the 1000mL conical flask for filling fluid nutrient medium, medium pH 3 ~ 6,
It is put into shaking table oscillation, temperature is 30 DEG C, vibrates 48~72h;Strain quantity is set to respectively reach 108A/mL or more presses each strain
The mass percent of the strain mixes;
Three grade fermemtation: mixed bacterium solution is inoculated in the fermentor for filling composite bacteria agent culture medium, composite bacteria agent culture medium
PH3 ~ 6, inoculum concentration are to cultivate 4~7 days at 1~5%, 30 DEG C, and bacterium number in fermentation liquid is made to reach 8~10 × 108A/mL is to get arriving
The complex microbial inoculum.
3. a kind of preparation method of complex microorganism additive according to claim 2, it is characterised in that: the liquid training
Support based formulas are as follows:
The Liquid Culture based formulas that the saccharomycete uses are as follows: casein peptone 15g/L, yeast 25g/L, 30 g/L of glucose;
The Liquid Culture based formulas that the lactic acid bacteria uses are as follows: the peptone of 0.5% mass fraction, the jade of 2.0% mass fraction
Rice & peanut milk, the sucrose of 4.0% mass fraction, the sodium acetate of 0.5% mass fraction, the magnesium sulfate of 0.5% mass fraction, 1.0% matter
The sodium chloride of score is measured, remaining is water;
The Liquid Culture based formulas that the bacillus licheniformis uses are as follows: the Liquid Culture based formulas that bacillus licheniformis uses
Are as follows: corn pulp 0.45g, peptone 1.50g, it is 1:15, glucose 1.50g that beancake powder, which soaks juice,;, initial ph value 7.5;
The Liquid Culture based formulas that the lactobacillus acidophilus uses are as follows: milk casein peptone 10g, peptone 10g,
Yeast extract 5g, glucose 5g, Tween 80 1g, K2HPO4 2g, sodium acetate 5g, lemon acid diamine 2g, MgSO4·7H2O
0.2g、MnSO4·H2O 0.05g, distilled water 1000ml;
The Liquid Culture based formulas that the bacillus subtilis uses are as follows: beef extract 5g, peptone 10g, Nacl 3g, pH
7.0。
4. a kind of deodorization fermentative microorganism microbial inoculum according to claim 1, it is characterised in that: the bacteria agent is used for environment
Deodorization.
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Cited By (5)
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CN110172418A (en) * | 2019-05-10 | 2019-08-27 | 浙江九田环保科技有限公司 | A kind of dedicated Bacteria Culture particular solution formula of deodorization and preparation method thereof |
CN110343649A (en) * | 2019-08-23 | 2019-10-18 | 浙江中益生物环保科技有限公司 | A kind of microbial deoderizer and its application |
CN113599998A (en) * | 2021-07-20 | 2021-11-05 | 上海野马环保设备工程有限公司 | Preparation method of wet garbage compost biological deodorant |
CN114632411A (en) * | 2022-03-30 | 2022-06-17 | 江苏省农业科学院 | Carbon nitrogen emission reduction method applied to urine, excrement and sewage in pig farm |
CN114632411B (en) * | 2022-03-30 | 2024-10-22 | 江苏省农业科学院 | Method for reducing carbon and nitrogen emissions of urine, foam manure and manure applied to pig farm |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110172418A (en) * | 2019-05-10 | 2019-08-27 | 浙江九田环保科技有限公司 | A kind of dedicated Bacteria Culture particular solution formula of deodorization and preparation method thereof |
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CN113599998A (en) * | 2021-07-20 | 2021-11-05 | 上海野马环保设备工程有限公司 | Preparation method of wet garbage compost biological deodorant |
CN114632411A (en) * | 2022-03-30 | 2022-06-17 | 江苏省农业科学院 | Carbon nitrogen emission reduction method applied to urine, excrement and sewage in pig farm |
CN114632411B (en) * | 2022-03-30 | 2024-10-22 | 江苏省农业科学院 | Method for reducing carbon and nitrogen emissions of urine, foam manure and manure applied to pig farm |
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